1,233 results on '"Fang, Du"'
Search Results
2. Uncovering essential anesthetics-induced exosomal miRNAs related to hepatocellular carcinoma progression: a bioinformatic investigation
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Ning Huang, Jie Fang, Fang Du, Jichuan Zhou, Yuxin Li, and Xiaoguang Zhang
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Hepatocellular carcinoma ,Anesthetic ,Sevoflurane ,Exosome ,miRNA ,miR-21-4-5p ,Internal medicine ,RC31-1245 ,Genetics ,QH426-470 - Abstract
Abstract Background Anesthetic drugs may alter exosomal microRNA (miRNA) contents and mediate cancer progression and tumor microenvironment remodeling. Our study aims to explore how the anesthetics (sevoflurane and propofol) impact the miRNA makeup within exosomes in hepatocellular carcinoma (HCC), alongside the interconnected signaling pathways linked to the tumor immune microenvironment. Methods In this prospective study, we collected plasma exosomes from two groups of HCC patients (n = 5 each) treated with either propofol or sevoflurane, both before anesthesia and after hepatectomy. Exosomal miRNA profiles were assessed using next-generation sequencing (NGS). Furthermore, the expression data from The Cancer Genome Atlas-Liver Hepatocellular Carcinoma (TCGA-LIHC) was used to pinpoint the differentially expressed exosomal miRNAs (DEmiRNAs) attributed to the influence of propofol or sevoflurane in the context of HCC. Gene set enrichment analysis (GSEA) and gene set variation analysis (GSVA) were used to dissect the signaling pathways and biological activities associated with the identified DEmiRNAs and their corresponding target genes. Results A total of 35 distinct DEmiRNAs were exclusively regulated by either propofol (n = 9) or sevoflurane (n = 26). Through TCGA-LIHC database analysis, 8 DEmiRNAs were associated with HCC. These included propofol-triggered miR-452-5p and let-7c-5p, as well as sevoflurane-induced miR-24-1-5p, miR-122-5p, miR-200a-3p, miR-4686, miR-214-3p, and miR-511-5p. Analyses revealed that among these 8 DEmiRNAs, the upregulation of miR-24-1-5p consistently demonstrated a significant association with lower histological grades (p
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- 2024
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3. Transparent Reporting of the Early-stage Clinical Evaluation of Clinical Decision Support Systems Based on Artificial Intelligence
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LEI Fang, DU Liang, DONG Min, LIU Xuemei
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artificial intelligence ,decision support systems, clinical ,early-stage clinical evaluation ,decide-ai ,transparency ,Medicine - Abstract
With the wide application of artificial intelligence (AI) in the medical field, more and more AI-based clinical decision support systems have been applied in the clinical diagnosis, screening, and other fields. Early-stage clinical evaluation is important for evaluating the clinical performance, safety, and human factors of AI-based clinical decision support systems, and laying the foundation for large-scale trials. However, the transparency and integrity of the clinical reports need to be improved. The Developmental and Exploratory Clinical Investigations of DEcision Support Systems Driven by Artificial Intelligence (DECIDE-AI) was officially published online in May 2022. Based on this guideline and relative literature, this paper explores the transparent reporting of early-stage clinical evaluation of AI-based clinical decision support systems, in order to help developers and researchers better understand and apply the relevant guidelines, and improve the reporting transparency of early-stage clinical evaluation of AI-based clinical decision support systems.
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- 2024
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4. Two decades of progress in glioma methylation research: the rise of temozolomide resistance and immunotherapy insights
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Xianhao Huo, Haoyuan Li, Yixiang Xing, Wenqing Liu, Pengfei Chen, Fang Du, Lijuan Song, Zhenhua Yu, Xiangmei Cao, and Jihui Tian
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glioma methylation ,MGMT promoter methylation ,temozolomide ,immunotherapy ,bibliometric analysis ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
AimsThis study aims to systematically analyze the global trends in glioma methylation research using bibliometric methodologies. We focus on identifying the scholarly trajectory and key research interests, and we utilize these insights to predict future research directions within the epigenetic context of glioma.MethodsWe performed a comprehensive literature search of the Web of Science Core Collection (WoSCC) to identify articles related to glioma methylation published from January 1, 2004, to December 31, 2023. The analysis included full-text publications in the English language and excluded non-research publications. Analysis and visualization were performed using GraphPad Prism, CiteSpace, and VOSviewer software.ResultsThe search identified 3,744 publications within the WoSCC database, including 3,124 original research articles and 620 review articles. The research output gradually increased from 2004 to 2007, followed by a significant increase after 2008, which peaked in 2022. A minor decline in publication output was noted during 2020–2021, potentially linked to the coronavirus disease 2019 pandemic. The United States and China were the leading contributors, collectively accounting for 57.85% of the total research output. The Helmholtz Association of Germany, the German Cancer Research Center (DKFZ), and the Ruprecht Karls University of Heidelberg were the most productive institutions. The Journal of Neuro-Oncology led in terms of publication volume, while Neuro-Oncology had the highest Impact Factor. The analysis of publishing authors revealed Michael Weller as the most prolific contributor. The co-citation network analysis identified David N. Louis's article as the most frequently cited. The keyword analysis revealed “temozolomide,” “expression,” “survival,” and “DNA methylation” as the most prominent keywords, while “heterogeneity,” “overall survival,” and “tumor microenvironment” showed the strongest citation bursts.ConclusionsThe findings of this study illustrate the increasing scholarly interest in glioma methylation, with a notable increase in research output over the past two decades. This study provides a comprehensive overview of the research landscape, highlighting the importance of temozolomide, DNA methylation, and the tumor microenvironment in glioma research. Despite its limitations, this study offers valuable insights into the current research trends and potential future directions, particularly in the realm of immunotherapy and epigenetic editing techniques.
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- 2024
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5. Anthocyanin Accumulation and Chlorophyll Degradation Lead to the Formation of Colourful Leaves of Syringa oblata in Autumn
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Meiling Han, Rui Lu, Meng Han, Xiuyun Yang, Fang Du, Xiaoping Chen, Saiwei Huang, Shan Luo, and Dongliang Han
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Syringa oblata ,colour-leaf plant ,leaf colouration ,anthocyanin ,chlorophyll ,polyphenol. ,Botany ,QK1-989 - Abstract
Abstract Syringa oblata is an important garden plant whose leaf colour turns from green to red in autumn when air temperature and daylength decrease. This study explored the reasons for leaf reddening by detecting phenotypic characteristics and pigment types and contents. With leaf reddening, luminance L* increased and chrominance a* decreased significantly. Chlorophyll and carotenoid contents significantly decreased in accordance with the distribution change of green pigment in leaf cells. Conversely, the red pigment distribution increased and the total polyphenol, total flavonoid and total anthocyanin contents evidently increased. Anthocyanin accumulation was the important reason for leaf reddening. Of the anthocyanins detected in leaves, cyanidin and delphinidin-3-O-rutinoside contents gradually increased with leaf reddening and were negatively correlated with L*. They were considered key anthocyanins influencing leaf colour. Apigenin and syringic acid were correlated with delphinidin-3-O-rutinoside and cyanidin, and they could be the anthocyanin co-pigments. Cyanidin-3-O-arabinoside and taxifolin were more abundant polyphenols in leaves. In summary, anthocyanin accumulation and chlorophyll degradation occurred along with leaf reddening. Temperature, light, and other co-pigments influenced the anthocyanin and chlorophyll contents. This study provides evidence for applications of S. oblata as a coloured-leaf plant in gardens and as a source of active ingredients in the commercial market.
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- 2024
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6. Convolutional neural network incorporating misclassification information for image recognition.
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Junying Hu, Rongrong Fei, Fang Du, Peiju Chang, and Jiangshe Zhang 0001
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- 2024
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7. Back-propagating errors through artificial neural networks for variable selection.
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Junying Hu, Peiju Chang, Fang Du, Rongrong Fei, Kai Sun 0007, Jiangshe Zhang 0001, and Hai Zhang 0001
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- 2024
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8. Characteristic Analysis of the MS6.8 Luding Earthquake Sequence in Sichuan, China, on Sept. 5, 2022
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Min Zhao, Feng Long, Yue Gong, and Fang Du
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the ms6.8 luding earthquake ,sequence analysis ,the focal mechanisms ,seismogenic tectonics ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Risk in industry. Risk management ,HD61 - Abstract
The MS6.8 earthquake occurred in Luding County, Ganzi Prefecture, Sichuan Province, China at 12:52 p.m. on Sept. 5, 2022, is preliminarily analyzed in terms of regional tectonics, historical earthquakes, sequence characteristics and focal mechanism solutions. The results show that: 1) The magnitude difference ΔM between the main shock and the maximum aftershock, the energy ratio ER of the main shock to the aftershocks, the p-value and the estimated Mmax-value indicate that the MS6.8 Luding earthquake sequence can be divided into the main shock-aftershock type (MAT). 2) The spatial distribution of the aftershocks, their corresponding b-value and expected maximum magnitude are obviously segmented, which reflect the complexity of the seismogenic tectonics. 3) According to the focal mechanisms obtained from the HASH program, the geometry distribution of the sequence, and the relationship between the sequence and the nearby faults, it can be inferred that the Moxi fault segment of the Xianshuihe fault zone is the seismogenic tectonics of the MS6.8 Luding earthquake sequence.
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- 2024
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9. Characteristic Analysis of the MS6.0 Ma'erkang Earthquake Sequence on Jun. 10, 2022
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Yu-ping Qi, Feng Long, Jun Li, Jun Su, Di Wang, Ling-zhe Kong, Meng-die Chen, and Fang Du
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the ms6.0 ma'erkang earthquake ,earthquake sequence ,characteristic analysis ,source parameters ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Risk in industry. Risk management ,HD61 - Abstract
On June 10, 2022, the MS6.0 Ma'erkang earthquake sequence occurred in the Bayan Har block. In this paper, the temporal and spatial distribution and attenuation characteristics of earthquake sequence is analyzed based on the regional structure, the temporal and spatial distribution of earthquake sequence, the focal mechanism solution, and the parameters of earthquake sequence, using the data of Sichuan Seismic Network and the temporary stations incorporated into the network. The results show that: (1) The MS6.0 Ma'erkang earthquake sequence is generally distributed in NW-SE direction, and the long axis of this series is basically consistent with the nearby Songgang fault. (2) As the earthquake sequence is formed by three large events with MS ≥5.0, the frequency of small earthquakes in the earthquake sequence area generally attenuates relatively slowly, while the activity level (magnitude) of aftershocks attenuates rapidly. (3) After the MS5.2 earthquake, the sequence parameters obtained show that the h-value is 1.09 and the p-value is stable at 1.02, indicating that the intensity and frequency attenuation of the earthquake sequence are gradually stable and tend to be normal. The b-value is 0.95, indicating that the maximum aftershock magnitude of the series is estimated to be ML5.1, and the b-value gradually tended to be stable, indicating that the stress in the region gradually tended to be balanced after the MS5.2 earthquake. The MS4.4 (ML5.0) earthquake that occurred at 4:37 on June 10 (local time) is the largest aftershock after the MS5.2 earthquake in the sequence. (4) The Songgang fault with NW-SE trend is presumed to be the main seismogenic tectonics, but the migration of three earthquakes with MS≥5.0 may also indicate that the Songgang fault is not a single seismogenic tectonics, which requires further field scientific investigation and analysis.
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- 2024
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10. Analysis of related factors for neuropsychiatric comorbidities in children with epilepsy
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Xin-Ying Zhang, Meng Sun, Jiang-Ya Wang, Fang-Fang Du, Xue-Fei Liu, Ling-Jun Wang, Zhen-De Hou, and Ya-Ying Cheng
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Children ,Cognitive impairment ,Epilepsy ,Psycho behavioral disorder ,Risk factors ,Medicine - Abstract
Abstract Objective To analyze the risk factors affecting psychiatric behavior and study the psychobehavioral conditions of children with epilepsy. Method We randomly selected and enrolled 294 children with epilepsy who visited and were hospitalized in the pediatric clinic of Hebei General Hospital between January 2017 and January 2022, as the study participants. We comprehensively assessed their cognitive functions using the Gesell development schedule or Wechsler Intelligence Scales. The participants were divided into the study group (n = 123) with cognitive impairment and the control group (n = 171) with normal cognitive functions, for analysis. Results There were statistically significant differences between the two groups in disease course, frequency of epilepsy, status epilepticus, and the number of antiseizure medications (ASMs) used (P 0.05). Based on multivariate logistic regression analysis, the course of disease, frequency of onset, status epilepticus and number of ASMs used were identified as high-risk factors for cognitive impairment in children with epilepsy. Similarly, early onset, long course of disease, known etiology, and combination of multiple drugs have a negative impact on behavioral problems, school education, and social adaptability. Conclusion The course of disease, the frequency of onset, status epilepticus, and the number of ASMs used are high-risk factors for cognitive impairment in children with epilepsy, which can be prevented and controlled early. When selecting ASMs, their advantages and disadvantages should be weighed. Moreover, the availability of alternative treatment options must be considered. With the help of genomic technology, the causes of epilepsy should be identified as early as possible, and precision medicine and gene therapy for children with epilepsy should be actively developed.
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- 2024
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11. Glucocorticoid stress hormones stimulate vesicle-free Tau secretion and spreading in the brain
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Qing Yu, Fang Du, Irla Belli, Patricia A. Gomes, Ioannis Sotiropoulos, and Clarissa L. Waites
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Cytology ,QH573-671 - Abstract
Abstract Chronic stress and elevated levels of glucocorticoids (GCs), the main stress hormones, accelerate Alzheimer’s disease (AD) onset and progression. A major driver of AD progression is the spreading of pathogenic Tau protein between brain regions, precipitated by neuronal Tau secretion. While stress and high GC levels are known to induce intraneuronal Tau pathology (i.e. hyperphosphorylation, oligomerization) in animal models, their role in trans-neuronal Tau spreading is unexplored. Here, we find that GCs promote secretion of full-length, primarily vesicle-free, phosphorylated Tau from murine hippocampal neurons and ex vivo brain slices. This process requires neuronal activity and the kinase GSK3β. GCs also dramatically enhance trans-neuronal Tau spreading in vivo, and this effect is blocked by an inhibitor of Tau oligomerization and type 1 unconventional protein secretion. These findings uncover a potential mechanism by which stress/GCs stimulate Tau propagation in AD.
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- 2024
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12. High expression of centromere protein A and its molecular mechanism and clinical significance in prostate cancer: A study based on data mining and immunohistochemistry
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Fang‐Cheng Jiang, Gao‐Qiang Zhai, Jia‐Lin Liu, Rui‐Gong Wang, Yuan‐Ping Yang, Harivignesh Murugesan, Xiao‐Xiang Yu, Xiu‐Fang Du, Juan He, Zhen‐Bo Feng, Shang Ling Pan, Gang Chen, Sheng‐Hua Li, and Zhi‐Guang Huang
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centromere protein A (CENPA) ,data mining ,immunohistochemistry ,prostate cancer (PCa) ,single‐cell RNA sequencing ,Biology (General) ,QH301-705.5 - Abstract
Abstract The progression of prostate cancer (PCa) leads to poor prognosis. However, the molecular mechanism of PCa is still not completely clear. This study aimed to elucidate the important role of centromere protein A (CENPA) in PCa. Large numbers of bulk RNA sequencing (RNA‐seq) data and in‐house immunohistochemistry data were used in analysing the expression level of CENPA in PCa and metastatic PCa (MPCa). Single‐cell RNA‐seq data was used to explore the expression status of CENPA in different prostate subpopulations. Enrichment analysis was employed to detect the function of CENPA in PCa. Clinicopathological parameters analysis was utilised in analysing the clinical value of CENPA. The results showed that CENPA was upregulated in PCa (standardised mean difference [SMD] = 0.83, p = 0.001) and MPCa (SMD = 0.61, p = 0.029). CENPA was overexpressed in prostate cancer stem cells (CSCs) with androgen receptor (AR) negative compared to epithelial cells with AR positive. CENPA may influence the development of PCa through affecting cell cycle. Patients with nodal metastasis had higher expression level of CENPA. And patients with high CENPA expression had poor disease‐free survival. Taken together, Overexpression of CENPA may influence the development of PCa by regulating cell cycle and promoting metastasis.
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- 2023
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13. A latent profile analysis of doctors’ joy in work at public hospitals
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Weilin Zhu, Jiayi Li, Liqun Wu, Fang Du, Yi Zhou, Kaichuan Diao, and Huatang Zeng
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public hospitals ,doctors ,joy in work ,latent profile analysis ,work stress ,Psychology ,BF1-990 - Abstract
IntroductionWhen doctors’ work stress increases, their joy in work decreases, severely affecting the quality of care and threatening patient safety. Analysis of the latent categories of joy in work of doctors in public hospitals and the differences in the characteristics of each category can help uncover hidden messages that enhance doctors’ joy in work.MethodsQuestionnaires were administered to 426 doctors working in public hospitals using the general information questionnaire and the public hospital doctor’s joy in work evaluation scale. Upon identifying their potential categories using latent profile analysis, chi-square test, and multinomial logistic regression were performed to analyze the differences in the characteristics of each category.ResultsThe 426 public hospital doctors could be divided into three potential categories: “low joy in work” (11.27%), “medium joy in work” (59.86%), and “high joy in work” (28.87%). Most of the doctors did not have much joy in work, with 71.13% of them having “low to medium joy in work.” Doctors who work in secondary or tertiary hospitals, have a personnel agency or contract, and are older than 45 years are more likely to belong to the “low joy in work” category. Some of the protective factors are having an average monthly income (RMB) of 10,001–15,000 yuan and having a fair or good self-rated health status.ConclusionThere are obvious classification characteristics of doctors’ level of joy in work. Hospital managers can take commensurate actions to improve their joy in work, thereby improving patient safety and the quality of medical services.
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- 2024
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14. Spatial transcriptomics reveals heterogeneity of histological subtypes between lepidic and acinar lung adenocarcinoma
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Linshan Xie, Hui Kong, Jinjie Yu, Mengting Sun, Shaohua Lu, Yong Zhang, Jie Hu, Fang Du, Qiuyu Lian, Hongyi Xin, Jian Zhou, Xiangdong Wang, Charles A. Powell, Fred R. Hirsch, Chunxue Bai, Yuanlin Song, Jun Yin, and Dawei Yang
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digital spatial profiler ,histological subtypes ,lung adenocarcinoma ,single‐cell RNA sequencing ,tumour endothelial cells ,Medicine (General) ,R5-920 - Abstract
Abstract Background Patients who possess various histological subtypes of early‐stage lung adenocarcinoma (LUAD) have considerably diverse prognoses. The simultaneous existence of several histological subtypes reduces the clinical accuracy of the diagnosis and prognosis of early‐stage LUAD due to intratumour intricacy. Methods We included 11 postoperative LUAD patients pathologically confirmed to be stage IA. Single‐cell RNA sequencing (scRNA‐seq) was carried out on matched tumour and normal tissue. Three formalin‐fixed and paraffin‐embedded cases were randomly selected for 10× Genomics Visium analysis, one of which was analysed by digital spatial profiler (DSP). Results Using DSP and 10× Genomics Visium analysis, signature gene profiles for lepidic and acinar histological subtypes were acquired. The percentage of histological subtypes predicted for the patients from samples of 11 LUAD fresh tissues by scRNA‐seq showed a degree of concordance with the clinicopathologic findings assessed by visual examination. DSP proteomics and 10× Genomics Visium transcriptomics analyses revealed that a negative correlation (Spearman correlation analysis: r = –.886; p = .033) between the expression levels of CD8 and the expression trend of programmed cell death 1(PD‐L1) on tumour endothelial cells. The percentage of CD8+ T cells in the acinar region was lower than in the lepidic region. Conclusions These findings illustrate that assessing patient histological subtypes at the single‐cell level is feasible. Additionally, tumour endothelial cells that express PD‐L1 in stage IA LUAD suppress immune‐responsive CD8+ T cells.
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- 2024
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15. sscNOVA: a semi-supervised convolutional neural network for predicting functional regulatory variants in autoimmune diseases
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Haibo Li, Zhenhua Yu, Fang Du, Lijuan Song, Yang Gao, and Fangyuan Shi
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autoimmune disease ,regulatory variant ,semi-supervised ,deep learning ,genome wide association studies ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Genome-wide association studies (GWAS) have identified thousands of variants in the human genome with autoimmune diseases. However, identifying functional regulatory variants associated with autoimmune diseases remains challenging, largely because of insufficient experimental validation data. We adopt the concept of semi-supervised learning by combining labeled and unlabeled data to develop a deep learning-based algorithm framework, sscNOVA, to predict functional regulatory variants in autoimmune diseases and analyze the functional characteristics of these regulatory variants. Compared to traditional supervised learning methods, our approach leverages more variants’ data to explore the relationship between functional regulatory variants and autoimmune diseases. Based on the experimentally curated testing dataset and evaluation metrics, we find that sscNOVA outperforms other state-of-the-art methods. Furthermore, we illustrate that sscNOVA can help to improve the prioritization of functional regulatory variants from lead single-nucleotide polymorphisms and the proxy variants in autoimmune GWAS data.
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- 2024
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16. Efficacy and safety of patient-controlled epidural analgesia versus patient-controlled intravenous analgesia following open hepatectomy: A single-center retrospective study
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Xue-Peng Zhang, Wan-Ting Wei, Yong Huang, Chang-Hong Miao, Xiao-Guang Zhang, and Fang Du
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Postoperative analgesia ,ERAS ,Opioid ,Patient-controlled analgesia ,Epidural ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Background: Postoperative analgesia is an essential component of enhanced recovery after surgery following abdominal surgery. Studies comparing the effectiveness of epidural analgesia with that of other analgesic modalities after liver surgery have reported inconsistent results. Consequently, the use of epidural analgesia for open hepatectomy is controversial. Objective: The present single-center retrospective study aimed to compare the efficacy and safety of patient-controlled epidural analgesia (PCEA) and patient-controlled intravenous analgesia (PCIA) in adults undergoing open hepatectomy. Methods: Patients who underwent open hepatectomy between January 2018 to December 2019 at Zhongshan Hospital, Fudan University were retrospectively analyzed. Propensity score matching was used to adjust baseline information between the PCEA and PCIA groups. The primary outcome measure was scores of the numeric rating scales (NRSs) for resting, exercise, and nocturnal pain at postoperative 24 h (postoperative day 1 [POD1]) and 48 h (POD2). The secondary outcome indicators included postoperative nausea and vomiting (PONV), hypotension, pruritus, respiratory depression, functional activity score (FAS), effective analgesic pump compression ratio, analgesic relief rate, discontinuation of the analgesic pump, reasons for discontinuation of the analgesic pump, and patient satisfaction with postoperative analgesia. Results: The NRS scores of the PCEA group on POD1 were significantly lower than those of the PCIA group (P
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- 2024
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17. Facile synthesis of fluorinated graphene for surface self-assembly of aluminum hydride
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Jian Gu, Xiang Guo, Wentao Xia, Panpan Peng, Fang Du, and Lei Li
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Fluorinated graphene ,Hydrothermal reaction ,Raman spectrum ,Aluminum hydride ,Explosives and pyrotechnics ,TP267.5-301 - Abstract
A facile method, refluxing with hydrothermal process, was used to synthesize the fluorinated graphene sheets (FGS) with high F/C ratio. Then, the surface of aluminum hydride (AlH3) was coated with FGS by means of liquid self-assembly process. The structure and performance of FGS were characterized through fourier transform infrared spectra (FTIR), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy, scanning electron microscopy (SEM), transmission electron microscope (TEM) and elemental analyzer. FTIR spectra show that the FGS have obvious absorption peak of CF bond. Raman spectra display many defects on FGS. XPS spectra and elemental analysis also prove the existence of CF bond, and the content of fluorine is more than 25%. From SEM photos we can see that FGS have thin layers with some curled wrinkles. TEM photos demonstrate intuitively the FGS has a few layers. At last, the aluminum hydride (AlH3) was coated with FGS by means of liquid self-assembly process, and AlH3 samples before and after treated were characterized through hydrogen content (H%) analysis, X-ray diffraction (XRD), FTIR, SEM and differential thermal analysis (DTA), ect. The results demonstrate that there is only a little FGS on the surface of AlH3, which won't affect the effective H% and release efficiency of hydrogen. AlH3@FGS also reduces the mechanical sensitivity of solid propellant with AlH3, which will remarkably promote the application of AlH3 in the novel high-energy solid propellant.
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- 2023
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18. Kerr-effect-based quantum logical gates in decoherence-free subspace
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Fang-Fang Du, Gang Fan, and Xue-Mei Ren
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Physics ,QC1-999 - Abstract
The decoherence effect caused by the coupling between the system and the environment undoubtedly leads to the errors in efficient implementations of two (or three) qubit logical gates in quantum information processing. Fortunately, decoherence-free subspace (DFS) introduced can effectively decrease the influence of decoherence effect. In this paper, we propose some schemes for setting up a family of quantum control gates, including controlled-NOT (CNOT), Toffoli, and Fredkin gates for two or three logical qubits by means of cross-Kerr nonlinearities in DFS. These three logical gates require neither complicated quantum computational circuits nor auxiliary photons (or entangled states). The success probabilities of three logical gates are approximate 1 by performing the corresponding classical feed-forward operations based on the different measuring results of the X-homodyne detectors, and their fidelities are robust against the photon loss with the current technology. The proposed logical gates rely on only simple linear-optics elements, available single-qubit operations, and mature measurement methods, making our proposed gates be feasible and efficient in practical applications.
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- 2024
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19. Immunosuppressive therapy selectively modulates B‐cell responses in patients with rheumatic and musculoskeletal diseases receiving the inactivated COVID‐19 vaccine
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Rui Li, Ruru Guo, Jia Li, Bing Zheng, Ronghao Xu, Xiaoyue Jiang, Yingying Chen, Guo Tang, Qianqian Li, Yixuan Li, Jiaqi Feng, Xiaoxiang Chen, Ying Wang, Shuang Ye, Fang Du, Liangjing Lu, and Jun Deng
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COVID‐19 vaccination ,humoral and cellular responses ,immunosuppressive medication ,rheumatic and musculoskeletal diseases ,Immunologic diseases. Allergy ,RC581-607 ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Abstract Background Immunosuppressive medication reduces the immunogenicity of the coronavirus disease 2019 (COVID‐19) vaccines in patients with rheumatic and musculoskeletal diseases (RMDs). However, the underlying mechanism remains unclear. The primary aim of our study was to dissect the impact of immunosuppressive medication on cellular and humoral immune responses in RMD patients receiving the inactivated COVID‐19 vaccine. Methods A total of 28 RMD patients and five healthy controls (HCs) receiving two doses of the inactivated COVID‐19 vaccine (Sinovac‐CoronaVac) were prospectively enrolled. Blood samples were collected before the primary vaccination (Week 0) and one week after the second vaccination (Week 5). Neutralizing antibody (nAb) titers and autoantibody titers were measured by a pseudovirus‐based neutralization assay and enzyme‐linked immunosorbent assay, respectively. CD4+ T‐cell and CD19+ B‐cell subsets and serum cytokines were analyzed by flow cytometry. Results The inactivated COVID‐19 vaccine was immunogenic in RMD patients and HCs after the second vaccination, but the nAb titers were lower in RMD patients than those in HCs. Only patients with systemic lupus erythematosus (SLE) had notably increased nAb titers. Remarkably, IgG+CD27+, IgG+IgG1+, and IgG+IgG1− B cells were reduced, whereas IgG−IgG1+ B cells, and total IgA and IgG titers were markedly increased. However, Tfh cell and Tfr cell subsets and cytokines produced by Tfh cells were not increased. The flare rate was low in RMD patients with comparable autoantibody titers, unchanged CD4+ T cell subsets and serum pro‐inflammatory cytokines (interleukin [IL]‐6, IL‐17, interferon‐γ, and tumor necrosis factor‐α) after the second vaccination. Conclusions Immunosuppressive therapy decreased the immunogenicity of the vaccine and maintained a low flare rate by selectively modulating B cell but not CD4+ T cell responses in RMD patients receiving the inactivated COVID‐19 vaccine. Optimization of the treatment regimen might ensure a durable and robust COVID‐19 vaccination response.
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- 2023
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20. Identification and Characterization of CD8+CD27+CXCR3− T Cell Dysregulation and Progression‐Associated Biomarkers in Systemic Lupus Erythematosus
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Lulu Zhang, Fang Du, Qiqi Jin, Li Sun, Boqian Wang, Ziyang Tan, Xinyu Meng, Baozhen Huang, Yifan Zhan, Wenqiong Su, Rui Song, Chunmei Wu, Luonan Chen, Xiaoxiang Chen, and Xianting Ding
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dynamic network biomarker ,mass cytometry ,single‐cell RNA sequencing ,systemic lupus erythematosus ,Science - Abstract
Abstract Systemic Lupus Erythematosus (SLE) etiopathogenesis highlights the contributions of overproduction of CD4+ T cells and loss of immune tolerance. However, the involvement of CD8+ T cells in SLE pathology and disease progression remains unclear. Here, the comprehensive immune cell dysregulation in total 263 clinical peripheral blood samples composed of active SLE (aSLE), remission SLE (rSLE) and healthy controls (HCs) is investigated via mass cytometry, flow cytometry and single‐cell RNA sequencing. This is observed that CD8+CD27+CXCR3− T cells are increased in rSLE compare to aSLE. Meanwhile, the effector function of CD8+CD27+CXCR3− T cells are overactive in aSLE compare to HCs and rSLE, and are positively associated with clinical SLE activity. In addition, the response of peripheral blood mononuclear cells (PBMCs) is monitored to interleukin‐2 stimulation in aSLE and rSLE to construct dynamic network biomarker (DNB) model. It is demonstrated that DNB score‐related parameters can faithfully predict the remission of aSLE and the flares of rSLE. The abundance and functional dysregulation of CD8+CD27+CXCR3− T cells can be potential biomarkers for SLE prognosis and concomitant diagnosis. The DNB score with accurate prediction to SLE disease progression can provide clinical treatment suggestions especially for drug dosage determination.
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- 2023
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21. Celecoxib treatment alleviates cerebral injury in a rat model of post-traumatic epilepsy
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Lei Chen, Qingsheng Niu, Caibin Gao, and Fang Du
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Post-traumatic epilepsy ,Celecoxib ,Cerebral injury ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
Background An important factor contributing to the development and occurrence of post-traumatic epilepsy (PTE) is neuroinflammation and oxidative stress. The effects of celecoxib include inhibiting inflammatory reactions and antioxidant stress and reducing seizures, making it a potential epilepsy treatment solution. Objective To observe the effect of celecoxib on early epilepsy in post-traumatic epilepsy rats. Methods: Twenty-four adult healthy male Sprague-Dawley rats were randomly assigned to three groups: sham-operated, PTE, and celecoxib. A rat model of PTE was established by injecting ferrous chloride into the right frontal cortex. Afterward, the behavior of rats was observed and recorded. 3.0T superconducting magnetic resonance imaging (MRI) was used to describe the changes in ADC values of the brain. HE and Nissl staining were also used to detect the damage to frontal lobe neurons. Furthermore, the expression of COX-2 protein in the right frontal lobe was detected by Western blot. Moreover, the contents of IL-1 and TNF-α in the right frontal lobe were detected by enzyme-linked immunosorbent assay. Results Compared with the PTE group, the degree of seizures in rats treated with celecoxib declined dramatically (P < 0.05). Celecoxib-treated rats had significant decreases in tissue structural damage and cell death in the brain. The results of the MRI showed that celecoxib reduced the peripheral edema zone and ADC value of the cortex around the damaged area of the right frontal lobe in the celecoxib-treatment group, which was significantly decreased compared with the PTE group (P < 0.05). Furthermore, celecoxib decreased the expression of COX-2, IL-1β, and TNF-α in brain tissue (P < 0.05). Conclusions In PTE rats, celecoxib significantly reduced brain damage and effectively reduced seizures. As a result of celecoxib’s ability to inhibit inflammation, it can reduce the edema caused by injury in rat brain tissue.
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- 2023
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22. Strong positive light chain immunostaining in a patient with transthyretin amyloidosis
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Jiao Chen, Haifei Chen, Lingyun Zhou, Danbo Liu, Fang Du, and Hongxian Xiang
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Amyloid transthyretin ,gene sequencing ,immunohistochemistry ,immunoglobulin light chain ,mass spectrometry ,Diseases of the blood and blood-forming organs ,RC633-647.5 - Abstract
ABSTRACTThe two most common systemic amyloidosis types are immunoglobulin light chain (AL) and amyloid transthyretin (ATTR) amyloidosis, in which the precursor proteins responsible for amyloidosis are light chain and transthyretin, respectively. Identification of precursor proteins is paramount to determine the type of amyloidosis, given that both amyloidosis types lack specificity in clinical presentation. Congo red staining followed by immunohistochemistry or immunofluorescence using fibril protein-specific antibodies is crucial for the diagnosis of amyloidosis. Here we describe a patient who was initially diagnosed with AL amyloidosis due to strong positive kappa light chain staining results. However, the diagnosis was corrected to hereditary ATTR amyloidosis using mass spectrometry and gene sequencing, confirming the important role of mass spectrometry in identifying the amyloid precursor protein and ruling out false-positive result from immunohistochemistry.
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- 2023
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23. Acute thrombus formation on the delivery sheath during left atrial appendage occlusion: Case reports with placement of cerebral protection devices
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Yangyang Yu, Fang Du, Feng Zhao, and Hao Hu
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cerebral embolic protection ,left atrial appendage occlusion ,thrombolysis ,thrombus ,Medicine ,Medicine (General) ,R5-920 - Abstract
Key Clinical Message Acute thrombus formation on the delivery sheath is rare condition during percutaneous left atrial appendage occlusion. We presented two cases that transesophageal echocardiography (TEE) showed a floating thrombus attached to the tip of delivery sheath during the procedure. Cerebral embolic protection devices were used to prevent neurological events after thrombus was detected. The neurological function was not impaired post‐procedure.
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- 2023
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24. ShapeFormer: A Shape-Enhanced Vision Transformer Model for Optical Remote Sensing Image Landslide Detection.
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Pengyuan Lv, Lusha Ma, Qiaomin Li, and Fang Du
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- 2023
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25. Analytical Study of the Changes in Brightness Temperature Based on the Tectonic Field Associated With Three Earthquakes in the Eastern Tibetan Plateau.
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Xing Yang, Qian Lu, Tiebao Zhang, Fang Du, Feng Long, Min Zhao, and Xiaofeng Liao
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- 2023
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26. Entanglement concentration of W state using linear optics with a higher success probability.
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Fang-Fang Du, Ming Ma, Xue-Mei Ren, and Gang Fan
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- 2024
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27. Continuation, reduction, or withdrawal of tofacitinib in patients with rheumatoid arthritis achieving sustained disease control: a multicenter, open-label, randomized controlled trial
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Mengyan Wang, Yu Xue, Fang Du, Lili Ma, Liang-jing Lu, Lindi Jiang, Yi-Li Tao, Chengde Yang, Hui Shi, Honglei Liu, Xiaobing Cheng, Junna Ye, Yutong Su, Dongbao Zhao, Sheng-Ming Dai, Jialin Teng, Qiongyi Hu, and Lishao Guo
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Medicine - Abstract
Abstract. Background:. Rheumatoid arthritis (RA), a chronic systemic autoimmune disease, is characterized by synovitis and progressive damage to the bone and cartilage of the joints, leading to disability and reduced quality of life. This study was a randomized clinical trial comparing the outcomes between withdrawal and dose reduction of tofacitinib in patients with RA who achieved sustained disease control. Methods:. The study was designed as a multicenter, open-label, randomized controlled trial. Eligible patients who were taking tofacitinib (5 mg twice daily) and had achieved sustained RA remission or low disease activity (disease activity score in 28 joints [DAS28] ≤3.2) for at least 3 months were enrolled at six centers in Shanghai, China. Patients were randomly assigned (1:1:1) to one of three treatment groups: continuation of tofacitinib (5 mg twice daily); reduction in tofacitinib dose (5 mg daily); and withdrawal of tofacitinib. Efficacy and safety were assessed up to 6 months. Results:. Overall, 122 eligible patients were enrolled, with 41 in the continuation group, 42 in the dose-reduction group, and 39 in the withdrawal group. After 6 months, the percentage of patients with a DAS28-erythrocyte sedimentation rate (ESR) of
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- 2023
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28. Safety and immunogenicity of a mosaic vaccine booster against Omicron and other SARS-CoV-2 variants: a randomized phase 2 trial
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Nawal Al Kaabi, Yun Kai Yang, Yu Liang, Ke Xu, Xue Feng Zhang, Yun Kang, Yu Qin Jin, Jun Wei Hou, Jing Zhang, Tian Yang, Salah Hussein, Mohamed Saif ElDein, Ze Hua Lei, Hao Zhang, Shuai Shao, Zhao Ming Liu, Ning Liu, Xiang Zheng, Ji Guo Su, Sen Sen Yang, Xiangfeng Cong, Yao Tan, Wenwen Lei, Xue Jun Gao, Zhiwei Jiang, Hui Wang, Meng Li, Hanadi Mekki Mekki, Walid Zaher, Sally Mahmoud, Xue Zhang, Chang Qu, Dan Ying Liu, Mengjie Yang, Islam Eltantawy, Peng Xiao, Fu Jie Shen, Jin Juan Wu, Zi Bo Han, Li Fang Du, Fang Tang, Shi Chen, Zhi Jing Ma, Fan Zheng, Ya Nan Hou, Xin Yu Li, Xin Li, Zhao Nian Wang, Jin Liang Yin, Xiao Yan Mao, Jin Zhang, Liang Qu, Yun Tao Zhang, Xiao Ming Yang, Guizhen Wu, and Qi Ming Li
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Medicine ,Biology (General) ,QH301-705.5 - Abstract
Abstract An ongoing randomized, double-blind, controlled phase 2 trial was conducted to evaluate the safety and immunogenicity of a mosaic-type recombinant vaccine candidate, named NVSI-06-09, as a booster dose in subjects aged 18 years and older from the United Arab Emirates (UAE), who had administered two or three doses of inactivated vaccine BBIBP-CorV at least 6 months prior to enrollment. The participants were randomly assigned with 1:1 to receive a booster dose of NVSI-06-09 or BBIBP-CorV. The primary outcomes were immunogenicity and safety against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant, and the exploratory outcome was cross-immunogenicity against other circulating strains. Between May 25 and 30, 2022, 516 adults received booster vaccination with 260 in NVSI-06-09 group and 256 in BBIBP-CorV group. Interim results showed a similar safety profile between two booster groups, with low incidence of adverse reactions of grade 1 or 2. For immunogenicity, by day 14 post-booster, the fold rises in neutralizing antibody geometric mean titers (GMTs) from baseline elicited by NVSI-06-09 were remarkably higher than those by BBIBP-CorV against the prototype strain (19.67 vs 4.47-fold), Omicron BA.1.1 (42.35 vs 3.78-fold), BA.2 (25.09 vs 2.91-fold), BA.4 (22.42 vs 2.69-fold), and BA.5 variants (27.06 vs 4.73-fold). Similarly, the neutralizing GMTs boosted by NVSI-06-09 against Beta and Delta variants were also 6.60-fold and 7.17-fold higher than those by BBIBP-CorV. Our findings indicated that a booster dose of NVSI-06-09 was well-tolerated and elicited broad-spectrum neutralizing responses against divergent SARS-CoV-2 variants, including Omicron and its sub-lineages.
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- 2023
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29. Parallel detection of multiple zoonotic parasites using a real-time fluorogenic loop-mediated isothermal amplification-based quadruple-sample microfluidic chip
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Yu-Xin Chen, Yi-Rong Lou, Li-Jun Duan, Qian-Jin Zhou, Zhong-Jie Xu, Fang-Jie Chen, Hong-Xian Chen, Gui-Zong Xu, Ai-Fang Du, and Jiong Chen
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zoonotic parasite ,microfluidic chip ,loop-mediated isothermal amplification ,low reagent consumption ,multiple detection ,Microbiology ,QR1-502 - Abstract
Zoonotic parasites pose significant health risks globally. In the present study, we combined a microfluidic chip with loop-mediated isothermal amplification (on-chip LAMP) to detect five zoonotic parasites: Toxoplasma gondii, Cryptosporidium parvum, Cryptosporidium hominis, Clonorchis sinensis, and Taenia solium. This method enabled the simultaneous parallel analysis of five genetic markers from a maximum of four samples per chip. The on-chip LAMP assay was conducted in a highly automated format via the addition (by pipetting) of each sample in a single operation. The reaction was performed in volumes as low as 5 μL at a temperature of 65°C for 60 min, achieving limits of detection ranging from 10−2 to 10−3 pg./μL of recombinant plasmid DNA. All the time-to-positive values were less than 40 min, and almost all the coefficients of variation were less than 10%, even when using limit of detection concentrations for multiple pathogens, indicating robust reproducibility among replicates. The clinical sensitivity and specificity for detecting 135 field samples were 98.08 and 97.59%, respectively, compared with traditional biological methods, indicating good applicability in the detection of field samples. This on-chip LAMP assay allows for low reagent consumption, ease of operation, and multiple analyses of samples and genetic targets, and is applicable for on-site detection and the routine monitoring of multiple zoonotic parasites.
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- 2023
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30. A palaeoearthquake event and its age revealed by the travertine layer along the Litang fault in the southeastern margin of the Qinghai-Tibetan plateau
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Yao Yang, Mingjian Liang, Chao Ma, Jun Li, Hualiang Shen, Fang Du, Song Luo, Shao Liu, and Xuelian Rui
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Travertine ,Paleoearthquake ,Soft-sediment deformation ,U-series dating ,Litang fault ,Geophysics. Cosmic physics ,QC801-809 ,Dynamic and structural geology ,QE500-639.5 - Abstract
The Litang fault (LTF), located in the southeast of the Qinghai-Tibetan Plateau, is known for its high level of present-day seismicity, whereas its Pleistocene activity has been scarcely documented. This study focused on a tract of banded travertine deposits precipitated from thermal waters along the NW–SE-trending LTF trace. The role of travertine deposits in recording neotectonic activity has been studied by identifying their internal structure. Typical soft-sediment deformation structures observed within the banded travertines include micro folds, liquefied breccia, and liquefied diapirs. These deformed structures, which are restricted to a single unit separated unconformably by undeformed layers, can be traced for tens of meters, indicating that they were formed by seismic shaking triggered by LTF activity. The deformation of the banded travertine layers is attributed to the combined effects of seismic shaking, liquefaction, and fluidization, and it can be related to a paleo earthquake event with a magnitude of MS > 5. The U-series ages obtained from the banded travertine deposits perturbed by the earthquakes are in the range of 130.59–112.94 ka, indicating an important fault-assisted neotectonic activity that occurred during the Middle–Late Pleistocene. Analysis of such structures, in combination with the use of U-series dating methods, can yield a reliable timing of neotectonic activity and provide new evidence for understanding the seismotectonic setting of the Litang area.
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- 2023
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31. The expression characteristics and clinical significance of ACP6, a potential target of nitidine chloride, in hepatocellular carcinoma
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Li Gao, Dan-Dan Xiong, Xia Yang, Jian-Di Li, Rong-Quan He, Zhi-Guang Huang, Ze-Feng Lai, Li-Min Liu, Jia-Yuan Luo, Xiu-Fang Du, Jiang-Hui Zeng, Ming-Fen Li, Sheng-Hua Li, Yi-Wu Dang, and Gang Chen
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ACP6 ,HCC ,NC ,Xenograft ,RNA-seq ,Microarray ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Acid phosphatase type 6 (ACP6) is a mitochondrial lipid phosphate phosphatase that played a role in regulating lipid metabolism and there is still blank in the clinico-pathological significance and functional roles of ACP6 in human cancers. No investigations have been conducted on ACP6 in hepatocellular carcinoma (HCC) up to date. Methods Herein, we appraised the clinico-pathological significance of ACP6 in HCC via organizing expression profiles from globally multi-center microarrays and RNA-seq datasets. The molecular basis of ACP6 in HCC was explored through multidimensional analysis. We also carried out in vitro and in vivo experiment on nude mice to investigate the effect of knocking down ACP6 expression on biological functions of HCC cells, and to evaluate the expression variance of ACP6 in xenograft of HCC tissues before and after the treatment of NC. Results ACP6 displayed significant overexpression in HCC samples (standard mean difference (SMD) = 0.69, 95% confidence interval (CI) = 0.56–0.83) and up-regulated ACP6 performed well in screening HCC samples from non-cancer liver samples. ACP6 expression was also remarkably correlated with clinical progression and worse overall survival of HCC patients. There were close links between ACP6 expression and immune cells including B cells, CD8 + T cells and naive CD4 + T cells. Co-expressed genes of ACP6 mainly participated in pathways including cytokine-cytokine receptor interaction, glucocorticoid receptor pathway and NABA proteoglycans. The proliferation and migration rate of HCC cells transfected with ACP6 siRNA was significantly suppressed compared with those transfected with negative control siRNA. ACP6 expression was significantly inhibited by nitidine chloride (NC) in xenograft HCC tissues. Conclusions ACP6 expression may serve as novel clinical biomarker indicating the clinical development of HCC and ACP6 might be potential target of anti-cancer effect by NC in HCC.
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- 2022
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32. A cohesin‐associated gene score may predict immune checkpoint blockade in hepatocellular carcinoma
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Cui‐Zhen Liu, Jian‐Di Li, Gang Chen, Rong‐Quan He, Rui Lin, Zhi‐Guang Huang, Jian‐Jun Li, Xiu‐Fang Du, and Xiao‐Ping Lv
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cohesin‐associated gene score ,immunotherapy ,MEGENA ,STAG1 ,TME ,Biology (General) ,QH301-705.5 - Abstract
Stromal antigen 1 (STAG1), a component of cohesion, is overexpressed in various cancers, but it is unclear whether it has a role in the transcriptional regulation of hepatocellular carcinoma (HCC). To test this hypothesis, here, we screened global HCC datasets and performed multiscale embedded gene co‐expression network analysis to identify the potential functional modules of differentially expressed STAG1 co‐expressed genes. The putative transcriptional targets of STAG1 were identified using chromatin immunoprecipitation followed by high‐throughput DNA sequencing. The cohesin‐associated gene score (CAGS) was quantified using the The Cancer Genome Atlas HCC cohort and single‐sample gene set enrichment analysis. Distinct cohesin‐associated gene patterns were identified by calculating the euclidean distance of each patient. We assessed the potential ability of the CAGS in predicting immune checkpoint blockade (ICB) treatment response using IMvigor210 and GSE78220 cohorts. STAG1 was upregulated in 3313 HCC tissue samples compared with 2692 normal liver tissue samples (standard mean difference = 0.54). A total of three cohesin‐associated gene patterns were identified, where cluster 2 had a high TP53 mutated rate and a poor survival outcome. Low CAGS predicted a significant survival advantage but presaged poor immunotherapy response. Differentially expressed STAG1 co‐expression genes were enriched in the mitotic cell cycle, lymphocyte activation, and blood vessel development. PDS5A and PDGFRA were predicted as the downstream transcriptional targets of STAG1. In summary, STAG1 is significantly upregulated in global HCC tissue samples and may participate in blood vessel development and the mitotic cell cycle. A cohesin‐associated gene scoring system may have potential to predict the ICB response.
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- 2022
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33. Gut microbiome changes in anti-N-methyl-D-aspartate receptor encephalitis patients
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Jingya Wei, Xiao Zhang, Fang Yang, Xiaodan Shi, Xuan Wang, Rong Chen, Fang Du, Ming Shi, and Wen Jiang
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Anti-N-Methyl-D-Aspartate Receptor Encephalitis ,Encephalitis ,Gastrointestinal Microbiome ,Gut-brain axis ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Background Anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis is a type of autoimmune encephalitis. The underlying mechanism(s) remain largely unknown. Recent evidence has indicated that the gut microbiome may be involved in neurological immune diseases via the "gut-brain axis". This study aimed to explore the possible relationship between anti-NMDAR encephalitis and the gut microbiome. Methods Fecal specimens were collected from 10 patients with anti-NMDAR encephalitis and 10 healthy volunteers. The microbiome analysis was based on Illumina sequencing of the V3-V4 hypervariable region of the 16S rRNA gene. The alpha, beta, and taxonomic diversity analyses were mainly based on the QIIME2 pipeline. Results There were no statistical differences in epidemiology, medication, and clinical characteristics (except for those related to anti-NMDAR encephalitis) between the two groups. ASV analysis showed that Prevotella was significantly increased, while Bacteroides was reduced in the gut microbiota of the patients, compared with the controls. Alpha diversity results showed a decrease in diversity in the patients compared with the healthy controls, analyzed by the Shannon diversity, Simpson diversity, and Pielou_E uniformity based on the Kruskal–Wallis test (P = 0.0342, 0.0040, and 0.0002, respectively). Beta diversity analysis showed that the abundance and composition of the gut microbiota was significantly different between the two groups, analyzed by weighted and unweighted UniFrac distance (P = 0.005 and 0.001, respectively). Conclusions The abundance and evenness of bacterial distribution were significantly lower and jeopardized in patients with anti-NMDAR encephalitis than in healthy controls. Thus, our findings suggest that gut microbiome composition changes might be associated with the anti-NMDAR encephalitis. It could be a causal agent, or a consequence.
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- 2022
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34. Generating a mouse model for relapsed Sonic Hedgehog medulloblastoma
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Allie Heller, Fang Du, Yongqiang Liu, Yijun Yang, and Zeng-Jie Yang
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Cell Isolation ,Cancer ,Microscopy ,Model Organisms ,Neuroscience ,Science (General) ,Q1-390 - Abstract
Summary: Tumor relapse is the leading adverse prognostic factor in medulloblastoma (MB). However, there is still no established mouse model for MB relapse, impeding our efforts to develop strategies to treat relapsed MB. We present a protocol for generating a mouse model for relapsed MB using irradiation by optimizing mouse breeding and age, as well as irradiation dosage and timing. We then detail procedures for determining tumor relapse based on tumor cell trans-differentiation in MB tissue, immunohistochemistry, and tumor cell isolation.For complete details on the use and execution of this protocol, please refer to Guo et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
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- 2023
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35. rcCAE: a convolutional autoencoder method for detecting intra-tumor heterogeneity and single-cell copy number alterations.
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Zhenhua Yu 0002, Furui Liu, Fangyuan Shi, and Fang Du
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- 2023
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36. Safety and immunogenicity of a hybrid-type vaccine booster in BBIBP-CorV recipients in a randomized phase 2 trial
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Nawal Al Kaabi, Yun Kai Yang, Li Fang Du, Ke Xu, Shuai Shao, Yu Liang, Yun Kang, Ji Guo Su, Jing Zhang, Tian Yang, Salah Hussein, Mohamed Saif ElDein, Sen Sen Yang, Wenwen Lei, Xue Jun Gao, Zhiwei Jiang, Xiangfeng Cong, Yao Tan, Hui Wang, Meng Li, Hanadi Mekki Mekki, Walid Zaher, Sally Mahmoud, Xue Zhang, Chang Qu, Dan Ying Liu, Mengjie Yang, Islam Eltantawy, Jun Wei Hou, Ze Hua Lei, Peng Xiao, Zhao Nian Wang, Jin Liang Yin, Xiao Yan Mao, Jin Zhang, Liang Qu, Yun Tao Zhang, Xiao Ming Yang, Guizhen Wu, and Qi Ming Li
- Subjects
Science - Abstract
SARS-CoV-2 variants with immune escape capability highlight the need for the development of cross-neutralising vaccines and regimens. Here, the authors assess the immunogenicity and safety of NVSI-06-08, that integrates antigens from multiple SARS-CoV-2 strains into a single immunogen, as a heterologous booster in adults previously vaccinated with the inactivated vaccine.
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- 2022
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37. SCViT: A Spatial-Channel Feature Preserving Vision Transformer for Remote Sensing Image Scene Classification.
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Pengyuan Lv, Wenjun Wu, Yanfei Zhong, Fang Du, and Liangpei Zhang 0001
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- 2022
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38. Efficacy and safety of Iguratimod as an add-on therapy for refractory lupus nephritis: A preliminary investigational study
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Qingran Yan, Mei Zhang, Fang Du, Yuening Kang, Ping Ye, Qianqian Li, Bei Liu, Min Dai, and Chunde Bao
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IGU ,refractory lupus nephritis ,combinational therapy ,add-on ,prospective study ,Immunologic diseases. Allergy ,RC581-607 - Abstract
ObjectivesIGU (IGU), a novel immunomodulatory agent for rheumatoid arthritis, has been shown to be effective and safe as monotherapy in a small population with refractory lupus nephritis (LN). The aim of this prospective study was to evaluate the efficacy and safety of IGU as an add-on therapy in patients with refractory LN in the context of clinical practice.MethodsThis is a single-arm observational study. We have enrolled LN patients since 2019 at Renji Hospital. All participants should have recurrent or refractory LN with at least one immunosuppressant (IS) and have a baseline urine protein/creatinine ratio (UPCR) >1.0. After enrollment, we added IGU (25 mg twice daily) to one of their previous immunosuppressants (IS) without increasing the dose of steroids. The primary outcome was the complete renal response (CRR) in the 6th month. UPCR decrease of over 50% was defined as partial response (PR). Extended follow-up was performed after the initial 6 months.ResultsWe enrolled 26 eligible participants. 11/26 patients had chronic kidney disease (CKD) stage 2/3 at the baseline. The IS combined with IGU included mycophenolate mofetil, tacrolimus, and cyclosporin A. No IS change was allowed. 80.7% of patients had baseline steroids less than 0.5mg/kg daily and there was no steroids escalation during the IGU treatment. The CRR rate was 42.3% (11/26) at month 6. With a median follow-up of 52 weeks (range: 23-116 weeks), the CRR rate at the last visit was 50% (13/26) and 73.1% (19/26) of patients had UPCR decrease of over 50%. Six patients withdrew, three for no response and three for renal flare after initial CRR. One patient had an estimated glomerular filtration rate worsening of over 20% and was classified as renal flare. Three mild to moderate adverse events were recorded.ConclusionsOur investigation merits further investigation in IGU as a potentially tolerable component of combination therapy for refractory LN.
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- 2023
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39. Does increasing physician volume in primary healthcare facilities under the hierarchical medical system help reduce hospital service utilisation in China? A fixed-effects analysis using province-level panel data
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Bin Zhu, Xiaotong Li, Pei Xie, Huiwen Xu, Fang Du, Hankun Wang, and Xinxin Han
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Medicine - Abstract
Objective To examine whether increases in physician volume in primary healthcare facilities are associated with reduced utilisation of hospital outpatient and inpatient services after China facilitated the establishment of the hierarchical medical system.Design We used a two-way fixed-effects regression to examine the association between the annual number of physicians in primary healthcare facilities and that of patient visits per physician, inpatient admissions and total expenses per outpatient visit in public hospitals during 2010–2014 and 2015–2019. Variables were log transformed to ensure the normal distribution of the data.Setting Province-level data of all 31 provinces in mainland China from 2010 to 2019 were collected from the China Health Statistics Yearbook published by the China Health Commission.Participants All 31 provinces in mainland China.Primary and secondary outcome measures The annual number of outpatient visits per physician, hospital admission and total expenses per outpatient visit in public hospitals.Results During 2015–2019, we found that, on average, a 1% increase in the number of primary healthcare physicians was accompanied by a 0.19% (95% CI −0.33% to −0.05%) reduction in the annual number of visits per physician in public hospitals, and a 0.31% (95% CI −0.52% to −0.10%) reduction in patient visits in city-administered hospitals. No significant associations were found between 2010 and 2014. We also did not observe any significant associations between primary healthcare physician volume and hospital admissions or outpatient expenses during neither 2010–2014 and 2015–2019.Conclusions In the context of the hierarchical medical system, enhancing physician volume in primary healthcare facilities helps reduce outpatient visits in public hospitals, especially city-administered hospitals. However, more efforts are required to be continuously made to improve primary healthcare capacity to avoid preventable hospital admissions and outpatient expenses.
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- 2023
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40. Hypoxia‐inducible factor‐1α regulates the interleukin‐6 production by B cells in rheumatoid arthritis
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Chaofan Fan, Jia Li, Yixuan Li, Yuyang Jin, Jiaqi Feng, Ruru Guo, Xinyu Meng, Dongcheng Gong, Qian Chen, Fang Du, Chunyan Zhang, Liangjing Lu, Jun Deng, and Xiao‐Xiang Chen
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B cells ,HIF‐1α ,IL‐6 ,rheumatoid arthritis ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Abstract Objectives Rheumatoid arthritis (RA) is a disease characterised by bone destruction and systemic inflammation, and interleukin‐6 (IL‐6) is a therapeutic target for treating it. The study aimed at investigating the sources of IL‐6 and the influence of hypoxia‐inducible factor‐1α (HIF‐1α) on IL‐6 production by B cells in RA patients. Methods The phenotype of IL‐6‐producing cells in the peripheral blood of RA patients was analysed using flow cytometry. Bioinformatics, real‐time polymerase chain reaction, Western blot and immunofluorescence staining were used to determine the IL‐6 production and HIF‐1α levels in B cells. A dual‐luciferase reporter assay and chromatin immunoprecipitation were used to investigate the regulatory role of HIF‐1α on IL‐6 production in human and mouse B cells. Results Our findings revealed that B cells are major sources of IL‐6 in the peripheral blood of RA patients, with the proportion of IL‐6‐producing B cells significantly correlated with RA disease activity. The CD27−IgD+ naïve B cell subset was identified as the typical IL‐6‐producing subset in RA patients. Both HIF‐1α and IL‐6 were co‐expressed by B cells in the peripheral blood and synovium of RA patients, and HIF‐1α was found to directly bind to the IL6 promoter and enhance its transcription. Conclusion This study highlights the role of B cells in producing IL‐6 and the regulation of this production by HIF‐1α in patients with RA. Targeting HIF‐1α might provide a new therapeutic strategy for treating RA.
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- 2023
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41. Design of a mutation-integrated trimeric RBD with broad protection against SARS-CoV-2
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Yu Liang, Jing Zhang, Run Yu Yuan, Mei Yu Wang, Peng He, Ji Guo Su, Zi Bo Han, Yu Qin Jin, Jun Wei Hou, Hao Zhang, Xue Feng Zhang, Shuai Shao, Ya Nan Hou, Zhao Ming Liu, Li Fang Du, Fu Jie Shen, Wei Min Zhou, Ke Xu, Ru Qin Gao, Fang Tang, Ze Hua Lei, Shuo Liu, Wei Zhen, Jin Juan Wu, Xiang Zheng, Ning Liu, Shi Chen, Zhi Jing Ma, Fan Zheng, Si Yu Ren, Zhong Yu Hu, Wei Jin Huang, Gui Zhen Wu, Chang Wen Ke, and Qi Ming Li
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Cytology ,QH573-671 - Abstract
Abstract The continuous emergence of SARS-CoV-2 variants highlights the need of developing vaccines with broad protection. Here, according to the immune-escape capability and evolutionary convergence, the representative SARS-CoV-2 strains carrying the hotspot mutations were selected. Then, guided by structural and computational analyses, we present a mutation-integrated trimeric form of spike receptor-binding domain (mutI-tri-RBD) as a broadly protective vaccine candidate, which combined heterologous RBDs from different representative strains into a hybrid immunogen and integrated immune-escape hotspots into a single antigen. When compared with a homo-tri-RBD vaccine candidate in the stage of phase II trial, of which all three RBDs are derived from the SARS-CoV-2 prototype strain, mutI-tri-RBD induced significantly higher neutralizing antibody titers against the Delta and Beta variants, and maintained a similar immune response against the prototype strain. Pseudo-virus neutralization assay demonstrated that mutI-tri-RBD also induced broadly strong neutralizing activities against all tested 23 SARS-CoV-2 variants. The in vivo protective capability of mutI-tri-RBD was further validated in hACE2-transgenic mice challenged by the live virus, and the results showed that mutI-tri-RBD provided potent protection not only against the SARS-CoV-2 prototype strain but also against the Delta and Beta variants.
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- 2022
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42. Clinical characteristics in the misdiagnosis of cytomegalovirus retinitis: A retrospective analysis of eight patients
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Kui-Fang Du, Xiao-Jie Huang, Chao Chen, Wen-Jun Kong, Lian-Yong Xie, and Wen-Bin Wei
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aids ,cytomegalovirus retinitis ,hiv ,misdiagnosis ,Ophthalmology ,RE1-994 - Abstract
Purpose: To highlight characteristics in the misdiagnosis of cytomegalovirus retinitis (CMVR). Methods: Misdiagnosed cases related to CMVR were analyzed retrospectively at the Department of Ophthalmology, Beijing Youan Hospital, from July 2017 to October 2019. The medical records were reviewed by two independent senior ophthalmologists and the patients' clinical characteristics were analyzed. Results: Eight patients (16 eyes) were identified with misdiagnoses related to CMVR. Six of the patients with CMVR were previously unaware of their human immunodeficiency virus (HIV) infection; one patient with CMVR concealed their history of HIV infection. The cases were initially misdiagnosed as diabetic retinopathy (1/7, 14.3%), branch retinal vein occlusion (1/7, 14.3%), ischemic optic neuropathy (1/7, 14.3%), Behçet's disease (1/7, 14.3%), iridocyclitis (2/7, 28.6%), and progressive outer retinal necrosis (1/7, 14.3%). One patient with binocular renal retinopathy and chronic renal insufficiency was misdiagnosed with CMVR. Four eyes (4/16, 25%) presented with pan-retinal involvement. Fourteen eyes (14/16, 87.5%) had optic disc or macular area involvement. At the final diagnosis, one patient was blind, and two patients had low vision. Seven AIDS patients showed an extremely low level of CD4+ T lymphocytes (median of 5 cells/μl; range 1–9 cells/μl). Conclusion: CMVR may be misdiagnosed in the absence of known immune suppression. CMVR and HIV screening cannot be overlooked if a young male patient presents with yellowish-white retinal lesions. These misdiagnosed patients had severe retinitis associated with poor vision.
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- 2022
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43. Contrast-enhanced ultrasound as a valuable imaging modality for characterizing testicular lesions
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Jie Yu, Xin-Hui Jiang, Lian-Fang Du, Min Bai, Zhao-Jun Li, Qiu-Sheng Shi, Qi Jiang, and Fan Li
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characterization ,contrast-enhanced ultrasound ,diagnosis ,testicular neoplasms ,ultrasound ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Contrast-enhanced ultrasound (CEUS) is a new form of ultrasound (US) that can dynamically display microvessels in a highly sensitive manner. The purpose of this study was to investigate the efficacy of CEUS for characterizing testicular lesions in comparison with conventional US. Forty-seven patients with testicular lesions were enrolled. The histopathology results revealed that 31 cases were neoplastic (11 cases of seminomas, 8 nonseminomatous germ cell tumors, 8 lymphomas, 2 Leydig cell tumors, and 2 nonspecific tumors), and 16 cases were nonneoplastic (8 cases of infarctions, 3 epidermoid cysts, and 5 inflammation). The indicators of shallow lobulated morphology and cystic-solid echogenicity on conventional US were suggestive of germ cell tumors. More indicators on CEUS were found to be useful for characterizing testicular lesions. All the neoplastic lesions showed hyperenhancement on CEUS. Moreover, germ cell tumors presented with heterogeneous enhancement (73.7%, 14/19), a twisted blood vessel pattern, rapid wash-in and wash-out, and peripheral rim hyperenhancement signs. Lymphoma was characterized by nonbranching linear vessel patterns (87.5%, 7/8), rapid wash-in and slow wash-out. In nonneoplastic lesions, infarction and epidermoid cysts showed no enhancement, and abscesses were observed with marginal irregular enhancement. The sensitivity, specificity, and accuracy of CEUS for differentiating between neoplastic and nonneoplastic lesions were 100%, 93.8%, and 97.9%, respectively, and these values were higher than those for conventional US (90.3%, 62.5%, and 80.9%, respectively). CEUS can sensitively reflect the microvascular perfusion in testicular lesions and offers high accuracy for characterizing them.
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- 2022
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44. Spatial Diffusion Characteristics of Tibetan Population in Inland China from 1953 to 2010 - A Spatial Visualization Method Based on ArcGIS.
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Linghua Zhang, Fang Du, and Jingjin Zhu
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- 2021
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45. Enhanced Bayesian detection for copy number alterations from next-generation sequencing data.
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Zhenhua Yu 0002 and Fang Du
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- 2021
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46. AMC: accurate mutation clustering from single-cell DNA sequencing data.
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Zhenhua Yu 0002 and Fang Du
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- 2022
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47. Network Architecture Reasoning Via Deep Deterministic Policy Gradient.
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Huidong Liu, Fang Du, Xiaofen Tang, Hao Liu 0019, and Zhenhua Yu 0002
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- 2020
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48. Joint Learning with Pre-trained Transformer on Named Entity Recognition and Relation Extraction Tasks for Clinical Analytics.
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Miao Chen, Ganhui Lan, Fang Du, and Victor S. Lobanov
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- 2020
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49. Depletion of high-content CD14+ cells from apheresis products is critical for successful transduction and expansion of CAR T cells during large-scale cGMP manufacturing
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Xiuyan Wang, Oriana Borquez-Ojeda, Jolanta Stefanski, Fang Du, Jinrong Qu, Jagrutiben Chaudhari, Keyur Thummar, Mingzhu Zhu, Ling-bo Shen, Melanie Hall, Paridhi Gautam, Yongzeng Wang, Brigitte Sénéchal, Devanjan Sikder, Prasad S. Adusumilli, Renier J. Brentjens, Kevin Curran, Mark B. Geyer, Sham Mailankhody, Roisin O’Cearbhaill, Jae H. Park, Craig Sauter, Susan Slovin, Eric L. Smith, and Isabelle Rivière
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CAR T cell ,cGMP ,clinical grade ,large-scale manufacturing ,monocyte depletion ,Genetics ,QH426-470 ,Cytology ,QH573-671 - Abstract
With the US Food and Drug Administration (FDA) approval of four CD19- and one BCMA-targeted chimeric antigen receptor (CAR) therapy for B cell malignancies, CAR T cell therapy has finally reached the status of a medicinal product. The successful manufacturing of autologous CAR T cell products is a key requirement for this promising treatment modality. By analyzing the composition of 214 apheresis products from 210 subjects across eight disease indications, we found that high CD14+ cell content poses a challenge for manufacturing CAR T cells, especially in patients with non-Hodgkin’s lymphoma and multiple myeloma caused by the non-specific phagocytosis of the magnetic beads used to activate CD3+ T cells. We demonstrated that monocyte depletion via rapid plastic surface adhesion significantly reduces the CD14+ monocyte content in the apheresis products and simultaneously boosts the CD3+ content. We established a 40% CD14+ threshold for the stratification of apheresis products across nine clinical trials and demonstrated the effectiveness of this procedure by comparing manufacturing runs in two phase 1 clinical trials. Our study suggests that CD14+ content should be monitored in apheresis products, and that the manufacturing of CAR T cells should incorporate a step that lessens the CD14+ cell content in apheresis products containing more than 40% to maximize the production success.
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- 2021
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50. Impact of immune checkpoint molecules on FoxP3+ Treg cells and related cytokines in patients with acute and chronic brucellosis
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Hua-Li Sun, Xiu-Fang Du, Yun-Xia Tang, Guo-Qiang Li, Si-Yuan Yang, Ling-Hang Wang, Xing-Wang Li, Cheng-Jie Ma, and Rong-Meng Jiang
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Brucellosis ,Acute infection ,Chronic infection ,Cytokine ,Tregs ,PD-1 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background The immunoregulatory functions of regulatory T cells (Tregs) in the development and progression of some chronic infectious diseases are mediated by immune checkpoint molecules and immunosuppressive cytokines. However, little is known about the immunosuppressive functions of Tregs in human brucellosis, which is a major burden in low-income countries. In this study, expressions of immune checkpoint molecules and Treg-related cytokines in patients with acute and chronic Brucella infection were evaluated to explore their impact at different stages of infection. Methods Forty patients with acute brucellosis and 19 patients with chronic brucellosis admitted to the Third People’s Hospital of Linfen in Shanxi Province between August 2016 and November 2017 were enrolled. Serum and peripheral blood mononuclear cells were isolated from patients before antibiotic treatment and from 30 healthy subjects. The frequency of Tregs (CD4+ CD25+ FoxP3+ T cells) and expression of CTLA-4, GITR, and PD-1 on Treg cells were detected by flow cytometry. Levels of Treg-related cytokines, including IL-35, TGF-β1, and IL-10, were measured by customised multiplex cytokine assays using the Luminex platform. Results The frequency of Tregs was higher in chronic patients than in healthy controls (P = 0.026) and acute patients (P = 0.042); The frequency of CTLA-4+ Tregs in chronic patients was significantly higher than that in healthy controls (P = 0.011). The frequencies of GITR+ and PD-1+ Tregs were significantly higher in acute and chronic patients than in healthy controls (P 0.05). Serum TGF-β1 levels were higher in chronic patients (P = 0.029) and serum IL-10 levels were higher in acute patients (P = 0.033) than in healthy controls. We detected weak correlations between serum TGF-β1 levels and the frequencies of Tregs (R = 0.309, P = 0.031) and CTLA-4+ Tregs (R = 0.302, P = 0.035). Conclusions Treg cell immunity is involved in the chronicity of Brucella infection and indicates the implication of Tregs in the prognosis of brucellosis. CTLA-4 and TGF-β1 may contribute to Tregs-mediated immunosuppression in the chronic infection stage of a Brucella infection.
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- 2021
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