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32 results on '"Fan, Mingliang"'

17. A Novel Combinational Nanodrug Delivery System Induces Synergistic Inhibition of Lung Adenocarcinoma Cells In vitro

Author

Li Jiping and Fan Mingliang

Subjects
Lung, medicine.anatomical_structure, Chemistry, Drug Discovery, medicine, Cancer research, Pharmaceutical Science, Molecular Medicine, Adenocarcinoma, Delivery system, medicine.disease, In vitro
Abstract

Background: The combination of two or more therapeutic drugs is an attractive approach to improve the treatment of experimental tumors. Leveraging nanocarriers for combinational drug delivery can allow control over drug biological fate and promote co-localization in the same area of the body. However, there are certain concerns regarding the biodegradability and potential longterm toxicity arising from these synthetic nanoscale carriers. Objective: Our aim was to develop a combinational nanodrug delivery system formed by selfassembling of amphiphilic drug molecules.minimizing potential toxicities associated with using additional synthetic nanocarriers. Methods: A novel prodrug chlorambucil gemcitabine conjugate was synthesized, this prodrug was used for the encapsulation of an additional hydrophobic anticancer drug paclitaxel, taking the form of combinational nanodrugs. Particle size and zeta potential were evaluated, cytotoxicity assay and apoptosis/cell cycle analysis were also performed to validate the anticancer efficacy of the combinational nanodrugs. Results: The combinational nanodrugs were acquired by means of nanoprecipitation. In A549 lung adenocarcinoma cell line, cellular assays revealed that co-delivery of low dosage paclitaxel with chlorambucil gemcitabine conjugate can act synergistically to inhibit cell growth and induce accumulation of cells in the G2/M phase with a concomitant decrease in G0/G1 compartment. Conclusion: Chlorambucil gemcitabine conjugate and paclitaxel can co-assemble into composite nanoparticles by a nanoprecipitation process and the resulting combinational nanodrugs showed a synergistic anticancer effect. This synthetic nanocarrier-free approach might broaden the nanodrug concept and have potential in cancer therapy.

Published
2021

19. Two-pot Oxidative Preparation of Dicarboxylic Acid Containing Cellulose for the Removal of Beryllium (Be2+) from Aqueous Solution

Author

Cumming Paul, Li Jiping, M. Siddeeg Saifeldin, Katoch Ashish, Alam Intyaz, Fan Mingliang, A. Tahoon Mohamed, Singh Manjinder, Tolga Özdemir Vedat, Yayayürük Onur, Mert Tuğaç Himmet, Aggarwal Geeta, Arar Özgür, Kumar Kashyap Pankaj, Kaur Malkiet, B. Rebah Faouzi, Shabbir Muhamad, Kumar Sushil, Verma Sonia, Mangipudi Parthasarathi, Henriksen Gjermund, Yadav Arvind, Arora Monika, Marton Janos, Baranwal Somesh, Bauer Beate, S. Alsaiari Norah, Erdem Yayayürük Aslı, Arora Dhingra Gitika, Kumar Pradeep, Nagpal Manju, Ratan Ram, and Singh Pathania Akashdeep

Subjects
chemistry.chemical_classification, Behavior, Aqueous solution, chemistry.chemical_element, Water, Oxidative phosphorylation, water treatment, Analytical Chemistry, chemistry.chemical_compound, Dicarboxylic acid, chemistry, biopolymer, regeneration, cellulose diacetate, Sorption, ion-exchange, Beryllium, Cellulose, Biosorbents, Nuclear chemistry
Abstract

Background: Cellulose is one of the most abundant, non-toxic, and renewable natural biopolymers. The presence of hydroxyl groups in cellulose leads to further modification of it. Preparation and modification of cellulose-based sorbents and their applications on water treatment gained traction in recent years. Objective: A low-cost and eco-friendly biosorbent was designed and fabricated by introducing the acetate functional groups into cellulose for removing Beryllium (Be2+) from an aqueous solution. The sorption of Be2+ on acetate containing cellulose was evaluated for varying sorbent doses and initial solution pH values. Methods: The sorbent was prepared by a two-step oxidation process. In the initial step, cellulose reacted with NaIO4 and aldehyde groups were introduced to the cellulose. In the second step, newly obtained aldehyde groups were oxidized to create acetate groups. Results: The kinetics of the sorption process showed that Be2+ uptake reached equilibrium in 3 minutes. The sorption isotherm was well fitted in the Langmuir model, and the maximum sorption capacity was 4.54 mg/g. Moreover, the thermodynamic studies demonstrated that Be2+ sorption was spontaneous and exothermic. Furthermore, the prepared sorbent can be regenerated by using 0.1 M HCl or H2SO4 solutions. Conclusion: It is concluded that the removal of Be2+ is pH-dependent and it is favorable at high solution pH. The kinetics of the prepared sorbent were rapid and equilibrium attained in 3 minutes. The prepared sorbent can be regenerated with 0.1 M acid solution with > 99% efficiency., Ege University Scientific Re-search Projects Coordination Unit [FLP-2018-20310], This study is supported by Ege University Scientific Re-search Projects Coordination Unit (Project Number: FLP-2018-20310) .

Published
2022

28. Epidermal growth factor receptor-targeted peptide conjugated phospholipid micelles for doxorubicin delivery.

Author

Fan, Mingliang, Liang, Xiaofei, Yang, Danbo, Pan, Xiaorong, Li, Zonghai, Wang, Hongyang, and Shi, Bizhi

Subjects
*EPIDERMAL growth factor receptors, *DOXORUBICIN, *PHOSPHOLIPIDS, *MICELLES, *TARGETED drug delivery
Abstract

Specific targeting of tumor cells to achieve higher drug levels in tumor tissue and to overcome side effects is the major goal in cancer chemotherapy. In this study, we used a tumor targeting peptide, GE11, to conjugate onto the surface of doxorubicin encapsulated phospholipid micelles. The GE11 peptide triggered specific binding to epidermal growth factor receptor (EGFR), leading to enhanced cellular uptake and cytotoxicity in vitro and highly accumulation in the tumors in vivo. The results indicated that GE11 conjugated phospholipid micelles should have potential applications in cancer therapy. [ABSTRACT FROM AUTHOR]

Published
2016
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31. [Influence of Lactobacillus reuteri SL001 on intestinal microbiota in AD model mice and C57BL/6 mice].

Author

Liu M, Hu R, Guo Y, Sun W, Li J, Fan M, Wang Y, Du H, Tang Z, and Chai C

Subjects
Animals, Lactobacillus, Male, Mice, Mice, Inbred C57BL, RNA, Ribosomal, 16S genetics, Gastrointestinal Microbiome, Limosilactobacillus reuteri
Abstract

We studied the influence of Lactobacillus reuteri SL001 (L. reuteri SL001) on the gut microbial community in Alzheimer's disease model mice (APP/PS1 double transgenic mice, ADmice) and wild type (C57BL/6) mice. The AD model mice and wild type mice were divided into test and control groups (4 in total), with 5 mice each and only male mice. The test group was fed with 0.2 mL suspension of L. reuteri SL001 at 5×10¹¹ CFU/mL. The control group received the same amount of sterile PBS daily for 45 days. Fecal samples were collected to compare and analyze the community structure and diversity of microbiota through high-throughput sequencing of the V3-V4 region of 16S rRNA gene. By sequence alignment and classification, the intestinal microbial OTUs of the 4 groups including 19 phyla, 40 classes, 64 orders, 104 families and 175 genera. The α diversity of AD model mice was greater than that of wild type mice, but the difference between the two was small. After adding L. reuteri SL001, the α diversity of both mice increased, and the increase in AD model mice was smaller. At the phyla level, the dominant phyla of the four groups of mice were Bacteroidetes, Firmicutes and Proteobacteria. The abundance of Bacteroides phylum in AD model mice was lower than that of wild type, and the abundance of chlamydomonas was higher than that of wild type. Feeding L. reuteri SL001 reduced the proportion of Firmicutes/Bacteroidetes (F/B) in mice. At the order level, the relative abundance of Bacteroidales, Lactobacillales, Bacillales and Bifidobacteriales in AD model mice was lower than that of wild type mice. At the genus level, the abundant genera were Allobaculum, Lachnospiraceae_NK4A136_group, Bacteroides and Lactobacillus. The relative abundance of Bacteroides, Lactobacillus, Bifidobacterium and Alloprevotella in AD model mice was lower than that in wild type mice. Adding L. reuteri SL001 increased the abundance of these genera and Bacteroides, Lactobacillus, Bacillus and Bifidobacteria in AD model mice. The relative abundance of Butyrivibrio in AD model mice was also lower than that in wild type, but after the feeding of L. reuteri SL001, the relative abundance was reduced in both mice. The dominant strains of wild-type mice were Lactobacillus, and no dominant flora was found in AD model mice. The results in this article provide valuable data for revealing the difference in intestinal microbial diversity between AD model mice and C57BL/6 mice, and feeding L. reuteri SL001 play positive roles in adjusting the intestinal bacterial community structure of AD model mice.

Published
2020
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