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1. A novel aqueous micellar two-phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme fromDurio zibethinus Murrayand recycling phase components

Author

Farhana Azmira Asmadi, Md. Zaidul Islam Sarker, Muhaini Hussin, Mohd Yazid Abd Manap, Mehrnoush Amid, and Chong Gun Hean

Subjects
Chromatography, Aqueous solution, 010405 organic chemistry, Chemistry, Process Chemistry and Technology, General Chemical Engineering, 010401 analytical chemistry, Filtration and Separation, General Chemistry, 01 natural sciences, 0104 chemical sciences, law.invention, Magazine, Pulmonary surfactant, law, Phase (matter), Yield (chemistry), medicine, Composition (visual arts), Mannitol, Pectinase, medicine.drug
Abstract

A novel aqueous micellar two-phase system composed of surfactant and mannitol was employed for the first time to purify polygalacturonase from Durio zibethinus Murray. The influences of different parameters such as type of surfactants, composition of surfactant/mannitol, pH and crude load on polygalacturonase partitioning were investigated. The results indicated that the polygalacturonase was recovered in 25% Tween 80 and 22% mannitol, at 68.2% of Tie line length, crude load of 30% at pH 5.0. The enzyme was successfully recovered with a high purification factor of 16.1 and yield of 97.3% while phase components were also recovered and recycled above 95%.

Published
2016

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