7 results on '"Farrugia TJ"'
Search Results
2. Fluorescent Molecular Logic Gates and Pourbaix Sensors in Polyacrylamide Hydrogels.
- Author
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Scerri GJ, Caruana M, Agius N, Agius G, Farrugia TJ, Spiteri JC, Johnson AD, and Magri DC
- Subjects
- Acrylic Resins, Amines, Ammonium Sulfate, Anthracenes, Hydrogels chemistry, Oxidants, Coloring Agents, Methanol
- Abstract
Polyacrylamide hydrogels formed by free radical polymerisation were formed by entrapping anthracene and 4-amino-1,8-naphthalimide fluorescent logic gates based on photoinduced electron transfer (PET) and/or internal charge transfer (ICT). The non-covalent immobilisation of the molecules in the hydrogels resulted in semi-solid YES, NOT, and AND logic gates. Two molecular AND gates, examples of Pourbaix sensors, were tested in acidic aqueous methanol with ammonium persulfate, a strong oxidant, and displayed greater fluorescence quantum yields than previously reported. The logic hydrogels were exposed to aqueous solutions with chemical inputs, and the fluorescence output response was viewed under 365 nm UV light. All of the molecular logic gates diffuse out of the hydrogels to some extent when placed in solution, particularly those with secondary basic amines. The study exemplifies an effort of taking molecular logic gates from homogeneous solutions into the realm of solid-solution environments. We demonstrate the use of Pourbaix sensors as pE-pH indicators for monitoring oxidative and acidic conditions, notably for excess ammonium persulfate, a reagent used in the polymerisation of SDS-polyacrylamide gels.
- Published
- 2022
- Full Text
- View/download PDF
3. Diving into the vertical dimension of elasmobranch movement ecology.
- Author
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Andrzejaczek S, Lucas TCD, Goodman MC, Hussey NE, Armstrong AJ, Carlisle A, Coffey DM, Gleiss AC, Huveneers C, Jacoby DMP, Meekan MG, Mourier J, Peel LR, Abrantes K, Afonso AS, Ajemian MJ, Anderson BN, Anderson SD, Araujo G, Armstrong AO, Bach P, Barnett A, Bennett MB, Bezerra NA, Bonfil R, Boustany AM, Bowlby HD, Branco I, Braun CD, Brooks EJ, Brown J, Burke PJ, Butcher P, Castleton M, Chapple TK, Chateau O, Clarke M, Coelho R, Cortes E, Couturier LIE, Cowley PD, Croll DA, Cuevas JM, Curtis TH, Dagorn L, Dale JJ, Daly R, Dewar H, Doherty PD, Domingo A, Dove ADM, Drew M, Dudgeon CL, Duffy CAJ, Elliott RG, Ellis JR, Erdmann MV, Farrugia TJ, Ferreira LC, Ferretti F, Filmalter JD, Finucci B, Fischer C, Fitzpatrick R, Forget F, Forsberg K, Francis MP, Franks BR, Gallagher AJ, Galvan-Magana F, García ML, Gaston TF, Gillanders BM, Gollock MJ, Green JR, Green S, Griffiths CA, Hammerschlag N, Hasan A, Hawkes LA, Hazin F, Heard M, Hearn A, Hedges KJ, Henderson SM, Holdsworth J, Holland KN, Howey LA, Hueter RE, Humphries NE, Hutchinson M, Jaine FRA, Jorgensen SJ, Kanive PE, Labaja J, Lana FO, Lassauce H, Lipscombe RS, Llewellyn F, Macena BCL, Mambrasar R, McAllister JD, McCully Phillips SR, McGregor F, McMillan MN, McNaughton LM, Mendonça SA, Meyer CG, Meyers M, Mohan JA, Montgomery JC, Mucientes G, Musyl MK, Nasby-Lucas N, Natanson LJ, O'Sullivan JB, Oliveira P, Papastamtiou YP, Patterson TA, Pierce SJ, Queiroz N, Radford CA, Richardson AJ, Richardson AJ, Righton D, Rohner CA, Royer MA, Saunders RA, Schaber M, Schallert RJ, Scholl MC, Seitz AC, Semmens JM, Setyawan E, Shea BD, Shidqi RA, Shillinger GL, Shipley ON, Shivji MS, Sianipar AB, Silva JF, Sims DW, Skomal GB, Sousa LL, Southall EJ, Spaet JLY, Stehfest KM, Stevens G, Stewart JD, Sulikowski JA, Syakurachman I, Thorrold SR, Thums M, Tickler D, Tolloti MT, Townsend KA, Travassos P, Tyminski JP, Vaudo JJ, Veras D, Wantiez L, Weber SB, Wells RJD, Weng KC, Wetherbee BM, Williamson JE, Witt MJ, Wright S, Zilliacus K, Block BA, and Curnick DJ
- Abstract
Knowledge of the three-dimensional movement patterns of elasmobranchs is vital to understand their ecological roles and exposure to anthropogenic pressures. To date, comparative studies among species at global scales have mostly focused on horizontal movements. Our study addresses the knowledge gap of vertical movements by compiling the first global synthesis of vertical habitat use by elasmobranchs from data obtained by deployment of 989 biotelemetry tags on 38 elasmobranch species. Elasmobranchs displayed high intra- and interspecific variability in vertical movement patterns. Substantial vertical overlap was observed for many epipelagic elasmobranchs, indicating an increased likelihood to display spatial overlap, biologically interact, and share similar risk to anthropogenic threats that vary on a vertical gradient. We highlight the critical next steps toward incorporating vertical movement into global management and monitoring strategies for elasmobranchs, emphasizing the need to address geographic and taxonomic biases in deployments and to concurrently consider both horizontal and vertical movements.
- Published
- 2022
- Full Text
- View/download PDF
4. A biologging database of juvenile white sharks from the northeast Pacific.
- Author
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O'Sullivan J, Lowe CG, Sosa-Nishizaki O, Jorgensen SJ, Anderson JM, Farrugia TJ, García-Rodríguez E, Lyons K, McKinzie MK, Oñate-González EC, Weng K, White CF, Winkler C, and Van Houtan KS
- Subjects
- Animals, Databases, Factual, Telemetry, Ecosystem, Sharks
- Abstract
Species occurrence records are vital data streams in marine conservation with a wide range of important applications. From 2001-2020, the Monterey Bay Aquarium led an international research collaboration to understand the life cycle, ecology, and behavior of white sharks (Carcharodon carcharias) in the southern California Current. The collaboration was devoted to tagging juveniles with animal-borne sensors, also known as biologging. Here we report the full data records from 59 pop-up archival (PAT) and 20 smart position and temperature transmitting (SPOT) tags that variously recorded pressure, temperature, and light-level data, and computed depth and geolocations for 63 individuals. Whether transmitted or from recovered devices, raw data files from successful deployments (n = 70) were auto-ingested from the manufacturer into the United States (US) Animal Telemetry Network's (ATN) Data Assembly Center (DAC). There they have attributed a full suite of metadata, visualized within their public-facing data portal, compiled for permanent archive under the DataONE Research Workspace member node, and are accessible for download from the ATN data portal., (© 2022. The Author(s).)
- Published
- 2022
- Full Text
- View/download PDF
5. Description of an elasmobranch TCR coreceptor: CD8α from Rhinobatos productus.
- Author
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Hansen JD, Farrugia TJ, Woodson J, and Laing KJ
- Subjects
- Amino Acid Motifs, Amino Acid Sequence, Animals, CD8 Antigens chemistry, Cloning, Molecular, Fish Proteins chemistry, Molecular Sequence Data, Phylogeny, Sequence Alignment, CD8 Antigens genetics, CD8 Antigens immunology, Elasmobranchii immunology, Fish Proteins genetics, Fish Proteins immunology
- Abstract
Cell-mediated immunity plays an essential role for the control and eradication of intracellular pathogens. To learn more about the evolutionary origins of the first signal (Signal 1) for T-cell activation, we cloned CD8α from an elasmobranch, Rhinobatos productus. Similar to full-length CD8α cDNAs from other vertebrates, Rhpr-CD8α (1800bp) encodes a 219 amino acid open reading frame composed of a signal peptide, an extracellular IgSF V domain and a stalk/hinge region followed by a well-conserved transmembrane domain and cytoplasmic tail. Overall, the mature Rhpr-CD8α protein (201 aa) displays ∼ 30% amino acid identity with mammalian CD8α including absolute conservation of cysteine residues involved in the IgSf V domain fold and dimerization of CD8αα and CD8αβ. One prominent feature is the absence of the LCK association motif (CXC) that is needed for achieving signal 1 in tetrapods. Both elasmobranch and teleost CD8α protein sequences possess a similar but distinctly different motif (CXH) in the cytoplasmic tail. The overall genomic structure of CD8α has been conserved during the course of vertebrate evolution both for the number of exons and phase of splicing. Finally, quantitative RTPCR demonstrated that elasmobranch CD8α is expressed in lymphoid-rich tissues similar to CD8 in other vertebrates. The results from this study indicate the existence of CD8 prior to the emergence of the gnathostomes (>450 MYA) while providing evidence that the canonical LCK association motif in mammals is likely a derived characteristic of tetrapod CD8α, suggesting potential differences for T-cell education and activation in the various gnathostomes., (Published by Elsevier Ltd.)
- Published
- 2011
- Full Text
- View/download PDF
6. Human influences on rates of phenotypic change in wild animal populations.
- Author
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Hendry AP, Farrugia TJ, and Kinnison MT
- Subjects
- Analysis of Variance, Animals, Databases, Factual, Geography, Humans, Species Specificity, Adaptation, Biological genetics, Environment, Human Activities, Phenotype
- Abstract
Human activities can expose populations to dramatic environmental perturbations, which may then precipitate adaptive phenotypic change. We ask whether or not phenotypic changes associated with human-disturbed (anthropogenic) contexts are greater than those associated with more 'natural' contexts. Our meta-analysis is based on more than 3000 rates of phenotypic change in 68 'systems', each representing a given species in a particular geographical area. We find that rates of phenotypic change are greater in anthropogenic contexts than in natural contexts. This difference may be influenced by phenotypic plasticity - because it was evident for studies of wild-caught individuals (which integrate both genetic and plastic effects) but not for common-garden or quantitative genetic studies (which minimize plastic effects). We also find that phenotypic changes in response to disturbance can be remarkably abrupt, perhaps again because of plasticity. In short, humans are an important agent driving phenotypic change in contemporary populations. Although these changes sometimes have a genetic basis, our analyses suggest a particularly important contribution from phenotypic plasticity.
- Published
- 2008
- Full Text
- View/download PDF
7. Evolutionary responses to climate change.
- Author
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Skelly DK, Joseph LN, Possingham HP, Freidenburg LK, Farrugia TJ, Kinnison MT, and Hendry AP
- Subjects
- Conservation of Natural Resources, Biological Evolution, Greenhouse Effect
- Published
- 2007
- Full Text
- View/download PDF
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