1. Molecular cloning and expression of a delta-opioid receptor from rat brain.
- Author
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Abood ME, Noel MA, Farnsworth JS, and Tao Q
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Blotting, Northern, Cells, Cultured, Cloning, Molecular, DNA Probes, DNA, Complementary biosynthesis, Diprenorphine pharmacokinetics, Gene Library, Molecular Sequence Data, Olfactory Bulb metabolism, RNA analysis, Rats, Rats, Sprague-Dawley, Receptors, Opioid, delta genetics, Transfection, Brain Chemistry physiology, Receptors, Opioid, delta biosynthesis
- Abstract
We have isolated and characterized a rat delta-opioid receptor. The deduced amino acid sequence (372 aa) closely resembles the murine delta-opioid receptor, DOR-1. In fact, 97% of the amino acid residues are conserved between the two species, while 93% of the nucleic acid residues are identical. A 6 kb mRNA was detected in rat cortex using rat DOR-1 as a probe. When expressed in COS cells, the clone shows high-affinity opioid binding with selectivity for delta-opioids. The rat delta-opioid receptor cDNA clone will be a useful tool for studying the function of delta-opioid receptor in rats.
- Published
- 1994
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