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Freitas A.Q., Florião M.M., Lourenço E.C., Moya-Borja G.E., Couri M.S. & Famadas K.M. [Dipterofauna with enphasis in Muscidae associated to cattle manure in a tropical organic dairy system from Brazil.] Dipterofauna com ênfase em Muscidae associada às massas fecais de bovinos em sistema orgânico de produção de leite, na região tropical, Brasil. Revista Brasileira de Medicina Veterinária, 38(supl. 3):218 - 228, 2016.. Programa de Pós-Graduação em Ciências Veterinárias, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro. Universidade Federal Rural do Rio de Janeiro. BR 465 km 7. Campus Seropédica, Rio de Janeiro, RJ 23.897-970, Brasil. E-mail: alineqf@hotmail.com. Dipterans are important members of the decomposition fauna of cattle dung and they influence the incidence of parasitic diseases in the herd. Thus, the present study aimed to make an inventory of the members of the dipterofauna associated with manure in an organic system of dairy cattle in Seropédica, State of Rio de Janeiro. Four litres of manure were collected dairy during one month in each season and immediately taken to the laboratory where we extracted the pupas of the dipterans by flotation method, and then we individualized each pupa in an Eppendorf for the emergence of the adults. After that, we identified the dipterans and tabulated the results. The Diptera families reported belonged to the families Muscidae, Sepsidae, Sarcophagidae, Stradiomyidae and Tachinidae. About the muscids, we collected the following species: Gymnodia normata, Gymnodia quadristigma, Musca domestica and Biopyrellia bipuncta., Dípteros são importantes membros da coprofauna decompositora do esterco bovino e influenciam na incidência de parasitoses nos rebanhos bovinos. Neste sentido, o estudo teve como objetivo inventariar e quantificar os integrantes da dipterofauna associada a massas fecais de bovinos leiteiros orgânicos em Seropédica, Estado do -Rio de Janeiro. Massas fecais bovinas foram coletadas em um sistema de produção de leite orgânico, e as pupas foram extraídas através do método de flotação. Após a emergência, os dípteros adultos foram identificados e os resultados tabulados. As famílias de dípteros coletados foram Muscidae, Sepsidae, Sarcophagidae, Stratiomyidae e Tachinidae. Quanto aos muscideos, foram coletados Gymnodia normata, Gymnodia quadristigma, Musca domestica e Biopyrellia bipuncta.

Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2021-06-23T16:08:46Z No. of bitstreams: 1 2018 - Guilherme Pinheiro Furusawa.pdf: 2092982 bytes, checksum: 6edea2ed8718ae42a2d4954357f3402b (MD5) Made available in DSpace on 2021-06-23T16:08:46Z (GMT). No. of bitstreams: 1 2018 - Guilherme Pinheiro Furusawa.pdf: 2092982 bytes, checksum: 6edea2ed8718ae42a2d4954357f3402b (MD5) Previous issue date: 2018-02-26 Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq With the increasing advance of the antropobiocenoses towards the wild areas, resulting in decrease and fragmentation of native forests, it notes as a result of this approach, greater interaction between various agencies before separated. Among the new relationships that are established, there are several commensals and parasites, which eventually adapt to this new reality, approaching the anthropized environments, attracted by the accumulation of biomass or associated with domestic animals. Of the domestic animals commonly found with man, the domestic dog, Canis lupus familiaris (Linnaeus, 1758) stands out, being able to serve as host of several wild parasites, with an emphasis on ticks (Acari: Ixodidae). The study on screen investigated the distribution of ticks associated to the semi-domiciled or errant domestic dog, found in communities located in areas of the Ecological Corridor of Serra do Mar, in three nuclei of the State Park of Serra do Mar, SP, S?o Sebasti?o, PESN -NSS, Picinguaba, PESN-NP and Cunha, PESN-NC, as well as an area of the Serra da Bocaina National Park in Paraty, RJ. In all the studied areas 608 ticks were collected. Of these, 585 were collected in areas under the influence of Submontane Dense Ombrophylous Forest and Dense Lowland Ombrophilous Forest, with altitudes up to 600 meters, and 23 collected in an area under the influence of the Dense Ombrophylous Forest Altomontana and Ombrophilous Dense Montana Forest, with altitudes between 600 and 1,500m. All ticks collected were from the Ixodidae family, from the genera Amblyomma and Rhipicephalus. In the collections of ticks carried out in the areas under the influence of the Submontane Dense Ombrophylous Forest and the Dense Ombrophylous Lowland Forest, 19 free - living ticks were collected, all belonging to the genus Amblyomma. Of these, six A. ovale, nine A. naponense collected in the PESN-NP and two A. ovale and two nymphs of A. sculptum collected in the PESN-NSS. A total of 23 animals were examined. Of these, 129 (56,08 %) presented infestation by ticks, from which 589 specimens were collected, 352 of which were collected in the PESM-NP area, 126 collected in the non-PESM area -NSS and 88 ticks collected in Paraty, in the PNSB area. The species collected were: 332 (56.36%) A. ovale (Koch, 1844), 230 (39.09%) R. sanguineus sensu lato (Latreille, 1806), 17 (2.88%) A. aureolatum (Pallas, 1772), and 05 (0.84%) A. sculptum (Berlese, 1888), plus 05 larvae that were not identified. It was considered the distribution of these species in relation to the proximity of forest areas, where, A. ovale was the species that occurred the most in these areas up to the altitude of 600m. R. sanguineus was present in all areas of collection, with greater occurrence in areas farthest from forest areas Com o crescente avan?o das antropobiocenoses em dire??o ?s ?reas silvestres, que resulta na diminui??o e fragmenta??o das florestas nativas, constata-se, como resultado desta aproxima??o, uma maior intera??o entre diversos organismos antes separados. Dentre as novas rela??es que se estabelecem, destacam-se a de diversos comensais e parasitas, que eventualmente se adaptam a esta nova realidade, aproximando-se dos ambientes antropizados, atra?dos pelo ac?mulo de biomassa ou associados aos animais dom?sticos. Dos animais dom?sticos comumente encontrados com o homem, o c?o dom?stico, Canis lupus familiaris (Linnaeus, 1758) se destaca, podendo servir como hospedeiro de diversos parasitos silvestres, com ?nfase aos carrapatos (Acari: Ixodidae). O trabalho em tela investigou a distribui??o de carrapatos associados ao c?o dom?stico semidomiciliado ou errante, encontrados em comunidades inseridas em ?reas do Corredor Ecol?gico da Serra do Mar, em tr?s n?cleos do Parque Estadual da Serra do Mar, SP, n?cleos: S?o Sebasti?o, PESN- NSS, Picinguaba, PESN-NP e Cunha, PESN-NC, al?m de uma ?rea do Parque Nacional da Serra da Bocaina, em Paraty, RJ. Em todas as ?reas estudadas foram coletados 608 carrapatos, destes, 585 foram coletados em ?reas sob influ?ncia de Floresta Ombr?fila Densa Submontana e Floresta Ombr?fila Densa de Terras Baixas, com altitudes de at? 600 metros, e 23 coletados em ?rea sob influ?ncia da Floresta Ombr?fila Densa Altomontana e Floresta Ombr?fila Densa Montana, com altitudes entre 600 e 1.500m. Todos os carrapatos coletados foram da fam?lia Ixodidae, dos g?neros Amblyomma e Rhipicephalus. Foram examinados 230 animais, dentre estes, 129 (56,08%) apresentavam infesta??o por carrapatos, de onde foram coletados 589 exemplares, sendo 352 coletados em ?rea do PESM-NP, 126 coletados em ?rea do no PESM-NSS e 88 carrapatos coletados em Paraty, em ?rea do PNSB e 23 em Cunha. As esp?cies coletadas foram: 332 (56,36 %) A. ovale (Koch, 1844), 230 (39,04%) R. sanguineus sensu lato (Latreille, 1806), 17 (2,88%) A. aureolatum (Pallas, 1772), 05 (0,84%) A. sculptum (Berlese, 1888), al?m de 05 larvas que n?o foram identificadas. Considerou-se a distribui??o destas esp?cies com rela??o ? proximidade com as ?reas florestais onde, A. ovale foi a esp?cie que mais ocorreu nestas ?reas at? a altitude dos 600m. R. sanguineus esteve presente em todas as ?reas de coleta, com maior ocorr?ncia nas ?reas mais urbanizadas. Nas coletas de carrapatos em vida livre, realizadas nas ?reas sob influ?ncia da Floresta Ombr?fila Densa Submontana e Floresta Ombr?fila Densa de Terras Baixas, foram coletados 19 carrapatos, todos pertencentes ao g?nero Amblyomma. Destes, seis A. ovale, nove A. naponense coletados no PESN-NP e dois A. ovale e duas ninfas de A. sculptum coletadas no PESN-NSS.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2019-09-03T18:26:11Z No. of bitstreams: 1 2015 - Elizabete Captivo Louren?o.pdf: 2878428 bytes, checksum: d0d2db6930e1830b67399375768dded6 (MD5) Made available in DSpace on 2019-09-03T18:26:11Z (GMT). No. of bitstreams: 1 2015 - Elizabete Captivo Louren?o.pdf: 2878428 bytes, checksum: d0d2db6930e1830b67399375768dded6 (MD5) Previous issue date: 2015-02-24 CNPq Few is known of the ecological aspects of most species of ectoparasites of bats, their relationship with the host and how environmental factors and/or the host itself act on the variation of their populations. Bats are home to a wide variety of ectoparasites, including the Streblidae flies and Spinturnicidae mites. These ectoparasites are haematophagous, exclusive of bats and maintains a strong relationship specificity and coevolution with phyllostomid bats, which become them good taxa to infer about possible variations between areas. Whereas areas with different landscape characteristics to determine differences in parasitism of Streblidae and Spinturnicidae in phyllostomid bats was realized a study of the compositions of the assemblage and components of populations and components of communities of three areas of riparian forest inserted on Atlantic Forest, Nova Igua?u city, Tingu? region, State of Rio de Janeiro, Brazil. Two areas in the buffer zone of Biological Tingu? Reserve, an agricultural area, Takume, and a reforestation area, Canavarro, and an area in within the Biological Reserve of Tingu?, Rebio, considered the most preserved area. The sampling effort was similar in all three areas, with monthly sampling, a total of 12 nights in each area, between May 2011 and April 2012.It was registerd 22 species of bats phyllostomid, 24 species of bat flies Streblidae and five Spinturnicidae mites total of the three areas. The Rebio had the highest richness of bats and ectoparasites, while Canavarro had the greatest abundance of individuals and parasitological rates, prevalence, intensity and mean abundance of infestation. The greatest richness of Rebio was attributed to the greater degree of preservation of the area that allowed highest richness of bats and thus their associated ectoparasites. The highest abundance, as well as the highest parasitological indices on Canavarro was attributed to the high abundance of two species of bats, Artibeus lituratus and Sturnira lilium. Both species are commonly found infested by specimens of Streblidae and Spinturnicidae. The data reinforce the idea of high specificity of these ectoparasites with their hosts. Since the parasitism of the areas followed the variations of bats assembleges, showing no variations that could be associated with variables other than their preferred hosts. Pouco se conhece dos aspectos ecol?gicos da maioria das esp?cies de ectoparasitos de morcegos, da rela??o destes com o hospedeiro e como os fatores ambientais e/ou do pr?prio hospedeiro agem na varia??o de suas popula??es. Morcegos albergam uma grande variedade de ectoparasitos, dentre eles, as moscas Streblidae e os ?caros Spinturnicidae. Esses ectoparasitos s?o hemat?fagos, exclusivos de morcegos e mantem uma forte rela??o de especificidade e coevolu??o com morcegos Phyllostomidae, o que os torna bons taxa para inferir a respeito de poss?veis varia??es entre ?reas. Considerando que ?reas com diferentes caracter?sticas de paisagem possam determinar diferen?as no ectoparasitismo de Streblidae e Spinturnicidae nos morcegos Phyllostomidae foi realizado um estudo das composi??es das assembleias e dos componentes das popula??es e dos componentes das comunidades de tr?s ?reas de mata ciliar inseridasno bioma Mata Atl?ntica, munic?pio de Nova Igua?u, regi?o do Tingu?, Estado do Rio de Janeiro, Brasil. Duas ?reas na zona de amortecimento da Reserva Biol?gica do Tingu?, uma ?rea agr?cola, Takume, e uma ?rea de reflorestamento, Canavarro, e uma ?rea no interior da Reserva Biologica do Tingu?, Rebio, considerada como a ?rea mais preservada. O esfor?o amostral foi similar nas tr?s ?reas, com amostragens mensais, totalizando 12 noites em cada ?rea, entre maio de 2011 e abril de 2012. Foram encontradas 22 esp?cies de morcegos Phyllostomidae, 24 esp?cies de moscas Streblidae e cinco de ?caros Spinturnicidae no computo geral das tr?s ?reas. A Rebio apresentou as maiores riquezas de morcegos e ectoparasitos, enquanto que Canavarro apresentou as maiores abund?ncias de indiv?duos e dos ?ndices parasitol?gicos de preval?ncia, intensidade e abund?ncia m?dia de infesta??o. As maiores riquezas da Rebio foram atribu?das ao maior grau de preserva??o da ?rea que permitiu a maior riqueza de morcegos e assim de seus ectoparasitos associados. As maiores abund?ncias, assim como os maiores ?ndices parasitol?gicos do Canavarro foram atribu?dos ? elevada abund?ncia de duas esp?cies de morcegos, Artibeus lituratus e Sturnira lilium. Ambas as esp?cies s?o comumente encontradas infestadas tanto por esp?cimes de Streblidae como por esp?cimes de Spinturnicidae. Os dados refor?am a id?ia de alta especificidade destes ectoparasitos com seus hospedeiros. J? que o parasitismo das ?reas acompanhou as varia??es das assembleias de morcegos, n?o demonstrando varia??es que poderiam estar associadas a outras vari?veis que n?o seus hospedeiros preferenciais.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-05-04T17:50:45Z No. of bitstreams: 1 2014 - Paloma Martins Mendon?a.pdf: 5837915 bytes, checksum: 4380ed0e7adefff59205996c112ae7af (MD5) Made available in DSpace on 2018-05-04T17:50:46Z (GMT). No. of bitstreams: 1 2014 - Paloma Martins Mendon?a.pdf: 5837915 bytes, checksum: 4380ed0e7adefff59205996c112ae7af (MD5) Previous issue date: 2014-10-16 CNPq The Order Diptera is composed by about 153 thousand described species, but it is believed that the number of unknown species is much bigger. Adults and immature of some muscoid dipteran groups have little morphological differences, making the identification process more difficult. Besides, the available dichotomous keys are based on adults, but in the case of myiasis and cadaver colonization, only immature are found. These insects are known pathogen vectors, their larvae parasite humans and animais and they play an important role in forensic entomology. Therefore, the correct identification of these species is very important. This study aims to characterize features of medical, veterinary and forensic important dipteran species using two new approaches. The first chapter uses scanning electron microscopy which gives detailed information on the morphological characters that can help identify the immature forms of the flies. Immatures belonging to three muscoids with forensic and sanitary importance were studied using this methodology: Peckia (Eubaettcheria) cal/usar (Diptera: Sarcophagidae) (larvae and puparia), Luc?lia cuprina (larvae and puparia) e Cachliamyia macellaria (eggs, larvae and puparia) (Diptera: Calliphoridae). The size ofthe chorionic cells as well as the absence of spines at the posterior spiracle allowed to differentiate C. mace/faria from C. haminivarax, both myiasis causing species. The maxillary palp complex composed by a group of papillae inside a cavity seem to be specific to the subgenus Eubaeucheria. The anterior spiracular openings displaced in an irregular row are found exclusively in Sarcophagidae. The filiform spines at the anal segments are found only in the genus Lucilia. In the second chapter, barcading is used to provide a gene sequence database of medical, veterinary and forensically important Calliphoridae flies. This technique is based on the sequencing of a portion of the genome of the mitochondrial cytochrome oxidase l (cal) gene. Nine species of flies were collected in four regions in Brazil, corresponding to 346 specimens. Two other gene fragments ( elongation factor 1 alpha and white) were sequenced for the first time for some species. The barcade proposal was successfully achieved for Hemilucilia segmentaria, Hemi/ucilia semidiaphana, Chlarapracla idiaidea, Cachliamyia macellaria, Chrysamya megacephala and Chrysamya albiceps, for which the intraspecific variation was less than 3%. However, for Lucilia eximia, Chrysamya putaria and Chrysamya rufifacies, this methodology brought incipient results. Intraspecific variation among Lucilia eximia samples was 4.6%, suggesting that the threshold proposed should not be used for ali dipteran species without previous analysis of the studied group. Chrysamya putaria is polyphyletic according to nuclear and mitochondrial genes. In addition, a set of samples grouped with samples of C. albiceps and C. rufifacies. Therefore, more studies need to be clone to better explain these taxonomic problems. A ordem Diptera ? composta por aproximadamente 153 mil esp?cies descritas, mas acredita-se que o n?mero de esp?cies ainda desconhecidas ? muito grande. Alguns grupos de d?pteros musc?ides possuem imaturos e adultos com poucas varia??es morfol?gicas, dificultando muito o processo de identifica??o. Al?m disso, as chaves dicot?micas existentes s?o baseadas nos adultos, entretanto, s?o os imaturos mais encontrados causando mi?ases ou colonizando cad?veres. Estes inselos s?o conhecidos vetores de pat?genos, suas larvas s?o parasitas de humanos e animais e apresentam papel importante como auxilio a entomologia forense. Assim, a correta identifica??o das esp?cies que desempenham estas fun??es ? muito importante. O presente estudo tem como objetivo principal caracterizar esp?cies de d?ptcros mnscoidcs de import?ncia m?dica-veterin?ria e forense, atrav?s da utiliza??o de duas t?cnicas modernas de identifica??o. O primeiro cap?tulo utiliza a microscopia eletr?nica de varredura que permite a visualiza??o e a determina??o de caracteres que possam ser utilizados na identifica??o das esp?cies. Foram estudados imaturos de tr?s esp?cies de d?pteros de duas fam?lias diferentes: Peckia (Eubaettcheria) callusar (Diptera: Sarcophagidae) (larvas e pup?rios), Lucilia cuprina (larvas e pup?rios) e Cachliamyia macellaria (ovos, larvas e pup?rios) (Diptera: Calliphoridae) atrav?s desta t?cnica. O tamanho das c?lulas do exoc?rion permitiu diferenciar entre C. macellaria e C. haminivarax, duas esp?cies causadoras de mi?ases. A aus?ncia de espinhos no espir?culo posterior das larvas de C. macellaria tamb?m a diferencia de C. haminivarax. As papilas do complexo do palpo maxilar em uma cavidade parecem ser espec?ficas do subg?nero Eubaettcheria. As aberturas respirat?rias do espir?culo anterior dispostas em fileiras irregulares s?o exclusivas da fam?lia Sarcophagidae. A presen?a de espinhos filamentosos no ?ltimo segmento larval ? caracter?stica espec?fica do g?nero Lucilia. No segundo cap?tulo, a t?cnica de harcade ? utilizada para a forma??o de um banco de dados contendo sequ?ncias g?nicas de esp?cies de import?ncia m?dica-veterin?ria e forense da fam?lia Calliphoridae. Esta t?cnica baseia-se no sequenciamento de uma por??o do gene mitocondrial citocromo oxidase I (cal). Foram analisadas nove esp?cies de d?pteros, coletadas em quatro regi?es do Brasil, em um total de 346 esp?cimes utilizados. Dois segmentos do genoma nuclear (fator de elonga??o 1 alfa e white) tamb?m foram sequenciados pela primeira vez para algumas esp?cies. A proposta de barcade foi bem sucedida em Hemilucilia segmentaria, Hemilucilia semidiaphana, Chlaraprocta idiaidea, Cachliamyia macellaria, Chrysamya megacephala e Chrysamya albiceps, onde a varia??o intraespec?fica foi menor do que 3%. Entretanto, em Lucilia eximia, Chrysamya putaria e Ch1ysamya rufifacies esta metodologia trouxe resultados incipientes. A varia??o intraespec?fica em Lucilia eximia foi 4,6%, sugerindo que o limiar proposto n?o deve ser aplicado em todos os d?pteros sem an?lise pr?via do grupo estudado. Chrysamya pularia ? uma esp?cie polifi!?tica comprovada tanto pelos genes nucleares quanto mitocondrial, al?m isso, uma parte das amostras forma um agrupamento com as amostras de C. albiceps e C. nijifacies. Assim, acrcditase que mais estudos precisam ser conduzidos com o objetivo de esclarecer melhor estas quest?es taxon?micas.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2020-06-10T22:17:36Z No. of bitstreams: 1 2012 - Gabriel Alves Landulfo.pdf: 1078425 bytes, checksum: 59bea9a07e6f134e771141a4b8803ff3 (MD5) Made available in DSpace on 2020-06-10T22:17:37Z (GMT). No. of bitstreams: 1 2012 - Gabriel Alves Landulfo.pdf: 1078425 bytes, checksum: 59bea9a07e6f134e771141a4b8803ff3 (MD5) Previous issue date: 2012-02-09 Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico, CNPq, Brasil Ornithodoros mimon Kohls, Clifford & Jones, 1969 is an argasid tick, originally described from larvae collected on bats from Bolivia and Uruguay. In Brazil the species is aggressive to humans and animals. Nymphs and adults of O. mimon were collected from the roof of a residence in Araraquara, S?o Paulo, Brazil, whose residents were bitten by ticks. Once in the laboratory, they were fed rabbits and maintained in biological oxygen demand incubator at 27 ?C ? 1 ?C and 90% ? 10% relative humidity. The females, after mating, laid eggs that resulted in larvae that were identified by the original description and also by the paratypes examination (RML 50271-50274) deposited at the United State National Tick Collection, Georgia, GA, USA. The life cycle of this species was obtained through the acquisition of two generations of ticks (F1 and F2) in the laboratory using rodents and rabbits as hosts. The biological parameters of larva, nymph and adult stages of both generations were observed and recorded from infestations in the laboratory hosts. Larvae showed a profile of feeding for days on the host, while the nymphs and adults for few minutes. It was observed molt from first nymphal instar (N1) to second nymphal instar (N2) without blood meal. The species was successfully colonized and its life cycle was elucidated for the first time. Thus, the findings obtained in this study have contributed with information for better knowledge of the species, and provides subsidies for maintenance of the colony in the laboratory. Ornithodoros mimon Kohls, Clifford & Jones, 1969 ? um carrapato argas?deo parasito de Chiroptera, originalmente descrito de larvas coletadas sobre morcegos da Bol?via e Uruguai. No Brasil a esp?cie ? bastante agressiva aos humanos e aos animais. Ninfas e adultos de O. mimon foram coletados do teto de uma resid?ncia em Araraquara, S?o Paulo, Brasil, cujos moradores estavam sendo picados pelos carrapatos. Uma vez em laborat?rio, os exemplares foram alimentados em coelhos da ra?a Nova Zel?ndia e mantidos em estufas de B.O.D. ? 27?C ? 1?C e 90% de umidade. As f?meas, ap?s o acasalamento, realizaram posturas que resultaram em larvas que foram identificadas atrav?s da descri??o original (Kohls et al., 1969) e tamb?m pela compara??o com os par?tipos (RML 50271-50274) depositados na Universidade da Georgia, USA. O ciclo biol?gico desta esp?cie foi obtido atrav?s da aquisi??o de duas gera??es de carrapatos (F1 e F2) em laborat?rio, utilizando roedores e coelhos como hospedeiro. Os par?metros biol?gicos de larva, ninfa e adulto de ambas as gera??es foram observados e registrados a partir de infesta??es nos hospedeiros de laborat?rio. As larvas apresentaram um perfil de alimenta??o por dias no hospedeiro, enquanto que as ninfas e adultos por cerca de minutos. Registrou-se mudan?a de ninfas de primeiro ?nstar (N1) para ninfas de segundo ?nstar (N2) sem repasto sangu?neo. A esp?cie foi colonizada com sucesso e o seu ciclo biol?gico foi elucidado pela primeira vez. Assim, os achados obtidos no presente estudo contribu?ram com informa??es para um melhor conhecimento da esp?cie, al?m de fornecer subs?dios para manuten??o de col?nia em laborat?rio.