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3. Criblage toxicologique urinaire par une méthode automatisée immunoenzymatique multiparamétrique basée sur une technique de biopuce : comparaison à de l'immunochromatographie et de la chromatographie liquide couplée à de la spectrométrie de masse

Author

Metsu, David, Nouhaud, Malika, El-Balkhi, Souleiman, Lavit, Michel, Paillard, Hélène, Berthomès, Pierre, Martinez, Chantal, Gicquel-Bordelongue, Nathalie, Blonstein, Benjamin, Latier, Julien, Billoré, Sabine, Chassagne, Claire, Lanot, Thomas, and Février, Frédéric

Published
2022
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4. Molecular and Epidemiological Evidence for Spread of Multiresistant Methicillin-Susceptible Staphylococcus aureus Strains in Hospitals

Author

Donnio, Pierre-Yves, primary, Février, Frédéric, additional, Bifani, Pablo, additional, Dehem, Marie, additional, Kervégant, Christèle, additional, Wilhelm, Nathalie, additional, Gautier-Lerestif, Anne-Lise, additional, Lafforgue, Nathalie, additional, Cormier, Michel, additional, and Le Coustumier, Alain, additional

Published
2007
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5. Molecular and Epidemiological Evidence for Spread of Multiresistant Methicillin-Susceptible Staphylococcus aureusStrains in Hospitals

Author

Donnio, Pierre-Yves, Février, Frédéric, Bifani, Pablo, Dehem, Marie, Kervégant, Christèle, Wilhelm, Nathalie, Gautier-Lerestif, Anne-Lise, Lafforgue, Nathalie, Cormier, Michel, and Le Coustumier, Alain

Abstract

ABSTRACTThe excision of the staphylococcal chromosomal cassette mec(SCCmec) from methicillin-resistant Staphylococcus aureus(MRSA) strains results in methicillin-susceptible S. aureus(MSSA) strains. In order to determine the proportion and diversity of multidrug-resistant MSSA (MR-MSSA) strains derived from MRSA strains, 247 mecA-negative isolates recovered in 60 French hospitals between 2002 and 2004 were characterized. The spatypes of all strains were determined, and a subset of the strains (n= 30) was further genotyped by multilocus sequence typing. The IDI-MRSA assay was used to test the isolates for the presence of the SCCmecelement, which was detected in 68% of all isolates analyzed. Molecular analysis of the samples suggested that 92% of the MR-MSSA isolates were derived from MRSA clones of diverse genetic backgrounds, of which the clone of sequence type 8 and SCCmectype IVAaccounted for most of the samples. High variations in incidence data and differences in the molecular characteristics of the isolates from one hospital to another indicate that the emergence of MR-MSSA resulted from independent SCCmecexcisions from epidemic MRSA isolates, as well as the diffusion of methicillin-susceptible strains after the loss of SCCmec. MR-MSSA could constitute a useful model for the study of the respective genetic and environmental factors involved in the dissemination of S. aureusin hospitals.

Published
2007
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6. Urine toxicological screening method using an immunoenzymatic assay based on a biochip array technology: comparative study with immunochromatography and Liquid chromatography–mass spectrometry

Author

Metsu D, Nouhaud M, El-Balkhi S, Lavit M, Paillard H, Berthomès P, Martinez C, Gicquel-Bordelongue N, Blonstein B, Latier J, Billoré S, Chassagne C, Lanot T, and Février F

Subjects
Benzodiazepines, Chromatography, Affinity, Chromatography, Liquid methods, Humans, Mass Spectrometry methods, Methadone, Analgesics, Opioid, Opiate Alkaloids
Abstract

Multiparametric toxicology research is mainly based on immunochromatography [IC] and chromatography methods. A new automated method using an immunoenzymatic (IE) assay based on a biochip array technology combines short turning around time and analytical performances close to chromatography in terms of positivity cut-off. The aim of our study was to compare IE versus IC and chromatography methods using urines samples from clinical cases. Seventy-two samples were analyzed by IC (amphetamines, opiates, benzodiazepines, THC, methadone, cocaine), IE and chromatography (previous classes plus opioids and cathinone). Immunochromatography results were read by at least 7 operators to assess reading subjectivity. Immunoenzymatic, IC, and chromatrography results were compared with each other. Chromatographic quantification was analyzed to understand discrepancies. Significant discrepancies (29-64%) were observed between IC and IE for most of the drug families investigated except for benzodiazepines, methadone and opiates. These discrepancies were not identified between IE and chromatography, except for some substances (28% to 67% discrepancies for buprenorphine, tramadol and oxycodone, 100% for cathinone). In contrast to IC, the performance of IE approached those of chromatography, except for some substances for which cross-reactions must be investigated. Reading discrepancies were frequent with IC (33% of samples) and made robust result output challenging. In conclusion, the Multistat® is an interesting method for first-line toxicological screening for laboratories without chromatography method.

Published
2022
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