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Copyright of Egyptian Journal of Agronomy is the property of Egyptian National Agricultural Library (ENAL) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Abstract Background Sertraline is a well known antidepressant drug which belongs to a class called selective serotonin reuptake inhibitor. Most published methods do not enable studying the stability of this drug in different stress conditions. Results Two new methods were developed for the determination of sertraline (SER). Both methods are based on coupling with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in borate buffer of pH 7.8 and measuring the reaction product spectrophotometrically at 395 nm (Method I) or spectrofluorimetrically at 530 nm upon excitation at 480 nm (Method II). The response-concentration plots were rectilinear over the range 2-24 μg/mL and 0.25-5 μg/mL for methods I and II respectively with LOD of 0.18 μg/mL and 0.07 μg/mL, and LOQ of 0.56 μg/mL and 0.21 μg/mL for methods I and II, respectively. Conclusion Both methods were applied to the analysis of commercial tablets and the results were in good agreement with those obtained using a reference method. The fluorimetric method was further applied to the in vivo determination of SER in human plasma. A proposal of the reaction pathway was presented. The spectrophotometric method was extended to stability study of SER. The drug was exposed to alkaline, acidic, oxidative and photolytic degradation according to ICH guidelines. Moreover, the method was utilized to investigate the kinetics of oxidative degradation of the drug. The apparent first order rate constant and t1/2 of the degradation reaction were determined.

Abstract Background Pregabalin (PG) is an anticonvulsant, analgesic and anxiolytic drug. A survey of the literature reveals that all the reported spectrophotometric methods are either don't offer high sensitivity, need tedious extraction procedures, recommend the measurement of absorbance in the near UV region where interference most probably occurs and/or use non specific reagent that don't offer suitable linearity range. Results Two new sensitive and simple spectrophotometric methods were developed for determination of pregabalin (PG) in capsules. Method (I) is based on the reaction of PG with 1,2-naphthoquinone-4-sulphonate sodium (NQS), yielding an orange colored product that was measured at 473 nm. Method (II) is based on the reaction of the drug with 2,4-dinitrofluorobenzene (DNFB) producing a yellow product measured at 373 nm. The different experimental parameters affecting the development and stability of the reaction product in methods (I) and (II) were carefully studied and optimized. The absorbance-concentration plots were rectilinear over the concentration ranges of 2-25 and 0.5-8 μg mL-1 for methods (I) and (II) respectively. The lower detection limits (LOD) were 0.15 and 0.13 μg mL-1 and the lower quantitation limits (LOQ) were 0.46 and 0.4 μg mL-1 for methods (I) and (II) respectively. Conclusion The developed methods were successfully applied to the analysis of the drug in its commercial capsules. The mean percentage recoveries of PG in its capsule were 99.11 ± 0.98 and 100.11 ± 1.2 (n = 3). Statistical analysis of the results revealed good agreement with those given by the comparison method. Proposals of the reaction pathways were postulated.

The article discusses the emergence of low back disorders (LBDs) continuously becoming a major occupational health issue facing the agricultural workforce. It is suggested that working in a flexed or stooped posture is one of several workplace factors associated with occupationally related LBDs and is highly prevalent in many agricultural works. According to the author, the examination of the epidemiological literature, scope of stooped work in agriculture and the current and potential intervention approaches will evaluate the role of stooped work in the LBDs.

The article evaluates the commonly used winegrape trellis systems with regard to the risk of developing musculoskeletal injuries to the wrist and lower back of a person while pruning in California. Eleven compensated workers at the vineyards administered by the Department of Viticulture and Enology participated in this study. The participants performed a simulated pruning task as wrist and trunk postures were gathered using electrogoniometers. The results showed postural differences among the trellis systems. Compared to the other systems, the vertical shoot positioned was determined to be the optimal system in terms of decreasing relative musculoskeletal disorder risk.

Most biomechanical assessments of spinal loading during industrial work have focused on estimating peak spinal compressive forces under static and sagittally symmetric conditions. The main objective of this study was to explore the potential of feasibly predicting three-dimensional (3D) spinal loading in industry from various combinations of trunk kinematics, kinetics, and subject-load characteristics. The study used spinal loading, predicted by a validated electromyography-assisted model, from 11 male participants who performed a series of symmetric and asymmetric lifts. Three classes of models were developed: (a) models using workplace, subject, and trunk motion parameters as independent variables (kinematic models); (b) models using workplace, subject, and measured moments variables (kinetic models); and (c) models incorporating workplace, subject, trunk motion, and measured moments variables (combined models). The results showed that peak 3D spinal loading during symmetric and asymmetric lifting were predicted equally well using all three types of regression models. Continuous 3D loading was predicted best using the combined models. When the use of such models is infeasible, the kinematic models can provide adequate predictions. Finally, lateral shear forces (peak and continuous) were consistently underestimated using all three types of models. The study demonstrated the feasibility of predicting 3D loads on the spine under specific symmetric and asymmetric lifting tasks without the need for collecting EMG information. However, further validation and development of the models should be conducted to assess and extend their applicability to lifting conditions other than those presented in this study. Actual or potential applications of this research include exposure assessment in epidemiological studies, ergonomic intervention, and laboratory task assessment. [ABSTRACT FROM AUTHOR]

Many commercial vehicles have steps and grab-rails to assist the driver in safely entering/exiting the vehicle. However, many drivers do not use these aids. The purpose of this study was to compare impact forces experienced during various exit methods from commercial equipment. The study investigated impact forces of ten male subjects while exiting two tractors, a step-van, a box-trailer, and a cube-van. The results showed that exiting from cab-level or trailer-level resulted in impact forces as high as 12 times the subject's body weight; whereas, fully utilizing the steps and grab-rails resulted in impact forces less than two times body weight. An approach that emphasizes optimal design of entry/exit aids coupled with driver training and education is expected to minimize exit-related injuries. [ABSTRACT FROM AUTHOR]

A relatively neglected topic in manual materials handling (MMH) research is the impact of the accuracy of task parameter measurements on the application of various assessment methods. A laboratory experiment was conducted to investigate the accuracy of NIOSH equation parameter measurements made by eight subjects following a 4-h training session. Five individual tasks were measured; two were single tasks and three were part of a multiple-component simulated palletizing operation. Significant differences between reference parameter measurements and average measurements made by subjects were found. The sensitivity analysis showed that frequency and horizontal location are the most important parameters. These parameters also tended to have the highest measurement errors. Recommendations for increasing the effectiveness of training programs for NIOSH equation users based on the results of the study are presented. [ABSTRACT FROM AUTHOR]

This study measured the effect of a feedback training program on lumbar compression during simulated occupational lifting. Two distinct types of feedback were compared: real-time electromyographic feedback, vs. an acceleration index delivered verbally post-lift. Kinematic data were collected from 28 subjects during symmetrical sagittal plane lifts. Following a baseline session, two feedback training sessions were provided, with a 1-week interval between each session. A control group followed the same protocols, but without receiving feedback training. A post-training session, using protocols identical to the baseline session, was used to assess pre-to-post changes in the dependent variable: peak lumbosacral compression computed using a dynamic linked-segment model. All three groups showed reductions in peak compression from pre-to-post: on average the control group improved by 11.2%, the EMG group by 16.7%, and the acceleration group by 25.3%. The results revealed an interaction between the improvement and the group (p=0.023), and a difference between the improvement in the control group and that in the verbal acceleration feedback group (p<0.01). These reductions in lumbosacral compression persisted after a 7-day interval without feedback training, suggesting that this approach could provide sustained risk-reduction during manual materials handling.

In epidemiologic studies on musculoskeletal disorders, some risk factors, especially physical load, cannot be determined independently from the worker. Posture, movement and external load are the result both of physical work requirements forced on the worker and of the worker's capacity to adopt particular techniques. Risk factors are also adjusted in relation to the worker's health. This paper presents a dynamic model that links exposure to risk factors for back pain and disability. Its aim is to help identify core elements in exposure assessment strategies for epidemiologic studies on back disorders. In this dynamic model, risk factors are determined relative to health status in order to distinguish between etiological and prognostic factors. Measurement techniques for various risk factors are classified into self-reports, observations, and direct instrumentation. Features of commonly used techniques are discussed with respect to feasibility, accuracy, and precision. In addition, consideration is given to the optimum allocation of measurements taking into account the effects of random and systematic variation in exposure due to tasks, workplaces, and workers.

Diurnal variation in trunk flexibility, defined as changes in range of motion, has been previously observed and quantified. Prior studies have shown that decreased disk height occurs as the day progresses and allows the spinal ligaments to slacken, resulting in an increase in the range of motion (flexibility) and a possible reduction in the risk of injury. This risk may be accentuated under dynamic motions of the spine. This study was conducted to observe the change in dynamic trunk mobility as a function of time of the day. Trunk motions of 21 men were observed at three specific times of the day using a triaxial electrogoniometer. No variation in trunk range of motion in any of the cardinal planes was observed. However, velocity and acceleration in the sagittal plane showed significant variations, suggesting the reexamination of the "slack ligaments" hypothesis. This study asserts that identifying flexibility by only its static component, range of motion, gives only partial information about the diurnal variation experienced by the spine. Industrial injuries occurring in the early morning hours may be a result of insufficient trunk mobility. This study indicates that risk associated with diurnal variation is far more complex than originally thought.

A continuing challenge for ergonomists has been to determine quantitatively the types of trunk motion and how much trunk motion contributes to the risk of occupationally-related low back disorder (LBD). It has been difficult to include this motion information in workplace assessments since the speed at which trunk motion becomes dangerous has not been determined. An in vivo study was performed to assess the contribution of three-dimensional dynamic trunk motions to the risk of LBD during occupational lifting in industry. Over 400 industrial lifting jobs were studied in 48 varied industries. The medical records in these industries were examined so that specific jobs historically categorized as either low, medium, or high risk for occupationally-related LBD could be identified. A tri-axial electrogoniometer was worn by workers and documented the three-dimensional angular position, velocity, and acceleration characteristics of the lumbar spine while workers worked at these low, medium, or high risk jobs. Workplace and individual characteristics were also documented for each of the repetitive lifting tasks. A multiple logistic regression model indicated that a combination of five trunk motion and workplace factors predicted well both medium risk and high risk occupational-related LBD. These factors included lifting frequency, load moment, trunk lateral velocity, trunk twisting velocity, and trunk sagittal angle. Increases in the magnitude of these factors significantly increased the risk of LBD. The analyses have enabled us to determine the LBD risk associated with combined changes in the magnitudes of the five factors. The results indicate that by suitably varying these five factors observed during the lift collectively, the odds of high risk group membership may decrease by over ten times. These results were related to the biomechanical, ergonomic, and epidemiologic literature. The five trunk motion and workplace factors could be used as quantitative, objective measures to redesign the workplace so that the risk of occupationally-related LBD is minimized.