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In the article, the author discusses the works and challenges facing trans women artists in Canada by citing the experiences of artists Kama La Mackerel, Kim Ninkuru, and Luis De Filippis. Other topics include the role played by communities in the inclusion of said artists, and the issues faced by said artists like poverty and barriers to accessing medical care, employment and education.

CO-WINNER OF THE 2019 JOURNAL OF CURATORIAL STUDIES EMERGING WRITER AWARDART FOR A NEW UNDERSTANDING: NATIVE VOICES, 1950s TO NOW Curated by Mindy N. Besaw, Candice Hopkins and Manuela Well-Off-Man, Crystal Bridges Museum of American Art, Bentonville, 6 October 2018 – 7 January 2019CO-WINNER OF THE 2019 JOURNAL OF CURATORIAL STUDIES EMERGING WRITER AWARDMAQDALA 1868 Curated by Alexandra Jones, Victoria and Albert Museum, London, 5 April 2018 – 30 June 2019RUNNER-UP FOR THE 2019 JOURNAL OF CURATORIAL STUDIES EMERGING WRITER AWARDLAWRENCE ABU HAMDAN, EARWITNESS THEATRE Curated by Ellen Greig, Chisenhale Gallery, London, 21 September – 9 December 2018JASMINA CIBIC: EVERYTHING THAT YOU DESIRE AND NOTHING THAT YOU FEAR Curated by Cheryl Sim, DHC/ART Foundation for Contemporary Art, Montreal, 25 October 2018 – 3 March 2019FRANÇOISE SULLIVAN Curated by Marc Lanctôt, Musée d’art contemporain de Montréal, Montreal, 20 October 2018 – 20 January 2019SADAÂNE AFIF: THIS IS ORNAMENTAL Curated by Anne Faucheret, Kunsthalle Wien, Vienna, 9 September 2018 – 18 November 2018DAVID HARTT: in the forest Oakville Galleries in Gairloch Gardens, Oakville, 23 September 2018 – 6 January 2019DAYNA MCLEOD, INTIMATE KARAOKE, LIVE AT UTERINE CONCERT HALL Curated by FADO Performance Art Centre, co-presented with SummerWorks Performance Festival, The Theatre Centre, Toronto, 15 August 2018HEAVENLY BODIES: FASHION AND THE CATHOLIC IMAGINATION Curated by Andrew Bolton, Metropolitan Museum of Art, New York, 10 May – 8 October 2018CARSTEN HÖLLER AND STEFANO MANCUSO, THE FLORENCE EXPERIMENT Curated by Arturo Galansino, Palazzo Strozzi, Florence, 19 April – 26 August 2018COME UP TO MY ROOM 2018 Curated by Christophe Jivraj, Jana Macalik and Lukus Toane, Gladstone Hotel, Toronto, 18 – 21 January 2018Antarctic biennale Curated by Alexander Pronomarev and Nadim Samman, Antarctica, various locations, 17 – 27 March 2017LIL MIQUELA @lilmiquela, 2016 – ongoing, www.instagram.com/lilmiquela

Ludwig's angina (LA) is an infection of the submandibular space, first described by Wilhelm Frederick von Ludwig in 1836. It represents an entity difficult to manage due to the rapid progression and difficulty in maintaining airway patency, a major challenge in medical practice, resulting in asphyxia and death in 8–10% of patients.

A picture naming task, the Boston Naming Test, originally developed for spoken language, has been adapted to Italian Sign Language in order to assess the vocabulary of Italian deaf signing children. Thirty deaf and thirty hearing children and adolescents (aged 6–14 years) participated in the study. In the deaf signing group, half of the children attended a bilingual school. Results show no significant differences between deaf and hearing participants in the number of correct responses provided, but do show interesting differences in the lexical accuracy observed for individual items and in error types reported for sign and speech. In the deaf group, children attending a bilingual school performed significantly better than those who did not attend a bilingual programme.

Geometry, e.g., the shape of the environment, can be used by numerous animal species to orientate, but data concerning the mouse are lacking. We addressed the question of whether mice are capable of using geometry for navigating. To test whether aging could affect searching strategies, we compared adult (3- to 5-mo old) and aged (20- to 21-mo old) C57BL/6 male mice. We established a water maze task in which spatial information is provided by one landmark proximal to the target (featural information) and by the rectangular shape of the maze (geometric information). By means of probe trials in which we manipulated the presence of these two kinds of information, we show that adult mice can use both geometry and landmark to orientate. By contrast, aged mice do not use geometry and rely exclusively on the landmark to locate the platform. This study provides the first evidence that mice are capable of using geometric information for orientation and that this ability declines in aged animals.

This article deals with the recruitment and employment of foreign workers in the Italian and Portuguese construction sectors. The two countries show a very similar structure and organisation of the sector. Nevertheless, Italy only ‘imports’ immigrants for the sector whereas Portugal both imports foreign labour and exports construction workers on the European market. On the basis of a comparative analysis of the occupational structure of foreign workers and of interviews with construction employers in the two countries this article examines such differences and discusses how (also with reference to irregular work and the underground economy) and why the construction sector represents a key sector for the economic insertion and adaptation of immigrants in the Mediterranean area. Skills shortages and labour cost issues are highlighted as factors affecting recruitment of foreigners in a sector characterised by a very high level of deregulation and informality, and in which processes of downsizing on the one hand and externalisation on the other have had as an important outcome the nearly exclusive role of subcontracting in the recruitment of semi- and low-skilled workers.

Mitral valve insufficiency (MVI) because of involvement of the anterior mitral leaflet may pose additional risks for late outcome after mitral valve repair, because of more complex techniques. We retrospectively reviewed our experience in patients operated on for isolated anterior mitral leaflet prolapse approached by various techniques.

AbstractA framework has been developed for distributed simulation and design of complex systems, with particular application to complete vehicle systems. An object-oriented architecture has been adopted, enabling the use of distributed and heterogeneous computing resources, custom and commercial legacy software, and different models and optimization tools. The industry standard, Common Object Request Broker Architecture (CORBA), is used to provide network-distribution services. To demonstrate the capabilities of the distributed design framework, this paper presents results of a study conducted to optimize the life of an MI Abrams tank road arm. This study demonstrates the reconfigurability of the design framework, as various computing resources, simulation and analysis tools, and optimization algorithms are employed.

AbstractEnvironmentally friendly ground vehicles with range and performance capabilities surpassing those of conventional vehicles require a careful balance among competing goals for fuel efficiency, performance, and emissions. The research objective here is to integrate hybrid electric vehicle simulations with high-fidelity engine modules, to increase the accuracy of predictions, and to allow design studies in the concept evaluation stage. This paper describes a methodology for integrating vehicle and engine simulations. The feed-forward model of the engine is modified to allow its linking with the vehicle model, and an engine component scaling routine is added to facilitate system sizing studies. A design optimization framework is then used to find the best overall engine size, battery pack, and motor combination for minimum fuel consumption within proposed US government performance criteria.

AbstractA generic framework for designing large, complicated systems is formulated, implemented, and used in the design of mechanical systems, including a pressure vessel, an automotive hybrid powertrain, and a tracked vehicle. The framework supports simulation-based design, distributed and heterogeneous computing resources, custom and legacy simulation and analysis codes, reconfigurability of the design problem, and security of operation across untrusted networks. The framework also facilitates the implementation of methodologies for system design that employ design model partitioning and coordination, as well as a variety of models and search algorithms. Common Object Request Broker Architecture (CORBA) middleware for distributed, object-oriented applications was selected to develop and implement the framework. Framework components include subsystem model, design model, search engine, design model partitioning, design coordination, and user interface.

The core protein of proteoglycans from cartilage is substituted with glycosaminoglycans as well as N- and O-glycosidically linked oligosaccharides. We have taken advantage of the long intracellular half-life of the core protein precursor to the rat chondrosarcoma proteoglycan to study the temporal relationship between the addition of the chondroitin sulfate chains and the O-linked oligosaccharides onto the core protein during the formation of the completed proteoglycan molecule. Chondrocyte cultures were pulsed on day 2 with [6-3H]glucosamine for times ranging from 30-420 min. Media and corresponding 4% zwittergent, 4 M guanidine HCl extracts were then pooled and subjected to dissociative density gradient ultracentrifugation to yield purified proteoglycan monomers which were then subjected to alkaline borohydride treatment. The released chondroitin sulfate chains were then purified by precipitation with 50% (v/v) ethanol. The O-linked oligosaccharide-alditols in the supernatant fractions were purified by molecular sieve chromatography on Bio-Gel P-6, and analyzed after digestion with alpha-neuraminidase and subsequent chromatography on Bio-Gel P-2. The different O-linked oligosaccharide-alditols were identified from their hexosamine and hexosaminitol contents. The kinetics of entry of 3H label into N-acetylgalactosamine of chondroitin sulfate was indistinguishable from that into either N-acetylglucosamine or N-acetylgalactosaminitol residues of the oligosaccharide-alditols, with half-times to linear incorporation of 10-17 min. These results show that initiation as well as completion of the O-linked oligosaccharides on the core protein occurs essentially at the same time that chondroitin sulfate chains are added. The results suggest that these biosynthetic processes occur in the Golgi apparatus during the last few minutes of the total intracellular dwell time (half-time of about 90 min) of the core protein acceptor.

The protein subunit of 100-A filaments constitutes approximately 50% of the cytoskeleton protein of chick fibroblasts. In addition to the 43,000-dalton protein (constitutive actin) common to all cell types, fibroblast cytoskeletons contain a 58,000-dalton protein likely to be the 100-A filament subunit, whereas smooth muscle contains, instead, a 55,000-dalton protein. Additional differences among 100-A filaments are shown by immunofluorescence using antibodies angainst chick fibroblast 58,000-dalton component (anti-F58K) and against chick brain 100-A filament subunits (anti-BF). Anti-F58K binds to 100-A filaments in chick fibroblasts, presumptive myoblasts, chondroblasts, pigment cells, and neurons, but not to 100-A filaments in mouse or human fibroblasts. This antibody stains cables of 100-A filaments induced by sequentially treating cells with cytochalasin B and Colcemid. Anti-BF binds only to neurofilaments and not to 100-A filaments of other cell types studied. Absorption or antibodies with purified subunits from gizzard 100-A filaments eliminates binding of anti-F58K to the filaments of all cell types but does not diminish binding of anti-BF to neurofilaments. Various IgGs also bind nonspecifically to induced cables of 100-A filaments. The problem of nonspecific binding of labeled antibodies, as well as the problem of cell and species specificity of the 100-A filaments, is discussed.

Chondrocytes from the Swarm rat chondrosarcoma were pulse-labeled with [3H]serine for 30 min and chased, in the presence of cycloheximide, for times up to 300 min. The movement of newly synthesized core protein precursor of the proteoglycan through elements of the endoplasmic reticulum and Golgi complex was examined. Rough and smooth microsome fractions were obtained by centrifuging postmitochondrial supernatants from cell homogenates on discontinuous sucrose gradients. The core protein precursor was identified in subcellular fractions by (a) immunoprecipitation with an antiserum directed against the hyaluronate binding region of the core protein and the link protein and (b) its size on polyacrylamide gels. Labeled core protein precursor decreased from the microsomes with a t1/2 of 60 +/- 8 min, nearly the same as for the appearance of label in completed proteoglycan monomer (t1/2 = 58 +/- 13 min), consistent with a precursor-product relationship. After correcting for incomplete recovery of the core protein precursor in the microsomal fractions and for cross-contamination of the smooth microsomes by elements of rough endoplasmic reticulum, the redistribution of core protein precursor and completed proteoglycan in the intracellular compartments and of labeled extracellular proteoglycan were fit to a three-compartment model. A t1/2 of 98 +/- 7 min for the loss of core protein precursor from the rough microsomes and a t1/2 = 10 +/- 4 min for the completed proteoglycan in the intracellular compartment (Golgi and secretory vesicles) was obtained. The data indicate that at least 70% of the intracellular transit time for the core protein precursor is spent in the rough endoplasmic reticulum. The addition of glycosaminoglycan chains followed by secretion from the cell occurs relatively rapidly, occupying less than 30% of the total intracellular dwell time.

The distribution of intermediate filament (IF) subunits during maturation of skeletal myotubes in vitro was examined by immunofluorescence, using antibodies against two different types of chick IF subunits: (a) 58-kdalton subunits of fibroblasts (anti-58K), and (b) 55-kdalton subunits of smooth muscle (anti-55K). Anti-58K bound to a filament network in replicating presumptive myoblasts and fibroblasts, as well as in immature myotubes. The distribution in immature myotubes was in longitudinal filaments throughout the cytoplasm. With maturation, staining of myotubes by anti-58K diminished and eventually disappeared. Anti-55K selectively stained myotubes, and the fluorescence localization underwent a drastic change in distribution with maturation--from dense, longitudinal filaments in immature myotubes to a cross-striated distribution in mature myotubes that was associated with the I--Z region of myofibrils. However, the emergence of a cross-striated anti-55K pattern did not coincide temperally with the emergence of striated myofibrils, but occurred over a period of days thereafter.

Myosins synthesized in non-myogenic cells and replicating presumptive myoblasts differ from those synthesized in postmitotic mononucleated myoblasts and myotubes. Myoblasts and myotubes synthesize the definitive light chains, MLC1 and MLC2. These light chains display different molecular weights in sodium dodecyl sulfate-polyacrylamide gels from the fibroblast light chains FLC1 and FLC2 synthesized in non-myogenic cells and presumptive myoblasts. There are immunological differences between the myosin heavy chains synthesized in myoblasts and myotubes and those synthesized in non-myogenic cells and presumptive myoblasts. Fluorescein-labeled antibodies against skeletal light meromyosin are bound only along the lateral edges of emerging and definitive A-bands. This antibody to light meromyosin is not bound to the outside of, or the microfilaments subtending, the plasma membrane in non-myogenic cells or in myoblasts or in myotubes. These findings suggest that: (1) non-myogenic cells and replicating presumptive myoblasts synthesize similar myosin heavy and light chains; (2) replicating presumptive myoblasts synthesize a different set of myosins from those synthesized by their postmitotic daughters, the myoblasts; (3) the myosins associated with the plasma membranes of non-myogenic and myogenic cells are products of structural genes distinct from those coding for the myosins for skeletal myofibrils.