1,116 results on '"Ferrazzano A"'
Search Results
2. The value of ACR, European, Korean, and ATA ultrasound risk stratification systems combined with RAS mutations for detecting thyroid carcinoma in cytologically indeterminate and suspicious for malignancy thyroid nodules
- Author
-
Scappaticcio, Lorenzo, Di Martino, Nicole, Caruso, Paola, Ferrazzano, Pamela, Marino, Federica Zito, Clery, Eduardo, Cioce, Alessandro, Cozzolino, Giovanni, Maiorino, Maria Ida, Docimo, Giovanni, Trimboli, Pierpaolo, Franco, Renato, Esposito, Katherine, and Bellastella, Giuseppe
- Published
- 2024
- Full Text
- View/download PDF
3. Prediction of classical versus non classical papillary thyroid carcinoma subtypes from cytology of nodules classified according to TIRADS
- Author
-
Scappaticcio, Lorenzo, Trimboli, Pierpaolo, Bellastella, Giuseppe, Ferrazzano, Pamela, Clery, Eduardo, Cozzolino, Immacolata, Montella, Marco, Fasano, Morena, Pirozzi, Mario, Ferrandes, Sonia, Docimo, Giovanni, Ciardiello, Fortunato, Franco, Renato, and Esposito, Katherine
- Published
- 2024
- Full Text
- View/download PDF
4. Does thyroid diseases contribute to the natural history of idiopathic adult-onset dystonia? Data from the Italian Dystonia Registry
- Author
-
Idrissi, Sarah, Velucci, Vittorio, Esposito, Marcello, Trinchillo, Assunta, Habestwallner, Francesco, Belvisi, Daniele, Fabbrini, Giovanni, Ferrazzano, Gina, Rizzo, Vincenzo, Terranova, Carmen, Girlanda, Paolo, Pellicciari, Roberta, Avanzino, Laura, Di Biasio, Francesca, Marchese, Roberta, Bono, Francesco, Idone, Giovanni, Laterza, Vincenzo, Lettieri, Christian, Rinaldo, Sara, Eleopra, Roberto, Castagna, Anna, Altavista, Maria Concetta, Moschella, Vincenzo, Erro, Roberto, Barone, Paolo, Barbero, Pierangelo, Ceravolo, Roberto, Mazzucchi, Sonia, Mascia, Marcello Mario, Ercoli, Tommaso, Muroni, Antonella, Zibetti, Maurizio, Lopiano, Leonardo, Scaglione, Cesa Lorella Maria, Bentivoglio, Anna Rita, Petracca, Martina, Magistrelli, Luca, Cotelli, Maria Sofia, Cossu, Giovanni, Squintani, Giovanna Maddalena, De Santis, Tiziana, Schirinzi, Tommaso, Misceo, Salvatore, Pisani, Antonio, Berardelli, Alfredo, and Defazio, Giovanni
- Published
- 2024
- Full Text
- View/download PDF
5. Insight into motor fatigue mechanisms in natalizumab treated multiple sclerosis patients with wearing off
- Author
-
Giorgio Leodori, Marco Mancuso, Davide Maccarrone, Matteo Tartaglia, Antonio Ianniello, Francesco Certo, Gina Ferrazzano, Leonardo Malimpensa, Daniele Belvisi, Carlo Pozzilli, Alfredo Berardelli, and Antonella Conte
- Subjects
Motor fatigue ,Natalizumab ,Wearing-off ,Neurophysiology ,TMS-EEG ,Sensorimotor network ,Medicine ,Science - Abstract
Abstract Motor fatigue in Multiple Sclerosis (MS) is due to reduced motor cortex (M1) output and altered sensorimotor network (SMN) modulation. Natalizumab, a disease-modifying therapy, reduces neuroinflammation and improves fatigue. However, some patients treated with natalizumab experience fatigue recurrence (‘wearing-off’) before subsequent infusions. Wearing-off provides a valuable window into MS-related motor fatigue mechanisms in a controlled, clinically stable, setting. This study investigates whether wearing-off is associated with worsening motor fatigue and its neurophysiological mechanisms and assesses natalizumab’s effect on MS-related fatigue. Forty-five relapsing–remitting MS patients with wearing-off symptoms were evaluated pre- and post-natalizumab infusion. Assessments included evaluating disability levels, depressive symptoms, and the impact of fatigue symptoms on cognitive, physical, and psychosocial functioning. The motor fatigue index was computed through the number of blocks completed during a fatiguing task and peripheral, central, and supraspinal fatigue (M1 output) were evaluated by measuring the superimposed twitches evoked by peripheral nerve and transcranial magnetic stimulation of M1. Transcranial magnetic stimulation-electroencephalography assessed M1 effective connectivity by measuring TMS-evoked potentials (TEPs) within the SMN before- and after the task. We found that wearing-off was associated with increased motor fatigue index, increased central and supraspinal fatigue, and diminished task-related modulation of TEPs compared to post-natalizumab infusion. Wearing-off was also associated with worsened fatigue impact and depression symptom scores. We conclude that the wearing-off phenomenon is associated with worsening motor fatigue due to altered M1 output and modulation of the SMN. Motor fatigue in MS may reflect reversible, inflammation-related changes in the SMN that natalizumab can modulate. Our findings apply primarily to MS patients receiving natalizumab, emphasizing the need for further research on other treatments with wearing-off.
- Published
- 2024
- Full Text
- View/download PDF
6. Insight into motor fatigue mechanisms in natalizumab treated multiple sclerosis patients with wearing off
- Author
-
Leodori, Giorgio, Mancuso, Marco, Maccarrone, Davide, Tartaglia, Matteo, Ianniello, Antonio, Certo, Francesco, Ferrazzano, Gina, Malimpensa, Leonardo, Belvisi, Daniele, Pozzilli, Carlo, Berardelli, Alfredo, and Conte, Antonella
- Published
- 2024
- Full Text
- View/download PDF
7. TrkB-mediated neuroprotection in female hippocampal neurons is autonomous, estrogen receptor alpha-dependent, and eliminated by testosterone: a proposed model for sex differences in neonatal hippocampal neuronal injury
- Author
-
Chanana, Vishal, Zafer, Dila, Kintner, Douglas B, Chandrashekhar, Jayadevi H, Eickhoff, Jens, Ferrazzano, Peter A, Levine, Jon E, and Cengiz, Pelin
- Published
- 2024
- Full Text
- View/download PDF
8. TrkB-mediated sustained neuroprotection is sex-specific and ERα-dependent in adult mice following neonatal hypoxia ischemia
- Author
-
Chanana, Vishal, Hackett, Margaret, Deveci, Nazli, Aycan, Nur, Ozaydin, Burak, Cagatay, Nur Sena, Hanalioglu, Damla, Kintner, Douglas B., Corcoran, Karson, Yapici, Sefer, Camci, Furkan, Eickhoff, Jens, Frick, Karyn M., Ferrazzano, Peter, Levine, Jon E., and Cengiz, Pelin
- Published
- 2024
- Full Text
- View/download PDF
9. Thymic hyperplasia is accurate to detect new-onset Graves’ hyperthyroidism and resolves after restoring euthyroidism
- Author
-
Scappaticcio, L., Caruso, P., Di Martino, N., Ferrazzano, P., Clemente, A., Maiorino, M. I., Regginelli, A., Docimo, G., Rambaldi, P. F., Bellastella, G., Trimboli, P., Cappabianca, S., and Esposito, K.
- Published
- 2024
- Full Text
- View/download PDF
10. Multi-modal MRI of hippocampal morphometry and connectivity after pediatric severe TBI
- Author
-
Guerrero-Gonzalez, Jose M., Kirk, Gregory R., Birn, Rasmus, Bigler, Erin D., Bowen, Katherine, Broman, Aimee T., Rosario, Bedda L., Butt, Warwick, Beers, Sue R., Bell, Michael J., Alexander, Andrew L., and Ferrazzano, Peter A.
- Published
- 2024
- Full Text
- View/download PDF
11. TrkB-mediated neuroprotection in female hippocampal neurons is autonomous, estrogen receptor alpha-dependent, and eliminated by testosterone: a proposed model for sex differences in neonatal hippocampal neuronal injury
- Author
-
Vishal Chanana, Dila Zafer, Douglas B Kintner, Jayadevi H Chandrashekhar, Jens Eickhoff, Peter A Ferrazzano, Jon E Levine, and Pelin Cengiz
- Subjects
Neurotrophin receptor ,Estrogen receptor alpha ,Neonatal ,Hypoxia ischemia ,7,8-dihydroxyflavone ,Tyrosine kinase B receptor ,Medicine ,Physiology ,QP1-981 - Abstract
Highlights Following in vitro ischemia, the nerve growth factor receptor TrkB is activated in the presence of the TrkB agonist 7,8-DHF only in female and not in male cultured hippocampal neurons, leading to increased neuronal survival. Expression of ERα is increased following in vitro ischemia in female but not male hippocampal neurons. The female hippocampal neuronal specific responses to in vitro ischemia are blocked by pre-treatment with testosterone. The data support a model for a female-specific a neuroprotective pathway in hippocampal neurons. The pathway is activated by a TrkB agonist, dependent on ERα and blocked by testosterone.
- Published
- 2024
- Full Text
- View/download PDF
12. Corrigendum: Post-discharge outcomes of hospitalized children diagnosed with acute SARS-CoV-2 or MIS-C
- Author
-
Ericka L. Fink, Alicia M. Alcamo, Marlina Lovett, Mary Hartman, Cydni Williams, Angela Garcia, Lindsey Rasmussen, Ria Pal, Kurt Drury, Elizabeth MackDiaz, Peter A. Ferrazzano, Leslie Dervan, Brian Appavu, Kellie Snooks, Casey Stulce, Pamela Rubin, Bianca Pate, Nicole Toney, Courtney L. Robertson, Mark S. Wainwright, Juan D. Roa, Michelle E. Schober, and Beth S. Slomine
- Subjects
pediatrics ,SARS-CoV-2 ,child development ,patient outcome assessment ,post-acute COVID-19 syndrome ,Pediatrics ,RJ1-570 - Published
- 2024
- Full Text
- View/download PDF
13. TrkB-mediated sustained neuroprotection is sex-specific and $$\text{ER}\alpha$$ ER α -dependent in adult mice following neonatal hypoxia ischemia
- Author
-
Vishal Chanana, Margaret Hackett, Nazli Deveci, Nur Aycan, Burak Ozaydin, Nur Sena Cagatay, Damla Hanalioglu, Douglas B. Kintner, Karson Corcoran, Sefer Yapici, Furkan Camci, Jens Eickhoff, Karyn M. Frick, Peter Ferrazzano, Jon E. Levine, and Pelin Cengiz
- Subjects
Sex differences ,Hypoxic ischemic encephalopathy ,Hypoxia ,Ischemia ,Neonate ,Brain injury ,Medicine ,Physiology ,QP1-981 - Abstract
Abstract Background Neonatal hypoxia ischemia (HI) related brain injury is one of the major causes of life-long neurological morbidities that result in learning and memory impairments. Evidence suggests that male neonates are more susceptible to the detrimental effects of HI, yet the mechanisms mediating these sex-specific responses to neural injury in neonates remain poorly understood. We previously tested the effects of treatment with a small molecule agonist of the tyrosine kinase B receptor (TrkB), 7,8-dihydroxyflavone (DHF) following neonatal HI and determined that females, but not males exhibit increased phosphorylation of TrkB and reduced apoptosis in their hippocampi. Moreover, these female-specific effects of the TrkB agonist were found to be dependent upon the expression of $$\text{ER}\alpha$$ ER α . These findings demonstrated that TrkB activation in the presence of $$\text{ER}\alpha$$ ER α comprises one pathway by which neuroprotection may be conferred in a female-specific manner. The goal of this study was to determine the role of $$\text{ER}\alpha$$ ER α -dependent TrkB-mediated neuroprotection in memory and anxiety in young adult mice exposed to HI during the neonatal period. Methods In this study, we used a unilateral hypoxic ischemic (HI) mouse model. $$\text{ER}\alpha$$ ER α +/+ or $$\text{ER}\alpha$$ ER α −/− mice were subjected to HI on postnatal day (P) 9 and mice were treated with either vehicle control or the TrkB agonist, DHF, for 7 days following HI. When mice reached young adulthood, we used the novel object recognition, novel object location and open field tests to assess long-term memory and anxiety-like behavior. The brains were then assessed for tissue damage using immunohistochemistry. Results Neonatal DHF treatment prevented HI-induced decrements in recognition and location memory in adulthood in females, but not in males. This protective effect was absent in female mice lacking $$\text{ER}\alpha$$ ER α . The female-specific improved recognition and location memory outcomes in adulthood conferred by DHF therapy after neonatal HI tended to be or were $$\text{ER}\alpha$$ ER α -dependent, respectively. Interestingly, DHF triggered anxiety-like behavior in both sexes only in the mice that lacked $$\text{ER}\alpha$$ ER α . When we assessed the severity of injury, we found that DHF therapy did not decrease the percent tissue loss in proportion to functional recovery. We additionally observed that the presence of $$\text{ER}\alpha$$ ER α significantly reduced overall HI-associated mortality in both sexes. Conclusions These observations provide evidence for a therapeutic role for DHF in which TrkB-mediated sustained recovery of recognition and location memories in females are $$\text{ER}\alpha$$ ER α -associated and dependent, respectively. However, the beneficial effects of DHF therapy did not include reduction of gross tissue loss but may be derived from the enhanced functioning of residual tissues in a cell-specific manner.
- Published
- 2024
- Full Text
- View/download PDF
14. The multidimensional assessment of body representation and interoception in multiple sclerosis
- Author
-
Raimo, Simona, Ferrazzano, Gina, Di Vita, Antonella, Gaita, Mariachiara, Satriano, Federica, Veneziano, Miriam, Torchia, Valentina, Zerella, Maria Paola, Malimpensa, Leonardo, Signoriello, Elisabetta, Lus, Giacomo, Palermo, Liana, and Conte, Antonella
- Published
- 2024
- Full Text
- View/download PDF
15. Validated Questionnaire on Intraoral Scanner Effectiveness in Paediatric Patients
- Author
-
Silvia Caruso, Giulia Di Benedetto, Pierluigi Pelagalli, Luca Caruso, Sara Caruso, Antonella Mattei, Fabiana Fiasca, Roberto Gatto, Gianmaria Fabrizio Ferrazzano, and Elias Berdouses
- Subjects
intraoral scanner ,pediatric dentistry ,questionnaire ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
The intraoral scanner represents an important innovation in the world of dentistry, capable of transforming and digitizing conventional workflows and significantly improving communication with the patient, especially in pediatric age. The aim of this study is to investigate how digital dentistry, using the intraoral scanner, can interact in the communicative trinomial dentist–pediatric patient–parents and how this tool can be useful in communicating and understanding the received message. The value of the introduction of digital dentistry on communication schemes is evaluated by administering a questionnaire of 17 questions. A sample of 100 children aged between 6 and 12 years was studied. Of the sample of children/parents included in the study, 50 were re-examined 4 weeks after the first visit to re-administer the questionnaire. Internal consistency, assessed through Cronbach’s alpha, was adequate, being alpha equal to 0.728. Test–retest reliability was assessed with Cohen’s kappa index for dichotomous variables (percentage of agreement was >85.71%), and for the discrete variables it was assessed with the Intraclass Correlation Coefficient (ICC). The reliability was moderate (0.5 < ICC < 0.75) for Q4, Q9, Q11 and Q1; for the other answers, the reliability was poor (ICC < 0.5) and not statistically significant. The digital approach is confirmed not only as a powerful innovation from an engineering or clinical point of view, but also as a useful tool for a more effective communication in the approach to pediatric patients.
- Published
- 2024
- Full Text
- View/download PDF
16. Post-discharge outcomes of hospitalized children diagnosed with acute SARS-CoV-2 or MIS-C
- Author
-
Ericka L. Fink, Alicia M. Alcamo, Marlina Lovett, Mary Hartman, Cydni Williams, Angela Garcia, Lindsey Rasmussen, Ria Pal, Kurt Drury, Elizabeth MackDiaz, Peter A. Ferrazzano, Leslie Dervan, Brian Appavu, Kellie Snooks, Casey Stulce, Pamela Rubin, Bianca Pate, Nicole Toney, Courtney L. Robertson, Mark S. Wainwright, Juan D. Roa, Michelle E. Schober, and Beth S. Slomine
- Subjects
pediatrics ,SARS-CoV-2 ,child development ,patient outcome assessment ,post-acute COVID-19 syndrome ,Pediatrics ,RJ1-570 - Abstract
IntroductionHospitalized children diagnosed with SARS-CoV-2-related conditions are at risk for new or persistent symptoms and functional impairments. Our objective was to analyze post-hospital symptoms, healthcare utilization, and outcomes of children previously hospitalized and diagnosed with acute SARS-CoV-2 infection or Multisystem Inflammatory Syndrome in Children (MIS-C).MethodsProspective, multicenter electronic survey of parents of children
- Published
- 2024
- Full Text
- View/download PDF
17. Exploring miRNAs’ Based Modeling Approach for Predicting PIRA in Multiple Sclerosis: A Comprehensive Analysis
- Author
-
Tommaso Gosetti di Sturmeck, Leonardo Malimpensa, Gina Ferrazzano, Daniele Belvisi, Giorgio Leodori, Flaminia Lembo, Rossella Brandi, Esterina Pascale, Antonino Cattaneo, Marco Salvetti, Antonella Conte, Mara D’Onofrio, and Ivan Arisi
- Subjects
progression independent of relapse activity (PIRA) ,multiple sclerosis ,modeling approach ,miRNA ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The current hypothesis on the pathophysiology of multiple sclerosis (MS) suggests the involvement of both inflammatory and neurodegenerative mechanisms. Disease Modifying Therapies (DMTs) effectively decrease relapse rates, thus reducing relapse-associated disability in people with MS. In some patients, disability progression, however, is not solely linked to new lesions and clinical relapses but can manifest independently. Progression Independent of Relapse Activity (PIRA) significantly contributes to long-term disability, stressing the urge to unveil biomarkers to forecast disease progression. Twenty-five adult patients with relapsing–remitting multiple sclerosis (RRMS) were enrolled in a cohort study, according to the latest McDonald criteria, and tested before and after high-efficacy Disease Modifying Therapies (DMTs) (6–24 months). Through Agilent microarrays, we analyzed miRNA profiles from peripheral blood mononuclear cells. Multivariate logistic and linear models with interactions were generated. Robustness was assessed by randomization tests in R. A subset of miRNAs, correlated with PIRA, and the Expanded Disability Status Scale (EDSS), was selected. To refine the patient stratification connected to the disease trajectory, we computed a robust logistic classification model derived from baseline miRNA expression to predict PIRA status (AUC = 0.971). We built an optimal multilinear model by selecting four other miRNA predictors to describe EDSS changes compared to baseline. Multivariate modeling offers a promising avenue to uncover potential biomarkers essential for accurate prediction of disability progression in early MS stages. These models can provide valuable insights into developing personalized and effective treatment strategies.
- Published
- 2024
- Full Text
- View/download PDF
18. Efficacy of F-ACP-Containing Dental Mousse in the Remineralization of White Spot Lesions after Fixed Orthodontic Therapy: A Randomized Clinical Trial
- Author
-
Massimiliano Ciribè, Erika Cirillo, Martina Mammone, Giulia Vallogini, Paola Festa, Simone Piga, Gianmaria Fabrizio Ferrazzano, and Angela Galeotti
- Subjects
white spot lesion ,fixed orthodontic therapy ,remineralization ,caries ,prevention ,Biology (General) ,QH301-705.5 - Abstract
Fixed appliance (FA) therapy predisposes patients to white spot lesions (WSLs). The F-ACP complex (amorphous calcium phosphate nanoparticles enriched with carbonate and fluorine and coated with citrate) has been effective for in vitro enamel remineralization. The aim of this study was to evaluate the efficacy of the F-ACP complex in remineralizing WSLs after FA therapy. One hundred and six adolescents (aged 12–20 years) were randomized into study and control groups after FA therapy. Patients in the study group were advised to use dental mousse containing F-ACP applied within Essix retainers for six months. The presence of WSLs was recorded at baseline (T0), 3 months (T1), and 6 months (T2) according to the International Caries Detection and Assessment System (ICDAS). Visual Plaque Index (VPI) and Gingival Bleeding Index (GBI) were recorded. Among 106 study participants, 91 (52 and 39 in study and control groups, respectively) completed the study. The results showed that the ICDAS score was significantly lower (p < 0.001) in the study group than in the control group between T0 and T2. The application of mousse containing the F-ACP complex inside Essix retainers for six months is effective in remineralizing white spot lesions in patients after FA therapy without side effects.
- Published
- 2024
- Full Text
- View/download PDF
19. A Journey into the Evolution of Human Host-Oral Microbiome Relationship through Ancient Dental Calculus: A Scoping Review
- Author
-
Alessandra Putrino, Enrico Marinelli, Angela Galeotti, Gianmaria Fabrizio Ferrazzano, Massimiliano Ciribè, and Simona Zaami
- Subjects
dental calculus ,palaeomicrobiology ,oral microbiome ,tooth decay ,periodontal disease ,oral health ,Biology (General) ,QH301-705.5 - Abstract
One of the most promising areas of research in palaeomicrobiology is the study of the human microbiome. In particular, ancient dental calculus helps to reconstruct a substantial share of oral microbiome composition by mapping together human evolution with its state of health/oral disease. This review aims to trace microbial characteristics in ancient dental calculus to describe the evolution of the human host-oral microbiome relationship in oral health or disease in children and adults. Following the PRISMA-Extension for Scoping Reviews guidelines, the main scientific databases (PubMed, Scopus, Lilacs, Cochrane Library) have been drawn upon. Eligibility criteria were established, and all the data collected on a purpose-oriented collection form were analysed descriptively. From the initial 340 records, only 19 studies were deemed comprehensive enough for the purpose of this review. The knowledge of the composition of ancient oral microbiomes has broadened over the past few years thanks to increasingly well-performing decontamination protocols and additional analytical avenues. Above all, metagenomic sequencing, also implemented by state-of-the-art bioinformatics tools, allows for the determination of the qualitative-quantitative composition of microbial species associated with health status and caries/periodontal disease. Some microbial species, especially periodontal pathogens, do not appear to have changed in history, while others that support caries disease or oral health could be connected to human evolution through lifestyle and environmental contributing factors.
- Published
- 2024
- Full Text
- View/download PDF
20. Cladribine and ocrelizumab induce differential miRNA profiles in peripheral blood mononucleated cells from relapsing–remitting multiple sclerosis patients
- Author
-
Ivan Arisi, Leonardo Malimpensa, Valeria Manzini, Rossella Brandi, Tommaso Gosetti di Sturmeck, Chiara D’Amelio, Sebastiano Crisafulli, Gina Ferrazzano, Daniele Belvisi, Francesca Malerba, Rita Florio, Esterina Pascale, Hermona Soreq, Marco Salvetti, Antonino Cattaneo, Mara D’Onofrio, and Antonella Conte
- Subjects
multiple sclerosis ,cladribine ,ocrelizumab ,DMT ,biomarker ,miRNA ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Background and objectivesMultiple sclerosis (MS) is a chronic, progressive neurological disease characterized by early-stage neuroinflammation, neurodegeneration, and demyelination that involves a spectrum of heterogeneous clinical manifestations in terms of disease course and response to therapy. Even though several disease-modifying therapies (DMTs) are available to prevent MS-related brain damage—acting on the peripheral immune system with an indirect effect on MS lesions—individualizing therapy according to disease characteristics and prognostic factors is still an unmet need. Given that deregulated miRNAs have been proposed as diagnostic tools in neurodegenerative/neuroinflammatory diseases such as MS, we aimed to explore miRNA profiles as potential classifiers of the relapsing–remitting MS (RRMS) patients’ prospects to gain a more effective DMT choice and achieve a preferential drug response.MethodsA total of 25 adult patients with RRMS were enrolled in a cohort study, according to the latest McDonald criteria before (pre-cladribine, pre-CLA; pre-ocrelizumab, pre-OCRE, time T0) and after high-efficacy DMTs, time T1, 6 months post-CLA (n = 10, 7 F and 3 M, age 39.0 ± 7.5) or post-OCRE (n = 15, 10 F and 5 M, age 40.5 ± 10.4) treatment. A total of 15 age- and sex-matched healthy control subjects (9 F and 6 M, age 36.3 ± 3.0) were also selected. By using Agilent microarrays, we analyzed miRNA profiles from peripheral blood mononuclear cells (PBMC). miRNA–target networks were obtained by miRTargetLink, and Pearson’s correlation served to estimate the association between miRNAs and outcome clinical features.ResultsFirst, the miRNA profiles of pre-CLA or pre-OCRE RRMS patients compared to healthy controls identified modulated miRNA patterns (40 and seven miRNAs, respectively). A direct comparison of the two pre-treatment groups at T0 and T1 revealed more pro-inflammatory patterns in the pre-CLA miRNA profiles. Moreover, both DMTs emerged as being capable of reverting some dysregulated miRNAs toward a protective phenotype. Both drug-dependent miRNA profiles and specific miRNAs, such as miR-199a-3p, miR-29b-3p, and miR-151a-3p, emerged as potentially involved in these drug-induced mechanisms. This enabled the selection of miRNAs correlated to clinical features and the related miRNA–mRNA network.DiscussionThese data support the hypothesis of specific deregulated miRNAs as putative biomarkers in RRMS patients’ stratification and DMT drug response.
- Published
- 2023
- Full Text
- View/download PDF
21. Do cerebrovascular risk factors impact the clinical expression of idiopathic isolated adult-onset dystonia?
- Author
-
Zaccone, Claudio, Cerne, Denise, Idone, Giovanni, Ferrazzano, Gina, Rinaldo, Sara, Humaidan, Kais, Devigili, Grazia, Polidori, Luigi, Mazzucchi, Sonia, Ramella, Marina, Ledda, Claudia, Petracca, Martina, Oppo, Valentina, Contaldi, Elena, Turla, Marinella, Gigante, Angelo Fabio, Valentino, Francesca, Cassano, Daniela, Modugno, Nicola, Tambasco, Nicola, Aguggia, Marco, Romano, Marcello, Marinelli, Lucio, Mascia, Marcello Mario, Belvisi, Daniele, Esposito, Marcello, Pellicciari, Roberta, Trinchillo, Assunta, Terranova, Carmen, Bertino, Salvatore, Avanzino, Laura, Di Biasio, Francesca, Bono, Francesco, Laterza, Vincenzo, Lettieri, Christian, Eleopra, Roberto, Fabbrini, Giovanni, Barbero, Pierangelo, Bertolasi, Laura, Altavista, Maria Concetta, Erro, Roberto, Ceravolo, Roberto, Castagna, Anna, Zibetti, Maurizio, Bentivoglio, Anna Rita, Cossu, Giovanni, Magistrelli, Luca, Scaglione, Cesa, Albanese, Alberto, Cotelli, Maria Sofia, Misceo, Salvatore, Pisani, Antonio, Schirinzi, Tommaso, Maderna, Luca, Squintani, Giovanna, Berardelli, Alfredo, and Defazio, Giovanni
- Published
- 2023
- Full Text
- View/download PDF
22. Diagnostic and therapeutic recommendations in adult dystonia: a joint document by the Italian Society of Neurology, the Italian Academy for the Study of Parkinson’s Disease and Movement Disorders, and the Italian Network on Botulinum Toxin
- Author
-
Romano, Marcello, Bagnato, Sergio, Altavista, Maria Concetta, Avanzino, Laura, Belvisi, Daniele, Bologna, Matteo, Bono, Francesco, Carecchio, Miryam, Castagna, Anna, Ceravolo, Roberto, Conte, Antonella, Cosentino, Giuseppe, Eleopra, Roberto, Ercoli, Tommaso, Esposito, Marcello, Fabbrini, Giovanni, Ferrazzano, Gina, Lalli, Stefania, Mascia, Marcello Maria, Osio, Maurizio, Pellicciari, Roberta, Petrucci, Simona, Valente, Enza Maria, Valentino, Francesca, Zappia, Mario, Zibetti, Maurizio, Girlanda, Paolo, Tinazzi, Michele, Defazio, Giovanni, and Berardelli, Alfredo
- Published
- 2022
- Full Text
- View/download PDF
23. Do demographic and clinical features and comorbidities affect the risk of spread to an additional body site in functional motor disorders?
- Author
-
Ercoli, Tommaso, Tinazzi, Michele, Geroin, Christian, Marcuzzo, Enrico, Erro, Roberto, Cuoco, Sofia, Ceravolo, Roberto, Mazzucchi, Sonia, Pilotto, Andrea, Padovani, Alessandro, Romito, Luigi Michele, Eleopra, Roberto, Zappia, Mario, Nicoletti, Alessandra, Dallocchio, Carlo, Arbasino, Carla, Bono, Francesco, Spano, Giorgio, Demartini, Benedetta, Gambini, Orsola, Modugno, Nicola, Olivola, Enrica, Bonanni, Laura, Albanese, Alberto, Ferrazzano, Gina, Tessitore, Alessandro, Lopiano, Leonardo, Calandra-Buonaura, Giovanna, Petracca, Martina, Morgante, Francesca, Esposito, Marcello, Pisani, Antonio, Manganotti, Paolo, Tesolin, Lucia, Teatini, Francesco, Stocchi, Fabrizio, and Defazio, Giovanni
- Published
- 2022
- Full Text
- View/download PDF
24. Clinical Efficacy and Tolerability of a New Experimental Mucoadhesive Patch for Topical Anesthesia of Oral Mucosa in Pediatric Dentistry
- Author
-
Gianmaria Fabrizio Ferrazzano, Giuseppe Di Fabio, Sara Caruso, Roberto Gatto, Varinder Goyal, and Silvia Caruso
- Subjects
dental injection ,lidocaine ,pain management ,pediatric dentistry ,prilocaine ,topical anesthetic ,Medicine - Abstract
Background: The injection of local anesthetics is the procedure that still causes the most fear and anxiety in a dental session; to minimize this problem, we can use topical anesthesia. The aim of this study is to analyze the tolerability and the clinical efficacy in the control of pain, during the subsequent injection of the local anesthetic, of an experimental anesthetic patch with a new formulation, which was previously tested in gel formula. Methods: A total of 150 children, aged 4 to 9 years, were included in the study. Each patient was treated using three different pre-anesthesia methods (placebo gel, experimental gel, and experimental patch), according to a split-mouth procedure, within a week of each other. The injection pain was analyzed using the WBFPRS and FLACC scales. Patients’ caregivers’ satisfaction was recorded at the end of the procedures. The data were analyzed using one-way ANOVA-RM, Wilcoxon–Mann–Whitney, Mann–Whitney U, and χ2 tests. Results: In this study, significantly higher pain ratings were observed with the topical placebo gel and lower pain ratings were observed with the experimental patch. Conclusion: The use of the patch proved to be very effective in reducing pain both subjectively and objectively, in the absence of both local and systemic side effects, validating its use in the oral mucosa.
- Published
- 2024
- Full Text
- View/download PDF
25. The Impact of Cytomegalovirus Infection on Natural Killer and CD8+ T Cell Phenotype in Multiple Sclerosis
- Author
-
Valentina Perri, Maria Antonella Zingaropoli, Patrizia Pasculli, Federica Ciccone, Matteo Tartaglia, Viola Baione, Leonardo Malimpensa, Gina Ferrazzano, Claudio Maria Mastroianni, Antonella Conte, and Maria Rosa Ciardi
- Subjects
cytomegalovirus ,multiple sclerosis ,NK cells ,CD8+ T cells ,NKT-like cells ,NKG2C ,Biology (General) ,QH301-705.5 - Abstract
Multiple sclerosis (MS) is a debilitating neurological disease that has been classified as an immune-mediated attack on myelin, the protective sheath of nerves. Some aspects of its pathogenesis are still unclear; nevertheless, it is generally established that viral infections influence the course of the disease. Cytomegalovirus (CMV) is a major pathogen involved in alterations of the immune system, including the expansion of highly differentiated cytotoxic CD8+ T cells and the accumulation of adaptive natural killer (NK) cells expressing high levels of the NKG2C receptor. In this study, we evaluated the impact of latent CMV infection on MS patients through the characterization of peripheral NK cells, CD8+ T cells, and NKT-like cells using flow cytometry. We evaluated the associations between immune cell profiles and clinical features such as MS duration and MS progression, evaluated using the Expanded Disability Status Scale (EDSS). We showed that NK cells, CD8+ T cells, and NKT-like cells had an altered phenotype in CMV-infected MS patients and displayed high levels of the NKG2C receptor. Moreover, in MS patients, increased NKG2C expression levels were found to be associated with higher EDSS scores. Overall, these results support the hypothesis that CMV infection imprints the immune system by modifying the phenotype and receptor repertoire of NK and CD8+ T cells, suggesting a detrimental role of CMV on MS progression.
- Published
- 2024
- Full Text
- View/download PDF
26. Atrioventricular nodal reentry tachycardia treatment using CARTO 3 V7 activation mapping: a new era of slow pathway radiofrequency ablation is under coming
- Author
-
Enrico Chieffo, Sabato D’Amore, Valentina De Regibus, Cinzia Dossena, Laura Frigerio, Erika Taravelli, Carolina Ferrazzano, Pasquale De Iuliis, Michele Cacucci, and Maurizio E. Landolina
- Subjects
slow pathway ,atrioventricular nodal reentry tachycardia ,RF ablation ,CARTO 3 version 7 ,activation map ,Confidense module ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
BackgroundSlow pathway (SP) ablation is the cornerstone for atrioventricular nodal reentry tachycardia (AVNRT) treatment, and a low-voltage bridge offers a good target during mapping using low x-ray exposure. We aimed to assess a new tool to identify SP by activation mapping using the last CARTO3® version, i.e., CARTO PRIME® V7 (Biosense Webster, Diamond Bar, CA, USA)Methods and resultsRight atrial septum and triangle of Koch 3D-activation map were obtained from intracardiac contact mapping during low x-ray CARTO 3® procedure. In 60 patients (mean age 60.3 ± 14.7, 61% females) undergoing ablation for AVNRT, an automatic activation map using a DECANAV® mapping catheter and CARTO® Confidense™, Coherent, and FAM DX software modules were obtained. The SP was identified in all patients as the latest atrioventricular node activation area; RF catheter ablation (RFCA) in that region elicited junctional beats. The mean procedural time was 150.3 ± 48.3 min, the mean fluoroscopy time exposure was 2.9 ± 2 min, the mean dose-area product (DAP) was 16.5 ± 2.7 cGy/cm2. The mean number of RF applications was 3.9 ± 2, the mean ablation index was 428.6 ± 96.6, and the mean contact force was 8 ± 2.8 g. There were no adverse event during the procedure, and no AVNRT recurrences occurred during a mean follow-up of 14.3 ± 8.3 months.ConclusionAblation of the SP by automatic mapping using Confidense™, Coherent, and FAM DX software modules is an innovative, safe, and effective approach to AVNRT ablation. The CARTO3® V7 system shows on a 3D map the latest AV node activation area during sinus rhythm allowing low fluoroscopy time and highly effective RFCA.
- Published
- 2023
- Full Text
- View/download PDF
27. Investigation of the GnRH antagonist degarelix isomerization in biological matrices
- Author
-
Lucia Ferrazzano, Alessandra Tolomelli, Ivan Guryanov, Marco Macis, Ulrich Abel, Antonio Ricci, and Walter Cabri
- Subjects
degarelix ,dihydroorotate ,hydantoin ,metabolism ,peptide ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Abstract One of the main objectives of peptide drug design is the improvement of peptide pharmacokinetics with maintaining biological activity, which can be achieved by the complex modifications of the primary structure of the peptides. However, these changes often lead to the formation of peculiar impurities in the peptide drugs and their metabolites, which require the development of advanced analytical methods to properly assess their content. Here, we investigated the degradation of the potent long‐acting GnRH antagonist degarelix in various biologic media by the tailor‐made HPLC method, which allows precise determination of 5‐Aph(Hyd)‐degarelix isomer, an impurity found in the degarelix active pharmaceutical ingredient (API) during its manufacturing. Unexpectedly, we discovered a rapid and irreversible conversion of degarelix API into the corresponding hydantoin isomer in serum, suggesting that this impurity can be also a potential drug metabolite in vivo. This finding underlines the importance of the development of more accurate and performing analytical techniques to correctly characterize the chemical composition of the manufactured drugs and their behavior under physiological conditions.
- Published
- 2023
- Full Text
- View/download PDF
28. Accurate Automatic Segmentation of Amygdala Subnuclei and Modeling of Uncertainty via Bayesian Fully Convolutional Neural Network
- Author
-
Liu, Yilin, Zhao, Gengyan, Nacewicz, Brendon M., Adluru, Nagesh, Kirk, Gregory R., Ferrazzano, Peter A, Styner, Martin, and Alexander, Andrew L.
- Subjects
Computer Science - Computer Vision and Pattern Recognition - Abstract
Recent advances in deep learning have improved the segmentation accuracy of subcortical brain structures, which would be useful in neuroimaging studies of many neurological disorders. However, most of the previous deep learning work does not investigate the specific difficulties that exist in segmenting extremely small but important brain regions such as the amygdala and its subregions. To tackle this challenging task, a novel 3D Bayesian fully convolutional neural network was developed to apply a dilated dualpathway approach that retains fine details and utilizes both local and more global contextual information to automatically segment the amygdala and its subregions at high precision. The proposed method provides insights on network design and sampling strategy that target segmentations of small 3D structures. In particular, this study confirms that a large context, enabled by a large field of view, is beneficial for segmenting small objects; furthermore, precise contextual information enabled by dilated convolutions allows for better boundary localization, which is critical for examining the morphology of the structure. In addition, it is demonstrated that the uncertainty information estimated from our network may be leveraged to identify atypicality in data. Our method was compared with two state-of-the-art deep learning models and a traditional multi-atlas approach, and exhibited excellent performance as measured both by Dice overlap as well as average symmetric surface distance. To the best of our knowledge, this work is the first deep learning-based approach that targets the subregions of the amygdala.
- Published
- 2019
29. Neural bases of motor fatigue in multiple sclerosis: A multimodal approach using neuromuscular assessment and TMS-EEG
- Author
-
Leodori, Giorgio, Mancuso, Marco, Maccarrone, Davide, Tartaglia, Matteo, Ianniello, Antonio, Certo, Francesco, Baione, Viola, Ferrazzano, Gina, Malimpensa, Leonardo, Belvisi, Daniele, Pozzilli, Carlo, Berardelli, Alfredo, and Conte, Antonella
- Published
- 2023
- Full Text
- View/download PDF
30. Exploring miRNAs’ Based Modeling Approach for Predicting PIRA in Multiple Sclerosis: A Comprehensive Analysis
- Author
-
Gosetti di Sturmeck, Tommaso, primary, Malimpensa, Leonardo, additional, Ferrazzano, Gina, additional, Belvisi, Daniele, additional, Leodori, Giorgio, additional, Lembo, Flaminia, additional, Brandi, Rossella, additional, Pascale, Esterina, additional, Cattaneo, Antonino, additional, Salvetti, Marco, additional, Conte, Antonella, additional, D’Onofrio, Mara, additional, and Arisi, Ivan, additional
- Published
- 2024
- Full Text
- View/download PDF
31. Efficacy of F-ACP-Containing Dental Mousse in the Remineralization of White Spot Lesions after Fixed Orthodontic Therapy: A Randomized Clinical Trial
- Author
-
Ciribè, Massimiliano, primary, Cirillo, Erika, additional, Mammone, Martina, additional, Vallogini, Giulia, additional, Festa, Paola, additional, Piga, Simone, additional, Ferrazzano, Gianmaria Fabrizio, additional, and Galeotti, Angela, additional
- Published
- 2024
- Full Text
- View/download PDF
32. Neural bases of motor fatigue in multiple sclerosis: A multimodal approach using neuromuscular assessment and TMS-EEG
- Author
-
Giorgio Leodori, Marco Mancuso, Davide Maccarrone, Matteo Tartaglia, Antonio Ianniello, Francesco Certo, Viola Baione, Gina Ferrazzano, Leonardo Malimpensa, Daniele Belvisi, Carlo Pozzilli, Alfredo Berardelli, and Antonella Conte
- Subjects
Neuromuscular assessment ,Motor cortex ,Corticospinal tract ,Transcranial magnetic stimulation-electroencephalography ,Motor fatigue ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Motor fatigue is one of the most common symptoms in multiple sclerosis (MS) patients. Previous studies suggested that increased motor fatigue in MS may arise at the central nervous system level. However, the mechanisms underlying central motor fatigue in MS are still unclear.This paper investigated whether central motor fatigue in MS reflects impaired corticospinal transmission or suboptimal primary motor cortex (M1) output (supraspinal fatigue). Furthermore, we sought to identify whether central motor fatigue is associated with abnormal M1 excitability and connectivity within the sensorimotor network.Twenty-two patients affected by relapsing-remitting MS and 15 healthy controls (HCs) performed repeated blocks of contraction at different percentages of maximal voluntary contraction with the right first dorsal interosseus muscle until exhaustion. Peripheral, central, and supraspinal components of motor fatigue were quantified by a neuromuscular assessment based on the superimposed twitch evoked by peripheral nerve and transcranial magnetic stimulation (TMS). Corticospinal transmission, excitability and inhibition during the task were tested by measurement of motor evoked potential (MEP) latency, amplitude, and cortical silent period (CSP). M1 excitability and connectivity was measured by TMS-evoked electroencephalography (EEG) potentials (TEPs) elicited by M1 stimulation before and after the task.Patients completed fewer blocks of contraction and showed higher values of central and supraspinal fatigue than HCs. We found no MEP or CSP differences between MS patients and HCs. Patients showed a post-fatigue increase in TEPs propagation from M1 to the rest of the cortex and in source-reconstructed activity within the sensorimotor network, in contrast to the reduction observed in HCs. Post-fatigue increase in source-reconstructed TEPs correlated with supraspinal fatigue values.To conclude, MS-related motor fatigue is caused by central mechanisms related explicitly to suboptimal M1 output rather than impaired corticospinal transmission. Furthermore, by adopting a TMS-EEG approach, we proved that suboptimal M1 output in MS patients is associated with abnormal task-related modulation of M1 connectivity within the sensorimotor network. Our findings shed new light on the central mechanisms of motor fatigue in MS by highlighting a possible role of abnormal sensorimotor network dynamics. These novel results may point to new therapeutical targets for fatigue in MS.
- Published
- 2023
- Full Text
- View/download PDF
33. Exploring the Central Mechanisms of Botulinum Toxin in Parkinson’s Disease: A Systematic Review from Animal Models to Human Evidence
- Author
-
Carolina Cutrona, Francesco Marchet, Matteo Costanzo, Maria Ilenia De Bartolo, Giorgio Leodori, Gina Ferrazzano, Antonella Conte, Giovanni Fabbrini, Alfredo Berardelli, and Daniele Belvisi
- Subjects
botulinum toxin ,Parkinson’s disease ,central nervous system ,animal models ,Medicine - Abstract
Botulinum toxin (BoNT) is an effective and safe therapy for the symptomatic treatment of several neurological disturbances. An important line of research has provided numerous pieces of evidence about the mechanisms of action of BoNT in the central nervous system, especially in the context of dystonia and spasticity. However, only a few studies focused on the possible central effects of BoNT in Parkinson’s disease (PD). We performed a systematic review to describe and discuss the evidence from studies focused on possible central effects of BoNT in PD animal models and PD patients. To this aim, a literature search in PubMed and SCOPUS was performed in May 2023. The records were screened according to title and abstract by two independent reviewers and relevant articles were selected for full-text review. Most of the papers highlighted by our review report that the intrastriatal administration of BoNT, through local anticholinergic action and the remodulation of striatal compensatory mechanisms secondary to dopaminergic denervation, induces an improvement in motor and non-motor symptoms in the absence of neuronal loss in animal models of PD. In human subjects, the data are scarce: a single neurophysiological study in tremulous PD patients found that the change in tremor severity after peripheral BoNT administration was associated with improved sensory–motor integration and intracortical inhibition measures. Further clinical, neurophysiological, and neuroimaging studies are necessary to clarify the possible central effects of BoNT in PD.
- Published
- 2023
- Full Text
- View/download PDF
34. Bioactive Peptides Derived from Edible Insects: Effects on Human Health and Possible Applications in Dentistry
- Author
-
Gianmaria Fabrizio Ferrazzano, Francesca D’Ambrosio, Sara Caruso, Roberto Gatto, and Silvia Caruso
- Subjects
edible insects ,nutrition ,bioactive peptides ,antihypertensive ,antioxidant ,anti-inflammatory ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Novel foods, including edible insects, are emerging because of their nutritional characteristics and low environmental impacts and could represent a valid alternative source of food in a more sustainable way. Edible insects have been shown to have beneficial effects on human health. Insect-derived bioactive peptides exert antihypertensive, antioxidant, anti-inflammatory, and antimicrobial properties and have protective effects against common metabolic conditions. In this review, the roles of edible insects in human health are reported, and the possible applications of these peptides in clinical practice are discussed. A special mention is given to the role of antimicrobial peptides and their potential applications in controlling infections in orthodontic procedures. In this context, insects’ antimicrobial peptides might represent a potential tool to face the onset of infective endocarditis, with a low chance to develop resistances, and could be manipulated and optimized to replace common antibiotics used in clinical practice so far. Although some safety concerns must be taken into consideration, and the isolation and production of insect-derived proteins are far from easy, edible insects represent an interesting source of peptides, with beneficial effects that may be, in the future, integrated into clinical and orthodontic practice.
- Published
- 2023
- Full Text
- View/download PDF
35. Fast Solution-Phase and Liquid-Phase Peptide Syntheses (SolPSS and LPPS) Mediated by Biomimetic Cyclic Propylphosphonic Anhydride (T3P®)
- Author
-
Alexia Mattellone, Dario Corbisiero, Paolo Cantelmi, Giulia Martelli, Chiara Palladino, Alessandra Tolomelli, Walter Cabri, and Lucia Ferrazzano
- Subjects
biomimetic reaction ,peptide synthesis ,homogeneous reaction ,SolPPS ,LPPS ,T3P® ,Organic chemistry ,QD241-441 - Abstract
The growing applications of peptide-based therapeutics require the development of efficient protocols from the perspective of an industrial scale-up. T3P® (cyclic propylphosphonic anhydride) promotes amidation in the solution-phase through a biomimetic approach, similar to the activation of carboxylic moiety catalyzed by ATP-grasp enzymes in metabolic pathways. The T3P® induced coupling reaction was applied in this study to the solution-phase peptide synthesis (SolPPS). Peptide bond formation occurred in a few minutes with high efficiency and no epimerization, generating water-soluble by-products, both using N-Boc or N-Fmoc amino acids. The optimized protocol, which was successfully applied to the iterative synthesis of a pentapeptide, also allowed for a decrease in the solvent volume, thus improving process sustainability. The protocol was finally extended to the liquid-phase peptide synthesis (LPPS), where the isolation of the peptide was performed using precipitation, thus also showing the suitability of this coupling reagent to this emerging technique.
- Published
- 2023
- Full Text
- View/download PDF
36. Mahalanobis distance tractometry (MaD-Tract) – a framework for personalized white matter anomaly detection applied to TBI
- Author
-
Guerrero-Gonzalez, Jose M, Yeske, Benjamin, Kirk, Gregory R, Bell, Michael J, Ferrazzano, Peter A, and Alexander, Andrew L
- Published
- 2022
- Full Text
- View/download PDF
37. Use of magnetic resonance imaging in severe pediatric traumatic brain injury: assessment of current practice.
- Author
-
Ferrazzano, Peter A, Rosario, Bedda L, Wisniewski, Stephen R, Shafi, Nadeem I, Siefkes, Heather M, Miles, Darryl K, Alexander, Andrew L, and Bell, Michael J
- Subjects
Clinical Research ,Neurosciences ,Childhood Injury ,Unintentional Childhood Injury ,Pediatric ,Biomedical Imaging ,Physical Injury - Accidents and Adverse Effects ,Brain Disorders ,Traumatic Brain Injury (TBI) ,Traumatic Head and Spine Injury ,Brain Injuries ,Traumatic ,Child ,Child ,Preschool ,Europe ,Female ,Glasgow Coma Scale ,Global Health ,Humans ,Image Processing ,Computer-Assisted ,Magnetic Resonance Imaging ,Male ,Outcome Assessment ,Health Care ,Time Factors ,United States ,magnetic resonance imaging ,pediatric traumatic brain injury ,survey ,trauma ,ACR = American College of Radiology ,ADAPT = Approaches and Decisions after Pediatric TBI ,DAI = diffuse axonal injury ,DTI = diffusion tensor imaging ,DWI = diffusion-weighted imaging ,GCS = Glasgow Coma Scale ,GOS-E Peds = Pediatric Glasgow Outcome Scale–Extended ,GRE = gradient recalled echo ,ICP = intracranial pressure ,MRS = MR spectroscopy ,PICU = pediatric intensive care unit ,PWI = perfusion-weighted imaging ,SWI = susceptibility-weighted imaging ,TBI = traumatic brain injury ,Paediatrics and Reproductive Medicine ,Neurology & Neurosurgery - Abstract
OBJECTIVE:There is no consensus on the optimal timing and specific brain MRI sequences in the evaluation and management of severe pediatric traumatic brain injury (TBI), and information on current practices is lacking. The authors performed a survey of MRI practices among sites participating in a multicenter study of severe pediatric TBI to provide information for designing future clinical trials using MRI to assess brain injury after severe pediatric TBI. METHODS:Information on current imaging practices and resources was collected from 27 institutions participating in the Approaches and Decisions after Pediatric TBI Trial. Multiple-choice questions addressed the percentage of patients with TBI who have MRI studies, timing of MRI, MRI sequences used to investigate TBI, as well as the magnetic field strength of MR scanners used at the participating institutions and use of standardized MRI protocols for imaging after severe pediatric TBI. RESULTS:Overall, the reported use of MRI in pediatric patients with severe TBI at participating sites was high, with 40% of sites indicating that they obtain MRI studies in > 95% of this patient population. Differences were observed in the frequency of MRI use between US and international sites, with the US sites obtaining MRI in a higher proportion of their pediatric patients with severe TBI (94% of US vs 44% of international sites reported MRI in at least 70% of patients with severe TBI). The reported timing and composition of MRI studies was highly variable across sites. Sixty percent of sites reported typically obtaining an MRI study within the first 7 days postinjury, with the remainder of responses distributed throughout the first 30-day postinjury period. Responses indicated that MRI sequences sensitive for diffuse axonal injury and ischemia are frequently obtained in patients with TBI, whereas perfusion imaging and spectroscopy techniques are less common. CONCLUSIONS:Results from this survey suggest that despite the lack of consensus or guidelines, MRI is commonly obtained during the acute clinical setting after severe pediatric TBI. The variation in MRI practices highlights the need for additional studies to determine the utility, optimal timing, and composition of clinical MRI studies after TBI. The information in this survey describes current clinical MRI practices in children with severe TBI and identifies important challenges and objectives that should be considered when designing future studies.
- Published
- 2019
38. Diagnosis and classification of blepharospasm: Recommendations based on empirical evidence
- Author
-
Kilic-Berkmen, Gamze, Defazio, Giovanni, Hallett, Mark, Berardelli, Alfredo, Ferrazzano, Gina, Belvisi, Daniele, Klein, Christine, Bäumer, Tobias, Weissbach, Anne, Perlmutter, Joel S., Feuerstein, Jeanne, and Jinnah, H.A.
- Published
- 2022
- Full Text
- View/download PDF
39. Neurophysiological and clinical biomarkers of secondary progressive multiple sclerosis: A cross-sectional study
- Author
-
Matteo Tartaglia, Marco Canevelli, Leonardo Malimpensa, Daniele Belvisi, Viola Baione, Gina Ferrazzano, Giorgio Leodori, Alfredo Berardelli, and Antonella Conte
- Subjects
multiple sclerosis ,frailty ,neurophysiology ,disease progression ,biomarkers ,transcranial magnetic stimulation ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Timely diagnosis of secondary progressive multiple sclerosis (SPMS) represents a clinical challenge. The Frailty Index, a quantitative frailty measure, and the Neurophysiological Index, a combined measure of sensorimotor cortex inhibitory mechanism parameters, have recently emerged as promising tools to support SPMS diagnosis. The aim of this study was to explore the possible relationship between these two indices in MS. MS participants underwent a clinical evaluation, Frailty Index administration, and neurophysiological assessment. Frailty and Neurophysiological Index scores were found to be higher in SPMS and correlated with each other, thus suggesting that they may capture similar SPMS-related pathophysiological mechanisms.
- Published
- 2023
- Full Text
- View/download PDF
40. Finding the source of motor fatigue in multiple sclerosis: a transcranial magnetic stimulation - electroencephalography study
- Author
-
Giorgio Leodori, Marco Mancuso, Davide Maccarrone, Matteo Tartaglia, Viola Baione, Antonio Ianniello, Gina Ferrazzano, Alfredo Berardelli, Carlo Pozzilli, and Antonella Conte
- Subjects
Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Published
- 2023
- Full Text
- View/download PDF
41. Identification of CX3CR1+ mononuclear phagocyte subsets involved in HIV-1 and SIV colorectal transmission
- Author
-
Cavarelli, Mariangela, Foglieni, Chiara, Hantour, Naima, Schorn, Tilo, Ferrazzano, Antonello, Dispinseri, Stefania, Desjardins, Delphine, Elmore, Ugo, Dereuddre-Bosquet, Nathalie, Scarlatti, Gabriella, and Le Grand, Roger
- Published
- 2022
- Full Text
- View/download PDF
42. Longitudinal evaluation of patients with isolated head tremor
- Author
-
Ferrazzano, Gina, Belvisi, Daniele, De Bartolo, Maria Ilenia, Baione, Viola, Costanzo, Matteo, Fabbrini, Giovanni, Defazio, Giovanni, Berardelli, Alfredo, and Conte, Antonella
- Published
- 2022
- Full Text
- View/download PDF
43. Validated Questionnaire on Intraoral Scanner Effectiveness in Paediatric Patients.
- Author
-
Caruso, Silvia, Di Benedetto, Giulia, Pelagalli, Pierluigi, Caruso, Luca, Caruso, Sara, Mattei, Antonella, Fiasca, Fabiana, Gatto, Roberto, Ferrazzano, Gianmaria Fabrizio, and Berdouses, Elias
- Subjects
INTRACLASS correlation ,CHILD patients ,CRONBACH'S alpha ,PEDIATRIC dentistry ,SCANNING systems ,DIGITAL communications - Abstract
The intraoral scanner represents an important innovation in the world of dentistry, capable of transforming and digitizing conventional workflows and significantly improving communication with the patient, especially in pediatric age. The aim of this study is to investigate how digital dentistry, using the intraoral scanner, can interact in the communicative trinomial dentist–pediatric patient–parents and how this tool can be useful in communicating and understanding the received message. The value of the introduction of digital dentistry on communication schemes is evaluated by administering a questionnaire of 17 questions. A sample of 100 children aged between 6 and 12 years was studied. Of the sample of children/parents included in the study, 50 were re-examined 4 weeks after the first visit to re-administer the questionnaire. Internal consistency, assessed through Cronbach's alpha, was adequate, being alpha equal to 0.728. Test–retest reliability was assessed with Cohen's kappa index for dichotomous variables (percentage of agreement was >85.71%), and for the discrete variables it was assessed with the Intraclass Correlation Coefficient (ICC). The reliability was moderate (0.5 < ICC < 0.75) for Q4, Q9, Q11 and Q1; for the other answers, the reliability was poor (ICC < 0.5) and not statistically significant. The digital approach is confirmed not only as a powerful innovation from an engineering or clinical point of view, but also as a useful tool for a more effective communication in the approach to pediatric patients. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
44. Does sex influence the natural history of idiopathic adult-onset dystonia?
- Author
-
Velucci, Vittorio, Idrissi, Sarah, Pellicciari, Roberta, Esposito, Marcello, Trinchillo, Assunta, Belvisi, Daniele, Fabbrini, Giovanni, Ferrazzano, Gina, Terranova, Carmen, Girlanda, Paolo, Majorana, Giovanni, Rizzo, Vincenzo, Bono, Francesco, Idone, Giovanni, Laterza, Vincenzo, Avanzino, Laura, Di Biasio, Francesca, Marchese, Roberta, Castagna, Anna, and Ramella, Marina
- Subjects
FOCAL dystonia ,HEPATOLENTICULAR degeneration ,PATIENT selection ,SLEEP interruptions ,SEX reversal ,NECK pain ,PREMATURE menopause - Published
- 2024
- Full Text
- View/download PDF
45. Post-discharge outcomes of hospitalized children diagnosed with acute SARS-CoV-2 or MIS-C.
- Author
-
Fink, Ericka L., Alcamo, Alicia M., Lovett, Marlina, Hartman, Mary, Williams, Cydni, Garcia, Angela, Rasmussen, Lindsey, Pal, Ria, Drury, Kurt, MackDiaz, Elizabeth, Ferrazzano, Peter A., Dervan, Leslie, Appavu, Brian, Snooks, Kellie, Stulce, Casey, Rubin, Pamela, Pate, Bianca, Toney, Nicole, Robertson, Courtney L., and Wainwright, Mark S.
- Published
- 2024
- Full Text
- View/download PDF
46. Functional motor phenotypes: to lump or to split?
- Author
-
Tinazzi, Michele, Geroin, Christian, Marcuzzo, Enrico, Cuoco, Sofia, Ceravolo, Roberto, Mazzucchi, Sonia, Pilotto, Andrea, Padovani, Alessandro, Romito, Luigi Michele, Eleopra, Roberto, Zappia, Mario, Nicoletti, Alessandra, Dallocchio, Carlo, Arbasino, Carla, Bono, Francesco, Magro, Giuseppe, Demartini, Benedetta, Gambini, Orsola, Modugno, Nicola, Olivola, Enrica, Bonanni, Laura, Zanolin, Elisabetta, Albanese, Alberto, Ferrazzano, Gina, De Micco, Rosa, Lopiano, Leonardo, Calandra-Buonaura, Giovanna, Petracca, Martina, Esposito, Marcello, Pisani, Antonio, Manganotti, Paolo, Tesolin, Lucia, Teatini, Francesco, Ercoli, Tommaso, Morgante, Francesca, and Erro, Roberto
- Published
- 2021
- Full Text
- View/download PDF
47. Early diagnosis of secondary progressive multiple sclerosis: focus on fluid and neurophysiological biomarkers
- Author
-
Ferrazzano, Gina, Crisafulli, Sebastiano Giuseppe, Baione, Viola, Tartaglia, Matteo, Cortese, Antonio, Frontoni, Marco, Altieri, Marta, Pauri, Flavia, Millefiorini, Enrico, and Conte, Antonella
- Published
- 2021
- Full Text
- View/download PDF
48. Data-driven clustering of combined Functional Motor Disorders based on the Italian registry
- Author
-
Giovanni Mostile, Christian Geroin, Roberto Erro, Antonina Luca, Enrico Marcuzzo, Paolo Barone, Roberto Ceravolo, Sonia Mazzucchi, Andrea Pilotto, Alessandro Padovani, Luigi Michele Romito, Roberto Eleopra, Carlo Dallocchio, Carla Arbasino, Francesco Bono, Pietro Antonio Bruno, Benedetta Demartini, Orsola Gambini, Nicola Modugno, Enrica Olivola, Laura Bonanni, Alberto Albanese, Gina Ferrazzano, Rosa De Micco, Maurizio Zibetti, Giovanna Calandra-Buonaura, Martina Petracca, Francesca Morgante, Marcello Esposito, Antonio Pisani, Paolo Manganotti, Fabrizio Stocchi, Mario Coletti Moja, Ilaria Antonella Di Vico, Lucia Tesolin, Francesco De Bertoldi, Tommaso Ercoli, Giovanni Defazio, Mario Zappia, Alessandra Nicoletti, and Michele Tinazzi
- Subjects
cluster analysis ,clinical phenotypes ,Functional Motor Disorders ,data-driven phenotyping ,functional neurological disorder ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
IntroductionFunctional Motor Disorders (FMDs) represent nosological entities with no clear phenotypic characterization, especially in patients with multiple (combined FMDs) motor manifestations. A data-driven approach using cluster analysis of clinical data has been proposed as an analytic method to obtain non-hierarchical unbiased classifications. The study aimed to identify clinical subtypes of combined FMDs using a data-driven approach to overcome possible limits related to “a priori” classifications and clinical overlapping.MethodsData were obtained by the Italian Registry of Functional Motor Disorders. Patients identified with multiple or “combined” FMDs by standardized clinical assessments were selected to be analyzed. Non-hierarchical cluster analysis was performed based on FMDs phenomenology. Multivariate analysis was then performed after adjustment for principal confounding variables.ResultsFrom a study population of n = 410 subjects with FMDs, we selected n = 188 subjects [women: 133 (70.7%); age: 47.9 ± 14.4 years; disease duration: 6.4 ± 7.7 years] presenting combined FMDs to be analyzed. Based on motor phenotype, two independent clusters were identified: Cluster C1 (n = 82; 43.6%) and Cluster C2 (n = 106; 56.4%). Cluster C1 was characterized by functional tremor plus parkinsonism as the main clinical phenotype. Cluster C2 mainly included subjects with functional weakness. Cluster C1 included older subjects suffering from anxiety who were more treated with botulinum toxin and antiepileptics. Cluster C2 included younger subjects referring to different associated symptoms, such as pain, headache, and visual disturbances, who were more treated with antidepressants.ConclusionUsing a data-driven approach of clinical data from the Italian registry, we differentiated clinical subtypes among combined FMDs to be validated by prospective studies.
- Published
- 2022
- Full Text
- View/download PDF
49. Mahalanobis distance tractometry (MaD-Tract) – a framework for personalized white matter anomaly detection applied to TBI
- Author
-
Jose M Guerrero-Gonzalez, Benjamin Yeske, Gregory R Kirk, Michael J Bell, Peter A Ferrazzano, and Andrew L Alexander
- Subjects
Multivariate ,Precision-medicine ,DTI ,TBI ,Tractometry ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Imaging-based quantitative measures from diffusion-weighted MRI (dMRI) offer the ability to non-invasively extract microscopic information from human brain tissues. Group-level comparisons of such measures represent an important approach to investigate abnormal brain conditions. These types of analyses are especially useful when the regions of abnormality spatially coincide across subjects. When this is not true, approaches for individualized analyses are necessary. Here we present a framework for single-subject multidimensional analysis based on the Mahalanobis distance. This is conducted along specific white matter pathways represented by tractography-derived streamline bundles. A definition for abnormality was constructed from Wilk's criterion, which accounts for normative sample size, number of features used in the Mahalanobis distance, and multiple comparisons. One example of a condition exhibiting high heterogeneity across subjects is traumatic brain injury (TBI). Using the Mahalanobis distance computed from the three eigenvalues of the diffusion tensor along the cingulum, uncinate, and parcellated corpus callosum tractograms, 8 severe TBI patients were individually compared to a normative sample of 49 healthy controls. For all TBI patients, the analyses showed statistically significant deviations from the normative data at one or multiple locations along the analyzed bundles. The detected anomalies were widespread across the analyzed tracts, consistent with the expected heterogeneity that is hallmark of TBI. Each of the controls subjects was also compared to the remaining 48 subjects in the control group in a leave-one-out fashion. Only two segments were identified as abnormal out of the entire analysis in the control group, thus the method also demonstrated good specificity.
- Published
- 2022
- Full Text
- View/download PDF
50. Clinical Efficacy and Tolerability of a New Experimental Mucoadhesive Patch for Topical Anesthesia of Oral Mucosa in Pediatric Dentistry
- Author
-
Ferrazzano, Gianmaria Fabrizio, primary, Di Fabio, Giuseppe, additional, Caruso, Sara, additional, Gatto, Roberto, additional, Goyal, Varinder, additional, and Caruso, Silvia, additional
- Published
- 2024
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.