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Abstract: A broadband and compact coplanar waveguide (CPW) coupled‐fed metasurface (MS)‐based antenna for C‐band synthetic aperture radar (SAR) imaging application is proposed in this article, which is consisted of 16 uniform periodic square patches performed as radiators. The CPW feeding structure gives two following functions: (1) It excites an aperture coupling slot structure underneath the center of MS patch array. (2) It acts as a ground plane for the metasurface patch units. Different slots were investigated and eventually an hourglass‐shaped slot is applied to enhance bandwidth for imaging applications. A prototype with a dimension of 60 × 60 × 1.524 mm3 (1.1λ0 × 1.1λ0 × 0.03λ0) operating at the center frequency 5.5 GHz (f0) has been fabricated and measured to verify the design principle. This antenna has a measured impedance bandwidth of 12.4% from 5.14 to 5.82 GHz, a peak gain of 9.2 dBi and averaged gain of 7.2 dBi at broadside radiation. Microwave imaging experiments using the proposed antenna have been carried out and a good performance is achieved. [ABSTRACT FROM AUTHOR]

Objective: To investigate low density lipoprotein receptor (LDLR) function and gene mutation in Chinese patients with familial hypercholesterolemia(FH)., Methods: Lymphocytes were isolated from 10 ml anticoagulated peripheral blood of the patients, then a flow-cytometric method (FCM) with 1,1'-dioctadecyl-3,3,3', 3-tetramethylindocarbocyanine perchlorate labelled low density lipoproetin (DiI-LDL) was used to identify the function of LDLR on the surface of lymphocytes. Genomic DNA was isolated from whole blood of FH patients and analyzed by PCR-single strand conformation polymorphism (SSCP) and nucleotide sequencing methods., Results: Defects of binding and uptaking of LDLR were identified by FCM in 2 FH patients in one family, and their parents were examined in the present study. Then they were analyzed genetically. The detected mutation was a deletion of A, which caused a frame shift in codon 297 of exon 6 and introduced a beforehand stop codon in codon 369., Conclusion: A novel mutation of LDL receptor gene was detected by the combination of FCM and PCR-SSCP methods.

Objective: To analyse the LDL receptor (LDLR) function and gene mutation in a familial hypercholesterolemia (FH) patient and illustrate the effects of gene mutation type on LDL receptor function., Methods: The pedigree of a FH proband was set up according to the serum lipid analysis and clinical presentations. The LDLR functions of cultured fibroblasts were investigated by radiolabelled ligand method. PCR-SSCP and DNA sequencing were performed on the genomic DNA isolated from whole blood, Results: 11 heterozygotes and 1 homozygote of FH were confirmed by pedigree analysis. The binding of LDL by LDLR of the proband was nearly normal while the uptake and degradation of LDL were only 3.6% and 1.7% as compared with controls. A frameshift mutation resulted from a G insert in codon 599 and a null mutation caused by CCA-->CCG base shift in codon 842 were found in exon 17., Conclusion: A novel mutation of LDLR gene was reported. This mutation may severely affect the function of LDLR.