Search

Your search keyword '"Familari Mary"' showing total 154 results
Start Over Author "Familari Mary"
154 results on '"Familari Mary"'

2. Comparative analysis of the ATRX promoter and 5' regulatory region reveals conserved regulatory elements which are linked to roles in neurodevelopment, alpha-globin regulation and testicular function

Author

Argentaro Anthony, Frankenberg Stephen, Tang Paisu, Graves Jennifer M, and Familari Mary

Subjects
Medicine, Biology (General), QH301-705.5, Science (General), Q1-390
Abstract

Abstract Background ATRX is a tightly-regulated multifunctional protein with crucial roles in mammalian development. Mutations in the ATRX gene cause ATR-X syndrome, an X-linked recessive developmental disorder resulting in severe mental retardation and mild alpha-thalassemia with facial, skeletal and genital abnormalities. Although ubiquitously expressed the clinical features of the syndrome indicate that ATRX is not likely to be a global regulator of gene expression but involved in regulating specific target genes. The regulation of ATRX expression is not well understood and this is reflected by the current lack of identified upstream regulators. The availability of genomic data from a range of species and the very highly conserved 5' regulatory regions of the ATRX gene has allowed us to investigate putative transcription factor binding sites (TFBSs) in evolutionarily conserved regions of the mammalian ATRX promoter. Results We identified 12 highly conserved TFBSs of key gene regulators involved in biologically relevant processes such as neural and testis development and alpha-globin regulation. Conclusions Our results reveal potentially important regulatory elements in the ATRX gene which may lead to the identification of upstream regulators of ATRX and aid in the understanding of the molecular mechanisms that underlie ATR-X syndrome.

Published
2011
Full Text
View/download PDF

6. When Majority Doesn't Rule: The Use of Discrimination Indices to Improve the Quality of MCQs

Author

Chiavaroli, Neville and Familari, Mary

Abstract

This paper outlines the use of item analysis to assist examiners in evaluating the quality and validity of their MCQ exam questions. The generation of item analysis, particularly discrimination index, has long been established practice in professional testing and credentialing organisations and some disciplines in tertiary education, but its use appears to be inconsistent among Bioscience departments. We argue that generating some form of discrimination index is an essential part of the validation of exams, in particular to help identify errors in scoring, to identify potentially flawed questions, or to confirm the validity of challenging questions. We demonstrate each of these uses through examples drawn from first year Biology exams, with interpretations of the questions in the light of post-examination item analysis.

Published
2011
Full Text
View/download PDF

8. Regulation of anterior pituitary adrenocorticotropin secretion : in vitro studies

Author

Familari., Mary

Subjects
ComputingMilieux_COMPUTERSANDEDUCATION, ComputerApplications_COMPUTERSINOTHERSYSTEMS, Uncategorized
Abstract

This thesis was scanned from the print manuscript for digital preservation and is copyright the author. Researchers can access this thesis by asking their local university, institution or public library to make a request on their behalf. Monash staff and postgraduate students can use the link in the References field.

Published
2021
Full Text
View/download PDF

12. Short-chain fatty acids inhibit intestinal trefoil factor gene expression in colon cancer cells

Author

Tran, Chau P., Familari, Mary, Parker, Lorraine M., Whitehead, Robert H., and Giraud, Andrew S.

Subjects
Fatty acids -- Physiological aspects, Gene expression -- Physiological aspects, Colorectal cancer -- Genetic aspects, Cells -- Physiological aspects, Biological sciences
Abstract

A study was conducted to examine the inhibitory effects of short-chain fatty acids on intestinal trefoil factor (ITF) gene expression in colon cancer cells. The colon cancer cell lines were analyzed for treoil immunoreactivity before being cultured to 80% confluency. The cells were then treated with or without 2 mM butyrate for 24 hours. Results indicated that butyrate inhibits ITF expression in colon cancer cells.

Published
1998

15. Preeclampsia is Associated with Sex-Specific Transcriptional and Proteomic Changes in Fetal Erythroid Cells

Author

Masoumi, Zahra, primary, Maes, Gregory E., additional, Herten, Koen, additional, Cortés-Calabuig, Álvaro, additional, Alattar, Abdul Ghani, additional, Hanson, Eva, additional, Erlandsson, Lena, additional, Mezey, Eva, additional, Magnusson, Mattias, additional, Vermeesch, Joris R, additional, Familari, Mary, additional, and Hansson, Stefan R, additional

Published
2019
Full Text
View/download PDF

23. Resistance to apoptosis is a mechanism of adaptation of rat stomach to aspirin

Author

ALDERMAN, BARBARA M., COOK, GREGORY A., FAMILARI, MARY, YEOMANS, NEVILLE D., and GIRAUD, ANDREW S.

Subjects
Cell death -- Research, Rats -- Physiological aspects, Aspirin -- Adverse and side effects, Gastric mucosa -- Physiological aspects, Heat shock proteins -- Research, Biological sciences
Abstract

Alderman, Barbara M., Gregory A. Cook, Mary Familari, Neville D. Yeomans, and Andrew S. Giraud. Resistance to apoptosis is a mechanism of adaptation of rat stomach to aspirin. Am J Physiol Gastrointest Liver Physiol 278: G839-G846, 2000.--Adaptation of the gastric mucosa to nonsteroidal anti-inflammatory drug-induced injury is a well-documented phenomenon, but the mechanisms are not known. We investigated whether changes in stress protein expression and apoptosis play roles in adaptation of rat stomach to aspirin. RT-PCR and Western blotting techniques were used to analyze mRNA and protein expression of HSP72 and HSP90 and cleavage of caspase 3 protein. Apoptosis was detected by the TUNEL method and quantified. HSP72 mRNA and protein expression was unchanged in adapted mucosa, whereas HSP90 mRNA and protein levels decreased. Caspase 3 protein was activated, and the number of apoptotic cells increased in mucosa after one aspirin dose. However, in adapted mucosa after aspirin, activated caspase 3 and the number of apoptotic cells had returned to basal levels. Induction of the stress response was found not to be a mechanism of mucosal adaptation against multiple doses of aspirin. Our results lead us to propose instead that resistance to aspirin-induced apoptosis plays a role in the protective phenomenon of adaptation. stress proteins; caspase 3; nonsteroidal anti-inflammatory drugs; gastric mucosa

Published
2000

28. Syncytiotrophoblast Vesicles Show Altered micro-RNA and Haemoglobin Content after Ex-vivo Perfusion of Placentas with Haemoglobin to Mimic Preeclampsia.

Author

Cronqvist, Tina, Saljé, Karen, Familari, Mary, Guller, Seth, Schneider, Henning, Gardiner, Chris, Sargent, Ian L, Redman, Christopher W, Mörgelin, Matthias, Åkerström, Bo, Gram, Magnus, Hansson, Stefan, Cronqvist, Tina, Saljé, Karen, Familari, Mary, Guller, Seth, Schneider, Henning, Gardiner, Chris, Sargent, Ian L, Redman, Christopher W, Mörgelin, Matthias, Åkerström, Bo, Gram, Magnus, and Hansson, Stefan

Abstract

Cell-free foetal haemoglobin (HbF) has been shown to play a role in the pathology of preeclampsia (PE). In the present study, we aimed to further characterize the harmful effects of extracellular free haemoglobin (Hb) on the placenta. In particular, we investigated whether cell-free Hb affects the release of placental syncytiotrophoblast vesicles (STBMs) and their micro-RNA content.

Published
2014

29. Characteristics of the Endoderm: Embryonic and Extraembryonic in Mouse

Author

Familari, Mary

Subjects
Article Subject, embryonic structures
Abstract

In mouse, four endodermal lineages are generated during the period from the late blastocyst to the end of gastrulation. The characteristics of each lineage and the proposed genetic cascades involved in their formation are reviewed. In addition, a list of the current markers used to identify these lineages in vivo and in vitro is presented.

Published
2006
Full Text
View/download PDF

33. Syncytiotrophoblast Vesicles Show Altered micro-RNA and Haemoglobin Content after Ex-vivo Perfusion of Placentas with Haemoglobin to Mimic Preeclampsia

Author

Cronqvist, Tina, primary, Saljé, Karen, additional, Familari, Mary, additional, Guller, Seth, additional, Schneider, Henning, additional, Gardiner, Chris, additional, Sargent, Ian L., additional, Redman, Christopher W., additional, Mörgelin, Matthias, additional, Åkerström, Bo, additional, Gram, Magnus, additional, and Hansson, Stefan R., additional

Published
2014
Full Text
View/download PDF

34. Stem Cells as In Vitro Models of Disease

Author

Dottori, Mirella, Familari, Mary, Hansson, Stefan, Hasegawa, Kouichi, Dottori, Mirella, Familari, Mary, Hansson, Stefan, and Hasegawa, Kouichi

Published
2012

35. Comparative analysis of the ATRX promoter and 5' regulatory region reveals conserved regulatory elements which are linked to roles in neurodevelopment, alpha-globin regulation and testicular function

Author

Tang, Paisu, Frankenberg, Stephen, Argentaro, Anthony, Graves, Jennifer M, Familari, Mary, Tang, Paisu, Frankenberg, Stephen, Argentaro, Anthony, Graves, Jennifer M, and Familari, Mary

Abstract

BACKGROUND ATRX is a tightly-regulated multifunctional protein with crucial roles in mammalian development. Mutations in the ATRX gene cause ATR-X syndrome, an X-linked recessive developmental disorder resulting in severe mental retardation and mild alpha-thalassemia with facial, skeletal and genital abnormalities. Although ubiquitously expressed the clinical features of the syndrome indicate that ATRX is not likely to be a global regulator of gene expression but involved in regulating specific target genes. The regulation of ATRX expression is not well understood and this is reflected by the current lack of identified upstream regulators. The availability of genomic data from a range of species and the very highly conserved 5' regulatory regions of the ATRX gene has allowed us to investigate putative transcription factor binding sites (TFBSs) in evolutionarily conserved regions of the mammalian ATRX promoter. RESULTS We identified 12 highly conserved TFBSs of key gene regulators involved in biologically relevant processes such as neural and testis development and alpha-globin regulation. CONCLUSIONS Our results reveal potentially important regulatory elements in the ATRX gene which may lead to the identification of upstream regulators of ATRX and aid in the understanding of the molecular mechanisms that underlie ATR-X syndrome.

Published
2011

Catalog

Books, media, physical & digital resources
See catalog results