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6. Expression of yeast homolog of the mammal BCRP gene coding for riboflavin efflux protein activates vitamin B2 production in the flavinogenic yeast Candida famata.

Author

Tsyrulnyk, Andriy O., Andreieva, Yuliia A., Ruchala, Justyna, Fayura, Lyubov R., Dmytruk, Kostyantyn V., Fedorovych, Daria V., and Sibirny, Andriy A.

Abstract

Candida famata is a representative of a group of so‐called flavinogenic yeast species that overproduce riboflavin (vitamin B2) in response to iron limitation. Overproduced riboflavin accumulates in the cultural medium rather than in the cells suggesting existence of the special mechanisms involved in riboflavin excretion. The corresponding protein and gene have not been identified in yeasts. At the same time, the corresponding gene BCRP has been identified in mammal mammary glands. Several homologs of the mammal BCRP gene encoding putative riboflavin efflux protein (excretase) were identified in Debaryomyces hansenii. The closest homolog was expressed under the control of D. hanseniiTEF1 promoter in the riboflavin overproducing strain of C. famata. Resulted transformants overexpressed the corresponding gene and produced 1.4‐ to 1.8‐fold more riboflavin as compared with the parental strain. They also were characterized by overexpression of RIB1 and RIB6 genes of riboflavin synthesis and exhibited elevated specific activity of GTP‐cyclohydrolase II. Membrane localization of the riboflavin excretase was confirmed by fluorescent microscopy. [ABSTRACT FROM AUTHOR]

Published
2020
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9. Expression of yeast homolog of the mammal BCRP gene coding for riboflavin efflux protein activates vitamin B 2 production in the flavinogenic yeast Candida famata.

Author

Tsyrulnyk AO, Andreieva YA, Ruchala J, Fayura LR, Dmytruk KV, Fedorovych DV, and Sibirny AA

Subjects
Animals, Candida classification, Cloning, Molecular, DNA, Fungal genetics, Riboflavin biosynthesis, ATP Binding Cassette Transporter, Subfamily G, Member 2 genetics, Candida genetics, Fungal Proteins genetics, Mammals genetics, Riboflavin metabolism
Abstract

Candida famata is a representative of a group of so-called flavinogenic yeast species that overproduce riboflavin (vitamin B 2 ) in response to iron limitation. Overproduced riboflavin accumulates in the cultural medium rather than in the cells suggesting existence of the special mechanisms involved in riboflavin excretion. The corresponding protein and gene have not been identified in yeasts. At the same time, the corresponding gene BCRP has been identified in mammal mammary glands. Several homologs of the mammal BCRP gene encoding putative riboflavin efflux protein (excretase) were identified in Debaryomyces hansenii. The closest homolog was expressed under the control of D. hansenii TEF1 promoter in the riboflavin overproducing strain of C. famata. Resulted transformants overexpressed the corresponding gene and produced 1.4- to 1.8-fold more riboflavin as compared with the parental strain. They also were characterized by overexpression of RIB1 and RIB6 genes of riboflavin synthesis and exhibited elevated specific activity of GTP-cyclohydrolase II. Membrane localization of the riboflavin excretase was confirmed by fluorescent microscopy., (© 2020 John Wiley & Sons, Ltd.)

Published
2020
Full Text
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