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1. Unscreened Coulomb repulsion in the one dimensional electron gas

Author

Fano, G., Ortolani, F., Parola, A., and Ziosi, L.

Subjects
Condensed Matter - Strongly Correlated Electrons
Abstract

A tight binding model of electrons interacting via bare Coulomb repulsion is numerically investigated by use of the Density Matrix Renormalization Group method which we prove applicable also to very long range potentials. From the analysis of the elementary excitations, of the spin and charge correlation functions and of the momentum distribution, a picture consistent with the formation of a one dimensional "Wigner crystal" emerges, in quantitative agreement with a previous bosonization study. At finite doping, Umklapp scattering is shown to be ineffective in the presence of long range forces., Comment: RevTex, 5 pages with 8 eps figures. To be published on Phys. Rev. B

Published
1999
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2. Density matrix renormalization group study of dimerization of the Pariser-Parr-Pople model of polyacetilene

Author

Bendazzoli, G. L., Evangelisti, S., Fano, G., Ortolani, F., and Ziosi, L.

Subjects
Condensed Matter - Strongly Correlated Electrons
Abstract

We apply the DMRG method to the Pariser-Parr-Pople hamiltonian and investigate the onset of dimerization. We deduce the parameters of the hopping term and the contribution of the sigma bonds from ab initio calculations on ethylene. Denoting by R_{ij} the C-C distances, we perform a variational optimization of the dimerization delta= (R_{i,i+1} - R_{i-1,i})/2 and of the average bond length R_0 for chains up to N=50 sites. The critical value of N at which the transition occurs is found to be between N=14 and N=18 for the present model. The asymptotic values for large N for R_0 and delta are given by 1.408(3) angstroms and 0.036(0) angstroms., Comment: 7 pages, Latex (RevTex) with 7 eps figures, to be published in the Journal of Chemical Physics

Published
1998
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3. The density matrix renormalization group method. Application to the PPP model of a cyclic polyene chain

Author

Fano, G., Ortolani, F., and Ziosi, L.

Subjects
Condensed Matter - Strongly Correlated Electrons
Abstract

The density matrix renormalization group (DMRG) method introduced by White for the study of strongly interacting electron systems is reviewed; the method is variational and considers a system of localized electrons as the union of two adjacent fragments A, B. A density matrix rho is introduced, whose eigenvectors corresponding to the largest eigenvalues are the most significant, the most probable states of A in the presence of B; these states are retained, while states corresponding to small eigenvalues of rho are neglected. It is conjectured that the decreasing behaviour of the eigenvalues is gaussian. The DMRG method is tested on the Pariser-Parr-Pople Hamiltonian of a cyclic polyene (CH)_N up to N=34. A Hilbert space of dimension 5 x 10^+18 is explored. The ground state energy is 10^-3 eV within the full CI value in the case N=18. The DMRG method compares favourably also with coupled cluster approximations. The unrestricted Hartree-Fock solution (which presents spin density waves) is briefly reviewed, and a comparison is made with the DMRG energy values. Finally, the spin-spin and density-density correlation functions are computed; the results suggest that the antiferromagnetic order of the exact solution does not extend up to large distances but exists locally. No charge density waves are present., Comment: 8 pages, RevTex, 2 figures, to be published in the Journal of Chemical Physics

Published
1998
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4. Numerical results for generalized RVB wavefunctions

Author

Fano, G., Ortolani, F., and Ziosi, L.

Subjects
Condensed Matter
Abstract

Numerical results are presented for a generalized resonance valence bond state which includes both ionic and covalent contributions in each bond ; non nearest-neighbor bonds are also considered. Variational calculations have been performed and the space group symmetry has been taken into account. The results for the Hubbard Hamiltonian are compared with the exact ones for the $ 2 X 2 X 2 $ and the $ 4 X 4 $ lattices. The agreement is quite satisfactory for large values of the interaction U ( for U=40 the overlap with the exact ground state wave function is 0.999 ). Several correlation functions are also compared., Comment: Plain Tex, 21 pages, to be published in Phys. Rev.B

Published
1996

5. Abstract

Author

Andries, L. J., Sys, S. U., Meulemans, A. L., Schuurkes, J. A. J., Vanheel, B., Van de Voorde, J., De Smet, P., Li, J., Van Driessche, W., Flamion, B., Foulon, S., Abramow, M., Calders, P., Eechaute, W., Lacroix, E., Weyne, I., Hoeben, D., Burvenich, C., Fransen, P., Andries, L. J., Van Bedaf, D., Demolder, M., Sys, S. U., Ouedraogo, R., Lebrun, P., Herchuelz, A., Antoine, M. -H., Vandenput, S., Votion, D., Anciaux, N., Duvivier, D. H., Art, T., Lekeux, P., Votion, D., Ghafir, Y., Vandenput, S., Art, T., Lekeux, P., Geurts, M., Hermans, E., Maloteaux, J. -M., Perrad, B., Noel, B., Lagache, F., Bister, J. L., Paquay, R., Bister, J. L., Derycke, G., Vandermeir, M. A., Paquay, R., de Brouwer, S., Porret, C. -A., Stergiopulos, N., Meister, J. -J., Verbeke, M., Van de Voorde, J., Lameire, N., De Clerck, N. M., De Schuytter, J., Tytgato, J., Buyse, G., Eggermont, J., Droogmans, G., Nilius, B., Daenens, P., Salomone, S., Feron, O., Dessy, C., Morel, N., Godfraind, T., Aloisi, A. M., Sacerdote, P., Lodi, L., Carli, G., Carobi, C., Garinei, G., Miniaci, U. C., Scotto, P., Sabatino, M., Sardo, P., Iurato, L., La Grutta, V., Bagni, M. A., Cecchi, G., Cecchini, E., Colomo, F., Garzella, P., Bottinelli, R., Harridge, S. D. H., Canepari, M., Reggiani, C., Reggiani, Saltin, Bambagioni, D., Fanò, G., Menchetti, G,, Danieli-Betto, P., Esposito, A., Betto, R., Megighian, A., Midrio, M., Betto, D. Danieli, Esposito, A., Megighian, A., Midrio, M., Orizio, C., Liberati, D., Locatelli, C., De Grandis, D., Veicsteinas, A., Angoli, D., Delia, D., Wanke, E., Bramucci, M., Miano, A., Quassinti, L., Maccari, E., Murri, O., Amici, D., Cibelli, G., Jüngling, S., Schoch, S., Gerdest, H. H., Thiel, G., Demori, I., Bottazzi, C., Voci, A., Fugassa, E., Barreca, A., Minuto, F., Gallo, G., Fulle, S., Belia, S., Menchetti, G., Cacchio, M., Fanò, G., Gastaldi, G., Laforenza, U., Ferrari, G., Rindi, G., Doni, M. G., Padoin, E., Residori, O., Cesaro, M., Toma, L., Rubini, A., Mutinelli, F, Paulesu, L., Romagnolie, R., Cintorino, M., Pippia, P., Meloni, M. A., Sciola, L., Spano, A., Cogoli-Greuter, M., Cogoli, A., Sardini, A., Mintenig, G. M., Valverde, M. A., Sepùlveda, F. V., Gill, D. R., Hyde, S. C., Higgins, C. F., McNaughton, P. A., Spena, A., Arcuri, M. T., Bonofiglio, S., Chimenti, R., Covello, C., De Cicco, T., Mazzulla, S., Martino, G., Tottene, A., Moretti, A., Pietrobon, D., Baccari, M. C., Calamai, F., Staderini, G., Cova, E., Marelli, R., Sommi, P., Ventura, U., Lombardi, A. M., Fabris, R., Pagano, C., Federspil, G., Vettor, R., Mancinelli, R., Tonali, P., Servidei, S., Romani, R., Tringali, A., Azzena, G. B., Mulè, F., Serio, R., Postorino, A., Pagano, C., Rizzato, M., Granzotto, M., Lombardi, A. M., Fabris, R., Vettor, R., Federspil, G., Sommi, P., Ricci, V., Romano, M., Ivey, K. J., Ventura, U., Vacca, G., Papillo, B., Mary, D. A. S. G., Battaglia, A., Grossini, E., Vacca, G., Papillo, B., Battaglia, A., Grossini, E., Accili, E. A., Redaelli, G., DiFrancesco, D., Antoniotti, S., Distasi, C., Lovisolo, D., Munaron, L., Bertaso, F., Assandri, R., Mazzanti, M., Bianchi, L., Arcangeli, A., Faravelli, L., Becchetti, A., Coronello, M., Mini, E., Francini, F., Olivotto, M., Wanke, E., Bigiani, A., Kim, D. -J., Roper, S. D., Carabelli, V., Lovallo, M., Magnelli, V., Zucker, H., Carbone, E., D’Angelo, E., Rossi, P., De Filippi, G., Taglietti, V., Faravelli, L., Bianchi, L., Arcangeli, A., Francini, F., Olivotto, M., Wanke, E., Francini, F., Bencini, C., Squecco, R., Guatteo, E., Bacci, A., Franceschetti, S., Avanzini, G., Wanke, E., Magnelli, V., Carbone, E., Mazzanti, M., Assandri, R., Ferroni, A., DiFrancesco, D., Navangione, A., Vellani, V., Rispoli, G., Peres, A., Centinaio, E., Giovannardi, S., Russo, G., Marcotti, W., Prigioni, I., Trequattrini, C., Harper, A. A., Petris, A., Franciolini, F., Zaza, A., Micheletti, M., Brioschi, A., Antonutto, G., Capelli, C., Girardis, M., Zamparo, P., di Prampero, P. E., Antonutto, G., Girardis, M., Tuniz, D., di Prampero, P. E., Filippi, G. M., Troiani, D., Grassi, B., Poole, D. C., Richardson, R. S., Knight, D. R., Erickson, B. K., Wagner, P. D., Aimi, B., Stilli, D., Gallo, P., Sgoifo, A., Lagrasta, C., Olivetti, G., Reali, N., Casti, A., Musso, E., Alloatti, G., Penna, C., Gallo, M. P., Levi, R. C., Fenoglio, I., Appendino, G., Gallo, P., Sgoifo, A., Medici, D., Aimi, B., Manghi, M., Stilli, D., Musso, E., Sgoifo, A., Pasini, E., Gallo, P., Aimi, B., Stilli, D., Ceconi, C., Musso, E., Baldissera, F., Cavallari, P., Locatelli, M., Bartesaghi, R., Gessi, T., Benfenati, F., Valtorta, F., Onofri, F., Poo, M., Greengard, P., Biagini, G., Sala, D., Viani§, P., Kozlov, A. V., Zini, I., Bravin, M., Tempia, F., Strata, P., Brescia, G., Di Benedetto, C., Corsi, P., Cangiano, G., Buttiglione, M., Ambrosini, M., Gennarini, G., Casadio, A., Levi, R. C., Montarolo, P. G., Cesare, P., Stoughton, R., McNaughton, P. A., D’Arcangelo, G., Dodt, H. U., Brancati, A., Zieglgänsberger, W., Errico, P., Ferraresi, A., Barmack, N. H., Pettorossi, V. E., Gasparini, S., D’Ambrosio, R., Janigro, D., DiFrancesco, D., Gritti, I., Marintti, M., Calcaterra, R., Freddi, R., Mancia, M., Imeri, I., Bianchi, S., Mancia, M., Lui, F., Gregory, K. M., Blanks, R. H. I., Giolli, R. A., Benassi, C., Corazza, R., Magherini, P. C., Bardoni, R., Belluzzi, O., Mancinelli, R., Manni, E., Azzena, G. B., Tringali, A., Romani, R., Diana, M., Fratta, W., Manzoni, D., Andre, P., Pompeiano, O., Mazzocchio, R., Rossi, A., Melis, F., Kitura, A., Caria, M. A., Asanuma, H., Melone, M, De Biasi, S, Minelli, A, Conti, F, Minelli, A, Karschin, C, DeBiasi, S, Brecha, N C, Conti, F, Monda, M., Amaro, S., Sullo, A., De Luca, B., Monda, M., Amaro, S., Sullo, A., De Luca, B., Pantò, M. R., Cicirata, F., Parenti, R., Serapide, M. F., Parenti, R., Wassef, M., Cicirata, F., Podda, M. V., Solinas, A., Chessa, G., Deriu, F., Mameli, O., Tolu, E., Pompeiano, O., Andre, P., Manzoni, D., Porro, C. A., Francescato, M. P., Cettolo, V., Diamond, M. E., Baraldi, P., Bazzocchi, M., Rivosecchi, R., Konnerth, A., Rossi, M. L., Martini, M., Pelucchi, B., Fesce, R., Santarelli, L., Schacher, S., Montarolo, P. G., Santarelli, R., Grassi, C., Valente, A., Nisticò, S., Bagetta, G., Azzena, G. B., Scuri, Rossana, Garcia-Gil, Mercedes, Mozzachiodi, Riccardo, Brunelli, Marcello, Stefani, G., Onofri, F., Vaccaro, P., Nielander, H. B., Benfenati, F., Tancredi, V., D’Antuono, M., Siniscalchi, A., Brancati, A., Avoli, M., Vellani, V., Navangione, A., Rispoli, G., Verzè, L., Buffo, A., Rossi, F., Oestreicher, A. B., Gispen, W. H., Strata, P., Zamboni, G., Amici, R., Jones, C. A., Perez, E., Domeniconi, R., Parmeggiani, P. L., Zoli, Michele, Le Novàre, Nicolas, Changeux, Jean -Pierre, Lafortuna, C. L., Reinach, E., Saibene, F., Scotto, P., Zocchi, L., Agostoni, E., and Cremaschi, D.

Published
1996
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17. The effect of simulated microgravity on the osteo-articular system: an in vitro study of long-term culture of cartilage and bone tissue explants in the RCCSTM bioreactor

Author

Mazzoleni, G., Steimberg, N., Cosmi, Francesca, Dreossi, D., Bizzarri, M., Mariggiò, M. A., Fanò, G., ISGP - ELGRA, Mazzoleni, G., Steimberg, N., Cosmi, Francesca, Dreossi, D., Bizzarri, M., Mariggiò, M. A., and Fanò, G.

Subjects
osteo-articular system, simulated microgravity, RCCSTM bioreactor, Cell Method, Numerical model
Abstract

Long-term spaceflight affects almost all physiological systems in humans and considerable amount of data revealed its serious impact on skeletal homeostasis. While a microgravity environment has been proved to induce significant mineral loss and bone fragility (affecting, specifically, cancellous weight-bearing bones), its effect on articular cartilage (AC) is poorly known. AC is an avascular tissue, composed of relatively few mechanosensitive cells (chondrocytes), that synthesize a mechanically functional extracellular matrix (ECM), composed of collagen, proteoglycans and other proteins. In response to physical factors (e.g. pressure and deformation) chondrocytes regulate AC histomorphology and function, and may affect bone tissue homeostasis. It is then likely that the absence of gravitational load should alter chondrocytes’ activity. In the present study we investigated, in vitro, the effect of long-term exposure to a simulated microgravity condition (vector-averaged gravity) on whole explants of cancellous bone (rat tibial proximal epiphyses) and AC tissue (newborn rabbit knee’s joint). Tissue explants were kept in culture for up to 4 weeks by the use of the Rotary Cell Culture System (RCCSTM) bioreactor, the unique device, operating on the Earth’s surface, capable of successfully simulate a microgravity environment. The analysis of the structural/mechanical properties of cancellous bone explants was performed by a numerical model based on the Cell Method, applied to the 3D reconstruction of micro-computed tomography scans of the bone samples. With regard to AC tissue, functional and structural properties were studied by comparative cell viability, histochemical and molecular analyses performed on either the cellular component or on their ECM. The results obtained demonstrate that, while our RCCSTM-based culture system is able to preserve native tissue architecture and cells’ viability all during the experimental procedure, long-term exposure to a microgravity environment may effectively alter bone cells’ and articular chondrocytes’ physiology.

Published
2010

18. Cellular and molecular responses of human skeletal muscle exposed to hypoxic environment

Author

Mancinelli, R., Pietrangelo, T., La Rovere, R., Luana Toniolo, Fanò, G., Carlo Reggiani, and Fulle, S.

Subjects
Adult, Male, Satellite Cells, Skeletal Muscle, Altitude, Proteolysis, Humans, Regeneration, Middle Aged, Hypoxia, Muscle, Skeletal, Adaptation, Physiological, Exercise
Abstract

The effects of a hypobaric, hypoxic environment and exercise performed under extreme conditions, such as at high altitudes, are intriguing physiological aspects that need to be investigated directly on human climbers. Their skeletal muscle is one of the main tissues that can suffer from hypoxia and physical challenges, which will both define the muscle adaptation and the molecular signature of regenerative capacity. We investigated the muscle regenerative capacity characterizing satellite cells. Our study shows that satellite cells are altered by hypobaric, hypoxic environments and exercise performed at high altitudes. Of note, in human skeletal muscle after this 5,000 m a.s.l. expedition, SCs showed a significantly lower ability to regenerate skeletal muscle, in respect to before this high-altitude expedition. This impairment appears to be due to reduced satellite cell activity, consistent with their decreased myogenicity and fusion ability. Furthermore, at the transcriptional level several pathways, such as cell cycle, myogenesis, oxidative metabolism, proteolysis and sarcomeric protein synthesis, were found dysregulated.

Published
2011

22. Skeletal Muscle Is a Primary Target of SOD1G93A-Mediated Toxicity

Author

Dobrowolny G. 1, Aucello M. 1, Rizzuto E. 1, Beccafico S. 2, Mammucari C.3, Bonconpagni S.2, Belia S.3, Wannenes F. 4, NicolettiC. 1, Del Prete Z. 5, Rosenthal N.6, Molinaro M. 1, Protasi F.2, Fanò G. 2, Sandri M. 7, 8, 9, and Musarò A.1

Abstract

The antioxidant enzyme superoxide dismutase 1 (SOD1) is a critical player of the antioxidative defense whose activity is altered in several chronic diseases, including amyotrophic lateral sclerosis. However, how oxidative insult affects muscle homeostasis remains unclear. This study addresses the role of oxidative stress on muscle homeostasis and function by the generation of a transgenic mouse model expressing a mutant SOD1 gene (SOD1G93A) selectively in skeletal muscle. Transgenic mice developed progressive muscle atrophy, associated with a significant reduction in muscle strength, alterations in the contractile apparatus, and mitochondrial dysfunction. The analysis of molecular pathways associated with muscle atrophy revealed that accumulation of oxidative stress served as signaling molecules to initiate autophagy, one of the major intracellular degradation mechanisms. These data demonstrate that skeletal muscle is a primary target of SOD1G93A -mediated toxicity and disclose the molecular mechanism whereby oxidative stress triggers muscle atrophy.

Published
2008

24. Age and sex influence on oxidative damage and functional status in human skeletal muscle

Author

Fanò, G., Mecocci, P., Vecchiet, J., Belia, S., Fulle, S., Polidori, M. C., Felzani, G., Senin, U., Vecchiet, L., and Beal, M. F.

Subjects
Adult, Aged, 80 and over, Male, Aging, Sex Characteristics, Adolescent, Secale, Oxidative Stress, Sex Factors, Humans, Regression Analysis, Female, Ca(2+) Mg(2+)-ATPase, Muscle, Skeletal, Aged
Abstract

A reduction in muscle mass, with consequent decrease in strength and resistance, is commonly observed with advancing age. In this study we measured markers of oxidative damage to DNA, lipids and proteins, some antioxidant enzyme activities as well Ca2+ transport in sarcoplasmic reticulum membranes in muscle biopsies from vastus lateralis of young and elderly healthy subjects of both sexes in order to evaluate the presence of age- and sex-related differences. We found a significant increase in oxidation of DNA and lipids in the elderly group, more evident in males, and a reduction in catalase and glutathione transferase activities. The experiments on Ca2+ transport showed an abnormal functional response of aged muscle after exposure to caffeine, which increases the opening of Ca2+ channels, as well a reduced activity of the Ca2+ pump in elderly males. From these results we conclude that oxidative stress play an important role in muscle aging and that oxidative damage is much more evident in elderly males, suggesting a gender difference maybe related to hormonal factors.

Published
2002

31. VO2 uptake kinetics and shift in muscle fiber type in Improved high-altitude trekkers.

Author

Doria, C., Toniolo, L., Verratti, V., Cancellara, P., Pietrangelo, T., Marconi, V., Paoli, A., Pogliaghi, S., Fanò, G., Reggiani, C., and Capelli, C.

Subjects
PHYSIOLOGICAL effects of oxygen, OXYGEN in the body, HIKERS, HUMAN mechanics, EXERCISE physiology
Abstract

The study investigated the effect of prolonged hypoxia on central [i.e., cardiovascular oxygen delivery (QaO2)] and peripheral (i.e., O2 utilization) determinants of oxidative metabolism response during exercise in humans. To this aim, seven male mountaineers were examined before and immediately after the Himalayan Expedition Interamnia 8000-Manaslu 2008, lasting 43 days, among which, 23 days were above 5,000 m. The subjects showed a decrease in body weight (P < 0.05) and of power output during a Wingate Anaerobic test (P < 0.05) and an increase of thigh cross-sectional area (P < 0.05). Absolute maximal O2 uptake (VO2max) did not change. The mean response time of 402 kinetics at the onset of step submaximal cycling exercise was reduced significantly from 53.8 s ± 10.9 to 39.8 s ± 10.9 (P < 0.05), whereas that of QaO2 was not. Analysis of single fibers dissected from vastus lateralis biopsies revealed that the expression of slow isoforms of both heavy and light myosin subunits increased, whereas that of fast isoforms decreased. Unloaded shortening velocity of fibers was decreased significantly. In summary, independent findings converge in indicating that adaptation to chronic hypoxia brings about a fast-to-slow transition of muscle fibers, resulting in a faster activation of the mitochondrial oxidative metabolism. These results indicate that a prolonged and active sojourn in hypoxia may induce muscular ultrastructural and functional changes similar to those observed after aerobic training. [ABSTRACT FROM AUTHOR]

Published
2011
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33. Extracellular guanosine and GTP promote expression of differentiation markers and induce S-phase cell-cycle arrest in human SH-SY5Y neuroblastoma cells

Author

Guarnieri, S., Pilla, R., Morabito, C., Sacchetti, S., Mancinelli, R., Fanò, G., and Mariggiò, M.A.

Subjects
GUANOSINE triphosphate, BIOMARKERS, GENE expression, NEUROBLASTOMA
Abstract

Abstract: SH-SY5Y neuroblastoma cells, a model for studying neuronal differentiation, are able to differentiate into either cholinergic or dopaminergic/adrenergic phenotypes depending on media conditions. Using this system, we asked whether guanosine (Guo) or guanosine-5′-triphosphate (GTP) are able to drive differentiation towards one particular phenotype. Differentiation was determined by evaluating the frequency of cells bearing neurites and assessing neurite length after exposure to different concentrations of Guo or GTP for different durations. After 6 days, 0.3mM Guo or GTP induced a significant increase in the number of cells bearing neurites and increased neurite length. Western blot analyses confirmed that purines induced differentiation; cells exposed to purines showed increases in the levels of GAP43, MAP2, and tyrosine hydroxylase. Proliferation assays and cytofluorimetric analyses indicated a significant anti-proliferative effect of purines, and a concentration-dependent accumulation of cells in S-phase, starting after 24h of purine exposure and extending for up to 6 days. A transcriptional profile analysis using gene arrays showed that an up-regulation of cyclin E2/cdk2 evident after 24h was responsible for S-phase entry, and a concurrent down-regulation of cell-cycle progression-promoting cyclin B1/B2 prevented S-phase exit. In addition, patch-clamp recordings revealed that 0.3mM Guo or GTP, after 6 day incubation, significantly decreased Na+ currents. In conclusion, we showed Guo- and GTP-induced cell-cycle arrest in neuroblastoma cells and suggest that this makes these cells more responsive to differentiation processes that favor the dopaminergic/adrenergic phenotype. [Copyright &y& Elsevier]

Published
2009
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34. Transcriptional Profile of Denervated Vastus LateralisMuscle Derived from a Patient 8 Months after Spinal Cord Injury: A Case Report

Author

Mancinelli, R., Kern, H., Fulle, S., Carraro, U., Zampieri, S., La Rovere, R., Fanò, G., and Pietrangelo, T.

Abstract

A lack of motor neurons abolishes both neurotrophic factor secretion and contractile activity in muscle, which impairs mass, contractile properties, and fibre-type characteristics of the muscle. However, the molecular pathways that can be stimulated or repressed in the scenario of spinal cord injury remain unknown. We investigated for the first time the transcriptional profile of a young male patient 8 months after spinal cord injury. Adaptive metabolic changes of complete denervated skeletal muscle were revealed. In particular, the main molecular pathways involved include metabolic and proteolitic pathways, mitochondrial and synaptic function, calcium homeostasis, sarcomere and anchorage structures. Our data depict the molecular signalling still present in complete denervated skeletal muscle fibres a few months after spinal cord injury. These data could be of interest also to design a specific therapeutic approach aimed at the electrical-stimulation of severe atrophied skeletal muscle.

Published
2011
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38. Transcription Profile Analysis of Vastus LateralisMuscle from Patients with Chronic Fatigue Syndrome

Author

Pietrangelo, T., Mancinelli, R., Toniolo, L., Montanari, G., Vecchiet, J., Fanò, G., and Fulle, S.

Abstract

Chronic fatigue syndrome (CFS) is a disabling condition characterized by unexplained chronic fatigue that impairs normal activities. Many body systems are affected and etiology has not yet been identified. In addition to immunological and psychological aspects, skeletal muscle symptoms are prominent in CFS patients. In an effort to establish which pathways might be involved in the onset and development of muscle symptoms, we used global transcriptome analysis to identify genes that were differentially expressed in vastus lateralismuscle of female and male CFS patients. We found that the expression of genes that play key roles in mitochondrial function and oxidative balance, including superoxide dismutase 2, were altered, as were genes involved in energy production, muscular trophism and fiber phenotype determination. Importantly, the expression of a gene encoding a component of the nicotinic cholinergic receptor binding site was reduced, suggesting impaired neuromuscular transmission. We argue that these major biological processes could be involved in and/or responsible for the muscle symptoms of CFS.

Published
2009
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39. S-100b protein regulates the activity of skeletal muscle adenylate cyclase in vitro

Author

Fanò, G., Fulle, S., Torre, G.Della, Giambanco, I., Aisa, M.C., Donato, R., and Calissano, P.

Abstract

We have investigated the effect of the b isoform of S-100 proteins on adenylate cyclase activity of rat skeletal muscle. S-100b inhibits the adenylate cyclase activity in the presence of Mg 2+(5.0–50 mM), while it activates the same enzyme in the presence of Ca 2+(0.1–1.0 mM) dose-dependently in both cases. S-100b counteracts the stimulatory effect of NaF on adenylate cyclase in the presence of Mg 2+and the inhibitory effect of RMI 12330 A in the presence of Ca 2+.

Published
1988
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40. Hormonal Receptor Site Alterations in the Etiopathogenesis of Otosclerosis

Author

Maurizi, M., Fanò, G., Paludetti, G., Donti, E., Donti, G., and Ottaviani, F.

Abstract

The authors have studied calcium 45 uptake and cAMP intracellular levels in normal and otosclerotic bone cell cultures after stimulation with calcitonin in the presence or absence of propranolol. The results seem to demonstrate that poststimulatory 45Ca incorporation is slightly different in normal and otosclerotic cell cultures, being slower but longer lasting in the latter. Propranolol administration markedly inhibits 45Ca uptake in normal cells, while in otosclerotic cells a massive intracellular penetration becomes evident after an initial inhibitory phase. Also cAMP intracellular levels behave differently; a marked increase, followed by a rapid decrease, can be detected in normal cells after stimulation with calcitonin, while in otosclerotic cells, the increase is slower and followed by a long lasting reduction. Adding propranolol reduces cAMP levels in normal cells, while it increases levels in otosclerotic cells. The different behavior of calcium metabolism and cAMP levels after stimulation with calcitonin, depending upon the presence or absence of propranolol, seems to indicate an alteration of the transducing mechanism between stimulus, receptor, and cellular effector in otosclerotic cells.

Published
1986
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41. S-100a 0protein stimulates Ca 2+-induced Ca 2+release from isolated sarcoplasmic reticulum vesicles

Author

Fanò, G., Marsili, V., Angelella, P., Aisa, M.C., Giambanco, I., and Donato, R.

Abstract

S-100a 0protein, the αα-isoform of the S-100 family, stimulates Ca 2+-induced Ca 2+release from terminal cisternae isolated from rat skeletal muscle cells. The stimulatory effect of S-100a 0is maximal at ∼5 μM S-100a 0and half maximal at ∼0.1 μM S-100a 0, at 1.8 μM free Ca 2+in the presence of 5 mM Mg 2+plus 0.1 M KCl. The effect of the protein on Ca 2+-induced Ca 2+release is completely inhibted by the calcium release blocker, ruthenium red.

Published
1989
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42. S-100a0protein stimulates the basal (Mg2+-activated) adenylate cyclase activity associated with skeletal muscle membranes

Author

Fanò, G., Angelella, P., Mariggiò, D., Aisa, M.C., Giambanco, I., and Donato, R.

Abstract

S-100a0, protein, the αα isoform of the S-100 family, stimulates basal (Mg2+-activated) adenylate cyclase (AC) activity associated with the sarcolemma, longitudinal tubules and terminal cisternae of rat skeletal muscle cells. The stimulatory effect of S-100a0on AC activity is maximal around 5μM S-100a0and half-maximal around 0.2 μM S-100a0. Also, the stimulatory effect is greatest on the AC activity associated with the terminal cisternae than on the other membrane fractions studied. These data are discussed in relation to the subcellular localization of S-100a0in muscle cells.

Published
1989
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43. S‐100a0protein stimulates Ca2+‐induced Ca2+release from isolated sarcoplasmic reticulum vesicles

Author

Fanò, G., Marsili, V., Angelella, P., Aisa, M.C., Giambanco, I., and Donato, R.

Abstract

S‐100a0protein, the αα‐isoform of the S‐100 family, stimulates Ca2+‐induced Ca2+release from terminal cisternae isolated from rat skeletal muscle cells. The stimulatory effect of S‐100a0is maximal at ∼5 μM S‐100a0and half maximal at ∼0.1 μM S‐100a0, at 1.8 μM free Ca2+in the presence of 5 mM Mg2+plus 0.1 M KCl. The effect of the protein on Ca2+‐induced Ca2+release is completely inhibted by the calcium release blocker, ruthenium red.

Published
1989
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50. Otosclerosis: a new ethiopathogenetic theory

Author

Maurizi, M, Fanò, G, Donti, E, VENTI DONTI, G, Ottaviani, F, and Paludetti, G

Subjects
Settore MED/31 - Otorinolaringoiatria, Settore MED/32 - Audiologia
Published
1984

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