Search

Abstract The evolution of morphinan alkaloid biosynthesis in plants of the genus Papaver includes permutation of several processes including gene duplication, fusion, neofunctionalization, and deletion resulting in the present chemotaxonomy. A critical gene fusion event resulting in the key bifunctional enzyme reticuline epimerase (REPI), which catalyzes the stereochemical inversion of (S)-reticuline, was suggested to precede neofunctionalization of downstream enzymes leading to morphine biosynthesis in opium poppy (Papaver somniferum). The ancestrally related aldo-keto reductases 1,2-dehydroreticuline reductase (DRR), which occurs in some species as a component of REPI, and codeinone reductase (COR) catalyze the second and penultimate steps, respectively, in the pathway converting (S)-reticuline to morphine. Orthologs for each enzyme isolated from the transcriptomes of 12 Papaver species were shown to catalyze their respective reactions in species that capture states of the metabolic pathway prior to key evolutionary events, including the gene fusion event leading to REPI, thus suggesting a patchwork model for pathway evolution. Analysis of the structure and substrate preferences of DRR orthologs in comparison with COR orthologs revealed structure-function relationships underpinning the functional latency of DRR and COR orthologs in the genus Papaver, thus providing insights into the molecular events leading to the evolution of the pathway.

Abstract Background We aimed to study the impact of biological therapies in Saudi Arabia on patients with severe asthma (SA) combined with chronic rhinosinusitis (CRS) in terms of clinical outcomes. Methods This is a retrospective observational cohort research that was undertaken at the severe asthma clinics of the Armed Forces Hospital of the Southern Region (AFHSR) and King Khalid University Hospital, Abha, from March to September 2022 to delineate the effects of 3 biological therapies (dupilumab, benralizumab, and omalizumab) in adults with SA and concomitant CRS. Clinical outcomes assessed included asthma exacerbation frequency, hospitalization rates, use of oral corticosteroids (OCs), and the asthma control test (ACT) scores before and 1 year after biological therapies. Results Eighty patients were enrolled, with a mean age of 46.68. There were 45 (56%) females and 35 (44%) males. There was a notifiable decrease in the frequency of exacerbations and hospitalization and in the number of patients who received OCs after 6 and 12 months of biological therapies compared to pre-biological therapies, respectively (p

This article adopts a process evaluation approach to identify the challenges and opportunities in scaled implementation of early childhood education (ECE) in Pakistan's most populous province, Punjab. It reports findings from mixed methods, including surveys administered to head teachers, ECE teachers, and parents across 386 schools, and focus groups and in-depth interviews with 25 participants across the different tiers of government. Using the RISE framework as an analytical lens, the article specifically explores issues of delegation, finance, information, and motivation across two accountability relationships - Compact and Management - to assess the extent to which the province's early learning system is coherent around the goal of scaling quality early learning. Research findings reveal multiple incoherences within each relationship of accountability as well as incoherences within similar elements across the two accountability relationships. The resulting insights indicate the need to strengthen the province's early learning system and improve the flow of accountability through effective delegation, including improved coordination, communication, and feedback mechanisms; effective finance, including resources for capacitating actors across all tiers of government; effective information, especially collection and utilization of data on process quality; and effective motivation to address weaker links in teacher allocation, incentives, professional development, and academic support.

PURPOSE: To assess the relationship between qualitative diabetic retinopathy (DR) scales with the precise numbers and surface area of DR lesions within the Early Treatment Diabetic Retinopathy Study (ETDRS) standard seven field (S7F) region on ultrawide-field (UWF) color fundus images. METHODS: In this study, we collected UWF images from adult patients with diabetes. Poor-quality images and eyes with any pathology precluding assessment of DR severity were excluded. The DR lesions were manually segmented. DR severity was graded according to the International Clinical Diabetic Retinopathy (ICDR) and AA protocol by two masked graders within the ETDRS S7F. These lesions numbers and surface area were computed and correlated against the DR scores using the Kruskal-Wallis H test. Cohens Kappa was performed to determine the agreement between two graders. RESULTS: One thousand five hundred and twenty eyes of 869 patients (294 females, 756 right eyes) with a mean age of 58.7 years were included. 47.4% were graded as no DR, 2.2% as mild non-proliferative DR (NPDR), 24.0% as moderate NPDR, 6.3% as severe NPDR, and 20.1% as proliferative DR (PDR). The area and number of DR lesions generally increased as the ICDR level increased up to severe NPDR, but decreased from severe NPDR to PDR. There was perfect intergrader agreement on the DR severity. CONCLUSION: A quantitative approach reveals that DR lesions number and area generally correlate with ICDR-based categorical DR severity levels with an increasing trend in the number and area of DR lesions from mild to severe NPDR and a decrease from severe NPDR to PDR.

Abstract This research is focused on the development of an eco-friendly low-cost concrete using fly ash (FA) and marble powder waste (MPW) as partial replacements for cement and fine aggregate respectively. The substantial use of cement in concrete makes it expensive and contributes to global warming due to high carbon emissions. Thus, using such waste materials can help reduce the overall carbon footprint. For this purpose, various mix designs of concrete were developed by varying the percentages of FA and MPW. The concrete's fresh and hardened properties were experimentally determined for those mixes. The test results revealed that MPW as a sand substitute increases strength up to 40% and gradually decreases beyond that, but a 60% replacement still has more strength than the control specimen. Similarly, using FA as a cement replacement was found to reduce the strength, but the reduction was not very significant up to 20%. A mixed blend of FA and MPW showed superior results and maximum strength was obtained at F10M40. The optimal mix, with 10% FA and 40% MPW (F10M40), achieved a compressive strength of 4493.46 psi, a 16.21% improvement compared to the control mix proportion. Furthermore, the microstructure of the cementitious material was improved due to the pozzolanic reaction that led to a denser microstructure, as supported by the permeability test and SEM analysis.

Abstract Nematodes serve as key indicators of soil health in ecological studies. Therefore, the current study examined the community structure and metabolic footprint of soil nematodes in alfalfa fields across varying levels of N supply in the semi-arid Loess Plateau. The findings offer theoretical guidance for the sustainable management of artificial alfalfa grasslands in this region. The research was based on alfalfa fields with different N application rates (0, 50, 100, 150 kg/ha2) as the research object, The shallow plate method was used to separate and extract soil nematodes, identify soil nematode groups, calculate ecological function index and metabolic footprint, and identify indicator species. A total of 6346 nematodes were isolated in this study, belonging to 27 genera and 19 families. Notably, the plant parasitic nematode Helicotylenchus was predominant. As N addition increased, the plant parasitic index (PPI) increased significantly. A N50 application significantly enhanced the soil nematode diversity index ( $$\text{H}$$ H ) and the free-living index (MI). The findings showcased a noticeable decrease in disturbance within the N50 soil nematode community. This resulted in a mature and stable community structure primarily attributed to the heightened abundance of omnivorous/predatory nematodes. Across various N levels, soil nematode communities underwent significant alterations in the soil food web structure through shifts in their metabolic footprint. Future strategies should focus on refining N management practices and integrating sustainable approaches like crop rotation and pest management. These efforts will contribute to guidelines ensuring artificial alfalfa grasslands lasting health and productivity.

Abstract Background This study’s purposes were to evaluate the impact of biological therapies on outcomes in patients with severe asthma (SA) and chronic rhinosinusitis (CRS) and to compare these effects among those with NP (CRSwNP) versus those without NP (CRSsNP) in the “real-world” setting in Saudi Arabian patients. Methods From March to September 2022, a retrospective observational cohort study was undertaken at the severe asthma clinics of the Armed Forces Hospital—Southern Region (AFHSR) and King Khalid University Hospital, Abha, Saudi Arabia, to delineate the effects of dupilumab therapy. Outcomes were assessed, including clinical outcomes, FEV1, and laboratory findings before and one year after dupilumab. Post-therapy effects were compared between CRSwNP and CRSsNP. Results Fifty subjects were enrolled, with a mean age of 46.56. There were 27 (54%) females and 23(46%) males. Significant improvements in clinical parameters (frequency of asthma exacerbations and hospitalizations, the use of OCs, anosmia, SNOTT-22, and the ACT), FEV1, and laboratory ones (serum IgE and eosinophilic count) were observed 6 and 12 months after using dupilumab (p

Introduction: In recent years, exposure to nanomaterials has been known as a challenge among occupational health experts. In this line, personal protective equipment has been considered as a solution to reduce the worker’s exposure. Since respiratory and skin tracts represent the most common workplace exposure routes, knowledge of the efficiency of respiratory and skin protection equipment is particularly important. So, the aim of this study is the assessment of the efficiency of respiratory and skin protection equipment toward controlling nanoparticles in the workplace with a scoping review approach. Material and Methods: This study was conducted in 2022 with a scoping review approach. Arksey and O’Malley’s five-step framework was chosen as the research method. The search strategy was followed in the databases necessary to access the research data, including PubMed, Google Scholar, Science Direct, Web of Science, and Scopus. Also, EndNote X9® and Microsoft Excel software were used to collect and analyze studies, respectively. Results: In the first step, 1014 articles were identified. Finally, 38 articles were included in the study to examine quantitative and qualitative information about the efficiency of respiratory and skin protective equipment. Twenty-five articles were related to breathing masks, and six studies were about protective gloves, and seven other articles were devoted to protective clothing. According to the studies of breathing masks, the mean total penetration of nanoparticles was estimated at 2.27%. Also, based on the studies of protective clothing, the maximum penetration of nanoparticles was 30nm for protective clothing made of polypropylene and polyethylene with different layers. Conclusion: Although the efficiency of existing personal protective equipment showed a good result for controlling nanoparticles, the size of nanoparticles is one of the essential parameters in determining the efficiency of the equipment, which should be considered the workplaces. So, it is recommended that more studies be considered to improve their efficiency, and standard tests should be developed to evaluate them.

A crane system with distributed mass payload (DMP) is highly nonlinear and it is a multi-mode system with different oscillation frequencies. The crane dynamics are also largely affected when operating under different cable and payload lengths. This paper investigates robust multi-mode input shapers for effective oscillation control of an overhead crane carrying DMP. Three robust shapers which are Zero Vibration Derivative (ZVD), Extra-Insensitive (EI) and Equal Shaping-Time and Magnitude (ETM) are considered and modified to be multi-mode shapers. These are obtained by convolving two input shapers that are designed separately based on the hook and payload oscillation frequencies. To assess the effectiveness of the shapers, Matlab simulations are performed by using the nonlinear dynamic model of the crane and applied under three distinct cases involving different cable and payload lengths. Simulation results show that the multi-mode EI shaper (MM-EI) has the highest performance in reduction of the hook and payload oscillations, with the lowest maximum transient sway and mean average error. In addition, with 40% errors in the oscillation frequencies, the MM-EI provides the highest robustness by achieving 50% reductions in the payload oscillations as compared to the case with an unshaped input.

Kelapa sawit ialah satu di antara komoditas perkebunan yang berperan penting pada perekonomian Indonesia maupun dunia. Produktivitas kelapa sawit sangat dipengaruhi oleh kualitas Tandan Buah Segar (TBS). Tantangan yang dihadapi oleh perusahaan industri kelapa sawit adalah pemilihan TBS yang masih menggunakan metode manual yang rentan dengan kesalahan dan inkonsistensi manusia. Tantangan tersebut juga dirasakan oleh PT Arya Rama Prakarsa. Tujuan penelitian ini adalah untuk membantu mempermudah manajemean dalam pengambilan keputusan serta memberikan gambaran mengenai kualitas TBS yang akan diproduksi, Preference Ranking Organization Method for Enrichment Evaluation (PROMETHEE) adalah metode yang diimplementasikan pada penelitian ini. Berdasarkan Penilitian yang telah dilakukan didapatkan hasil bahwa Dari persamaan hasil akumulasi perhitungan menggunakan Microsoft Excel dan Sistem menunjukkan hasil yang sama, yaitu alterntif TBS CV. Restu Bangun Persada Menempati peringkat pertama dengan nilai tertinggi 0,36 yang berarti TBS tersebut layak untuk diproduksi. Dapat disimpulkan bahwa penelitian yang telah dilakukan, terbukti efektif untuk pemilihan tandan buah segar (TBS) kelapa sawit yang layak produksi di PT Arya Rama Prakarsa. Kata Kunci: Pemilihan TBS, PROMETHEE, Sistem Pendukung Keputusan

With the intensification of global change, forests are subjected to varying degrees of drought or high-temperature stress, which has an indelible impact on the growth of trees. However, knowledge on the response of sap flow to environmental changes in different types of forests is still rare, especially in China's subtropical forest ecosystem. Consequently, studying how different tree species regulate their sap flow in response to shifting environmental conditions is essential for understanding forest transpiration, water use efficiency, and drought stress resilience. Therefore, this study aimed to investigate the sap flow dynamics of seven tree species in five forest plots, i.e., pine forest (PF), two types of mixed conifer-broadleaf forests (MF1+MF2), monsoon evergreen broadleaved forest (MEBF), and montane monsoonal evergreen broad-leaf forest (MOBF) at Dinghushan National Reserve in Southern China, using the heat dissipation probe technique and synchronous environmental factor recordings. Results demonstrated a significant influence of photosynthetic active radiation (PAR) on sap flow across all tree species, with mean PAR values ranging from over 1200 to 425 μmol m−2 s−1, establishing it as the principal driving factor. This observation underscores the heightened responsiveness or sensitivity of tree species to variations in PAR as the forest undergoes development and maturation. The correlation between vapor pressure deficit (VPD) and tree sap flow decreased as succession progressed. Moreover, the influence of soil water content (SWC) on sap flow stability against environmental changes increased. Similar patterns were also found between the two MF, with MF-2 displaying ecological characteristics and environmental conditions more closely aligned with those of the late-successional MEBF. The study reveals the intricate relationship between environmental factors and sap flow regulation in tree species within a subtropical forest ecosystem. Addressing a comparative gap in sap flow correlation among dominant tree species at Dinghushan, it establishes a hydro-physiological foundation for understanding tree species substitution during forest succession. The results provide key insights for forest management and climate-related research. Future studies should delve into the long-term implications of observed sap flow dynamics, exploring their impact on tree species adaptability amid ongoing environmental changes.

Abstract With the development of deep learning, a higher level of perception of the environment such as the semantic level can be achieved in the simultaneous localization and mapping (SLAM) domain. However, previous works did not achieve a natural-language level of perception. Therefore, LP-SLAM (Language-Perceptive RGB-D SLAM) is proposed that leverages large language models (LLMs). The texts in the scene can be detected by scene text recognition (STR) and mapped as landmarks with a task-driven selection. A text error correction chain (TECC) is designed with a similarity classification method, a two-stage memory strategy, and a text clustering method. The proposed architecture is designed to deal with the mis-detection and mis-recognition cases of STR and to provide accurate text information to the framework. The proposed framework takes input images and generates a 3D map with sparse point cloud and task-related texts. Finally, a natural user interface (NUI) is designed based on the constructed map and LLM, which gives position instructions based on users’ natural queries. The experimental results validated the proposed TECC design and the overall framework. We publish the virtual dataset with ground truth, as well as the source code for further research. https://github.com/GroupOfLPSLAM/LP_SLAM .

Abstract Background Educating health professionals on patient safety can potentially reduce healthcare-associated harm. Patient safety courses have been incorporated into medical and nursing curricula in many high-income countries and their impact has been demonstrated in the literature through objective assessments. This study aimed to explore student perceptions about a patient safety course to assess its influence on aspiring health professionals at a personal level as well as to explore differences in areas of focus between medical and nursing students. Methods A dedicated patient safety course was introduced for year III medical and year II and IV nursing students at the Aga Khan University (2021–2022). As part of a post-course assessment, 577 participating students (184 medical and 393 nursing) wrote reflections on the course, detailing its influence on them. These free-text responses were thematically analyzed using NVivo. Results The findings revealed five major themes: acquired skills (clinical, interpersonal), understanding of medical errors (increased awareness, prevention and reduction, responding to errors), personal experiences with patient safety issues, impact of course (changed perceptions, professional integrity, need for similar sessions, importance of the topic) and course feedback (format, preparation for clinical years, suggestions). Students reported a lack of baseline awareness regarding the frequency and consequences of medical errors. After the course, medical students reported a perceptional shift in favor of systems thinking regarding error causality, and nursing students focused on human factors and error prevention. The interactive course format involving scenario-based learning was deemed beneficial in terms of increasing awareness, imparting relevant clinical and interpersonal skills, and changing perspectives on patient safety. Conclusions Student perspectives illustrate the benefits of an early introduction of dedicated courses in imparting patient safety education to aspiring health professionals. Students reported a lack of baseline awareness of essential patient safety concepts, highlighting gaps in the existing curricula. This study can help provide an impetus for incorporating patient safety as a core component in medical and nursing curricula nationally and across the region. Additionally, patient safety courses can be tailored to emphasize areas identified as gaps among each professional group, and interprofessional education can be employed for shared learning. The authors further recommend conducting longitudinal studies to assess the long-term impact of such courses.

Abstract Heavy metals (HMs) contamination, owing to their potential links to various chronic diseases, poses a global threat to agriculture, environment, and human health. Nickel (Ni) is an essential element however, at higher concentration, it is highly phytotoxic, and affects major plant functions. Beneficial roles of plant growth regulators (PGRs) and organic amendments in mitigating the adverse impacts of HM on plant growth has gained the attention of scientific community worldwide. Here, we performed a greenhouse study to investigate the effect of indole-3-acetic acid (IAA @ 10− 5 M) and compost (1% w/w) individually and in combination in sustaining cauliflower growth and yield under Ni stress. In our results, combined application proved significantly better than individual applications in alleviating the adverse effects of Ni on cauliflower as it increased various plant attributes such as plant height (49%), root length (76%), curd height and diameter (68 and 134%), leaf area (75%), transpiration rate (36%), stomatal conductance (104%), water use efficiency (143%), flavonoid and phenolic contents (212 and 133%), soluble sugars and protein contents (202 and 199%), SPAD value (78%), chlorophyll ‘a and b’ (219 and 208%), carotenoid (335%), and NPK uptake (191, 79 and 92%) as compared to the control. Co-application of IAA and compost reduced Ni-induced electrolyte leakage (64%) and improved the antioxidant activities, including APX (55%), CAT (30%), SOD (43%), POD (55%), while reducing MDA and H2O2 contents (77 and 52%) compared to the control. The combined application also reduced Ni uptake in roots, shoots, and curd by 51, 78 and 72% respectively along with an increased relative production index (78%) as compared to the control. Hence, synergistic application of IAA and compost can mitigate Ni induced adverse impacts on cauliflower growth by immobilizing it in the soil.

OBJECTIVE: To determine the significance of etiological basis of thyrotoxicosis in guiding optimal treatment selection and its correlation with age and the presence of goiter. METHODS: This cross-sectional study was conducted at MTI-Hayatabad Medical Complex in Peshawar, Pakistan, from December 2022 to May 2023. Utilizing nonprobability-consecutive sampling, we enrolled 119 participants, focusing on individuals presenting with thyrotoxicosis confirmed by thyroid function tests (TFTs). Those already undergoing treatment for hyperthyroidism were also included. Essential diagnostic assessments, such as radioiodine scans, erythrocyte sedimentation rate (ESR), and thyroid receptor stimulating antibody tests, were performed as needed. Patients with chronic renal disease, chronic liver disease, and pregnant and lactating women were excluded. RESULTS: The mean age of participants was 40.39±13.65 years, with 84.03% having TSH

This study aims to develop a finite element model for accurately predicting the behavior of RC beams retrofitted and strengthened with CFRP strips. Two types of CFRP layout are modelled, CFRP wraps at the bottom without any anchorage, and CFRP strips with CFRP wrap as anchorage. The developed numerical models are validated by experimentally testing RC beam models for four-point bending. The concrete damage plasticity model is used for introducing non-linearity in concrete. The non-linear tensile and compressive parameters are taken from the ABAQUS manual specifications. The interface between the CFRP and RC beam surface was replicated using a perfect bond between the two. The results show that the developed numerical model accurately predicts the strength of retrofitted and strengthened beams. Though numerical models show higher initial stiffnesses and cracking load, especially in the case of a retrofitted specimen, where the initial stiffness is as high as 75%.

Abstract Lead (Pb) is toxic to the development and growth of rice plants. Nanoparticles (NPs) have been considered one of the efficient remediation techniques to mitigate Pb stress in plants. Therefore, a study was carried out to examine the underlying mechanism of iron (Fe) and silicon (Si) nanoparticle-induced Pb toxicity alleviation in rice seedlings. Si–NPs (2.5 mM) and Fe-NPs (25 mg L−1) were applied alone and in combination to rice plants grown without (control; no Pb stress) and with (100 µM) Pb concentration. Our results revealed that Pb toxicity severely affected all rice growth-related traits, such as inhibited root fresh weight (42%), shoot length (24%), and chlorophyll b contents (26%). Moreover, a substantial amount of Pb was translocated to the above-ground parts of plants, which caused a disturbance in the antioxidative enzyme activities. However, the synergetic use of Fe- and Si–NPs reduced the Pb contents in the upper part of plants by 27%. It reduced the lethal impact of Pb on roots and shoots growth parameters by increasing shoot length (40%), shoot fresh weight (48%), and roots fresh weight (31%). Both Si and Fe–NPs synergistic application significantly elevated superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and glutathione (GSH) concentrations by 114%, 186%, 135%, and 151%, respectively, compared to plants subjected to Pb stress alone. The toxicity of Pb resulted in several cellular abnormalities and altered the expression levels of metal transporters and antioxidant genes. We conclude that the synergistic application of Si and Fe-NPs can be deemed favorable, environmentally promising, and cost-effective for reducing Pb deadliness in rice crops and reclaiming Pb-polluted soils.

We combined carbon dioxide (CO2)-responsive cytosine-containing rhodamine 6G (Cy-R6G) as a hydrophobic anticancer agent with hydrogen-bonded cytosine-functionalized polyethylene glycol (Cy-PEG) as a hydrophilic supramolecular carrier to construct a CO2-responsive drug delivery system, with the aim of enhancing the responsiveness of the system to the tumor microenvironment and thus the overall effectiveness of anticancer therapy. Due to self-complementary hydrogen bonding interactions between cytosine units, Cy-R6G and Cy-PEG co-assemble in water to form spherical-like nanogels, with Cy-R6G effectively encapsulated within the nanogels. The nanogels exhibit several distinctive physical features, such as widely tunable nanogel size and drug loading capacity for Cy-R6G, intriguing fluorescence properties, high co-assembled structural stability in normal aqueous environments, enhanced anti-hemolytic characteristics, sensitive dual CO2/pH-responsive behavior, and precise and easily controllable CO2-induced release of Cy-R6G. Cytotoxicity assays clearly indicated that, due to the presence of cytosine receptors on the surface of cancer cells, Cy-R6G-loaded nanogels exert selective cytotoxicity against cancer cells in pristine culture medium, but do not affect the viability of normal cells. Surprisingly, in CO2-rich culture medium, Cy-R6G-loaded nanogels exhibit a further significant enhancement in cytotoxicity against cancer cells, and remain non-cytotoxic to normal cells. More importantly, a series of in vitro experiments demonstrated that compared to pristine culture medium, CO2-rich culture medium promotes more rapid selective internalization of Cy-R6G-loaded nanogels into cancer cells through cytosine-mediated macropinocytosis and thus accelerates the induction of apoptosis. Therefore, this newly developed system provides novel avenues for the development of highly effective CO2-responsive drug delivery systems with potent anticancer capabilities.

Purpose: Conjunctival melanoma is a rare neoplasm with high rates of recurrence and metastasis. Traditional management includes surgical excision and cryotherapy, followed by adjuvant therapy as needed. Immune checkpoint inhibitors, including nivolumab, are a targeted treatment option with improved survival rates. However, various immune-related adverse effects have been reported with these drugs. While systemic granulomatous inflammation is a documented systemic side effect, it has rarely been reported in the conjunctiva and ocular adnexa. Observation: A patient with a history of recurrent metastatic conjunctival melanoma presented with both a left sub-conjunctival and upper eyelid lesion after the commencement of treatment with nivolumab. The lesions were excised with a clinical suspicion for metastasis and consisted of noncaseating granulomatous inflammation with no evidence of malignancy on histopathologic examination. Infectious and primary autoimmune etiologies were ruled out. Conclusion and importance: This is a biopsy-proven case of periocular immune checkpoint inhibitor-associated granulomatous inflammation.

Iron-nanoparticles (Fe-NPs) are increasingly been utilized in environmental applications due to their efficacy and strong catalytic activities. The novelty of nanoparticle science had attracted many researchers and especially for their green synthesis, which can effectively reuse biological resources during the polymerization reactions. Thus, the synthesis of Fe-NPs utilizing plant extracts could be considered as the eco-friendly, simple, rapid, energy-efficient, sustainable, and cost-effective. The green synthesis route can be recognized as a practical, valuable, and economically effective alternative for large-scale production. During the production process, some biomolecules present in the extracts undergo metal salts reduction, which can serve as both a capping and reducing mechanism, enhancing the reactivity and stability of green-synthesized Fe-NPs. The diversity of species provided a wide range of potential sources for green synthesis of Fe-NPs. With improved understanding of the specific biomolecules involved in the bioreduction and stabilization processes, it will become easier to identify and utilize new, potential plant materials for Fe-NPs synthesis. Newly synthesized Fe-NPs require different characterization techniques such as transmission electron microscope, ultraviolet-visible spectrophotometry, and X-ray absorption fine structure, etc, for the determination of size, composition, and structure. This review described and assessed the recent advancements in understanding green-synthesized Fe-NPs derived from plant-based material. Detailed information on various plant materials suitable of yielding valuable biomolecules with potential diverse applications in environmental safety. Additionally, this review examined the characterization techniques employed to analyze Fe-NPs, their stability, accumulation, mobility, and fate in the environment. Holistically, the review assessed the applications of Fe-NPs in remediating wastewaters, organic residues, and inorganic contaminants. The toxicity of Fe-NPs was also addressed; emphasizing the need to refine the synthesis of green Fe-NPs to ensure safety and environmental friendliness. Moving forward, the future challenges and opportunities associated with the green synthesis of Fe-NPs would motivate novel research about nanoparticles in new directions.

Multiwall carbon nanotubes (MWCNTs) were modified through deferoxamine mesylate and used as an adsorbent for separation and preconcentration of the trace amount of iron ions in food samples followed by a simple, fast and sensitive spectrophotometric method was developed for the termination. The proposed separation and preconcentration method was performed in short time by using a small amount of chemical solvent. Also, the used adsorbent was environmentally friendly and highly compatible with the human body.In this study iron was complex with deferoxamine. After extraction, the complex was eluted by HCl 2 % (v/v) and analyzed by a fast, straightforward, and inexpensive UV–Visible spectrometer method. The determination was performed with high selectivity, sensitivity and good accuracy. The calibration curve was linear in the range of 0.12 µg.L−1 to 500 µg.L−1. The detection limit was obtained 0.08 µg.L−1. The suggested method was used for measuring the trace amount of iron ions in the five food samples: spinach, parsley, cooked pinto beans, and cooked sheep's liver. Based on the obtained results, the proposed method has shown proper sensitivity, accuracy, and repeatability.

The development of new carbon-based materials using natural biowaste for biomedical applications has remained a significant challenge in the past decades. In this study, we successfully synthesized and characterized composite materials made from peanut shell-derived carbon (PNS–C) decorated on ZnO that formed star-shaped particles via a simple hydrothermal technique. The as-prepared composites possess several advantages, including unique optical properties and high photostability. We evaluate the antibacterial performance against Escherichia coli, a gram-negative bacterium and Staphylococcus aureus, a gram-positive bacterium, under irradiated and non-irradiated conditions. Interestingly, the photo-antibacterial activities of ZnO/PNS-C composites showed great inhibition of bacterial growth as compared to pure ZnO. Moreover, significant disruptions in cellular activities occur when the composites make direct contact with the bacterial cell wall. The electrons and holes produced by excitation in composites provide a pronounced deactivating effect on bacterial activity. In addition, ZnO/PNS-C composites are highly biocompatible with normal cells. Thus, these newly developed composites made from a natural biowaste system with an affordable price, abundance, and non-toxicity could provide a potentially environmentally friendly and fruitful route for antibacterial therapy in future applications.