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1. In vitro selection for catalytic activity with ribosome display

Author

Amstutz, Patrick, Felletier, Joelle N., Guggisberg, Armin, Jermutus, Lutz, Cesaro-Tadic Sandro, Pluckthun, Andreas, and Zahnd, Christian

Subjects
Catalysts -- Usage, Ribosomes -- Research, Enzymatic analysis, Chemistry
Abstract

The first in vitro selection for catalyst in activity based on catalytic turnover by using ribosome display, a method, which does not involve living cells at any step, is discussed. The capacity to select directly for enzymatic activity using an entirely in vitro process may allow for a significant increase in the explorable sequence space relative to existing strategies.

Published
2002

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