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Cochlospermum regium roots are used in popular medicine and its extract has diverse phytochemical molecules some with antimicrobial activity, consequently exposing this specie to genetic erosion risks. Thus, the objective of this study was to develop an in vitro multiplication protocol using chemical sterilization of culture medium. Therefore, explants obtained from apical buds of C. regium seedlings were inoculated into with 0.05mg L-1 NAA and 1mg L-1 BAP sterilized by chemical agent sodium hypochlorite (NaOCl) at 0.001%, 0.003% and 0.005% of active chlorine (Cl). Autoclaved culture medium was used as control. Result showed that the contamination by bacterial at 91 days of cultivation was significantly (P

We evaluated the supplementation of the MS culture medium with active chlorine and activated charcoal for the in vitro culture of Bambusa vulgaris. Lateral shoots were collected from field-cultivated plants to obtain explants, which consisted of nodal segments of 1-2 cm length, containing one axillary bud. The MS culture medium was supplemented with the following treatments: E1= 0.004 % v v-1 of active chlorine + 4 g L-1 of charcoal; E2= 0.004 % v v-1 of active chlorine; E3= 4 g L-1 charcoal and E4= control (no active chlorine and no charcoal). The percentage of oxidation, fungal and bacterial contamination, explant establishment, survival, sprout and adventitious rooting were evaluated every 21 d, during 63 d. The use of active chlorine reduced the fungal (28 %) and bacterial (38 %) contamination, being the treatment with the highest percentage of established explants (16 %), survival (68 %) and sprout induction (95 %) after 63 d. After 36 days from the beginning of the multiplication stage, some explants presented spontaneous rooting, without the application of plant growth regulators. An anatomical analysis of sprouts and roots revealed the presence of meristematic zones. Furthermore, it indicated that the emergence of adventitious roots occurred from the nodal region in association with axillary buds. Our results contribute toward the development of more efficient protocols for the in vitro propagation of B. vulgaris, from which we recommend using active chlorine for further assessments., En el estudio se evaluó el suplemento de cloro activo y carbono activado en el cultivo in vitro de Bambusa vulgaris. Brotes laterales fueron colectados de las plantas cultivadas en el campo para obtener explantes. Los explantes consistieron en segmentos nodales de 1-2 cm de longitud que contenían una yema axilar. El medio MS se suplementó con los siguientes tratamientos: E1 = 0,004 % v v-1 de cloro activo + 4 g L-1 de carbón; E2 = 0,004 % v v-1 de cloro activo; E3 = 4 g L-1 de carbón y E4 = control (sin suplemento con cloro activo y sin carbón). El porcentaje de oxidación, la contaminación por hongos y bacterias, el establecimiento del explante, la supervivencia, la inducción del brote y enraizamiento se evaluaron durante 63 días. El uso de cloro activo redujo la contaminación por hongos (28 %) y bacterianas (38 %), siendo el tratamiento con mayor porcentaje de explantes establecidos (16 %), supervivencia (68 %) y inducción de brotes (95 %). Después de 36 días, algunos explantes presentaron enraizamiento espontáneo, sin aplicación de reguladores de crecimiento. Un análisis anatómico de los brotes y raíces mostró zonas meristemáticas, la conexión entre el sistema vascular del tallo y las hojas y la aparición de raíces adventicias en la región nodal en asociación con los brotes axilares. Los resultados contribuyen al desarrollo de protocolos más eficientes para la propagación in vitro de B. vulgaris, de los cuales se recomienda el uso de cloro activo para futuras evaluaciones.