11 results on '"Feinstein LM"'
Search Results
2. Building Fences.
- Author
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Feinstein, LM
- Subjects
- BUILDING Fences (Poem), FEINSTEIN, L. M.
- Abstract
Presents the poem "Building Fences," by LM Feinstein. First Line: This is Sandburg kissing Frost hard; Last Line: and onto my freshly-swept porch.
- Published
- 2008
3. Patterns in spatial distribution and root trait syndromes for ecto and arbuscular mycorrhizal temperate trees in a mixed broadleaf forest.
- Author
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Valverde-Barrantes OJ, Smemo KA, Feinstein LM, Kershner MW, and Blackwood CB
- Subjects
- Carbon, Forests, Humans, Plant Roots, Syndrome, Trees, Mycorrhizae
- Abstract
Functional differences between trees with arbuscular (AM) or ectomycorrhizal (ECM) partnerships influence important ecological processes including nutrient cycling, community assembly, and biomass allocation patterns. Although most broadleaf temperate forests show both mycorrhizal types, relatively few studies have addressed functional difference among coexisting mycorrhizal tree species. The maintenance of ECM associations usually requires higher C investment than AM, leading to (A) lower root biomass and (B) more conservative root trait syndromes in ECM tree species compared to AM species. Here we quantified the representation and trait syndromes of 14 canopy tree species associated with either AM or ECM fungi in a natural forest community. Our results showed that, whereas species root abundance was proportional to basal area, some ECM tree roots were largely under-represented (up to ~ 33%). Most of the under-representation was due to lower than expected root abundance of Quercus rubra and Fagus grandifolia. Functional root traits in tree species were similar, with the exception of higher tissue density in ECM species. Moreover, closely related AM and ECM exhibited similar traits, suggesting inherited trait syndrome from a common ancestor. Thus, we found little evidence of divergent functional root trait syndromes between mycorrhizal types. Cores dominated by ECM species influenced trait distribution at the community level, but not total biomass, suggesting that mycorrhizal affiliation may have a stronger effect on the spatial distribution of traits but not on biomass stocks. Our results present an important step toward relating belowground carbon dynamics to species traits, including mycorrhizal type, in broadleaf temperate forests.
- Published
- 2018
- Full Text
- View/download PDF
4. Bacterial and Fungal Community Composition and Functional Activity Associated with Lake Wetland Water Level Gradients.
- Author
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Ma Y, Li J, Wu J, Kong Z, Feinstein LM, Ding X, Ge G, and Wu L
- Subjects
- Bacteria isolation & purification, Carbon metabolism, Chemical Phenomena, China, DNA Fingerprinting, Fungi isolation & purification, Polymorphism, Restriction Fragment Length, Soil chemistry, Bacteria classification, Biota, Fungi classification, Lakes, Soil Microbiology, Wetlands
- Abstract
The water regime is often the primary force driving the evolution of freshwater lakes, but how soil microbes responded to this process is far from understood. This study sampled wetland soils from a shallow lake that experienced water regime changes, Poyang Lake of China, to explore the features of bacterial and fungal community in response to water level changes. The soil physicochemical properties, T-RFLP based community structures and soil activities (including basal respiration, microbial biomass and enzymes) were all determined. Soil microbial eco-function was captured by testing the carbon metabolism with Biolog-Ecoplate. The results showed remarkable influence of the water level gradients on the soil physicochemical properties, microbial community structures and soil activities. However, the carbon utilization profile exhibited weak connections with the environmental variables and microbial community structures (p > 0.05). The microbial activities were significantly correlated with both bacterial and fungal community structures. Our results also emphasized the ascendant role of the deterministic process in the assemblages of microbial community structures and functions in wetland. In conclusion, this study revealed the discrepancy between community structures and eco-functions in response to water level gradients, and a relatively stable eco-function helped to maintain the ecosystem function of wetland from a long-term perspective.
- Published
- 2018
- Full Text
- View/download PDF
5. Transient Ischemic Attack Caused by Contrast Echocardiography in a Patient with Platypnea-Orthodeoxia.
- Author
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Main ML, Feinstein SB, Feinstein LM, Grayburn PA, and Wilson SR
- Subjects
- Humans, Male, Dyspnea diagnostic imaging, Echocardiography adverse effects, Hypoxia diagnostic imaging, Ischemic Attack, Transient etiology
- Published
- 2016
- Full Text
- View/download PDF
6. Aggregated and complementary: symmetric proliferation, overyielding, and mass effects explain fine-root biomass in soil patches in a diverse temperate deciduous forest landscape.
- Author
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Valverde-Barrantes OJ, Smemo KA, Feinstein LM, Kershner MW, and Blackwood CB
- Subjects
- Biomass, Ecosystem, Ohio, Soil, Species Specificity, Forests, Plant Roots physiology, Trees physiology
- Abstract
Few studies describe root distributions at the species level in diverse forests, although belowground species interactions and traits are often assumed to affect fine-root biomass (FRB). We used molecular barcoding to study how FRB of trees relates to soil characteristics, species identity, root diversity, and root traits, and how these relationships are affected by proximity to ecotones in a temperate forest landscape. We found that soil patch root biomass increased in response to soil resources across all species, and there was little belowground vertical or horizontal spatial segregation among species. Root traits and species relative abundance did not explain significant variation in FRB after correcting for soil fertility. A positive relationship between phylogenetic diversity and FRB indicated significant belowground overyielding attributable to local root diversity. Finally, variation in FRB explained by soil fertility and diversity was reduced near ecotones, but only because of a reduction in biomass in periodically anoxic areas. These results suggest that symmetric responses to soil properties are coupled with complementary species traits and interactions to explain variation in FRB among soil patches. In addition, landscape-level dispersal among habitats and across ecotones helps explain variation in the strength of these relationships in complex landscapes., (© 2014 The Authors New Phytologist © 2014 New Phytologist Trust.)
- Published
- 2015
- Full Text
- View/download PDF
7. Safety of ultrasound contrast agents in patients with known or suspected cardiac shunts.
- Author
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Parker JM, Weller MW, Feinstein LM, Adams RJ, Main ML, Grayburn PA, Cosgrove DO, Goldberg BA, Darge K, Nihoyannopoulos P, Wilson S, Monaghan M, Piscaglia F, Fowlkes B, Mathias W, Moriyasu F, Chammas MC, Greenbaum L, and Feinstein SB
- Subjects
- Albumins adverse effects, Contraindications, Fluorocarbons adverse effects, Humans, Contrast Media adverse effects, Echocardiography methods, Heart Diseases diagnostic imaging
- Abstract
Contrast-enhanced ultrasound imaging is a radiation-free diagnostic tool that uses biocompatible ultrasound contrast agents (UCAs) to improve image clarity. UCAs, which do not contain dye, often salvage "technically difficult" ultrasound scans, increasing the accuracy and reliability of a front-line ultrasound diagnosis, reducing unnecessary downstream testing, lowering overall health care costs, changing therapy, and improving patient care. Two UCAs currently are approved and regulated by the US Food and Drug Administration. They have favorable safety profiles and risk/benefit ratios in adult and pediatric populations, including compromised patients with severe cardiovascular diseases. Nevertheless, these UCAs are contraindicated in patients with known or suspected right-to-left, bidirectional, or transient right-to-left cardiac shunts. These patients, who constitute 10% to 35% of the general population, typically receive no UCAs when they undergo echocardiography. If their echocardiographic images are suboptimal, they may receive inappropriate diagnosis and treatment, or they may be referred for additional diagnostic testing, including radiation-based procedures that increase their lifetime risk for cancer or procedures that use contrast agents containing dye, which may increase the risk for kidney damage. An exhaustive review of current peer-reviewed research demonstrated no scientific basis for the UCA contraindication in patients with known or suspected cardiac shunts. Initial safety concerns were based on limited rodent data and speculation related to macroaggregated albumin microspheres, a radioactive nuclear imaging agent with different physical and chemical properties and no relation to UCAs. Radioactive macroaggregated albumin is not contraindicated in adult or pediatric patients with cardiac shunts and is routinely used in these populations. In conclusion, the International Contrast Ultrasound Society Board recommends removal of the contraindication to further the public interest in safe, reliable, radiation-free diagnostic imaging options for patients with known or suspected cardiac shunts and to reduce their need for unnecessary downstream testing., (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
8. The spatial scaling of saprotrophic fungal beta diversity in decomposing leaves.
- Author
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Feinstein LM and Blackwood CB
- Subjects
- Ecology methods, Polymorphism, Restriction Fragment Length, Trees microbiology, Biodiversity, Fungi genetics, Plant Leaves microbiology, Soil Microbiology
- Abstract
Assembly of fungal communities remains poorly understood in part because of the daunting range of spatial scales that may be involved in this process. Here, we use individual leaves as a natural sampling unit, comprising spatially distinct habitat and/or resource patches with unique histories and suites of resources. Spatial patterns in fungal beta diversity were tested for consistency with the metacommunity paradigms of species sorting and neutral dynamics. Thirty senesced leaves were collected from the forest floor (O horizon) in replicate upland forest, riparian forest and vernal pool habitats. We quantified spatial distance between leaves, and fungal community composition was assayed by terminal restriction fragment length polymorphism. Significant distance-decay relationships were detected at all but one upland site. This is the first study where changes in fungal community composition were quantified across discrete adjacent habitat patches, providing evidence that fungal distance decay is operational at a scale of centimetres. Although leaves of differing lignin contents were sampled from each site, leaf type was not consistently important in explaining variation in fungal community composition. However, depth of a leaf within the forest floor significantly influenced community composition at five of six sites. Environmental heterogeneity associated with depth could include moisture gradients, relative influence of soil or spore colonization, and impact of forest floor biotic community (i.e. collembola and earthworms). Because the influence of spatial distance and depth on fungal community composition could not be disentangled, both species-sorting and neutral processes may be embedded within the distance-decay relationships that we found., (© 2013 Blackwell Publishing Ltd.)
- Published
- 2013
- Full Text
- View/download PDF
9. Taxa-area relationship and neutral dynamics influence the diversity of fungal communities on senesced tree leaves.
- Author
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Feinstein LM and Blackwood CB
- Subjects
- Base Sequence, Ecosystem, Fungi genetics, Genetic Variation, Molecular Sequence Data, Biodiversity, Fungi classification, Plant Leaves microbiology, Trees microbiology
- Abstract
This study utilized individual senesced sugar maple and beech leaves as natural sampling units within which to quantify saprotrophic fungal diversity. Quantifying communities in individual leaves allowed us to determine if fungi display a classic taxa-area relationship (species richness increasing with area). We found a significant taxa-area relationship for sugar maple leaves, but not beech leaves, consistent with Wright's species-energy theory. This suggests that energy availability as affected plant biochemistry is a key factor regulating the scaling relationships of fungal diversity. We also compared taxa rank abundance distributions to models associated with niche or neutral theories of community assembly, and tested the influence of leaf type as an environmental niche factor controlling fungal community composition. Among rank abundance distribution models, the zero-sum model derived from neutral theory showed the best fit to our data. Leaf type explained only 5% of the variability in community composition. Habitat (vernal pool, upland or riparian forest floor) and site of collection explained > 40%, but could be attributed to either niche or neutral processes. Hence, although niche dynamics may regulate fungal communities at the habitat scale, our evidence points towards neutral assembly of saprotrophic fungi on individual leaves, with energy availability constraining the taxa-area relationship., (© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.)
- Published
- 2012
- Full Text
- View/download PDF
10. Placing the effects of leaf litter diversity on saprotrophic microorganisms in the context of leaf type and habitat.
- Author
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Wu L, Feinstein LM, Valverde-Barrantes O, Kershner MW, Leff LG, and Blackwood CB
- Subjects
- Bacteria genetics, Biodegradation, Environmental, Biomass, Carbon analysis, DNA, Bacterial genetics, DNA, Fungal genetics, Fungi genetics, Nitrogen analysis, Polymorphism, Restriction Fragment Length, Bacteria growth & development, Ecosystem, Fungi growth & development, Plant Leaves microbiology, Soil Microbiology
- Abstract
Because of conflicting results in previous studies, it is unclear whether litter diversity has a predictable impact on microbial communities or ecosystem processes. We examined whether effects of litter diversity depend on factors that could confound comparisons among previous studies, including leaf type, habitat type, identity of other leaves in the mixture, and spatial covariance at two scales within habitats. We also examined how litter diversity affects the saprotrophic microbial community using terminal restriction fragment length polymorphism to profile bacterial and fungal community composition, direct microscopy to quantify bacterial biomass, and ergosterol extraction to quantify fungal biomass. We found that leaf mixture diversity was rarely significant as a main effect (only for fungal biomass), but was often significant as an interaction with leaf type (for ash-free dry mass recovered, carbon-to-nitrogen ratio, fungal biomass, and bacterial community composition). Leaf type and habitat were significant as main effects for all response variables. The majority of variance in leaf ash-free dry mass and C/N ratio was explained after accounting for treatment effects and spatial covariation at the meter (block) and centimeter (litterbag) scales. However, a substantial amount of variability in microbial communities was left unexplained and must be driven by factors at other spatial scales or more complex spatiotemporal dynamics. We conclude that litter diversity effects are primarily dependent on leaf type, rather than habitat type or identity of surrounding leaves, which can guide the search for mechanisms underlying effects of litter diversity on ecosystem processes.
- Published
- 2011
- Full Text
- View/download PDF
11. Assessment of bias associated with incomplete extraction of microbial DNA from soil.
- Author
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Feinstein LM, Sul WJ, and Blackwood CB
- Subjects
- DNA, Bacterial analysis, DNA, Fungal analysis, Ecosystem, Electrophoresis, Gel, Pulsed-Field, Genes, rRNA, Molecular Sequence Data, Polymerase Chain Reaction methods, Polymorphism, Restriction Fragment Length, Sequence Analysis, DNA methods, Bacteria classification, Bacteria genetics, Bacteria isolation & purification, Bias, DNA, Bacterial isolation & purification, DNA, Fungal isolation & purification, Fungi classification, Fungi genetics, Fungi isolation & purification, Soil analysis, Soil Microbiology
- Abstract
DNA extraction bias is a frequently cited but poorly understood limitation of molecular characterizations of environmental microbial communities. To assess the bias of a commonly used soil DNA extraction kit, we varied the cell lysis protocol and conducted multiple extractions on subsamples of clay, sand, and organic soils. DNA, as well as bacterial and fungal ribosomal gene copies as measured by quantitative PCR, continued to be isolated in successive extractions. When terminal restriction fragment length polymorphism was used, a significant shift in community composition due to extraction bias was detected for bacteria but not for fungi. Pyrosequencing indicated that the relative abundances of sequences from rarely cultivated groups such as Acidobacteria, Gemmatimonades, and Verrucomicrobia were higher in the first extraction than in the sixth but that the reverse was true for Proteobacteria and Actinobacteria. This suggests that the well-known phylum-level bacterial cultivation bias may be partially exaggerated by DNA extraction bias. We conclude that bias can be adequately reduced in many situations by pooling three successive extractions, and additional measures should be considered when divergent soil types are compared or when comprehensive community analysis is necessary.
- Published
- 2009
- Full Text
- View/download PDF
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