1. Microstructured molecular BIO-gratings by means of UV induced denaturation - INVITED
- Author
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Juste-Dolz Augusto, Delgado-Pinar Martina, Avella-Oliver Miquel, Fernández Estrella, Cruz Jose Luis, Andrés Miguel V., and Maquieira Ángel
- Subjects
Physics ,QC1-999 - Abstract
Rapid, reliable and low cost techniques to fabricate biosensors is a hot topic nowadays. Here, we present a BIO-grating fabricated by means of local, selective denaturing of molecules using UV radiation. A phase-mask is used to generate an interferometric pattern of 1420 nm pitch that, when illuminating a biolayer of BSA molecules lead to its periodic deactivation. After the biorecognition of the specific antibody, aBSA, a BIO-grating is generated due to the height difference between the protein, and the complex protein + antibody. We present the optimization of the fabrication of the BIO-gratings and their AFM characterization. Also, the biosensor performance in terms of limit of detection and limit of quantification will be presented.
- Published
- 2023
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