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4 results on '"Feline SCC"'

1. Felis catus papillomavirus type 2 E6 oncogene enhances mitogen-activated protein kinases and Akt activation but not EGFR expression in an in vitro feline model of viral pathogenesis.

Author

Altamura, Gennaro, Corteggio, Annunziata, and Borzacchiello, Giuseppe

Subjects
*PAPILLOMAVIRUSES, *MITOGEN-activated protein kinases, *EPIDERMAL growth factor receptors, *SQUAMOUS cell carcinoma, *GENE expression in viruses
Abstract

A possible causative role of Felis catus papillomavirus type 2 (FcaPV2) in the development of feline oral and cutaneous squamous cell carcinomas (SCC) has been recently suggested by demonstrating viral gene expression in vivo and transforming properties by its putative oncogenes E6 and E7 in vitro. The activated molecules MEK (pMEK), ERK (pERK) and Akt (pAkt) are signaling transduction effectors regulating cell proliferation and inhibition of apoptosis, which are critical steps towards tumour formation. Here, we show by Western blotting (WB) that expression of FcaPV2 E6 in feline epithelial cells enhances pMEK, pERK and pAkt levels compared to control cells. Additionally, we demonstrated by real-time quantitative PCR on epidermal growth factor receptor (EGFR) transcripts and WB that activation of these signaling routes is independent from EGFR differential gene expression, total protein levels or phosphorylation, unlike in human papillomavirus associated tumours. This study contributes to define the molecular scenario underlying FcaPV2-triggered pathogenesis of feline SCC. [ABSTRACT FROM AUTHOR]

Published
2016
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2. Transforming properties of Felis catus papillomavirus type 2 E6 and E7 putative oncogenes in vitro and their transcriptional activity in feline squamous cell carcinoma in vivo.

Author

Altamura, Gennaro, Corteggio, Annunziata, Pacini, Laura, Conte, Andrea, Pierantoni, Giovanna Maria, Tommasino, Massimo, Accardi, Rosita, and Borzacchiello, Giuseppe

Subjects
*VIRUS diseases, *SQUAMOUS cell carcinoma, *PAPILLOMAVIRUSES, *ONCOGENES, *GENETIC transcription, *APOPTOSIS, *VIRUSES, *CAT diseases
Abstract

Felis catus papillomavirus type 2 (FcaPV2) DNA is found in feline cutaneous squamous cell carcinomas (SCCs); however, its biological properties are still uncharacterized. In this study, we successfully expressed FcaPV2 E6 and E7 putative oncogenes in feline epithelial cells and demonstrated that FcaPV2 E6 binds to p53, impairing its protein level. In addition, E6 and E7 inhibited ultraviolet B (UVB)-triggered accumulation of p53, p21 and pro-apoptotic markers such as Cleaved Caspase3, Bax and Bak, suggesting a synergistic action of the virus with UV exposure in tumour pathogenesis. Furthermore, FcaPV2 E7 bound to feline pRb and impaired pRb levels, resulting in upregulation of the downstream pro-proliferative genes Cyclin A and Cdc2. Importantly, we demonstrated mRNA expression of FcaPV2 E2, E6 and E7 in feline SCC samples, strengthening the hypothesis of a causative role in the development of feline SCC. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

3. Transforming properties of Felis catus papillomavirus type 2 E6 and E7 putative oncogenes in vitro and their transcriptional activity in feline squamous cell carcinoma in vivo

Author

Annunziata Corteggio, Giovanna Maria Pierantoni, Andrea Conte, Massimo Tommasino, Gennaro Altamura, Giuseppe Borzacchiello, Laura Pacini, Rosita Accardi, Altamura, Gennaro, Corteggio, Annunziata, Pacini, Laura, Conte, Andrea, Pierantoni, GIOVANNA MARIA, Tommasino, Massimo, Accardi, Rosita, and Borzacchiello, Giuseppe

Subjects
Gene Expression Regulation, Viral, Transcriptional Activation, 0301 basic medicine, Felis catus papillomaviru, 040301 veterinary sciences, Papillomavirus E7 Proteins, Veterinary medicine, Cell, Cyclin A, Oncogenic viruse, Virus, Cell Line, 0403 veterinary science, Mice, 03 medical and health sciences, Downregulation and upregulation, Virology, medicine, Animals, RNA, Messenger, Papillomaviridae, Gene, E7, E6, Cyclin-dependent kinase 1, biology, Papillomavirus Infections, Oncogene Proteins, Viral, Feline oncology, 04 agricultural and veterinary sciences, Molecular biology, Veterinary virology, In vitro, 030104 developmental biology, medicine.anatomical_structure, Carcinoma, Squamous Cell, Cats, biology.protein, Carrier Proteins, Feline SCC, Oncovirus, Protein Binding, Signal Transduction
Abstract

Felis catus papillomavirus type 2 (FcaPV2) DNA is found in feline cutaneous squamous cell carcinomas (SCCs); however, its biological properties are still uncharacterized. In this study, we successfully expressed FcaPV2 E6 and E7 putative oncogenes in feline epithelial cells and demonstrated that FcaPV2 E6 binds to p53, impairing its protein level. In addition, E6 and E7 inhibited ultraviolet B (UVB)-triggered accumulation of p53, p21 and pro-apoptotic markers such as Cleaved Caspase3, Bax and Bak, suggesting a synergistic action of the virus with UV exposure in tumour pathogenesis. Furthermore, FcaPV2 E7 bound to feline pRb and impaired pRb levels, resulting in upregulation of the downstream pro-proliferative genes Cyclin A and Cdc2. Importantly, we demonstrated mRNA expression of FcaPV2 E2, E6 and E7 in feline SCC samples, strengthening the hypothesis of a causative role in the development of feline SCC.

Published
2016

4. Felis catus papillomavirus type 2 E6 oncogene enhances mitogen-activated protein kinases and Akt activation but not EGFR expression in an in vitro feline model of viral pathogenesis

Author

Gennaro Altamura, Giuseppe Borzacchiello, Annunziata Corteggio, Altamura, Gennaro, Corteggio, Annunziata, and Borzacchiello, Giuseppe

Subjects
Gene Expression Regulation, Viral, 0301 basic medicine, MAPK/ERK pathway, 040301 veterinary sciences, EGFR, pAkt, Biology, Microbiology, Cell Line, 0403 veterinary science, 03 medical and health sciences, Gene expression, Animals, Humans, Epidermal growth factor receptor, Papillomaviridae, Protein kinase B, Mitogen-Activated Protein Kinase Kinases, General Veterinary, Oncogene, Kinase, Cell growth, Epithelial Cells, Oncogenes, 04 agricultural and veterinary sciences, General Medicine, Molecular biology, MAPK, ErbB Receptors, 030104 developmental biology, Carcinoma, Squamous Cell, Cats, biology.protein, Phosphorylation, Veterinary (all), FcaPV2, Proto-Oncogene Proteins c-akt, Feline SCC
Abstract

A possible causative role of Felis catus papillomavirus type 2 (FcaPV2) in the development of feline oral and cutaneous squamous cell carcinomas (SCC) has been recently suggested by demonstrating viral gene expression in vivo and transforming properties by its putative oncogenes E6 and E7 in vitro. The activated molecules MEK (pMEK), ERK (pERK) and Akt (pAkt) are signaling transduction effectors regulating cell proliferation and inhibition of apoptosis, which are critical steps towards tumour formation. Here, we show by Western blotting (WB) that expression of FcaPV2 E6 in feline epithelial cells enhances pMEK, pERK and pAkt levels compared to control cells. Additionally, we demonstrated by real-time quantitative PCR on epidermal growth factor receptor (EGFR) transcripts and WB that activation of these signaling routes is independent from EGFR differential gene expression, total protein levels or phosphorylation, unlike in human papillomavirus associated tumours. This study contributes to define the molecular scenario underlying FcaPV2-triggered pathogenesis of feline SCC.

Published
2016

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