36 results on '"Fantini V"'
Search Results
2. 2,5 KW CO2 CW Modular Laser for Industrial Applications
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Fantini, V., Garifo, L., Incerti, G., Cerri, W., and Waidelich, Wilhelm, editor
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- 1986
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3. Flow Effects on the Laser Power of Transverse Flow TE Lasers
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Fantini, V., Donati, V., Garifo, L., Incerti, G., Borghese, A., and Onorato, Michele, editor
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- 1984
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4. IL CARCINOMA PROSTATICO NELLE GHIANDOLE DI GRANDI DIMENSIONI SONO ASSOCIATE AD UN MINOR TASSO DI UPGRADING TRA BIOPSIA E PROSTATECTOMIA RADICALE
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Gallina A, Walz J, Trinh Quoc Dien, Scattoni V, Eastham JA, Zanni G, Gemma, Fantini V, Suardi N, Graefen M, Huland H, Karakiewicz PI, Rigatti P, MONTORSI, FRANCESCO, BRIGANTI , ALBERTO, Gallina, A, Briganti, Alberto, Walz, J, Trinh Quoc, Dien, Scattoni, V, Eastham, Ja, Zanni, G, Gemma, Fantini, V, Suardi, N, Graefen, M, Huland, H, Karakiewicz, Pi, Rigatti, P, and Montorsi, Francesco
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- 2007
5. Disfunzioni sessuali in donne affette da cardiopatia ischemica
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SALONIA, ANDREA, Montorsi P, MARGONATO, ALBERTO, Longhi E, Nappi R, Gemma E, Fantini V, Fabbri F, Rigatti P, MONTORSI, FRANCESCO, BRIGANTI , ALBERTO, Salonia, Andrea, Briganti, Alberto, Montorsi, P, Margonato, Alberto, Longhi, E, Nappi, R, Gemma, E, Fantini, V, Fabbri, F, Rigatti, P, and Montorsi, Francesco
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- 2004
6. Advancements in RDF Co-firing Demonstration Project at Enel Fusina Power Plant
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Lattanzi, S., Rossi, N., La Marca, C., Gasperetti, S., Dalle Mura, D., Molina, G., Fantini, V., and Cavaliere, A.
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Biomass - Abstract
ENEL is carrying out a demonstration project at Fusina Power Plant, aimed at proving the feasibility of high share RDF (Refuse Derived Fuel)/coal co-firing. Since 2004, RDF has been co-fired with coal on two 320 MWe boilers (units 3 and 4). From February 2009 the average share of co-firing has been increased up to 5% on thermal input. Fusina power plant is involved in the European Founded DEBCO (Demonstration of Biomass Co-combustion) project focused on the demonstration of large scale biomass co-firing and supply chain integration. In this framework long term monitoring of RDF quality and boiler performance is foreseen. Since October 2009 corrosion monitoring probes have been installed, both on boiler walls and convective pass. In order to monitor corrosion behaviour also in ultra-super-critical (USC) conditions, corrosion probes with advanced materials will be installed. Performances of main air pollution control devices (APCD) have been monitored as well, through an extended experimental measurement campaign carried out in October 2009. In this paper the ongoing activities are described including secondary fuel quality and milling performance monitoring, corrosion evaluation, slagging and flue gas cleaning system performance assessment. The aim of EU project is to demonstrate that RDF can be co-fired safely and with low impact on plant performance and will take to a general optimization of the fuel supply system and of the combustion process., Proceedings of the 18th European Biomass Conference and Exhibition, 3-7 May 2010, Lyon, France, pp. 1078-1084
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- 2010
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7. SL 25, A NEW HIGH PERFORMANCE 2.5 KW INDUSTRIAL CO2 LASER
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Fantini, V., primary and Incerti, G., additional
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- 1989
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8. PERCORSI RETURN E TRAVERSAL IN UN SISTEMA DI PICKING
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Fantini, V and Spirito, Francesco
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- 2005
9. Rivestimenti anticorrosione per serpentini SH di inceneritori
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Quaglia, G.M. and Fantini, V.
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- 2004
10. The “Terme Del Corallo” in Livorno, Italy, a Contribution to Try Going beyond the Abandon
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Verdiani, G., primary and Fantini, V., additional
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- 2012
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11. Laser Cladding: A New Technology for Corrosion and Erosion Protection of Boiler Tubes
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Fantini, V., additional
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- 2007
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12. Laser surface treatment of materials by using CO 2 laser beam at oblique incidence
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Taca, M., primary, Alexandrescu, E., additional, Fantini, V., additional, Serri, L., additional, Ivanov, I., additional, Petre, C., additional, Mihailescu, Ion N., additional, and Gutu, Iulian, additional
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- 2001
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13. Laser surface treatment of materials by using CO2 laser beam at oblique incidence.
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Taca, M., Alexandrescu, E., Fantini, V., Serri, L., Ivanov, I., Petre, C., Mihailescu, Ion N., and Gutu, Iulian
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- 2001
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14. Use of resonators with graded reflectivity mirrors in transverse flow CO2laser sources
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Fantini, V, primary, Galletti, E, additional, Garifo, L, additional, Serri, L, additional, Stucchi, E, additional, Magni, V, additional, Silvestri, S De, additional, and Svelto, O, additional
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- 1994
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15. High homogeneity gain distribution in a cross flow AC excited CO/sub 2/ laser
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Burlamacchi, P., primary, Congiu, F., additional, Fantini, V., additional, Salimbeni, R., additional, and Vanni, P., additional
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- 1990
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16. Use of resonators with graded reflectivity mirrors in transverse flow CO2 laser sources.
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Fantini, V, Galletti, E, Garifo, L, Serri, L, Stucchi, E, Magni, V, Silvestri, S De, and Svelto, O
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- 1994
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17. Transverse flow cw 5-kW CO2 laser
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Fantini, V., primary, Incerti, G., additional, Pandarese, F., additional, and Garifo, L., additional
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- 1984
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18. High performance 2.5 kW industrial CO 2 laser
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Fantini, V, primary and Incerti, G, additional
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- 1986
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19. Oltre De Martino. Per una rifondazione degli studi sul tarantismo
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SALVATORE, Gianfranco, P. Apolito, G. L. Di Mitri, B. Fantini, V. Lanternari, G. Mina, M. R. Tamblé, G. Salvatore, and Salvatore, Gianfranco
- Subjects
Ernesto De Martino ,Tarantismo ,Antropologia della musica e della danza ,Storia delle religioni - Abstract
Il saggio torna sul classico demartiniano La terra del rimorso per riconsiderare l’idea della dimensione mitico-rituale del tarantismo, legata alla definizione di aspetti simbolici “culturalmente condizionati”, che rappresentavano una delle novità metodologiche dell’opera, accanto a quei paralleli etno-folklorici e confronti con gli antecedenti classici già introdotti nelle opere precedenti (in particolare in Morte e pianto rituale). Viene criticata la definizione di “relitti”, “rottami”, “macerie” che per de Martino rappresenterebbero nel tarantismo solo una pallida eco di antichi riti greci, legati in particolare al menadismo dionisiaco e alla sfera coribantica: pur ammettendo una «comune realtà religiosa», lo studioso rinunciava a tracciare la storia «della civiltà religiosa di cui [il tarantismo] è relitto», considerando il fenomeno come una «nuova plasmazione irriducibile ad altro». Ma il confronto col tarantismo campano, poco conosciuto e studiato, e con analoghe tradizioni non solo sarde ma anche calabresi e siciliane, corredato anche da nuove testimonianze sul tarantismo raccolte successivamente al lavoro demartiniano, consente di completare il quadro antropologico riconoscendovi elementi che conducono verso conclusioni in parte diverse da quelle dello studioso napoletano. In particolare si riscontra che paraphernalia e altri aspetti del rituale, che de Martino considerava secondari, godevano invece di piena autonomia simbolica nel tarantismo, nel quale inoltre sussisteva - come emerge ad esempio in certa narrativa salentina di tradizioni mariana - una concezione demonica della taranta non dissimile da analoghi aspetti del dionisismo. La nuova analisi comparativa offre così un sistema di costanti e varianti che, mentre da una parte riconduce il tarantismo alla fenomenologia generale della trance religiosa, evidenzia dall’altra l’emergenza di tematiche e problematiche già pienamente appartenenti al mondo classico, come l’interpretazione demonica della sindrome malinconica e del “colpo di sole”, e gli aspetti non olimpici ma demonici del dionisismo. In un quadro più completo e meglio contestualizzato, la stessa figura di san Paolo come nume tutelare - anch’egli colpito nella carne da un pungolo o pungiglione (Seconda Lettera ai Corinti 12, 7, e Atti degli Apostoli 9, 6, con precedenti veterotestamentari), in una prospettiva comune all’oistros dionisiaco e orfico ma scartata da de Martino - appare non tanto imposta dal clero ma coerente con un processo di cristianizzazione di antiche credenze demoniche, tipologicamente autonomo dalle autorità cattoliche e ben rappresentato nella storia della religiosità popolare e dei suoi sincretismi.
- Published
- 2000
20. Effective lowering of α-synuclein expression by targeting G-quadruplex structures within the SNCA gene.
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Pirota V, Rey F, Esposito L, Fantini V, Pandini C, Maghraby E, Di Gerlando R, Doria F, Mella M, Pansarasa O, Gandellini P, Freccero M, Carelli S, and Cereda C
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- Humans, RNA, Messenger genetics, RNA, Messenger metabolism, Cell Line, Tumor, Promoter Regions, Genetic, Gene Expression Regulation drug effects, 5' Untranslated Regions genetics, Parkinson Disease genetics, Parkinson Disease metabolism, Peptide Nucleic Acids pharmacology, Peptide Nucleic Acids chemistry, alpha-Synuclein genetics, alpha-Synuclein metabolism, G-Quadruplexes
- Abstract
Alpha-synuclein, encoded by the SNCA gene, is a pivotal protein implicated in the pathogenesis of synucleinopathies, including Parkinson's disease. Current approaches for modulating alpha-synuclein levels involve antisense nucleotides, siRNAs, and small molecules targeting SNCA's 5'-UTR mRNA. Here, we propose a groundbreaking strategy targeting G-quadruplex structures to effectively modulate SNCA gene expression and lowering alpha-synuclein amount. Novel G-quadruplex sequences, identified on the SNCA gene's transcription starting site and 5'-UTR of SNCA mRNAs, were experimentally confirmed for their stability through biophysical assays and in vitro experiments on human genomic DNA. Biological validation in differentiated SH-SY5Y cells revealed that well-known G-quadruplex ligands remarkably stabilized these structures, inducing the modulation of SNCA mRNAs expression, and the effective decrease in alpha-synuclein amount. Besides, a novel peptide nucleic acid conjugate, designed to selectively disrupt of G-quadruplex within the SNCA gene promoter, caused a promising lowering of both SNCA mRNA and alpha-synuclein protein. Altogether our findings highlight G-quadruplexes' key role as intriguing biological targets in achieving a notable and successful reduction in alpha-synuclein expression, pointing to a novel approach against synucleinopathies., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
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- 2024
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21. Functional analysis and transcriptome profile of meninges and skin fibroblasts from human-aged donors.
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Fantini V, Ferrari RR, Bordoni M, Spampinato E, Pandini C, Davin A, Medici V, Gagliardi S, Guaita A, Pansarasa O, Cereda C, and Poloni TE
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- Humans, Gene Expression Profiling, Aged, Cells, Cultured, Nestin metabolism, Nestin genetics, Cyclic AMP metabolism, Middle Aged, Female, Male, Colforsin pharmacology, Fibroblasts metabolism, Fibroblasts cytology, Skin metabolism, Skin cytology, Meninges metabolism, Meninges cytology, Transcriptome
- Abstract
The central nervous system (CNS) is surrounded by three membranes called meninges. Specialised fibroblasts, originating from the mesoderm and neural crest, primarily populate the meninges and serve as a binding agent. Our goal was to compare fibroblasts from meninges and skin obtained from the same human-aged donors, exploring their molecular and cellular characteristics related to CNS functions. We isolated meningeal fibroblasts (MFs) from brain donors and skin fibroblasts (SFs) from the same subjects. A functional analysis was performed measuring cell appearance, metabolic activity, and cellular orientation. We examined fibronectin, serpin H1, β-III-tubulin, and nestin through qPCR and immunofluorescence. A whole transcriptome analysis was also performed to characterise the gene expression of MFs and SFs. MFs appeared more rapidly in the post-tissue processing, while SFs showed an elevated cellular metabolism and a well-defined cellular orientation. The four markers were mostly similar between the MFs and SFs, except for nestin, more expressed in MFs. Transcriptome analysis reveals significant differences, particularly in cyclic adenosine monophosphate (cAMP) metabolism and response to forskolin, both of which are upregulated in MFs. This study highlights MFs' unique characteristics, including the timing of appearance, metabolic activity, and gene expression patterns, particularly in cAMP metabolism and response to forskolin. These findings contribute to a deeper understanding of non-neuronal cells' involvement in CNS activities and potentially open avenues for therapeutic exploration., (© 2024 The Authors. Cell Proliferation published by Beijing Institute for Stem Cell and Regenerative Medicine and John Wiley & Sons Ltd.)
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- 2024
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22. The long non-coding RNA TAZ-AS202 promotes lung cancer progression via regulation of the E2F1 transcription factor and activation of Ephrin signaling.
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Gobbi G, Grieco A, Torricelli F, Sauta E, Santandrea G, Zanetti E, Fantini V, Reggiani F, Strocchi S, Paci M, Vohra M, Saladi SV, Ambrosetti DC, Ciarrocchi A, and Sancisi V
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- Humans, E2F1 Transcription Factor genetics, E2F1 Transcription Factor metabolism, Ephrins genetics, Ephrins metabolism, Cell Line, Tumor, Lung metabolism, Gene Expression Regulation, Neoplastic genetics, Lung Neoplasms pathology, RNA, Long Noncoding genetics, RNA, Long Noncoding metabolism, Carcinoma, Non-Small-Cell Lung genetics
- Abstract
Long non-coding RNAs (lncRNAs) are transcripts without coding potential that are pervasively expressed from the genome and have been increasingly reported to play crucial roles in all aspects of cell biology. They have been also heavily implicated in cancer development and progression, with both oncogenic and tumor suppressor functions. In this work, we identified and characterized a novel lncRNA, TAZ-AS202, expressed from the TAZ genomic locus and exerting pro-oncogenic functions in non-small cell lung cancer. TAZ-AS202 expression is under the control of YAP/TAZ-containing transcriptional complexes. We demonstrated that TAZ-AS202 is overexpressed in lung cancer tissue, compared with surrounding lung epithelium. In lung cancer cell lines TAZ-AS202 promotes cell migration and cell invasion. TAZ-AS202 regulates the expression of a set of genes belonging to cancer-associated pathways, including WNT and EPH-Ephrin signaling. The molecular mechanism underlying TAZ-AS202 function does not involve change of TAZ expression or activity, but increases the protein level of the transcription factor E2F1, which in turn regulates the expression of a large set of target genes, including the EPHB2 receptor. Notably, the silencing of both E2F1 and EPHB2 recapitulates TAZ-AS202 silencing cellular phenotype, indicating that they are essential mediators of its activity. Overall, this work unveiled a new regulatory mechanism that, by increasing E2F1 protein, modifies the non-small cell lung cancer cells transcriptional program, leading to enhanced aggressiveness features. The TAZ-AS202/E2F1/EPHB2 axis may be the target for new therapeutic strategies., (© 2023. The Author(s).)
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- 2023
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23. The expression pattern of GDF15 in human brain changes during aging and in Alzheimer's disease.
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Chiariello A, Valente S, Pasquinelli G, Baracca A, Sgarbi G, Solaini G, Medici V, Fantini V, Poloni TE, Tognocchi M, Arcaro M, Galimberti D, Franceschi C, Capri M, Salvioli S, and Conte M
- Abstract
Introduction: Growth Differentiation Factor 15 (GDF15) is a mitochondrial-stress-responsive molecule whose expression strongly increases with aging and age-related diseases. However, its role in neurodegenerative diseases, including Alzheimer's disease (AD), is still debated., Methods: We have characterized the expression of GDF15 in brain samples from AD patients and non-demented subjects (controls) of different ages., Results: Although no difference in CSF levels of GDF15 was found between AD patients and controls, GDF15 was expressed in different brain areas and seems to be predominantly localized in neurons. The ratio between its mature and precursor form was higher in the frontal cortex of AD patients compared to age-matched controls ( p < 0.05). Moreover, this ratio was even higher for centenarians ( p < 0.01), indicating that aging also affects GDF15 expression and maturation. A lower expression of OXPHOS complexes I, III, and V in AD patients compared to controls was also noticed, and a positive correlation between GDF15 and IL-6 mRNA levels was observed. Finally, when GDF15 was silenced in vitro in dermal fibroblasts, a decrease in OXPHOS complexes transcript levels and an increase in IL-6 levels were observed., Discussion: Although GDF15 seems not to be a reliable CSF marker for AD, it is highly expressed in aging and AD brains, likely as a part of stress response aimed at counteracting mitochondrial dysfunction and neuroinflammation., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Chiariello, Valente, Pasquinelli, Baracca, Sgarbi, Solaini, Medici, Fantini, Poloni, Tognocchi, Arcaro, Galimberti, Franceschi, Capri, Salvioli and Conte.)
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- 2023
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24. cDCD organ donation pathway of Romagna Local Health Authority: strategic planning, organizational management, and results.
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Circelli A, Antonini MV, Nanni A, Prugnoli M, Gamberini E, Maitan S, Gecele C, Viola L, Bissoni L, Scognamiglio G, Mezzatesta L, Bergamini C, Gobbi L, Meca MCC, Sangiorgi G, Bisulli M, Spiga M, Pransani V, Liuzzi D, Fantini V, Catena F, Russo E, and Agnoletti V
- Abstract
The introduction of pathways to enrol deceased donors after cardio-circulatory confirmation of death (donation after circulatory death, DCD) is expanding in many countries to face the shortage of organs for transplantation. The implementation of normothermic regional reperfusion (NRP) with warm oxygenated blood is a strategy to manage in-situ the organs of DCD donors. This approach, an alternative to in-situ cold preservation, and followed by prompt retrieval and cold static storage and/or ex-vivo machine perfusion (EVMP), could be limited to abdominal organs (A-NRP) or extended to the thorax (thoraco-abdominal, TA-NRP. NRP is also referred to as extracorporeal interval support for organ retrieval (EISOR). The use of EISOR is increasing in Europe, even if variably regulated. A-NRP has been demonstrated to be effective in decreasing the risk associated with transplantation of abdominal organs from DCD donors, and was recommended by the European Society for Organ Transplantation (ESOT) in a recent consensus document. We aim to explain how we select the candidates for DCD, to describe our regionalized model for implementing EISOR provision, and to introduce the health care professionals involved in this complex process, with their strictly defined roles, responsibilities, and boundaries. Finally, we report the results of our program, recruiting cDCD donors over a large network of hospitals, all pertaining to a Local Health Authority (Azienda Unità Sanitaria Locale, AUSL) in Romagna, Italy., Competing Interests: Competing interestsThe authors declare no competing interests., (© The Author(s) 2023.)
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- 2023
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25. COVID-19 Pathology in the Lung, Kidney, Heart and Brain: The Different Roles of T-Cells, Macrophages, and Microthrombosis.
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Poloni TE, Moretti M, Medici V, Turturici E, Belli G, Cavriani E, Visonà SD, Rossi M, Fantini V, Ferrari RR, Carlos AF, Gagliardi S, Tronconi L, Guaita A, and Ceroni M
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- Aged, Antigens, Viral, Brain pathology, Humans, Kidney, Lung pathology, Macrophages, RNA, Viral, SARS-CoV-2, T-Lymphocytes, COVID-19, Thrombosis pathology
- Abstract
Here, we aim to describe COVID-19 pathology across different tissues to clarify the disease's pathophysiology. Lungs, kidneys, hearts, and brains from nine COVID-19 autopsies were compared by using antibodies against SARS-CoV-2, macrophages-microglia, T-lymphocytes, B-lymphocytes, and activated platelets. Alzheimer's Disease pathology was also assessed. PCR techniques were used to verify the presence of viral RNA. COVID-19 cases had a short clinical course (0-32 days) and their mean age was 77.4 y/o. Hypoxic changes and inflammatory infiltrates were present across all tissues. The lymphocytic component in the lungs and kidneys was predominant over that of other tissues ( p < 0.001), with a significantly greater presence of T-lymphocytes in the lungs ( p = 0.020), which showed the greatest presence of viral antigens. The heart showed scant SARS-CoV-2 traces in the endothelium-endocardium, foci of activated macrophages, and rare lymphocytes. The brain showed scarce SARS-CoV-2 traces, prominent microglial activation, and rare lymphocytes. The pons exhibited the highest microglial activation ( p = 0.017). Microthrombosis was significantly higher in COVID-19 lungs ( p = 0.023) compared with controls. The most characteristic pathological features of COVID-19 were an abundance of T-lymphocytes and microthrombosis in the lung and relevant microglial hyperactivation in the brainstem. This study suggests that the long-term sequelae of COVID-19 derive from persistent inflammation, rather than persistent viral replication.
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- 2022
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26. Patients' Stem Cells Differentiation in a 3D Environment as a Promising Experimental Tool for the Study of Amyotrophic Lateral Sclerosis.
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Scarian E, Bordoni M, Fantini V, Jacchetti E, Raimondi MT, Diamanti L, Carelli S, Cereda C, and Pansarasa O
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- Humans, Hydrogels pharmacology, Leukocytes, Mononuclear pathology, Amyotrophic Lateral Sclerosis pathology, Induced Pluripotent Stem Cells, Neurodegenerative Diseases
- Abstract
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease (NDD) that affects motor neurons, causing weakness, muscle atrophy and spasticity. Unfortunately, there are only symptomatic treatments available. Two important innovations in recent years are three-dimensional (3D) bioprinting and induced pluripotent stem cells (iPSCs). The aim of this work was to demonstrate the robustness of 3D cultures for the differentiation of stem cells for the study of ALS. We reprogrammed healthy and sALS peripheral blood mononuclear cells (PBMCs) in iPSCs and differentiated them in neural stem cells (NSCs) in 2D. NSCs were printed in 3D hydrogel-based constructs and subsequently differentiated first in motor neuron progenitors and finally in motor neurons. Every step of differentiation was tested for cell viability and characterized by confocal microscopy and RT-qPCR. Finally, we tested the electrophysiological characteristics of included NSC34. We found that NSCs maintained good viability during the 3D differentiation. Our results suggest that the hydrogel does not interfere with the correct differentiation process or with the electrophysiological features of the included cells. Such evidence confirmed that 3D bioprinting can be considered a good model for the study of ALS pathogenesis.
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- 2022
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27. Lysosomes Dysfunction Causes Mitophagy Impairment in PBMCs of Sporadic ALS Patients.
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Bordoni M, Pansarasa O, Scarian E, Cristofani R, Leone R, Fantini V, Garofalo M, Diamanti L, Bernuzzi S, Gagliardi S, Carelli S, Poletti A, and Cereda C
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- Animals, Humans, Leukocytes, Mononuclear metabolism, Lysosomes metabolism, TOR Serine-Threonine Kinases metabolism, Trehalose metabolism, Amyotrophic Lateral Sclerosis metabolism, Mitophagy
- Abstract
Mitochondria alterations are present in tissues derived from patients and animal models, but no data are available for peripheral blood mononuclear cells (PBMCs) of ALS patients. This work aims to investigate mitophagy in PBMCs of sporadic (sALS) patients and how this pathway can be tuned by using small molecules. We found the presence of morphologically atypical mitochondria by TEM and morphological abnormalities by MitoTracker™. We found a decreased number of healthy mitochondria in sALS PBMCs and an impairment of mitophagy with western blot and immunofluorescence. After rapamycin treatment, we found a higher increase in the LC3 marker in sALS PBMCs, while after NH4Cl treatment, we found a lower increase in the LC3 marker. Finally, mTOR-independent autophagy induction with trehalose resulted in a significant decrease in the lysosomes level sALS PBMCs. Our data suggest that the presence of morphologically altered mitochondria and an inefficient turnover of damaged mitochondria in PBMCs of sALS patients rely on the impairment of the mitophagy pathway. We also found that the induction of the mTOR-independent autophagy pathway leads to a decrease in lysosomes level, suggesting a more sensitivity of sALS PBMCs to trehalose. Such evidence suggests that trehalose could represent an effective treatment for ALS patients.
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- 2022
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28. Extracellular Vesicles Derived From Plasma of Patients With Neurodegenerative Disease Have Common Transcriptomic Profiling.
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Sproviero D, Gagliardi S, Zucca S, Arigoni M, Giannini M, Garofalo M, Fantini V, Pansarasa O, Avenali M, Ramusino MC, Diamanti L, Minafra B, Perini G, Zangaglia R, Costa A, Ceroni M, Calogero RA, and Cereda C
- Abstract
Objectives: There is a lack of effective biomarkers for neurodegenerative diseases (NDs) such as Alzheimer's disease (AD), Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS), and frontotemporal dementia. Extracellular vesicle (EV) RNA cargo can have an interesting potential as a non-invasive biomarker for NDs. However, the knowledge about the abundance of EV-mRNAs and their contribution to neurodegeneration is not clear., Methods: Large and small EVs (LEVs and SEVs) were isolated from plasma of patients and healthy volunteers (control, CTR) by differential centrifugation and filtration, and RNA was extracted. Whole transcriptome was carried out using next generation sequencing (NGS)., Results: Coding RNA (i.e., mRNA) but not long non-coding RNAs (lncRNAs) in SEVs and LEVs of patients with ALS could be distinguished from healthy CTRs and from other NDs using the principal component analysis (PCA). Some mRNAs were found in commonly deregulated between SEVs of patients with ALS and frontotemporal dementia (FTD), and they were classified in mRNA processing and splicing pathways. In LEVs, instead, one mRNA and one antisense RNA (i.e., MAP3K7CL and AP003068.3) were found to be in common among ALS, FTD, and PD. No deregulated mRNAs were found in EVs of patients with AD., Conclusion: Different RNA regulation occurs in LEVs and SEVs of NDs. mRNAs and lncRNAs are present in plasma-derived EVs of NDs, and there are common and specific transcripts that characterize LEVs and SEVs from the NDs considered in this study., Competing Interests: SZ was employed by EnGenome SRL. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Sproviero, Gagliardi, Zucca, Arigoni, Giannini, Garofalo, Fantini, Pansarasa, Avenali, Ramusino, Diamanti, Minafra, Perini, Zangaglia, Costa, Ceroni, Calogero and Cereda.)
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- 2022
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29. Professional responsibility between guidelines and customary practice. A conflict of interest?
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Tebala GD, Slack Z, Fantini V, Masato S, Parla M, Cirocchi R, and Di Saverio S
- Abstract
In Countries with Common Law, the principles of medical liability in case of malpractice claim are based on the Bolam/Bolitho tests, that is, the opinion of a panel of average professionals of the same specialty. On the contrary, in Countries whose legal system is based on the Corpus Iustinianeum the practice of a doctor is benchmarked against established guidelines. Occasionally, the opinion of an expert panel may not overlap the formal guidelines, in particular in cases like the surgical treatment of acute diverticulitis and that of acute cholecystitis where pre-existing old-fashioned ideas are so rooted into the behaviour of doctors that they are extremely difficult to eradicate despite the growing amount of evidence. This may lead to the paradox that a doctor who followed the guidelines might be considered imprudent or negligent as his or her choice did not overlap that of the "average" professional. This is a grey area that needs clarification. We propose that the "expert panel" nominated during a medical malpractice claim should not report their personal - although shared - opinion, but should unbiasedly report all the available acceptable options. Criminal and civil courts, along with other medical panels, must consider this bias when scrutinizing the practice of a professional., (Copyright © 2021 Elsevier Ltd. All rights reserved.)
- Published
- 2021
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30. Hospital discharge: testing the "Blaylock Risk Assessment Screening Score" in a surgical department.
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Colognesi S, Fagnani C, Panceri F, Ruggero M, Di Florio F, Passoni C, Fantini V, Boracchi P, Orenti A, Fumagalli MA, and Vergani M
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- Adolescent, Humans, Length of Stay, Reproducibility of Results, Risk Assessment, Hospitals, Patient Discharge
- Abstract
Background and aim of the work Standardizing patients' assessment to identify individuals at greater risk in encountering difficulties at discharge may help to assist healthcare professionals in clinical decision making and address the gaps in quality that negatively affect continuity of care. We analyzed the predictive validity and the test-retest reliability of the BRASS index in surgical inpatients. Moreover, we evaluated the association between other variables and length of stay or location at discharge. Methods A prospective observational study was conducted. Four hundred twenty-eight patients (≥18 years old) hospitalized in the surgical department of Vimercate hospital were recruited. Data were collected using BRASS index within 48 hours from admission and before discharge. Results We found a high specificity for BRASS in identifying patients discharged to their home with assistance or to residential care. The hospital stay for medium and high-risk patients was significantly longer than those in the low-risk group. There was no statistically significant difference of the BRASS scores during hospitalization. Type of admission, pressure ulcers, ASA score, multidrug-resistant bacterial infections, medical complications and Intensive Unit Care stay showed a significant correlation with longer hospitalization and increased probability to be discharged to their home with assistance or to residential care. Conclusions The BRASS Index may support healthcare professionals to identify surgical inpatients requiring a discharge planning and needs to be completed just once at admission. The inclusion of other patient-specific factors in the assessment process could be valuable for targeting the at-risk population.
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- 2021
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31. Abbiategrasso Brain Bank Protocol for Collecting, Processing and Characterizing Aging Brains.
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Poloni TE, Medici V, Carlos AF, Davin A, Ceretti A, Mangieri M, Cassini P, Vaccaro R, Zaccaria D, Abbondanza S, Bordoni M, Fantini V, Fogato E, Cereda C, Ceroni M, and Guaita A
- Subjects
- Aged, Brain pathology, Humans, Neurodegenerative Diseases pathology, Aging pathology, Brain cytology, Specimen Handling methods, Tissue Banks
- Abstract
In a constantly aging population, the prevalence of neurodegenerative disorders is expected to rise. Understanding disease mechanisms is the key to find preventive and curative measures. The most effective way to achieve this is through direct examination of diseased and healthy brain tissue. The authors present a protocol to obtain, process, characterize and store good quality brain tissue donated by individuals registered in an antemortem brain donation program. The donation program includes a face-to-face empathic approach to people, a collection of complementary clinical, biological, social and lifestyle information and serial multi-dimensional assessments over time to track individual trajectories of normal aging and cognitive decline. Since many neurological diseases are asymmetrical, our brain bank offers a unique protocol for slicing fresh specimens. Brain sections of both hemispheres are alternately frozen (at -80 °C) or fixed in formalin; a fixed slice on one hemisphere corresponds to a frozen one on the other hemisphere. With this approach, a complete histological characterization of all frozen material can be obtained, and omics studies can be performed on histologically well-defined tissues from both hemispheres thus offering a more complete assessment of neurodegenerative disease mechanisms. Correct and definite diagnosis of these diseases can only be achieved by combining the clinical syndrome with the neuropathological evaluation, which often adds important etiological clues necessary to interpret the pathogenesis. This method can be time consuming, expensive and limited as it only covers a limited geographical area. Regardless of its limitations, the high degree of characterization it provides can be rewarding. Our ultimate goal is to establish the first Italian Brain Bank, all the while emphasizing the importance of neuropathologically verified epidemiological studies.
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- 2020
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32. 3D Printed Conductive Nanocellulose Scaffolds for the Differentiation of Human Neuroblastoma Cells.
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Bordoni M, Karabulut E, Kuzmenko V, Fantini V, Pansarasa O, Cereda C, and Gatenholm P
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- Cell Differentiation drug effects, Cell Proliferation drug effects, Cell Survival drug effects, Cells, Cultured, Humans, Mesenchymal Stem Cells drug effects, Tissue Scaffolds, Cellulose pharmacology, Mesenchymal Stem Cells cytology, Nanotubes, Carbon adverse effects, Neuroblastoma pathology, Printing, Three-Dimensional
- Abstract
We prepared cellulose nanofibrils-based (CNF), alginate-based and single-walled carbon nanotubes (SWCNT)-based inks for freeform reversible embedding hydrogel (FRESH) 3D bioprinting of conductive scaffolds. The 3D printability of conductive inks was evaluated in terms of their rheological properties. The differentiation of human neuroblastoma cells (SH-SY5Y cell line) was visualized by the confocal microscopy and the scanning electron microscopy techniques. The expression of TUBB3 and Nestin genes was monitored by the RT-qPCR technique. We have demonstrated that the conductive guidelines promote the cell differentiation, regardless of using differentiation factors. It was also shown that the electrical conductivity of the 3D printed scaffolds could be tuned by calcium-induced crosslinking of alginate, and this plays a significant role on neural cell differentiation. Our work provides a protocol for the generation of a realistic in vitro 3D neural model and allows for a better understanding of the pathological mechanisms of neurodegenerative diseases.
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- 2020
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33. [The assessment of planned discharges: agreement between BRASS index and clinical judgement].
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Colognesi S, Fagnani C, Panceri F, Fantini V, Ruggero M, Di Florio F, Boracchi P, Orenti A, and Vergani M
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- Adolescent, Adult, Age Factors, Aged, Aged, 80 and over, Female, Humans, Italy, Male, Middle Aged, Social Support, Young Adult, Nursing Assessment methods, Patient Discharge statistics & numerical data, Risk Assessment methods
- Abstract
. The assessment of planned discharges: agreement between BRASS index and clinical judgement., Introduction: The BRASS (Blaylock Risk Assessment Screening Score) index is a risk screening tool for identification of patients at risk of difficult discharge., Objective: To assess the agreement of discharge options between the BRASS classification and the nurses' clinical judgement., Methods: Consecutive patients admitted to surgical wards of a large Italian hospital from November 2016 to June 2017 were assessed with the BRASS index within 48 hours from admission and 24 hours before discharge. After discharge a group of expert nurses, blind to the BRASS score and patient effective destination, planned the patients' discharge reviewing the clinical records. Patients were classified as needing ordinary discharge, with support or to hospital., Results: The 90% of the 428 included patients remained in the same BRASS risk class; the agreement between BRASS score (K Cohen) at admission and clinical judgement was 0.474 and 0.533 at discharge. Of the 96 subject at need of discharge with support or to hospital, 60 were classified in the same class by the BRASS Index. Factors that impacted on the different classification were patients' age, presence of devices and availability and appropriateness of social support., Conclusions: Most patients remained in the same BRASS class. There is moderate agreement between BRASS index and clinical judgement, specifically for patients at medium/high risk of difficult discharge. Our data confirm the need for including new items in the Brass scale.
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- 2019
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34. Bioink Composition and Printing Parameters for 3D Modeling Neural Tissue.
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Fantini V, Bordoni M, Scocozza F, Conti M, Scarian E, Carelli S, Di Giulio AM, Marconi S, Pansarasa O, Auricchio F, and Cereda C
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- Alginates chemistry, Cell Line, Tumor, Cells, Cultured, Gelatin chemistry, Humans, Induced Pluripotent Stem Cells cytology, Neural Stem Cells cytology, Primary Cell Culture methods, Printing, Three-Dimensional
- Abstract
Neurodegenerative diseases (NDs) are a broad class of pathologies characterized by the progressive loss of neurons in the central nervous system. The main problem in the study of NDs is the lack of an adequate realistic experimental model to study the pathogenic mechanisms. Induced pluripotent stem cells (iPSCs) partially overcome the problem, with their capability to differentiate into almost every cell types; even so, these cells alone are not sufficient to unveil the mechanisms underlying NDs. 3D bioprinting allows to control the distribution of cells such as neurons, leading to the creation of a realistic in vitro model. In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells. All cells were encapsulated inside the bioink, printed and cultivated for at least seven days; they all presented good viability. We also evaluated the maintenance of the printed shape, opening the possibility to obtain a reliable in vitro neural tissue combining 3D bioprinting and iPSCs technology, optimizing the study of the degenerative processes that are still widely unknown.
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- 2019
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35. From Neuronal Differentiation of iPSCs to 3D Neuro-Organoids: Modelling and Therapy of Neurodegenerative Diseases.
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Bordoni M, Rey F, Fantini V, Pansarasa O, Di Giulio AM, Carelli S, and Cereda C
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- Animals, Humans, Cell Differentiation, Induced Pluripotent Stem Cells cytology, Models, Neurological, Neurodegenerative Diseases pathology, Neurodegenerative Diseases therapy, Neurons cytology, Organoids cytology
- Abstract
In the last decade, the advances made into the reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) led to great improvements towards their use as models of diseases. In particular, in the field of neurodegenerative diseases, iPSCs technology allowed to culture in vitro all types of patient-specific neural cells, facilitating not only the investigation of diseases' etiopathology, but also the testing of new drugs and cell therapies, leading to the innovative concept of personalized medicine. Moreover, iPSCs can be differentiated and organized into 3D organoids, providing a tool which mimics the complexity of the brain's architecture. Furthermore, recent developments in 3D bioprinting allowed the study of physiological cell-to-cell interactions, given by a combination of several biomaterials, scaffolds, and cells. This technology combines bio-plotter and biomaterials in which several types of cells, such as iPSCs or differentiated neurons, can be encapsulated in order to develop an innovative cellular model. IPSCs and 3D cell cultures technologies represent the first step towards the obtainment of a more reliable model, such as organoids, to facilitate neurodegenerative diseases' investigation. The combination of iPSCs, 3D organoids and bioprinting will also allow the development of new therapeutic approaches. Indeed, on the one hand they will lead to the development of safer and patient-specific drugs testing but, also, they could be developed as cell-therapy for curing neurodegenerative diseases with a regenerative medicine approach.
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- 2018
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36. Is Treatment With Dithiothreitol More Effective Than Sonication for the Diagnosis of Prosthetic Joint Infection?
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Sambri A, Cadossi M, Giannini S, Pignatti G, Marcacci M, Neri MP, Maso A, Storni E, Gamberini S, Naldi S, Torri A, Zannoli S, Tassinari M, Fantini M, Bianchi G, Donati D, and Sambri V
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- Adolescent, Adult, Aged, Aged, 80 and over, Arthroplasty, Replacement, Hip instrumentation, Arthroplasty, Replacement, Knee instrumentation, Female, Humans, Italy, Male, Middle Aged, Predictive Value of Tests, Prospective Studies, Prosthesis-Related Infections microbiology, Prosthesis-Related Infections surgery, Reoperation, Reproducibility of Results, Young Adult, Arthroplasty, Replacement, Hip adverse effects, Arthroplasty, Replacement, Knee adverse effects, Bacteria isolation & purification, Bacteriological Techniques, Dithiothreitol administration & dosage, Hip Prosthesis adverse effects, Knee Prosthesis adverse effects, Prosthesis-Related Infections diagnosis, Sonication
- Abstract
Background: Prosthetic joint infection (PJI) is among the most-severe complications of a total joint arthroplasty. Identification of the causal organism is of paramount importance for successful treatment, and sonication of implants may aid in this identification. Dithiothreitol (DTT) treatment has been proposed as an alternative to sonication to improve diagnosis, reduce costs, and improve reliability of the procedure, but its efficacy remains poorly characterized., Questions/purposes: (1) Are DTT and sonication more sensitive and/or more specific than standard cultures of tissue samples for the diagnosis of PJI? (2) Which test (DTT or sonication) is more sensitive when the clinician does not suspect infection before surgery? (3) Which test (DTT or sonication) is more sensitive when the clinician suspects infection before surgery?, Methods: Two hundred thirty-two patients undergoing revision of a knee or hip arthroplasty were prospectively evaluated in this randomized study. Cultures were performed on five tissue samples from each patient and on fluid obtained by prosthesis treatment in patients randomly assigned to sonication (117 patients) or DTT (115 patients). The reference standard against which cultures (on tissue samples and on fluids from sonication or DTT) were compared was the Musculoskeletal Infection Society definition of PJI., Results: Cultures on sonication and DTT fluids provided higher sensitivity (89% and 91%, respectively) than those on standard cultures of tissue samples (79%; p < 0.001). Among patients in whom infection was not suspected before surgery, the sensitivity of DTT was greater than that for sonication and cultures on tissue samples (100% versus 70% and 50%; p < 0.001). Among patients in whom infection was suspected before surgery, the sensitivity of DTT and sonication were not greater than that for standard cultures (89% and 94% versus 86%)., Conclusions: In this randomized study, we found no difference in sensitivity between DTT and sonication for the detection of PJI, and both of those tests were more sensitive than standard tissue cultures. Thus, cultures of sonication or DTT fluid should be considered important additional tools to standard cultures for definition of PJI and should be considered together with other criteria, especially in settings where infection is not suspected before revision surgery.Level of Evidence Level I, diagnostic study.
- Published
- 2018
- Full Text
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