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Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2019-09-19T14:08:15Z No. of bitstreams: 1 2012 - Pedro Ivan Fazio Junior.pdf: 1016463 bytes, checksum: 14de6b5137318bd0cc0e573e49d00fcb (MD5) Made available in DSpace on 2019-09-19T14:08:15Z (GMT). No. of bitstreams: 1 2012 - Pedro Ivan Fazio Junior.pdf: 1016463 bytes, checksum: 14de6b5137318bd0cc0e573e49d00fcb (MD5) Previous issue date: 2012-02-23 Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPQ The objective of this study was to evaluate the efficacy of a collar impregnated with flumethrin (2.5%) and propoxur (10%) against adults of Ctenocephalides felis felis and Rhipicephalus sanguineus on Beagle dogs, and to evaluate possible changes in serum cholinesterase (butyrylcholinesterase) after the use of the collar. Twenty Beagle dogs were divided into two groups, on 10 animals impregnated collars were placed and the other 10 remained untreated (control group). Each dog was infested with 50 pairs of C. felis felis and 25 pairs of R. sanguineus weekly during the first month, and after the second month every two weeks until day +210. Forty-eight hours after infestation, live fleas and ticks were recovered and counted from the animals. For determination of serum cholinesterase, blood samples were collected on days -14, -7, +1, +5, +10, +30, +45, +60, +75, +90, +105, +120, +135, +150, +165, +180, +195 and +210. All animals were evaluated weekly for signs consistent with clinical intoxication. At the end of the trial, the collars were removed, and after seven days, new measurement of cholinesterase was done. . The efficacy for C. felis felis was over 80% for up to day +168 after treatment. The efficacy for R. sanguineus was over 80% up to day +182. It was found that the collar has not decreased significantly (p ? 0.05) serum butyrylcholinesterase in dogs by comparing the means before and after treatment, however, the serum cholinesterase activity was significantly increased (p ? 0.05) after removal of the collars. It was evident that the impregnated with propoxur and flumethrin has an efficacy superior to five months in the control of C. felis felis and R. sanguineus. The alteration in serum cholinesterase levels by propoxur was not relevant and no signs of toxicity were observed during the experimental period O objetivo deste trabalho foi avaliar a efic?cia de uma coleira impregnada com Flumetrina (2,5%) e Propoxur (10%) sobre adultos de Ctenocephalides felis felis e Rhipicephalus sanguineus em c?es Beagle com avalia??o de poss?veis altera??es da colinesterase s?rica (butirilcolinesterase) ap?s o uso da coleira. Para isso foram utilizados 20 c?es da ra?a Beagle, divididos em dois grupos, onde em 10 animais foram colocadas as coleiras impregnadas e os outros 10 sem nenhum tratamento (controle). Cada c?o foi infestado com 50 casais de C. felis felis e 25 casais de R. sanguineus semanalmente no primeiro m?s e a partir do segundo m?s quinzenalmente at? o dia +210. Quarenta e oito horas ap?s as infesta??es foram realizadas avalia??es com contagem de pulgas e carrapatos vivos recuperados nos animais. Para dosagem da colinesterase s?rica foram coletadas amostras de sangue dos animais nos dias -14, -7, +1, +5, +10, +30, +45, +60, +75, +90, +105, +120, +135, +150, +165, +180, +195 e +210. Todos os animais foram avaliados semanalmente em rela??o a sinais cl?nicos compat?veis com intoxica??o. Posteriormente ao t?rmino do experimento de efic?cia as coleiras foram removidas e ap?s sete dias foi realizada nova dosagem da colinesterase. A efic?cia para C. felis felis foi superior a 80% at? o dia +168 ap?s tratamento. A efic?cia para R. sanguineus foi superior a 80% at? o dia +182. Foi verificado que a coleira n?o diminuiu significativamente (p?0,05) os n?veis s?ricos de butirilcolinesterase nos c?es comparando-se as m?dias antes e ap?s o tratamento, no entanto, a atividade s?rica da colinesterase aumentou significativamente (p?0,05) ap?s a retirada da coleira. Evidenciou-se que a coleira a base de propoxur e flumetrina apresenta efic?cia superior a cinco meses sobre C. felis felis e R. sanguineus. A altera??o da colinesterase s?rica dos animais pelo propoxur n?o foi relevante e nenhum sinal de toxicidade foi observado durante o per?odo experimental

O objetivo do presente trabalho foi avaliar a influência de dietas merídicas compostas por sangue de diferentes espécies animais sobre a emergência de adultos da pulga Ctenocephalides felis felis. Foram utilizadas seis dietas artificiais contendo areia e sangue desidratado de cão, boi, coelho e galinha, acrescidas ou não com farelo de trigo. Foram realizadas seis repetições contendo dez ovos de C. f. felis para cada dieta. Após 25 dias de incubação, os ovos foram quantificados e avaliados quanto à emergência de adultos. O número de pulgas emergidas para dietas com sangue de boi, cão, coelho e galinha, com areia e farelo de trigo, foi de aproximadamente oito pulgas. Para as dietas contendo apenas sangue de boi ou de cão e areia, essa emergência foi de aproximadamente uma pulga para ambas. Conclui-se que a origem do sangue empregado na elaboração da dieta não interfere significativamente no percentual de emergência de adultos de C. f. felis. Portanto, pode-se optar pela espécie animal disponível para o preparo da dieta artificial, aliada à suplementação com farelo de trigo, para suprir uma eventual perda nutricional decorrente da desidratação do sangue. The objective of the present study was to evaluate the influence of meridic diets composed by blood from different animal species upon the adult emergence of Ctenocephalides felis felis fleas. Six artificial diets containing dried blood of cattle (standard), dogs, rabbit and chicken, combined with sand or sand/wheat bran were prepared. For each diet six samples containing ten C. f. felis eggs were evaluated. After 25 days of incubation, samples were assessed for adult emergence. The number of emerge fleas for diets composed by bovine, canine, rabbit and chicken combined with sand/wheat bran was aproximally eight fleas. Diets composed by bovine and canine combined with sand, this emergence was aproximally one flea, for both. It can be concluded that the blood origin do not alter significantly C. f. felis adult emergence rate. Therefore, blood from any available animal species can be used for artificial diet formulation, combined with wheat bran in order to supply any nutritional losses occurred during blood dehydration.