3,198 results on '"Feng, Gu"'
Search Results
2. ASIC1/RIP1 accelerates atherosclerosis via disrupting lipophagy
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Yuan-Mei Wang, Huang Tang, Ya-Jie Tang, Juan Liu, Yu-Fang Yin, Ya-Ling Tang, Yao-Guang Feng, and Hong-Feng Gu
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Atherosclerosis ,ASIC1 ,RIP1 ,Lipophagy ,Lipid accumulation ,Medicine (General) ,R5-920 ,Science (General) ,Q1-390 - Abstract
Introduction: Atherosclerosis, a major contributor to cardiovascular disease, remains a significant health concern worldwide. While previous research has shown that acid-sensing ion channel 1 (ASIC1) impedes macrophage cholesterol efflux, its precise role in atherogenesis and the underlying mechanisms have remained elusive. Objectives: This study aimed to investigate the role of ASIC1 in atherosclerosis and its underlying mechanisms. Methods: First, data from a single-cell RNA sequencing (scRNA-seq) database were used to explore the relationships between ASIC1 differential expression and lipophagy in human atherosclerotic lesions. Finally, we validated the role of ASIC1/RIP1 signaling in lipophagy in vivo (human and mice) and in vitro (RAW264.7 and HTP-1 cells). Result: Our results demonstrated a significant increase in ASIC1 protein levels within CD68+ macrophages in both human aortic lesions and AopE-/- mouse lesion areas compared to nonlesion regions. Concurrently, there was a notable decrease in lipophagy, a crucial process for lipid metabolism. In vitro assays further elucidated that ASIC1 interaction with RIP1 (receptor-interacting protein 1) promoted the phosphorylation of RIP1 at serine 166 and transcription factor EB (TFEB) at serine 142, leading to disrupted lipophagy and increased lipid accumulation. Intriguingly, all these events were reversed upon ASIC1 deficiency and RIP1 inhibition. Furthermore, in ApoE-/- mouse models of atherosclerosis, silencing ASIC1 expression or inhibiting RIP1 activation not only significantly attenuated atherogenesis but also restored TFEB-mediated lipophagy in aortic tissues. This was evidenced by reduced TFEB Ser-142 phosphorylation, decreased LC3II and LAMP1 protein expression, increased numbers of lipophagosomes, and a decrease in lipid droplets. Conclusion: Our findings unveil the critical role of macrophage ASIC1 in interacting with RIP1 to inhibit lipophagy, thereby promoting atherogenesis. Targeting ASIC1 represents a promising therapeutic avenue for the treatment of atherosclerosis.
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- 2024
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3. Optimizing nitrogen fertilizer for improved root growth, nitrogen utilization, and yield of cotton under mulched drip irrigation in southern Xinjiang, China
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Luo, Yu, Yin, Hao, Ma, Yue, Wang, Juanhong, Che, Qingxuan, Zhang, Man, Chen, Bolang, and Feng, Gu
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- 2024
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4. Adding plant metabolites improve plant phosphorus uptake by altering the rhizosphere bacterial community structure
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Wang, Siji, Duan, Shilong, George, Timothy S., Feng, Gu, and Zhang, Lin
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- 2024
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5. PEX3 promotes regenerative repair after myocardial injury in mice through facilitating plasma membrane localization of ITGB3
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Jia-Teng Sun, Zi-Mu Wang, Liu-Hua Zhou, Tong-Tong Yang, Di Zhao, Yu-Lin Bao, Si-Bo Wang, Ling-Feng Gu, Jia-Wen Chen, Tian-Kai Shan, Tian-Wen Wei, Hao Wang, Qi-Ming Wang, Xiang-Qing Kong, Li-Ping Xie, Ai-Hua Gu, Yang Zhao, Feng Chen, Yong Ji, Yi-Qiang Cui, and Lian-Sheng Wang
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Biology (General) ,QH301-705.5 - Abstract
Abstract The peroxisome is a versatile organelle that performs diverse metabolic functions. PEX3, a critical regulator of the peroxisome, participates in various biological processes associated with the peroxisome. Whether PEX3 is involved in peroxisome-related redox homeostasis and myocardial regenerative repair remains elusive. We investigate that cardiomyocyte-specific PEX3 knockout (Pex3-KO) results in an imbalance of redox homeostasis and disrupts the endogenous proliferation/development at different times and spatial locations. Using Pex3-KO mice and myocardium-targeted intervention approaches, the effects of PEX3 on myocardial regenerative repair during both physiological and pathological stages are explored. Mechanistically, lipid metabolomics reveals that PEX3 promotes myocardial regenerative repair by affecting plasmalogen metabolism. Further, we find that PEX3-regulated plasmalogen activates the AKT/GSK3β signaling pathway via the plasma membrane localization of ITGB3. Our study indicates that PEX3 may represent a novel therapeutic target for myocardial regenerative repair following injury.
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- 2024
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6. AI-Based Advanced Approaches and Dry Eye Disease Detection Based on Multi-Source Evidence: Cases, Applications, Issues, and Future Directions
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Mini Han Wang, Lumin Xing, Yi Pan, Feng Gu, Junbin Fang, Xiangrong Yu, Chi Pui Pang, Kelvin Kam-Lung Chong, Carol Yim-Lui Cheung, Xulin Liao, Xiaoxiao Fang, Jie Yang, Ruoyu Zhou, Xiaoshu Zhou, Fengling Wang, and Wenjian Liu
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artificial intelligence (ai) ,dry eye disease (ded) detection ,ophthalmology ,multi-source evidence ,Electronic computers. Computer science ,QA75.5-76.95 - Abstract
This study explores the potential of Artificial Intelligence (AI) in early screening and prognosis of Dry Eye Disease (DED), aiming to enhance the accuracy of therapeutic approaches for eye-care practitioners. Despite the promising opportunities, challenges such as diverse diagnostic evidence, complex etiology, and interdisciplinary knowledge integration impede the interpretability, reliability, and applicability of AI-based DED detection methods. The research conducts a comprehensive review of datasets, diagnostic evidence, and standards, as well as advanced algorithms in AI-based DED detection over the past five years. The DED diagnostic methods are categorized into three groups based on their relationship with AI techniques: (1) those with ground truth and/or comparable standards, (2) potential AI-based methods with significant advantages, and (3) supplementary methods for AI-based DED detection. The study proposes suggested DED detection standards, the combination of multiple diagnostic evidence, and future research directions to guide further investigations. Ultimately, the research contributes to the advancement of ophthalmic disease detection by providing insights into knowledge foundations, advanced methods, challenges, and potential future perspectives, emphasizing the significant role of AI in both academic and practical aspects of ophthalmology.
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- 2024
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7. Establishment of an automatic diagnosis system for corneal endothelium diseases using artificial intelligence
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Jing-hao Qu, Xiao-ran Qin, Zi-jun Xie, Jia-he Qian, Yang Zhang, Xiao-nan Sun, Yu-zhao Sun, Rong-mei Peng, Ge-ge Xiao, Jing Lin, Xiao-yan Bian, Tie-hong Chen, Yan Cheng, Shao-feng Gu, Hai-kun Wang, and Jing Hong
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Corneal endothelium disease ,In vivo confocal microscopy ,Enhanced compact convolutional transformer ,Computer engineering. Computer hardware ,TK7885-7895 ,Information technology ,T58.5-58.64 ,Electronic computers. Computer science ,QA75.5-76.95 - Abstract
Abstract Purpose To use artificial intelligence to establish an automatic diagnosis system for corneal endothelium diseases (CEDs). Methods We develop an automatic system for detecting multiple common CEDs involving an enhanced compact convolutional transformer (ECCT). Specifically, we introduce a cross-head relative position encoding scheme into a standard self-attention module to capture contextual information among different regions and employ a token-attention feed-forward network to place greater focus on valuable abnormal regions. Results A total of 2723 images from CED patients are used to train our system. It achieves an accuracy of 89.53%, and the area under the receiver operating characteristic curve (AUC) is 0.958 (95% CI 0.943–0.971) on images from multiple centres. Conclusions Our system is the first artificial intelligence-based system for diagnosing CEDs worldwide. Images can be uploaded to a specified website, and automatic diagnoses can be obtained; this system can be particularly helpful under pandemic conditions, such as those seen during the recent COVID-19 pandemic.
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- 2024
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8. Increased excitatory connectivity and epileptiform activity in thrombospondin1/2 knockout mice following cortical trauma
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Haifeng Shu, Isabel Parada, Alex Delgado, David A. Prince, and Feng Gu
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Thrombospondin ,Excitatory synapses ,Epilepsy ,Cortical injury ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Thrombospondins (TSPs) are astrocyte-secreted extracellular matrix proteins that play key roles as regulators of synaptogenesis in the central nervous system. We previously showed that TSP1/2 are upregulated in the partial neocortical isolation model (“undercut” or “UC” below) of posttraumatic epileptogenesis and may contribute to abnormal axonal sprouting, aberrant synaptogenesis and epileptiform discharges in the UC cortex. These results led to the hypothesis that posttraumatic epileptogeneis would be reduced in TSP1/2 knockout (TSP1/2 KO) mice. To test the hypothesis, we made UC lesions at P21, and subsequent experiments were conducted 14d later at P35. Ex vivo extracellular single or multi-electrode field potential recordings were obtained from layer V in cortical slices at P35 and in vivo video-EEGs of spontaneous epileptiform bursts were recorded to examine the effect of TSP1/2 deletion on epileptogenesis following cortical injury. Immunohistochemical experiments were performed to assess the effect of TSP1/2 KO + UC on the number of putative excitatory synapses and the expression of TSP4 and HEVIN, other astrocytic proteins known to up-regulate excitatory synapse formation. Unexpectedly, our results showed that, compared with WT + UC mice, TSP1/2 KO + UC mice displayed increased epileptiform activity, as indicated by 1) increased incidence and more rapid propagation of evoked and spontaneous epileptiform discharges in UC neocortical slices; 2) increased occurrence of spontaneous epileptiform discharges in vivo. There was an associated increase in the density of VLUT1/PSD95-IR colocalizations (putative excitatory synapses) and significantly upregulated TSP4- and HEVIN-IR in TSP1/2 KO + UC versus WT + UC mice. Results suggest that TSP1/2 deletion plays a potential epileptogenic role following neocortical injury, associated with compensatory upregulation of TSP4 and HEVIN, which may contribute to the increase in the density of excitatory synapses and resulting neural network hyperexcitability.
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- 2024
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9. Efficacy of Danlou tablets in patients with acute coronary syndrome undergoing percutaneous coronary intervention: a multicenter prospective cohort study
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Yajie Cai, Qiaoning Yang, Ruixi Xi, Furong Yang, Feng Gu, Yang Zhao, Ming Guo, Guoju Dong, Zhuye Gao, Changgeng Fu, Peili Wang, Jianpeng Du, Dawu Zhang, Wenhui Duan, Lizhi Li, Dazhuo Shi, and Ruina Bai
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Danlou tablet ,percutaneous coronary intervention ,acute coronary syndrome ,efficacy ,Chinese medicine ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
BackgroundDanlou tablets (DLTs) have been widely used to treat coronary heart disease in China. However, the benefits associated with DLT for patients with acute coronary syndrome (ACS) undergoing percutaneous coronary intervention (PCI) in routine practice require further investigation.PurposeTo investigate the effectiveness of DLT in patients with ACS undergoing PCI.MethodsThis multicenter prospective cohort study for patients with ACS undergoing PCI was conducted in 40 centers in mainland China from February 2012 to December 2018. This trial is registered under ChiCTR-OOC-14005552. Patients were assigned to either the DLT group or the conventional medicine (CM) group based on whether they used DLT prior to enrollment. The duration of DLT use (1.5 g, three times a day) was 12 months. The primary endpoint comprised of cardiac death, non-fatal myocardial infarction, and urgent revascularization. Secondary endpoint included rehospitalization owing to ACS, heart failure, stroke, and other thrombotic events. The Seattle Angina Questionnaire (SAQ) was used to assess quality of life (QOL). Primary and secondary endpoints were followed up for 36 months, and the SAQ was followed up for 12 months. The Cox proportional hazards regression model was used to analyze the independent effect of DLT on primary and secondary endpoints. Propensity score matching (PSM) analyses were performed to mitigate bias. Survival estimation was performed using Kaplan–Meier survival curves and log-rank tests in the PSM cohort, and landmark analyses were used for further evaluation of primary and secondary endpoints. Subgroup analyses and interactions confirmed the robustness of the findings. Linear mixed effects models were used to assess the QOL.ResultsOverall, 936 patients were enrolled in this cohort study, of whom 875 completed follow-up. The primary and secondary endpoints had no significantly difference between the DLT and CM groups after Cox proportional hazards models. Kaplan–Meier survival curves and log-rank tests performed in the PSM cohort also found no significant differences between the two groups on primary and secondary endpoints. However, landmark analysis showed significant benefit in the primary endpoint for the DLT group after 200 days (hazard ratio [HR] 0.46, 95% confidence interval [CI] 0.22–0.93, P = 0.03). Landmark analysis also showed a significant benefit in the secondary endpoint in the DLT group within 200 days (HR 0.33, 95% CI 0.15–0.73, P = 0.006). Moreover, DLT improves the SAQ summary score, and scores in the physical limitation, treatment satisfaction, and disease perception domains for patients with ACS undergoing PCI.ConclusionsDLT combined with conventional treatment reduced the risk of the primary endpoint after 200 days and the secondary endpoint within 200 days during the 3-year follow-up. Additionally, DLT can improve the QOL without adverse effects.
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- 2024
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10. Processing of emotional connotations in Chinese monomorphic and compound words reflected by the early posterior negativity
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Kai Zhang, Jiaxin Li, and Feng Gu
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emotion processing ,Chinese words ,early posterior negativity (EPN) ,event-related potentials (ERPs) ,affective neurolinguistics ,Psychology ,BF1-990 - Abstract
Writing stands as one of humanity’s most profound inventions, facilitating the efficient sharing and transmission of vast amounts of information. Similar to images and facial expressions, visual (written) words possess the ability to evoke emotional connotations. Understanding how the brain perceives these emotional nuances encoded in highly symbolic visual words is a key focus of the emerging field of “affective neurolinguistics.” At the core of this inquiry lies the examination of the early posterior negativity (EPN), an event-related potentials (ERPs) component peaking around 300 ms after stimulus onset in the occipitotemporal scalp region. EPN has consistently emerged in response to emotional stimuli, encompassing pictures, faces, and visual words. However, prior research has notably lacked observation of EPN in response to Chinese emotional words, raising questions about potential differences in emotional processing between Chinese and other languages. Given the logographic nature of the Chinese writing system and the prevalence of compound words in the Chinese lexicon, this study aims to explore whether the emotional processing of Chinese monomorphic and compound words elicits an EPN response. Two experiments were conducted: Experiment 1 utilized one-character words (monomorphic words), while Experiment 2 employed two-character words (compound words). Participants were assigned a go/no-go task, instructed to respond to unknown words (word recognition task) or blue stimuli (color decision task). Data analysis using a data-driven mass univariate approach revealed significant ERP differences between emotional and neutral words. Notably, the time course, scalp topography, and cortical generators of the difference ERP presented a characteristic EPN response in both experiments. These findings strongly support the notion that the processing of emotional connotations in both Chinese monomorphic and compound words is reflected by the EPN, paving the way for future research using EPN as an emotion-related ERP component for investigating emotional processing of Chinese words.
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- 2024
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11. More Victories, Less Cooperation: Assessing Cicero's Diplomacy Play.
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Wichayaporn Wongkamjan, Feng Gu, Yanze Wang, Ulf Hermjakob, Jonathan May, Brandon M. Stewart, Jonathan K. Kummerfeld, Denis Peskoff, and Jordan L. Boyd-Graber
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- 2024
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12. Prediction of blood–brain barrier penetrating peptides based on data augmentation with Augur
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Zhi-Feng Gu, Yu-Duo Hao, Tian-Yu Wang, Pei-Ling Cai, Yang Zhang, Ke-Jun Deng, Hao Lin, and Hao Lv
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Blood–brain barrier ,Penetrating peptides ,Machine learning ,Data augmentation ,Feature selection ,Information gain ,Biology (General) ,QH301-705.5 - Abstract
Abstract Background The blood–brain barrier serves as a critical interface between the bloodstream and brain tissue, mainly composed of pericytes, neurons, endothelial cells, and tightly connected basal membranes. It plays a pivotal role in safeguarding brain from harmful substances, thus protecting the integrity of the nervous system and preserving overall brain homeostasis. However, this remarkable selective transmission also poses a formidable challenge in the realm of central nervous system diseases treatment, hindering the delivery of large-molecule drugs into the brain. In response to this challenge, many researchers have devoted themselves to developing drug delivery systems capable of breaching the blood–brain barrier. Among these, blood–brain barrier penetrating peptides have emerged as promising candidates. These peptides had the advantages of high biosafety, ease of synthesis, and exceptional penetration efficiency, making them an effective drug delivery solution. While previous studies have developed a few prediction models for blood–brain barrier penetrating peptides, their performance has often been hampered by issue of limited positive data. Results In this study, we present Augur, a novel prediction model using borderline-SMOTE-based data augmentation and machine learning. we extract highly interpretable physicochemical properties of blood–brain barrier penetrating peptides while solving the issues of small sample size and imbalance of positive and negative samples. Experimental results demonstrate the superior prediction performance of Augur with an AUC value of 0.932 on the training set and 0.931 on the independent test set. Conclusions This newly developed Augur model demonstrates superior performance in predicting blood–brain barrier penetrating peptides, offering valuable insights for drug development targeting neurological disorders. This breakthrough may enhance the efficiency of peptide-based drug discovery and pave the way for innovative treatment strategies for central nervous system diseases.
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- 2024
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13. Artemisinin ameliorates cognitive decline by inhibiting hippocampal neuronal ferroptosis via Nrf2 activation in T2DM mice
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Bo Wang, Sheng Zhu, Miao Guo, Run-Dong Ma, Ya-Ling Tang, Ya-Xiong Nie, and Hong-Feng Gu
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Diabetic cognitive deficit ,Ferroptosis ,Hippocampus ,Artemisinin ,Nrf2 ,Therapeutics. Pharmacology ,RM1-950 ,Biochemistry ,QD415-436 - Abstract
Abstract Background Neuronal ferroptosis plays a critical role in the pathogenesis of cognitive deficits. The present study explored whether artemisinin protected type 2 diabetes mellitus (T2DM) mice from cognitive impairments by attenuating neuronal ferroptosis in the hippocampal CA1 region. Methods STZ-induced T2DM mice were treated with artemisinin (40 mg/kg, i.p.), or cotreated with artemisinin and Nrf2 inhibitor MEL385 or ferroptosis inducer erastin for 4 weeks. Cognitive performance was determined by the Morris water maze and Y maze tests. Hippocampal ROS, MDA, GSH, and Fe2+ contents were detected by assay kits. Nrf2, p-Nrf2, HO-1, and GPX4 proteins in hippocampal CA1 were assessed by Western blotting. Hippocampal neuron injury and mitochondrial morphology were observed using H&E staining and a transmission electron microscope, respectively. Results Artemisinin reversed diabetic cognitive impairments, decreased the concentrations of ROS, MDA and Fe2+, and increased the levels of p-Nr2, HO-1, GPX4 and GSH. Moreover, artemisinin alleviated neuronal loss and ferroptosis in the hippocampal CA1 region. However, these neuroprotective effects of artemisinin were abolished by Nrf2 inhibitor ML385 and ferroptosis inducer erastin. Conclusion Artemisinin effectively ameliorates neuropathological changes and learning and memory decline in T2DM mice; the underlying mechanism involves the activation of Nrf2 to inhibit neuronal ferroptosis in the hippocampus. Graphical Abstract
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- 2024
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14. Shock wave assisted intracellular delivery of antibiotics against bone infection with Staphylococcus aureus via P2X7 receptors
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Jiangbi Li, Haixia Li, Songqi Bi, Yang Sun, Feng Gu, and Tiecheng Yu
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Chronic osteomyelitis ,Shock wave ,Cell membrane permeability ,Osteoblasts ,P2X7 receptors ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Background: Treatment of chronic osteomyelitis (bone infection) remains a clinical challenge; in particular, it requires enhanced delivery of antibiotic drugs for the treatment of intracellular Staphylococcus aureus (S. aureus), which prevents infection recurrence and resistance. Previous studies have found that noninvasive shock waves used to treat musculoskeletal diseases can alter cell permeability, however, it is unclear whether shock waves alter cell membrane permeability in chronic osteomyelitis. Furthermore, it remains unknown whether such changes in permeability promote the entry of antibiotics into osteoblasts to exert antibacterial effects. Methods: In our study, trypan blue staining was used to determine the shock wave parameters that had no obvious damage to the osteoblast model; the effect of shocks waves on the cell membrane permeability of osteoblast model was detected by BODIPY®FL vancomycin; high performance liquid chromatography-mass spectrometry (HLPC-MS) was used to detect the effect of shock wave on the entry of antibiotics into the osteoblast model; plate colony counting method was used to detect the clearance effect of shock wave assisted antibiotics on S. aureus in the osteoblast model. To explore the mechanism, the effect of different pulses of shock waves on S. aureus was examined by plate colony counting method, besides, P2X7 receptor in osteoblast was detected by immunofluorescence and the extracellular ATP levels was detected. Furthermore, the effect of P2X7 receptor antagonists KN-62 or A740003 on the intracellular antibacterial activity of shock-assisted antibiotics was observed. Then, we used S. aureus to establish a rat model of chronic tibial osteomyelitis and investigated the efficacy and safety of shock-wave assisted antibiotics in the treatment of chronic osteomyelitis in rats. Results: The viability of the osteoblast models of intracellular S. aureus infection was not significantly affected by the application of up to 400 shock wave pulses at 0.21 mJ/mm2. Surprisingly, the delivery of BODIPY®FL vancomycin to osteoblast model cells was markedly enhanced by this shock wave treatment. Furthermore, the shock wave therapy increased the delivery of hydrophilic antibiotics (vancomycin and cefuroxime sodium), but not lipophilic antibiotics (rifampicin and levofloxacin), which improved the intracellular antibacterial effect. Afterwards, we discovered that shock wave treatment increased the extracellular concentration of ATP (the P2X7 receptor activator), while KN-62 or A740003, a P2X7 receptor inhibitor, decreased intracellular antibacterial activity. We then found that 0.1 mL of 1 × 1011 CFU/mL ATCC25923 S. aureus was suitable for modeling chronic osteomyelitis in rats. Besides, the shock wave-assisted vancomycin treatment with the strongest antibacterial and osteogenic effects among the tested treatments was confirmed in vivo by imaging examination, microbiological cultures, and histopathology, with favorable safety. Conclusions: Our results suggest that shock waves can promote the entry of antibiotics into osteoblasts for antibacteria by changing the cell membrane permeability in a P2X7 receptor-dependent manner. Besides, considering antibacterial and osteogenic efficiency and a high degree of safety in rat osteomyelitis model, shock wave-assisted vancomycin treatment may thus represent a possible adjuvant therapy for chronic osteomyelitis.
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- 2024
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15. Research on sand control in thin interbed CBM wells
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Yue ZHANG, Jiaojiao YU, Youwu LI, Xiaodan WU, Shixin JIANG, Feng GU, Jingyun CUI, and Ying GAO
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thin interbed ,coalbed methane well ,sand production ,surat basin ,sand control ,sand carrying production ,Mining engineering. Metallurgy ,TN1-997 - Abstract
Coalbed methane well sand (pulverized coal) problem restricts the production life of coalbed methane well, resulting in high maintenance costs, reduce productivity, and because of the large contact area between thin interbed coal seam and surrounding rock, the problem of sand production (pulverized coal) is prominent. In the Surat Basin of Australia, the coal seam cementation is loose and the coal/mud/sandstone interlayer is complex. In the statistical wells, 100% of the production wells have different degrees of sand production problems, and 33.5% of which have serious sand production, frequent workover operations, and high production costs. Based on the systematic analysis of the reservoir characteristics, sand production characteristics and production rules in Surat Basin, the comprehensive sand discharge prevention technology of “expansion packer + blind tube” and “screw pump + slurry rotor + automatic shunt valve + drilling through casing shoes” is adopted according to the research on the particle settlement law, combined with the prediction of sand production level and the optimization of sand control technology. The problem of sand production in coal measure strata in Surat Basin has been solved in stages. The field application results show that the average continuous production time of a single well increases from 275 days to nearly 400 days, and the workover frequency is reduced by nearly 50%.
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- 2024
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16. Structural insights into histone exchange by human SRCAP complex
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Jiali Yu, Fengrui Sui, Feng Gu, Wanjun Li, Zishuo Yu, Qianmin Wang, Shuang He, Li Wang, and Yanhui Xu
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Cytology ,QH573-671 - Abstract
Abstract Histone variant H2A.Z is found at promoters and regulates transcription. The ATP-dependent chromatin remodeler SRCAP complex (SRCAP-C) promotes the replacement of canonical histone H2A–H2B dimer with H2A.Z–H2B dimer. Here, we determined structures of human SRCAP-C bound to H2A-containing nucleosome at near-atomic resolution. The SRCAP subunit integrates a 6-subunit actin-related protein (ARP) module and an ATPase-containing motor module. The ATPase-associated ARP module encircles half of the nucleosome along the DNA and may restrain net DNA translocation, a unique feature of SRCAP-C. The motor module adopts distinct nucleosome binding modes in the apo (nucleotide-free), ADP-bound, and ADP-BeFx-bound states, suggesting that ATPase-driven movement destabilizes H2A–H2B by unwrapping the entry DNA and pulls H2A–H2B out of nucleosome through the ZNHIT1 subunit. Structure-guided chromatin immunoprecipitation sequencing analysis confirmed the requirement of H2A-contacting ZNHIT1 in maintaining H2A.Z occupancy on the genome. Our study provides structural insights into the mechanism of H2A-H2A.Z exchange mediated by SRCAP-C.
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- 2024
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17. Diversity of Tropical Cyclones Rapid Intensification
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Ke Peng, Yu‐Xun Tian, Juan Fang, Yan Liu, and Jian‐Feng Gu
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tropical cyclone ,rapid intensification ,TC structure ,Geophysics. Cosmic physics ,QC801-809 - Abstract
Abstract The study investigates the rapid intensification (RI) of tropical cyclones (TCs) in the Northwestern Pacific. We found that rapid changes in the maximum wind speed (Vmax) and the minimum central pressure (Pmin) are not always concurrent. RI cases can be categorized into three types: (a) RIv, only Vmax strengthens rapidly; (b) RIp, only Pmin decreases rapidly; (c) RIpv, rapid changes in Vmax and Pmin occur concurrently. At the onset of RI, RIv‐type TCs exhibit the weakest intensity and the smallest size, with deep convection concentrated in the inner‐core region; RIp‐type TCs are characterized by the strongest cyclone intensity and the largest outer‐core size, with strong convection covering the inner‐ and outer‐core regions; RIpv‐type TCs show moderate intensity, size, and convection distribution. For RIpv, significant strengthening of wind profile is concentrated in the inner‐core region, while for RIp it is more prominent in the outer‐core.
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- 2024
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18. MiR-30c suppresses the proliferation, metastasis and polarity reversal of tumor cell clusters by targeting MTDH in invasive micropapillary carcinoma of the breast
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Yunwei Han, Weidong Li, Renyong zhi, Gui Ma, Ang Gao, Kailiang Wu, Hui Sun, Dan Zhao, Yiling Yang, Fangfang Liu, Feng Gu, Xiaojing Guo, Jintang Dong, Shuai Li, and Li Fu
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Breast cancer ,Invasive micropapillary carcinoma (IMPC) ,MiRNA ,Proliferation ,Metastasis ,Polarity reversal ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Purpose: Invasive micropapillary carcinoma (IMPC) of the breast has a high propensity for lymphovascular invasion and axillary lymph node metastasis and displays an ‘inside-out’ growth pattern, but the molecular mechanism of invasion, metastasis and cell polarity reversal in IMPC is unclear. Methods: and Patients: Cell growth curves, tumor sphere formation assays, transwell assays, mouse xenograft model and immunofluorescence were evaluated to investigate the effects of miR-30c and MTDH. Dual luciferase reporter assays was performed to confirm that the MTDH (metadherin) 3′UTR bound to miR-30c. MiRNA in situ hybridization (ISH) and immunohistochemistry (IHC) were carried out on IMPC patient tissues for miR-30c and MTDH expression, respectively. Results: We found miR-30c as a tumor suppressor gene in cell proliferation, metastasis and polarity reversal of IMPC. Overexpression of miR-30c inhibited cell growth and metastasis in vitro and in vivo. MiR-30c could directly target the MTDH 3′UTR. MiR-30c overexpression inhibited breast cancer cell proliferation, invasion and metastasis by targeting MTDH. Moreover, miR-30c/MTDH axis could also regulate cell polarity reversal of IMPC. By ISH and IHC analyses, miR-30c and MTDH were significantly correlated with tumor size, lymph nodule status and tumor grade, the ‘inside-out’ growth pattern, overall survival (OS) and disease-free survival (DFS) in IMPC patients. Conclusions: Overall, miR-30c/MTDH axis was responsible for tumor proliferation, metastasis and polarity reversal. It may provide promising therapeutic targets and prognostic biomarkers for patients with IMPC.
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- 2024
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19. Checkpoint Kinase 1 Stimulates Endogenous Cardiomyocyte Renewal and Cardiac Repair by Binding to Pyruvate Kinase Isoform M2 C‐Domain and Activating Cardiac Metabolic Reprogramming in a Porcine Model of Myocardial Ischemia/Reperfusion Injury
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Tian‐Wen Wei, Tian‐Kai Shan, Hao Wang, Jia‐Wen Chen, Tong‐Tong Yang, Liu‐Hua Zhou, Di Zhao, Jia‐Teng Sun, Si‐Bo Wang, Ling‐Feng Gu, Chong Du, Qi‐Qi Jiang, Rui Sun, Qi‐Ming Wang, Xiang‐Qing Kong, Xiao‐Hu Lu, Hao‐Liang Sun, Yi Xu, Li‐Ping Xie, Ai‐Hua Gu, Feng Chen, Yong Ji, Xue‐Jiang Guo, and Lian‐Sheng Wang
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metabolic reprogramming ,myocardial ischemia/reperfusion injury ,myocardial repair ,recombinant human checkpoint kinase 1 ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Background The regenerative capacity of the adult mammalian hearts is limited. Numerous studies have explored mechanisms of adult cardiomyocyte cell‐cycle withdrawal. This translational study evaluated the effects and underlying mechanism of rhCHK1 (recombinant human checkpoint kinase 1) on the survival and proliferation of cardiomyocyte and myocardial repair after ischemia/reperfusion injury in swine. Methods and Results Intramyocardial injection of rhCHK1 protein (1 mg/kg) encapsulated in hydrogel stimulated cardiomyocyte proliferation and reduced cardiac inflammation response at 3 days after ischemia/reperfusion injury, improved cardiac function and attenuated ventricular remodeling, and reduced the infarct area at 28 days after ischemia/reperfusion injury. Mechanistically, multiomics sequencing analysis demonstrated enrichment of glycolysis and mTOR (mammalian target of rapamycin) pathways after rhCHK1 treatment. Co‐Immunoprecipitation (Co‐IP) experiments and protein docking prediction showed that CHK1 (checkpoint kinase 1) directly bound to and activated the Serine 37 (S37) and Tyrosine 105 (Y105) sites of PKM2 (pyruvate kinase isoform M2) to promote metabolic reprogramming. We further constructed plasmids that knocked out different CHK1 and PKM2 amino acid domains and transfected them into Human Embryonic Kidney 293T (HEK293T) cells for CO‐IP experiments. Results showed that the 1–265 domain of CHK1 directly binds to the 157–400 amino acids of PKM2. Furthermore, hiPSC‐CM (human iPS cell‐derived cardiomyocyte) in vitro and in vivo experiments both demonstrated that CHK1 stimulated cardiomyocytes renewal and cardiac repair by activating PKM2 C‐domain‐mediated cardiac metabolic reprogramming. Conclusions This study demonstrates that the 1–265 amino acid domain of CHK1 binds to the 157–400 domain of PKM2 and activates PKM2‐mediated metabolic reprogramming to promote cardiomyocyte proliferation and myocardial repair after ischemia/reperfusion injury in adult pigs.
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- 2024
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20. Convergence analysis of the time-varying discrete-time Altafini model on signed network.
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Feng Gu and Dongmei Xie
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- 2024
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21. FDFF-Net: A Full-Scale Difference Feature Fusion Network for Change Detection in High-Resolution Remote Sensing Images.
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Feng Gu, Pengfeng Xiao, Xueliang Zhang 0002, Zhenshi Li, and Dilxat Muhtar
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- 2024
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22. A reverse chain search algorithm for the resource constraint in deep space detectors.
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Yu-Heng Qi, Yuan Liu, De-feng Gu, and Ju-Bo Zhu
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- 2024
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23. Symptom-to-balloon time and risk of ventricular arrhythmias in patients with STEMI undergoing percutaneous coronary intervention: The VERY-STEMI study
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Tian-Kai Shan, Ling-Ling Qian, Xu-Dong Han, Bo Deng, Ling-Feng Gu, Ze-Mu Wang, Ye He, Ting Zhu, Peng Jing, Qi-Ming Wang, Zi-Dun Wang, Ru-Xing Wang, Si-Bo Wang, and Lian-Sheng Wang
- Subjects
Ventricular arrhythmia ,Major adverse cardiovascular event ,ST-Segment elevation myocardial infarction ,Symptom-to-balloon time ,Real-world study ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Background: Ventricular arrhythmias (VAs) mainly occur in the early post-myocardial infarction (MI) period. However, studies examining the association between total myocardial ischemia time interval and the risk of new-onset VAs during a long-term follow-up are scarce. Methods: This study (symptom-to-balloon time and VEntricular aRrhYthmias in patients with STEMI, VERY-STEMI study) was a multicenter, observational cohort and real-world study, which included patients with ST-segment elevation MI (STEMI) undergoing percutaneous coronary intervention (PCI). The primary endpoint was cumulative new-onset VAs during follow-up. The secondary endpoints were the major adverse cardiovascular events (MACE) and changes in left ventricular ejection fraction (ΔLVEF, %). Results: A total of 517 patients with STEMI were included and 236 primary endpoint events occurred. After multivariable adjustments, compared to patients with S2BT of 24 h-7d, those with S2BT ≤ 24 h and S2BT > 7d had a lower risk of primary endpoint. RCS showed an inverted U-shaped relationship between S2BT and the primary endpoint, with an S2BT of 68.4 h at the inflection point. Patients with S2BT ≤ 24 h were associated with a lower risk of MACE and a 4.44 increase in LVEF, while there was no significant difference in MACE and LVEF change between the S2BT > 7d group and S2BT of 24 h-7d group. Conclusions: S2BT of 24 h-7d in STEMI patients was associated with a higher risk of VAs during follow-up. There was an inverted U-shaped relationship between S2BT and VAs, with the highest risk at an S2BT of 68.4 h.
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- 2024
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24. Using virtual reality to improve verbal episodic memory in schizophrenia: A proof-of-concept trial
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Bryce J.M. Bogie, Chelsea Noël, Feng Gu, Sébastien Nadeau, Cecelia Shvetz, Hassan Khan, Marie-Christine Rivard, Stéphane Bouchard, Martin Lepage, and Synthia Guimond
- Subjects
Cognitive remediation therapy ,Ecological treatment ,Episodic memory ,Semantic encoding ,Schizophrenia ,Verbal memory ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Background: Schizophrenia is associated with impairments in verbal episodic memory. Strategy for Semantic Association Memory (SESAME) training represents a promising cognitive remediation program to improve verbal episodic memory. Virtual reality (VR) may be a novel tool to increase the ecological validity and transfer of learned skills of traditional cognitive remediation programs. The present proof-of-concept study aimed to assess the feasibility, acceptability, and preliminary efficacy of a VR-based cognitive remediation module inspired by SESAME principles to improve the use of verbal episodic memory strategies in schizophrenia. Methods: Thirty individuals with schizophrenia/schizoaffective disorder completed this study. Participants were randomized to either a VR-based verbal episodic memory training condition inspired by SESAME principles (intervention group) or an active control condition (control group). In the training condition, a coach taught semantic encoding strategies (active rehearsal and semantic clustering) to help participants remember restaurant orders in VR. In the active control condition, participants completed visuospatial puzzles in VR. Attrition rate, participant experience ratings, and cybersickness questionnaires were used to assess feasibility and acceptability. Trial 1 of the Hopkins Verbal Learning Test – Revised was administered pre- and post-intervention to assess preliminary efficacy. Results: Feasibility was demonstrated by a low attrition rate (5.88 %), and acceptability was demonstrated by limited cybersickness and high levels of enjoyment. Although the increase in the number of semantic clusters used following the module did not reach conventional levels of statistical significance in the intervention group, it demonstrated a notable trend with a medium effect size (t = 1.48, p = 0.15, d = 0.54), in contrast to the control group where it remained stable (t = 0.36, p = 0.72, d = 0.13). These findings were similar for the semantic clustering ratio in the intervention (t = 1.61, p = 0.12, d = 0.59) and control (t = 0.36, p = 0.72, d = 0.13) groups. There was no significant change in the number of recalled words in either group following VR immersion. Discussion: This VR intervention was feasible, acceptable, and may be useful for improving the use of semantic encoding strategies. These findings support the use of more ecological approaches for the treatment of cognitive impairments in schizophrenia, such as VR-based cognitive remediation.
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- 2024
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25. Hierarchical gain scheduling based tilt angle guided robust control during mode transition for tilt-rotor unmanned aircraft vehicle
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Hongyu Nie, Feng Gu, and Yuqing He
- Subjects
Electronics ,TK7800-8360 ,Electronic computers. Computer science ,QA75.5-76.95 - Abstract
Tilt-rotor unmanned aircraft vehicle has the potential to combine vertical take-off and landing capability with efficient, high-speed cruise flight. However, the mode transition process is risky owing to the internal continuous time change of aerodynamic and external uncertain wind disturbances. Gain scheduling between two modes is commonly used to achieve mode transition control. However, the optimal scheduling parameters to allocate the manipulated variables for the stable transition have not been determined to data. Focusing on this problem, a gain scheduling-based tilt angle guided robust control method for mode transition is proposed. The dynamic model is first built and analyzed based on a newly developed 360-kg tilt-rotor unmanned aircraft vehicle. Based on this model, the transition guided curve is mapped with respect to velocity and tilt angle, and it is introduced to the gain scheduling method to optimally achieve the allocation of the manipulated variables. Finally, the feasibility and validity are verified in a simulation experiment. Furthermore, the robustness is verified in simulated wind disturbance.
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- 2024
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26. Conventionality matters in Chinese metaphor but not simile comprehension: evidence from event-related potentials
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Yan Yu, Feng Gu, Yongqing Li, and Jianghua Han
- Subjects
metaphor ,simile ,figurative language ,cognitive processing ,ERP ,N400 ,Psychology ,BF1-990 - Abstract
Metaphor and simile, two prevalent forms of figurative language widely employed in daily communication, serve as significant research subjects in linguistics. The Career of Metaphor Theory in cognitive linguistics posits that as conventionality increases, the cognitive mechanisms of metaphor comprehension shift from “comparison” to “categorization.” In line with this notion, prior electrophysiological investigations have revealed that novel metaphors elicit a stronger N400 brain response compared to conventional metaphors. However, the observed N400 difference between conventional and novel metaphors may merely stem from the familiarity contrast between them, as conventional metaphors are typically more familiar than novel ones. To address this dichotomy, the present study not only compared the N400 responses between conventional and novel metaphors but also between conventional and novel similes. While conventional and novel similes differ in familiarity, similar to conventional and novel metaphors, both are processed via “comparison” mechanisms. The results revealed that novel metaphors elicited larger N400 amplitudes compared to conventional metaphors, aligning with previous findings. In contrast, no significant N400 differences were observed between conventional and novel similes, suggesting that familiarity disparity is unlikely to account for N400 distinctions. Our findings imply that conventional and novel metaphors undergo distinct cognitive processing mechanisms (“comparison” versus “categorization”), thereby providing further empirical validation for the Career of Metaphor Theory.
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- 2024
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27. Advanced design strategy in the anode-electrolyte interface for highly reversible aqueous Zn ion batteries
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Xiyun, Hu, Lanlan, Fan, Shixian, Xiong, Feng, Gu, Lei, Cao, and Shufen, Wang
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- 2024
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28. Classifications of anterior segment structure of congenital corneal opacity in infants and toddlers by ultrasound biomicroscopy and slit-lamp microscopic photographs: an observational study
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Jing Hong, Zijun Xie, Xin Wang, Ting Yu, Siyi Ma, Hanzhi Ben, and Shao-feng Gu
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Corneal opacity ,Cornea diseases ,Congenital abnormalities ,Ultrasound Biomicroscopy ,Ophthalmology ,RE1-994 - Abstract
Abstract Background The structural features have an impact on the surgical prognosis for congenital corneal opacity (CCO). The structural classification system of CCO, however, is lacking. Based on data from ultrasound biomicroscopy (UBM) findings in infants and toddlers with CCO, this research proposed a classification system for the anterior segment structure severity. Methods Medical records, preoperative UBM images and slit-lamp photographs of infants and toddlers diagnosed with CCO at University Third Hospital between December 2018 and June 2022 were reviewed. According to the anterior segment structural features observed in UBM images, eyes were classified as follows: U1, opaque cornea only; U2, central anterior synechia; U3, peripheral anterior synechia combined with angle closure; and U4, aniridia or lens anomaly. The opacity appearance and corneal vascularization density observed in slit-lamp photographs were assigned grades according to previous studies. The extent of vascularization was also recorded. The corresponding intraocular anomaly classifications and ocular surface lesion severity were analysed. Results Among 81 eyes (65 patients), 41 (50.6%) were right eyes, and 40 (49.4%) were left eyes. The median age at examination was 6.91 months (n = 81, 1.00, 34.00). Two (2.5%) of the 81 eyes were classified as U1, 20 (24.7%) as U2, 22 (27.2%) as U3a, 11 (13.6%) as U3b and 26 (32.1%) as U4. Bilateral CCO eyes had more severe UBM classifications (P = 0.019), more severe dysgenesis (P = 0.012) and a larger angle closure (P = 0.009). Eyes with more severe UBM classifications had higher opacity grades (P = 0.003) and vascularization grades (P = 0.014) and a larger vascularization extent (P = 0.001). Eyes with dysgenesis had higher haze grades (P = 0.012) and more severe vascularization (P = 0.003 for density; P = 0.008 for extent), while the angle closure range was related to haze grade (P = 0.013) and vascularization extent (P = 0.003). Conclusions This classification method based on UBM and slit-lamp photography findings in the eyes of CCO infants and toddlers can truly reflect the degree of abnormality of the ocular surface and anterior segment and is correlated with the severity of ocular surface anomalies. This method might provide meaningful guidance for surgical procedure design and prognostic determinations for keratoplasty in CCO eyes.
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- 2024
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29. The FDA-approved anti-amyloid-β monoclonal antibodies for the treatment of Alzheimer’s disease: a systematic review and meta-analysis of randomized controlled trials
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Wenxue Wu, Yi Ji, Zilan Wang, Xiaoxiao Wu, Jiaxuan Li, Feng Gu, Zhouqing Chen, and Zhong Wang
- Subjects
Alzheimer’s disease ,Aducanumab ,Lecanemab ,Anti-amyloid-β monoclonal antibodies ,Medicine - Abstract
Abstract Background Alzheimer’s disease (AD) is a worldwide public health problem and is difficult to cure. Drugs aimed at slowing the progression of the disease have been developed, with the Food and Drug Administration (FDA) granting accelerated approval for aducanumab on June 21, 2021 and a new accelerated approval for lecanemab on January 22, 2023. We performed this systematic review and meta-analysis to assess the efficacy and safety of FDA-approved anti-amyloid-β (anti-Aβ) monoclonal antibodies (mabs) for the treatment of AD. Method PubMed, Embase, and Cochrane Library were systematically searched to identify relevant studies published before May 2023. Efficacy outcomes included Aβ, neuroimaging, and biomarker outcomes. Safety outcomes included amyloid-related imaging abnormalities with edema or effusions (ARIA-E) and ARIA with cerebral microhemorrhages, cerebral macrohemorrhages, or superficial siderosis (ARIA-H). Review Manager 5.4 software was used to assess the data. The standard mean differences (SMDs) or odds ratio (OR) with 95% confidence interval (95% CI) were analyzed and calculated with a random effect model or a fixed effect model. Result Overall, 4471 patients from 6 randomized controlled trials (RCTs), with 2190 patients in the treatment group and 2281 patients in the placebo group meeting the inclusion criteria. FDA-approved anti-Aβ mabs showed statistically significant improvements in clinical outcomes, including CDR-SB (P = 0.01), ADCS-ADL-MCI (P = 0.00003), ADCOMS (P
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- 2023
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30. Comprehensive energy system optimization using developed coyote algorithm for effective management of battery, heat source, and thermal storage
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Yao Hu, Liguang Qiao, Feng Gu, and Gholamreza Fathi
- Subjects
Building energy system ,Heat source battery ,Developed coyote optimization algorithm ,Thermal energy storage ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 - Abstract
The modern building sector faces the challenge of meeting energy demands while minimizing environmental impacts and promoting energy efficiency. This research paper presents a comprehensive approach to optimizing building energy systems using a modified metaheuristic, the Developed Coyote Algorithm (DCO). The increasing importance of storage equipment in energy organizations, driven by changes in peak-load demand and the growing adoption of renewable energy sources, necessitates efficient storage solutions. Battery Energy Storage (BES) and Thermal Energy Storage (TES) are commonly used to store excess energy generated from renewable sources and supply it during peak demand. By applying the DCO algorithm, the operational plans of energy systems comprising BES, air-source heat pumps, and TES can be efficiently optimized with minimal computational requirements. The proposed method aims to enhance the productivity and sustainability of energy systems while providing valuable insights for policymakers and stakeholders involved in renewable energy development. The discharge of batteries and thermal energy storage occurs during low electricity prices, with charging power fluctuating between 100 and 150 kW. GWO and IWWO results show that charging is done at night with 150 kW, with a reduced amount used during other times. Thermal energy storage can store energy from 500 to 800 kW during night time, while DCO generates 700–900 kW. The results of this research contribute to the effective management of batteries, heat sources, and thermal storage in building energy systems, further advancing the utilization of renewable energy resources.
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- 2023
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31. Silicone rubber sealed channel induced self-healing of large bone defects: Where is the limit of self-healing of bone?
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Feng Gu, Ke Zhang, Wan-an Zhu, Zhenjiang Sui, Jiangbi Li, Xiaoping Xie, and Tiecheng Yu
- Subjects
Bone defect ,Regeneration ,Self-healing ,Silicone rubber ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Background: Large defects of long tubular bones due to severe trauma, bone tumor resection, or osteomyelitis debridement are challenging in orthopedics. Bone non-union and other complications often lead to serious consequences. At present, autologous bone graft is still the gold standard for the treatment of large bone defects. However, autologous bone graft sources are limited. Silicon rubber (SR) materials are widely used in biomedical fields, due to their safety and biocompatibility, and even shown to induce nerve regeneration. Materials and methods: We extracted rat bone marrow mesenchymal stem cells (BMMSCs) in vitro and verified the biocompatibility of silicone rubber through cell experiments. Then we designed a rabbit radius critical sized bone defect model to verify the effect of silicone rubber sealed channel inducing bone repair in vivo. Results: SR sealed channel could prevent the fibrous tissue from entering the fracture end and forming bone nonunion, thereby inducing self-healing of long tubular bone through endochondral osteogenesis. The hematoma tissue formed in the early stage was rich in osteogenesis and angiogenesis related proteins, and gradually turned into vascularization and endochondral osteogenesis, and finally realized bone regeneration. Conclusions: In summary, our study proved that SR sealed channel could prevent the fibrous tissue from entering the fracture end and induce self-healing of long tubular bone through endochondral osteogenesis. In this process, the sealed environment provided by the SR channel was key, and this might indicate that the limit of self-healing of bone exceeded the previously thought. The translational potential of this article: This study investigated a new concept to induce the self-healing of large bone defects. It could avoid trauma caused by autologous bone extraction and possible rejection reactions caused by bone graft materials. Further research based on this study, including the innovation of induction materials, might invent a new type of bone inducing production, which could bring convenience to patients. We believed that this study had significant meaning for the treatment of large bone defects in clinical practice.
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- 2023
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32. Advanced Risk Assessment for Deep Excavation in Karst Regions Using Improved Dempster–Shafer and Dynamic Bayesian Networks
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Zhenyu Lei, Yanhong Wang, Yu Zhang, Feng Gu, Zihui Zan, Yuan Mei, Wenzhan Liu, and Dongbo Zhou
- Subjects
deep foundation pit ,dynamic risk assessment ,fuzzy dynamic Bayesian network ,improved evidence theory ,Building construction ,TH1-9745 - Abstract
This study presents a novel risk-assessment methodology for deep foundation pit projects in karst regions, aimed at enhancing project safety and decision-making processes. This approach amalgamates fuzzy dynamic Bayesian networks with a refined Dempster–Shafer (DS) evidence theory to tackle the intricate uncertainties present in such contexts. A comprehensive risk index system, derived from historical accident cases, relevant standards, and the literature, encompasses environmental, design, construction, and management factors. Initial probabilities for each risk factor are determined through the integration of expert knowledge and fuzzy theory. The enhanced Dempster–Shafer theory is utilized to fuse diverse information sources, culminating in a robust and dynamic risk evaluation model. This model leverages real-time monitoring data to dynamically assess and adjust risk levels throughout the construction process. The validation of the proposed method is demonstrated through a detailed case study of the Guangzhou Tangxi Section 1 deep foundation pit project, which effectively identified critical risk factors and facilitated proactive construction strategy adjustments. To further evaluate the reliability of the methodology, comparisons were made with three alternative methods, and applications were conducted on three additional deep foundation pit projects. These comparative analyses confirm the superior reliability and applicability of the proposed methodology across varied scenarios.
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- 2024
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33. A comprehensive evaluation of the optimum amount of phosphate fertilizer for drip irrigation of cotton under mulch based on root morphology, physiology, and mycorrhizal symbiosis
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Chen, Bolang, Ye, Zupeng, Tang, Xuexia, Chai, Zhongping, Ma, Yue, Sheng, Jiandong, and Feng, Gu
- Published
- 2024
- Full Text
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34. Dynamics between soil fixation of fertilizer phosphorus and biological phosphorus mobilization determine the phosphorus budgets in agroecosystems
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Li, Yanlong, Xu, Zhen, Zhang, Lin, Chen, Wangzun, and Feng, Gu
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- 2024
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35. Maximising cotton phosphorus utilisation for zero surplus and high yields: A review of innovative P management strategies
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Peng, Yi, Huo, Weige, and Feng, Gu
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- 2024
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36. Identifying the predictors of mycorrhizal response under multiple fertilization regimes
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Qin, Zefeng, Tian, Yinghao, Hao, Wenying, Zhang, Junling, Feng, Gu, Christie, Peter, and Gai, Jingping
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- 2024
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37. Novel screw-cable integrated system(SCIS) for minimally invasive treatment of patella transverse fractures: a finite element analysis
- Author
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Songyang Liu, Shen Liu, Feng Gu, Xing Wei, and Yonghui Liang
- Subjects
Modified tension band wiring ,SCIS ,Patella transverse fractures ,Finite element analysis ,Orthopedic surgery ,RD701-811 ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Abstract Background The most recommended method for treating transverse patella fractures is modified tension band wiring (MTBW). However, the optimal instrument for use with MTBW is still undetermined. Hence, we aimed to design a novel screw-cable integrated system (SCIS) and compare its biomechanical characteristics with Kirschner-wire, SCIS, and Cable-Pin systems in treating transverse patellar fracture. Methods A finite-element (FE) model of transverse patella fracture was created. The fracture model was fixed with either K-wire, SCIS, or Cable-pin. Different tension force loading (400 N and 800 N), direction(0° and 45°), and screw or K-wire depth(5 mm and 10 mm) were set. The maximum displacement of the fragment and maximum gap opening were measured by using FE analysis. Results Compared with the K-wire and Cable-pin system, SCIS increased the stability of the fractured patella by reducing fragment displacement and gap opening. Under 400 N loading in the direction 45°, SCIS with screw placing at 5-mm depth reduced the maximum fragment displacement (0.43 mm) by 49.62% and 26%, respectively, compared with the K-wire (0.22 mm) and Cable-pin (0. 22 mm) group. Meanwhile, the gap opening in SCIS (0.05 mm) was reduced by 83% and 59.8% (0.05 to 0.18) compared with the K-wire (0.30 mm) and Cable-pin (0.18 mm) group. Conclusion SCIS demonstrated improved biomechanical stability for treating transverse patellar fractures compared to MTBW with Kirschner wire and the Cable-Pin system. Finite element analysis showed SCIS substantially reduced fracture fragment displacement and gap opening under various loading conditions.
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- 2023
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38. Fatigue Damage Detection in SLM-Formed Alloy Layers Based on Nonlinear Ultrasonic Technology
- Author
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ZHANG Xuemin, WANG Feng, GU Li
- Subjects
nonlinear ultrasonic ,selective laser melting ,fatigue damage ,fatigue cycle ,second harmonic ,Materials of engineering and construction. Mechanics of materials ,TA401-492 ,Technology - Abstract
Utilizing the ultrasonic nonlinear coefficient, the fatigue damage state of specimens can be accurately determined. This study rapidly assessed the fatigue damage of selective laser melting(SLM) GH4169 nickel-based alloy layers through higher harmonic detection techniques. Results showed that obvious second harmonics were detected when the frequency increased to 10MPa, and the second harmonics formed an amplitude lower than the fundamental wave. Compared with the initial specimen, the amplitude signal was more obvious in the specimen after 20,000 fatigue tests. There were equiaxed crystals, fusion lines, and columnar crystals in the samples, and many columnar crystals were arranged in regular directions. After the fatigue test, there were many fatigue cracks in the substrate, and the second harmonic amplitude also increased significantly. With the extension of the fatigue period, the β/β0 ratio increased. Different from test point 1, the ultrasonic nonlinear coefficients at point 2 and point 3 only showed a small increase trend relative to the fatigue cycle number. The research has a good theoretical support value for improving the fatigue damage detection accuracy of laser melting samples.
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- 2023
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39. A trade-off between space exploration and mobilization of organic phosphorus through associated microbiomes enables niche differentiation of arbuscular mycorrhizal fungi on the same root
- Author
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Zhou, Jiachao, Kuyper, Thomas W., and Feng, Gu
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- 2023
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40. CFD analysis of iodine mass transfer coupled with a chemical reaction in a filtered containment venting system
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Humayun, Muhammad Shummas, Ali, Majid, Qureshi, Muhammad Uzair, Khan Niazi, Muhammad Bilal, Changqi, Yan, Zongning, Sun, Feng, Gu Hai, and Zhou, Yanmin
- Published
- 2024
- Full Text
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41. A Neighbor-Searching Discrepancy-based Drift Detection Scheme for Learning Evolving Data.
- Author
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Feng Gu, Jie Lu 0001, Zhen Fang 0001, Kun Wang, and Guangquan Zhang 0001
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- 2024
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42. LHRS-Bot: Empowering Remote Sensing with VGI-Enhanced Large Multimodal Language Model.
- Author
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Dilxat Muhtar, Zhenshi Li, Feng Gu, Xueliang Zhang 0002, and Pengfeng Xiao
- Published
- 2024
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43. Robust Control of An Aerial Manipulator Based on A Variable Inertia Parameters Model.
- Author
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Guangyu Zhang 0003, Yuqing He, Bo Dai 0004, Feng Gu 0004, Jianda Han, and Guangjun Liu
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- 2024
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44. A Modified BiSeNet for Spinal Segmentation.
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Yunjiao Deng, Feng Gu, Shuai Wang, Daxing Zeng, Junyan Lu, Haitao Liu 0003, Yulei Hou, and Qinghua Zhang
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- 2023
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45. A Hybrid Resampling Technique with Adaptive Intervals Used in the Parallel/Distributed Particle Filters.
- Author
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Xudong Zhang 0004, Feng Gu 0001, Wei Zhong, and Chunyu Ai
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- 2023
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46. Research on cross-regional path planning algorithm for aircraft unmanned tractor based on improved A* algorithm.
- Author
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Wang Zheng, Wang Xin, Feng Gu, and Lin Sun
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- 2023
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47. Combined Application of Calcium-Magnesium Phosphate Fertilizer with Soluble Phosphorus Improves Maize Growth in a Calcareous Soil
- Author
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Yang, Zihang, Zhou, Wenli, Sun, Baoru, Rengel, Zed, Feng, Gu, and Zhang, Lin
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- 2023
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48. Quantitative Analysis of Traditional Chinese Color
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Yushu Chen and Feng Gu
- Subjects
chinese traditional color ,natural color system ,perceptual image ,correlation test ,Biotechnology ,TP248.13-248.65 - Abstract
Chinese traditional colors are an important component of traditional culture, containing profound national culture and unique aesthetic ideas. Analyzing and applying the color characteristics of traditional Chinese colors can integrate traditional color design concepts into contemporary design, implying a design path from tradition to the present, and meeting the diversified and personalized consumer needs of the market. This study examined consumers' psychological perception of traditional colors using the Natural Color System and Likert scale. Factor analysis and association tests revealed color attributes and image cognitive traits. The results reveal that blackness often degrades color image judgement. Saturation influenced Tang and Qing color perception. Red, green, and blue are the ideal hues for the Tang, Song, and Qing Dynasties, respectively. Song Dynasty culture and emotions are perfectly reflected in the green color. The Ming Dynasty's visual traits are blue, and its culture and feeling are yellow. The red color best reflects the Qing Dynasty's cultural and emotional traits, and the blue hue best matches its aesthetic traits. The study attempts to quantify customised furniture color design and improve product color design's cultural meaning and aesthetic effect.
- Published
- 2023
49. Circular RNA IGF1R Promotes Cardiac Repair via Activating β-Catenin Signaling by Interacting with DDX5 in Mice after Ischemic Insults
- Author
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Tian-Kai Shan, Tong-Tong Yang, Peng Jing, Yu-Lin Bao, Liu-Hua Zhou, Ting Zhu, Xin-Ying Shi, Tian-Wen Wei, Si-Bo Wang, Ling-Feng Gu, Jia-Wen Chen, Ye He, Ze-Mu Wang, Qi-Ming Wang, Li-Ping Xie, Ai-Hua Gu, Yang Zhao, Yong Ji, Hao Wang, and Lian-Sheng Wang
- Subjects
Science - Abstract
The potential of circular RNAs (circRNAs) as biomarkers and therapeutic targets is becoming increasingly evident, yet their roles in cardiac regeneration and myocardial renewal remain largely unexplored. Here, we investigated the function of circIGF1R and related mechanisms in cardiac regeneration. Through analysis of circRNA sequencing data from neonatal and adult cardiomyocytes, circRNAs associated with regeneration were identified. Our data showed that circIGF1R expression was high in neonatal hearts, decreased with postnatal maturation, and up-regulated after cardiac injury. The elevation was validated in patients diagnosed with acute myocardial infarction (MI) within 1 week. In human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) and myocardial tissue from mice after apical resection and MI, we observed that circIGF1R overexpression enhanced cardiomyocyte proliferation, reduced apoptosis, and mitigated cardiac dysfunction and fibrosis, while circIGF1R knockdown impeded endogenous cardiac renewal. Mechanistically, we identified circIGF1R binding proteins through circRNA precipitation followed by mass spectrometry. RNA pull-down Western blot and RNA immunoprecipitation demonstrated that circIGF1R directly interacted with DDX5 and augmented its protein level by suppressing ubiquitin-dependent degradation. This subsequently triggered the β-catenin signaling pathway, leading to the transcriptional activation of cyclin D1 and c-Myc. The roles of circIGF1R and DDX5 in cardiac regeneration were further substantiated through site-directed mutagenesis and rescue experiments. In conclusion, our study highlights the pivotal role of circIGF1R in facilitating heart regeneration and repair after ischemic insults. The circIGF1R/DDX5/β-catenin axis emerges as a novel therapeutic target for enhancing myocardial repair after MI, offering promising avenues for the development of regenerative therapies.
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- 2024
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50. Lycium barbarum polysaccharide alleviates DSS-induced chronic ulcerative colitis by restoring intestinal barrier function and modulating gut microbiota
- Author
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Zhi-Yu Li, Lan-Hui Lin, He-Jun Liang, Ya-Qi Li, Fu-Qian Zhao, Ting-Yi Sun, Zi-Yu Liu, Jing-Yi Zhu, Feng Gu, Jia-Ning Xu, Qi-Yuan Hao, De-Shan Zhou, and Hui-Hong Zhai
- Subjects
Lycium barbarum polysaccharides ,ulcerative colitis ,tight junction proteins ,gut microbiota ,Medicine - Abstract
AbstractPurpose This study examined the protective effects and mechanism of Lycium barbarum polysaccharides (LBP) in the context of intestinal barrier function and intestinal microbiota in mice with dextran sulfate sodium (DSS)-induced chronic ulcerative colitis (UC).Methods C57BL/6J male mice were assigned to a standard normal diet without DSS (control group), a normal diet with DSS (DSS group, 2% DSS given discontinuously for 3 weeks) or a normal diet supplemented with LBP (1% dry feed weight, LBP group, 2% DSS given discontinuously for 3 weeks) for a total of 8 weeks, at which point colonic tissues and caecal contents were collected.Results LBP exerted a significant effect against colitis by increasing body weight, colon length, DAI and histopathological scores. LBP inhibited proinflammatory cytokines (IL-1β, IL-6, iNOS and TNF-α) expression, improved anti-inflammatory cytokine (IL-10) expression, promoted the expression of tight junction proteins (Occludin and ZO-1) via nuclear factor erythroid 2-related factor 2 (Nrf2) activation and decreased Claudin-2 expression to maintain the intestinal mucosal barrier. In addition, the abundances of some probiotics (Ruminococcaceae, Lactobacillus, Butyricicoccus, and Akkermansia) were decreased with DSS treatment but increased obviously with LBP treatment. And LBP reduced the abundance of conditional pathogens associated with UC (Mucispirillum and Sutterella). Furthermore, LBP improved the production of short-chain fatty acids (SCFAs), including acetic acid, propionic acid, butyric acid and isobutyric acid.Conclusion LBP can alleviate DSS-induced UC by regulating inflammatory cytokines and tight junction proteins. Moreover, LBP promotes probiotics, suppresses conditional pathogens and increases SCFAs production, showing a strong prebiotic effect.
- Published
- 2023
- Full Text
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