1. COI vs Ct: comparing automated antigen tests cut-off index (COI) to PCR cycle threshold (Ct).
- Author
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Urrutikoetxea-Gutierrez M, Liendo Arenzana P, Fernandez Vecilla D, Nieto Toboso MC, Ugalde Zarraga E, Macho Aizpurua M, and Diaz de Tuesta Del Arco JL
- Subjects
- Humans, SARS-CoV-2 genetics, Polymerase Chain Reaction, Immunologic Tests, Republic of Korea, Sensitivity and Specificity, COVID-19 diagnosis
- Abstract
Introduction: The objective of this study was to compare the numeric cut-off index (COI) of automated antigen tests with the cycle-threshold (Ct) value., Materials and Methods: COI values of all samples processed with the Elecsys® SARS-CoV-2 Antigen (Roche, Switzerland) from January to February 2022 were retrieved and the positive were compared to RdRP Cts of Allplex Variants I (Seegene, Korea). COI between 0.6 and 1 were considered indeterminate and overproved by RT-PCR., Results: From 13,937 samples 7944(57%) were positive and 189(1.35%) indeterminate. There was a strong correlation between Cts and COI values at the positive samples, but that was not the case for indeterminates., Conclusions: COI values of the positive samples (COI >1) are comparable to Ct values and may therefore be used as proxy viral loads, however this is not the case for indeterminate samples., Competing Interests: Declaration of competing interest The authors report no conflicts of interest relevant to this article., (Copyright © 2022 Elsevier Inc. All rights reserved.)
- Published
- 2023
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