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Hydrazine induced toxicity causes serious harm to the health of humans. The detection of N2H4 in vitro and in vivo has attracted a great deal of attention, especially in the context of fluorescent probes. Although some fluorescent N2H4 probes have been reported, only a few operate in purely aqueous media and, as a result, require the use of organic cosolvents which hinders their use in analysis of real samples. In addition, most of the current N2H4 probes are either "off-on" or "on-off" types, in which it is difficult to eliminate interference from background fluorescence commonly occurring in in vitro and in vivo systems. Furthermore, some probes are unable to differentiate hydrazine from other organic amines. To address the above problems, we developed a novel oligo(ethylene glycol)-functionalized fluorescent probe for the detection of N2H4. The probe, which has a donor-π-acceptor (D-π-A)-type structure, is water-soluble, and it can be utilized to selectively detect N2H4 in both colorimetric and ratiometric mode. Furthermore, the probe is able to differentiate hydrazine from other organic amines and can be used to detect hydrazine vapor and for imaging A549 cells and zebrafish.

Through many years of operation, the power grid regulation system has accumulated abundant operation data. It includes measurement data, alarm data, failure and operation event data, power data and planned prediction data. The data of power grid business system exists independently in EMS, OMS, statistical analysis platform, TMR and other different systems. The lack of cross-professional, cross-unit and cross-business data sharing mechanism which forms the information island. At present, through the construction of model data cloud platform, the control center extracts and collects all kinds of data from EMS, OMS, statistical analysis platform and other business systems. However, the data exchange capacity is still inadequate. Therefore, it is urgent to build dispatching data exchange platform. Through the model data cloud platform, the regulator center provides unified service of data receiving/extracting, transformation and publishing which "configurable, monitored and controllable" to form a data exchange hub for the internal and external business systems of the control center. In addition, accessing statistical analysis system to protect information ledger, protection model and operation data. The data of the fault recording network system forms the data exchange hub of the control center. The logical business layer realizes model management, fault and action analysis, operation monitoring, intelligent diagnosis and other application scenarios. At the same time, to meet the needs for friendly data exchange with national research vertically and meet the needs for friendly data exchange with all departments of the provincial company horizontally.

Stochastic dynamics of gene switching and energy dissipation for gene expression are largely unknown, mainly due to the complexity of non-equilibrium mechanisms. Here, based on an important double-deck loop model, the stochastic mechanisms of gene switching and energy dissipation are studied. First, the probability distributions of steady states are calculated theoretically. It is found that the signal can strengthen the choice of gene switching between the “off” and “on” states. Our analysis of energy consumption illustrates that, compared with the synthesis and degradation of proteins, the process of gene switching costs little energy. Our theoretical analysis reveals some interesting insights into the determination of cell state and energy dissipation for gene expression.

Based on the model describing two coupled synthetic clock cells, the synchronization dynamics under stochastic noise are explored. As extrinsic noise from signal is the predominant form of noise for all gene promoters, we investigate the effects of extrinsic noise original from signal molecule by evaluating the order parameters. It is found that strong noise is beneficial for the synchronization of loose-coupling system, while it destroys the synchronization of tight-coupling system. The underlying mechanisms of these two opposite effects are clarified numerically and theoretically. Our research illustrates that (i) when the coupling strength is small, the noise mainly adjusts the period difference of two cells and the system becomes regular. Theoretical study reveals that the mean effect of noise is like to be influx while signal flow is efflux under such a situation. (ii) With the increment of coupling strength, the cells have the same frequency. It is obvious that the noise mainly changes the phase difference between the two cells and destroys the synchronization of the system. (iii) We also demonstrate that, under certain moderate noise intensities, the noise can induce the synchronization order to be the worst. This nonlinear behavior only can be observed in a very narrow region of coupling strength.

To find a more perfect method of treating developmental dislocation of the hip(DDH).From March 1994 to December 2002, on the basis of the ordinary operative method, Pemberton method was improved for 48 cases of DDH 49 articulatio coxae which had superficial and small acetabula but big heads of femur. Osteotomy points were moved higher to enlarge area of bone flap turned over. A piece of full thickness ilium including periosteum was removed as a free bony graft. Capsula articularis growing thicker was made into 2 layers, of which the inner layer was used to tighten articularis and the outer layer was used to stabilize bone flap.All patients were followed up for 18 months to 5 years, the function of articulatio coxae and degree of bony union, inclusion area to head of femur and ischemic necrosis of femoral head were observed through X-ray. According to Zhou Yongde's criteria for the curative effect of congenital dislocation of the hip, there were 18 cases of 26-30 scores(3 hips), 27 cases of 21-25 scores, 2 cases of 16-20 scores(3 hips), and 1 case under 15 scores, and the total superior rate was 93.8%.Improved Pemberton method can enlarge inclusion area to head of femur, avoid decreasing acetabular volume, and stabilize articulatio well, which made operative indication bigger than original Pemberton method.