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Background: The wild population of Hypoxis hemerocallidea continues to decline rapidly because of overharvesting for medicinal use. This has necessitated the development of sustainable cultivation protocols for the species to promote its conservation. Aim: The impact of varying concentrations of compost tea extract on the growth, nutrient, antioxidant, and phytochemical contents of slow-growing corms H. hemerocallidea were investigated. Setting: Corms of H. hemerocallidea were irrigated with municipal water and graded concentrations (0.25, 0.5, 0.75 and 1) of compost tea. Methods: The nutrient content of the treated plant materials was analysed using the atomic absorption spectrophotometer while phytochemical and antioxidant contents were analysed following referenced methods. Results: The highest growth parameters were recorded in corms treated with 0.5 of compost tea. The extracted compost tea did not have a significant influence on the phenolic content and antioxidant capacity of the plant; however, significant variability was observed in the flavonols and FRAP (ferric reducing antioxidant power) values at P 0.05. Similarly, the concentration of certain mineral elements such as N, P, K, Ca and Mg varied significantly in the leaves whereas elemental compositions of the treated roots of H. hemerocallidea. Conclusion: The compost tea did not have a significant effect on the phenolic content, oxygen radical absorbance capacity and ABTS (2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic) acid) antioxidant properties and nutrients in the plant although significant differences were observed in the flavonols and FRAP content of leaves, corms and roots. Contribution: The study contributes to the development of an organic cultivation protocol to conserve wild relatives of H. hemerocallidea.

The submontane forests in the Congo Basin have not been studied sufficiently in terms of floristic diversity, biomass, and geographic distribution. The region's lack of resources and rugged terrain are among the barriers to sampling biodiversity. This study focuses on two understudied submontane forest areas, Bakossi National Park (BNP) and Mt. Nlonako (MN), which form part of the continental Cameroon Mountains and are believed to have high species diversity and endemism. However, significant gaps exist in biodiversity data across the continental Cameroon Mountains. This study is the first detailed quantitative survey of forests in the BNP and MN through permanent plot sampling. The results of this study can be used to guide policies for managing montane ecosystems in Cameroon and enhancing conservation efforts. The study also provides an opportunity for long-term monitoring of forest health and REDD+ status.

Several mining-related pollutions, industrial waste, and soil deterioration define South Africa’s environmental landscape. These have led to the consumption of unhealthy food, contaminated agricultural products, and polluted water. The polluted environment has been linked to numerous diseases among the populace, thus making environmental remediation an important issue in South Africa. Phytoremediation has been identified as a biological method for the restoration of polluted environments naturally and holistically. Therefore, it is vital to evaluate the level of phytoremediation-related research in South Africa in pursuit of a way out of environmental pollution. Thus, the purpose of this study was to map phytoremediation-related research in South Africa from inception to 2022. Statistical records from the Web of Science Core Collection were analyzed with the bibliometric package in RStudio, while mapping was performed via VOSviewer. Our study showed a low annual growth rate of publication (4.49%). The analysis uncovered that the 39 documents analyzed were written by 112 authors, and the first document was featured in the Journal of Geochemical Exploration in 1997. Kirkham, MB and Liphadzi, MS are the most relevant authors. USA has the strongest collaboration with South Africa, while the International Journal of Phytoremediation, the South African Journal of Botany, and Water SA are the most relevant journals. The result of this study can guide upcoming researchers and policymakers, together with essential facts for enhancing the restoration of the polluted environment in the country.

Background: Aquaponics and hydroponics are potential alternative techniques for sustainable cultivating of medicinal plants. Setting: The experiments were carried out on the Bellville campus of the Cape Peninsula University of Technology, Cape Town, South Africa. Aim: The aim of this study was to evaluate crop yield, secondary metabolite contents, and the antifungal activities of extracts from Helichrysum cymosum (H. cymosum) grown in aquaponic and hydroponic systems compared with field-collected plants. Methods: Helichrysum cymosum seedlings were cultivated in hydroponic and aquaponic systems for 6 weeks under greenhouse conditions. The data on plant growth parameters, phytochemical analyses of the leaves, anti-Fusarium oxysporum (F. oxysporum) activity of ethanolic extracts, and antioxidant capacities were recorded. Results: The results showed that the heights of plants grown in aquaponics and hydroponics did not differ substantially (p 0.05). The total polyphenol contents varied significantly (p 0.01) among the three cultivation techniques, with the field-collected plants yielding the highest contents (452.10 mg GAE/g ± 53.37 mg GAE/g). The flavonol contents differed significantly among the three cultivation techniques (p 0.05), with the highest flavonol contents in the field-collected plants (250.62 mg QE/g ± 58.12 mg QE/g). The plants grown in aquaponics had the highest number of compounds (104). The microdilution bioassay showed that the ethanolic extracts of field-harvested H. cymosum had higher fungistatic activity against F. oxysporum. The highest antioxidant capacity was recorded in 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay for plants cultivated in hydroponics (p 0.05), while field-collected plants showed a significantly higher value of 2719.42 µmol ± 278.72 µmol AAE/g (p 0.05) in the ferric ion reducing antioxidant parameter (FRAP) assay. Conclusion: The field-collected plants performed better in phytochemical contents. However, cultivation of H. cymosum using a hydroponic system may be feasible based on the antioxidant results. Contributions: The study contributes to developing an alternative strategy for cultivating plants and promoting sustainable farming.

Synthetic pesticides have been and are still the mainstay of pest management [...]

The chemical analysis of vineyards is an essential tool for the early detection of risks, such as excessive fertilization and heavy metal and pesticide contamination in farm management. Soil and plant samples were collected in summer and winter from six different vineyards with varying agricultural practices in the Cape Winelands of the Western Cape Province, South Africa. The samples were pretreated in a microwave using CEM MARS 6™ Microwave Digestion and Extraction System (CEM Corporation, Matthews, NC, USA). Chemical element data were obtained using an inductively coupled plasma optical emission spectrometer (ICP-OES) (ICP Expert II, Agilent Technologies 720 ICP-OES). The data will be valuable for selecting and improving farming practices and gaining insights into the influence of seasonal variation and agricultural practices on the elemental accumulation in farmlands.

Abstract Fungal invasions only recently started to receive more attention in invasion biology. This is largely attributed to little or non‐existent information about these inconspicuous organisms. Most invasion hypotheses focus on factors that increase invasion success; few try to explain why invasions fail. Here we hypothesize that a host–pathogen relationships can limit the invasiveness of an alien plant species in a novel range. To test this, we investigate whether the invasiveness of the Australian genus of Proteaceae, Banksia, in South Africa is determined by the alien and major invasive phytopathogen, Phytophthora cinnamomi. The presence of P. cinnamomi in Banksia root and soil was evaluated using morphological and molecular techniques. Isolates were cultured onto selective media and polymerize chain reactions and internal transcribing spacers were used for identification. Acetone leaf extracts of 11 Banksia spp. were screened for antimicrobial activity against P. cinnamomi, using the minimum inhibitory concentration assay. A total of 3840 Banksia individuals from seven localities were surveyed. Phytophthora cinnamomi was consistently isolated from Banksia species root and soil samples. Out of the 12 Banksia species that were screened for antimicrobial activity, four introduced species, B. burdettii, B. coccinea, Banksia hookeriana, and B. prionotes and the invasive B. integrifolia and B. ericifolia exhibited relatively high antimicrobial activity against P. cinnamomi (strain 696/12). We show that the phytopathogen in the native range has similar impact in the novel range and in doing so may limit invasion success of Banksia species with low antimicrobial activity.

Background: Allium species are generally reputed for their anti-pest properties; however, few studies have focused on optimising the quality and yield of anti-pest bioactive materials from these medicinal plants. Setting: Laboratory and greenhouse experiments were carried out on the Bellville campus of the Cape Peninsula University of Technology, Cape Town. Aims: This research study aimed to evaluate the effect of light intensity on the volatile constituents, antifungal and anti-insect activities of extracts obtained from Allium porrum L. cultivated hydroponically under greenhouse conditions. Methods: Seedlings of A. porrum were hydroponically grown under 40% shading and 0% shading conditions for 12 weeks. The phytochemical constituents of the aerial parts (leaf and bulb) of A. porrum were analysed. The antifungal activity against Fusarium oxysporum and the anti-insect activity on the grapevine mealybug (Planococcus ficus) was evaluated by microdilution and repellency bioassays, respectively. Results: Remarkably, the total polyphenol content was statistically higher (DF = 1, 6; F = 9.17; p 0.05) in plants exposed to 40% shade treatment. The gas chromatography-mass spectrometry (GC-MS) analysis revealed that the volatile compounds varied significantly (DF = 1; χ 2 = 3.435; p 0.05) between the two treatments. However, although a higher number of compounds (73) occurred in plants exposed to 40% shade than in those exposed to 0% shade (58), the shading effect on the number of compounds was not significant (DF = 1; χ 2 = 69.551; p 0.05). The acetone extracts of A. porrum that were cultivated under lower light irradiance showed a higher fungistatic activity against F. oxysporum in the antifungal bioassay. Conclusion: Broadly, this study revealed that lowering light intensity from 313 μmol m-2 s-1 to 153 μmol m-2 s-1 favoured a higher phenolic content, volatile constituents and higher anti-F. oxysporum activities in leeks.

A workshop on ticks and tick-borne diseases (T&TBDs) was held on June 25 and 26, 2019, at the Tropical Pesticides Research Institute, Division of Livestock and Human Diseases Vector Control, Arusha, Tanzania. The objectives of the workshop were to discuss the current situation and to formulate actionable strategies to improve surveillance and control of T&TBDs in Africa. The workshop was funded by the National Research Foundation and the Cape Peninsula University of Technology and attended by livestock health providers, farmers, and researchers from East, West, and Southern African countries. During the workshop, experts presented recent surveillance data focused on T&TBDs; participants discussed research opportunities and community engagement. The primary outcome of the workshop was the creation of a new research consortium known as The African Consortium for T&TBDs. The consortium is intended to function as a community for researchers, students, farmers, policymakers, extension workers, and community members who are interested in the advancement of T&TBD control. The consortium will engage in research activities that focus on comprehensive surveillance of T&TBDs, developing tick acaricide resistance, alternative tick control programs, and policy development and education. These areas were identified as top priorities to be developed to improve T&TBD control on the continent.

Aim: The purpose of the present study was to evaluate the effects of Beauveria bassiana (Hypocreales) inoculum on plant growth, volatile constituents, and tick repellency of the extracts of Tulbaghia violacea (Amaryllidaceae). Materials and Methods: Eight-week-old potted seedlings of T. violacea were each inoculated with conidia of B. bassiana (strain SM3) suspended at a concentration of 1×106 conidia mL–1. Tissue colonization by fungal conidia was assessed after 3 weeks. Plant growth, volatile constituents, and tick repellency were assessed after 12 weeks post-treatment. Results: B. bassiana conidia successfully colonized leaf and root tissues of T. violacea. The growth of fungal hyphae out of the leaf and root sections occurred in 75% and 91.6% of plants, respectively. Inoculation of the plants with B. bassiana significantly (p

The wild population of Siphonochilus aethiopicus (Zingiberaceae) is being eroded due to several pharmacological benefits and the hidden economy credited to its ethnobotanical uses in Southern Africa. This has called for the adoption of sustainable ways of cultivating the species without compromising its bioactive constituents. In this study, compost tea was brewed and applied at different dosages to potted S. aethiopicus to enhance its growth quality, phytochemical content, and elemental compositions. Treatments comprised 0.25, 0.50, 0.75 and 1.00 (vv) graded concentrations of compost tea, while water and undiluted compost tea were the control treatments. Results showed that dosages of compost tea had no significant effect on chlorophyl content or fresh and dry weights of rhizomes of S. aethiopicus. The longest leaves were recorded in plants irrigated with water only, while the shortest leaves were recorded in plants irrigated by 50% compost tea. A similar trend was observed in leaf width, except that equivalent values were recorded in all compost tea treatments while plants irrigated with undiluted compost tea were tallest. The highest and lowest flavanols were respectively recorded in 0.50 and 0.25 compost tea-treated plant samples, while undiluted compost tea yielded the highest flavonol and phenolic acids. The highest antioxidant contents were produced by the 0.25 compost tea-treated samples in the ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)), FRAP (Ferric Reducing Antioxidant Power) and ORAC (Oxygen Radical Antioxidant Capacity) assays, while the lowest were observed in plants irrigated with water only, although all compost tea-treated plants had equivalent effects on the ORAC content. The highest N, P, K, and Mn contents were produced in the 0.25-treated samples, while the minerals were least accumulated in samples treated with water only. All treatments had equivalent effects on Ca, Zn, and B yield, whereas the highest and equivalent accumulations of Mg and Na were recorded in the control treatments. Iron (Fe) and Cu were most influenced significantly by water whereas P, Ca and Zn tissue concentration was not significantly influenced by treatments. These results indicate that compost tea can optimize growth, mineral accumulation, phytochemicals, and antioxidants in S. aethiopicus. This approach serves as a greener and sustainable way of conserving overexploited indigenous medicinal plants such as S. aethiopicus to mitigate overexploitation of its wild relatives and preserve its genome from imminent extinction.

Heavy metal toxicity is a major threat to the health of both humans and ecosystems. Toxic levels of heavy metals in food crops, such as grapes, can have devastating effects on plant health and the market value of the produce. Two important factors that may influence the prevalence of heavy metals in grapevines are seasonal change and farming practices. The objectives of this study were (i) to conduct a detailed pioneer screening of heavy metal levels in soils and grapevine leaf tissues in selected wine farms and (ii) to study the influence of season and farming on heavy metal levels in soils and grapevine leaf tissues. Soil and grapevine leaf samples were collected from demarcated areas in selected vineyards in the Cape Winelands region of South Africa. The sampling was conducted in winter and summer from the same sites. The soil and leaf samples were analysed using inductively coupled plasma mass spectrometry (ICP-MS) techniques. The pooled data from the farms practising conventional or organic farming showed that seasonal variation had no significant effect (DF = 1, 22; p > 0.05) on the heavy metal contents in the soil. When the soil data from the winter and summer months were compared separately or pooled, the influence of agricultural practice was well-pronounced in As (DF = 1, 22, or 46; p < 0.05) and Cu (DF = 1, 22, or 46; p p< 0.05) Cu, As, Cr, and Hg uptake, with little effect on Ni, Co, Cd, and Hg leaf contents. Generally, the heavy metals studied (Cr, Co, Ni, Zn, As, Cd, Hg, and Pb) were substantially below the maximum permitted levels in plant and soil samples, per the recommendations of the WHO and Er indices, respectively. However, moderate contamination of the soils was recorded for Cr, Ni, Zn, and Pb. Remarkably, the Cu levels in the organic vineyard soils were significantly higher than in the conventional vineyards. Furthermore, based on the Igeo index, Cu occurred at moderate to heavy contamination levels.

Background: The increasing prevalence of cancer is placing enormous pressure on health infrastructure globally. The ever rising cancer burden is not unique to South Africa but also to many low- and middle-income countries. Natural plant-based products have for long have been used traditionally for treating cancer. Approximately 7% – 48% of cancer diagnosed patients take herbal medicines post diagnosis. As herbal remedies are also used by South Africans, it is justifiable to investigate herbal medicinal use in the prompt detection as well as prevention of cancer. Aim: The aim of this article is to highlight the potential of South African medicinal plants to combat cancer. Method: This review summarises previous research (1991–2020) on the impact of traditional herbal medicine in managing cancer, and identifies the context between traditional and conventional medicines. Scientific databases such as Science Direct, PubMed, Research Gate, and Google Scholar were used to source primary and secondary data for this review. Results: The findings of the present study call for the integration of herbal medicines into the existing healthcare systems to encourage the open use of herbal medicines by cancer patients. In addition, this study revealed 19 medicinal plant species from 15 families that are commonly used for the management of cancer in South Africa’s nine provinces. Conclusion: It is crucial to enhance collaboration between the existing healthcare systems and herbal traditional medicines in the provision of better care to patients at risk of, or who have been diagnosed with, cancer.

Background: Salvia dolomitica Codd. and Salvia namaensis Schinz. are indigenous to southern Africa and are used as medicinal plants in folk medicine. Aim: This study aimed to assess the effects of different levels of water deficit treatments on the growth, concentration of secondary metabolites, and anti-Fusarium oxysporum activity of S. dolomitica and S. namaensis. Setting: Experiments were carried out on the Bellville campus of the Cape Peninsula University of Technology, Cape Town. Methods: Four weeks old seedlings of the two species were subjected to 3-, 6-, 9- and 12-day water deficit regimes. Secondary metabolites such as polyphenols, alkaloids, and flavanols were assessed using spectroscopic methods. The anti-fungal activities of crude extracts obtained from plants were evaluated in a micro-dilution bioassay. Results: In all treatments, the plant height, crown width, number of stems and leaves, and fresh and dry weights reduced with increased water deficits. Acetone extracts from all treatments showed anti-fungal activity. However, extracts from the treatment with moderate water deficit (6-day watering interval) recorded significantly (P 0.01) better inhibition of F. oxysporum at the 18 h post incubation than the commercial fungicide, Mancozeb. Conclusion: This research has revealed that mild to moderate water deficit level favours the accumulation of alkaloids in S. dolomitica. Meanwhile, mild to severe water deficit significantly lowered flavanol content in S. namaensis. There was a correlation between the increase in total alkaloid contents and the enhanced anti-fungal activity of extracts of S. dolomitica. The present findings pave the way for optimised cultivation of medicinal plants and development of bioactive natural products.

Studying the mechanisms through which endophytic fungi confer protection to host plants against parasites will contribute toward elucidating the endophytic fungi–plant–pathogen relationship. In this study, we evaluated the effects of endophytic Beauveria bassiana on the antioxidant activity, oxidative stress, and growth of tomatoes infected with the fusarium wilt pathogen, Fusarium oxysporum f. sp. lycopersici (FOL). Tomato seedlings were inoculated with B. bassiana conidia and then contaminated with FOL experimentally. Four treatments (Control [T1], FOL only [T2], B. bassiana only [T3], and B. bassiana and FOL [T4]) were assessed. The plants from the B. bassiana and FOL treatment (T4) were significantly taller (DF = 3, 56; p < 0.001) and produced more leaves and aerial part biomass than those treated with only FOL (T2). Remarkably, plants in the two treatments with FOL (T2 and T4) had the lowest antioxidant activities; meanwhile, plants from the FOL treatment (T2) had the lowest ROS (superoxide and hydroxyl radicals) contents. Broadly, strong positive correlations between ROS and all the plant growth parameters were recorded in this study. While the current results revealed that the endophytic entomopathogen B. bassiana enhanced antioxidant capacity in plants, it did not improve the antioxidant capacity of F. oxysporum-infected plants. It is possible that the pathogenic FOL employed a hiding strategy to evade the host immune response and the antagonistic actions of endophytic B. bassiana. In conclusion, B. bassiana inoculum enhanced the growth of tomatoes infected with FOL, induced higher oxidative stress in both F. oxysporum-infected and -uninfected tomatoes, and improved antioxidant activities in plants inoculated with B. bassiana only.

The overexploitation of wild plants for medicinal uses and conventional agricultural farming methods, which use high amounts of water, fertilizers, and pesticides, have had devastating environmental consequences. This study aimed to evaluate the prospects of using aquaponics and hydroponics as alternative approaches to soil cultivation by comparing the crop yields, secondary metabolite contents, and the antifungal activities of ethanol extracts of Helichrysum odoratissimum (L.) Sweet, a widely used medicinal plant species in Southern Africa. Six-week-old H. odoratissimum seedlings were grown in aquaponic and hydroponic systems. The growth parameters, secondary metabolite contents, and antifungal activity against Fusarium oxysporum were assessed. The results for crop yield (plant height, fresh and dry weights) and the tissue nutrient contents did not change substantially (p > 0.05) between aquaponic and hydroponic treatments. Gas chromatography–mass spectrometry (GC–MS) analysis showed that monoterpenes and sesquiterpenes were the most abundant compounds in H. odoratissimum; however, no statistical difference was observed among the field, hydroponic, and aquaponic plants (DF = 2; χ2 = 2.67; p > 0.05). While there was no significant difference in polyphenol contents among the three treatments, remarkably, the flavonol contents in the leaves varied significantly (DF = 2; χ2 = 6.23; p < 0.05) among the three treatments. A higher flavonol content occurred in leaves from the hydroponic system than in leaves from the aquaponic (p < 0.05) and field (p > 0.05) systems. The MIC results showed that the ethanolic extract of H. odoratissimum was fungistatic against F. oxysporum; however, this effect was more prominent in the ethanol extracts of plants grown in the aquaponic system, with a mean MIC value of 0.37 ± 0.00 mg/mL The key findings of this study are that aquaponically cultivated plants exhibited the best antifungal activity, while higher total flavonol contents occurred in the hydroponically cultivated plants. In conclusion, aquaponics and hydroponics performed better or similar to field cultivation and are viable alternative methods for cultivating H. odoratissimum plants.

The main aim of this research study was to assess the interactive effects of water deficiency and the inoculation of a growth medium with Beauveria bassiana on plant growth, nutrient uptake, secondary metabolite contents, and antioxidant capacity of Allium cepa. A. cepa seedlings were simultaneously exposed to one of three watering regime treatments (3-day, 5-day, and 7-day watering intervals) and B. bassiana or no-fungus treatment. While the longest watering interval induced reduced plant growth, plants inoculated with B. bassiana had better results than those in the no-fungus treatment. Significant interactive effects (DF = 2.0; p < 0.05) between fungus and the watering regime on P, K, and Fe contents were observed. Remarkably, at the 7-day watering interval, the polyphenol content (64.0 mg GAE/L) was significantly higher in the plants treated with B. bassiana than in the no-fungus-treated plants. The watering interval significantly affected (DF = 2, 6; F = 7.4; p < 0.05) total flavonol contents among the fungus-treated plants. The interaction of the watering interval and B. bassiana inoculation (DF = 2.0; F = 3.8; p < 0.05) significantly influenced the flavonol content in the onion bulbs and the antioxidant activities of onion bulbs in the FRAP assay (DF = 2.0; F = 4.1; p < 0.05).

A consortium of microbial community was used for the treatment of acid mine drainage wastewater laden with sulphate and heavy metals. The wastewater was treated in an anaerobic continuously stirred tank bioreactor. The microbial community activity increased the pH from 5.6 to 6.5, and improved sulphate removal up to 85% from an initial sulphate concentration of 8080 mg SO42−/L in a continuous mode, following enrichment for 21 d. The maximum heavy metal removal percentage was observed for Cd (98%), Al (97%), Mn (95%), Pb (94%), Sr (94%) and Cu (91%). The microbial community showed synergy between strictly anaerobic and facultative Firmicutes sp., which were responsible for the bioreactor performance. The biochemical reaction indicated the microbial community has a wider range of substrates dominated by metallo-aminopeptidases.

Optimization of the quality and quantity of medicinal materials during cultivation could improve the value of medicinal plants. Light intensity and water availability affect physiological processes and growth of plants. Tulbaghia violacea L. (Alliaceae) bulbs and leaves are widely used traditionally in southern Africa for treatments of many ailments. The interactive effects of light intensity and watering regime on plant growth, nutrient uptake and antifungal activity of T. violacea were evaluated in the current study. Seedlings of T. violacea were grown hydroponically under two shading levels (0% and 40%) while being exposed simultaneously to one of three watering intervals: 5-day, 14-day and 21-day. Different growth parameters (plant height, plant fresh and dry weights) and tissue nutrient contents were evaluated at the end of the experiment. The antifungal activity of acetone extracts on Fusarium oxysporum were evaluated in a microdilution bioassay. Generally, significantly higher concentrations of macronutrients were recorded in the tissue of plants exposed to shorter watering interval. The results showed that different watering frequencies and light intensities significantly (p < 0.05) influenced plant growth parameters (height, and dry and fresh weights). Moreover, there were strong interactive effects of watering frequency and light intensity on most of the plant growth parameters. Remarkably, plants that were simultaneously exposed to the extended watering interval (21-day) and low light intensity showed the best anti-F. oxysporum activity. Key findings of this study revealed that shading alleviated the negative effects of water-deficit stress on plant growth and improved antifungal activity.

Research highlights: This study is one of few detailed analyses of plant diversity and vegetation patterns in African dry forests. We established permanent plots to characterize plant diversity, above-ground biomass, and vegetation patterns in a tropical dry forest in Kimbi-Fungom National Park, Cameroon. Our results contribute to long-term monitoring, predictions, and management of dry forest ecosystems, which are often vulnerable to anthropogenic pressures. Background and objectives: Considerable consensus exists regarding the importance of dry forests in species diversity and carbon storage; however, the relationship between dry forest tree species composition, species richness, and carbon stock is not well established. Also, simple baseline data on plant diversity are scarce for many dry forest ecosystems. This study seeks to characterize floristic diversity, vegetation patterns, and tree diversity in permanent plots in a tropical dry forest in Northwestern Cameroon (Kimbi-Fungom National Park) for the first time. Materials and methods: We studied associations between above-ground biomass and species composition, and how different vegetation types vary in terms of species composition, diversity, and carbon storage, in a dry forest in Kimbi-Fungom National Park, Cameroon. Vegetation was inventoried in 17 permanent 1-ha plots. Allometric equations were used to calculate above-ground biomass and carbon. Results: We found an average of 269.8 tree stems ha−1 and 43.1 species ha−1. Five vegetation types: semi-deciduous, gallery, mixed vegetation, secondary and the grassland/woody savanna forest were classified using TWINSPAN analysis. The five vegetation types had an average above-ground biomass of 149.2 t ha−1 and 74.6 tC ha−1 of carbon in the 17 ha analyzed. Canonical correspondence analysis (CCA) showed the importance of semi-deciduous forest over grassland/woody savanna forest. Conclusions: This study demonstrated that the forest of the Kimbi-Fungom National Park is poor in plant diversity, biomass, and carbon, highlighting the need to implement efficient management practices. Fine-scale inventory data of species obtained in this study could be useful in developing predictive models for efficient management of tropical dry forests.

Shortage in insulin secretion or degradation of produced insulin is the principal characteristic of the metabolic disorder of diabetes mellitus (DM). However, because the current medications for the treatment of DM have many detrimental side effects, it is necessary to develop more effective antidiabetic drugs with minimal side effects. Alpha-glucosidase and alpha-amylase inhibitors are directly implicated in the delay of carbohydrate digestion. Pharmacologically, these inhibitors could be targeted for the reduction in glucose absorption rate and, subsequently, decreasing the postprandial rise in plasma glucose and the risk for long-term diabetes complications. The main objectives of this research study were to isolate different phytochemical constituents present in the methanolic extract of Plectranthusecklonii and evaluate their alpha-glucosidase and alpha-amylase inhibitory activities and antioxidant capacity. The phytochemical investigation of the methanolic extract of P. ecklonii yielded three known compounds, viz. parvifloron D, F, and G (1–3, respectively). Parvifloron G was isolated for the first time from P. ecklonii. The in vitro bio-evaluation of the methanolic extract of P. ecklonii and its isolated compounds against alpha-glucosidase showed that 3 exhibited moderate inhibitory activity with IC50 values of 41.3 ± 1.2 μg/mL. Molecular docking analysis confirmed the alpha-glucosidase inhibitory activity demonstrated by 3. Additionally, strong antioxidant capacities were demonstrated by 3 and 1 on ORAC (28726.1 ± 8.1; 3942.9.6.6 ± 0.1 µM TE/g), respectively, which were comparable with the reference antioxidant epigallocatechingallate (EGCG). Furthermore, 3 also showed strong activity on TEAC (3526.1 ± 0.6 µM TE/g), followed by 2 (1069.3 ± 2.4 µM TE/g), as well as on FRAP (1455.4 ± 2.0 µM AAE/g). The methanolic extract of P. ecklonii is a rich source of abietane diterpenes with strong antioxidant activities. This is the first scientific report on alpha-glucosidase and alpha-amylase inhibitory activities, molecular docking, and antioxidant capacities of P. ecklonii constituents.

Sustainability of nutrient requirements for microbial proliferation on a large scale is a challenge in bioremediation processes. This article presents data on biochemical properties of a free cyanide resistant and total nitrogen assimilating fungal isolate from the rhizosphere of Zea mays (maize) growing in soil contaminated with a cyanide-based pesticide. DNA extracted from this isolate were PCR amplified using universal primers; TEF1-α and ITS. The raw sequence files are available on the NCBI database. Characterisation using biochemical data was obtained using colorimetric reagents analysed with VITEK® 2 software version 7.01. The data will be informative in selection of biocatalyst for environmental engineering application.

The endophytic plant-fungi symbiotic relationship can be explored to improve cultivation of targeted medicinal plant species. The objective of this study was to assess the effects of the cultivation of chive (Allium schoenoprasum) in plant growth medium inoculated with the entomopathogenic fungus, Beauveria bassiana (Hypocreales). Twelve replicates of chive seedlings were exposed to B. bassiana inoculum formulated at concentrations of 0, 1 × 105, 1 × 104 and 1 × 103 conidia mL−1 in a completely randomized design. We assessed plant growth parameters, such as leaf number and plant height weekly and root length, leaf and root fresh and dry weights and secondary metabolites three weeks post-fungal inoculation. The fungus was re-isolated from some of the leaves and roots of the treated plants suggesting that the fungus successfully colonized the plant tissue. Generally, the results indicated that the fungal inoculation had minimal effect on most of the growth parameters assessed in relation to the control. Remarkably, plants exposed to the fungus recorded greater (p < 0.05) total alkaloid, ranging from 2.98 – 3.76 mg atropine equivalent (AE)/g dry weight (DW) compared to the control plants (1.96 mg AE/g DW) for the leaves. This study demonstrated that endophytic fungi could be used to improve the yield of active chemical constituents in cultivated medicinal plants.

Background: The Rumpi Hills Forest Reserve (RHFR) is a montane forest area in south-western Cameroon. Although RHFR is presumed to be rich in biodiversity and vegetation types, little information exists regarding its floristic composition and vegetation patterns. Objectives: Our goal was to characterise vegetation patterns in the reserve and to understand how elevation influences distributions and diversity of species. We aimed to provide a first detailed plant species inventory for this important forest area, as well as basic information on forest structure. Method: We characterised floristic composition and vegetation patterns of the reserve in 25 1-ha plots along an elevational gradient from 50 m to 1778 m. In each plot, trees and lianas of diameter at breast height (dbh) ≥ 10 cm were measured; shrubs 10 cm were measured in nested plots of 0.01 ha. Results: In all, 16 761 trees, shrubs and lianas with dbh ≥ 1 cm were censused, representing 71 families, 279 genera and 617 morphospecies. Floristic composition ranged from 94 to 132 species, with a mean of 117.5 species per hectare in lowland forest (50 m – 200 m) and 36–41 species, with a mean of 38.5 species per hectare in montane cloud forest (1600 m – 1778 m) near the summit of Mount Rata. Two-way indicator species analysis classified the 25 plots into six vegetation types corresponding to lowland evergreen rainforest, lowland evergreen rainforest on basalt rocks, middle-elevation evergreen forest, submontane forest, transitional submontane forest and montane cloud forest. In all, 0.006% of the reserve was included in our sample plots. Detrended correspondence analysis highlighted the importance of elevation in shaping vegetation patterns. Conclusion: The RHFR is composed of different vegetation types, which show impressive variation in terms of structure, species composition and diversity. The detailed, fine-scale inventory data obtained in this study could be useful in planning efficient management of this and other montane tropical forests.

Endophytic entomopathogens have growth promoting, nutrient fortifying, and anti-insect properties that could improve the yield and quality of lettuce (Lactuca sativa L.). Lactuca sativa is a vegetable crop with high demand; however, it is susceptible to aphid infestations. This study’s objectives were to assess the pathogenicity of Beauveria bassiana (strain: SM3) (Bals.) Vuil. (Hypocreales) against Myzus persicae Sulzer, tissue colonization of lettuce by conidia of B. bassiana, as well as the effects of fungal inoculation on growth, tissue nutrient content, and proximate composition of the lettuce plants. Furthermore, the involvement of tissue nutrients in mediating the influence of endophytic fungus on the plant traits was examined. Insects and plants were exposed to four fungal conidial concentrations: 0, 1 × 106, 1 × 107 and 1 × 108 conidia mL−1 in an anti-insect bioassay and a greenhouse experiment, respectively. The B. bassiana strain was pathogenic against M. persicae, inducing mean insect mortality of 78% at the highest concentration (1 × 108 conidia mL−1). The B. bassiana endophytically colonized up to 76% of plants exposed to 1 × 108 conidia mL−1. Crown size and plant height varied significantly among treatments. However, the plant fresh and dry weights and nutrient elements N, P, K, Ca, and Mg did not vary significantly among treatments. Among the plant macronutrients assessed, only tissue carbon content was significantly (p < 0.01) affected by conidial treatments. The tissue C and Cu contents significantly correlated with the antioxidant capacity of the lettuce plants. Most of the micronutrients, viz. Mn, Fe, Cu, and B were remarkably higher (p < 0.05) in the fungus-treated plants than in the control plants. The antioxidant capacity (FRAP and TEAC) of plant extracts varied significantly (p < 0.001) among treatments, with the highest conidial treatment yielding the most increased antioxidant activity. In conclusion, the B. bassiana strain was endophytic to lettuce, pathogenic against M. persicae, and induced increased micro-nutrient tissue contents and antioxidant activities. This study demonstrated that B. bassiana could be potentially used in the biofortification of nutritive and medicinal qualities of plants.

Globally, fungal inocula are being explored as agents for the optimization of composting processes. This research primarily evaluates the effects of inoculating organic vegetable heaps with the entomopathogenic fungus Clonostachys rosea f. catenula (Hypocreales) on the biophysicochemical properties of the end-product of composting. Six heaps of fresh cabbage (Brassica oleracea var. capitata) waste were inoculated with C. rosea f. catenula conidia and another six were not exposed to the fungus. The composted materials from the fungus- and control-treated heaps were subsequently used as a medium to cultivate tomatoes (Solanum lycopersicum). The biophysicochemical characteristics of the composted materials were also assessed after composting. In addition, the protective effect of the fungal inoculum against red spider mite (Tetranychus urticae) infestations in the tomatoes was evaluated through the determination of conidial colonization of the plant tissue and the number of plants infested by the insect. Furthermore, phytotoxicity tests were carried out post experiment. There were few significant variations (p < 0.05) in heap temperature or moisture level between treatments based on the weekly data. We found no significant differences in the levels of compost macronutrient and micronutrient constituents. Remarkably, the composted materials, when incorporated into a growth medium from fungus-treated heaps, induced a 100% endophytic tissue colonization in cultivated tomato plants. While fewer red spider mite infestations were observed in tomato plants grown in composted materials from fungus-treated heaps, the difference was not significant (χ2 = 0.96 and p = 0.32). The fungal treatment yielded composted materials that significantly (p < 0.05) enhanced tomato seed germination, and based on the phytotoxicity test, the composted samples from the heaps exposed to the C. rosea f. catenula inoculum were not toxic to tomato seeds and seedlings. In conclusion, this study showed that C. rosea f. catenula improved the quality of composted materials in terms of fungal endophytism and seed germination.

Diabetes is one of the most dangerous metabolic disorders, with high rates of mortality worldwide. Since ancient times, medicinal plants have been used in traditional medicine to treat many diseases, including diabetes and its related complications. Plants are widely accepted, affordable, and perceived to have minimal adverse side effects. The Lamiaceae family is a potential source of therapeutic agents for the management of metabolic disorders, including diabetes. Hence, this review paper summarizes the antidiabetic use of Lamiaceae species in folk medicine globally. Furthermore, we present the antidiabetic activities and phytochemical constituents of twenty-three (23) Lamiaceae species and the antidiabetic activity of some notable chemical constituents isolated from some of these Lamiaceae species.

The kinetics of free cyanide biodegradation were investigated under simulated winter (5 °C) and optimum conditions (22 °C and pH of 11) using a Fusarium oxysporum isolate grown on Beta vulgaris waste as a sole carbon source in the presence of heavy metals, i.e. As, Fe, Cu, Pb, and Zn. The highest free cyanide degradation efficiency was 77% and 51% at 22 °C and 5 °C respectively, in cultures containing free cyanide concentration of 100 mg F-CN/L. When compared with the simulated winter conditions (5 °C), the specific population growth rate increased 4-fold, 5-fold, and 6-fold in 100, 200 and 300 mg F-CN/L, respectively, for cultures incubated at 22 °C in comparison to cultures at 5 °C; an indication that the Fusarium oxysporum cyanide degrading isolate prefers a higher temperature for growth and cyanide biodegradation purposes. The estimated energy of activation for cellular respiration during cyanide degradation was 44.9, 54, and 63.5 kJ/mol for 100, 200, and 300 mg F-CN/L cultures, respectively, for the change in temperature from 5 to 22 °C.

Diabetes mellitus (DM) is one of the most dangerous metabolic diseases with a high rate of mortality worldwide. It is well known that insulin resistance and deficiency in insulin production from pancreatic β-cells are the main characteristics of DM. Due to the detrimental side effects of the current treatment, there is a considerable need to develop new effective antidiabetic drugs, especially alpha-glucosidase and alpha-amylase inhibitors with lesser adverse effects. These inhibitors are known to be directly involved in the delay of carbohydrate digestion, resulting in a reduction of glucose absorption rate and, consequently, reducing the postprandial rise of plasma glucose, which can reduce the risk of long-term diabetes complications. Furthermore, natural products are well-known sources for the discovery of new bioactive compounds that can serve as scaffolds for drug discovery, including that of new antidiabetic drugs. The phytochemical investigation of Salvia aurita collected from Hogobach Pass, Eastern Cape Province, South Africa (SA), yielded four known abietane diterpenes namely carnosol (1), rosmanol (2), 7-methoxyrosmanol (3), 12-methoxycarnosic acid (4), and one flavonoid named 4,7-dimethylapigenin (5). Structural characterization of these isolated compounds was conducted using 1 and 2D NMR, in comparison with reported spectroscopic data. These compounds are reported for the first time from S. aurita. The biological evaluation of the isolated compound against alpha-glucosidase exhibited strong inhibitory activities for 3 and 2 with the half maximal inhibitory concentration (IC50) values of 4.2 ± 0.7 and 16.4 ± 1.1 µg/mL respectively, while 4 and 1 demonstrated strong alpha-amylase inhibitory activity amongst the isolated compounds with IC50 values of 16.2 ± 0.3 and 19.8 ± 1.4 µg/mL. Molecular docking analysis confirms the strong inhibitory activity of 3 against alpha-glucosidase. Additionally, excellent antioxidant capacities were displayed by 2, 1, and 3, respectively, with oxygen radical absorbance capacity (ORAC) (25.79 ± 0.01; 23.96 ± 0.01; 23.94 ± 0.02) mM Trolox equivalent (TE)/g; 1 and 2 as ferric-ion reducing antioxidant power (FRAP) (3.92 ± 0.002; 1.52 ± 0.002) mM ascorbic acid equivalent (AAE)/g; 5 and 2 as Trolox equivalent absorbance capacity (TEAC) (3.19 ± 0.003; 2.06 ± 0.003) mM TE/g. The methanolic extract of S. aurita is a rich source of abietane diterpenes with excellent antioxidant and antidiabetic activities that can be useful to modulate oxidative stress and might possibly be excellent candidates for the management of diabetes. This is the first scientific report on the phytochemical isolation and biological evaluation of the alpha-glucosidase and alpha-amylase inhibitory activities of Salvia aurita.

Endophytic entomopathogenic fungi are being explored for the management of phytophagous insect pests. The effects of Beauveria bassiana (Hypocreales) inoculation of grape plants on the infestation level of P. ficus, tissue nutrient contents, and growth and volatile constituents of potted grape plants were assessed. Grapevine plants were individually inoculated with a suspension of 1 × 108 conidia mL−1 of B. bassiana by drenching before experimentally infesting each of them with thirty adult females of P. ficus. At four weeks post-treatment, the fungus was re-isolated from leaves of 50% of the fungus-exposed plants. However, no significant difference (p > 0.05) was observed in all the plant growth parameters measured in the fungus-treated and control plants. Plant tissue analysis revealed markedly higher contents of calcium (Ca) and magnesium (Mg) in the leaf tissue of plants exposed to the B. bassiana relative to the control. Gas chromatography mass spectrometry (GC-MS) analyses showed that a significantly (X2 = 5.1; p < 0.02) higher number of known anti-insect volatile compounds (nine) were present among fungus treated plants compared to the control plants (five). Naphthalene, which is toxic to insects and humans, was detected only in the volatiles of the fungus-exposed plants. B. bassiana did not have any significant effect on total polyphenol, alkaloid, and flavonoids. Overall, treatment with fungus did not inhibit the infestation by P. ficus. In conclusion, these findings shed light on some of the mechanisms involved in endophytic fungus-plant-insect interactions.

In this study we examined the anti-tick properties of the essential oil of Tagetes minuta L. (Asteraceae: Asterales) against Hyalomma rufipes ticks. We obtained the essential oil of T. minuta by hydro-distillation of a combination of fresh flowers, leaves and soft stems, and analysed these by using gas chromatography (GC) and gas chromatography-linked mass spectrometry (GC-MS). The oil had a high percentage of monoterpenes and the major compounds identified were cis-ocimene (28.5%), beta-ocimene (16.83%) and 3-methyl-2-(2-methyl-2-butenyl)-furan (11.94%). Hyalomma rufipes adults displayed a significant (P