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24 results on '"Ferguson, Cristin M."'

6. HIF_SupplementaryInformation_Revised – Supplemental material for Role of the Hypoxia-Inducible Factor Pathway in Normal and Osteoarthritic Meniscus and in Mice after Destabilization of the Medial Meniscus

Author

Stone, Austin V., Loeser, Richard F., Callahan, Michael F., McNulty, Margaret A., Long, David L., Raghunatha R. Yammani, Bean, Sara, Kadie Vanderman, Chubinskaya, Susan, and Ferguson, Cristin M.

Subjects
FOS: Clinical medicine, FOS: Biological sciences, 110323 Surgery, 110604 Sports Medicine, FOS: Health sciences, 69999 Biological Sciences not elsewhere classified, 110314 Orthopaedics
Abstract

Supplemental material, HIF_SupplementaryInformation_Revised for Role of the Hypoxia-Inducible Factor Pathway in Normal and Osteoarthritic Meniscus and in Mice after Destabilization of the Medial Meniscus by Austin V. Stone, Richard F. Loeser, Michael F. Callahan, Margaret A. McNulty, David L. Long, Raghunatha R. Yammani, Sara Bean, Kadie Vanderman, Susan Chubinskaya and Cristin M. Ferguson in CARTILAGE

Published
2021
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10. Role of the Hypoxia-Inducible Factor Pathway in Normal and Osteoarthritic Meniscus and in Mice after Destabilization of the Medial Meniscus

Author

Stone, Austin V., primary, Loeser, Richard F., additional, Callahan, Michael F., additional, McNulty, Margaret A., additional, Long, David L., additional, Yammani, Raghunatha R., additional, Bean, Sara, additional, Vanderman, Kadie, additional, Chubinskaya, Susan, additional, and Ferguson, Cristin M., additional

Published
2020
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14. Role of the Hypoxia-Inducible Factor Pathway in Normal and Osteoarthritic Meniscus and in Mice after Destabilization of the Medial Meniscus

Author

Stone, Austin V., Loeser, Richard F., Callahan, Michael F., McNulty, Margaret A., Long, David L., Yammani, Raghunatha R., Bean, Sara, Vanderman, Kadie, Chubinskaya, Susan, and Ferguson, Cristin M.

Abstract

Objective Meniscus injury and the hypoxia-inducible factor (HIF) pathway are independently linked to osteoarthritis pathogenesis, but the role of the meniscus HIF pathway remains unclear. We sought to identify and evaluate HIF pathway response in normal and osteoarthritic meniscus and to examine the effects of Epas1 (HIF-2α) insufficiency in mice on early osteoarthritis development.Methods Normal and osteoarthritic human meniscus specimens were obtained and used for immunohistochemical evaluation and cell culture studies for the HIF pathway. Meniscus cells were treated with pro-inflammatory stimuli, including interleukins (IL)-1β, IL-6, transforming growth factor (TGF)-α, and fibronectin fragments (FnF). Target genes were also evaluated with HIF-1α and HIF-2α (Epas1) overexpression and knockdown. Wild-type (n= 36) and Epas1+/−(n= 30) heterozygous mice underwent destabilization of the medial meniscus (DMM) surgery and were evaluated at 2 and 4 weeks postoperatively for osteoarthritis development using histology.Results HIF-1α and HIF-2α immunostaining and gene expression did not differ between normal and osteoarthritic meniscus. While pro-inflammatory stimulation significantly increased both catabolic and anabolic gene expression in the meniscus, HIF-1α and Epas1 expression levels were not significantly altered. Epas1 overexpression significantly increased Col2a1 expression. Both wild-type and Epas1+/−mice developed osteoarthritis following DMM surgery. There were no significant differences between genotypes at either time point.Conclusion The HIF pathway is likely not responsible for osteoarthritic changes in the human meniscus. Additionally, Epas1 insufficiency does not protect against osteoarthritis development in the mouse at early time points after DMM surgery. The HIF pathway may be more important for protection against catabolic stress.

Published
2021
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22. Surgical Treatment of Chronic Posteromedial Instability Using Capsular Procedures.

Author

Jackson III, J. Benjamin, Ferguson, Cristin M., and Martin, David F.

Subjects
*OPERATIVE surgery, *ANTERIOR cruciate ligament, *POSTERIOR cruciate ligament, *MEDIAL collateral ligament (Knee), *KNEE injuries, *COLLATERAL ligament, *CRUCIATE ligaments, *JOINT hypermobility, *LIGAMENTS
Abstract

The article reports on the surgical treatment of chronic posteromedial instability using capsular procedures. Anteromedial rotatory instability symptoms are often overlooked due to concurrent anterior cruciate ligament and posterior cruciate ligament injuries. Unrecognition and nontreatment of the damage results in residual functional laxity and may lead to chronic and progressive instability of the knee. Two surgical techniques addressing posteromedial instability that requires an understanding of posteromedial corner knee anatomy, careful preoperative planning, and extensive postoperative rehabilitation are discussed.

Published
2006
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23. Tissue Engineering Cartilage with a Composite Electrospun and Hydrogel Scaffold

Author

Wright, Lee David, Biomedical Engineering, Freeman, Joseph W., Rylander, M. Nichole, Lee, Yong Woo, Goldstein, Aaron S., and Ferguson, Cristin M.

Subjects
tissue engineering, technology, industry, and agriculture, hydrogel, scaffold, cartilage, electrospinning
Abstract

Osteoarthritis is the most prevalent musculoskeletal disease in humans, severely reducing the standard of living of millions of people. Osteoarthritis is characterized by degeneration and loss of articular cartilage which leads to pain, and loss of joint motility and function. Individuals suffering from severe osteoarthritis are commonly treated with full knee replacements. The procedure does eliminate the problem of degrading cartilage tissue; however, it does not fully restore function and its lifetime can be limited. To overcome the disadvantages of current treatments, tissue engineering has become a focus of research to regenerate cartilage. Tissue engineering attempts to repair or replace damaged tissue with cells, biomaterials, and/or molecular signals. Biodegradable scaffolds serve as a temporary replacement for the tissue until it has regenerated. Two types of scaffolds that have been used in tissue engineering are electrospun scaffolds and hydrogels. We have proposed and fabricated a scaffold for cartilage tissue engineering that incorporates an electrospun cylinder and a thermosetting hydrogel in order to provide improved properties compared to either individual material. Electrospun cylinders were created by sintering electrospun mats that include salt pores. The addition of salt pores decreased the mechanical properties of the electrospun materials while also improving the capability of cells to infiltrate into the scaffold. The sintering process involved the connecting of one electrospun mat to an adjacent one. Specifically, poly(d,l-lactide) was capable of sintering to an adjacent electrospun mat when exposed to either heat (near the glass transition temperature) or tetrahydrofuran vapor. The sintering process did not deteriorate the structure or function of the electrospun material. Sintering allowed the creation of unique structures of electrospun material that previously could not be produced. A thermosetting hydrogel was added to the scaffold to replicate the function of proteoglycans present in articular cartilage. A composite scaffold of electrospun polymer and hydrogel showed improved mechanical properties and better integration of the scaffold in vivo compared to an electrospun scaffold with no hydrogel. In conclusion, the composite electrospun and hydrogel scaffold could become an excellent tissue engineering scaffold to treat patients suffering from osteoarthritis. Ph. D.

Published
2011

24. Variability in Patellofemoral Alignment Measurements on MRI: Influence of Knee Position.

Author

Marquez-Lara A, Andersen J, Lenchik L, Ferguson CM, and Gupta P

Subjects
Adult, Anatomic Landmarks, Female, Healthy Volunteers, Humans, Image Processing, Computer-Assisted, Male, Range of Motion, Articular, Magnetic Resonance Imaging methods, Patellofemoral Joint anatomy & histology, Patellofemoral Joint diagnostic imaging
Abstract

Objective: The purpose of this study was to determine the variability of distances between four distinct anatomic landmarks used for the evaluation of patellofemoral alignment while implementing controlled changes in patient positioning during MRI., Materials and Methods: Limited MRI was performed of 12 knees in healthy volunteers (10 men, two women; mean age, 28 years) with the knees in four different positions. The four landmarks used were TT (the most anterior point of the osseous tibial tubercle), TG (the nadir of the cartilaginous trochlear groove), PT (the center of the patellar tendon insertion on the tibia), and PCL (the medial border of the posterior cruciate ligament at its insertion along the posterior tibia). TT-TG, PT-TG, TT-PCL, and PT-PCL distances were measured on the MR images. Each distance was measured with the knee at maximum patient comfort (rest), full extension, 15° of flexion, and 30° of flexion. Linear mixed models with random intercepts were implemented to determine variability between measurements and knee position., Results: In general, measurements based on anatomic landmark and knee position varied greatly. The greatest variability in different knee positions was seen in mean TT-TG and PT-TG distances ± SD (TT-TG: rest, 18.1 ± 7.9 mm; full extension, 17.3 ± 5.3 mm; 15° of flexion, 11.4 ± 5.7 mm; 30° of flexion, 11.7 ± 6.0 mm; intraclass correlation coefficient [ICC] = 0.539; PT-TG: rest, 18.1 ± 6.3 mm; full extension, 17.9 ± 4.7 mm; 15° of flexion, 13.2 ± 5.2 mm; 30° of flexion, 11.8 ± 4.3 mm; ICC = 0.633). In contrast, knee position did not significantly affect distances for TT-PCL and PT-PCL (TT-PCL: rest, 23.5 ± 6.8 mm; full extension, 20.5 ± 5.5 mm; 15° of flexion, 22.8 ± 6.2 mm; 30° of flexion, 22.8 ± 6.7 mm; ICC = 0.484; PTPCL: rest, 23.4 ± 5.3 mm; full extension, 21.5 ± 4.5 mm; 15° of flexion, 22.3 ± 4.3 mm; 30° of flexion, 23.1 ± 4.8 mm; ICC = 0.509)., Conclusion: On MRI, TT-PCL and PT-PCL measurements have significantly less variability compared with TT-TG and PT-TG measurements, regardless of knee position. Although further studies are warranted, the use of TT-PCL and PT-PCL measurements may offer more reliable assessment of tibial tubercle lateralization and patellofemoral alignment.

Published
2017
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