82 results on '"Fernanda Maria Policarpo Tonelli"'
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2. Contributors
- Author
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Shruti Agrawal, Shaban Ahmad, Prince Allawadhi, Sankarganesh Arunachalam, Anil Kumar Banothu, Shahanaz Begum, Kala Kumar Bharani, Srirupa Bhattacharyya, Trisha Biswas, Lakshita Chauhan, S Chockalingam, Helon Guimarães Cordeiro, Amrita Dam, Aradhana Dwivedi, Danilo Roberto Carvalho Ferreira, Deepa Garg, Mayank Goswami, Saravanan Govindaraj, Shikha Jain, Kajal Sandhu, Fatima Nazish Khan, Isha Khurana, Amit Khurana, Pramod Kumar, Selvaraj Kunjiappan, Anindita Laha, Moline Severino Lemos, Ishita Matai, Tarun Mateti, Sunita Mehta, Sankaranarayanan Murugesan, Ramavath Redya Naik, Dinesh Neeradi, Gopinath Packirisamy, Sureshbabu Ram Kumar Pandian, Theivendren Panneerselvam, Parasuraman Pavadai, Kitiporn Plaimas, Sahar Qazi, Muthuraman Raguraman, Mariappan Rajan, Ayyagari Ramlal, Vigneshwaran Ravishankar, Khalid Raza, Abhay Sachdev, Rajkumar Sadasivam, Kalpana Sagar, Deepanjali Sharma, Goutam Thakur, Sunitha Thalugula, Fernanda Maria Policarpo Tonelli, and Flávia Cristina Policarpo Tonelli
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- 2023
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3. Nanotechnology for point-of-care (POC) diagnostics
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Fernanda Maria Policarpo Tonelli, Moline Severino Lemos, Danilo Roberto Carvalho Ferreira, Flávia Cristina Policarpo Tonelli, and Helon Guimarães Cordeiro
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- 2023
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4. Biocompatibility of green synthesized nanomaterials
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Fernanda Maria Policarpo Tonelli and Flávia Cristina Policarpo Tonelli
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- 2023
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5. Algae-based green AgNPs, AuNPs, and FeNPs as potential nanoremediators
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Fernanda Maria Policarpo Tonelli, Christopher Santos Silva, Vinicius Marx Silva Delgado, and Flávia Cristina Policarpo Tonelli
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Fuel Technology ,Renewable Energy, Sustainability and the Environment ,Health, Toxicology and Mutagenesis ,General Chemical Engineering ,Environmental Chemistry ,Industrial and Manufacturing Engineering - Abstract
This review addresses green algae-based gold (Au), iron (Fe), and silver (Ag) nanoparticles (NPs) as eco-friendly nanomaterials to deal with biological, organic, and inorganic environmental contaminants. Among nanotechnological tools that can fully degrade, adsorb, and/or convert pollutants into less harmful structures, AgNPs, AuNPs, and FeNPs deserve highlight for their efficiency and low cost. However, green protocols are preferable to produce them in an eco-friendly manner. Although phycosynthesis is still in its infancy, algae present various advantages as green raw materials to NPs’ synthesis; fast growth rate, low-energy input requirement, low costs, easy and eco-friendly cultivation, and high tolerance to metals are examples. To allow their large-scale application, however, challenges regarding obtaining sufficient biomaterial with good reproducibility, designing protocols to achieve desirable features on NPs, and recovering the biocompatible nanomaterial after use still need attention. Perspectives for the field involve surpassing these limitations, broadening knowledge on synthesis mechanisms, protocols, and new species useful to offer, in the future, commercial eco-friendly, and low-cost phycosynthesized AuNPs, AgNPs, and FeNPs to nanoremediation. The potential of these NPs to deal with environmental contaminants, their advantageous characteristics and biocompatibility, the main limitations associated with their large-scale application, and future prospects for the field will receive attention.
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- 2023
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6. Exogenous application of phytohormones to increase plant performance under stress
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Fernanda Maria Policarpo Tonelli, Flávia Cristina Policarpo Tonelli, and Moline Severino Lemos
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- 2023
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7. Contributors
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Elif Esra Altuner, Wajiha Anum, Néstor Armendáriz-Alonso, Rezwana Assad, Samudrika Aththanayaka, Yıldız Aydın, Rizwana Ayub, Humera Aziz, Lubna Azmi, Mansoor Ahmad Bhat, Rouf Ahmad Bhat, Muhammad Bilal, Candy Carranza-Álvarez, Luis J. Castillo-Pérez, Sagarika Ekanayake, Vildan Erduran, Erum Feroz, Eftade O. Gaga, Kadir Gedik, Suresh Ghotekar, Itzel Alejandra Guevara-Ramírez, Mir Zahoor Gul, Younis Ahmad Hajam, Shahnawaz Hassan, Ghulam Hussain Jatoi, Rehana Kausar, Zulaykha Khurshid, Tariq Mehmood, Marta Michalska-Domańska, Rochak Mittal, Tapas Mukherjee, Mahwash Mukhtar, Balakrishnan Munirathinam, Ghulam Murtaza, Adnan Mustafa, Sana Nafees, Huda Nafees, S. Nizamudeen, Ruben del Olmo Martinez, Sampita Pal, Soumya Pandit, Tanveer Bilal Pirzadah, Muhammad Akram Qazi, Raksha Rani, Beedu Sashidhar Rao, Mashal Rehman, Umair Riaz, Arpita Roy, Karuna Rupula, Marium Saba, null Sabreena, Naila Safdar, Afifa Saghir, Ezgi Çabuk Şahin, Fatih Şen, Mohammad Ashraf Shah, Laila Shahzad, Richa Sharma, Preeti Sharma, D.V. Siva Reddy, Muhammad Irfan Sohail, Jayesh M. Sonawane, Gobika Thiripuranathar, Fernanda Maria Policarpo Tonelli, Flávia Cristina Policarpo Tonelli, Ashutosh Triphati, Ahu Altınkut Uncuoğlu, Güldem Utkan, Divya Virupannanavar, Azra Yasmin, Qamar Uz Zaman, Ali Zarrabi, and Mahira Zeeshan
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- 2023
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8. Contributors
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Chedly Abdelly, Arzoo Ahad, Madiha Ahmed, Kashif Akhtar, Eda Altinöz, Ergin Murat Altuner, Laiba Amin, Rabia Amir, Souhir Amraoui, Beenish Anjum, Augustine Antwi-Boasiako, Anoosha Arshad, Muhammad Ashraf, Habib-ur-Rehman Athar, Mustafeez Mujtaba Babar, Naheeda Begum, Rim Ben Youssef, Muhammad Faraz Bhatti, Nadia Boukari, Razmia Sabahat Butt, Zhicong Dai, Daolin Du, Hasna Ellouzi, Oushna Fajer, Zoha Fatima, Alvina Gul, Ghulam Haider, Hayet Houmani, Noor-ul- Huda, Mahnoor Ilyas, Yasemin İşlek, Cemil İşlek, Nahida Jelali, Attiya Rubab Khalid, Aziz Khan, Nabia Khan, Esra Koç, null Umm-E-Laila, Moline Severino Lemos, Noor Ul Ain Malik, Hamid Mohammadi, Faiza Munir, Nida Mushtaq, Maham Nadeem, Huda Nafees, Sana Nafees, Salman Nawaz, Misbah Naz, Mehr Un Nisa, S. Nizamudeen, Fadime Ozdemir-Kocak, Munir Ozturk, Hadi Pirasteh-Anosheh, Zuhra Qayyum, Mokded Rabhi, Marco Race, Bahareh Rahimpour, Gholamhassan Ranjbar, Rauoof Ahmad Rather, Muhammad Ammar Raza, Sahar Riaz, Muhammad Sajjad, Merve Şenturan, Gulcin Sevim, Muhammad Tariq, Fernanda Maria Policarpo Tonelli, Flávia Cristina Policarpo Tonelli, Huseyin Turker, Bengu Turkyilmaz Unal, Dilek Unal, Sania Zaib, Lixin Zhang, Tuanjie Zhao, Jianyu Zhou, and Walid Zorrig
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- 2023
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9. Microbes in Restoration of Polluted Ecosystems
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Fernanda Maria Policarpo Tonelli, Moline Severino Lemos, and Flávia Cristina Policarpo Tonelli
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- 2022
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10. Nanomaterials to Remediate Water Pollution
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Fernanda Maria Policarpo Tonelli, Flávia Cristina Policarpo Tonelli, Moline Severino Lemos, and Danilo Roberto Carvalho Ferreira
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- 2022
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11. Nanotechnology For Environmental Pollution Decontamination
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Fernanda Maria Policarpo Tonelli, Rouf Ahmad Bhat, and Gowhar Hamid Dar
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- 2022
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12. Contributors
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Ismail Abiola Adebayo, Azeez Omoniyi Adeoye, Adam Moyosore Afodun, Moniem B. Ahad, Kamoldeen Abiodun Ajijolakewu, Angel Josabad Alonso-Castro, Alice Amoding, Sagar Arya, Tabinda Athar, Muhammad Ashar Ayub, Arpita Banerjee, Monika Bansal, Twaha Ateenyi Basamba, Ashish Bedi, Isha Bedi, Sashidhar R. Beedu, Rouf Ahmad Bhat, Sasha Cardozo, Candy Carranza-Álvarez, Ramón Fernando García-De La Cruz, Gowhar Hamid Dar, Xiaorong Fan, Iqra Farooq, Zia Ur Rahman Farooqi, Nafeesa Farooq Khan, Saksham Garg, Muhammad Imran Ghani, Mir Z. Gul, Charu Gupta, Mahendra K. Gupta, Aukib Habib, Younis Ahmad Hajam, Khalid Rehman Hakeem, Insha Hameed, Humaira Hussain, Muhammad Mahroz Hussain, Predrag Ilic, Rashid Iqbal, Akanksha Jaiswar, Muatasim Jan, Rahul Singh Jasrotia, Rajdeep Jaswal, Manmeet Kaur, Navneet Kaur, Iqra F. Khan, Satya Prakash Khuntia, Md. Khursheed, Rajesh Kumar, Moline Severino Lemos, Mengyun Liu, Bisma Malik, Sweeta Manhas, Javid Manzoor, Tawseef A. Mir, Ghulam Murtaza, Umair Mustafa, Misbah Naz, Núbia Alexandre de Melo Nunes, Anjali Pathak, Tanveer Bilal Pirzadah, Amauri Ponce-Hernández, Paola Lucero Pérez, Ayesha Abdul Qadir, Mir S. Rabani, Shilpa Raina, Raksha Rani, Seema Rashid, Summia Rehman, Sobia Riaz, Umair Riaz, Arpita Roy, Jane Alexander Ruley, Karuna Rupula, Muhammad Fahad Sardar, Mohammad Sarraf, Riya Sharma, Shivani Sharma, Sheikh Mansoor, Harpreet Singh Sodhi, Hiralal Sonawane, Fernanda Maria Policarpo Tonelli, Flávia Cristina Policarpo Tonelli, Shivani Tripathi, John Baptist Tumuhairwe, Anjani Kumar Upadhyay, Bilal A. Wani, Khursheed Ahmad Wani, and Shabir H. Wani
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- 2022
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13. Conclusion and Future Perspectives
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Rouf Ahmad Bhat, Dig Vijay Singh, Fernanda Maria Policarpo Tonelli, and Khalid Rehman Hakeem
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- 2022
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14. Metabolic Routes to Biofuels Extraction
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Rouf Ahmad Bhat, Dig Vijay Singh, Fernanda Maria Policarpo Tonelli, and Khalid Rehman Hakeem
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- 2022
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15. Introduction: Values of Pollution-Free Atmosphere
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Rouf Ahmad Bhat, Dig Vijay Singh, Fernanda Maria Policarpo Tonelli, and Khalid Rehman Hakeem
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- 2022
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16. List of contributors
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Ismail Abiola Adebayo, Tariq Oluwakunmi Agbabiaka, Toyin Olayemi Agbabiaka, Ishtiyaq Ahmad, Sheikh Bilal Ahmad, Imtiaz Ahmed, Kamoldeen Abiodun Ajijolakewu, Md. Niamat Ali, Shafat Ali, Mohammad Yasir Arafat, Yahya Bakhtiyar, Abdul Baset, Pradyut Biswas, Monica Butnariu, Candy Carranza-Álvarez, Luis Jesús Castillo-Pérez, Tanmoy Gon Choudhary, Hilda Guadalupe Cisneros-Ontiveros, Núbia Alexandre de Melo Nunes, null Diksha, Mir Zahoor Gul, Shefali Gulliya, Shabana Gulzar, Younis Ahmad Hajam, Afrozah Hassan, Tasaduq Hussain Shah, Cesar Arturo Ilizaliturri-Hernandez, J. Immanuel Suresh, Swapnali Jadhav, Arizo Jan, Hanan Javid, Rajeev Kumar, Rajesh Kumar, Breno Luiz Sales Lemos, Moline Severino Lemos, Juan José Maldonado-Miranda, Junaid Malik, Nahum Andrés Medellin-Castillo, Maibam Malemngamba Meitei, Debashri Mondal, Sandip Mondal, Tamal Mondal, Irshad A. Nawchoo, Nighat Un Nissa, Bilal Ahmad Paray, Kapil Parihar, Amauri Ponce-Hernández, Raksha Rani, Muneeb U. Rehman, Andrea Guadalupe Rodríguez-López, Mahipal Singh Sankhla, Preeti Sharma, Podduturi Sindhura, Soibam Khogen Singh, Ngasotter Soibam, Swaroop S. Sonone, R. Sowndharya, M.S. Sri Janani, Uzma Sultana, Fernanda Maria Policarpo Tonelli, Flávia Cristina Policarpo Tonelli, Podduturi Vanamala, Tanaji Vasavilatha, Rohit Kumar Verma, Gusheinzed Waikhom, Aadil Farooq War, Leticia Guadalupe Yánez-Estrada, Ali Mohd Yatoo, Tabasum Yousuf, and Zarka Zaheen
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- 2022
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17. Mechanisms of phytoremediation
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Núbia Alexandre de Melo Nunes, Flávia Cristina Policarpo Tonelli, Fernanda Maria Policarpo Tonelli, and Moline Severino Lemos
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Phytoremediation ,business.industry ,Environmental science ,business ,Biotechnology - Published
- 2022
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18. Understanding the pathogenesis of important bacterial diseases of fish
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Fernanda Maria Policarpo Tonelli, Moline Severino Lemos, Flávia Cristina Policarpo Tonelli, Núbia Alexandre de Melo Nunes, and Breno Luiz Sales Lemos
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- 2022
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19. Plant and Algae Classes Recognition, Biomass Production and Potential Source of Biofuel
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Rouf Ahmad Bhat, Dig Vijay Singh, Fernanda Maria Policarpo Tonelli, and Khalid Rehman Hakeem
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- 2022
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20. Plant and Algae Metabolites Alternative and Clean Source of Energy
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Rouf Ahmad Bhat, Dig Vijay Singh, Fernanda Maria Policarpo Tonelli, and Khalid Rehman Hakeem
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- 2022
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21. Economic Consideration on Biofuel and Energy Security
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Rouf Ahmad Bhat, Dig Vijay Singh, Fernanda Maria Policarpo Tonelli, and Khalid Rehman Hakeem
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- 2022
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22. Plant and Algae Biomass
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Rouf Ahmad Bhat, Dig Vijay Singh, Fernanda Maria Policarpo Tonelli, and Khalid Rehman Hakeem
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- 2022
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23. The history of phytoremediation
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Gowhar Hamid Dar, Fernanda Maria Policarpo Tonelli, Rouf Ahmad Bhat, and Khalid Rehman Hakeem
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Pollutant ,Phytoremediation ,Balance (accounting) ,Environmental protection ,Environmental remediation ,Environmental science ,Ecosystem ,Environmental pollution - Abstract
As the environmental pollution is a major concern for human beings worldwide, different strategies have been developed over the years to try to mitigate the dangers associated with environmental pollutants and/or to efficiently remove them from contaminated areas, thus recovering the balance of the ecosystem. Phytoremediation is an efficient, well-accepted, low-cost, eco-friendly strategy to contribute to the restoration of environments. The purpose of this chapter is to review the historical development of plant-based remediation, highlighting the most relevant facts so that the scenario of today was achieved.
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- 2022
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24. Mechanism and Methods of Extraction of Biofuels
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Rouf Ahmad Bhat, Dig Vijay Singh, Fernanda Maria Policarpo Tonelli, and Khalid Rehman Hakeem
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- 2022
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25. Technical Challenges of Biofuel Obtainment
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Rouf Ahmad Bhat, Dig Vijay Singh, Fernanda Maria Policarpo Tonelli, and Khalid Rehman Hakeem
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- 2022
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26. Optimizations on Steps Involved on Biofuel Obtainment and their Validation
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Rouf Ahmad Bhat, Dig Vijay Singh, Fernanda Maria Policarpo Tonelli, and Khalid Rehman Hakeem
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- 2022
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27. Genetically Modified Organisms as Tools for Water Bioremediation
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Fernanda Maria Policarpo Tonelli, Flávia Cristina Policarpo Tonelli, and Moline Severino Lemos
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Bioremediation ,business.industry ,Biology ,business ,Biotechnology ,Genetically modified organism - Published
- 2021
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28. Nanotechnology-based Sensors for Detection of Environmental Pollution
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Fernanda Maria Policarpo Tonelli, Arpita Roy, Munir Ozturk, H C Ananda Murthy, Fernanda Maria Policarpo Tonelli, Arpita Roy, Munir Ozturk, and H C Ananda Murthy
- Abstract
Nanotechnology-based Sensors for Efficient Detection of Environmental Pollution discusses the use of nanotechnology to generate sensors capable of performing efficient detection of different types of environmental pollutants. Nanomaterial's characteristics such as large surface area, good reactivity, and possibility to suffer chemical surface modification to recognize different types of molecules are useful, especially to perform the detection of specific environmental pollutants. Innovative and efficient ways to detect environmental pollution are urgently needed for sustainability and the nanotechnology field has an enormous potential to offer strategic solutions. Nanotechnology-based sensors offer an efficient way of detecting the presence of contaminants and determine its structure and chemical nature is by applying nanotechnology and/or nanobiotechnology. This book will contain 5 parts: the first one will be dedicated to exploring environmental pollution as a threat to life on Earth and main contaminants (inorganic, organic or pathogens) and the risk they represent to living beings. The second part will be dedicated to nanotechnology allowing pollutants'detection covering a brief history of nanotechnology-based sensors, different types of nanotechnology-based sensor (optical, electrochemical, and magnetic), nanotechnology-based sensors'design and fabrication and nano biosensors. The third part will be focused on important specific pollutants (pesticides, heavy metal, dyes, toxic gas, pharmaceutical waste, petroleum hydrocarbons, and pathogenic microbes) and their detection by nanotechnology-based sensors. The fourth part will be dedicated to important nanomaterials in nanotechnology-based sensors, exploring carbon-based and non-carbon-based material in nanoscale (graphene, carbon nanotubes, quantum dots, magnetic nanomaterials, non-magnetic nanoparticles) and also point-of-care sensors and functionalization to generate optimized nanotechnology-based sensors to pollutants'detection. The fifth and last part of Nanotechnology-based Sensors for Efficient Detection of Environmental Pollution will address relevant practical aspects related to nanotechnology-based sensors, covering advantages and challenges, safety, economic and commercial aspects related to the field and also sustainability, highlighting green nanomaterials on nanotechnology-based sensors. - Provides a comprehensive, multidisciplinary review of nanotechnology-based sensors - Supplies readers extensive knowledge on detecting harmful pollutants in different environments using nanotechnology-based sensors - Presents chapters dedicated to the detection of pollutants different from toxic gas and pharmaceutical products, such as pesticides, heavy metals, dyes, pathogens, and petroleum hydrocarbons - Introduces information on pollutants and the threats they represent to living beings, nanotechnology-based sensor's design and fabrication, a brief history of the field, and practical issues related to the field, such as economics, safety, and challenges
- Published
- 2024
29. Nano-phytoremediation and Environmental Pollution : Strategies and Mechanisms
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Fernanda Maria Policarpo Tonelli, Rouf Ahmad Bhat, Gowhar Hamid Dar, Khalid Rehman Hakeem, Fernanda Maria Policarpo Tonelli, Rouf Ahmad Bhat, Gowhar Hamid Dar, and Khalid Rehman Hakeem
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- Phytoremediation, Environmental protection--Materials, Nanostructured materials--Industrial application
- Abstract
The book discusses nano-phytoremediation: the use of nanotechnology in combination with phytoremediation to restore polluted environs. The potentiality of plants in association with nanomaterials to effectively remediate polluted areas is elaborated meritoriously in this book. New strategies are necessary because anthropogenic actions represent a serious threat to life on Earth. This book has given enough space for a discussion of innovative and efficient technologies to restore damaged environs primarily focused on nano-phytoremediation. The first part of the book is dedicated to exploring organic and inorganic pollution and the threats they pose to living forms. The second part explores the joint use of plants and nanomaterials and the nano-phytoremediation of water and soil ecosystems. The book offers readers extensive knowledge on nano-phytoremediation as a feasible strategy to clean environmental pollution. The key features of the book are as follows: Nano-phytoremediation strategies to remediate soil and water ecosystems. Special chapters dedicated to different kinds of pollutants and methods of phytoremediation. Strategies to evaluate the success of nano-phytoremediation strategies, cost-effectiveness, and nano informatics to safe nanotechnology. The book can be used as a primary or supplementary text in undergraduate, graduate, and post-graduate courses such as biotechnology, biochemistry, and environmental engineering. It is an interesting edition for instructors, researchers, and scientists working on environmental management and pollution control.
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- 2024
30. Aquatic Contamination : Tolerance and Bioremediation
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Rouf Ahmad Bhat, Gowhar Hamid Dar, Fernanda Maria Policarpo Tonelli, Saima Hamid, Rouf Ahmad Bhat, Gowhar Hamid Dar, Fernanda Maria Policarpo Tonelli, and Saima Hamid
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- Water--Pollution, Bioremediation
- Abstract
Aquatic Contamination Authoritative resource presenting techniques and technologies to sustainably neutralize environmental contamination in aquatic plants, microorganisms, and more Two thirds of the Earth is covered with aquatic habitats that play a key role in stabilizing the global environment and providing a wide variety of services to increasing human needs. Nevertheless, anthropogenic activities are rapidly destroying the quality of both fresh and marine waters globally, due to excessive use of chemicals, fertilizers and pollution from suburban and industrial areas eventually making their way into the aquatic world. Aquatic Contamination: Tolerance and Bioremediation presents the broader spectrum of biological applicability of microbes with better understanding of cellular mechanisms for remediation of aquatic contaminants. The book also focuses on practices involved in molecular and genetic approaches, necessary to achieve targets of bioremediation and phytoremediation to solve global water contamination problems. Such approaches pave the way for the utilization of biological assets to design new, efficient, and environmentally sound remediation strategies by inculcating genomic techniques at cellular and molecular levels with model assessment. Aquatic Contamination provides a comprehensive background for readers interested in all perspectives of the contamination of aquatic environs. It covers various research aspects which are being carried out globally to understand simulation models in the assessment of xenobiotics, role of genomics, transgenic plants, and microbial enzymes for degradation and removal of toxic substances in aquatic environs. Key features include: Extensive coverage of interactions between plants, metals and microbes including the influence of biotic and abiotic factors Comprehensive discussion of the details of molecular mechanisms from assimilation to detoxification levels Exploration of the enzymatic approaches of potential plants acting as hyper-accumulators for contaminants in aquatic environs Details of sustainable tools such as transgenic plants for the manipulation of important functional microbial genes to achieve higher certainty of bioremediation Details of advances in tools and models like micro-arrays and simulation models for the complete assessment of xenobiotic compounds from cellular to degradation hierarchies Aquatic Contamination: Tolerance and Bioremediation will be substantially helpful to environmentalists, microbiologists, biotechnologists and scientists, providing essential information on various modern technologies for the remediation of contaminants in aquatic ecosystems.
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- 2023
31. Green Nanoremediation : Sustainable Management of Environmental Pollution
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Fernanda Maria Policarpo Tonelli, Arpita Roy, H C Ananda Murthy, Fernanda Maria Policarpo Tonelli, Arpita Roy, and H C Ananda Murthy
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- Bioremediation--Technological innovations, Nanotechnology
- Abstract
This book focuses on green nanoremediation addressing aspects related to the use of nanomaterials generated through green synthesis protocols to efficiently restore polluted environs. Nanomaterials'characteristics such as large surface area, capacity to easily reach into contaminated sites, good reactivity, and possibility of being developed to present photocatalytic activity and/or to deal with targeted substances by chemical surface modification are useful specially to perform remediation. As an alternative to conventional physicochemical methods, the green-based synthesis protocols reject the use of harmful reagents, prevent waste production, apply renewable energy source and/or materials, and consider in first place offering the smallest negative impact possible to living beings and to the ecosystem. Green synthesis in nanotechnology field involves the use of seaweeds, bacteria, cyanobacteria, yeasts, fungi, plants (living ones, biomass, extracts) and/or bio-derived products to generate the nanomaterials. The introductory chapter will be dedicated to nanomaterials'characteristics that enable them to be used in environmental remediation. The first part of the book will be dedicated to organic and inorganic pollution and the threats they pose to living forms; advantages, disadvantages and mechanisms of nanoremediation; comparison between conventional strategies of environmental pollution remediation and the green nanoremediation; carbon-based and non-carbon-based green nanomaterials capable of promoting environs'remediation; cost/benefits of using nanomaterials and nanoinformatics to a safe nanotechnology. The second part will be dedicated to green nanoremediation of water and soil, microbe-based, algae-based and plant-based synthesis of nanomaterials to nanoremediation. This part will also contain chapters dedicated to relevant nanomaterials for green nanoremediation protocols, nano-phytoremediation strategies, strategies to evaluate the efficiency of protocols related to this kind of remediation, main interactions of green nanomaterials and microbes during nanoremediation and, as a consequence of it, biocompatibility of green nanomaterials. This book's main purpose is to offer readers extensive knowledge on green nanoremediation as a feasible strategy to fight pollution's harmful consequences and clean environmental pollution, but also present the challenges that should be surpassed.
- Published
- 2023
32. Synthesis of Bionanomaterials for Biomedical Applications
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Munir Ozturk, Arpita Roy, Rouf Ahmad Bhat, Fazilet Vardar Sukan, Fernanda Maria Policarpo Tonelli, Munir Ozturk, Arpita Roy, Rouf Ahmad Bhat, Fazilet Vardar Sukan, and Fernanda Maria Policarpo Tonelli
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- Biomedical materials, Nanobiotechnology, Nanomedicine
- Abstract
Synthesis of Bionanomaterials for Biomedical Applications summarizes a range of procedures, including green synthesis of metal nanoparticles, metal oxide nanoparticles, and other types of nanoparticles while also exploring the appropriate use of these nanoparticles in various therapeutic applications such as anticancer, antibacterial, antifungal, drug delivery, and more. The book provides important information for materials scientists and pharmaceutical scientists on the synthesis of various nanoparticles using a variety of eco-friendly bionanomaterials. As concern has arisen regarding the environmental impact caused by some of nanomaterials, as well as their possible toxicity to cells, this book presents information on a new generation of eco-friendly materials. In addition, the green synthesis of nanoparticles shows how environmentally-friendly nanoparticles can be synthesized from different biological sources, such as microbes, fungi, algae and plants. - Provides information on the synthesis and application of eco-friendly bionanomaterials - Offers coverage of nanomaterials generated through green synthesis - Assesses the challenges of manufacturing eco-friendly nanomaterials on an industrial scale
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- 2023
33. Phytohormones and Stress Responsive Secondary Metabolites
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Munir Ozturk, Rouf Ahmad Bhat, Muhammad Ashraf, Fernanda Maria Policarpo Tonelli, Bengu Turkyilmaz Unal, Gowhar Hamid Dar, Munir Ozturk, Rouf Ahmad Bhat, Muhammad Ashraf, Fernanda Maria Policarpo Tonelli, Bengu Turkyilmaz Unal, and Gowhar Hamid Dar
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- Plant hormones, Plants--Effect of stress on, Plant metabolites
- Abstract
Phytohormones and Stress Responsive Secondary Metabolites provides a deep dive into the signaling pathways associated with phytohormones and phytometabolites. With a strong focus on plant stress responses and DNA technology, the book highlights plant biotechnology and metabolic engineering principles. Biotechnology, by using DNA editing technologies, allows the expression of plant genes into other plant species with desirable modulation on plant behavior. Beginning with an overview of phytohormone signaling, growth and abiotic and biotic stresses, subsequent chapters explore DNA modification strategies, epigenetic and epigenomic regulation, and miRNA regulation. This book will be an essential resource for students, researchers and agriculturalists interested in plant physiology, plant genetics and plant biotechnology. - Provides a comprehensive review of phytohormone and phytometabolite signaling pathways - Highlights recombinant DNA technology and therapeutic potential - Analyzes plant stress responses under both abiotic and biotic stresses
- Published
- 2023
34. Biocompatibility and Functionalization of Nanomaterials
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Danilo Roberto Carvalho Ferreira, Núbia Alexandre de Melo Nunes, Helon Guimarães Cordeiro, Karine Emanuelle da Silva, Flávia Cristina Policarpo Tonelli, Fernanda Maria Policarpo Tonelli, and Amanda Tomie Ouchida
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Biosafety ,Tissue engineering ,Biocompatibility ,Computer science ,Surface modification ,Nanotechnology ,Nanomaterials - Abstract
Nanomaterials can be designed, synthetized, and also functionalized to present key properties to be applied in biological and other kinds of systems. This chapter highlights some of the carbon-based and non-carbon-based nanomaterials, like their use to deliver different substances, imaging/diagnosis, tissue engineering, and energy storage. However, when it comes to their use in live systems it is necessary to check how safe it is to apply these materials, verifying if the desired effect will be achieved and if the action can be site-specific enough to guarantee biosafety. Therefore the chemical modification of nanomaterials is an important tool for its optimized and strategic use. Thus biocompatibility and functionalization of nanomaterials are also discussed in this chapter, which ends by presenting some future perspectives related to these relevant topics.
- Published
- 2020
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35. Approaches and Methods for Evaluation of Persistent Pollutant Load in Different Aquatic Environs
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Flávia Cristina Policarpo Tonelli and Fernanda Maria Policarpo Tonelli
- Subjects
Pollutant ,Water resources ,Environmental protection ,Environmental science ,Ecosystem ,Heavy metals ,Leaching (agriculture) ,Pesticide ,Surface water ,Groundwater - Abstract
Organic and inorganic persistent pollutants are a serious risk to human health and to other living forms, negatively affecting the ecosystem as a whole. Contaminated surface water and groundwater are a serious concern once there are limited water resources in the world and that not only quality standards of microbial risk that regulatory agencies were accustomed to watch over are enough to define water as secure to be used. Persistent chemicals (mainly pesticides and heavy metals), even in small amount, can represent an enormous risk to the forms of life in contact with the polluted water. Some human activities like industrial ones, when performed without environmental concern, can end up polluting especially water sources: directly or through leaching from soil. This chapter will focus on approaches and methods to evaluate persistent pollutant load in aquatic environs.
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- 2020
- Full Text
- View/download PDF
36. Concerns and Threats of Xenobiotics on Aquatic Ecosystems
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Fernanda Maria Policarpo Tonelli and Flávia Cristina Policarpo Tonelli
- Subjects
Pollutant ,Pollution ,Aquatic ecosystem ,media_common.quotation_subject ,Pesticide ,Environmental impact of pharmaceuticals and personal care products ,chemistry.chemical_compound ,chemistry ,Environmental protection ,Sustainability ,Environmental science ,Ecosystem ,Xenobiotic ,media_common - Abstract
Anthropogenic actions can result in environmental damage when they disrespect sustainability principles. The use of synthetic chemical substances as pesticides, the large presence of pharmaceuticals and personal care products in wastewaters, industrial activities using dyes or other organic compounds that can persist for a long time in nature are some examples of concerning sources of a group of pollutants called xenobiotics. This is a global problem that threatens the survival of various forms of life on the planet, including humans, and consequently from the ecosystem as a whole. This chapter will address specifically the concerns and threats related to the pollution of aquatic ecosystems by xenobiotics, highlighting the pesticides and pharmaceuticals.
- Published
- 2020
- Full Text
- View/download PDF
37. Pollution Remediation by Way of Using Genetically Modified Plants (GMPs)
- Author
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Fernanda Maria Policarpo Tonelli and Flávia Cristina Policarpo Tonelli
- Subjects
Pollutant ,Pollution ,Phytoremediation ,Pollution Remediation ,media_common.quotation_subject ,Chemical agents ,Environmental science ,Heavy metals ,Biochemical engineering ,Genetically modified crops ,Agricultural crops ,media_common - Abstract
As the process of man’s intervention on the environment is accelerated and enhances the range of industrial activities and the use of chemical agents to improve the yield of agricultural crops, pollution becomes an increasingly evident global problem that challenges the humanity and is difficult to solve. Not only human health is negatively affected but also all the ecosystem, involving other forms of life, soil, water, air, etc. In order to propose solutions to deal with heavy metals, explosives, industrial solvents, herbicides, and related pollutants, the recombinant DNA technology is a very important tool. It allows the development of transgenic organism able to eliminate the toxic residues from the environment. Transgenic plants deserve to be highlighted once their capacity to contribute can surpass the one offered by microorganisms in the uptake, transforming and limiting the toxicity of these contaminants being also a cost-effective strategy. This chapter aims to briefly review phytoremediation to remove, transform, or stabilize contaminants and its relevance to deal with persistent pollution capable of severely damaging life on earth.
- Published
- 2020
- Full Text
- View/download PDF
38. Causes and Effects of Pesticide and Metal Pollution on Different Ecosystems
- Author
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Flávia Cristina Policarpo Tonelli and Fernanda Maria Policarpo Tonelli
- Subjects
Pollution ,Sustainable development ,Pollutant ,Environmental protection ,media_common.quotation_subject ,Sustainability ,Environmental science ,Heavy metals ,Ecosystem ,Pesticide ,Metal pollution ,humanities ,media_common - Abstract
Due to anthropogenic interference in the environment disrespecting the principles of sustainability, pollution is currently a serious matter of concern. This increasingly global problem threatens the survival not only of humans but also of other life forms on the planet as contaminants pose serious risks to health and to the ecosystem as a whole. This chapter will address the main causes and consequences of pollution due to two highly harmful pollutants that require attention: pesticides and heavy metals.
- Published
- 2020
- Full Text
- View/download PDF
39. Role of Modern Innovative Techniques for Assessing and Monitoring Heavy Metal and Pesticide Pollution in Different Environments
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Flávia Cristina Policarpo Tonelli and Fernanda Maria Policarpo Tonelli
- Subjects
Pollutant ,Pollution ,Bioremediation ,Waste management ,media_common.quotation_subject ,Environmental science ,Heavy metals ,Pesticide pollution ,Pesticide ,media_common - Abstract
Pollution from heavy metals and pesticides is a major problem as these pollutants can cause irreparable damage to health or end the life of living beings exposed to them. Therefore, it is extremely important not only to create strategies to avoid these and other types of pollution and to remediate contaminated areas. It is necessary to develop innovative techniques for assessing and monitoring these pollutants in different types of samples and it is desirable that these new methodologies offer advantages over the methods conventionally used for these purposes. In this chapter, these types of pollution will be addressed and innovative ways of detecting these pollutants in different kinds of samples will be emphasized.
- Published
- 2020
- Full Text
- View/download PDF
40. List of Contributors
- Author
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Jyoti Ahlawat, Kanwal Akhtar, Rakhi Chaudhary, Helon Guimarães Cordeiro, Núbia Alexandre de Melo Nunes, Deepanjalee Dutta, Karine Emanuelle da Silva, Danilo Roberto Carvalho Ferreira, Deepa Garg, Upasana Gupta, Marlia Mohd Hanafiah, Jaidip M. Jagtap, Yasir Javed, Mohammad Nadeem Khan, Vinay Kumar, Sivarama Krishna Lakkaboyana, Subhrangsu Sunder Maitra, Ishita Matai, Mukesh Kumar Meher, Mahesh Narayan, Amanda Tomie Ouchida, Abdul K. Parchur, Krishna Mohan Poluri, Abhay Sachdev, Sunil Kumar Sailapu, Naveed Akhtar Shad, Gayatri Sharma, Neha Sharma, Barkha Singhal, Fernanda Maria Policarpo Tonelli, and Flávia Cristina Policarpo Tonelli
- Published
- 2020
- Full Text
- View/download PDF
41. Mechanisms and Importance of Phytoremediation
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Moline Severino Lemos, Núbia Alexandre de Melo Nunes, Fernanda Maria Policarpo Tonelli, and Flávia Cristina Policarpo Tonelli
- Subjects
Pollutant ,Phytoremediation ,Environmental remediation ,Rhizofiltration ,Plant species ,Environmental science ,Environmental pollution ,Environmental planning - Abstract
Environmental pollution threatens life existence on Earth. It is necessary and urgent that human beings develop efficient and safe strategies to remediate the harmful impacts caused by anthropogenic action over ecosystems. The use of plants is an interesting tool to promote environs’ restoration through phytoremediation. This kind of remediation can be performed by different mechanisms depending on the type of pollutant to be remediated, if the target environment is aqueous or soil, and on the plant species chosen. This chapter will focus on phytoremediation mechanisms and their importance as a strategic method of dealing with the restoration of polluted environs: an essential step toward achieving sustained development respecting the environment and developing environmental awareness.
- Published
- 2020
- Full Text
- View/download PDF
42. Application of Biotechnology for Restoration of Degraded Environs
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Flávia Cristina Policarpo Tonelli, Fernanda Maria Policarpo Tonelli, and Helon Guimarães Cordeiro
- Subjects
Pollutant ,Environmental remediation ,Large array ,Acute intoxication ,Environmental science ,Environmental planning - Abstract
Pollution represents a serious risk to living forms once it can cause from acute intoxication to death. In order to avoid the damage or restrict it, if it is not possible to eliminate it, it is necessary to develop remediation strategies. These different strategies offer a large array of options available to be used in an optimized way to remediate different kinds of environs and of pollutant or pollutants (organic and/or inorganic) present. Remediation protocols can use living organisms (biological remediation) or not (physicochemical remediation). The former group is the focus of this chapter that explores biotechnologies to restore contaminated environs and contribute to the achievement of sustained development. The use of plants, microorganisms, biochars, nanomaterials, and also the genetic manipulation of living beings are discussed as tools to develop efficient and safe protocols to try to repair the damages anthropic action caused and still causes over ecosystems.
- Published
- 2020
- Full Text
- View/download PDF
43. Nanotechnology for Environmental Pollution Decontamination : Tools, Methods, and Approaches for Detection and Remediation
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Fernanda Maria Policarpo Tonelli, Rouf Ahmad Bhat, Gowhar Hamid Dar, Fernanda Maria Policarpo Tonelli, Rouf Ahmad Bhat, and Gowhar Hamid Dar
- Subjects
- Detectors, Pollutants, Nanotechnology--Environmental aspects
- Abstract
This new volume presents informative research on the different aspects of employing nanotechnology for environmental pollution decontamination, highlighting the main tools, methods, and approaches for contaminants detection and remediation. The book takes a biotechnological point of view that considers the main environmental pollutants; the safety and economic aspects of nanoremediation, nanosensors and nanobiosensors for the detection of pollutants; and strategies to promote nanoremediation and nanobioremediation. The chapters offer a comprehensive overview of nanotechnologic strategies as essential tools to restore polluted environments and to make more feasible and harmonic the pathway to sustainable development. The volume also discusses the use of sensors to detect pollutants and to monitor the quality of environmental restoration. Topics include nanozymes; organic and inorganic pollutants threatening human health; different types of carbon-based and non-carbon-based nanomaterials in nanosensors and nanobiosensors to detect environmental pollution; nanomaterials that specifically deal with water, soil, or air pollution; and assisted nanoremediation promoted by plants (nanophytoremediation) or microorganisms (for example, mycorrhizal fungus) that promote in situ nano-phyto-mycorrhizo-remediation. Also addressed are aspects related to a macroperspective of nanoremediation that highlight the economic aspects related to nanotechnology, the safety aspects of the use of nanomaterials, and the sustainability aspects related to the use of nanomaterials in strategies of environmental restoration. Nanotechnology for Environmental Pollution Decontamination: Tools, Methods, and Approaches for Detection and Remediation offers extensive and comprehensive knowledge on nanotechnology applied to pollution detection and remediation, assisted or not by biological strategies.
- Published
- 2022
44. Plant and Algae Biomass : Feasible Sources for Biofuel Production
- Author
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Rouf Ahmad Bhat, Dig Vijay Singh, Fernanda Maria Policarpo Tonelli, Khalid Rehman Hakeem, Rouf Ahmad Bhat, Dig Vijay Singh, Fernanda Maria Policarpo Tonelli, and Khalid Rehman Hakeem
- Subjects
- Botany, Environment, Biotechnology, Renewable energy sources, Cogeneration of electric power and heat, Fossil fuels
- Abstract
This volume discusses how plant and algae organisms play a pivotal role in the transformation of solar energy to essential metabolites, and explores the numerous beneficial roles these metabolites have at an industrial level. It presents information on the utilization of plant and algae for biomass production, and shows how this is a practical option for large scale biofuel production. The book examines how these bio-metabolites can then be used to extract biofuel. Biomass produced from plants and algae can act as the source of feedstock for biofuel production and industrially important compounds. This book also explores that by curtailing culturing cost using wastewater, seawater, and industrial water as a nutrient and water source, biomass becomes an economical energy source. The introductory chapters of the book focus on the appreciative values of a pollution-free atmosphere, with special reference to enhanced greenhouse effect, and then are followed by chapters on the potential of plant and algae as a liquid energy resource. This book targets researchers, graduate students, and energy and fuel industry professionals interested in the plant sciences, biotechnology and renewable energy.
- Published
- 2022
45. Phytoremediation : Biotechnological Strategies for Promoting Invigorating Environs
- Author
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Rouf Ahmad Bhat, Fernanda Maria Policarpo Tonelli, Gowhar Hamid Dar, Khalid Rehman Hakeem, Rouf Ahmad Bhat, Fernanda Maria Policarpo Tonelli, Gowhar Hamid Dar, and Khalid Rehman Hakeem
- Abstract
Phytoremediation: Biotechnological Strategies for Promoting Invigorating Environs focuses on phytoremediation's history, present and future potential, discussing mechanisms of remediation, different types of pollutant and polluted environs, cell signaling, biotechnology, and molecular biology, including site-directed DNA and the omics related to plant sciences. Sections focus on phytoremediation as an economically feasible and environmentally safe strategy, including its mechanisms from macroscopic to microscopic level, strategies of assisted phytoremediation, the role of omics on innovations on the field, the development of genetically modified plants (GMPs) to deal with pollutants, the future prospects of targeted genetic engineering in phytoremediation and remediation advantages and disadvantages. Other sections in the book explore the phytoremediation of specific environs (water and soil) and specific contaminants that are of major worldwide concern. - Presents phytoremediation mechanisms at a microscopic level (molecular mechanisms) - Covers remediation in different environs and in different kinds of pollutants - Conveys the economic aspects relating to phytoremediation
- Published
- 2021
46. Studying complex system: calcium oscillations as attractor of cell differentiation
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Alexandre Hiroaki Kihara, Henning Ulrich, Mauro Cunha Xavier Pinto, Fernanda Maria Policarpo Tonelli, Rodrigo R. Resende, and André L. G. Vieira
- Subjects
0301 basic medicine ,Neurogenesis ,Cellular differentiation ,Biophysics ,Biology ,Biochemistry ,03 medical and health sciences ,Oscillometry ,Calcium flux ,Animals ,Homeostasis ,Humans ,Calcium Signaling ,Transcription factor ,Calcium signaling ,Neurons ,Chemotaxis ,Stem Cells ,Cell Differentiation ,Models, Theoretical ,CÁLCIO ,Cell biology ,Phenotype ,030104 developmental biology ,Neuron differentiation ,Calcium ,Signal transduction ,Neural development ,Signal Transduction - Abstract
Biological processes, such as the induction of undifferentiated cells to enable neurogenesis, provide complex mechanisms for study. For further insight, subsets of these processes that are governed by metabolic pathways or key molecules called attractors need to be elucidated. In this review, we have focused on the role of calcium as a driving force of neuronal differentiation. The activity of calcium refers to peaks and waves, whose amplitudes and frequencies in stem and progenitor cells involve the activation of a great variety of signaling pathways that comprise neurotransmitters and their receptors, intracellular signaling factors and transcription factors, which form a complex network. The study of different subsets, from receptor-mediated calcium flux to the activation of transcription factors, can then be combined to understand the process of neuronal differentiation.
- Published
- 2016
- Full Text
- View/download PDF
47. Efficient and safe gene transfection in fish spermatogonial stem cells using nanomaterials
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C. A. Furtado, R. R. Resende, S. M. S. N. Lacerda, M. S. Lemos, Fernanda Maria Policarpo Tonelli, A. C. de Jesus, Nicole de Cássia Oliveira Paiva, Luiz R. França, F. G. Pacheco, Luiz Orlando Ladeira, and J. D. Corrêa-Junior
- Subjects
0301 basic medicine ,Programmed cell death ,biology ,General Chemical Engineering ,Electroporation ,food and beverages ,General Chemistry ,Transfection ,Gene delivery ,biology.organism_classification ,Molecular biology ,Cell biology ,Nanomaterials ,Transgenesis ,03 medical and health sciences ,chemistry.chemical_compound ,Nile tilapia ,030104 developmental biology ,chemistry ,human activities ,DNA - Abstract
Multiwalled carbon nanotubes (MWCNTs), nanographene oxide (NGO), and gold nanorods (NRs) can be functionalized and complexed to DNA to promote efficient gene delivery to Nile tilapia spermatogonial stem cells inducing less cell death than electroporation and the commercial reagents tested. Therefore, nanomaterials can contribute to achieve fish transgenesis.
- Published
- 2016
- Full Text
- View/download PDF
48. Graphene-based nanomaterials: biological and medical applications and toxicity
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Rodrigo R. Resende, Marina Ladeira, Vânia Aparecida Mendes Goulart, Fernanda Maria Policarpo Tonelli, Anderson K. Santos, Luiz Orlando Ladeira, Katia N. Gomes, and Eudes Lorençon
- Subjects
Materials science ,Tissue Engineering ,Biocompatibility ,Graphene ,Biomedical Engineering ,Medicine (miscellaneous) ,Bioengineering ,Nanotechnology ,Carbon nanotube ,Development ,Gene delivery ,Nanostructures ,law.invention ,Nanomaterials ,Microscopy, Electron, Transmission ,Tissue engineering ,law ,Drug delivery ,Graphite ,General Materials Science ,Biosensor - Abstract
Graphene and its derivatives, due to a wide range of unique properties that they possess, can be used as starting material for the synthesis of useful nanocomplexes for innovative therapeutic strategies and biodiagnostics. Here, we summarize the latest progress in graphene and its derivatives and their potential applications for drug delivery, gene delivery, biosensor and tissue engineering. A simple comparison with carbon nanotubes uses in biomedicine is also presented. We also discuss their in vitro and in vivo toxicity and biocompatibility in three different life kingdoms (bacterial, mammalian and plant cells). All aspects of how graphene is internalized after in vivo administration or in vitro cell exposure were brought about, and explain how blood–brain barrier can be overlapped by graphene nanomaterials.
- Published
- 2015
- Full Text
- View/download PDF
49. Functionalized nanomaterials: are they effective to perform gene delivery to difficult-to-transfect cells with no cytotoxicity?
- Author
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Mauro Cunha Xavier Pinto, S. R. A. Scalzo Junior, F. H. P. de Macedo, Jader S. Cruz, Luiz Orlando Ladeira, F. G. Pacheco, Fernanda Maria Policarpo Tonelli, Nicole de Cássia Oliveira Paiva, Luiz R. França, Silvia Guatimosim, Samyra M.S.N. Lacerda, J. D. Corrêa Junior, and R. R. Resende
- Subjects
animal structures ,Chemistry ,viruses ,fungi ,Cationic polymerization ,Nanotechnology ,Transfection ,Gene delivery ,Multiwalled carbon ,Cell Line ,Nanostructures ,Nanomaterials ,Mice ,embryonic structures ,Animals ,General Materials Science ,Nanorod ,Impalefection ,Cytotoxicity - Abstract
Nanodiamonds (NDs), multiwalled carbon nanotubes (MWCNTs) and gold nanorods (NRs) can be functionalized to promote gene delivery to hard-to-transfect cells with higher transfection efficiency than cationic lipids, and inducing less cell death.
- Published
- 2015
- Full Text
- View/download PDF
50. Gene delivery to Nile tilapia cells for transgenesis and the role of PI3K-c2α in angiogenesis
- Author
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Rodrigo R. Resende, M. S. Procópio, Luiz R. França, Breno Luiz Sales Lemos, Fernanda Maria Policarpo Tonelli, and Samyra M.S.N. Lacerda
- Subjects
Male ,0301 basic medicine ,Embryo, Nonmammalian ,Animal, Genetically Modified ,Zygote ,Phosphatidylinositol 3 Kinase ,Procedures ,Animals, Genetically Modified ,Phosphatidylinositol 3-Kinases ,Nile tilapia ,0302 clinical medicine ,Transduction, Genetic ,Multidisciplinary ,Adult Germline Stem Cells ,biology ,Gene Expression Regulation, Developmental ,Cichlids ,Micro-injection ,Cell biology ,Transgenesis ,medicine.anatomical_structure ,Larva ,Cichlid ,Deficiency ,Gamete ,Female ,Transgenic Animals ,Phosphatidylinositol 3-kinases ,Microinjections ,Transgene ,Neovascularization, Physiologic ,Gene delivery ,Germ Cell ,Article ,03 medical and health sciences ,Genetics ,medicine ,Animals ,Microinjection ,Transplantation ,Animal ,Vascularization ,biology.organism_classification ,Sperm ,Molecular biology ,Metabolism ,Germ Cells ,030104 developmental biology ,Gene Expression Regulation ,Growth, Development And Aging ,Adult Stem Cell ,Mutation ,Angiogenesis ,Cytology ,030217 neurology & neurosurgery - Abstract
Microinjection is commonly performed to achieve fish transgenesis; however, due to difficulties associated with this technique, new strategies are being developed. Here we evaluate the potential of lentiviral particles to genetically modify Nile tilapia cells to achieve transgenesis using three different approaches: spermatogonial stem cell (SSC) genetic modification and transplantation (SC), in vivo transduction of gametes (GT), and fertilised egg transduction (ET). The SC protocol using larvae generates animals with sustained production of modified sperm (80% of animals with 77% maximum sperm fluorescence [MSF]), but is a time-consuming protocol (sexual maturity in Nile tilapia is achieved at 6 months of age). GT is a faster technique, but the modified gamete production is temporary (70% of animals with 52% MSF). ET is an easier way to obtain mosaic transgenic animals compared to microinjection of eggs, but non-site-directed integration in the fish genome can be a problem. In this study, PI3Kc2α gene disruption impaired development during the embryo stage and caused premature death. The manipulator should choose a technique based on the time available for transgenic obtainment and if this generation is required to be continuous or not.
- Published
- 2017
- Full Text
- View/download PDF
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