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16 results on '"Ferrari, Elis Domingos"'

5. Cryptosporidium proventriculi in Captive Cockatiels (Nymphicus hollandicus)

Author

Panegossi, Mariele Fernanda da Cruz, primary, Widmer, Giovanni, additional, Nagata, Walter Bertequini, additional, Oliveira, Bruno César Miranda, additional, Ferrari, Elis Domingos, additional, Gomes, Jancarlo Ferreira, additional, Meireles, Marcelo Vasconcelos, additional, Nakamura, Alex Akira, additional, do Santos-Doni, Thaís Rabelo, additional, da Silveira Neto, Luiz, additional, and Bresciani, Katia Denise Saraiva, additional

Published
2023
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6. FIRST REPORT FROM Giardiaduodenalis PET RABBITS in Brazil

Author

Baptista, Carolina Beatriz, primary, Janeck Araújo, Matheus, additional, Inácio, Sandra Valéria, additional, Mendes, Bruno Criado de Araújo, additional, Aquino, Monally Conceição Costa de, additional, Ferrari, Elis Domingos, additional, Bresciani, Kátia Denise Saraiva, additional, and Costa, Alvimar José da, additional

Published
2023
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7. Efficacy of imidacloprid/flumethrin collar in preventing canine leishmaniosis in Brazil

Author

Alves, Graziella Borges, primary, de Oliveira, Talita Carolina Bragança, additional, Rodas, Lilian Colebrusco, additional, Rozza, Daniela Bernadete, additional, Nakamura, Alex Akira, additional, Ferrari, Elis Domingos, additional, da Silva, Debóra Regina Romualdo, additional, Santos, Gisele Moraes dos, additional, Calemes, Eliana Bravo, additional, Requena, Keuryn Alessandra Mira Luz, additional, Nagata, Walter Bertequini, additional, Santos‐Doni, Thais Rabelo, additional, and Bresciani, Katia Denise Saraiva, additional

Published
2022
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10. Detecção e caracterização molecular de Cryptosporidium spp. em canários mantidos em cativeiro (Serinus canaria) por diferentes métodos de diagnóstico

Author

Camargo, Vinícius da Silva, Santana, Bruna Nicoleti, Ferrari, Elis Domingos, Nakamura, Alex Akira, Nagata, Walter Bertequini, Nardi, Ana Rita Moraes, Meireles, Marcelo Vasconcelos, Universidade Estadual Paulista (Unesp), Faculdades Adamantinenses Integradas, and Fundação Municipal de Ensino Superior

Subjects
aves, diagnosis, birds, parasitic diseases, Cryptosporidiosis, epidemiology, epidemiologia, Criptosporidiose, diagnóstico
Abstract

This study used several diagnostic methods to examine the occurrence of and molecularly characterize Cryptosporidium spp. in captive canaries (Serinus canaria) in southern and southeastern Brazil. A total of 498 fecal samples were purified by centrifugal-flotation using Sheather's solution. Cryptosporidium spp. diagnosis was performed using three diagnostic methods: malachite green negative staining, nested PCR targeting the 18S rRNA gene, followed by sequencing the amplified fragments, and duplex real-time PCR targeting the 18S rRNA specific to detect Cryptosporidium galli and Cryptosporidium avian genotype III. The overall positivity for Cryptosporidium spp. (total samples positive in at least one protocol) from the microscopic analysis, nested PCR and duplex real-time PCR protocol results was 13.3% (66/498). The positivity rates were 2.0% (10/498) and 4.6% (23/498) for Cryptosporidium spp. by microscopy and nested PCR, respectively. Sequencing of 20 samples amplified by nested PCR identified C. galli (3.0%; 15/498), Cryptosporidium avian genotype I (0.8%; 4/498) and Cryptosporidium avium (0.2%; 1/498). Duplex real-time PCR revealed a positivity of 7.8% (39/498) for C. galli and 2.4% (12/498) for avian genotype III. Malachite green negative staining differed significantly from nested PCR in detecting Cryptosporidium spp. Duplex real-time PCR was more sensitive than nested PCR/sequencing for detecting gastric Cryptosporidium in canaries. Resumo Este trabalho teve como objetivos determinar a ocorrência e realizar a caracterização molecular de Cryptosporidium spp. em 498 amostras fecais de canários (Serinus canaria) criados em cativeiro, utilizando três métodos de diagnóstico: análise microscópica pela coloração negativa com verde malaquita, nested PCR seguida de sequenciamento dos fragmentos amplificados e PCR duplex em tempo real específica para detecção de Cryptosporidium galli e Cryptosporidium genótipo III de aves. A positividade total para Cryptosporidium spp. (total de amostras positivas em pelo menos um método de diagnóstico) obtida pela análise microscópica, nested PCR e PCR duplex em tempo real foi de 13,3% (66/498). As taxas de positividade para Cryptosporidium spp. foram 2,0% (10/498) e 4,6% (23/498) por microscopia e nested PCR, respectivamente. O sequenciamento de 20 amostras amplificadas pela nested PCR identificou C. galli (3,0%; 15/498), Cryptosporidium genótipo I de aves (0,8%; 4/498) e Cryptosporidium avium (0,2%; 1/498). A PCR duplex em tempo real revelou positividade de 7,8% (39/498) para C. galli e 2,4% (12/498) para Cryptosporidium genótipo III de aves. A análise microscópica diferiu significativamente da nested PCR para detecção de Cryptosporidium spp. A PCR duplex em tempo real apresentou maior sensibilidade que a nested PCR/sequenciamento para detectar as espécies/genótipos gástricos de Cryptosporidium.

Published
2018

11. Prevalence of Ehrlichia canis (Rickettsiales: Ehrlichieae) DNA in Tissues From Rhipicephalus sanguineus (Acari: Ixodidae) Ticks in Areas Endemic for Canine Monocytic Ehrlichiosis in Brazil

Author

Oliveira, Bruno César Miranda, primary, Ferrari, Elis Domingos, additional, Viol, Milena Araúz, additional, André, Marcos Rogério, additional, Machado, Rosangela Zacarias, additional, Costa de Aquino, Monally Conceição, additional, Inácio, Sandra Valéria, additional, Gomes, Jancarlo Ferreira, additional, Guerrero, Felix D, additional, and Bresciani, Katia Denise Saraiva, additional

Published
2018
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14. First description of Cryptosporidium parvum in carrier pigeons ( Columba livia )

Author

Oliveira, Bruno César Miranda, primary, Ferrari, Elis Domingos, additional, da Cruz Panegossi, Mariele Fernanda, additional, Nakamura, Alex Akira, additional, Corbucci, Flávio Sader, additional, Nagata, Walter Bertequini, additional, dos Santos, Bianca Martins, additional, Gomes, Jancarlo Ferreira, additional, Meireles, Marcelo Vasconcelos, additional, Widmer, Giovanni, additional, and Bresciani, Katia Denise Saraiva, additional

Published
2017
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15. First description of Giardia duodenalisin buffalo calves (Bubalus bubalis) in southwest region of São Paulo State, Brazil

Author

de Aquino, Monally Conceição Costa, Harvey, Tatiani Vitor, Inácio, Sandra Valéria, Nagata, Walter Bertequini, Ferrari, Elis Domingos, Oliveira, Bruno César Miranda, Albuquerque, George Rego, Widmer, Giovanni, Meireles, Marcelo Vasconcelos, and Bresciani, Katia Denise Saraiva

Abstract

We performed molecular characterization of Giardia duodenalisin buffalo calves from the Southwest region of São Paulo State, Brazil. A total of 183 fecal samples of Murrah breed buffaloes up to six months of age were collected. We examined these samples by the polymerase chain reaction (PCR) targeting the small-subunit ribosomal RNA gene and positive samples were characterized using additional PCR assays targeting a portion of the beta-giardin, the glutamate dehydrogenase and the triose-phosphate isomerase genes. Based on the SSUrRNA nPCR, the presence of G. duodenaliswas confirmed in 12 (6.56%) of fecal samples, of these, five, four and three samples were positive for the tpi, bgand gdhgenes, respectively. Assemblage identification by sequencing was successful in 6 of 12 samples and sequence analysis showed 100% genetic similarity with G. duodenalisassemblage E. This observation represents the first detection of G. duodenalisassemblage E in buffaloes calves in Brazil.

Published
2019
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16. Prevalence of Ehrlichia canis (Rickettsiales: Ehrlichieae) DNA in Tissues From Rhipicephalus sanguineus (Acari: Ixodidae) Ticks in Areas Endemic for Canine Monocytic Ehrlichiosis in Brazil.

Author

Oliveira BCM, Ferrari ED, Viol MA, André MR, Machado RZ, Costa de Aquino MC, Inácio SV, Gomes JF, Guerrero FD, and Bresciani KDS

Subjects
Animals, Brazil, DNA, Bacterial analysis, Female, Gastrointestinal Tract microbiology, Ovary microbiology, Salivary Glands microbiology, Arachnid Vectors microbiology, Ehrlichia canis isolation & purification, Rhipicephalus sanguineus microbiology
Abstract

Canine monocytic ehrlichiosis (CME) is a disease caused by the obligate intracellular bacterium Ehrlichia canis. Tropical lineages of Rhipicephalus sanguineus ticks play an essential role in the transmission of this pathogen. The aim of the present study was to evaluate the prevalence of E. canis DNA in tissue from R. sanguineus ticks in areas endemic for CME in Brazil and quantify levels of E. canis DNA in dissected tissues from these samples. A total of 720 ticks were collected from 72 dogs (36 dogs from the city Araçatuba in São Paulo state and 36 from Campo Grande in the state of Mato Grosso do Sul). Ticks were dissected to collect the guts, ovaries and salivary gland. A quantitative polymerase chain reaction (qPCR) targeting the disulphide bond formation (dsb) protein gene was performed to quantify the level of E. canis infection. The E. canis dsb-qPCR assay was positive for 31.9, 10, and 15.2% of the gut, ovary, and salivary glands, respectively. The average gut, ovary, and salivary gland bacterial load estimated by qPCR was 1.21 × 103, 2.60 × 103, and 4.92 × 103 gene copies/µl, respectively. This is the first report of E. canis DNA in ovaries of R. sanguineus ticks parasitizing dogs in these CME-endemic areas. These observations raise the possibility of E. canis trans-ovarial transmission., (© The Author(s) 2018. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Published
2019
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