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Los virus papiloma humano (PVH) están presentes en la piel como flora normal, donde permanecen en forma latente, pudiendo desarrollar en ciertas oportunidades, lesiones cutáneas. Objetivo: Identificar los tipos de PVH con tropismo por epitelios cutáneos y analizar su posible asociación con lesiones cutáneas benignas y carcinomas cutáneos no melanoma. Materiales y métodos: Estudio prospectivo realizado en el servicio de dermatología del Hospital provincial del Centenario, desde Junio de 2007 a Noviembre de 2008. Se obtuvieron muestras mediante hisopados de regiones: fotoexpuesta (FE), no fotoexpuesta (NFE), perilesional (PL), superficie de lesión (SL) y biopsias de lesiones para estudio histopatológico. Se incluyeron pacientes derivados para estudio histopatológico de las lesiones antes referidas. Detección de PVH mediante PCR. Resultados: Participaron 67 pacientes, 41 hombres y 26 mujeres, edad promedio de 51 años (rango: 19-89 años). 300 muestras resultaron idóneas para la amplificación por PCR. La frecuencia de ADN de PVH hallado fue del 58% (176/300) (Figura 1), encontrándose 75% en FE, 39% en NFE, 75% en PL, 66% en SL y 35% en las biopsias. Se identificaron 69 tipos diferentes de HPV, siendo más frecuentes el 2, 21, 20 y 6. Se detectó un nuevo PVH, el 115. No se identificó PVH en muestras de carcinomas. En queratosis seborreicas se detectó en un 27%. Conclusiones: Se obtuvieron datos acerca de los PVH circulantes en los pacientes de nuestra región. Corroboramos la influencia de la radiación ultravioleta sobre la infección por este virus, así como su presencia en queratosis seborreicas. Identificamos un nuevo HPV en Sudamérica. The human papillomavirus (HPV) establish a latent infection of the skin as normal flora and can develop skin lesions on some situations. Objectives: Identify the HPV types which exhibit tropism for cutaneous epithelium and analyse its possible association with benign skin lesions and nonmelanoma skin cancer. Materials and methods: Prospective study was carried out in the Department of Dermatology at the Centenario Provincial Hospital from June 2007 to November 2008. Samples were collected by swabs from the following areas: sun-exposed (FE), non-sun-exposed (NFE), perilesional (PL), lesion area (SL) and biopsy of lesions for histopathological study. We included patients referred to surgical removal of a skin lesion for histopathological study. Detection of HPV by PCR. Results: 67 patients were recruited, 41 men and 26 women, median age of 51 years (range, 19-89 years). 300 samples were suitable for amplification by PCR. HPV DNA was present in 58% of the analyzed samples (176/300), and 75% in FE, 39% in NFE, 75% in PL, 66% in SL and 35% in biopsies. 69 different types of HPV were identified, being more frequent 2, 21, 20, and 6. We detected a new HPV, 115. HPV were not identified in samples of carcinomas. In seborrhoeic keratosis were present in 27% of the samples. Conclusions: We collected data about the HPV circulating in patients of our region. We affirmed the influence of ultraviolet radiation on this viral infection, as well as their presence in seborrhoeic keratosis. We identified a new HPV in South America. Fil: Piccirilli, Gustavo. Universidad Nacional de Rosario. Facultad de Cs.medicas; Argentina Fil: Squeff, M.. Universidad Nacional de Rosario. Facultad de Cs.medicas; Argentina Fil: Quattrocchi, C.. Universidad Nacional de Rosario. Facultad de Cs.medicas; Argentina Fil: Fernandez Bussy, Rodrigo. Universidad Nacional de Rosario. Facultad de Cs.medicas; Argentina Fil: Chouhy, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Gorosito, M.. Universidad Nacional de Rosario. Facultad de Cs.medicas; Argentina Fil: Sanchez, A.. Universidad Nacional de Rosario. Facultad de Cs.medicas; Argentina Fil: Bergero, A.. Universidad Nacional de Rosario. Facultad de Cs.medicas; Argentina Fil: Giri, Adriana Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Fernandez Bussy, R. A.. Universidad Nacional de Rosario. Facultad de Cs.medicas; Argentina

The purpose of the present study was to examine the phenotype of cutaneous immunocompetent cells and to quantify Langerhans cells in Lichen planus by the use of monoclonal antibodies directed against T-cell populations. Helper cells (OKT4+) and Suppressor/cytotoxic cells (OKT8+) were observed in all cutaneous infiltrates, and numerous Langerhans cells were identified by OKT6, BL6, and BL2 (HLA-DR) in the epidermis and dermis. The quantification of Langerhans cells demonstrated that the number of dendritic cells in epidermis is greater in involved skin than in non-involved skin. In recent lesions, Langerhans cells are more abundant than in older lesions. The results in peripheral blood indicated a T Helper/Suppressor imbalance with a decreased T-Suppressor/cytotoxic subpopulation in patients with lichen planus diseases. In lichen planus, our results suggest an immunological reaction involving all the immunocompetent cell subpopulations with a first stage of information by Langerhans cells (OKT6+, BL6+, BL2+ (HLA-DR)) and Helper cells (OKT4+), and second stage mediated by Suppressor/cytotoxic cells (OKT8+).

Retinoids are used in the treatment of lichen planus (LP) disease, but their mechanism of action remains unknown. We studied the epidermal Langerhans' cells in four patients with chronic LP, before and after treatment with aromatic retinoids. This study uses a new technique of quantification and exploits two monoclonal antibodies — OKT 6 specific for Langerhans' cells and BL 2 specific for HLA-DR antigens. Our results show an increase in the number of dendritic epidermal cells after treatment with oral retinoids. The number of OKT 6-positive cells is greater than the number of BL 2-positive (HLA-DR) cells before and after treatment.

The purpose of this study was to examine the phenotype of the cutaneous immunocompetent cells and Langerhans cells in malignant and benign tumour infiltrates (basal cell and squamous cell carcinomas, malignant melanoma, seborrheic keratosis and naevus) by the use of monoclonal antibodies directed against T cell populations and Langerhans cells. This in situ investigation indicates that the lymphocytes participating in the inflammatory reaction around skin tumours are mainly of the cytotoxic/suppressor class. It suggests, moreover, that interaction occurs in the skin between T lymphocytes and HLA-DR suppressing cells. The in situ study of the inflammatory immune response should be a useful complement to in vitro investigations in the exploration of immune reaction against tumours of the skin.

Summary Peripheral blood T lymphocytes and T cell subsets were examined in fifteen patients with lichen planus prior to and for 4 months during treatment. The percentages of different T cell sub-populations were defined by indirect immunofluorescence using monoclonal antibodies OKT3, OKT4 and OKT8. These are specific markers of total T cells, helper-inducer T cells and suppressor-cytotoxic T cells respectively. Decreased percentages of suppressor T cells and elevated helper suppressor ratios were observed before treatment and after 1 month of therapy. These changes had disappeared by the second month of treatment, by which time all the lesions had healed.