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22 results on '"Fellner, Michaela"'

1. Alkylamine-tethered molecules recruit FBXO22 for targeted protein degradation

Author

Kagiou, Chrysanthi, Cisneros, Jose A., Farnung, Jakob, Liwocha, Joanna, Offensperger, Fabian, Dong, Kevin, Yang, Ka, Tin, Gary, Horstmann, Christina S., Hinterndorfer, Matthias, Paulo, Joao A., Scholes, Natalie S., Sanchez Avila, Juan, Fellner, Michaela, Andersch, Florian, Hannich, J. Thomas, Zuber, Johannes, Kubicek, Stefan, Gygi, Steven P., Schulman, Brenda A., and Winter, Georg E.

Published
2024
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2. EVI1 drives leukemogenesis through aberrant ERG activation

Author

Schmoellerl, Johannes, Barbosa, Inês A.M., Minnich, Martina, Andersch, Florian, Smeenk, Leonie, Havermans, Marije, Eder, Thomas, Neumann, Tobias, Jude, Julian, Fellner, Michaela, Ebert, Anja, Steininger, Monika, Delwel, Ruud, Grebien, Florian, and Zuber, Johannes

Published
2023
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6. The histone chaperone CAF-1 safeguards somatic cell identity.

Author

Cheloufi, Sihem, Elling, Ulrich, Hopfgartner, Barbara, Jung, Youngsook L, Murn, Jernej, Ninova, Maria, Hubmann, Maria, Badeaux, Aimee I, Euong Ang, Cheen, Tenen, Danielle, Wesche, Daniel J, Abazova, Nadezhda, Hogue, Max, Tasdemir, Nilgun, Brumbaugh, Justin, Rathert, Philipp, Jude, Julian, Ferrari, Francesco, Blanco, Andres, Fellner, Michaela, Wenzel, Daniel, Zinner, Marietta, Vidal, Simon E, Bell, Oliver, Stadtfeld, Matthias, Chang, Howard Y, Almouzni, Genevieve, Lowe, Scott W, Rinn, John, Wernig, Marius, Aravin, Alexei, Shi, Yang, Park, Peter J, Penninger, Josef M, Zuber, Johannes, and Hochedlinger, Konrad

Subjects
Cells, Cultured, Chromatin, Heterochromatin, Nucleosomes, Animals, Mice, Transduction, Genetic, Gene Expression Regulation, RNA Interference, Chromatin Assembly Factor-1, Cellular Reprogramming, Cells, Cultured, Transduction, Genetic, General Science & Technology
Abstract

Cellular differentiation involves profound remodelling of chromatic landscapes, yet the mechanisms by which somatic cell identity is subsequently maintained remain incompletely understood. To further elucidate regulatory pathways that safeguard the somatic state, we performed two comprehensive RNA interference (RNAi) screens targeting chromatin factors during transcription-factor-mediated reprogramming of mouse fibroblasts to induced pluripotent stem cells (iPS cells). Subunits of the chromatin assembly factor-1 (CAF-1) complex, including Chaf1a and Chaf1b, emerged as the most prominent hits from both screens, followed by modulators of lysine sumoylation and heterochromatin maintenance. Optimal modulation of both CAF-1 and transcription factor levels increased reprogramming efficiency by several orders of magnitude and facilitated iPS cell formation in as little as 4 days. Mechanistically, CAF-1 suppression led to a more accessible chromatin structure at enhancer elements early during reprogramming. These changes were accompanied by a decrease in somatic heterochromatin domains, increased binding of Sox2 to pluripotency-specific targets and activation of associated genes. Notably, suppression of CAF-1 also enhanced the direct conversion of B cells into macrophages and fibroblasts into neurons. Together, our findings reveal the histone chaperone CAF-1 to be a novel regulator of somatic cell identity during transcription-factor-induced cell-fate transitions and provide a potential strategy to modulate cellular plasticity in a regenerative setting.

Published
2015

7. Inhibition of CBP synergizes with the RNA-dependent mechanisms of Azacitidine by limiting protein synthesis

Author

Diesch, Jeannine, Le Pannérer, Marguerite-Marie, Winkler, René, Casquero, Raquel, Muhar, Matthias, van der Garde, Mark, Maher, Michael, Herráez, Carolina Martínez, Bech-Serra, Joan J., Fellner, Michaela, Rathert, Philipp, Brooks, Nigel, Zamora, Lurdes, Gentilella, Antonio, de la Torre, Carolina, Zuber, Johannes, Götze, Katharina S., and Buschbeck, Marcus

Published
2021
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8. Precision RNAi using synthetic shRNAmir target sites

Author

Hoffmann, Thomas, primary, Hörmann, Alexandra, primary, Corcokovic, Maja, primary, Zmajkovic, Jakub, additional, Hinterndorfer, Matthias, additional, Salkanovic, Jasko, additional, Spreitzer, Fiona, additional, Köferle, Anna, additional, Gitschtaler, Katrin, additional, Popa, Alexandra, additional, Oberndorfer, Sarah, additional, Andersch, Florian, additional, Schaefer, Markus, additional, Fellner, Michaela, additional, Budano, Nicole, additional, Ruppert, Jan G, additional, Chetta, Paolo, additional, Wurm, Melanie, additional, Zuber, Johannes, additional, and Neumüller, Ralph A, additional

Published
2023
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9. Author Response: Precision RNAi using synthetic shRNAmir target sites

Author

Hoffmann, Thomas, primary, Hörmann, Alexandra, additional, Corcokovic, Maja, additional, Zmajkovic, Jakub, additional, Hinterndorfer, Matthias, additional, Salkanovic, Jasko, additional, Spreitzer, Fiona, additional, Köferle, Anna, additional, Gitschtaler, Katrin, additional, Popa, Alexandra, additional, Oberndorfer, Sarah, additional, Andersch, Florian, additional, Schaefer, Markus, additional, Fellner, Michaela, additional, Budano, Nicole, additional, Ruppert, Jan G, additional, Chetta, Paolo, additional, Wurm, Melanie, additional, Zuber, Johannes, additional, and Neumüller, Ralph A, additional

Published
2023
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11. Precision RNAi using synthetic shRNAmir target sites

Author

Hoffmann, Thomas, primary, Hörmann, Alexandra, additional, Corcokovic, Maja, additional, Zmajkovic, Jakub, additional, Hinterndorfer, Matthias, additional, Salkanovic, Jasko, additional, Spreitzer, Fiona, additional, Köferle, Anna, additional, Gitschtaler, Katrin, additional, Popa, Alexandra, additional, Oberndorfer, Sarah, additional, Andersch, Florian, additional, Schäfer, Markus, additional, Fellner, Michaela, additional, Budano, Nicole, additional, Ruppert, Jan G., additional, Chetta, Paolo, additional, Wurm, Melanie, additional, Zuber, Johannes, additional, and Neumüller, Ralph A., additional

Published
2022
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13. Systematic genetic analysis of muscle morphogenesis and function in Drosophila

Author

Schnorrer, Frank, Schonbauer, Cornelia, Langer, Christoph C.H., Dietzl, Georg, Novatchkova, Maria, Schernhuber, Katharina, Fellner, Michaela, Azaryan, Anna, Radolf, Martin, Stark, Alexander, Keleman, Krystyna, and Dickson, Barry J.

Subjects
Physiological aspects, Genetic aspects, Research, Drosophila -- Analysis -- Genetic aspects, Embryonic development -- Genetic aspects -- Research, Genes -- Physiological aspects -- Research
Abstract

Muscle biology is an attractive target for analysis by genome-wide transgenic RNAi. The basic cell and developmental biology of muscles is largely conserved from insects to mammals (3-6), and their [...], Systematic genetic approaches have provided deep insight into the molecular and cellular mechanisms that operate in simple unicellular organisms. For multicellular organisms, however, the pleiotropy of gene function has largely restricted such approaches to the study of early embryogenesis. With the availability of genome-wide transgenic RNA interference (RNAi) libraries in Drosophila (1,2), it is now possible to perform a systematic genetic dissection of any cell or tissue type at any stage of the lifespan. Here we apply these methods to define the genetic basis for formation and function of the Drosophila muscle. We identify a role in muscle for 2,785 genes, many of which we assign to specific functions in the organization of muscles, myofibrils or sarcomeres. Many of these genes are phylogenetically conserved, including genes implicated in mammalian sarcomere organization and human muscle diseases.

Published
2010
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14. A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila

Author

Dietzl, Georg, Chen, Doris, Schnorrer, Frank, Su, Kuan-Chung, Barinova, Yulia, Fellner, Michaela, Gasser, Beate, Kinsey, Kaolin, Oppel, Silvia, Scheiblauer, Susanne, Couto, Africa, Marra, Vincent, Keleman, Krystyna, and Dickson, Barry J.

Subjects
Gene mutations -- Research -- Genetic aspects, Gene expression -- Research -- Genetic aspects, Drosophila -- Research -- Genetic aspects, Phenotype -- Research -- Genetic aspects, Environmental issues, Science and technology, Zoology and wildlife conservation, Research, Genetic aspects
Abstract

Author(s): Georg Dietzl [1, 2]; Doris Chen [1]; Frank Schnorrer [2]; Kuan-Chung Su [1]; Yulia Barinova [1]; Michaela Fellner [1, 2]; Beate Gasser [1]; Kaolin Kinsey [1, 2]; Silvia Oppel [...]

Published
2007
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15. Selective Requirement of MYB for Oncogenic Hyperactivation of a Translocated Enhancer in Leukemia

Author

Smeenk, Leonie, primary, Ottema, Sophie, additional, Mulet-Lazaro, Roger, additional, Ebert, Anja, additional, Havermans, Marije, additional, Varea, Andrea Arricibita, additional, Fellner, Michaela, additional, Pastoors, Dorien, additional, van Herk, Stanley, additional, Erpelinck-Verschueren, Claudia, additional, Grob, Tim, additional, Hoogenboezem, Remco M., additional, Kavelaars, François G., additional, Matson, Daniel R., additional, Bresnick, Emery H., additional, Bindels, Eric M., additional, Kentsis, Alex, additional, Zuber, Johannes, additional, and Delwel, Ruud, additional

Published
2021
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16. Abstract PO-132: CaTCH - A barcode-guided CRISPRa-inducible reporter to isolate clones from heterogeneous populations

Author

Umkehrer, Christian, primary, Holstein, Felix, additional, Formenti, Laura, additional, Jude, Julian, additional, Froussios, Kimon, additional, Neumann, Tobias, additional, Cronin, Shona M., additional, Haas, Lisa, additional, Lipp, Jesse, additional, Burkard, Thomas R., additional, Fellner, Michaela, additional, Wiesner, Thomas, additional, Zuber, Johannes, additional, and Obenauf, Anna C., additional

Published
2020
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17. Abstract 6221: Targeting IAP in cancer: BI 891065 a potent small molecule SMAC mimetic that synergizes with immune checkpoint inhibition

Author

Aichinger, Martin, primary, Santoro, Valeria, additional, Slavic-Obradovic, Ksenija, additional, Ruhland, Stefanie, additional, Wernitznig, Andreas, additional, Neudolt, Andrea, additional, Schaefer, Markus, additional, Kallenda, Sabine, additional, Zach, Daniel, additional, Olt, Sabine, additional, Salomon, Carina, additional, Rieser, Sarah, additional, Weissenboeck, Martina, additional, Ebner, Florian, additional, Schlattl, Andreas, additional, De Almeida, Melanie Talata, additional, Langlois, Rebecca, additional, Sykora, Martina, additional, Reschke, Markus, additional, Zichner, Thomas, additional, Gerlach, Daniel, additional, Jude, Julian, additional, Fellner, Michaela, additional, Scharn, Dirk, additional, Kraut, Norbert, additional, Moll, Juergen, additional, Zuber, Johannes, additional, Carotta, Sebastian, additional, Impagnatiello, Maria Antonietta, additional, and Tontsch-Grunt, Ulrike, additional

Published
2020
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18. Isolating live cell clones from barcoded populations using CRISPRa-inducible reporters

Author

Umkehrer, Christian, primary, Holstein, Felix, additional, Formenti, Laura, additional, Jude, Julian, additional, Froussios, Kimon, additional, Neumann, Tobias, additional, Cronin, Shona M., additional, Haas, Lisa, additional, Lipp, Jesse J., additional, Burkard, Thomas R., additional, Fellner, Michaela, additional, Wiesner, Thomas, additional, Zuber, Johannes, additional, and Obenauf, Anna C., additional

Published
2020
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20. High-resolution, high-throughput SNP mapping in Drosophila melanogaster

Author

Chen, Doris, Ahlford, Annika, Schnorrer, Frank, Kalchhauser, Irene, Fellner, Michaela, Viràgh, Erika, Kiss, Istvàn, Syvänen, Ann-Christine, Dickson, Barry J, Chen, Doris, Ahlford, Annika, Schnorrer, Frank, Kalchhauser, Irene, Fellner, Michaela, Viràgh, Erika, Kiss, Istvàn, Syvänen, Ann-Christine, and Dickson, Barry J

Abstract

Single nucleotide polymorphisms (SNPs) are useful markers for genetic mapping experiments in model organisms. Here we report the establishment of a high-density SNP map and high-throughput genotyping assays for Drosophila melanogaster. Our map comprises 27,367 SNPs in common laboratory Drosophila stocks. These SNPs were clustered within 2,238 amplifiable markers at an average density of 1 marker every 50.3 kb, or 6.3 genes. We have also constructed a set of 62 Drosophila stocks, each of which facilitates the generation of recombinants within a defined genetic interval of 1-2 Mb. For flexible, high-throughput SNP genotyping, we used fluorescent tag-array mini-sequencing (TAMS) assays. We designed and validated TAMS assays for 293 SNPs at an average resolution of 391.3 kb, and demonstrated the utility of these tools by rapidly mapping 14 mutations that disrupt embryonic muscle patterning. These resources enable high-resolution high-throughput genetic mapping in Drosophila.

Published
2008
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