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The purpose of this study was to develop a total nutrition emulsion suitable for post-gastrectomy patients. The effects of different protein types, maltodextrins with different dextrose equivalent (DE) values, and different oils on the stability of the emulsion were investigated by measuring the particle size and sedimentation rate, and the effects on blood glucose and body mass in post-gastrectomy mice were also assessed. Furthermore, the number of homogenization cycles, homogenization pressure and pH were optimized for the processing of the emulsion. The results showed that good stability was observed in the emulsion made with whey protein as a protein source, maltodextrin with a DE value of 12, and a mixture of peanut oil and caprylic triglyceride or capric triglyceride at a ratio of 2:1, which was most favorable for restoring blood glucose and body mass in post-gastrectomy mice. The emulsion processed by 7 homogenization cycles at 70 kPa and pH 7.0 had the smallest particle size, the lowest sedimentation rate and the best stability. The findings obtained in this study provide theoretical guidance for the research and development of special emulsion-type foods for post-gastrectomy patients.

The liquid lead-bismuth cooled fast reactor has been in a single-phase, low-pressure, and high-temperature state for a long time during operation. Considering the requirement of calculation efficiency for long-term transient accident calculation, based on a homogeneous hydrodynamic model, one-dimensional heat conduction model, coolant flow and heat transfer model, neutron kinetics model, coolant and material properties model, this study used the fully implicit difference scheme algorithm of the convection-diffusion term to solve the basic conservation equation, to develop the transient analysis program NUSOL-LMR 2.0 for the lead-bismuth fast reactor system. The steady-state and typical design basis accidents (including reactivity introduction, loss of flow caused by main pump idling, excessive cooling, and plant power outage accidents) for the ABR have been analyzed. The results are compared with the international system analysis software ATHENA. The results indicate that the developed program can stably, accurately, and efficiently predict the transient accident response and safety characteristics of the lead-bismuth fast reactor system.

This study aimed to compare the postoperative analgesic efficacy and motor recovery of a novel lumbar plexus block (LPB) with that of a femoral nerve block (FNB) after total knee arthroplasty (TKA). Forty patients who underwent TKA were randomised equally into an lumbar plexus and sciatic nerve (LS) group (receiving novel LPB) and an femoral and sciatic nerves (FS) group (receiving FNB). The assessed variables were the onset time of pain, time to the first analgesic request, pain scores, motor block at 6, 12, and 24 h after TKA, and the number of patients receiving successful blockade for each branch of the lumbar plexus. In the LS group, the femoral, lateral femoral cutaneous, genitofemoral, iliohypogastric, ilioinguinal, and obturator nerves were blocked in 18, 20, 16, 18, 15, and 19 patients. Compared to the FS group, the LS group had a significantly shorter onset time of pain and time to the first analgesic request, a significantly larger total postoperative dose of sufentanil, significantly higher numeric rating scale scores for both rest and dynamic pain at 6, 12, and 24 h, and faster motor recovery. Novel ultrasound-guided LPB has a high blocking success rate and provides inferior postoperative analgesia, but faster motor recovery after TKA than FNB.

In pancreatic cancer, KRAS G12D can trigger pancreatic cancer initiation and development. Rapid tumor growth is often accompanied by excess intracellular reactive oxygen species (ROS) production, which is unfavorable to tumor. However, the regulation of intracellular ROS levels in KRAS mutant pancreatic cancer remains unclear. In this study, we establish BxPC3 stable cell strains expressing KRAS wild type (WT) and G12D mutation and find unchanged ROS levels despite higher glycolysis and proliferation viability in KRAS mutant cells than KRAS WT cells. The key hydrogen sulfide (H 2S)-generating enzyme cystathionine-γ-lyase (CSE) is upregulated in KRAS mutant BxPC3 cells, and its knockdown significantly increases intracellular ROS levels and decreases cell glycolysis and proliferation. Nuclear factor erythroid 2-related factor 2 (Nrf2) is activated by KRAS mutation to promote CSE transcription. An Nrf2 binding site (‒47/‒39 bp) in the CSE promoter is verified. CSE overexpression and the addition of NaHS after Nrf2 knockdown or inhibition by brusatol decreases ROS levels and rescues cell proliferation. Our study reveals the regulatory mechanism of intracellular ROS levels in KRAS mutant pancreatic cancer cells, which provides a potential target for pancreatic cancer therapy.

Abstract Fresh mulberry leaf vegetable is nutritive and becoming popular. However, available preservation technologies are deficient. In present work, the effects of two kinds of modified atmosphere packaging on postharvest quality of fresh mulberry leaf vegetable stored at 4 °C were evaluated. The respiration rate of samples in the modified polyethylene packages (MP20) was 12.88–22.65% lower than that in normal polyethylene packaging (CK). The content of total soluble solids, soluble protein, and total polyphenol in MP20 was less changed than that in CK, and the vitamin C retention was higher as well. Moreover, the lignin content in MP20 was lower than that in CK during storage (19.79% vs 13.38% at day 8), and that was significantly positively related to the polyphenol oxidase and peroxidase activities inhibition. Taken together, a packaging with moderate gas permeability (MP20) is suitable for nutrition maintenance and lignification inhibition of fresh mulberry leaf vegetable during cold storage.