14 results on '"Feng-Guo, Xu"'
Search Results
2. Metabolomics Reveals the Efficacy of Caspase Inhibition for Saikosaponin D-Induced Hepatotoxicity
- Author
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Qian-qian Zhang, Wan-qiu Huang, Yi-qiao Gao, Zhao-di Han, Wei Zhang, Zun-jian Zhang, and Feng-guo Xu
- Subjects
saikosaponin d ,hepatotoxicity ,caspase inhibition ,metabolomics ,bile acids ,inflammation ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Saikosaponin d (SSd) is a major hepatoprotective component of saikosaponins derived from Radix Bupleuri, which was also linked to hepatotoxicity. Previous studies have demonstrated that caspases play a key role in SSd-induced liver cell death. Our in vitro and in vivo studies also showed that treatment with caspase inhibitor z-VAD-fmk could significantly reduce the L02 hepatocyte cells death and lessen the degree of liver damage in mice caused by SSd. In order to further reveal the underlying mechanisms of caspase inhibition in SSd-induced hepatotoxicity, mass spectrometry based untargeted metabolomics was conducted. Significant alterations in metabolic profiling were observed in SSd-treated group, which could be restored by caspase inhibition. Bile acids and phospholipids were screened out to be most significant by spearman correlation analysis, heatmap analysis and S-Plot analysis. These findings were further confirmed by absolute quantitation of bile acids via targeted metabolomics approach. Furthermore, cytokine profiles were analyzed to identify potential associations between inflammation and metabolites. The study could provide deeper insight into the hepatotoxicity of SSd and the efficacy of caspase inhibition.
- Published
- 2018
- Full Text
- View/download PDF
3. Systems Pharmacology Based Strategy for Q-Markers Discovery of HuangQin Decoction to Attenuate Intestinal Damage
- Author
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Xiao-min Dai, Dong-ni Cui, Jing Wang, Wei Zhang, Zun-jian Zhang, and Feng-guo Xu
- Subjects
Q-marker ,systems pharmacology ,traditional Chinese medicine ,HuangQin decoction ,intestinal damage ,Therapeutics. Pharmacology ,RM1-950 - Abstract
The quality control research of traditional Chinese medicine (TCM) is lagged far behind the space of progress in modernization and globalization. Thus the concept of quality marker (Q-marker) was proposed recently to guide the quality investigations of TCM. However, how to discover and validate the Q-marker is still a challenge. In this paper, a system pharmacology based strategy was proposed to discover Q-marker of HuangQin decoction (HQD) to attenuate Intestinal Damage. Using this strategy, nine measurable compounds including paeoniflorin, baicalin, scutellarein, liquiritigenin, norwogonin, baicalein, glycyrrhizic acid, wogonin, and oroxylin A were screened out as potential markers. Standard references of these nine compounds were pooled together as components combination according to their corresponding concentration in HQD. The bioactive equivalence between components combination and HQD was validated using wound healing test and inflammatory factor determination experiment. The comprehensive results indicated that components combination is almost bioactive equivalent to HQD and could serve as the Q-markers. In conclusion, our study put forward a promising strategy for Q-markers discovery.
- Published
- 2018
- Full Text
- View/download PDF
4. Quantitative Evaluation of the Compatibility Effects of Huangqin Decoction on the Treatment of Irinotecan-Induced Gastrointestinal Toxicity Using Untargeted Metabolomics
- Author
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Zun-Jian Zhang, Feng-Guo Xu, Dong-Ni Cui, Xu Wang, Jia-Qing Chen, Bo Lv, Pei Zhang, and Wei Zhang
- Subjects
Huangqin decoction ,delayed diarrhea ,metabolomics ,metabolites deregulation score ,relative area under the curve ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Huangqin decoction (HQD), a traditional Chinese medicine (TCM), has been widely used to treat gastrointestinal syndrome in China for thousands of years. Chemotherapy drug irinotecan (CPT-11) is used clinically to treat various kinds of cancers but limited by its side effects, especially delayed diarrhea. Nowadays, HQD has been proved to be effective in attenuating the intestinal toxicity induced by CPT-11. HQD consists of four medicinal herbs including Scutellaria baicalensis Georgi, Glycyrrhiza uralensis Fisch, Paeonia lactiflora Pall, and Ziziphus jujuba Mill. Due to its complexity, the role of each herb and the multi-herb synergistic effects of the formula are poorly understood. In order to quantitatively assess the compatibility effects of HQD, mass spectrometry-based untargeted metabolomics studies were performed. The serum metabolic profiles of rats administered with HQD, single S. baicalensis decoction, S. baicalensis-free decoction and baicalin/baicalein combination were compared. A time-dependent trajectory upon principal component analysis was firstly used to visualize the overall differences. Then metabolites deregulation score and relative area under the curve were calculated and used as parameters to quantitatively evaluate the compatibility effects of HQD from the aspect of global metabolic profile and the specifically altered metabolites, respectively. The collective results indicated that S. baicalensis played a crucial role in the therapeutic effect of HQD on irinotecan-induced diarrhea. Both HQD and SS decoction regulated glycine, serine and threonine pathway. This study demonstrated that metabolomics was a promising tool to elucidate the compatibility effects of TCM or combinatorial drugs.
- Published
- 2017
- Full Text
- View/download PDF
5. Quantification of phospholipid fatty acids by chemical isotope labeling coupled with atmospheric pressure gas chromatography quadrupole- time-of-flight mass spectrometry (APGC/Q-TOF MS)
- Author
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Ruibing Feng, Feng-Guo Xu, Huanxing Su, Yuanjia Hu, Fangbo Xia, Chengwei He, and Jian-Bo Wan
- Subjects
Phospholipid ,Mice, Transgenic ,02 engineering and technology ,Mass spectrometry ,01 natural sciences ,Biochemistry ,Gas Chromatography-Mass Spectrometry ,Analytical Chemistry ,chemistry.chemical_compound ,Animals ,Environmental Chemistry ,Phospholipids ,Spectroscopy ,Brain Chemistry ,chemistry.chemical_classification ,Detection limit ,Chromatography ,Molecular Structure ,Fatty Acids ,010401 analytical chemistry ,Fatty acid ,Cadherins ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Atmospheric Pressure ,Liver ,chemistry ,Linear range ,Isotope Labeling ,Yield (chemistry) ,Gas chromatography ,Time-of-flight mass spectrometry ,0210 nano-technology - Abstract
Phospholipid fatty acids play the crucial role in biophysical properties and the function of cellular membranes. In the present study, an accurate and sensitive method was developed to quantify phospholipid fatty acids in biological samples by using chemical isotope labeling coupled with atmospheric pressure gas chromatography quadrupole-time-of-flight mass spectrometry (APGC/Q-TOF MS). APGC, a soft ionization source, was operated under proton-transfer condition by introducing methanol into the ionization source as a modifier, which provided high quantifiable molecular ion peaks to substantially enhance the sensitivity. Fatty acid standards were methylated with methanol-d4 to yield FAMEs-d3 that were used as one-to-one internal standards to ensure accurate quantification. Thirty fatty acids in phospholipids were accurately quantified in wide linear range with limit of quantification ranging from 84.6 to 113.2 pg/mL. The newly developed method was successfully applied to quantify phospholipid fatty acids in brain and liver tissues from both fat-1 and WT mice. This method might be expanded to quantify free fatty acids or other conjugated fatty acids in biological samples or other matrices.
- Published
- 2019
6. Effect of Phyllanthus amarus Extract on 5-Fluorouracil-Induced Perturbations in Ribonucleotide and Deoxyribonucleotide Pools in HepG2 Cell Line
- Author
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Jian-Ru Guo, Qian-Qian Chen, Christopher Wai-Kei Lam, Cai-Yun Wang, Feng-Guo Xu, Bu-Ming Liu, and Wei Zhang
- Subjects
Phyllanthus amarus extract ,5-fluorouracil ,ribonucleotides ,deoxyribonucleotides ,Organic chemistry ,QD241-441 - Abstract
The aim of this study was to investigate the antitumor activities of Phyllanthus amarus (PHA) and its potential of herb–drug interactions with 5-Fluorouracil (5-FU). Cell viability, ribonucleotides (RNs) and deoxyribonucleotides (dRNs) levels, cell cycle distribution, and expression of thymidylate synthase (TS) and ribonucleotide reductase (RR) proteins were measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, high performance liquid chromatography tandem mass spectrometry (HPLC/MS/MS) method, flow cytometry and Western blot analysis, respectively. Our standardized PHA extract showed toxicity to HepG2 cells at high concentrations after 72 h exposure and induced G2/M cell cycle arrest. Combined use of 5-FU with PHA resulted in significant decreases in ATP, CTP, GTP, UTP and dTTP levels, while AMP, CMP, GMP and dUMP levels increased significantly compared with use of 5-FU alone. Further, PHA could increase the role of cell cycle arrest at S phase induced by 5-FU. Although PHA alone had no direct impact on TS and RR, PHA could change the levels of RNs and dRNs when combined with 5-FU. This may be due to cell cycle arrest or regulation of key enzyme steps in intracellular RNs and dRNs metabolism.
- Published
- 2016
- Full Text
- View/download PDF
7. Quantitative profiling of eicosanoids derived from n-6 and n-3 polyunsaturated fatty acids by twin derivatization strategy combined with LC-MS/MS in patients with type 2 diabetes mellitus
- Author
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Jian-Bo Wan, Fangbo Xia, Meng Ren, Feng-Guo Xu, and Chengwei He
- Subjects
Electrospray ionization ,Endogeny ,02 engineering and technology ,Mass spectrometry ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,chemistry.chemical_compound ,Tandem Mass Spectrometry ,Fatty Acids, Omega-6 ,Fatty Acids, Omega-3 ,Environmental Chemistry ,Humans ,Derivatization ,Spectroscopy ,chemistry.chemical_classification ,Chromatography ,Molecular Structure ,Eicosanoid metabolism ,010401 analytical chemistry ,Selected reaction monitoring ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Eicosanoid ,chemistry ,Diabetes Mellitus, Type 2 ,cardiovascular system ,Eicosanoids ,lipids (amino acids, peptides, and proteins) ,0210 nano-technology ,Polyunsaturated fatty acid ,Chromatography, Liquid - Abstract
Eicosanoids derived from n-6 and n-3 polyunsaturated fatty acids (PUFAs), serving as important signaling molecules, are implicated in many physiological and pathological processes, including Type 2 diabetes mellitus (T2DM). However, the quantification of endogenous eicosanoids is challenged by high structural similarity, low abundance in biological sample and poor electrospray ionization efficiency. In the current study, a sensitive and accurate liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed to quantify 65 eicosanoids derived from n-6 and n-3 PUFAs in plasma samples using twin derivatization strategy with a pair of reagents, 5-(dimethylamino) naphthalene-1-sulfonyl piperazine (Dns-PP) and (diethylamino) naphthalene-1-sulfonyl piperazine (Dens-PP). Dns-PP-derivatized plasma sample was mixed with the equal volume of Dens-PP-derivatized eicosanoid internal standards for LC-MS/MS analysis in multiple reaction monitoring (MRM) mode. After Dns-PP derivatization, the ionization efficiency and separation performance were substantially improved, resulting in the enhanced sensitivity by 446- to 1009-folds compared to intact eicosanoids. The quantitative accuracy determined by twin derivatization method was found to be comparable with stable isotope labeled internal standards (SIL-IS) method. The newly proposed method was successfully employed to quantify the target eicosanoids in plasma samples from healthy controls and the patients with T2DM. N-6 PUFA-derived eicosanoids, PGF2α, PGD2, PGE2, PGA2, PGB2, 20-HETE and LTC4, significantly increased in plasma sample of T2DM patients. Oppositely, n-3 PUFA-derived eicosanoids, RvE1, 12(S)-HEPE and RvD1, remarkably decreased. Spearman's correlation analysis indicated the strong correlations between these highlighted eicosanoids and clinical parameters of T2DM. Collectively, the sensitive and reliable eicosanoid quantification method may facilitate to elucidate the characteristics of eicosanoid metabolism and understand the role of eicosanoids in the pathogenesis of T2DM and other diseases.
- Published
- 2020
8. Liver metabolomics study reveals protective function of Phyllanthus urinaria against CCl 4 -induced liver injury
- Author
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Liu Buming, Zunjian Zhang, Feng-Guo Xu, Jiaqing Chen, Qian-Qian Zhang, Wei Zhang, Hongcong Qiu, and Qing Guo
- Subjects
0301 basic medicine ,Liver injury ,biology ,business.industry ,CCL4 ,General Medicine ,Traditional Chinese medicine ,Pharmacology ,biology.organism_classification ,Taurocholic acid ,medicine.disease ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,Metabolomics ,Complementary and alternative medicine ,Hepatoprotection ,chemistry ,Biochemistry ,Drug Discovery ,Carbon tetrachloride ,Medicine ,business ,Phyllanthus urinaria - Abstract
Phyllanthus Urinaria L. (PUL) is a traditional Chinese medicine used to treat hepatic and renal disorders. However, the mechanism of its hepatoprotective action is not fully understood. In the present study, blood biochemical indexes and liver histopathological changes were used to estimate the extent of hepatic injury. GC/MS and LC/MS-based untargeted metabolomics were used in combination to characterize the potential biomarkers associated with the protective activity of PUL against CCl4-induced liver injury in rats. PUL treatment could reverse the increase in ALT, AST and ALP induced by CCl4 and attenuate the pathological changes in rat liver. Significant changes in liver metabolic profiling were observed in PUL-treated group compared with liver injury model group. Seventeen biomarkers related to the hepatoprotective effects of PUL against CCl4-induced liver injury were screened out using nonparametric test and Pearson's correlation analysis (OPLS-DA). The results suggested that the potential hepatoprotective effects of PUL in attenuating CCl4-induced hepatotoxicity could be partially attributed to regulating L-carnitine, taurocholic acid, and amino acids metabolism, which may become promising targets for treatment of liver toxicity. In conclusion, this study provides new insights into the mechanism of the hepatoprotection of Phyllanthus Urinaria.
- Published
- 2017
9. Discrimination of Citrus reticulata Blanco and Citrus reticulata ‘Chachi’ by gas chromatograph-mass spectrometry based metabolomics approach
- Author
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Chang-Lin Zhou, E-Hu Liu, Feng-Guo Xu, Li-Li Dou, Guodong Zheng, Long Guo, Ping Li, and Li Duan
- Subjects
Citrus ,Chromatography ,010401 analytical chemistry ,Dietary supplement ,04 agricultural and veterinary sciences ,General Medicine ,Biology ,Mass spectrometry ,040401 food science ,01 natural sciences ,Gas Chromatography-Mass Spectrometry ,0104 chemical sciences ,Analytical Chemistry ,0404 agricultural biotechnology ,Metabolomics ,Chemical marker ,Oils, Volatile ,Gas chromatography ,Gas chromatography–mass spectrometry ,Food Science - Abstract
Citri Reticulatae Pericarpium, mainly including the pericarp of Citrus reticulata Blanco and the pericarp of Citrus reticulata 'Chachi', has been consumed daily as food and dietary supplement for centuries. In this study, GC-MS based metabolomics was employed to compare comprehensively the volatile constituents in Citrus reticulata Blanco and Citrus reticulata 'Chachi'. Principal component analysis and orthogonal partial least squares discrimination analysis indicated that samples could be distinguished effectively from one another. Fifteen metabolites were finally identified for use as chemical markers in discrimination of Citri Reticulatae Pericarpium samples. The antimicrobial activity against Gram-negative and Gram-positive bacteria of the volatile oil from Citrus reticulata Blanco and Citrus reticulata 'Chachi' was investigated preliminarily.
- Published
- 2016
10. Liver metabolomics study reveals protective function of Phyllanthus urinaria against CCl
- Author
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Qing, Guo, Qian-Qian, Zhang, Jia-Qing, Chen, Wei, Zhang, Hong-Cong, Qiu, Zun-Jian, Zhang, Bu-Ming, Liu, and Feng-Guo, Xu
- Subjects
Male ,Taurocholic Acid ,Gas Chromatography-Mass Spectrometry ,Rats ,Rats, Sprague-Dawley ,Phyllanthus ,Liver ,Animals ,Humans ,Metabolomics ,Amino Acids ,Chemical and Drug Induced Liver Injury ,Carbon Tetrachloride ,Drugs, Chinese Herbal - Abstract
Phyllanthus Urinaria L. (PUL) is a traditional Chinese medicine used to treat hepatic and renal disorders. However, the mechanism of its hepatoprotective action is not fully understood. In the present study, blood biochemical indexes and liver histopathological changes were used to estimate the extent of hepatic injury. GC/MS and LC/MS-based untargeted metabolomics were used in combination to characterize the potential biomarkers associated with the protective activity of PUL against CCl
- Published
- 2016
11. Quantitation of camellianin A in HepG2 cells using a high performance liquid chromatography-electrospray ionization tandem mass spectrometric method
- Author
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Cai-Yun Wang, Feng-Guo Xu, Wei Zhang, Hai-Ling Xi, and Qian-Qian Chen
- Subjects
0301 basic medicine ,Flavonoids ,Spectrometry, Mass, Electrospray Ionization ,Chromatography ,Tandem ,Molecular Structure ,Formic acid ,Electrospray ionization ,Extraction (chemistry) ,Selected reaction monitoring ,Analytical chemistry ,General Medicine ,Hep G2 Cells ,Mass spectrometry ,High-performance liquid chromatography ,Triple quadrupole mass spectrometer ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,Complementary and alternative medicine ,chemistry ,Tandem Mass Spectrometry ,Drug Discovery ,Humans ,Chromatography, High Pressure Liquid - Abstract
The present study was designed to develop a sensitive and selective high performance liquid chromatography–tandem mass spectrometric method for the determination of Camellianin A in HepG2 cells. The extraction of Camellianin A was achieved using 15% trichloroacetic acid and then separated on a C 18 column interfaced with a triple quadrupole tandem mass spectrometer in multiple reaction monitoring mode. The mobile phase was consisted of methanol–water (0.1% formic acid) (55 : 45, V / V ). The total run time was 5.0 min. The method was linear in the concentration range of 0.25–250.0 ng·mL −1 . The lower limit of quantification was 0.25 ng·mL −1 . The intra- and inter-day relative standard deviations of entire concentration range were less than 9.3%. The proposed HPLC-MS/MS method was successfully applied to detect the intracellular concentration of Camellianin A in HepG2 cells.
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- 2016
12. Jiawei Erzhiwan improves menopausal metabolic syndrome by enhancing insulin secretion in pancreatic β cells
- Author
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Mu Zhang, Pei Zhang, Xiao-Jun Xu, Shi-Ping Ma, Xie Zhishen, Feng-Guo Xu, Ping Zhou, and Xiao-Meng Wan
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Estrogen receptor ,medicine.disease_cause ,Rats, Sprague-Dawley ,03 medical and health sciences ,Mice ,Internal medicine ,Insulin-Secreting Cells ,Drug Discovery ,Hyperlipidemia ,Insulin Secretion ,medicine ,Animals ,Humans ,Insulin ,Metabolic Syndrome ,geography ,geography.geographical_feature_category ,business.industry ,Antagonist ,General Medicine ,medicine.disease ,Islet ,Rats ,030104 developmental biology ,Endocrinology ,Glucose ,Complementary and alternative medicine ,Ovariectomized rat ,Female ,Metabolic syndrome ,Menopause ,business ,Carcinogenesis ,Hormone ,Drugs, Chinese Herbal - Abstract
Menopausal metabolic syndrome (MMS) is a series of syndrome caused by ovarian function decline and hormone insufficiency, and is a high risk factor for cardiovascular diseases (CVD) and type II diabetes mellitus (T2DM). Erzhiwan (EZW), composed of Herba Ecliptae and Fructus Ligustri Lucidi, is a traditional Chinese herbal formula that has been used to treat menopausal syndrome for many years. We added Herba Epimedii, Radix Rehmanniae, and Fructus Corni into EZW, to prepare a new formula, termed Jiawei Erzhiwan (JE). The present study was designed to determine the anti-MMS effects of JE using ovariectomized (OVX) adult female rats that were treated with JE for 4 weeks, and β-tc-6 cells and INS cells were used to detected the protect effectiveness of JE. Our results showed JE could increase insulin sensitivity and ameliorated hyperlipidemia. Metabolomics analysis showed that the serum levels of branched and aromatic amino acids were down-regulated in serum by JE administration. Moreover, JE enhanced the function of islet β cells INS-1 and β-tc-6, through increasing the glucose stimulated insulin secretion (GSIS), which was abolished by estrogen receptor (ER) antagonist, indicating that JE functions were mediated by ER signaling. Additionally, JE did not induce tumorigenesis in rat mammary tissue or promoted proliferation of MCF-7 and Hela cells. In conclusion, our work demonstrated that JE ameliorated OVX-induced glucose and lipid metabolism disorder through activating estrogen receptor pathway and promoting GSIS in islet β cells, thus indicating that JE could be a safe and effective medication for MMS therapy.
- Published
- 2016
13. Quantitative Evaluation of the Compatibility Effects of Huangqin Decoction on the Treatment of Irinotecan-Induced Gastrointestinal Toxicity Using Untargeted Metabolomics.
- Author
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Dong-Ni Cui, Xu Wang, Jia-Qing Chen, Bo Lv, Pei Zhang, Wei Zhang, Zun-Jian Zhang, and Feng-Guo Xu
- Subjects
ALTERNATIVE medicine ,METABOLOMICS ,GASTROINTESTINAL disease treatment - Abstract
Huangqin decoction (HQD), a traditional Chinese medicine (TCM), has been widely used to treat gastrointestinal syndrome in China for thousands of years. Chemotherapy drug irinotecan (CPT-11) is used clinically to treat various kinds of cancers but limited by its side effects, especially delayed diarrhea. Nowadays, HQD has been proved to be effective in attenuating the intestinal toxicity induced by CPT-11. HQD consists of four medicinal herbs including Scutellaria baicalensis Georgi, Glycyrrhiza uralensis Fisch, Paeonia lactiflora Pall, and Ziziphus jujuba Mill. Due to its complexity, the role of each herb and the multi-herb synergistic effects of the formula are poorly understood. In order to quantitatively assess the compatibility effects of HQD, mass spectrometry-based untargeted metabolomics studies were performed. The serum metabolic profiles of rats administered with HQD, single S. baicalensis decoction, S. baicalensis-free decoction and baicalin/baicalein combination were compared. A time-dependent trajectory upon principal component analysis was firstly used to visualize the overall differences. Then metabolites deregulation score and relative area under the curve were calculated and used as parameters to quantitatively evaluate the compatibility effects of HQD from the aspect of global metabolic profile and the specifically altered metabolites, respectively. The collective results indicated that S. baicalensis played a crucial role in the therapeutic effect of HQD on irinotecan-induced diarrhea. Both HQD and SS decoction regulated glycine, serine and threonine pathway. This study demonstrated that metabolomics was a promising tool to elucidate the compatibility effects of TCM or combinatorial drugs. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
14. HuangQin Decoction Attenuates CPT-11-Induced Gastrointestinal Toxicity by Regulating Bile Acids Metabolism Homeostasis.
- Author
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Xu Wang, Dong-ni Cui, Xiao-min Dai, Jing Wang, Wei Zhang, Zun-jian Zhang, and Feng-guo Xu
- Subjects
IRINOTECAN ,BILE acids ,GASTROINTESTINAL system physiology - Abstract
Irinotecan (CPT-11) is a potent chemotherapeutic agent, however, its clinical usage is often limited by the induction of severe gastrointestinal (GI) toxicity, especially late-onset diarrhea. HuangQin Decoction (HQD), commonly used for the treatment of GI ailments, has been proved could significantly ameliorate the intestinal toxicity of CPT-11. To reveal the mechanisms of CPT-11-induced toxicity and the modulation effects of HQD, a previous untargeted metabolomics study was performed and the results indicated that HQD may protect the GI tract by altering the metabolism of bile acids (BAs). Nevertheless, the untargeted assays are often less sensitive and/or efficient. In order to further confirm our previous findings, here in this paper, serum and tissues metabolic profiles of 17 BAs were analyzed using liquid chromatography-tandem mass spectrometry based targeted metabolomics. The results indicated that serum and tissues levels of most BAs were significantly decreased after CPT-11 administration, except some hydrophobic BAs. Co-treatment with HQD could markedly attenuate CPT-11-induced GI toxicity and reverse the alterations of hydrophobic BAs. Despite the fact that the BAs pool size remained unchanged, the balance of BAs had shifted leading to decreased toxicity after HQD treatment. The present study demonstrated for the first time that the precise interaction between HQD, CPT-11-induced intestinal toxicity and BAs' homeostasis. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
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