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1. Emergence of Delta and Omicron variants carrying resistance-associated mutations in immunocompromised patients undergoing sotrovimab treatment with long-term viral excretion

Author

Andrés, Cristina, González-Sánchez, Alejandra, Jiménez, Moraima, Márquez-Algaba, Ester, Piñana, Maria, Fernández-Naval, Candela, Esperalba, Juliana, Saubi, Narcís, Quer, Josep, Rando-Segura, Ariadna, Miarons, Marta, Codina, Maria Gema, Ruiz-Camps, Isabel, Pumarola, Tomàs, Abrisqueta, Pau, and Antón, Andrés

Published
2023
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3. Cellular and humoral immunogenicity of the mRNA-1273 SARS-CoV-2 vaccine in patients with hematologic malignancies

Author

Jiménez, Moraima, Roldán, Elisa, Fernández-Naval, Candela, Villacampa, Guillermo, Martinez-Gallo, Mónica, Medina-Gil, Daniel, Peralta-Garzón, Soraya, Pujadas, Gemma, Hernández, Cristina, Pagès, Carlota, Gironella, Mercedes, Fox, Laura, Orti, Guillermo, Barba, Pere, Pumarola, Tomás, Cabirta, Alba, Catalá, Eva, Valentín, Mercedes, Marín-Niebla, Ana, Orfao, Alberto, González, Marcos, Campins, Magda, Ruiz-Camps, Isabel, Valcárcel, David, Bosch, Francesc, Hernández, Manuel, Crespo, Marta, Esperalba, Juliana, and Abrisqueta, Pau

Published
2022
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4. Global phylogeny of Treponema pallidum lineages reveals recent expansion and spread of contemporary syphilis

Author

Beale, Mathew A., Marks, Michael, Cole, Michelle J., Lee, Min-Kuang, Pitt, Rachel, Ruis, Christopher, Balla, Eszter, Crucitti, Tania, Ewens, Michael, Fernández-Naval, Candela, Grankvist, Anna, Guiver, Malcolm, Kenyon, Chris R., Khairullin, Rafil, Kularatne, Ranmini, Arando, Maider, Molini, Barbara J., Obukhov, Andrey, Page, Emma E., Petrovay, Fruzsina, Rietmeijer, Cornelis, Rowley, Dominic, Shokoples, Sandy, Smit, Erasmus, Sweeney, Emma L., Taiaroa, George, Vera, Jaime H., Wennerås, Christine, Whiley, David M., Williamson, Deborah A., Hughes, Gwenda, Naidu, Prenilla, Unemo, Magnus, Krajden, Mel, Lukehart, Sheila A., Morshed, Muhammad G., Fifer, Helen, and Thomson, Nicholas R.

Published
2021
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5. Role of SARS-CoV-2-specific memory B cells promoting immune protection after booster vaccination in solid organ transplantation.

Author

Donadeu, Laura, Gomez-Olles, Susana, Casanova, Franc, Torija, Alba, Lopez-Meseguer, Manuel, Boada-Pérez, Meritxell, Kervella, Delphine, Crespo, Elena, Carrera-Muñoz, Claudia, Campos-Varela, Isabel, Castells, Lluís, Cortese, Maria F., Esperalba, Juliana, Fernández-Naval, Candela, Quintero, Jesús, Muñoz, Marina, Agüero, Fernando, Gonzalez-Costello, José, Lladó, Laura, and Favà, Alexandre

Subjects
SARS-CoV-2, IMMUNOLOGIC memory, BOOSTER vaccines, COVID-19, VACCINE effectiveness
Abstract

Introduction: Solid organ transplant (SOT) recipients display weak seroconversion and neutralizing antibody (NAb) responses after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccination and remain at risk of severe coronavirus disease 2019 (COVID-19). While B-cell memory is the hallmark of serological immunity, its role in driving successful vaccine responses and providing immune protection in SOT patients remains unclear. Methods: We investigated the function and interplay of SARS-CoV-2-specific memory B cells (mBc), different cytokineproducing T cells, and cross-reactive NAb in driving seroconversion and protection against COVID-19 in two cohorts. First, we studied a large cohort of 148 SOT recipients and 32 immunocompetent individuals who underwent several vaccinations. Subsequently, we assessed 25 SOT patients participating in a randomized controlled trial to compare two different immunosuppressive strategies for allowing successful seroconversion and memory-cell responses after booster vaccination. Results: We corroborate previous findings that B- and T-cell memory responses are weaker and more delayed in SOT patients than in immunocompetent (IC) individuals; however, within the SOT cohort, we found that these responses are relatively stronger and more robust in patients not receiving mycophenolate mofetil (MMF)-based therapies. Anti-spike IgG titers strongly correlated with RBD-specific IgG-producing mBc, with both displaying broad viral cross reactivity. Prebooster SARS-CoV-2-specific mBc and IL-2-producing T cells accurately predicted Nab seroconversion (AUC, 0.828) and protection against severe COVID-19. While switching unresponsive SOT patients from calcineurin inhibitors (CNI)/MMF to a low-exposure CNI/mTOR-i regimen favored wider SARS-CoV-2-specific immune responses after a fourth booster vaccination, preformed RBD-specific mBc predicted NAb seroconversion. Discussion: Our study adds new insights into the pathobiology of immune memory and highlights the pivotal role of SARS-CoV-2-specific mBc in promoting immune protection inSOT patients. [ABSTRACT FROM AUTHOR]

Published
2024
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7. Kinetics of cellular and humoral immunogenicity and effectiveness of SARS‐CoV ‐2 booster vaccination in hematologic neoplasms

Author

Jiménez, Moraima, primary, Fernández‐Naval, Candela, additional, Navarro, Víctor, additional, Novoa, Sandra, additional, Martinez‐Gallo, Mónica, additional, Medina, Daniel, additional, Andrés, Cristina, additional, Antón, Andrés, additional, Peralta, Soraya, additional, Pujadas, Gemma, additional, Hernández, Cristina, additional, Pagès, Carlota, additional, Roldán, Elisa, additional, Pumarola, Tomás, additional, Gironella, Mercedes, additional, Ruiz‐Camps, Isabel, additional, Martínez‐Gómez, Xavier, additional, Valcárcel, David, additional, Hernández, Manuel, additional, Bosch, Francesc, additional, Crespo, Marta, additional, Esperalba, Juliana, additional, and Abrisqueta, Pau, additional

Published
2023
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9. Clinical Impact of Sars-Cov-2 Vaccination in COVID-19 Outcomes in Patients Diagnosed with Hematologic Malignancies: Real-World Evidence of Two Years of Pandemic

Author

Jiménez, Moraima, primary, Novoa Jáuregui, Sandra, additional, Fernández-Naval, Candela, additional, Bosch Schips, Marc, additional, Muzio, Sofía, additional, Navarro Garcés, Víctor, additional, Andrés, Cristina, additional, Martínez-Gallo, Mónica, additional, Antón, Andrés, additional, Cabirta, Alba, additional, Serna, Angel, additional, Medina, Daniel, additional, Peralta, Soraya, additional, Pujadas, Gemma, additional, Hernandez, Cristina, additional, Pagès, Carlota, additional, Pumarola, Tomás, additional, Valentín, Mercedes, additional, Valcárcel, David, additional, Hernández, Manuel, additional, Ruiz-Camps, Isabel, additional, Crespo, Marta, additional, Esperalba, Juliana, additional, Abrisqueta, Pau, additional, and Bosch, Francesc, additional

Published
2022
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10. Is humoral and cellular response to SARS-CoV-2 vaccine modified by DMT in patients with multiple sclerosis and other autoimmune diseases?

Author

Zabalza, Ana, primary, Arrambide, Georgina, additional, Otero-Romero, Susana, additional, Pappolla, Agustín, additional, Tagliani, Paula, additional, López-Maza, Samuel, additional, Cárdenas-Robledo, Simón, additional, Esperalba, Juliana, additional, Fernández-Naval, Candela, additional, Martínez-Gallo, Monica, additional, Castillo, Mireia, additional, Bonastre, Mercè, additional, Resina-Salles, Mireia, additional, Bertran, Jordina, additional, Rodriguez-Barranco, Marta, additional, Carbonell-Mirabent, Pere, additional, Gonzalez, Marina, additional, Merchan, Miguel, additional, Quiroga-Varela, Ana, additional, Miguela, Albert, additional, Gómez, Imma, additional, Álvarez, Gary, additional, Robles, René, additional, Perez del Campo, Dúnia, additional, Queralt, Xavier, additional, Soler, Maria José, additional, Agraz, Irene, additional, Martinez-Valle, Fernando, additional, Rodríguez-Acevedo, Breogán, additional, Midaglia, Luciana, additional, Vidal-Jordana, Ángela, additional, Cobo-Calvo, Álvaro, additional, Tur, Carmen, additional, Galan, Ingrid, additional, Castillo, Joaquín, additional, Río, Jordi, additional, Espejo, Carmen, additional, Comabella, Manuel, additional, Nos, Carlos, additional, Sastre-Garriga, Jaume, additional, Ramió-Torrentà, Lluís, additional, Tintoré, Mar, additional, and Montalban, Xavier, additional

Published
2022
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11. Label-Free Plasmonic Biosensor for Rapid, Quantitative, and Highly Sensitive COVID-19 Serology: Implementation and Clinical Validation

Author

Calvo-Lozano, Olalla, primary, Sierra, Miquel, additional, Soler, Maria, additional, Estévez, Maria Carmen, additional, Chiscano-Camón, Luis, additional, Ruiz-Sanmartin, Adolfo, additional, Ruiz-Rodriguez, Juan Carlos, additional, Ferrer, Ricard, additional, González-López, Juan José, additional, Esperalba, Juliana, additional, Fernández-Naval, Candela, additional, Bueno, Leticia, additional, López-Aladid, Ruben, additional, Torres, Antoni, additional, Fernández-Barat, Laia, additional, Attoumani, Sarah, additional, Charrel, Rémi, additional, Coutard, Bruno, additional, and Lechuga, Laura M., additional

Published
2021
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12. Analytical Evaluation of Dried Blood Spot and Rapid Diagnostic Test as a New Strategy for Serological Community Screening for Chronic Chagas Disease

Author

Silgado, Aroa, primary, Gual-Gonzalez, Lídia, additional, Sánchez-Montalvá, Adrián, additional, Oliveira-Souto, Inés, additional, Goterris, Lidia, additional, Serre-Delcor, Nuria, additional, Esperalba, Juliana, additional, Gomez-i-Prat, Jordi, additional, Fernández-Naval, Candela, additional, Molina, Israel, additional, Pumarola, Tomas, additional, and Sulleiro, Elena, additional

Published
2021
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13. Multilocus sequence typing ofTreponema pallidumsubsp.pallidumin Barcelona

Author

Fernández-Naval, Candela, primary, Arando, Maider, additional, Espasa, Mateu, additional, Antón, Andrés, additional, Fernández-Huerta, Miguel, additional, Silgado, Aroa, additional, Pinatar, Cristina, additional, Zarzuela, Francesc, additional, González-López, Juan José, additional, Serra-Pladevall, Judit, additional, Sulleiro, Elena, additional, Pumarola, Tomàs, additional, Vall-Mayans, Martí, additional, and Esperalba, Juliana, additional

Published
2021
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14. Risk of SARS-CoV-2 Infection in Previously Infected and Non-Infected Cohorts of Health Workers at High Risk of Exposure

Author

Sánchez-Montalvá, Adrián, primary, Fernández-Naval, Candela, additional, Antón, Andrés, additional, Durà, Xavier, additional, Vimes, Alba, additional, Silgado, Aroa, additional, Velásquez-Orozco, Fernando, additional, Espinosa-Pereiro, Juan, additional, Salvador, Fernando, additional, Pumarola, Tomás, additional, Almirante, Benito, additional, and Esperalba, Juliana, additional

Published
2021
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15. Commercialized kit to assess T-cell responses against SARS-CoV-2 S peptides. A pilot study in Health Care Workers

Author

Martínez-Gallo, Mónica, primary, Esperalba-Esquerra, Juliana, additional, Pujol-Borrell, Ricardo, additional, Sandá, Víctor, additional, Arrese-Muñoz, Iria, additional, Fernández-Naval, Candela, additional, Antón-Pagarolas, Andrés, additional, Cardona, Victoria, additional, Labrador-Horrillo, Moisés, additional, Pumarola-Suñé, Tomás, additional, and Hernandéz-González, Manuel, additional

Published
2021
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16. Contemporary syphilis is characterised by rapid global spread of pandemic Treponema pallidum lineages

Author

Beale, Mathew A., primary, Marks, Michael, additional, Cole, Michelle J., additional, Lee, Min-Kuang, additional, Pitt, Rachel, additional, Ruis, Christopher, additional, Balla, Eszter, additional, Crucitti, Tania, additional, Ewens, Michael, additional, Fernández-Naval, Candela, additional, Grankvist, Anna, additional, Guiver, Malcolm, additional, Kenyon, Chris R., additional, Khairulin, Rafil, additional, Kularatne, Ranmini, additional, Arando, Maider, additional, Molini, Barbara J., additional, Obukhov, Andrey, additional, Page, Emma E., additional, Petrovay, Fruzsina, additional, Rietmeijer, Cornelis, additional, Rowley, Dominic, additional, Shokoples, Sandy, additional, Smit, Erasmus, additional, Sweeney, Emma L., additional, Taiaroa, George, additional, Vera, Jaime H., additional, Wennerås, Christine, additional, Whiley, David M., additional, Williamson, Deborah A., additional, Hughes, Gwenda, additional, Naidu, Prenilla, additional, Unemo, Magnus, additional, Krajden, Mel, additional, Lukehart, Sheila A., additional, Morshed, Muhammad G., additional, Fifer, Helen, additional, and Thomson, Nicholas R., additional

Published
2021
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17. Single-Locus-Sequence-Based Typing of the mgpB Gene Reveals Transmission Dynamics in Mycoplasma genitalium

Author

Fernández-Huerta, Miguel, primary, Serra-Pladevall, Judit, additional, Esperalba, Juliana, additional, Moreno-Mingorance, Albert, additional, Fernández-Naval, Candela, additional, Barberá, María-Jesús, additional, Aparicio, David, additional, Pich, Oscar Q., additional, Pumarola, Tomàs, additional, Jensen, Jorgen S., additional, and Espasa, Mateu, additional

Published
2020
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18. Label-Free Plasmonic Biosensor for Rapid, Quantitative, and Highly Sensitive COVID-19 Serology: Implementation and Clinical Validation.

Author

Calvo-Lozano, Olalla, Sierra, Miquel, Soler, Maria, Estévez, Maria Carmen, Chiscano-Camón, Luis, Ruiz-Sanmartin, Adolfo, Ruiz-Rodriguez, Juan Carlos, Ferrer, Ricard, González-López, Juan José, Esperalba, Juliana, Fernández-Naval, Candela, Bueno, Leticia, López-Aladid, Ruben, Torres, Antoni, Fernández-Barat, Laia, Attoumani, Sarah, Charrel, Rémi, Coutard, Bruno, and Lechuga, Laura M.

Published
2022
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19. 474 - Vaccination Against Sars-Cov-2 Induces Robust Humoral Immune Response in Patients with Previous Hematologic Stem Cell Transplantation

Author

Roldan, Elisa, Del Carmen Jimenez, Moraima, Villacampa, Guillermo, Fernandez-Naval, Candela, Perez, Ana, Fox, Laura, Orti, Guillermo, Salamero, Olga, Barba, Pere, Campins, Magda, Esperalba, Juliana, Ruiz-Camps, Isabel, Crespo, Marta, Hernández-González, Manuel, Bosch, Francesc, Abrisqueta, Pau, and Valcarcel, David

Published
2022
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20. Multicenter Clinical Evaluation of a Novel Multiplex Real-Time PCR (qPCR) Assay for Detection of Fluoroquinolone Resistance in Mycoplasma genitalium

Author

Fernández-Huerta, Miguel, primary, Bodiyabadu, Kaveesha, additional, Esperalba, Juliana, additional, Bradshaw, Catriona S., additional, Serra-Pladevall, Judit, additional, Garland, Suzanne M., additional, Fernández-Naval, Candela, additional, Jensen, Jorgen S., additional, Pumarola, Tomàs, additional, Ebeyan, Samantha, additional, Lundgren, Marie, additional, Tan, Lit Yeen, additional, Espasa, Mateu, additional, and Murray, Gerald L., additional

Published
2019
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21. Enhanced Molecular Typing and Macrolide and Tetracycline-Resistance Mutations of Treponema Pallidum in Barcelona

Author

Fernández-Naval, Candela, primary, Arando, Maider, additional, Espasa, Mateu, additional, Antón, Andrés, additional, Fernández-Huerta, Miguel, additional, Silgado, Aroa, additional, Jimenez, Inmaculada, additional, Villatoro, Ana M, additional, González-López, Juan J, additional, Serra-Pladevall, Judit, additional, Sulleiro, Elena, additional, Pumarola, Tomàs, additional, Vall-Mayans, Martí, additional, and Esperalba, Juliana, additional

Published
2019
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23. Enhanced Molecular Typing and Macrolide and Tetracycline-Resistance Mutations of Treponema Pallidumin Barcelona

Author

Fernández-Naval, Candela, Arando, Maider, Espasa, Mateu, Antón, Andrés, Fernández-Huerta, Miguel, Silgado, Aroa, Jimenez, Inmaculada, Villatoro, Ana M, González-López, Juan J, Serra-Pladevall, Judit, Sulleiro, Elena, Pumarola, Tomàs, Vall-Mayans, Martí, and Esperalba, Juliana

Abstract

Aim:To describe the molecular types of Treponema pallidumand the proportion of macrolide and tetracycline resistance mutations in Barcelona. Materials & methods:Molecular type was determined using the Enhanced-CDC Typing system and antibiotic resistance was determined by sequencing the 23S and 16S rRNA genes. Results:A total of 183 patients were enrolled and 213 specimens (99 ulcers, 114 bloods) were collected. Sixty-two (70.5%) of 88 ulcers and 0 (0%) of bloods T. pallidum-DNA containing samples were fully typed. Up to 21 different strain types were identified (14d/g in 27.4%; 14f/g in 14.5%). Macrolide resistance mutations were present in 95% and tetracycline in 0%. Conclusion:Several different strains co-exist in Barcelona with a high proportion of macrolide resistance and absence of tetracycline resistance.

Published
2019
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24. Mycoplasma genitaliummacrolide resistance update: Rate among a 2016–2017 cohort of patients in Barcelona, Spain

Author

Fernández-Huerta, Miguel, Vall, Martí, Fernández-Naval, Candela, Barberá, María-Jesús, Arando, Maider, López, Luis, Andreu, Antonia, Pumarola, Tomàs, Serra-Pladevall, Judit, Esperalba, Juliana, and Espasa, Mateu

Abstract

Mycoplasma genitaliumis a major cause of urethritis and other genital syndromes. Antibiotic resistance, especially to macrolides, is increasing at an alarming rate worldwide. The aim of this study was to estimate the rate of macrolide resistance in M. genitaliumamong a 2016–2017 cohort of patients in Barcelona, Spain; and to compare this estimate with previous data from 2013 to 2014 in this region.

Published
2024
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25. Commercialized kits to assess T-cell responses against SARS-CoV-2 S peptides. A pilot study in health care workers

Author

Martínez-Gallo, Mónica, Esperalba, Juliana, Pujol-Borrell, Ricardo, Sandá, Víctor, Arrese-Muñoz, Iria, Fernández-Naval, Candela, Antón, Andrés, Cardona, Victoria, Labrador-Horrillo, Moisés, Pumarola, Tomás, and Hernandéz-González, Manuel

Abstract

It is crucial to assess the levels of protection generated by natural infection or SARS-CoV-2 vaccines, mainly in individuals professionally exposed and in vulnerable groups. Measuring T-cell responses may complement antibody tests currently in use as correlates of protection. Our aim was to assess the feasibility of a validated assay of T-cell responses.

Published
2021
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26. Multilocus sequence typing of Treponema pallidumsubsp. pallidumin Barcelona

Author

Fernández-Naval, Candela, Arando, Maider, Espasa, Mateu, Antón, Andrés, Fernández-Huerta, Miguel, Silgado, Aroa, Pinatar, Cristina, Zarzuela, Francesc, González-López, Juan José, Serra-Pladevall, Judit, Sulleiro, Elena, Pumarola, Tomàs, Vall-Mayans, Martí, and Esperalba, Juliana

Abstract

Aim:To implement the multilocus sequence typing (MLST) methodology in syphilis samples previously characterized by enhanced CDC typing (ECDCT) and macrolide resistance. Materials & methods:MLST was performed on genital ulcer and blood samples by analyzing a region of the tp0136, tp0548and tp0705lociusing Sanger sequencing. Results:Up to 59/85 (69.4%) of genital ulcer and 4/39 (10.3%) of whole blood samples were fully typed. The most frequent profiles were 1.3.1 (56%) and 1.1.1 (11%). All the 1.3.1 samples typed carried the A2058G mutation, responsible for macrolide resistance. MLST and ECDCT showed similar overall typing yields. Conclusion:Several allelic profiles of T. pallidumsubsp. pallidumwere identified and classified into two major genetic clades in Barcelona. Our results were similar to that described in Europe.

Published
2021
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27. Single-Locus-Sequence-Based Typing of the mgpBGene Reveals Transmission Dynamics in Mycoplasma genitalium

Author

Fernández-Huerta, Miguel, Serra-Pladevall, Judit, Esperalba, Juliana, Moreno-Mingorance, Albert, Fernández-Naval, Candela, Barberá, María-Jesús, Aparicio, David, Pich, Oscar Q., Pumarola, Tomàs, Jensen, Jorgen S., and Espasa, Mateu

Abstract

Sexually transmitted infections (STIs) by Mycoplasma genitaliumare a major problem worldwide, especially given their marked and rapid propensity for developing antimicrobial resistance. Since very few treatment options exist, clinicians face an important challenge in the management of the infection. In this scenario, little is known regarding the transmission dynamics of M. genitaliumand the epidemiology of antimicrobial resistance. This mgpB-based molecular typing study, conducted among 54 asymptomatically infected individuals prospectively recruited from an STI screening service, reveals two distinct epidemiological clusters that significantly correlate with sexual conduct in heterosexuals and men who have sex with men (MSM), respectively.

Published
2020
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28. Global phylogeny of Treponema pallidum lineages reveals recent expansion and spread of contemporary syphilis

Author

Beale, Mathew A, Marks, Michael, Cole, Michelle J, Lee, Min-Kuang, Pitt, Rachel, Ruis, Christopher, Balla, Eszter, Crucitti, Tania, Ewens, Michael, Fernández-Naval, Candela, Grankvist, Anna, Guiver, Malcolm, Kenyon, Chris R, Khairullin, Rafil, Kularatne, Ranmini, Arando, Maider, Molini, Barbara J, Obukhov, Andrey, Page, Emma E, Petrovay, Fruzsina, Rietmeijer, Cornelis, Rowley, Dominic, Shokoples, Sandy, Smit, Erasmus, Sweeney, Emma L, Taiaroa, George, Vera, Jaime H, Wennerås, Christine, Whiley, David M, Williamson, Deborah A, Hughes, Gwenda, Naidu, Prenilla, Unemo, Magnus, Krajden, Mel, Lukehart, Sheila A, Morshed, Muhammad G, Fifer, Helen, and Thomson, Nicholas R

Subjects
Drug Resistance, Bacterial, Humans, Macrolides, Syphilis, Treponema pallidum, 15. Life on land, Genome, Bacterial, Phylogeny, 3. Good health, Anti-Bacterial Agents
Abstract

Funder: Queensland Government, Syphilis, which is caused by the sexually transmitted bacterium Treponema pallidum subsp. pallidum, has an estimated 6.3 million cases worldwide per annum. In the past ten years, the incidence of syphilis has increased by more than 150% in some high-income countries, but the evolution and epidemiology of the epidemic are poorly understood. To characterize the global population structure of T. pallidum, we assembled a geographically and temporally diverse collection of 726 genomes from 626 clinical and 100 laboratory samples collected in 23 countries. We applied phylogenetic analyses and clustering, and found that the global syphilis population comprises just two deeply branching lineages, Nichols and SS14. Both lineages are currently circulating in 12 of the 23 countries sampled. We subdivided T. p. pallidum into 17 distinct sublineages to provide further phylodynamic resolution. Importantly, two Nichols sublineages have expanded clonally across 9 countries contemporaneously with SS14. Moreover, pairwise genome analyses revealed examples of isolates collected within the last 20 years from 14 different countries that had genetically identical core genomes, which might indicate frequent exchange through international transmission. It is striking that most samples collected before 1983 are phylogenetically distinct from more recently isolated sublineages. Using Bayesian temporal analysis, we detected a population bottleneck occurring during the late 1990s, followed by rapid population expansion in the 2000s that was driven by the dominant T. pallidum sublineages circulating today. This expansion may be linked to changing epidemiology, immune evasion or fitness under antimicrobial selection pressure, since many of the contemporary syphilis lineages we have characterized are resistant to macrolides.

29. Is humoral and cellular response to SARS-CoV-2 vaccine modified by DMT in patients with multiple sclerosis and other autoimmune diseases?

Author

Zabalza A, Arrambide G, Otero-Romero S, Pappolla A, Tagliani P, López-Maza S, Cárdenas-Robledo S, Esperalba J, Fernández-Naval C, Martínez-Gallo M, Castillo M, Bonastre M, Resina-Salles M, Bertran J, Rodriguez-Barranco M, Carbonell-Mirabent P, Gonzalez M, Merchan M, Quiroga-Varela A, Miguela A, Gómez I, Álvarez G, Robles R, Perez Del Campo D, Queralt X, Soler MJ, Agraz I, Martinez-Valle F, Rodríguez-Acevedo B, Midaglia L, Vidal-Jordana Á, Cobo-Calvo Á, Tur C, Galan I, Castillo J, Río J, Espejo C, Comabella M, Nos C, Sastre-Garriga J, Ramió-Torrentà L, Tintoré M, and Montalban X

Subjects
Antibodies, Viral, COVID-19 Vaccines, Humans, SARS-CoV-2, Vaccination, COVID-19 prevention & control, Multiple Sclerosis drug therapy
Abstract

Background: The effect of disease-modifying therapies on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine response is unclear., Objectives: We aim to determine the immunological responses to SARS-CoV-2 in multiple sclerosis (MS) and anti-CD20-treated patients with other autoimmune diseases (AID)., Methods: Humoral and cellular responses we determined before and 30-90 days after vaccination in patients with MS and anti-CD20-treated patients with other AID in two Catalan centers., Results: 457 patients were enrolled. Findings showed that humoral response decreased under anti-CD20s or sphingosine 1-phosphate receptor modulators (S1PRM) and with longer treatment duration and increased after 4.5 months from the last anti-CD20 infusion. Cellular response decreased in S1PRM-treated. Patients on anti-CD20 can present cellular responses even in the absence of antibodies., Conclusion: Anti-CD20s and S1PRM modify the immunological responses to SARS-CoV-2 vaccines.

Published
2022
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30. Multilocus sequence typing of Treponema pallidum subsp. pallidum in Barcelona.

Author

Fernández-Naval C, Arando M, Espasa M, Antón A, Fernández-Huerta M, Silgado A, Pinatar C, Zarzuela F, González-López JJ, Serra-Pladevall J, Sulleiro E, Pumarola T, Vall-Mayans M, and Esperalba J

Subjects
Humans, Macrolides pharmacology, Multilocus Sequence Typing, Spain, Ulcer, Anti-Bacterial Agents pharmacology, Drug Resistance, Bacterial genetics, Syphilis microbiology, Treponema classification
Abstract

Aim: To implement the multilocus sequence typing (MLST) methodology in syphilis samples previously characterized by enhanced CDC typing (ECDCT) and macrolide resistance. Materials & methods: MLST was performed on genital ulcer and blood samples by analyzing a region of the tp0136, tp0548 and tp0705 loci using Sanger sequencing. Results: Up to 59/85 (69.4%) of genital ulcer and 4/39 (10.3%) of whole blood samples were fully typed. The most frequent profiles were 1.3.1 (56%) and 1.1.1 (11%). All the 1.3.1 samples typed carried the A2058G mutation, responsible for macrolide resistance. MLST and ECDCT showed similar overall typing yields. Conclusion: Several allelic profiles of T. pallidum subsp. pallidum were identified and classified into two major genetic clades in Barcelona. Our results were similar to that described in Europe.

Published
2021
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