1. Prognostic value of Ki67 in localized prostate carcinoma: a multi-institutional study of >1000 prostatectomies.
- Author
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Tretiakova, MS, Wei, W, Boyer, HD, Newcomb, LF, Hawley, S, Auman, H, Vakar-Lopez, F, McKenney, JK, Fazli, L, Simko, J, Troyer, DA, Hurtado-Coll, A, Thompson, IM, Carroll, PR, Ellis, WJ, Gleave, ME, Nelson, PS, Lin, DW, True, LD, Feng, Z, and Brooks, JD
- Subjects
Humans ,Prostatic Neoplasms ,Recurrence ,Prostate-Specific Antigen ,Ki-67 Antigen ,Neoplasm Staging ,Prognosis ,Prostatectomy ,Tissue Array Analysis ,Proportional Hazards Models ,Cell Proliferation ,Male ,Kaplan-Meier Estimate ,Neoplasm Grading ,Cancer ,Urologic Diseases ,Prostate Cancer ,Patient Safety ,Detection ,screening and diagnosis ,4.2 Evaluation of markers and technologies ,Oncology and Carcinogenesis ,Urology & Nephrology - Abstract
BackgroundExpanding interest in and use of active surveillance for early state prostate cancer (PC) has increased need for prognostic biomarkers. Using a multi-institutional tissue microarray resource including over 1000 radical prostatectomy samples, we sought to correlate Ki67 expression captured by an automated image analysis system with clinicopathological features and validate its utility as a clinical grade test in predicting cancer-specific outcomes.MethodsAfter immunostaining, the Ki67 proliferation index (PI) of tumor areas of each core (three cancer cores/case) was analyzed using a nuclear quantification algorithm (Aperio). We assessed whether Ki67 PI was associated with clinicopathological factors and recurrence-free survival (RFS) including biochemical recurrence, metastasis or PC death (7-year median follow-up).ResultsIn 1004 PCs (∼4000 tissue cores) Ki67 PI showed significantly higher inter-tumor (0.68) than intra-tumor variation (0.39). Ki67 PI was associated with stage (P
- Published
- 2016