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The prokineticin-2 (PROK2) is a small peptide belonging to the prokineticin family. In humans and rodents this chemokine is primarily involved in the control of central and peripheral reproductive processes. Klinefelter's syndrome (KS) is the first cause of male genetic infertility, due to an extra X chromosome, which may occur with a classical karyotype (47, XXY) or mosaic forms (46, XY/47, XXY). In affected subjects, pubertal maturation usually begins at an adequate chronological age, but when development is almost complete, they display a primary gonadal failure, with early spermatogenesis damage, and later onset of testosterone insufficiency. Thus, the main aim of the present study was to investigate the serum levels of PROK2 in prepubertal and adult KS patients, comparing them with healthy subjects. We showed for the first time the presence of PROK2 in the children serum but with significant changes in KS individuals. Indeed, compared with healthy subjects characterized by PROK2 serum elevation during the growth, KS individuals showed constant serum levels during the sexual maturation phase (higher during the prepubertal phase but lower during the adult age). In conclusion, these data indicate that in KS individuals PROK2 may be considered a biomarker for investigating the SK infertility process. Key words: Klinefelter syndrome, prepubertal, adults, prokineticin-2, infertility. La procineticine 2 (PROK2) est un petit peptide faisant partie de la famille des procineticines. Chez l'humain et les rongeurs, cette chimiokine joue principalement un role dans le controle central et peripherique des processus de la reproduction. Le syndrome de Klinefelter (SK) constitue la principale cause d'infertilite masculine de nature genetique en raison d'un chromosome X supplementaire, ce qui peut survenir avec un caryotype classique (47, XXY) ou des formes mosarques (46, XY/47, XXY). Chez les sujets atteints, le passage a la puberte commence habituellement a un age chronologique adequat, mais lorsque le developpement est presque termine, ils presentent une insuffisance gonadique primitive, avec des dommages de la spermatogenese precoces et une insuffisance de testosterone d'apparition plus tardive. Par consequent, la presente etude portait avant tout sur la comparaison des taux seriques de PROK2 entre des patients atteints de SK en prepuberte ou a l'age adulte et des sujets en bonne sante. Nous avons montre pour la premiere fois la presence de PROK2 dans le serum des enfants, mais avec des modifications importantes chez les personnes atteintes du SK. De fait, comparativement aux sujets en bonne sante caracterises par une hausse des taux seriques de PROK2 au cours de la croissance, les personnes atteintes du SK presentaient une augmentation des taux seriques de maniere constante au cours de la phase de maturation sexuelle (plus elevee au cours de la phase prepubere, mais moins elevee a l'age adulte). En conclusion, ces donnees montrent que l chez les personnes atteintes de SK, es taux de PROK2 pourraient etre consideres comme etant des biomarqueurs permettant d'etudier le processus d'infertilite dans le SK. [Traduit par la Redaction] Mots-cles: syndrome de Klinefelter, prepuberte, adultes, procineticine 2, infertilite., Introduction The chemokine prokineticin-2 (PROK2) is a small peptide belonging to the prokinetidn family. The preokineticins (PROK1 and PROK2) and their receptors (PKR1 and PKR2) are widely distributed in the [...]

Genetic features of alcohol dependence have been extensively investigated in recent years. A large body of studies has underlined the important role of genetic variants not only in metabolic pathways but also in the neurobiology of alcohol dependence, mediated by the neuronal circuits regulating reward and craving. Serotonin transporter (5-HTT), encoded by the SLC6A4 gene (Solute carrier family 6-neurotransmitter transporter-member 4), is targeted by antidepressant drugs such as selective serotonin reuptake inhibitors (SSRIs) and plays a pivotal role in serotoninergic transmission; it has been associated with psychiatric diseases and alcohol dependence. Transcriptional regulation and expression of 5-HTT depend not only on epigenetic modifications, among which DNA methylation (CpG and non-CpG) is primarily involved, but also on sequence variations occurring in intron/exon regions and in untranslated regions in 5′ and 3′, being the first sequences important for the splicing machinery and the last for the binding of transcription factors and micro RNAs. This work intends to shed light on the role of sequence variations known to affect the expression or function of 5-HTT in alcohol-dependent individuals. We found a statistically significant difference in the allelic (p = 0.0083) and genotypic (p = 0.0151) frequencies of the tri-allelic polymorphism, with higher function alleles and genotypes more represented in the control population. Furthermore, we identified three haplotypes more frequent in subjects with AUD (p < 0.0001) and one more frequent in the control population (p < 0.0001). The results obtained for the tri-allelic polymorphism in alcohol dependence confirm what is already present in part of the literature. The role of haplotypes requires further studies to be clarified. [ABSTRACT FROM AUTHOR]

Purpose: The aim of the study is to identify CT findings that are predictive of recurrence of acute uncomplicated colonic diverticulitis, to better risk-stratify these patients for whom guidelines recommend a conservative outpatient treatment and to determine the appropriate management with an improvement of health costs. Materials and Methods: Over the past year, 33 patients enrolled in an outpatient integrated care pathway (PDTA) for uncomplicated acute diverticulitis with 1-year follow-up period, without recurrence, and 33 patients referred to Emergency Department for a recurrent acute diverticulitis were included. Images of admission CT were reviewed by two radiologists and the imaging features were analyzed and compared with Chi-square and Student t tests. Univariate and multivariate Cox regression models were employed to identify parameters that significantly predicted recurrence in 1-year follow-up period and establish cutoff and recurrence-free rates. The maximally selected rank statistics (MSRS) were used to identify the optimal wall thickening cutoff for the prediction of recurrence. Results: Patients with recurrence showed a greater mean parietal thickness compared to the group without recurrence (16 mm vs. 11.5 mm; HR 1.25, p < 0.001) and more evidence of grade 4 of peridiverticular inflammation (40% vs. 12%, p = 0.009, HR 3.44). 12-month recurrence-free rates progressively decrease with increasing thickness and inflammation. In multivariate analysis, only parietal thickness maintained its predictive power with an optimal cutpoint > 15 mm that causes a sixfold increased risk of recurrence (HR 6.22; 95% CI, 3.05–12.67; p < 0.001). Beyond thickness and peridiverticular inflammation, predictive value of early recurrence within 90 days from the 1st episode resulted also an Hinchey Ib on admission CT. Conclusions: The maximum wall thickening and the grade of peridiverticular inflammation can be considered as predictive factors of recurrence and may be helpful in selecting patients for a tailored treatment to prevent the risk of recurrence. [ABSTRACT FROM AUTHOR]

Transthyretin-mediated amyloidosis (ATTR) is a systemic disease with protein precipitation in many tissues, mainly the peripheral nerve and heart. Both genetic (ATTRv, "v" for variant) and wild-type (ATTRwt) forms are known. Beyond the steric encumbrance, precipitated transthyretin seems to have a toxic effect. In this study carried out in men, we recruited 15 ATTRv patients, 7 ATTRv asymptomatic carriers, 14 ATTRwt patients and 10 young and 13 old healthy controls to evaluate the oxidative stress using FORD (Free Oxygen Radicals Defense) and FORT (Free Oxygen Radicals Test) analyses. ATTRv patients showed reduced FORD compared to ATTRwt and ATTRv asymptomatic carriers. FORD independently predicted the disease stage, with the early stages characterized by the highest consumption. These findings suggest a role for oxidative stress in the early stages of ATTRv. [ABSTRACT FROM AUTHOR]

This work highlights recent studies in epigenetic mechanisms that play a role in alcoholism, which is a complex multifactorial disorder. There is a large body of evidence showing that alcohol can modify gene expression through epigenetic processes, namely DNA methylation and nucleosomal remodeling via histone modifications. In that regard, chronic exposure to ethanol modifies DNA and histone methylation, histone acetylation, and microRNA expression. The alcohol-mediated chromatin remodeling in the brain promotes the transition from use to abuse and addiction. Unravelling the multiplex pattern of molecular modifications induced by ethanol could support the development of new therapies for alcoholism and drug addiction targeting epigenetic processes. Key words: epigenetics, alcohol metabolism, DNA methylation, histone modifications, chromatin remodeling. Ce travail met en lumiere les etudes recentes des mecanismes epigenetiques qui jouent un role dans l'alcoolisme, une maladie complexe multifactorielle. De nombreuses preuves ont montre que l'alcool peut modifier l'expression genique au moyen de processus epigenetiques, a savoir la methylation d'ADN et le remodelage nucleosomique par les modifications d'histones. Dans cette veine, l'exposition chronique a l'alcool modifie l'ADN et la methylation des histones, l'acetylation des histones et l'expression de microARN. Le remodelage de la chromatine medie par l'alcool dans le cerveau favorise la transition de l'usage vers l'abus et la dependance. La resolution du patron multiplexe des modifications moleculaires induites par l'alcool implique la capacite de developper de nouvelles therapies de l'alcoolisme et de la dependance aux drogues qui ciblent les processus epigenetiques. [Traduit par la Redaction] Mots-cles: epigenetique, metabolisme de l'alcool, methylation d'ADN, modifications d'histones, remodelage de la chromatine., Introduction Revealing the action of alcohol at the molecular level means a better understanding of the effects of alcohol on behavior (Ceccanti et al. 2015a, 2018a, 2018b; Coriale et al. [...]

Klinefelter syndrome (KS) is a male genetic disease caused by the presence of an extra X chromosome, causing endocrine disorders mainly responsible for a high rate of infertility and metabolic disorders in adulthood. Scientific research is interested in identifying new biomarkers that can be predictive or prognostic of alterations strictly connected to KS. Lipocalin-2 (LCN-2, also known as NGAL) is a small protein initially identified within neutrophils as a protein related to innate immunity. Serum LCN-2 estimation seems to be a useful tool in predicting the metabolic complications caused by several pathological conditions. However, little is known about its potential role in infertility conditions. The present pilot study aims to investigate the presence of LCN-2 in the serum of a group of pre-pubertal and post-pubertal children affected by KS, compared to healthy controls. We demonstrated for the first time the presence of elevated levels of LCN-2 in the serum of KS patients, compared to controls. This increase was accompanied, in pre-pubertal KS patients, by the loss of correlation with LH and HDL, which instead was present in the healthy individuals. Moreover, in all KS individuals, a positive correlation between LCN-2 and inhibin B serum concentration was found. Despite the limited size of the sample analyzed, our preliminary data encourage further studies to confirm the findings and to extend the study to KS adult patients, to verify the predictive/prognostic value of LCN-2 as new biomarker for metabolic diseases and infertility associated with the pathology. [ABSTRACT FROM AUTHOR]

Gender dysphoria is a clinical condition characterized by significant distress due to the discordance between biological sex and gender identity. Currently, gender dysphoria is also found more frequently in children and adolescents, thanks to greater social sensibleness and new therapeutic possibilities. In fact, it is estimated that the prevalence of gender dysphoria in pediatric age is between 0.5% and 2% based on the statistics of the various countries. Therefore, the pediatrician cannot fail to update himself on these issues and above all should be the reference figure in the management of these patients. Even if the patient must be directed to a referral center and be followed up by a multidisciplinary team, the treating pediatrician will care to coordinate the clinical and therapeutic framework. The aim of the present report is therefore to integrate literature data with our clinical experience to propose a new clinical approach in which the pediatrician should be the reference in the care of these patients, directing them towards the best therapeutic approach and staying in contact with the specialists of the referral center. [ABSTRACT FROM AUTHOR]