38 results on '"Fernández DI"'
Search Results
2. Literatura en la formación del profesor de lengua extranjera
- Author
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Fernández Di Turi, María Teresa and Juana Sagaray
- Published
- 2015
- Full Text
- View/download PDF
3. Mycorrhizal fungi isolated from native terrestrial orchids of pristine regions in Córdoba (Argentina)
- Author
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Agustina Fernández Di Pardo, Viviana M. Chiocchio, Viviana Barrera, Roxana P. Colombo, Alicia E. Martinez, Laura Gasoni, and Alicia M. Godeas
- Subjects
Rhizoctonia ,Epulorhiza ,orquídeas terrestres ,micorrizas ,ITS-rDNA ,Biology (General) ,QH301-705.5 - Abstract
Orchidaceae is a highly dependent group on the Rhizoctonia complex that includes Ceratorhiza, Moniliopsis, Epulorhiza and Rhizoctonia, for seed germination and the development of new orchid plants. Thus, the isolation and identification of orchid mycorrhizal fungi are important to understand the orchid-fungus relationship, which can lead to the development of efficient conservation strategies by in vivo germination of seeds from endangered orchid plants. The aim of our work was to isolate and characterize the different mycorrhizal fungi found in roots of terrestrial orchids from Córdoba (Argentina), and, to learn about the natural habit and fungal associations in the Chaco Serrano woodland pristine region. In this study, bloomed orchid root and rhizosphere soil samples were obtained in two times from Valle de Punilla during spring of 2007; samples were kept in plastic bags until processed within 48 hours, and mycorrhizal condition confirmed assessing peloton presence. A total of 23 isolates of the orchideous mycorrhizal Rhizoctonia complex were obtained. The isolates were studied based on morphological characters and ITS-rDNA sequences. Morphological characteristics as color of colonies, texture, growth rate, hyphal diameter and length and presence of sclerotia were observed on culture media. To define the number of nuclei per cell, the isolates were grown in Petri dishes containing water-agar (WA) for three days at 25°C and stained with Safranine-O solution. The mycorrhizal fungi were grouped into binucleate (MSGib, 10 isolates) and multinucleate (MSGim, 13 isolates) based on morphological characteristics of the colonies. We obtained the ITS1-5.8s-ITS4 region that was amplified using primers ITS1 and ITS4. Based on DNA sequencing, isolates Q23 and Q29 were found to be related to species of Ceratobasidium. Isolates Q24 and Q4 were related to the binucleated anastomosis group AG-C of Rhizoctonia sp. The rest of the isolates grouped in the Ceratobasidium clade without grouping. From our knowledge this is the first report of the association of the AG-C testers with terrestrial orchids. A high specificity was observed in the symbiotic relationship. As the mycorrhizal fungal isolates were obtained from native orchids, they could be incorporated in conservation programes of endangered orchids in Argentina.
- Published
- 2015
4. Fidelidad del asegurado usando técnicas de machine learning
- Author
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Fernández Di Stefano, Patricia, Universitat de Barcelona. Departament d'Econometria, Estadística i Economia Aplicada, Bolance Losilla, Catalina, and Bolancé Losilla, Catalina
- Subjects
Artificial intelligence ,Customer loyalty ,Fidelització dels clients ,Ciencias actuariales ,Actuarial science ,68T Artificial intelligence ,68W Algorithms ,Estadística ,Máquinas de soporte vectorial ,Retencion del asegurado ,Artificial neural networks (ANN) ,Client retention ,Modelos estadísticos ,Support vector machines (SVM) ,Machine learning ,Aprenentatge automàtic ,Aprendizaje automatizado ,Bachelor's thesis ,Intel·ligència artificial ,Bachelor's theses ,Statistics ,Matemàtiques i estadística [Àrees temàtiques de la UPC] ,Treballs de fi de grau ,Inteligencia artificial ,Fidelización del cliente ,Statistical models ,Redes neuronales artificiales ,Customer loyalty programs - Abstract
Treballs Finals de Grau en Estadística UB-UPC, Facultat d'Economia i Empresa (UB) i Facultat de Matemàtiques i Estadística (UPC), Curs: 2020-2021, Tutor: Catalina Bolance Losilla, Se utilizaron métodos de machine learning, concretamente las NN-neural networks (redes neuronales) y el SVM support vector machine (máquina de soporte vectorial) para entrenar modelos con el objetivos de predecir la probabilidad de que un asegurado cancele su poliza de seguros de auto u hogar. También se estudia si la siniestralidad guarda relacion con esta cancelación. Los resultados obtenidos en esta investigación ponen de manifiesto como las NN se pueden adaptar mejor a la problemática de la desproporción del estado de las pólizas, ya que las SVM tienden a favorecer a las pólizas vigentes por ser la clase mayoritaria. También se evidencia la importancia de la siniestralidad, las características contractuales y su histórico a la hora de modelizar la predicción de cancelar la póliza.
- Published
- 2021
5. Fidelidad del asegurado usando técnicas de machine learning
- Author
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Universitat de Barcelona. Departament d'Econometria, Estadística i Economia Aplicada, Bolance Losilla, Catalina, Fernández Di Stefano, Patricia, Universitat de Barcelona. Departament d'Econometria, Estadística i Economia Aplicada, Bolance Losilla, Catalina, and Fernández Di Stefano, Patricia
- Abstract
Se utilizaron métodos de machine learning, concretamente las NN-neural networks (redes neuronales) y el SVM support vector machine (máquina de soporte vectorial) para entrenar modelos con el objetivos de predecir la probabilidad de que un asegurado cancele su poliza de seguros de auto u hogar. También se estudia si la siniestralidad guarda relacion con esta cancelación. Los resultados obtenidos en esta investigación ponen de manifiesto como las NN se pueden adaptar mejor a la problemática de la desproporción del estado de las pólizas, ya que las SVM tienden a favorecer a las pólizas vigentes por ser la clase mayoritaria. También se evidencia la importancia de la siniestralidad, las características contractuales y su histórico a la hora de modelizar la predicción de cancelar la póliza., Machine learning methods were used, specifically NN (neural networks) and SVM (support vector machine) to train models with the objective of predicting the probability that an client will cancel their auto or home insurance policy. It is also studied whether the claims rate is related to this cancellation. The results of this research show how the NN can be better adapted to the problem of the disproportion of the state of the policies, since the SVM favors the not canceled policies because they are the majority class. The importance of repoting claims, the contractual characteristics and its history are also evidence when modeling the prediction of canceling the policy.
- Published
- 2021
6. Diagnose of Indigenous Arbuscular Mycorrhizal Communities Associated to Cynara cardunculus L. var. altilis and var. sylvestris
- Author
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Micaela Mancini, María Lourdes Gil-Cardeza, Vanina Cravero, and Agustina Fernández Di Pardo
- Subjects
0303 health sciences ,Rhizosphere ,Soil salinity ,030306 microbiology ,Intensive farming ,Cynara ,General Medicine ,Biology ,Applied Microbiology and Biotechnology ,Microbiology ,Soil quality ,Plant Roots ,03 medical and health sciences ,Soil ,Agronomy ,Symbiosis ,Mycorrhizae ,Microbial inoculant ,Plant nutrition ,Mycelium ,Soil Microbiology ,030304 developmental biology - Abstract
Cynara cardunculus L. is a perennial species with high potential for bioenergy production. Arbuscular mycorrhizal symbiosis (AMF) is probably the terrestrial symbiosis most extended on earth. It presence in roots and soils improves plant nutrition and soil quality. Indigenous AMF have developed a variety of modifications to survive in their habitat and thus could serve as potential inoculants for the implantation of plant species in the respective AMF soil habitat. This work aimed to diagnose the status of the AMF symbiosis associated to two cardoon cultivars after a year of growth in a saline soil and in a conventional farming soil. For that purpose we determined AMF parameters in 4 rhizospheric soils and in roots of the cardoon varieties. We found that: (1) the rhizosphere of C. cardunculus var. altilis positively influenced the extraradical mycelium development in the saline soil, (2) the inorganic fertilization history of the conventional farming soil could have had a negative effect on the AMF community and, (3) the intraradical mycelium (IRM) development was extremely low. Our diagnosis suggests that, in order to improve the positive effects of AMF on cardoon growth and soil quality, efforts should be focused on the development of the IRM. In a boarder sense, the implementation of a diagnosis of indigenous AMF communities as a general agronomic practice could become an useful tool to farmers that are willing to potentiate the benefits of AMF on plant growth and soil quality.
- Published
- 2020
7. Diagnose of Indigenous Arbuscular Mycorrhizal Communities Associated to Cynara cardunculus L. var. altilis and var. sylvestris
- Author
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Fernández Di Pardo, Agustina, primary, Mancini, Micaela, additional, Cravero, Vanina, additional, and Gil-Cardeza, María Lourdes, additional
- Published
- 2020
- Full Text
- View/download PDF
8. Knowledge, Attitude, and Practice Toward Cervical Cancer Screening Among Female University Students in Ishaka Western Uganda
- Author
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Usman IM, Chama N, Aigbogun EO Jr, Kabanyoro A, Kasozi KI, Usman CO, Fernandez Diaz ME, Ndyamuhakyi E, Archibong VB, Onongha C, Ochieng JJ, Kanee RB, and Ssebuufu R
- Subjects
cervical cancer screening ,human papillomavirus ,university students ,uganda cancer control ,Gynecology and obstetrics ,RG1-991 - Abstract
Ibe Michael Usman,1 Naguledaticha Chama,2 Eric O Aigbogun Jr,3 Annet Kabanyoro,4 Keneth Iceland Kasozi,5 Comfort Ojochenemi Usman,6 Mario Edgar Fernandez Diaz,1 Elisa Ndyamuhakyi,1 Victor Bassey Archibong,7 Comfort Onongha,4 Juma John Ochieng,1 Rogers Bariture Kanee,8 Robinson Ssebuufu9 1Department of Human Anatomy, Kampala International University Western Campus, Bushenyi, Uganda; 2Department of Surgery, Kampala International University Teaching Hospital, Ishaka, Uganda; 3Department of Human Anatomy, Enugu State University of Science and Technology, Enugu, Nigeria; 4Department of Nursing, Aga Khan University, Kampala, Uganda; 5Physiology Department, Kabale University, Kabale, Uganda; 6Nursing Department, Ahmadu Bello University Teaching Hospital, Tudun Wada, Nigeria; 7Department of Human Anatomy, University of Rwanda, Kigali, Rwanda; 8Institute of Geo-Science and Space Technology, Rivers State University, Port Harcourt, Nigeria; 9Department of Surgery, Mengo Hospital, Kampala, UgandaCorrespondence: Ibe Michael Usman, Department of Human Anatomy, Kampala International University Western Campus, Bushenyi, Uganda, Tel +256706666798, Email gopama13@gmail.comPurpose: Cervical cancer (CC) is the leading cause of cancer-related death among women living in third-world countries. CC is preventable, with the possibility of complete treatment if detected early. The objective of the study was to assess the level of knowledge, attitude, and practice on CC screening (CCS) among female university students (FUS) in Ishaka, western Uganda.Methods: The study was a descriptive cross-sectional study among FUS (n = 407) in western Uganda conducted after the first nationwide lockdown in Uganda. Information was collected using a questionnaire and descriptively presented as frequency and percentages.Results: The majority of our respondents were medical students 283 (69.5%), below 25 years 339 (83.3%), with Anglican Christian religious background 150 (36.9%). Respondents above the age of 25 years (p = 0.0052) and those in the medical profession (p < 0.001) had more knowledge. More medical students had a better attitude (p = 0.0043) and favorable practices (0.0134) compared to their non-medical counterparts. There is a weak correlation between attitude (r = 0.206, p < 0.001) and practice (r = 0.181, p = 0.0003) with knowledge on CCS.Conclusion: Observation from the present study suggests the need for more efforts in the fight against cervical cancer and encouragement of positive attitude and practice towards cervical cancer screening and uptake of vaccination.Keywords: cervical cancer screening, human papillomavirus, university students, Uganda cancer control
- Published
- 2023
9. Dark septate endophytes present different potential to solubilize calcium, iron and aluminum phosphates
- Author
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N. Tobar, Federico N. Spagnoletti, Viviana Monica Chiocchio, Raúl S. Lavado, and A. Fernández Di Pardo
- Subjects
0106 biological sciences ,Biofertilizer ,Biotecnología del Medio Ambiente ,Soil Science ,chemistry.chemical_element ,INGENIERÍAS Y TECNOLOGÍAS ,01 natural sciences ,chemistry.chemical_compound ,Alkali soil ,Ophiosphaerella ,Botany ,Iron phosphate ,Cochliobolus ,Bioremediación, Diagnóstico Biotecnológico en Gestión Medioambiental ,Ecology ,biology ,Phosphorus ,BIOFERTILIZERS ,ENDOPHYTE FUNGI ,04 agricultural and veterinary sciences ,biology.organism_classification ,Phosphate ,Agricultural and Biological Sciences (miscellaneous) ,SOIL PHOSPHATES ,Chloris gayana ,chemistry ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,010606 plant biology & botany ,SOIL FUNGI - Abstract
Many microorganisms play a significant role in releasing phosphorus (P) from soil insoluble phosphates to crops. Here, we evaluated the ability of dark septate endophytes (DSE) to solubilize calcium, aluminum and iron phosphates. DSE were isolated from the roots of wheat (Triticum aestivum) and the forages Panicum coloratum and Chloris gayana, which are grown in slightly acidic and alkaline soils of the Argentine Pampas, respectively. Protocols to corroborate their endophytic nature were followed. Nine fungi were identified by morphological and molecular characteristics, and their sequences were deposited in GenBank. The isolates belonged to the same order and genera as DSE fungi recorded in other parts of the world. The temperature and pH requirements of the DSE strains were verified. To determine their ability to solubilize phosphate, we followed two in vitro methodologies: solid and liquid media. On solid medium, all isolates showed ability to solubilize calcium phosphate, three strains solubilized aluminum phosphate, and none of them solubilized iron phosphate. The DSE most efficient in solubilizing calcium phosphate were Ophiosphaerella sp. and Cochliobolus sp., followed by Setosphaeria rostrata. The strains Drechslera sp. (P6), Ophiosphaerella herpotricha and Drechslera sp. (12–15) were able to solubilize aluminum phosphate. In liquid medium, the isolates showed different ability to generate acidity and to solubilize phosphates. Drechslera sp. (12–15) was among the most efficient in solubilizing calcium phosphate, Curvularia sp. in solubilizing aluminum phosphate and Ophiosphaerella sp. in solubilizing iron phosphate. The results obtained combining both methodologies indicate that S. rostrata was not the best with each phosphate individually but showed the best global performance. DSE fungi are far less identified than other groups of fungi and bacteria as soil insoluble phosphate-solubilizing agents. However, they showed potential for application as biofertilizers in different soils to manage sustainable agroecosystems. Fil: Spagnoletti, Federico Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina Fil: Tobar Gomez, Natalia Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina Fil: Fernández Di Pardo, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina Fil: Chiocchio, Viviana Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina Fil: Lavado, Raul Silvio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
- Published
- 2017
10. Dark septate endophytes present different potential to solubilize calcium, iron and aluminum phosphates
- Author
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Spagnoletti, F.N., primary, Tobar, N.E., additional, Fernández Di Pardo, A., additional, Chiocchio, V.M., additional, and Lavado, R.S., additional
- Published
- 2017
- Full Text
- View/download PDF
11. Mycorrhizal fungi isolated from native terrestrial orchids of pristine regions in Córdoba (Argentina)
- Author
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Fernández Di Pardo, A., Chiocchio, V. M., Barrera, V. A., Colombo, R. P., Martinez, A. E., Gasoni, L., and Godeas, A. M.
- Subjects
TERRESTRIAL ORCHIDS ,MYCORRHIZA ,ITS - RDNA ,RHIZOCTONIA ,fungi ,EPULORHIZA - Abstract
275-283 Orchidaceae is a highly dependent group on the Rhizoctonia complex that includes Ceratorhiza, Moniliopsis, Epulorhiza and Rhizoctonia, for seed germination and the development of new orchid plants. Thus, the isolation and identification of orchid mycorrhizal fungi are important to understand the orchid-fungus relationship, which can lead to the development of efficient conservation strategies by in vivo germination of seeds from endangered orchid plants. The aim of our work was to isolate and characterize the different mycorrhizal fungi found in roots of terrestrial orchids from Córdoba (Argentina), and, to learn about the natural habit and fungal associations in the Chaco Serrano woodland pristine region. In this study, bloomed orchid root and rhizosphere soil samples were obtained in two times from Valle de Punilla during spring of 2007; samples were kept in plastic bags until processed within 48 hours, and mycorrhizal condition confirmed assessing peloton presence. A total of 23 isolates of the orchideous mycorrhizal Rhizoctonia complex were obtained. The isolates were studied based on morphological characters and ITS-rDNA sequences. Morphological characteristics as color of colonies, texture, growth rate, hyphal diameter and length and presence of sclerotia were observed on culture media. To define the number of nuclei per cell, the isolates were grown in Petri dishes containing water-agar (WA) for three days at 25°C and stained with Safranine-O solution. The mycorrhizal fungi were grouped into binucleate (MSGib, 10 isolates) and multinucleate (MSGim, 13 isolates) based on morphological characteristics of the colonies. We obtained the ITS1-5.8s-ITS4 region that was amplified using primers ITS1 and ITS4. Based on DNA sequencing, isolates Q23 and Q29 were found to be related to species of Ceratobasidium. Isolates Q24 and Q4 were related to the binucleated anastomosis group AG-C of Rhizoctonia sp. The rest of the isolates grouped in the Ceratobasidium clade without grouping. From our knowledge this is the first report of the association of the AG-C testers with terrestrial orchids. A high specificity was observed in the symbiotic relationship. As the mycorrhizal fungal isolates were obtained from native orchids, they could be incorporated in conservation programes of endangered orchids in Argentina. Rev. Biol. Trop. 63 (1) : 275-283. Epub 2015 March 01.
- Published
- 2015
12. Soils of Buenos Aires Province contaminated with organophosphate herbicide (glyphosate) : impact on soil filamentous fungi community
- Author
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Fernández Di Pardo, Agustina and Godeas, Alicia
- Subjects
SOIL CONTAMINATION ,GLYPHOSATE ,FILAMENTOUS FUNGI - Abstract
El herbicida más utilizado en Argentina es el N-phosphonomethylglycine (nombre comercial: Glifosato). Éste puede ser rápida y completamente degradado por los microorganismos, por lo que eltiempo de vida en el suelo es bajo. Sin embargo, el comportamiento del glifosato puede variar enfunción de las características físicas y químicas del suelo sobre el que se aplique. Por lo tanto, eltiempo de permanencia en el suelo podría ser mayor. Es importante estudiar el comportamiento deeste herbicida dada las potenciales alteraciones que puede provocar en las comunidades demicroorganismos que viven en el suelo y en algunos de sus procesos. La contaminación de los suelosno sólo está afectando su biodiversidad microbiana sino también a su fertilidad, dado que losmicroorganismos son fundamentales para el proceso de reciclaje de nutrientes. En este trabajo, se compararon comunidades de hongos filamentosos provenientes desuelos con y sin historia de aplicación de glifosato y se evaluó la capacidad de los hongos filamentososen la recuperación de suelos contaminados por el herbicida glifosato. Para ello, se aislaron ycaracterizaron hongos filamentosos del suelo en dos sitios con distinta historia de aplicación deglifosato utilizando dos técnicas distintas (método tradicional de lavado de muestras múltiples ysiembra de partículas de suelo y el método de pirosecuenciación). Se seleccionaron cepas tolerantes aglifosato, se estudiaron los efectos del glifosato sobre los hongos luego de una aplicación puntual y seevaluó la presencia de glifosato en el suelo. Se observó que las poblaciones fúngicas del suelo varían en su composición específica y en subiomasa en suelos expuestos a glifosato en comparación con suelos sin exposición. La mayor riquezaespecífica y los mayores valores de ergosterol, como medida indirecta de biomasa, se obtuvieron ensuelos sin historia de prácticas agrícolas. Se lograron obtener cepas tolerantes al glifosato: T.harzianum; F. oxysporum; P. lilacinus; C. didymum. Finalmente, los valores de concentración deglifosato en suelos fueron bajos y prácticamente nulos luego de 3 meses de la aplicación en la mayoríade los casos. The most widely used herbicide in Argentina in de N-phosphonomethylglycine (glyphosate). Because it can be rapidly and completely degraded by microorganisms, itdisappears quickly from soil. However, the destiny of glyphosate in soil may varydepending on the physical and chemical soil characteristics. It is important to study thebehavior of this herbicide given the potential changes that may produce in thecommunities of microorganisms and in the processes that involves them. The soilcontamination with this herbicide no only affects the microbial biodiversity, but also itsfertility, considering that microorganisms are a fundamental part of the nutrientrecycling process in soil. In this study, changes in filamentous fungi communities’ from soils that werepreviously or not exposed to the herbicide and the capacity of some strains ofremediating contaminated soils were evaluated. Soil fungi were isolated andcommunities were characterizes in two sites with different history of exposure toglyphosate, using two techniques: traditional isolation method (morphological) andpyrosequencing (molecular). Tolerant strains of fungi were selected; effects of recentglyphosate application on soils fungal communities was evaluated and remnantglyphosate in soil was quantified. In conclusion, soil fungal community changes and fungal biomass reductionwere observed after glyphosate application. Six tolerant strains were obtained: T.harzianum; F. oxysporum; P. lilacinus; C. didymum. The concentration of glyphosate insoil was practically zero after 3 month from application. Fil: Fernández Di Pardo, Agustina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
- Published
- 2014
13. Efecto diferencial de dos cepas de Rhizophagus intraradices sobre la biomasa y el rendimiento y composición de aceites esenciales de Calamintha nepeta
- Author
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Agustina Fernández Di Pardo, Laura Fernández Bidondo, Alicia Elba Martinez, Paola Di Leo Lira, Roxana Colombo, Alicia Margarita Godeas, and Catalina van Baren
- Subjects
Microbiology (medical) ,ARBUSCULAR MYCORRHIZAL SIMBIOSIS ,lcsh:QR1-502 ,Rendimiento de aceites esenciales ,Biomass ,chemistry.chemical_element ,ESSENTIAL OIL COMPOSITION ,Microbiology ,lcsh:Microbiology ,Calamintha ,lcsh:Infectious and parasitic diseases ,law.invention ,purl.org/becyt/ford/1 [https] ,Ciencias Biológicas ,Human fertilization ,law ,Nepeta ,Agronomía, reproducción y protección de plantas ,Botany ,Calamintha nepeta ,Oils, Volatile ,lcsh:RC109-216 ,Colonization ,CALAMINTHA NEPETA ,purl.org/becyt/ford/1.6 [https] ,Glomeromycota ,ESSENTIAL OIL YIELD ,Essential oil ,biology ,Phosphorus ,food and beverages ,General Medicine ,Simbiosis micorrícica arbuscular ,Arbuscular mycorrhizal symbiosis ,biology.organism_classification ,Essential oil yield ,Horticulture ,chemistry ,CIENCIAS AGRÍCOLAS ,purl.org/becyt/ford/4.1 [https] ,Essential oil composition ,Composition (visual arts) ,Micología ,Agricultura, Silvicultura y Pesca ,Composición de aceites esenciales ,purl.org/becyt/ford/4 [https] ,CIENCIAS NATURALES Y EXACTAS - Abstract
El objetivo de este trabajo fue determinar, bajo condiciones de invernadero, el efecto de dos cepas geográficamente diferentes de Rhizophagus intraradices (M3 y GA5) sobre la biomasa total y el rendimiento y composición de aceites esenciales (AE) de Calamintha nepeta, con fertilización fosforada (P) o sin esta. La biomasa de la planta no fue significativamente afectada por ningún tratamiento, y se observaron valores más altos en las plantas control. Las cepas mostraron diferencias en sus tasas de colonización y en las respuestas a la fertilización con fósforo: M3 redujo sus valores de colonización, mientras que GA5 no los modificó. En ausencia de fertilización fosforada, las plantas colonizadas por ambas cepas presentaron rendimientos de AE diferentes a aquellos de las plantas control: M3 los aumentó y GA5 los disminuyó. La composición porcentual de AE no fue modificada significativamente por ninguno de los tratamientos. M3 podría ser considerada como un posible bioinoculante fúngico para la producción de C. nepeta destinada al mercado de las plantas aromáticas. The aim of this work was to determine the effects of two geographically different strains of Rhizophagus intraradices (M3 and GA5) on the total biomass and essential oil (EO) yield and composition of Calamintha nepeta, with or without phosphorus (P) fertilization, under greenhouse conditions.The plant biomass was not significantly affected by any of the treatments, showing higher values in control plants. Strains had a differential response in their root colonization rates: M3 reduced these parameters while GA5 did not modify them. Both strains affected EO yield in absence of P fertilization: M3 promoted EO yield in C. nepeta plants and GA5 resulted in negative effects. The percentage composition of EO was not significantly modified by either strain or P fertilization. M3 strain could be a potential fungal bioinoculant for production and commercialization of C. nepeta in the aromatic plant market. Fil: Colombo, Roxana. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biologia Experimental; Argentina Fil: Martinez, Alicia Elba. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biologia Experimental; Argentina Fil: Fernández Di Pardo, Agustina. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biologia Experimental; Argentina Fil: Fernandez Bidondo, Laura. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biologia Experimental; Argentina Fil: Van Baren, Catalina Maria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquimica. Instituto de Química y Metabolismo del Fármaco; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco (i); Argentina Fil: Di Leo Lira, Paola Maria del Rosario. Universidad de Buenos Aires. Facultad de Farmacia y Bioquimica. Instituto de Química y Metabolismo del Fármaco; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco (i); Argentina Fil: Godeas, Alicia Margarita. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biologia Experimental; Argentina
- Published
- 2013
14. Mycorrhizal fungi isolated from native terrestrial orchids of pristine regions in Córdoba (Argentina)
- Author
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Fernández Di Pardo, Agustina, primary, Chiocchio, Viviana M., additional, Barrera, Viviana, additional, Colombo, Roxana P., additional, Gasoni, Laura, additional, and Martinez, Alicia E., additional
- Published
- 2015
- Full Text
- View/download PDF
15. Differential effects of two strains of Rhizophagus intraradices on dry biomass and essential oil yield and composition in Calamintha nepeta
- Author
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Colombo, Roxana P., primary, Martínez, Alicia E., additional, Pardo, Agustina Fernández di, additional, Bidondo, Laura Fernández, additional, Baren, Catalina van, additional, Lira, Paola di Leo, additional, and Godeas, Alicia M., additional
- Published
- 2013
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16. Devices for Prevention of Atrial Tachyarrhythmias
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Fernández Lozano, Ignacio, Toquero, Jorge, Fernández Díaz, José Antonio, Ionescu, Bogdan, Moñivas, Vanesa, Ortiz, Pilar, Fuertes, Beatriz, Singh, Balbir, Lokhandwala, Yash, Francis, Johnson, and Gupta, Anup
- Subjects
JOURNALS: Indian Pacing and Electrophysiology Journal ,Indian Pacing and Electrophysiology Journal - Abstract
Atrial fibrillation (AF) is the most frequent sustained cardiac arrhythmia in clinical practice and, although its importance has been underestimated even in recent years, we are now becoming aware of its clinical transcendence1,2,3. The classical treatment is pharmacological, but its efficacy is limited and it does have side effects4,5. Therefore, in recent years, there has been an increasing interest in other types of non-pharmacological treatments6,7. Physiologic cardiac pacing has proven to be more effective than VVI mode pacing to prevent the occurrence of AF during the follow-up of patients who have had a permanent pacemaker implanted 8,9,10. There are currently different lines of research that use different atrial pacing techniques to prevent and treat episodes of paroxysmal atrial fibrillation11,12. Techniques of multi-site pacing in the right atrium or both atria, new atrial pacing sites, prevention algorithms for paroxysmal atrial fibrillation episodes, and even high-frequency atrial tachyarrhythmia termination algorithms have all been proposed. In this article, we will try to synthesize the grounds for and findings of the different lines of research currently being developed.
- Published
- 2004
17. In situ AFM observations of mineral replacement reactions on sulphate and carbonate surfaces
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Pina, C.M., Sánchez-Pastor, N., Fernández-Dı´az, L., Pérez-Garrido, C., and Prieto, M.
- Published
- 2006
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18. Las micorrizas arbusculares y Rhizobium: una simbiosis dual de interés
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Viviana Monica Chiocchio, Agustina Fernández Di Pardo, Natalia Elizabeth Tobar Gomez, and Federico N. Spagnoletti
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Microbiology (medical) ,SINORHIZOBIUM MELILOTI ,GLOMUS INTRARADICES ,lcsh:QR1-502 ,ALFALFA ,General Medicine ,Bioquímica y Biología Molecular ,Microbiology ,lcsh:Microbiology ,lcsh:Infectious and parasitic diseases ,purl.org/becyt/ford/1 [https] ,Ciencias Biológicas ,lcsh:RC109-216 ,purl.org/becyt/ford/1.6 [https] ,CIENCIAS NATURALES Y EXACTAS - Abstract
Los microorganismos benéfi cos juegan un rol fundamental en los agroecosistemas naturalmente sustentables. Algunos de ellos pueden ser utilizados como inoculantes para bene- fi ciar a las plantas, ya que desarrollan actividades que involucran una promoción de su crecimiento y su protección. Las asociaciones de Rhizobium-micorrizas arbusculares (MA) actúan sinérgicamente en los niveles de infección, nutrición mineral y crecimiento de las plantas. Fil: Spagnoletti, Federico Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina Fil: Fernández Di Pardo, Agustina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tobar Gomez, Natalia Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina Fil: Chiocchio, Viviana Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
19. Identification and characterization of the Non-race specific Disease Resistance 1 (NDR1) orthologous protein in coffee
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Mongrand Sébastien, Estevan Joan, Conejero Geneviève, Bernier Louis, Petitot Anne-Sophie, Cacas Jean-Luc, and Fernandez Diana
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Botany ,QK1-989 - Abstract
Abstract Background Leaf rust, which is caused by the fungus Hemileia vastatrix (Pucciniales), is a devastating disease that affects coffee plants (Coffea arabica L.). Disadvantages that are associated with currently developed phytoprotection approaches have recently led to the search for alternative strategies. These include genetic manipulations that constitutively activate disease resistance signaling pathways. However, molecular actors of such pathways still remain unknown in C. arabica. In this study, we have isolated and characterized the coffee NDR1 gene, whose Arabidopsis ortholog is a well-known master regulator of the hypersensitive response that is dependent on coiled-coil type R-proteins. Results Two highly homologous cDNAs coding for putative NDR1 proteins were identified and cloned from leaves of coffee plants. One of the candidate coding sequences was then expressed in the Arabidopsis knock-out null mutant ndr1-1. Upon a challenge with a specific strain of the bacterium Pseudomonas syringae (DC3000::AvrRpt2), analysis of both macroscopic symptoms and in planta microbial growth showed that the coffee cDNA was able to restore the resistance phenotype in the mutant genetic background. Thus, the cDNA was dubbed CaNDR1a (standing for Coffea arabica Non-race specific Disease Resistance 1a). Finally, biochemical and microscopy data were obtained that strongly suggest the mechanistic conservation of the NDR1-driven function within coffee and Arabidopsis plants. Using a transient expression system, it was indeed shown that the CaNDR1a protein, like its Arabidopsis counterpart, is localized to the plasma membrane, where it is possibly tethered by means of a GPI anchor. Conclusions Our data provide molecular and genetic evidence for the identification of a novel functional NDR1 homolog in plants. As a key regulator initiating hypersensitive signalling pathways, CaNDR1 gene(s) might be target(s) of choice for manipulating the coffee innate immune system and achieving broad spectrum resistance to pathogens. Given the potential conservation of NDR1-dependent defense mechanisms between Arabidopsis and coffee plants, our work also suggests new ways to isolate the as-yet-unidentified R-gene(s) responsible for resistance to H. vastatrix.
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- 2011
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20. Pintura mural de la villa romana de Balazote (Albacete)
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Fernández Díaz, Alicia
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Prehistoric archaeology ,GN700-890 ,Archaeology ,CC1-960 - Abstract
Con este estudio pretendemos sacar a la luz un importante conjunto de pintura mural procedente de la villa romana de Balazote (Albacete), de la que hasta ahora se habían realizado un gran número de trabajos, incluidos los relativos a su decoración musiva, pero en los que no se había tratado de forma más completa el tema de los revestimientos pictóricos de sus paredes. La totalidad de los fragmentos procede del área termal y su importancia radica principalmente en ser uno de los pocos ejemplos de decoración figurada con representaciones de palestra que encontramos en la Península Ibérica y en el resto de las provincias romanas de Occidente.
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- 2003
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21. Algunos restos pictóricos de la ciudad de Lucentum (Tossal de Manises-Alicante)
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Fernández Díaz, Alicia
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Prehistoric archaeology ,GN700-890 ,Archaeology ,CC1-960 - Abstract
El presente artículo analiza la pintura mural hallada tras las excavaciones de la domus de la Puerta Oriental de Lucentum. Ésta corresponde a la estancia norte y más concretamente al rodapié, zócalo, zona media y registro superior o techo de las paredes norte y oeste. Los fragmentos y las grandes placas conservadas muestran una decoración típica dentro del panorama de la pintura romana provincial de los siglos I y II dC, donde el zócalo presenta imitaciones marmóreas; la zona media se desarrolla entre paneles anchos e interpaneles estrechos en cuyo interior figuran los característicos candelabros metálicos; un registro superior ornado por una gran cornisa moldurada en estuco y el techo, donde los motivos decorativos aparecen ejecutados sobre un sistema de red.
- Published
- 2001
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22. Química general e inorgánica
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Liberatti, Ana María, Mancini, Micaela, Mosconi, Natalia Ester, Giuntoli, Gustavo, Almirón Chamadoira, Paula Gabriela, Lazzarini, Agustina, Priotti, Josefina, Amato, Lucía Dolores, Ranea, Mariana, Teixeido, Matías, Arrizabalaga, Gonzalo, Punschke, Eduardo Luján, Vázquez, Juan Manuel, and Fernández Di Matteo, Gabriela
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CIENCIAS AGRÍCOLAS ,QUÍMICA ,Otras Ciencias Agrícolas ,MATEMÁTICA ,purl.org/becyt/ford/4.5 [https] ,FÍSICA ,purl.org/becyt/ford/4 [https] - Abstract
La química suele considerarse como una materia difícil y compleja. En cierto sentido esto es justificable por un motivo; la química tiene un vocabulario muy especializado. Es por eso que muchas veces estudiar química es como aprender un nuevo idioma. Además, algunos de sus conceptos son abstractos. Sin embargo, también podemos decir de ella que es una ciencia básica, que se mueve entre lo empírico y lo teórico, entre una naturaleza repleta de singularidades y el reino de las leyes generales, entre la asimetría y la armonía. Los invitamos a recorrer juntos este camino de enseñanza y aprendizaje que seguramente los llevará al descubrimiento de una ciencia fundamental y desafiante cuyos contenidos lo acompañarán a lo largo de su camino como estudiante y futuros profesionales. Fil: Liberatti, Ana María. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina Fil: Mancini, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina Fil: Mosconi, Natalia Ester. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina Fil: Giuntoli, Gustavo. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina Fil: Almirón Chamadoira, Paula Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina Fil: Lazzarini, Agustina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina Fil: Priotti, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina Fil: Amato, Lucía Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina Fil: Ranea, Mariana. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina Fil: Teixeido, Matías. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina
- Published
- 2019
23. Differential effects of two strains of Rhizophagus intraradices on dry biomass and essential oil yield and composition in Calamintha nepeta
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Roxana P Colombo, Alicia E Martínez, Agustina Fernández di Pardo, Laura Fernández Bidondo, Catalina van Baren, Paola di Leo Lira, and Alicia M Godeas
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Simbiosis micorrícica arbuscular ,Calamintha nepeta ,Rendimiento de aceites esenciales ,Composición de aceites esenciales ,Infectious and parasitic diseases ,RC109-216 ,Microbiology ,QR1-502 - Abstract
The aim of this work was to determine the effects of two geographically different strains of Rhizophagus intraradices (M3 and GA5) on the total biomass and essential oil (EO) yield and composition of Calamintha nepeta, with or without phosphorus (P) fertilization, under greenhouse conditions.The plant biomass was not significantly affected by any of the treatments, showing higher values in control plants. Strains had a differential response in their root colonization rates: M3 reduced these parameters while GA5 did not modify them. Both strains affected EO yield in absence of P fertilization: M3 promoted EO yield in C. nepeta plants and GA5 resulted in negative effects. The percentage composition of EO was not significantly modified by either strain or P fertilization. M3 strain could be a potential fungal bioinoculant for production and commercialization of C. nepeta in the aromatic plant market.
24. Ultra-high throughput-based screening for the discovery of antiplatelet drugs affecting receptor dependent calcium signaling dynamics.
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Fernández DI, Troitiño S, Sobota V, Tullemans BME, Zou J, van den Hurk H, García Á, Honarnejad S, Kuijpers MJE, and Heemskerk JWM
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- Humans, Thrombin metabolism, Calcium Signaling, Platelet Membrane Glycoproteins metabolism, Receptor, PAR-1 metabolism, Blood Platelets metabolism, Platelet Activation, Calcium, Dietary pharmacology, Platelet Aggregation, Platelet Aggregation Inhibitors pharmacology, Calcium metabolism, Phenothiazines
- Abstract
Distinct platelet activation patterns are elicited by the tyrosine kinase-linked collagen receptor glycoprotein VI (GPVI) and the G-protein coupled protease-activated receptors (PAR1/4) for thrombin. This is reflected in the different platelet Ca
2+ responses induced by the GPVI agonist collagen-related peptide (CRP) and the PAR1/4 agonist thrombin. Using a 96 well-plate assay with human Calcium-6-loaded platelets and a panel of 22 pharmacological inhibitors, we assessed the cytosolic Ca2+ signaling domains of these receptors and developed an automated Ca2+ curve algorithm. The algorithm was used to evaluate an ultra-high throughput (UHT) based screening of 16,635 chemically diverse small molecules with orally active physicochemical properties for effects on platelets stimulated with CRP or thrombin. Stringent agonist-specific selection criteria resulted in the identification of 151 drug-like molecules, of which three hit compounds were further characterized. The dibenzyl formamide derivative ANO61 selectively modulated thrombin-induced Ca2+ responses, whereas the aromatic sulfonyl imidazole AF299 and the phenothiazine ethopropazine affected CRP-induced responses. Platelet functional assays confirmed selectivity of these hits. Ethopropazine retained its inhibitory potential in the presence of plasma, and suppressed collagen-dependent thrombus buildup at arterial shear rate. In conclusion, targeting of platelet Ca2+ signaling dynamics in a screening campaign has the potential of identifying novel platelet-inhibiting molecules., (© 2024. The Author(s).)- Published
- 2024
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25. Endothelium-mediated regulation of platelet activation: Involvement of multiple protein kinases.
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Provenzale I, Solari FA, Schönichen C, Brouns SLN, Fernández DI, Kuijpers MJE, van der Meijden PEJ, Gibbins JM, Sickmann A, Jones C, and Heemskerk JWM
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- Humans, Protein Kinases metabolism, Nitric Oxide metabolism, Endothelial Cells metabolism, Platelet Activation physiology, Blood Platelets metabolism, Endothelium metabolism, Prostaglandins I, Platelet Membrane Glycoproteins metabolism, Thrombin metabolism
- Abstract
The endothelial regulation of platelet activity is incompletely understood. Here we describe novel approaches to find molecular pathways implicated on the platelet-endothelium interaction. Using high-shear whole-blood microfluidics, employing coagulant or non-coagulant conditions at physiological temperature, we observed that the presence of human umbilical vein endothelial cells (HUVEC) strongly suppressed platelet adhesion and activation, via the collagen receptor glycoprotein VI (GPVI) and the PAR receptors for thrombin. Real-time monitoring of the cytosolic Ca
2+ rises in the platelets indicated no major improvement of inhibition by prostacyclin or nitric oxide. Similarly under stasis, exposure of isolated platelets to HUVEC reduced the Ca2+ responses by collagen-related peptide (CRP-XL, GPVI agonist) and thrombin (PAR agonist). We then analyzed the label-free phosphoproteome of platelets (three donors), exposed to HUVEC, CRP-XL, and/or thrombin. High-resolution mass spectrometry gave 5463 phosphopeptides, corresponding to 1472 proteins, with good correlation between biological and technical replicates (R > .86). Stringent filtering steps revealed 26 regulatory pathways (Reactome) and 143 regulated kinase substrates (PhosphoSitePlus), giving a set of protein phosphorylation sites that was differentially (44) or similarly (110) regulated by HUVEC or agonist exposure. The differential regulation was confirmed by stable-isotope analysis of platelets from two additional donors. Substrate analysis indicated major roles of poorly studied protein kinase classes (MAPK, CDK, DYRK, STK, PKC members). Collectively, these results reveal a resetting of the protein phosphorylation profile in platelets exposed to endothelium or to conventional agonists and to endothelium-promoted activity of a multi-kinase network, beyond classical prostacyclin and nitric oxide actors, that may contribute to platelet inhibition., (© 2024 The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.)- Published
- 2024
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26. Restraining of glycoprotein VI- and integrin α2β1-dependent thrombus formation by platelet PECAM1.
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Jooss NJ, Diender MG, Fernández DI, Huang J, Heubel-Moenen FCJ, van der Veer A, Kuijpers MJE, Poulter NS, Henskens YMC, Te Loo M, and Heemskerk JWM
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- Humans, Blood Platelets, Glycoproteins, Collagen, Integrin alpha2beta1 genetics, Thrombosis genetics
- Abstract
The platelet receptors, glycoprotein VI (GPVI) and integrin α2β1 jointly control collagen-dependent thrombus formation via protein tyrosine kinases. It is unresolved to which extent the ITIM (immunoreceptor tyrosine-based inhibitory motif) receptor PECAM1 and its downstream acting protein tyrosine phosphatase PTPN11 interfere in this process. Here, we hypothesized that integrin α2β1 has a co-regulatory role in the PECAM1- and PTPN11-dependent restraint of thrombus formation. We investigated platelet activation under flow on collagens with a different GPVI dependency and using integrin α2β1 blockage. Blood was obtained from healthy subjects and from patients with Noonan syndrome with a gain-of-function mutation of PTPN11 and variable bleeding phenotype. On collagens with decreasing GPVI activity (types I, III, IV), the surface-dependent inhibition of PECAM1 did not alter thrombus parameters using control blood. Blockage of α2β1 generally reduced thrombus parameters, most effectively on collagen IV. Strikingly, simultaneous inhibition of PECAM1 and α2β1 led to a restoration of thrombus formation, indicating that the suppressing signaling effect of PECAM1 is masked by the platelet-adhesive receptor α2β1. Blood from 4 out of 6 Noonan patients showed subnormal thrombus formation on collagen IV. In these patients, effects of α2β1 blockage were counterbalanced by PECAM1 inhibition to a normal phenotype. In summary, we conclude that the suppression of GPVI-dependent thrombus formation by either PECAM1 or a gain-of-function of PTPN11 can be overruled by α2β1 engagement., (© 2024. The Author(s).)
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- 2024
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27. Crucial roles of red blood cells and platelets in whole blood thrombin generation.
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Sun S, Campello E, Zou J, Konings J, Huskens D, Wan J, Fernández DI, Reutelingsperger CPM, Ten Cate H, Toffanin S, Bulato C, de Groot PG, de Laat B, Simioni P, Heemskerk JWM, and Roest M
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- Humans, Thrombin metabolism, Phosphatidylserines, Annexin A5, Erythrocytes metabolism, Thrombosis, Coagulants, Anemia
- Abstract
Red blood cells (RBCs) and platelets contribute to the coagulation capacity in bleeding and thrombotic disorders. The thrombin generation (TG) process is considered to reflect the interactions between plasma coagulation and the various blood cells. Using a new high-throughput method capturing the complete TG curve, we were able to compare TG in whole blood and autologous platelet-rich and platelet-poor plasma to redefine the blood cell contributions to the clotting process. We report a faster and initially higher generation of thrombin and shorter coagulation time in whole blood than in platelet-rich plasma upon low concentrations of coagulant triggers, including tissue factor, Russell viper venom factor X, factor Xa, factor XIa, and thrombin. The TG was accelerated with increased hematocrit and delayed after prior treatment of RBC with phosphatidylserine-blocking annexin A5. RBC treatment with ionomycin increased phosphatidylserine exposure, confirmed by flow cytometry, and increased the TG process. In reconstituted blood samples, the prior selective blockage of phosphatidylserine on RBC with annexin A5 enhanced glycoprotein VI-induced platelet procoagulant activity. For patients with anemia or erythrocytosis, cluster analysis revealed high or low whole-blood TG profiles in specific cases of anemia. The TG profiles lowered upon annexin A5 addition in the presence of RBCs and thus were determined by the extent of phosphatidylserine exposure of blood cells. Profiles for patients with polycythemia vera undergoing treatment were similar to that of control subjects. We concluded that RBC and platelets, in a phosphatidylserine-dependent way, contribute to the TG process. Determination of the whole-blood hypo- or hyper-coagulant activity may help to characterize a bleeding or thrombosis risk., (© 2023 by The American Society of Hematology. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), permitting only noncommercial, nonderivative use with attribution. All other rights reserved.)
- Published
- 2023
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28. Role of SHP2 (PTPN11) in glycoprotein VI-dependent thrombus formation: Improved platelet responsiveness by the allosteric drug SHP099 in Noonan syndrome patients.
- Author
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Fernández DI, Diender M, Hermida-Nogueira L, Huang J, Veiras S, Henskens YMC, Te Loo MWM, Heemskerk JWM, Kuijpers MJE, and García Á
- Subjects
- Humans, Blood Platelets metabolism, Thromboplastin metabolism, Platelet Glycoprotein GPIIb-IIIa Complex metabolism, Collagen metabolism, Fibrin metabolism, Platelet Membrane Glycoproteins, Protein Tyrosine Phosphatase, Non-Receptor Type 11 metabolism, Noonan Syndrome drug therapy, Noonan Syndrome genetics, Noonan Syndrome metabolism, Thrombosis
- Abstract
Introduction: The protein tyrosine phosphatase SHP2 (PTPN11) is a negative regulator of glycoprotein VI (GPVI)-induced platelet signal under certain conditions. Clinical trials with derivatives of the allosteric drug SHP099, inhibiting SHP2, are ongoing as potential therapy for solid cancers. Gain-of-function mutations of the PTPN11 gene are observed in part of the patients with the Noonan syndrome, associated with a mild bleeding disorder. Assessment of the effects of SHP2 inhibition in platelets from controls and Noonan syndrome patients., Materials and Methods: Washed human platelets were incubated with SHP099 and stimulated with collagen-related peptide (CRP) for stirred aggregation and flow cytometric measurements. Whole-blood microfluidics assays using a dosed collagen and tissue factor coating were performed to assess shear-dependent thrombus and fibrin formation. Effects on clot formation were evaluated by thromboelastometry., Results: Pharmacological inhibition of SHP2 did not alter GPVI-dependent platelet aggregation under stirring, but it enhanced integrin αIIbβ3 activation in response to CRP. Using whole-blood microfluidics, SHP099 increased the thrombus buildup on collagen surfaces. In the presence of tissue factor and coagulation, SHP099 increased thrombus size and reduced time to fibrin formation. Blood from PTPN11-mutated Noonan syndrome patients, with low platelet responsiveness, after ex vivo treatment with SHP099 showed a normalized platelet function. In thromboelastometry, SHP2 inhibition tended to increase tissue factor-induced blood clotting profiles with tranexamic acid, preventing fibrinolysis., Conclusion: Pharmacological inhibition of SHP2 by the allosteric drug SHP099 enhances GPVI-induced platelet activation under shear conditions with a potential to improve platelet functions of Noonan syndrome patients., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2023
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29. Roles of Focal Adhesion Kinase PTK2 and Integrin αIIbβ3 Signaling in Collagen- and GPVI-Dependent Thrombus Formation under Shear.
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Huang J, Jooss NJ, Fernández DI, Sickmann A, García Á, Wichapong K, Dijkgraaf I, and Heemskerk JWM
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- Blood Platelets metabolism, Collagen metabolism, Focal Adhesion Kinase 1 metabolism, Focal Adhesion Protein-Tyrosine Kinases metabolism, Humans, Peptides metabolism, Peptides pharmacology, Platelet Activation, Platelet Adhesiveness, Platelet Aggregation, Platelet Membrane Glycoproteins metabolism, Platelet Glycoprotein GPIIb-IIIa Complex metabolism, Thrombosis metabolism
- Abstract
Glycoprotein (GP)VI and integrin αIIbβ3 are key signaling receptors in collagen-dependent platelet aggregation and in arterial thrombus formation under shear. The multiple downstream signaling pathways are still poorly understood. Here, we focused on disclosing the integrin-dependent roles of focal adhesion kinase (protein tyrosine kinase 2, PTK2), the shear-dependent collagen receptor GPR56 (ADGRG1 gene), and calcium and integrin-binding protein 1 (CIB1). We designed and synthetized peptides that interfered with integrin αIIb binding (pCIB and pCIBm) or mimicked the activation of GPR56 (pGRP). The results show that the combination of pGRP with PTK2 inhibition or of pGRP with pCIB > pCIBm in additive ways suppressed collagen- and GPVI-dependent platelet activation, thrombus buildup, and contraction. Microscopic thrombus formation was assessed by eight parameters (with script descriptions enclosed). The suppressive rather than activating effects of pGRP were confined to blood flow at a high shear rate. Blockage of PTK2 or interference of CIB1 no more than slightly affected thrombus formation at a low shear rate. Peptides did not influence GPVI-induced aggregation and Ca2+ signaling in the absence of shear. Together, these data reveal a shear-dependent signaling axis of PTK2, integrin αIIbβ3, and CIB1 in collagen- and GPVI-dependent thrombus formation, which is modulated by GPR56 and exclusively at high shear. This work thereby supports the role of PTK2 in integrin αIIbβ3 activation and signaling.
- Published
- 2022
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30. Structure-Based Cyclic Glycoprotein Ibα-Derived Peptides Interfering with von Willebrand Factor-Binding, Affecting Platelet Aggregation under Shear.
- Author
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Hrdinova J, Fernández DI, Ercig B, Tullemans BME, Suylen DPL, Agten SM, Jurk K, Hackeng TM, Vanhoorelbeke K, Voorberg J, Reutelingsperger CPM, Wichapong K, Heemskerk JWM, and Nicolaes GAF
- Subjects
- Animals, Binding Sites, Blood Platelets metabolism, Cells, Cultured, Horses, Humans, Microfluidics, Peptides metabolism, Protein Binding, Stress, Mechanical, von Willebrand Factor metabolism, Blood Platelets physiology, Peptides chemistry, Platelet Aggregation, Platelet Glycoprotein GPIb-IX Complex chemistry, von Willebrand Factor chemistry
- Abstract
The plasmatic von Willebrand factor (VWF) circulates in a compact form unable to bind platelets. Upon shear stress, the VWF A1 domain is exposed, allowing VWF-binding to platelet glycoprotein Ib-V-IX (GPIbα chain). For a better understanding of the role of this interaction in cardiovascular disease, molecules are needed to specifically interfere with the opened VWF A1 domain interaction with GPIbα. Therefore, we in silico designed and chemically synthetized stable cyclic peptides interfering with the platelet-binding of the VWF A1 domain per se or complexed with botrocetin. Selected peptides (26-34 amino acids) with the lowest-binding free energy were: the monocyclic mono- vOn Willebrand factoR-GPIbα InTerference (ORbIT) peptide and bicyclic bi-ORbIT peptide. Interference of the peptides in the binding of VWF to GPIb-V-IX interaction was retained by flow cytometry in comparison with the blocking of anti-VWF A1 domain antibody CLB-RAg35. In collagen and VWF-dependent whole-blood thrombus formation at a high shear rate, CLB-RAg35 suppressed stable platelet adhesion as well as the formation of multilayered thrombi. Both peptides phenotypically mimicked these changes, although they were less potent than CLB-RAg35. The second-round generation of an improved peptide, namely opt-mono-ORbIT (28 amino acids), showed an increased inhibitory activity under flow. Accordingly, our structure-based design of peptides resulted in physiologically effective peptide-based inhibitors, even for convoluted complexes such as GPIbα-VWF A1.
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- 2022
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31. Tyrosine Kinase Inhibitor Sunitinib Delays Platelet-Induced Coagulation: Additive Effects of Aspirin.
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Tullemans BME, Fernández DI, Veninga A, Baaten CCFMJ, Peters LJF, Aarts MJB, Eble JA, Campello E, Spiezia L, Simioni P, van der Vorst EPC, van der Meijden PEJ, Heemskerk JWM, and Kuijpers MJE
- Subjects
- Aspirin metabolism, Aspirin pharmacology, Blood Coagulation physiology, Humans, Platelet Aggregation physiology, Protein Kinase Inhibitors pharmacology, Protein Kinase Inhibitors therapeutic use, Sunitinib therapeutic use, Thrombosis drug therapy, Thrombosis prevention & control, Blood Coagulation drug effects, Platelet Aggregation drug effects, Sunitinib pharmacology
- Abstract
Background: Sunitinib is a multitarget tyrosine kinase inhibitor (TKI) used for cancer treatment. In platelets, sunitinib affects collagen-induced activation under noncoagulating conditions. We investigated (1) the effects of sunitinib on thrombus formation induced by other TK-dependent receptors, and (2) the effects under coagulating conditions. Cardiovascular disease is a comorbidity in cancer patients, resulting in possible aspirin treatment. Sunitinib and aspirin are associated with increased bleeding risk, and therefore we also investigated (3) the synergistic effects of these compounds on thrombus and fibrin formation., Methods: Blood or isolated platelets from healthy volunteers or cancer patients were incubated with sunitinib and/or aspirin or vehicle. Platelet activation was determined by TK phosphorylation, flow cytometry, changes in [Ca
2+ ]i , aggregometry, and whole blood perfusion over multiple surfaces, including collagen with(out) tissue factor (TF) was performed., Results: Sunitinib reduced thrombus formation and phosphatidylserine (PS) exposure under flow on collagen type I and III. Also, sunitinib inhibited glycoprotein VI-induced TK phosphorylation and Ca2+ elevation. Upon TF-triggered coagulation, sunitinib decreased PS exposure and fibrin formation. In blood from cancer patients more pronounced effects of sunitinib were observed in lung and pancreatic as compared to neuroglioblastoma and other cancer types. Compared to sunitinib alone, sunitinib plus aspirin further reduced platelet aggregation, thrombus formation, and PS exposure on collagen under flow with(out) coagulation., Conclusion: Sunitinib suppresses collagen-induced procoagulant activity and delays fibrin formation, which was aggravated by aspirin. Therefore, we urge for awareness of the combined antiplatelet effects of TKIs with aspirin, as this may result in increased risk of bleeding., Competing Interests: J.W.M.H. is a cofounder and shareholder of FlowChamber B.V. The other authors declare no relevant conflicts of interest., (Thieme. All rights reserved.)- Published
- 2022
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32. Ultra-high-throughput Ca 2+ assay in platelets to distinguish ITAM-linked and G-protein-coupled receptor activation.
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Fernández DI, Provenzale I, Cheung HYF, van Groningen J, Tullemans BME, Veninga A, Dunster JL, Honarnejad S, van den Hurk H, Kuijpers MJE, and Heemskerk JWM
- Abstract
Antiplatelet drugs targeting G-protein-coupled receptors (GPCRs), used for the secondary prevention of arterial thrombosis, coincide with an increased bleeding risk. Targeting ITAM-linked receptors, such as the collagen receptor glycoprotein VI (GPVI), is expected to provide a better antithrombotic-hemostatic profile. Here, we developed and characterized an ultra-high-throughput (UHT) method based on intracellular [Ca
2+ ]i increases to differentiate GPVI and GPCR effects on platelets. In 96-, 384-, or 1,536-well formats, Calcium-6-loaded human platelets displayed a slow-prolonged or fast-transient [Ca2+ ]i increase when stimulated with the GPVI agonist collagen-related peptide or with thrombin and other GPCR agonists, respectively. Semi-automated curve fitting revealed five parameters describing the Ca2+ responses. Verification of the UHT assay was done with a robustness compound library and clinically relevant platelet inhibitors. Taken together, these results present proof of principle of distinct receptor-type-dependent Ca2+ signaling curves in platelets, which allow identification of new inhibitors in a UHT way., Competing Interests: J.W.M.H. is a cofounder and shareholder of FlowChamber B.V. The other authors declare no relevant conflicts of interest., (© 2021 The Author(s).)- Published
- 2021
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33. Platelet calcium signaling by G-protein coupled and ITAM-linked receptors regulating anoctamin-6 and procoagulant activity.
- Author
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Fernández DI, Kuijpers MJE, and Heemskerk JWM
- Subjects
- Humans, Anoctamins metabolism, Blood Platelets metabolism, Calcium Signaling immunology, GTP-Binding Proteins metabolism
- Abstract
Most agonists stimulate platelet Ca
2+ rises via G-protein coupled receptors (GPCRs) or ITAM-linked receptors (ILRs). Well studied are the GPCRs stimulated by the soluble agonists thrombin (PAR1, PAR4), ADP (P2Y1 , P2Y12 ), and thromboxane A2 (TP), signaling via phospholipase (PLC)β isoforms. The platelet ILRs glycoprotein VI (GPVI), C-type lectin-like receptor 2 (CLEC2), and FcγRIIa are stimulated by adhesive ligands or antibody complexes and signal via tyrosine protein kinases and PLCγ isoforms. Marked differences exist between the GPCR- and ILR-induced Ca2+ signaling in: (i) dependency of tyrosine phosphorylation; (ii) oscillatory versus continued Ca2+ rises by mobilization from the endoplasmic reticulum; and (iii) smaller or larger role of extracellular Ca2+ entry via STIM1/ORAI1. Co-stimulation of both types of receptors, especially by thrombin (PAR1/4) and collagen (GPVI), leads to a highly enforced Ca2+ rise, involving mitochondrial Ca2+ release, which activates the ion and phospholipid channel, anoctamin-6. This highly Ca2+ -dependent process causes swelling, ballooning, and phosphatidylserine expression, establishing a unique platelet population swinging between vital and necrotic (procoagulant 'zombie' platelets). Additionally, the high Ca2+ status of procoagulant platelets induces a set of additional events: (i) Ca2+ dependent cleavage of signaling proteins and receptors via calpain and ADAM isoforms; (ii) microvesiculation; (iii) enhanced coagulation factor binding; and (iv) fibrin-coat formation involving transglutaminases. Given the additive roles of GPCR and ILR in Ca2+ signal generation, high-throughput screening of biomolecules or small molecules based on Ca2+ flux measurements provides a promising way to find new inhibitors interfering with prolonged high Ca2+ , phosphatidylserine expression, and hence platelet procoagulant activity.- Published
- 2021
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34. HLA association with the susceptibility to anti-synthetase syndrome.
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Remuzgo-Martínez S, Atienza-Mateo B, Ocejo-Vinyals JG, Pulito-Cueto V, Prieto-Peña D, Genre F, Marquez A, Llorca J, Mora Cuesta VM, Fernández DI, Riesco L, Ortego-Centeno N, Gómez NP, Mera A, Martínez-Barrio J, López-Longo FJ, Lera-Gómez L, Moriano C, Díez E, Tomero E, Calvo-Alén J, Romero-Bueno F, Sanchez-Pernaute O, Nuño L, Bonilla G, Grafia I, Prieto-González S, Narvaez J, Trallero-Araguas E, Selva-O'Callaghan A, Gualillo O, Martín J, Cavagna L, Castañeda S, Cifrian JM, Renzoni EA, López-Mejías R, and González-Gay MA
- Subjects
- Alleles, Antibodies, Antinuclear, Autoantibodies, Case-Control Studies, Genetic Predisposition to Disease, HLA Antigens, HLA-DRB1 Chains genetics, Humans, Ligases, Myositis genetics
- Abstract
Objective: To investigate the human leukocyte antigen (HLA) association with anti-synthetase syndrome (ASSD)., Methods: We conducted the largest immunogenetic HLA-DRB1 and HLA-B study to date in a homogeneous cohort of 168 Caucasian patients with ASSD and 486 ethnically matched healthy controls by sequencing-based-typing., Results: A statistically significant increase of HLA-DRB1*03:01 and HLA-B*08:01 alleles in patients with ASSD compared to healthy controls was disclosed (26.2% versus 12.2%, P=1.56E-09, odds ratio-OR [95% confidence interval-CI]=2.54 [1.84-3.50] and 21.4% versus 5.5%, P=18.95E-18, OR [95% CI]=4.73 [3.18-7.05]; respectively). Additionally, HLA-DRB1*07:01 allele was significantly decreased in patients with ASSD compared to controls (9.2% versus 17.5%, P=0.0003, OR [95% CI]=0.48 [0.31-0.72]). Moreover, a statistically significant increase of HLA-DRB1*03:01 allele in anti-Jo-1 positive compared to anti-Jo-1 negative patients with ASSD was observed (31.8% versus 15.5%, P=0.001, OR [95% CI]=2.54 [1.39-4.81]). Similar findings were observed when HLA carrier frequencies were assessed. The HLA-DRB1*03:01 association with anti-Jo-1 was unrelated to smoking history. No HLA differences in patients with ASSD stratified according to the presence/absence of the most representative non-anti-Jo-1 anti-synthetase autoantibodies (anti-PL-12 and anti-PL-7), arthritis, myositis or interstitial lung disease were observed., Conclusions: Our results support the association of the HLA complex with the susceptibility to ASSD., (Copyright © 2020 Société française de rhumatologie. Published by Elsevier Masson SAS. All rights reserved.)
- Published
- 2021
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35. Influence of MUC5B gene on antisynthetase syndrome.
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López-Mejías R, Remuzgo-Martínez S, Genre F, Pulito-Cueto V, Rozas SMF, Llorca J, Fernández DI, Cuesta VMM, Ortego-Centeno N, Gómez NP, Mera-Varela A, Martínez-Barrio J, López-Longo FJ, Mijares V, Lera-Gómez L, Usetti MP, Laporta R, Pérez V, Gafas AP, González MAA, Calvo-Alén J, Romero-Bueno F, Sanchez-Pernaute O, Nuno L, Bonilla G, Balsa A, Hernández-González F, Grafia I, Prieto-González S, Narvaez J, Trallero-Araguas E, Selva-O'Callaghan A, Gualillo O, Castañeda S, Cavagna L, Cifrian JM, and González-Gay MA
- Subjects
- Adult, Female, Follow-Up Studies, Humans, Incidence, Lung Diseases, Interstitial epidemiology, Male, Middle Aged, Myositis complications, Polymorphism, Single Nucleotide, Promoter Regions, Genetic genetics, Lung Diseases, Interstitial genetics, Mucin-5B genetics, Myositis genetics
- Abstract
MUC5B rs35705950 (G/T) is strongly associated with idiopathic pulmonary fibrosis (IPF) and also contributes to the risk of interstitial lung disease (ILD) in rheumatoid arthritis (RA-ILD) and chronic hypersensitivity pneumonitis (CHP). Due to this, we evaluated the implication of MUC5B rs35705950 in antisynthetase syndrome (ASSD), a pathology characterised by a high ILD incidence. 160 patients with ASSD (142 with ILD associated with ASSD [ASSD-ILD+]), 232 with ILD unrelated to ASSD (comprising 161 IPF, 27 RA-ILD and 44 CHP) and 534 healthy controls were genotyped. MUC5B rs35705950 frequency did not significantly differ between ASSD-ILD+ patients and healthy controls nor when ASSD patients were stratified according to the presence/absence of anti Jo-1 antibodies or ILD. No significant differences in MUC5B rs35705950 were also observed in ASSD-ILD+ patients with a usual interstitial pneumonia (UIP) pattern when compared to those with a non-UIP pattern. However, a statistically significant decrease of MUC5B rs35705950 GT, TT and T frequencies in ASSD-ILD+ patients compared to patients with ILD unrelated to ASSD was observed. In summary, our study does not support a role of MUC5B rs35705950 in ASSD. It also indicates that there are genetic differences between ILD associated with and that unrelated to ASSD.
- Published
- 2020
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36. Role of Platelet Glycoprotein VI and Tyrosine Kinase Syk in Thrombus Formation on Collagen-Like Surfaces.
- Author
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Jooss NJ, De Simone I, Provenzale I, Fernández DI, Brouns SLN, Farndale RW, Henskens YMC, Kuijpers MJE, Ten Cate H, van der Meijden PEJ, Cavill R, and Heemskerk JWM
- Subjects
- Blood Platelets drug effects, Blood Platelets physiology, Cells, Cultured, Collagen chemistry, Cyclohexylamines pharmacology, Humans, Peptide Fragments chemistry, Peptide Fragments metabolism, Platelet Aggregation, Protein Kinase Inhibitors pharmacology, Pyrimidines pharmacology, Syk Kinase antagonists & inhibitors, Blood Platelets metabolism, Collagen metabolism, Platelet Membrane Glycoproteins metabolism, Syk Kinase metabolism, Thrombosis metabolism
- Abstract
Platelet interaction with collagens, via von Willebrand factor, is a potent trigger of shear-dependent thrombus formation mediated by subsequent engagement of the signaling collagen receptor glycoprotein (GP)VI, enforced by integrin α
2 β1 . Protein tyrosine kinase Syk is central in the GPVI-induced signaling pathway, leading to elevated cytosolic Ca2+ . We aimed to determine the Syk-mediated thrombogenic activity of several collagen peptides and (fibrillar) type I and III collagens. High-shear perfusion of blood over microspots of these substances resulted in thrombus formation, which was assessed by eight parameters and was indicative of platelet adhesion, activation, aggregation, and contraction, which were affected by the Syk inhibitor PRT-060318. In platelet suspensions, only collagen peptides containing the consensus GPVI-activating sequence (GPO)n and Horm-type collagen evoked Syk-dependent Ca2+ rises. In whole blood under flow, Syk inhibition suppressed platelet activation and aggregation parameters for the collagen peptides with or without a (GPO)n sequence and for all of the collagens. Prediction models based on a regression analysis indicated a mixed role of GPVI in thrombus formation on fibrillar collagens, which was abolished by Syk inhibition. Together, these findings indicate that GPVI-dependent signaling through Syk supports platelet activation in thrombus formation on collagen-like structures regardless of the presence of a (GPO)n sequence.- Published
- 2019
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37. Adiponectin accounts for gender differences in hepatocellular carcinoma incidence.
- Author
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Manieri E, Herrera-Melle L, Mora A, Tomás-Loba A, Leiva-Vega L, Fernández DI, Rodríguez E, Morán L, Hernández-Cosido L, Torres JL, Seoane LM, Cubero FJ, Marcos M, and Sabio G
- Subjects
- AMP-Activated Protein Kinases metabolism, Adipocytes metabolism, Adiponectin genetics, Adipose Tissue metabolism, Animals, Cohort Studies, Female, Gallstones surgery, Humans, Incidence, Liver metabolism, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Mitogen-Activated Protein Kinase 14 metabolism, Obesity metabolism, Sex Factors, Adiponectin blood, Carcinoma, Hepatocellular epidemiology, Gallstones blood, Liver Neoplasms epidemiology
- Abstract
Hepatocellular carcinoma (HCC) is the sixth most common cancer type and the fourth leading cause of cancer-related death. This cancer appears with higher incidence in men and during obesity; however, the specific mechanisms underlying this correlation are unknown. Adipose tissue, a key organ in metabolic syndrome, shows evident gender disparities in the production of adipokines. Levels of the important adipokine adiponectin decrease in men during puberty, as well as in the obese state. Here, we show that this decrease in adiponectin levels is responsible for the increased liver cancer risk in males. We found that testosterone activates the protein JNK in mouse and human adipocytes. JNK-mediated inhibition of adiponectin secretion increases liver cancer cell proliferation, since adiponectin protects against liver cancer development through the activation of AMP-activated protein kinase (AMPK) and p38α. This study provides insight into adipose tissue to liver crosstalk and its gender relation during cancer development, having the potential to guide strategies for new cancer therapeutics., (© 2019 Manieri et al.)
- Published
- 2019
- Full Text
- View/download PDF
38. [Key features in the management of diffuse interstitial pulmonary disease].
- Author
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Fernández DI, Sánchez RP, Moreno AF, and Fernández Fabrellas E
- Subjects
- Humans, Hypertension, Pulmonary diagnosis, Hypertension, Pulmonary etiology, Hypertension, Pulmonary therapy, Lung Diseases, Interstitial complications, Pulmonary Fibrosis diagnosis, Pulmonary Fibrosis therapy, Lung Diseases, Interstitial diagnosis, Lung Diseases, Interstitial therapy
- Abstract
Diffuse interstitial lung disease is a heterogeneous group of diseases in which the common denominator is involvement of the area between the basement membrane of the alveolar epithelium and the capillary endothelium, known as the interstitial space. Diffuse interstitial lung disease poses a tremendous challenge to the clinician due to the diagnostic approach, the complications that can appear in the natural history of these entities, and the scarcity of available therapeutic resources. This brief review discusses key features of the management of diffuse interstitial pulmonary disease, such as prognostic factors, the therapeutic options -including the role of lung transplantation- and the diagnosis and treatment of two complications with crucial impact on the clinical course of the disease: exacerbations and associated pulmonary hypertension., (Copyright © 2009 Sociedad Española de Neumología y Cirugía Torácica. Published by Elsevier Espana. All rights reserved.)
- Published
- 2009
- Full Text
- View/download PDF
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