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In recent years, Brazil, as the world’s fourth-largest democracy, witnessed the dominance of polarized and symbolically charged electoral campaigns on social media, culminating in the election of a populist political figure in 2018 and his subsequent defeat in 2022. Extensive research has indicated that political campaigns often sidelined substantive policy proposals in favor of negative and divisive issues. However, a critical gap remains in the absence of temporal investigations contrasting the prevalence of negativity and acclaim campaigns on social media platforms during elections. This study addresses this gap by examining associations between political issues and negative and acclaim campaigns across two Brazilian electoral campaigns. Drawing upon a sample of messages posted on Twitter (n = 1,191) during the presidential elections of 2018 and 2022, our study reveals associations between substantive political issues, such as education and health, and acclaim campaign strategies, while the divisive issues of Covid-19 and corruption are associated with negative campaign strategies. Moreover, the results suggest that gender policy is related to both acclaim and negative messages since it is a polarizing issue in Brazilian politics. Our study also shows an increased negativity trend, with the 2022 presidential election campaign more likely to be negative than in 2018. By conducting a temporal analysis of Brazil’s political context, our study sheds light on the evolving dynamics of political communication in the age of social media, contributing substantially to the literature on negativity in political campaigns.

Economic, social, and health crises have shaken and polarized contemporary politics. An element fueling this polarization is the dissemination of divisive topics on social media platforms. While these polarizing social media tendencies are increasingly studied, research exploring digital political communication in South America remains scarce. This study aims to analyze the electoral campaigns in Brazil, Colombia, Chile, and Peru to define the features that trigger polarized emotional reactions on Facebook. The corpus comprises a sample of 2,930 posts published by candidates and political parties during the first round of the presidential elections held in these countries between 2021 and 2022. We hypothesize that users are more likely to react in a polarized way to content focused on divisive issues. In addition, we examine how these patterns differ across countries and the influence of the level of political polarization. Finally, the role played by party-level characteristics in the emotional reactions of users is also analyzed. By means of quantitative content analysis, these questions are addressed using multilevel negative binomial regressions to identify what predicts Love and Angry reactions. The bandwagon effect seems to work positively on users’ moods since the most popular political actors receive significantly more Love reactions, irrespective of the post’s subject. In more polarized countries, there is a tendency to react more negatively to certain divisive issues, generating greater visibility of these issues on social networks and thus promoting more polarization. These findings expand knowledge about the dynamics of digital political communication in the Global South.

Abstract In 2020, the COVID-19 pandemic followed a two-wave pattern in most countries. Hospital admission for COVID-19 in one wave or another could have affected mortality, especially among the older persons. The objective of this study was to evaluate whether the admission of older patients during the different waves, before SARS-CoV-2 vaccination was available, was associated with a different mortality. We compared the mortality rates of patients hospitalized during 2020 before (first wave) and after (second wave) July 7, 2020, included in the SEMI-COVID-19 Registry, a large, multicenter, retrospective cohort of patients admitted to 126 Spanish hospitals for COVID-19. A multivariate logistic regression analysis was performed to control for changes in either the patient or disease profile. As of December 26, 2022, 22,494 patients had been included (17,784 from the first wave and 4710 from the second one). Overall mortality was 20.4% in the first wave and 17.2% in the second wave (risk difference (RD) − 3.2%; 95% confidence interval (95% CI) − 4.4 to − 2.0). Only patients aged 70 and older (10,973 patients: 8571 in the first wave and 2386 in the second wave) had a significant reduction in mortality (RD − 7.6%; 95% CI − 9.7 to − 5.5) (unadjusted relative risk reduction: 21.6%). After adjusting for age, comorbidities, variables related to the severity of the disease, and treatment received, admission during the second wave remained a protective factor. In Spain, patients aged 70 years and older admitted during the second wave of the COVID-19 pandemic had a significantly lower risk of mortality, except in severely dependent persons in need of corticosteroid treatment. This effect is independent of patient characteristics, disease severity, or treatment received. This suggests a protective effect of a better standard of care, greater clinical expertise, or a lesser degree of healthcare system overload.

Abstract Background/Objectives Several studies have shown a relationship between vitamin D and migraine, including the association between decreased serum 25‐hydroxyvitamin D in patients with migraine and the positive effects of vitamin D supplementations in the therapy of this disease. Two single‐nucleotide variants (SNVs) vitamin D receptor (VDR) gene, VDR rs2228570, and VDR rs731236 have shown an association with migraine risk in a previous case–control association study, while an exome sequencing study identified a rare variant in GC vitamin D binding protein gene. This study aims to look for the association between several common variants in these two genes and the risk for migraine. Methods We genotyped 290 patients diagnosed with migraine and 300 age‐matched controls using specific TaqMan assays for VDR rs2228570, VDR rs731236, VDR rs7975232, VDR rs739837, VDR rs78783628, GC rs7041, and GC rs4588 SNVs. Results We did not find an association between these SNVs and the risk for migraine. None of these SNVs were related to the positivity of a family history of migraine or with the presence of aura. The VDR rs731236A allele showed a significant association with the triggering of migraine attacks by ethanol (Pc = 0.007). Conclusions In summary, the results of the current study suggest a lack of association between common SNVs in the VDR and GC gene and the risk of developing migraine. The possible relationship between VDR rs731236 and the triggering of migraine episodes with ethanol deserves future studies.

The high genetic diversity of the tomato and its high micronutrient content make this fruit very interesting from an economic and nutritional point of view. The genetic erosion suffered by this crop, due to breeding objectives based on yield and marketing, makes it necessary to return to the origins in search of the nutritional and organoleptic quality lost in traditional varieties. In this study, the agronomic, physical, organoleptic, and nutritional characteristics of eighteen F1 hybrids, obtained by crossing fourteen traditional varieties, previously selected for their quality, were studied in order to select genotypes of superior quality that could be candidates for new varieties. All the parameters studied were strongly influenced by genotype, with a wide range between varieties. Most of the experimental hybrids showed higher quality scores than the commercial hybrids used as controls, due to the extensive selection process carried out on the parents in previous work. Principal component analysis revealed the characteristics of each hybrid that distinguished it from the others. Some hybrids (H1, H2, and H4) stood out for their high concentration of active compounds, others (H14, H13, H8, H15, H7, and H9) for their agronomic performance and high β-carotene content, and H3 was the only one to contain chlorophyll in its ripe fruits. Finally, the evaluation index allowed the selection of five hybrids with interesting characteristics, combining good yield performance and high quality. The results of this work have allowed for the selection of a group of hybrids with high organoleptic and nutritional quality which will be used as parents in a breeding programme, in which their characteristics will be fixed and their resilience will be increased through the introduction of virus resistance.

Abstract Background Conjugate vaccines, such as the 7-valent conjugate vaccine (PCV7), alter serotype nasopharyngeal carriage, potentially increasing cases of otitis media by non-vaccine serotypes. Methods All paediatric middle ear fluid (MEF) isolates received in the Spanish Reference Laboratory for Pneumococci through a passive, laboratory-based surveillance system from January 1997 to June 2009 were analysed. Data from 1997 to 2000 were pooled as pre-vaccination period. Trends over time were explored by linear regression analysis. Results A total of 2,077 isolates were analysed: 855 belonging to PCV7 serotypes, 466 to serotype 19A, 215 to serotype 3, 89 to serotype 6A and 452 to other serotypes (< 40 isolates each). Over time, there has been a decreasing trend for PCV7 serotypes (R2 = 0.944; p < 0.001, with significant decreasing trends for serotypes 19F, 14, 23F and 9V), and increasing trends for serotype 19A (R2 = 0.901; p < 0.001), serotype 3 (R2 = 0.463; p = 0.030) and other non-PCV7 serotypes (R2 = 0.877; p < 0.001), but not for serotype 6A (R2 = 0.311; p = 0.094). Considering all isolates, amoxicillin non-susceptibility showed an increasing trend (R2 = 0.528; p = 0.017). Regarding serotype 19A, increasing trends in non-susceptibility to penicillin (R2 = 0.726; p = 0.001), amoxicillin (R2 = 0.804; p < 0.001), cefotaxime (R2 = 0.546; p = 0.005) and erythromycin (R2 = 0.546; p = 0.009) were found, with amoxicillin non-susceptibility firstly detected in 2003 (7.4%) and increasing up to 38.0% in 2009. In PCV7 serotypes (which prevalence decreased from 70.7% during 1997-2000 to 10.6% in 2009) amoxicillin non-susceptibility rates showed an increasing trend (R2 = 0.702; p = 0.002). However, overall, amoxicillin non-susceptibility (≈25% in 2008-9) could be mainly attributed to serotype 19A (> 35% isolates) since PCV7 strains represented < 11% of total clinical isolates. Conclusions In contrast to reports on invasive pneumococcal strains, in MEF isolates the reduction in the prevalence of PCV7 serotypes was not associated with decreases in penicillin/erythromycin non-susceptibility. The high prevalence of serotype 19A among paediatric MEF isolates and the amoxicillin non-susceptibility found in this serotype are worrisome since amoxicillin is the most common antibiotic used in the treatment of acute otitis media. These data suggest that non-PCV7 serotypes (mainly serotype 19A followed by serotypes 3 and 6A) are important etiological agents of acute otitis media and support the added value of the broader coverage of the new 13-valent conjugate vaccine.

The present work investigates the nutritional and bioactive composition, as well as the organoleptic and sensory properties, of S. oleraceus and S. tenerrimus, two wild plant species traditionally used in the gastronomy of the Mediterranean area. Additionally, the effect of cultivation on leaf composition was assessed to explore their potential for large-scale production and commercialization from the point of view of possible losses or gains in quality. Both species were characterized as a good source of bioactive compounds, such as vitamins, pro-vitamins and carotenoids, with health-promoting and antioxidant properties that are highly appreciated. The sensory profile revealed a good general acceptance of S. oleraceus and S. tenerrimus, indicating that they could be included in the diet. Although the cultivation of S. oleraceus resulted in a decrease in the concentration of phenolic compounds when compared to wild-harvested plants, the opposite occurred for vitamin C. In S. tenerrimus, cultivation also increased the concentration of other compounds with important nutritional and healthy properties, such as sugars, organic acids and β-carotene. The results of the composition, organoleptic and sensory properties of S. oleraceus and S. tenerrimus support the idea of their potential to be used as edible leafy vegetables and as promising assets for functional foods.

A dispersive magnetic solid-phase extraction (DMPSE) procedure has been developed to preconcentrate four synthetic phosphodiesterase type 5 (PDE-5) inhibitors (sildenafil, desmethylsildenafil, vardenafil and tadalafil) from different samples (human hair, energy drinks, lubricating gels and dietary supplements). The target compounds were isolated from the solid samples by solid-liquid extraction in basic aqueous medium and the extract was submitted to DMSPE using multiwalled carbon nanotubes/Fe3O4@polypyrrole (MWCNTs/Fe3O4@PPy). For this, 20 mg of the magnetic nanocomposite was added to 30 mL of sample solution/extract and vortexed for 10 min before proceeding to the desorption step using 1 mL of methanol. Analysis was carried out by liquid chromatography with tandem mass spectrometry using electrospray ionization and a triple quadrupole mass analyzer (LC-ESI-QqQ-MS/MS). Matrix-matched calibration was applied for quantification. Limits of detection ranged between 0.01 and 0.22 ng mL−1 for energy drinks and between 0.25 and 7.5 ng g−1 for hair, lubricating gels, and dietary supplements. Relative standard deviations in the 4.3–12.2% (n = 10) were obtained for repeatability studies. The trueness of the proposed method was studied by fortifying the samples at two concentration levels and recoveries in the 85–112% range were obtained. None of the PDE-5 inhibitors studied were detected in real samples.