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12. Extracellular Vesicles from Fusarium graminearum Contain Protein Effectors Expressed during Infection of Corn

Author

Garcia-Ceron, Donovan, primary, Lowe, Rohan G. T., additional, McKenna, James A., additional, Brain, Linda M., additional, Dawson, Charlotte S., additional, Clark, Bethany, additional, Berkowitz, Oliver, additional, Faou, Pierre, additional, Whelan, James, additional, Bleackley, Mark R., additional, and Anderson, Marilyn A., additional

Published
2021
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14. Divergent features of the coenzyme Q: Cytochrome c oxidoreductase complex in Toxoplasma gondii parasites

Author

JA Hayward, E Rajendran, SM Zwahlen, Faou, Pierre, and GG Van Dooren

Subjects
parasitic diseases, Uncategorized
Abstract

© 2021 Hayward et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The mitochondrion is critical for the survival of apicomplexan parasites. Several major antiparasitic drugs, such as atovaquone and endochin-like quinolones, act through inhibition of the mitochondrial electron transport chain at the coenzyme Q:cytochrome c oxidoreductase complex (Complex III). Despite being an important drug target, the protein composition of Complex III of apicomplexan parasites has not been elucidated. Here, we undertake a mass spectrometry-based proteomic analysis of Complex III in the apicomplexan Toxoplasma gondii. Along with canonical subunits that are conserved across eukaryotic evolution, we identify several novel or highly divergent Complex III components that are conserved within the apicomplexan lineage. We demonstrate that one such subunit, which we term TgQCR11, is critical for parasite proliferation, mitochondrial oxygen consumption and Complex III activity, and establish that loss of this protein leads to defects in Complex III integrity. We conclude that the protein composition of Complex III in apicomplexans differs from that of the mammalian hosts that these parasites infect.

Published
2021
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17. Solvent interactions of halophilic malate dehydrogenase

Author

Ebel, Christine, Costenaro, Lionel, Pascu, Mihaela, Faou, Pierre, Kernel, Blandine, Martin, Flavien Proust-De, and Zaccai, Giuseppe

Subjects
Biochemistry -- Research, Oxidoreductases -- Physiological aspects, Solvents, Salts, Acids, Proteins -- Physiological aspects, Biological sciences, Chemistry
Abstract

Research has been conducted on acidic protein, malate dehydrogenase from halophilic Haloarcula marismortui which is unstable below molar salt concentrations. The study of the solvated foded protein carried out via small-angle neutron scattering in solvents containing salts is described.

Published
2002

21. Longitudinal Stroke Recovery Associated With Dysregulation of Complement System—A Proteomics Pathway Analysis

Author

Nguyen, Vinh A., primary, Riddell, Nina, additional, Crewther, Sheila G., additional, Faou, Pierre, additional, Rajapaksha, Harinda, additional, Howells, David W., additional, Hankey, Graeme J., additional, Wijeratne, Tissa, additional, Ma, Henry, additional, Davis, Stephen, additional, Donnan, Geoffrey A., additional, and Carey, Leeanne M., additional

Published
2020
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24. Relative role of anions and cations in the stabilization of halophilic malate dehydrogenase

Author

Ebel, Christine, Faou, Pierre, Kernel, Blandine, and Zaccai, Guiseppe

Subjects
Anions -- Physiological aspects, Cations -- Physiological aspects, Enzymes -- Structure-activity relationship, Proteins -- Conformation, Biological sciences, Chemistry
Abstract

Results indicate that high charge density anions and cations efficiently stabilize folded form of the enzyme but the stabilizing effect of the latter is influenced by the salt concentration, higher concentration denaturing the enzyme.

Published
1999

25. Influenza A virus infection induces viral and cellular defective ribosomal products encoded by alternative reading frames

Author

Zanker, Damien J, Oveissi, Sara, Tscharke, David C, Duan, Mubing, Wan, Siyuan, Zhang, Xiaomu, Xiao, Kun, Mifsud, Nicole A, Gibbs, James, Izzard, Lenny, Dlugolenski, Daniel, Faou, Pierre, Laurie, Karen L, Vigneron, Nathalie, Barr, Ian G, Stambas, John, Van den Eynde, Benoit J, Bennink, Jack R, Yewdell, Jonathan W, Chen, Weisan, Zanker, Damien J, Oveissi, Sara, Tscharke, David C, Duan, Mubing, Wan, Siyuan, Zhang, Xiaomu, Xiao, Kun, Mifsud, Nicole A, Gibbs, James, Izzard, Lenny, Dlugolenski, Daniel, Faou, Pierre, Laurie, Karen L, Vigneron, Nathalie, Barr, Ian G, Stambas, John, Van den Eynde, Benoit J, Bennink, Jack R, Yewdell, Jonathan W, and Chen, Weisan

Published
2019

30. Influenza A Virus Infection Induces Viral and Cellular Defective Ribosomal Products Encoded by Alternative Reading Frames

Author

Zanker, Damien J., primary, Oveissi, Sara, additional, Tscharke, David C., additional, Duan, Mubing, additional, Wan, Siyuan, additional, Zhang, Xiaomu, additional, Xiao, Kun, additional, Mifsud, Nicole A., additional, Gibbs, James, additional, Izzard, Lenny, additional, Dlugolenski, Daniel, additional, Faou, Pierre, additional, Laurie, Karen L., additional, Vigneron, Nathalie, additional, Barr, Ian G., additional, Stambas, John, additional, Van den Eynde, Benoît J., additional, Bennink, Jack R., additional, Yewdell, Jonathan W., additional, and Chen, Weisan, additional

Published
2019
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33. Circulating BiP/Grp78 is a novel prognostic marker for sepsis‐mediated immune cell death.

Author

Doerflinger, Marcel, Reljic, Boris, Menassa, Joseph, Nedeva, Christina, Jose, Irvin, Faou, Pierre, Mackiewicz, Liana, Mansell, Ashley, Pellegrini, Marc, Hotchkiss, Richard, and Puthalakath, Hamsa

Subjects
CELL death, BIOMARKERS, PROGNOSIS, IMMUNOSUPPRESSION, ENDOPLASMIC reticulum, SEPSIS
Abstract

Sepsis remains to be a major contributor to mortality in ICUs, and immune suppression caused by immune cell apoptosis determines the overall patient survival. However, diagnosis of sepsis‐induced lymphopenia remains problematic with no accurate prognostic techniques or biomarkers for cell death available. Developing reliable prognostic tools for sepsis‐mediated cell death is not only important for identifying patients at increased risk of immune suppression but also to monitor treatment progress of currently trialed immunotherapy strategies. We have previously shown an important role for endoplasmic reticulum stress (ER stress) in inducing sepsis‐mediated cell death and here report on the identification of a secreted form of the ER chaperone BiP (immunoglobulin binding protein) as a novel circulating prognostic biomarker for immune cell death and ER stress during sepsis. Using biochemical purification and mass spectrometry coupled with an established in vitro sepsis cell death assay, we identified BiP/Grp78 as a factor secreted by lipopolysaccharide‐activated macrophages that is capable of inducing cell death in target cells. Quantitative ELISA analysis showed significantly elevated levels of circulating BiP in mice undergoing polymicrobial sepsis, which was absent in Bim−/− mice that are protected from sepsis‐induced lymphopenia. Using blood serum from human sepsis patients, we could detect a significant difference in levels of secreted BiP in sepsis patients compared to nonseptic controls, suggesting that secreted circulating BiP could indeed be used as a prognostic marker that is directly correlative to immune cell death during sepsis. [ABSTRACT FROM AUTHOR]

Published
2021
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34. LMP2 immunoproteasome promotes lymphocyte survival by degrading apoptotic BH3-only proteins

Author

Zanker, Damien, Pang, Kenneth, Oveissi, Sara, Lu, Chunni, Faou, Pierre, Nowell, Cameron, Mbogo, George W, Carotta, Sebastian, Quillici, Cathy, Karupiah, Guna, Hibbs, Margaret L, Nutt, Stephen L, Neeson, Paul, Puthalakath, Hamsa, Chen, Weisan, Zanker, Damien, Pang, Kenneth, Oveissi, Sara, Lu, Chunni, Faou, Pierre, Nowell, Cameron, Mbogo, George W, Carotta, Sebastian, Quillici, Cathy, Karupiah, Guna, Hibbs, Margaret L, Nutt, Stephen L, Neeson, Paul, Puthalakath, Hamsa, and Chen, Weisan

Published
2018

35. Elucidating the mitochondrial proteome of Toxoplasma gondii reveals the presence of a divergent cytochrome c oxidase

Author

Seidi, Azadeh, Muellner-Wong, Linden, Rajendran, Esther, Tjhin, Edwin, Dagley, Laura F, Vincent, Aw, Faou, Pierre, Webb, Andrew I., Tonkin, Christopher J., van Dooren, Giel, Seidi, Azadeh, Muellner-Wong, Linden, Rajendran, Esther, Tjhin, Edwin, Dagley, Laura F, Vincent, Aw, Faou, Pierre, Webb, Andrew I., Tonkin, Christopher J., and van Dooren, Giel

Abstract

The mitochondrion of apicomplexan parasites is critical for parasite survival, although the full complement of proteins that localize to this organelle has not been defined. Here we undertake two independent approaches to elucidate the mitochondrial proteome of the apicomplexan Toxoplasma gondii. We identify approximately 400 mitochondrial proteins, many of which lack homologs in the animals that these parasites infect, and most of which are important for parasite growth. We demonstrate that one such protein, termed TgApiCox25, is an important component of the parasite cytochrome c oxidase (COX) complex. We identify numerous other apicomplexan-specific components of COX, and conclude that apicomplexan COX, and apicomplexan mitochondria more generally, differ substantially in their protein composition from the hosts they infect. Our study highlights the diversity that exists in mitochondrial proteomes across the eukaryotic domain of life, and provides a foundation for defining unique aspects of mitochondrial biology in an important phylum of parasites.

Published
2018

36. Shotgun Proteomics Analysis of Saliva and Salivary Gland Tissue from the Common Octopus Octopus vulgaris

Author

Fingerhut, Legana C. H., Strugnell, Jan M., Faou, Pierre, Roura, Álvaro, Zhang, Jia, Cooke, Ira R., Fingerhut, Legana C. H., Strugnell, Jan M., Faou, Pierre, Roura, Álvaro, Zhang, Jia, and Cooke, Ira R.

Abstract

The salivary apparatus of the common octopus (Octopus vulgaris) has been the subject of biochemical study for over a century. A combination of bioassays, behavioral studies and molecular analysis on O. vulgaris and related species suggests that its proteome should contain a mixture of highly potent neurotoxins and degradative proteins. However, a lack of genomic and transcriptomic data has meant that the amino acid sequences of these proteins remain almost entirely unknown. To address this, we assembled the posterior salivary gland transcriptome of O. vulgaris and combined it with high resolution mass spectrometry data from the posterior and anterior salivary glands of two adults, the posterior salivary glands of six paralarvae and the saliva from a single adult. We identified a total of 2810 protein groups from across this range of salivary tissues and age classes, including 84 with homology to known venom protein families. Additionally, we found 21 short secreted cysteine rich protein groups of which 12 were specific to cephalopods. By combining protein expression data with phylogenetic analysis we demonstrate that serine proteases expanded dramatically within the cephalopod lineage and that cephalopod specific proteins are strongly associated with the salivary apparatus

Published
2018

40. LMP2 immunoproteasome promotes lymphocyte survival by degrading apoptotic BH3‐only proteins

Author

Zanker, Damien, primary, Pang, Kenneth, additional, Oveissi, Sara, additional, Lu, Chunni, additional, Faou, Pierre, additional, Nowell, Cameron, additional, Mbogo, George W, additional, Carotta, Sebastian, additional, Quillici, Cathy, additional, Karupiah, Guna, additional, Hibbs, Margaret L., additional, Nutt, Stephen L, additional, Neeson, Paul, additional, Puthalakath, Hamsa, additional, and Chen, Weisan, additional

Published
2018
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49. A Pathway Proteomic Profile of Ischemic Stroke Survivors Reveals Innate Immune Dysfunction in Association with Mild Symptoms of Depression – A Pilot Study

Author

Nguyen, Vinh A., primary, Carey, Leeanne M., additional, Giummarra, Loretta, additional, Faou, Pierre, additional, Cooke, Ira, additional, Howells, David W., additional, Tse, Tamara, additional, Macaulay, S. Lance, additional, Ma, Henry, additional, Davis, Stephen M., additional, Donnan, Geoffrey A., additional, and Crewther, Sheila G., additional

Published
2016
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50. The recently identified modifier of murine metastable epialleles, Rearranged L-Myc Fusion, is involved in maintaining epigenetic marks at CpG island shores and enhancers

Author

Harten, Sarah, Oey, Harald, Bourke, Lauren, Vandhana, Bharti, Isbel, Luke, Daxinger, Lucia, Faou, Pierre, Robertson, Neil, Matthews, Jacqueline, Whitelaw, Emma, Harten, Sarah, Oey, Harald, Bourke, Lauren, Vandhana, Bharti, Isbel, Luke, Daxinger, Lucia, Faou, Pierre, Robertson, Neil, Matthews, Jacqueline, and Whitelaw, Emma

Abstract

Background - We recently identified a novel protein, Rearranged L-myc fusion (Rlf), that is required for DNA hypomethylation and transcriptional activity at two specific regions of the genome known to be sensitive to epigenetic gene silencing. To identify other loci affected by the absence of Rlf, we have now analysed 12 whole genome bisulphite sequencing datasets across three different embryonic tissues/stages from mice wild-type or null for Rlf. Results - Here we show that the absence of Rlf results in an increase in DNA methylation at thousands of elements involved in transcriptional regulation and many of the changes occur at enhancers and CpG island shores. ChIP-seq for H3K4me1, a mark generally found at regulatory elements, revealed associated changes at many of the regions that are differentially methylated in the Rlf mutants. RNA-seq showed that the numerous effects of the absence of Rlf on the epigenome are associated with relatively subtle effects on the mRNA population. In vitro studies suggest that Rlf’s zinc fingers have the capacity to bind DNA and that the protein interacts with other known epigenetic modifiers. Conclusion - This study provides the first evidence that the epigenetic modifier Rlf is involved in the maintenance of DNA methylation at enhancers and CGI shores across the genome.

Published
2015

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