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23 results on '"Ferraris, Laurent"'

1. Reduction of product composition variability using pooled microbiome ecosystem therapy and consequence in two infectious murine models

Author

Reygner, Julie, primary, Delannoy, Johanne, additional, Barba-Goudiaby, Marie-Thérèse, additional, Gasc, Cyrielle, additional, Levast, Benoît, additional, Gaschet, Enora, additional, Ferraris, Laurent, additional, Paul, Stéphane, additional, Kapel, Nathalie, additional, Waligora-Dupriet, Anne-Judith, additional, Barbut, Frederic, additional, Thomas, Muriel, additional, Schwintner, Carole, additional, Laperrousaz, Bastien, additional, and Corvaïa, Nathalie, additional

Published
2024
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4. Occurrence of Neonatal Necrotizing Enterocolitis in Premature Neonates and Gut Microbiota: A Case–Control Prospective Multicenter Study

Author

Aires, Julio, primary, Ilhan, Zehra Esra, additional, Nicolas, Lancelot, additional, Ferraris, Laurent, additional, Delannoy, Johanne, additional, Bredel, Maxime, additional, Chauvire-Drouard, Anne, additional, Barbut, Frédéric, additional, Rozé, Jean-Christophe, additional, Lepage, Patricia, additional, Butel, Marie-José, additional, and Group, ClosNEC Study, additional

Published
2023
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5. Clostridium neonatale antimicrobial susceptibility, genetic resistance determinants, and genotyping: a multicentre spatiotemporal retrospective analysis.

Author

Ferraris, Laurent, Delannoy, Johanne, Mazuet, Christelle, Diancourt, Laure, Mesa-Schein, Victoria, Barbut, Frédéric, and Aires, Julio

Subjects
*NEONATAL necrotizing enterocolitis, *MOBILE genetic elements, *CLOSTRIDIUM, *TRANSPOSONS, *CATECHOL-O-methyltransferase, *RETROSPECTIVE studies, *CEFOTAXIME
Abstract

Background Clostridium neonatale was isolated during an outbreak of neonatal necrotizing enterocolitis (NEC) in 2002. C. neonatale was validated as a new species within the genus Clostridium sensu stricto in 2018. In the present study, we evaluated the antimicrobial susceptibility, genetic determinants of resistance, and phylogenetic relationships of a collection of clinical isolates of C. neonatale. Methods C. neonatale strains (n = 68) were isolated from the stools of preterm neonates who either developed NEC or were asymptomatic carriers of C. neonatale in different periods and in different hospitals. Antimicrobial susceptibility was determined by the disc diffusion method. The MICs of clindamycin, cefotaxime and tetracycline were determined. Genetic determinants of resistance were screened by PCR (n = 68) and WGS (n = 35). Genotyping of the isolates was performed by MLST. Results Antimicrobial resistance was found to clindamycin (n = 24; 35%), cefotaxime (n = 7; 10%) and tetracycline (n = 1; 1%). One clindamycin-resistant isolate carried erm (B) by PCR. In addition, one isolate carrying tet (M) was tetracycline resistant (MIC = 16 mg/L) and 44 isolates carrying either tet (O), tet (32) or tet (M) were tetracycline susceptible (MICs < 16 mg/L). MLST showed that ST2 and ST15 were significantly associated with tet (32) (P < 0.0001) and tet (O) (P < 0.0001), respectively. From WGS, we identified aph(3′)-IIa and bla TEM-116 genes and a bla CBP-1-like gene. Conclusions C. neonatale is susceptible to anti-anaerobic molecules but resistant to clindamycin, cefotaxime and tetracycline. Genes encoding tetracycline ribosomal protection, macrolide-lincosamide-streptogramin B rRNA methyltransferase, aminoglycoside 3 '- phosphotransferase and β-lactamases have been identified in genomic regions flanked by mobile genetic elements. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Neonatal necrotizing enterocolitis: Clostridium butyricum and Clostridium neonatale fermentation metabolism and enteropathogenicity

Author

Ferraris, Laurent, primary, Balvay, Aurélie, additional, Bellet, Deborah, additional, Delannoy, Johanne, additional, Maudet, Claire, additional, Larcher, Thibaut, additional, Rozé, Jean-Christophe, additional, Philippe, Catherine, additional, Meylheuc, Thierry, additional, Butel, Marie-José, additional, Rabot, Sylvie, additional, and Aires, Julio, additional

Published
2023
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14. Neonatal necrotizing enterocolitis: Clostridium butyricumand Clostridium neonatalefermentation metabolism and enteropathogenicity

Author

Ferraris, Laurent, Balvay, Aurélie, Bellet, Deborah, Delannoy, Johanne, Maudet, Claire, Larcher, Thibaut, Rozé, Jean-Christophe, Philippe, Catherine, Meylheuc, Thierry, Butel, Marie-José, Rabot, Sylvie, and Aires, Julio

Abstract

ABSTRACTBacterial colonization in the gut plays a pivotal role in neonatal necrotizing enterocolitis (NEC) development, but the relationship between bacteria and NEC remains unclear. In this study, we aimed to elucidate whether bacterial butyrate end-fermentation metabolites participate in the development of NEC lesions and confirm the enteropathogenicity of Clostridium butyricumand Clostridium neonatalein NEC. First, we produced C.butyricumand C.neonatalestrains impaired in butyrate production by genetically inactivating the hbdgene encoding β-hydroxybutyryl-CoA dehydrogenase that produces end-fermentation metabolites. Second, we evaluated the enteropathogenicty of the hbd-knockout strains in a gnotobiotic quail model of NEC. The analyses showed that animals harboring these strains had significantly fewer and less intense intestinal lesions than those harboring the respective wild-type strains. In the absence of specific biological markers of NEC, the data provide original and new mechanistic insights into the disease pathophysiology, a necessary step for developing potential novel therapies.

Published
2023
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15. Antimicrobial susceptibility and resistance determinants of isolates from preterm infants

Author

Ferraris, Laurent, Butel, Marie-José, Aires, Julio, and Université Paris Descartes - Paris 5 (UPD5)

Subjects
Antibiotic resistance, polycyclic compounds, Preterm infants, biochemical phenomena, metabolism, and nutrition
Abstract

International audience; This study reports the antibiotic susceptibility and genetic resistance determinants of 39 strains isolated from the faeces of preterm infants as well as one reference strain. Results showed that all the strains were susceptible to cefoxitin, imipenem, vancomycin, tigecycline, metronidazole, chloramphenicol and linezolid. Resistance was observed to clindamycin (100%), penicillin G, amoxicillin and piperacillin (15%), tetracycline (7.5%) and erythromycin (5%). Investigation of the genetic basis of the observed resistance phenotypes showed that resistance to penicillin was due to β-lactamase activity and that resistance to tetracycline involved (O) or (O/32/O) homologue genes. Clindamycin and erythromycin resistance may involve another genetic determinant, different from those commonly described for clostridia.

Published
2010
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21. A fermented formula in pre-term infants: clinical tolerance, gut microbiota, down-regulation of faecal calprotectin and up-regulation of faecal secretory IgA

Author

Campeotto, Florence, primary, Suau, Antonia, additional, Kapel, Nathalie, additional, Magne, Fabien, additional, Viallon, Vivian, additional, Ferraris, Laurent, additional, Waligora-Dupriet, Anne-Judith, additional, Soulaines, Pascale, additional, Leroux, Bernard, additional, Kalach, Nicolas, additional, Dupont, Christophe, additional, and Butel, Marie-José, additional

Published
2011
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