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To investigate the spatial and temporal changes in fractional vegetation coverage (FVC) and their driving forces in different regions of the Inner Mongolia section of the Yellow River Basin, this paper observed the spatial trends and stability of FVC in these regions based on the MOD13Q1 information regarding the 2000–2020 period as a data source. It used the dimidiate pixel model to invert FVC, and based on the centre of gravity migration model, the coefficient of variation and the Mann–Kendall and Sen's slope estimator test, it studied the spatial variation trend and stability of FVC in the four relevant areas of the Inner Mongolia section; an attribution analysis using a geodetector was also conducted. The following results were found: (1) in terms of temporal FVC change in the relevant areas, from 2000 to 2020, the overall FVC showed an increasing trend, indicating an obvious hierarchy of change as per different seasonal scales (summer > growing season > fall > spring). There is a mutation point in FVC in different areas, and the FVC sequence is random. (2) Regarding spatial change, the overall FVC showed a trend of being high in the eastern regions and low in the western regions and low–high–low from the north to the south; the stability of the Hetao Irrigation District–Wuliangsuhai Area changed more significantly with the successive seasons, and the degraded areas of FVC were mainly distributed in the city centre of the Kundulun River–Daheihe River Area and in the Hetao Irrigation District in the summer. (3) In terms of driving factors, soil type had a relatively higher explanatory power regarding the Hetao Irrigation District–Wuliangsuhai Area, rainfall had a relatively higher explanatory power regarding the Morin River–Wuding River Area and the Kundulun River–Daheihe River Area, and land use had a relatively higher explanatory power regarding the Ten Kongtui–Heidaigou Area. [ABSTRACT FROM AUTHOR]

Objective: Based on the antibacterial analysis, we screened Polyketide synthase (PKS I) gene from the epiphytic bacteria of Porphyra spp., in order to obtain the PKS I positive strains and detect the potential connection between the PKS pathway and the antibacterial mechanisms., Methods: A total of 31 bacteria with broad-spectrum antibacterial activity were screened by agar-screening methods. The 16S rDNA and the Ketosynthase gene were amplified from the genome DNA of these bacteria, which were cloned into pMD19-T vector for sequencing analysis., Results: Porphyra spp. epiphytic bacteria showed broad-spectrum antibacterial activity. Three PKS I positive epiphytic bacteria were obtained from Wenzhou rotten Porphyra spp. samples which had high antibacterial activity. The BLAST results indicated that the Ketosynthase fragments of PKS I from the strains of WPhG3, WPySwl and WPySw2 shared highest similarity (98%, 99%, 98%) to the strains of Bacillus subtilis subsp. Subtilis str. 168 (NP_389602), Bacillus subtilis (ABR19776) and Aspergillus carbonarius (AAZ99721), respectively. Furthermore, the phylogenetic analysis based on 16S rDNA sequences indicated that they belonged to the genus of Bacillus., Conclusion: The flora of Porphyra spp. epiphytic bacteria was complex, which regulated the phycosphere in many ways. The PKS I pathway might be a performance of antibacterial function of Bacillus from Wenzhou rotten samples.