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Executive functioning is a cognitive process that coordinates and organizes other mental processes to adapt to the demands of the environment, with the inhibition prepotent responses being a fundamental skill of this process. Inhibition involves interpreting signals and monitoring conflicts, activating the anterior cingulate cortex for greater cognitive control. The attentional control theory suggests that inhibition and attentional control share brain substrates, and their interruption affects the capacity for inhibition and switching. In a task where preponderant responses to certain stimuli must be inhibited, such as the Go/No-Go task, cultural familiarity could influence performance, since exposure to culturalized elements can become preponderant responses. However, this effect has not been fully explored. To explore this phenomenon, an observational study was carried out with 15 normotypical participants subjected to the Go/No-Go task in two versions: the traditional task and a variant with novel stimuli. The correct answers, the reaction time, the commissions, and omissions of both versions were analyzed. The results suggest that there is a large effect size (d = 1.16, p = < 0.001) reaction time differential between the two versions in favor of the traditional version, which responded more quickly. Additionally, the concordance and correspondence measures obtained a low similarity (-0.06 [-0.36 | 0.25]) between the reaction times of the traditional and alternative tasks. It is concluded that the data suggest the influence of a differential effect when using a culturally learned stimulus and one that is not, although this effect is concentrated in response times. It is suggested to pay attention to this factor as an active element of the Go/NoGo task in future studies. [ABSTRACT FROM AUTHOR]