Search

In follicular aspiration, physical aspects are of high significance for the technique to succeed, such as vacuum pressure, caliber of the needle and the way the follicular wall curettage is performed. The aim of this study was to investigate the recovery rate of equine oocytes aspirated by scraping of the follicular wall, testing different calibers of disposable needles, as well as the morphological evaluation of the cumulus oophorus complexes (COCs). Mares ovaries (n=447) obtained at a local slaughterhouse were transported to the laboratory in a thermal container (20 °C) and had the tunica albuginea and connective tissues dissected. The aspirated follicles had 10 to 25 mm in diameter, and 30x8 (21G 1 ¼) or 40x12 (18G 1 ½) needles were used for the aspiration, forming group A (G-A) and group B (G-B), respectively. In G-A and G-B, 480 and 548 follicles were aspirated, respectively. Under the stereomicroscope, the oocytes were evaluated according to the quality of the ooplasm and characteristics of the cumulus cells (grade I, II, III and denuded). The statistical analysis was performed using the Student’s t-test, logistic regression and test of proportions, and differences were considered significant when P

No intuito de comparar taxas de proliferação celular entre fêmeas Nelore (Bos indicus) com alta, média e baixa contagem de folículos antrais (CFA), ovários (n = 336) foram obtidos aos pares em abatedouro local e transportados até o laboratório. A classificação dos grupos a partir da contagem visual dos folículos, foi definida utilizando a média ± desvio padrão, em G-Alta (≥92 folículos), G-Média (46-76 folículos) e G-Baixa (≤ 31 folículos; p ≤ 0,05). A partir desta classificação, a atividade proliferativa folicular foi estabelecida em três grupos experimentais (alta n=5, média n=5 e baixa n=5). Estes ovários foram seccionados em fragmentos de 1 x 1 x 0,3cm, fixados em solução de formalina tamponada a 10%, processados e submetidas à avaliação imunohistoquímica para o anticorpo anti-PCNA. A imunoexpressão para PCNA (antígeno nuclear de proliferação celular) foi detectada em todos os estágios do desenvolvimento folicular. Foi encontrado o total de 1.688 folículos imunomarcados, dos quais 1.388 eram primordiais, 197 primários, 29 secundários e 74 antrais. Os dados de imunomarcação do PCNA de cada grupo foram submetidos ao teste de normalidade de resíduos (Shapiro-Wilk) e homocedasticidade (Levenne) e posteriormente comparados usando Teste de Fisher (p ≤ 0,05). Os grupos de G-Média e G-Baixa expressaram maior proliferação celular nos estágios finais do desenvolvimento folicular. Em contraponto, o grupo G-Alta obteve maior imunomarcação em folículos primordiais, com decréscimo até a fase de folículo antral. Os resultados obtidos através de avaliação imunohistoquímica sugerem que a polulação folicular antral, influencia na taxa de proliferação celular em fêmeas Nelore (Bos indicus). In order to compare cell proliferation rates between Nelore (Bos indicus) females with high, medium and low antral follicle count, ovaries (n = 336) were obtained in pairs at a local slaughterhouse and transported to the laboratory. The classification of groups was defined using mean ± standard error of the mean (SEM) into: G-High (≥ 92 follicles), G-Medium (46 – 76 follicles) and G-Low (≤ 31 follicles; p ≤ 0,05). After this evaluation the proliferative activity in the follicles was established into three experimental groups (high n=5, medium n=5 and low n=5). These ovaries were cut into fragments of 1 x 1 x 0,3cm, fixed in bufferedd formalina 10% solution, processed and subjected to immunohistochemical analysis for anti-PCNA antibody. The imune expression of PCNA was presente in all follicular development stages. Was found the amount of 1,688 immunomarkers follicles, of which 1,388 were primordial, 197 primary, 29 secondary and 74 antral. Immunostaining data for each animal underwent the waste normality test (Shapiro-Wilk) and homoscedasticity (Levenne) and then compared using Fisher's test. G-Medium and G-Low groups showed lower proliferation rate in early stages, and in the course of developing this rate increased progressively. On the other hand, the G-High group had greater cell proliferation immunostaining in primordial follicles, with decreased until the antral follicle stage. The results obtained through immunohistochemistry evaluation suggest that antral follicular population, influences in the cell proliferation rate in Nelore females (Bos indicus).

Due to the current understanding of infertility and absence of ovarian activity in the female mule, scientificmaterial regarding their reproductive tract is lacking. Information regarding evaluation of the reproductivesystem, as well as diagnoses of ovarian neoplasms, is scarce for female mules. The purpose of this paperis to report the first case of granulosa cell tumor (GCT) in a female mule, characterized through histology,which suggests evidence of cyclicity. During the breeding season, within the Southern hemisphere, mules’ovaries were obtained from slaughterhouses. Notably, a pair ovarian rated was remarkable in size (16.5cm in diameter), weight (1.92 kg) as well as number of antral follicles. On sagittal section, the tissue wastrabecular and fluid filled. Histology revealed irregular cyst formations full of amphophilic fluid ranging incolor from pinkish to purple. The granulosa cells showed up in abnormal histology, featuring a granulosacell tumor. The contralateral ovary was evaluated, demonstrating anatomy, weight and granulosa cellsfrom normal. Therefore, we have described the first granulosa cells tumor report in a mule via gross andhistological features, which suggests evidence of cyclicity in female mules.

The objectives of this study were to compare populations of preantral follicles between purebred Bos indicus and Bos taurus cows with high or low antral follicle counts (AFC) and to correlate the number of preantral follicles with the population of antral follicles. Nelore ( Bos indicus, n =100) and Angus ( Bos taurus, n =100) cow ovaries were collected at abattoirs and examined using ultrasonography. Antral follicles ≥3mm were counted, and the cows ovaries were assigned to high (G-High) or low (G-Low) AFC groups based on the mean number (±1 SD) of ovarian antral follicles: Bos indicus with high AFC (≥57 follicles, n =8) or low AFC (≤21 follicles, n =8) and Bos taurus with high (≥45 follicles, n =10) or low AFC (≤13 follicles, n =10). The ovaries were processed, and the number of preantral follicles was estimated. Between-groups comparisons were performed using a Kruskal-Wallis test, and the correlation between preantral and antral follicles was evaluated using a Pearson's correlation test ( P ≤0.05). A large variation in the number of preantral follicles was observed among the animals. Although there was a correlation between the population of preantral follicles and the number of antral follicles, there was no difference between the mean number of preantral follicles in the Bos indicus G-High (48,349±30,149) and G-Low groups (33,037±31,710) or between the Bos taurus G-High (35,050±36,060) and G-Low groups (30,481±43,360). Therefore, the preantral follicle population did not differ between purebred Bos indicus and Bos taurus cattle with high or low AFC but was correlated with the number of antral follicles. In addition to the large within-groups variation in the number of preantral follicles, some cows with high AFC had lower populations of preantral follicles compared to the low AFC group, and the highest population of preantral follicles was observed in both Bos indicus and Bos taurus with low AFC.