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The development of modern agriculture requires the reduction of water and chemical N fertilizer inputs. Increasing the planting density can maintain higher yields, but also consumes more of these restrictive resources. However, whether an increased maize density can compensate for the negative effects of reduced water and N supply on grain yield and N uptake in the arid irrigated areas remains unknown. This study is part of a long-term positioning trial that started in 2016. A split-split plot field experiment of maize was implemented in the arid irrigated area of northwestern China in 2020 to 2021. The treatments included two irrigation levels: local conventional irrigation reduced by 20% (W1, 3,240 m3 ha–1) and local conventional irrigation (W2, 4,050 m3 ha–1); two N application rates: local conventional N reduced by 25% (N1, 270 kg ha–1) and local conventional N (360 kg ha–1); and three planting densities: local conventional density (D1, 75,000 plants ha–1), density increased by 30% (D2, 97,500 plants ha–1), and density increased by 60% (D3, 120,000 plants ha–1). Our results showed that the grain yield and aboveground N accumulation of maize were lower under the reduced water and N inputs, but increasing the maize density by 30% can compensate for the reductions of grain yield and aboveground N accumulation caused by the reduced water and N supply. When water was reduced while the N application rate remained unchanged, increasing the planting density by 30% enhanced grain yield by 13.9% and aboveground N accumulation by 15.3%. Under reduced water and N inputs, increasing the maize density by 30% enhanced N uptake efficiency and N partial factor productivity, and it also compensated for the N harvest index and N metabolic related enzyme activities. Compared with W2N2D1, the N uptake efficiency and N partial factor productivity increased by 28.6 and 17.6% under W1N1D2. W1N2D2 had 8.4% higher N uptake efficiency and 13.9% higher N partial factor productivity than W2N2D1. W1N2D2 improved urease activity and nitrate reductase activity by 5.4% at the R2 (blister) stage and 19.6% at the V6 (6th leaf) stage, and increased net income and the benefit:cost ratio by 22.1 and 16.7%, respectively. W1N1D2 and W1N2D2 reduced the nitrate nitrogen and ammoniacal nitrogen contents at the R6 stage in the 40–100 cm soil layer, compared with W2N2D1. In summary, increasing the planting density by 30% can compensate for the loss of grain yield and aboveground N accumulation under reduced water and N inputs. Meanwhile, increasing the maize density by 30% improved grain yield and aboveground N accumulation when water was reduced by 20% while the N application rate remained constant in arid irrigation areas.

Above- and below-ground interactions play a crucial role in achieving higher yields in intercropping systems. Nonetheless, it remains unclear how these interactions impact intercropping crop growth and regulate interspecific relationships. This study aimed to quantify the impact of above- and below-ground interactions on crop yield by determining the dynamics of dry matter accumulation, photosynthetically active radiation (PAR) transmittance, and leaf area index (LAI) in intercropped wheat and maize. Three below-ground intensities were set for an intercropping system: no root separation (CI: complete interaction below ground), 48 μm nylon mesh separation (PI: partial interaction below ground), and 0.12 mm plastic sheet separation (NI: no interaction below ground). Two densities were set for maize: low (45,000 plants hm−2) and high (52,500 plants hm−2). At the same time, corresponding monoculture treatments were established. The grain yields in the CI and PI treatments were, on average, 23.7% and 13.7% higher than those in the NI treatment at high and low maize densities, respectively. Additionally, the grain yield for high density was 12.3% higher than that of low density in the CI treatment. The dry matter accumulation of intercropped wheat under the CI and PI treatments was, on average, 9.1%, 14.5%, and 9.0% higher than that in the NI treatment at the flowering, filling, and maturity stages, respectively. The dry matter accumulation of intercropped maize at the blister, milk, and physiological maturity stages increased by 41.4%, 32.1%, and 27.8%, respectively, under the CI treatment compared to the NI treatment. The PAR transmittance and LAI of maize at the V6 stage were significantly increased by increasing the intensity of below-ground interactions. This study showed that complete below-ground interaction contributed to a significant increase in the competitiveness of intercropped wheat with respect to maize (Awm) under the high-density maize treatment, especially at the filling stage of wheat. Moreover, the CI treatment enhanced the recovery effects of maize (Rm) after wheat harvesting. Increasing the intensity of below-ground interactions can significantly enhance the Awm and Rm in intercropping systems, favoring the accumulation of crop dry matter mass and light energy utilization to increase system yields.

Background: Liver injury in pregnancy significantly impacts the physical and mental health of pregnant women, and finding a potential therapeutic target is crucial for early prediction and improving adverse pregnancy outcomes. This study aims to examine the relationship between miR-339-5p expression and early diagnosis and pregnancy outcomes in patients experiencing liver injury in pregnancy. Methods: A retrospective study of 63 patients with liver injury in pregnancy. The expression of miR-339-5p in plasma of patients with liver health and liver injury in pregnancy was detected using quantitative real-time polymerase chain reaction (qRT-PCR). The value of miR-339-5p in the diagnosis and pregnancy outcomes of patients with liver injury in pregnancy was evaluated by receiver operating characteristic (ROC) and Cox regression analysis. Results: The alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bile acids (TBA), total bilirubin (TBIL) levels and miR-339-5p expression of patients in the intrahepatic cholestasis of pregnancy (ICP), hemolysis, elevated liver enzymes, and low platelet count (HELLP) and acute fatty liver of pregnancy (AFLP) groups, respectively, were statistically significant compared with those in the healthy control (HC) group (p < 0.05). MiR-339-5p expression was significantly lower in patients with liver injury in pregnancy compared to healthy individuals. This difference could be used to distinguish between healthy individuals and those with liver injury in pregnancy (area under the curve (AUC) = 0.897, 95% confidence interval (95% CI) = 0.843–0.951). In addition, ALT (r = –0.686), AST (r = –0.699) and TBA (r = –0.706) were highly negatively correlated with miR-339-5p expression, respectively. MiR-339-5p can be used as a biomarker of liver injury in pregnancy to predict adverse pregnancy outcomes. Conclusions: MiR-339-5p could potentially be used as a potential molecular marker for early diagnosis of liver injury in pregnancy and the prediction of adverse pregnancy outcomes.

In the Hexi oasis irrigation area, green manure improves the use efficiency of soil, light, heat and precipitation resources during a long fallow period (from the wheat harvest to the later maize sowing). The work aims to determine the optimal utilization patterns of green manure for reducing maize evapotranspiration (ETc) reduction and improvement of grain yield (GY) and crop water productivity (WPc). A two-year field experiment was conducted at a research station in the Shiyang River Basin (Gansu, China) using a completely randomized design with three replications. Five treatments were involved in this study: (i) conventional tillage fallow and leisure without green manures as a control (CT), (ii) tillage with total of green manures incorporated in the soil (TG), (iii) no-tillage with total green manures mulched on soil surface (NTG), (iv) tillage with root incorporated in the soil and aboveground removal of green manures removed (T), (v) no-tillage with aboveground manures removed (NT). The results showed that the soil bulk density was reduced by 1.4–8.9%, and field capacity was increased by 1.0–4.5% under green manure returning. The soil water storage (SWS) at 0–100 cm soil layer was improved during the maize growing season. Compared with CT, maize evapotranspiration (ETc) was significantly reduced by 7.4% due to the significant decline in soil evaporation (E) by 12.8% under the NTG treatment. On average, the maximum root biomass (RBmax) (26.8 g plant–1), aboveground biomass (ABmax) (421.1 g plant–1), GY (15085.5 kg ha−1) and WPc (3.2 kg m−3) of NTG were significantly increased by 79.7%, 25.3%, 34.3% and 39.2%, respectively. Rising RB and falling ineffective ETc contribute to the increase in GY and WPc. For the spring wheat-maize rotation system, NTG is an ideal green manure returning method for achieving high yield and WPc in the arid oasis irrigation area.

Increasing plant density can increase cereal crop yields. However, the physiological and anatomical mechanisms of grain yield increase at high plant densities in maize-based intercropping systems are not well understood. A two-year field experiment was conducted in 2018 and 2019 to investigate grain yield, photosynthetic characteristics, stomatal traits, and leaf anatomy of maize plants in an intercropping system with high plant densities. Two cropping patterns (monocropping and intercropping) and three plant densities (D1, 78,000 plants ha−1; D2, 103,500 plants ha−1; D3, 129,000 plants ha−1) were arranged in a randomized block design. Increasing maize plant density significantly increased maize yield, and intercropping gave a significant yield advantage over monocropping under the same plant density. Intercropping combined with high plant density increased the leaf area and SPAD value of maize, increasing the photosynthesis rates after the harvest of pea. At the twelfth leaf stage, the stomatal density and stomatal area of intercrops combined with medium plant density increased by respectively 10.5% and 18.4% relative to their values for the corresponding density of monocrops. Although leaf thickness of maize was reduced by increasing plant density, the chloroplast number and grana lamella number were higher in intercropping than in monocropping under different plant densities. These positive changes in leaf anatomy resulted in increased photosynthesis, suggesting a physiological basis for the increase in grain yield.

To make the quantitative results of nuclear magnetic resonance (NMR) transverse relaxation (T2) spectrums reflect the type and pore structure of reservoir more directly, an unsupervised clustering method was developed to obtain the quantitative pore structure information from the NMR T2 spectrums based on the Gaussian mixture model (GMM). Firstly, We conducted the principal component analysis on T2 spectrums in order to reduce the dimension data and the dependence of the original variables. Secondly, the dimension-reduced data was fitted using the GMM probability density function, and the model parameters and optimal clustering numbers were obtained according to the expectation-maximization algorithm and the change of the Akaike information criterion. Finally, the T2 spectrum features and pore structure types of different clustering groups were analyzed and compared with T2 geometric mean and T2 arithmetic mean. The effectiveness of the algorithm has been verified by numerical simulation and field NMR logging data. The research shows that the clustering results based on GMM method have good correlations with the shape and distribution of the T2 spectrum, pore structure, and petroleum productivity, providing a new means for quantitative identification of pore structure, reservoir grading, and oil and gas productivity evaluation.

No-till and cereal–legume intercropping have been recognized as favorable cropping practices to increase crop yields while maintaining soil quality in arid and semiarid environments, but the biological mechanisms are poorly understood. The present study aimed to determine the response of yields, soil properties, enzyme activities, and microbial community diversity and composition in mono- and inter-cropping under conventional and no-tillage conditions. We initiated a field experiment in Wuwei, a typical arid area of China, in 2014. Soil was sampled in August 2022 and, yields, soil properties, enzyme activities, and the microbial community diversity and composition were determined in the maize and pea strips in inter- and mono-cropping systems. Results revealed that the maize and pea strips in the no-till intercropping significantly increased yields, total and organic carbon stocks, decreased NO3−-N, and obtained the highest total and organic P in the soil. No-tillage significantly enhanced the Shannon index and Pielou evenness of the bacterial community and total microbial community over conventional tillage, with the α-diversity of the bacterial community and total microbial community distinctly higher in the NTIM treatment than in the CTIM treatment. The α-diversity of the total microbial community was significantly related to yield, soil IC and OC, and the α-diversity of the archaea community was significantly related to soil TC, TC/TP, TN/TP, and BX. Meanwhile, the α-diversity of the eukaryote community was significantly related to soil yield, soil TC/TP. Both no-tillage and intercropped maize significantly increased the abundance of archaea phylum Thaumarchaeota and bacterial phylum Nitrospirae, and were significantly positively associated with soil OC and NH4+-N, benefiting nitrogen fixation of intercropped pea from the atmosphere under the no-tillage cereal/legume intercropping. No-till intercropping was conducive to the accumulation of organic carbon, while decreasing the abundance of Proteobacteria, Acidobacteria, and Verrucomicrobia. Limited soil enzyme activities (ACP, ALP, DP, NAG, BG, AG, CB) led to decreases in organic carbon turnover and utilization. Intercropping altered soil microbial community diversity and composition due to changes in soil properties and enzyme activities. These findings suggest that no-tilled cereal–legume intercropping is a sustainable cropping practice for improving soil properties and enhancing microbial (archaea, bacterial, eukaryota) diversity, but the persistence is not conducive to rapid turnover of soil nutrients due to limited enzyme activities.

Abstract Intercropping increases the grain yield to feed the ever-growing population in the world by cultivating two crop species on the same area of land. It has been proven that N-fertilizer postponed topdressing can boost the productivity of cereal/legume intercropping. However, whether the application of this technology to cereal/cereal intercropping can still increase grain yield is unclear. A field experiment was conducted from 2018 to 2020 in the arid region of northwestern China to investigate the accumulation and distribution of dry matter and yield performance of wheat/maize intercropping in response to N-fertilizer postponed topdressing application. There were three N application treatments (referred as N1, N2, N3) for maize and the total amount were all 360 kg N ha−1. N fertilizer were applied at four time, i.e. prior to sowing, at jointing stage, at pre-tasseling stage, and at 15 days post-silking stage, respectively. The N3 treatment was traditionally used for maize production and allocations subjected to these four stages were 2:3:4:1. The N1 and N2 were postponed topdressing treatments which allocations were 2:1:4:3 and 2:2:4:2, respectively. The results showed that the postponed topdressing N fertilizer treatments boosted the maximum average crop growth rate (CGR) of wheat/maize intercropping. The N1 and N2 treatments increased the average maximum CGR by 32.9% and 16.4% during the co-growth period, respectively, and the second average maximum CGR was increased by 29.8% and 12.6% during the maize recovery growth stage, respectively, compared with the N3 treatment. The N1 treatment was superior to other treatments, since it increased the CGR of intercropped wheat by 44.7% during the co-growth period and accelerated the CGR of intercropped maize by 29.8% after the wheat had been harvested. This treatment also increased the biomass and grain yield of intercropping by 8.6% and 33.7%, respectively, compared with the current N management practice. This yield gain was primarily attributable to the higher total translocation of dry matter. The N1 treatment increased the transfer amount of intercropped wheat by 28.4% from leaf and by 51.6% from stem, as well as increased the intercropped maize by 49.0% of leaf, 36.6% of stem, and 103.6% of husk, compared to N3 treatment, respectively. Integrated the N fertilizer postponed topdressing to the wheat/maize intercropping system have a promotion effect on increasing the translocation of dry matter to grain in vegetative organs. Therefore, the harvest index of intercropped wheat and maize with N1 was 5.9% and 5.3% greater than that of N3, respectively. This demonstrated that optimizing the management of N fertilizer can increase the grain yield from wheat/maize intercropping via the promotion of accumulation and translocation of dry matter.

Strategies to reduce carbon emissions have been a hotspot in sustainable agriculture production. The delayed N fertilizer application had the potential to reduce carbon emissions in pea (Pisum sativum L.)/maize (Zea mays L.) intercropping, but its microbial mechanism remains unclear. In this study, we investigated the effects of delayed N fertilizer application on CO2 emissions and soil microbial diversity in pea/maize intercropping. The soil respiration (Rs) rates of intercropped pea and intercropped maize were decreased by 24.7% and 25.0% with delayed application of N fertilizer, respectively. The total carbon emissions (TCE) of the pea/maize intercropping system were also decreased by 21.1% compared with that of the traditional N fertilizer. Proteobacteria, Bacteroidota, and Chloroflexi were dominant bacteria in pea and maize strips. Heatmap analysis showed that the soil catalase activity at the pea flowering stage and the soil ΝΗ4+-Ν at the maize silking stage contributed more to the variations of bacterial relative abundances than other soil properties. Network analysis demonstrated that Rs was positively related to the relative abundance of Proteobacteria and Bacteroidota, while negatively related to the relative abundance of Chloroflexi in the pea/maize intercropping system. Overall, our results suggested that the delayed application of N fertilizer combined with the pea/maize intercropping system altered soil bacterial community diversity, thereby providing novel insights into connections between soil microorganisms and agricultural carbon emissions.

Excessive nitrogen (N) fertilizer has threatened the survivability and sustainability of agriculture. Improving N productivity is promising to address the above issue. Therefore, the field experiment, which investigated the effect of no-till and N fertilizer reduction on water use and N productivity of spring wheat (Triticum aestivum L.), was conducted at Wuwei experimental station in northwestern China. There were two tillage practices (conventional tillage, CT; and no-till with previous plastic film mulching, NT) and three N fertilizer rates (135 kg N ha–1, N1; 180 kg N ha–1, N2; and 225 kg N ha–1, N3). The results showed that NT lowered soil evaporation (SE) by 22.4% while increasing the ratio of transpiration to evapotranspiration (T/ET) by 13.6%, compared with CT. In addition, NT improved the total N accumulation by 11.5% and enhanced N translocation (NT) quantity, rate, and contribution by a range of 6.2–23.3%. Ultimately, NT increased grain yield (GY), N partial factor productivity, and N harvest index by 13.4, 13.1, and 26.0%, respectively. Overall, N1 increased SE (13.6%) but decreased T/ET (6.1%) compared with N3. While, N2 enhanced NT quantity, rate, and contribution by a range of 6.0–15.2%. With the integration of NT, N2 achieved the same level of GY and N harvest index as N3 and promoted N partial factor productivity by 11.7%. The significant positive correlation of NT relative to T/ET and GY indicated that improving T/ET was essential for achieving higher NT. Therefore, we concluded that no-till coupled with N fertilizer rate at 180 kg N ha–1 was a preferable management option to boost the N productivity of spring wheat in arid areas.

Abstract Crop yield is limited by water and nitrogen (N) availability. However, in Hexi Corridor of northwestern China, water scarcity and excessive fertilizer N in wheat (Triticum aestivum L.) production causes serious conflicts between water and N supply and crop demand. A field experiment was conducted from 2016 to 2018 to evaluate whether reducing of irrigation and fertilizer N will reduce grain yield of wheat. There were two irrigation quotas (192 and 240 mm) and three fertilizer N rates (135, 180, and 225 kg N ha−1). The results showed that reducing irrigation to 192 mm and N rate to 180 kg N ha−1 reduced water uptake, water uptake efficiency, and N uptake of spring wheat as compared to local practice (i.e., 240 mm irrigation and 225 kg N ha−1 fertilizer). Whereas, it improved water and N utilization efficiency, and water and N productivity. Consequently, the irrigation and N rate reduced treatment achieved the same quantity of grain yield as local practice. The path analysis showed that interaction effect between irrigation and N fertilization may attributable to the improvement of grain yield with lower irrigation and N rate. The enhanced water and N utilization allows us to conclude that irrigation quota at 192 mm coupled with fertilizer N rate at 180 kg N ha−1 can be used as an efficient practice for wheat production in arid irrigation areas.

Nitrogen fertilizers play a key role in crop production to meet global food demand. Inappropriate application of nitrogen fertilizer coupled with poor irrigation and other crop management practices threaten agriculture and environmental sustainability. Over application of nitrogen fertilizer increases nitrogen gas emission and nitrate leaching. A field experiment was conducted in China’s oasis irrigation area in 2018 and 2019 to determine which nitrogen rate, plant density, and irrigation level in sole maize (Zea mays L.) cropping system reduce ammonia emission and nitrate leaching. Three nitrogen rates of urea (46-0-0 of N-P2O5-K2O), at (N0 = 0 kg N ha−1, N1 = 270 kg N ha−1, and N2 = 360 kg N ha−1) were combined with three plant densities (D1 = 75,000 plants/ha−1, D2 = 97,500 plants/ha−1, and D3 = 120,000 plants/ha−1) with two irrigation levels (W1 = 5,250 m3/hm2 and W2 = 4,740 m3/hm2) using a randomized complete block design. The results showed that, both the main and interaction effects of nitrogen rate, plant density, and irrigation level reduced nitrate leaching (p < 0.05). In addition, irrigation level × nitrogen rate significantly (p < 0.05) reduced ammonia emission. Nitrate leaching and ammonia emission decreased with higher irrigation level and higher plant density. However, high nitrogen rates increased both nitrate leaching and ammonia emission. The study found lowest leaching (0.35 mg kg−1) occurring at the interaction of 270 kg N ha−1 × 120,000 plants/ha−1 × 4,740 m3/hm2, and higher plant density of 120,000 plants/ha−1 combined with 0 kg N ha−1 and irrigation level of 5,250 m3/hm2 recorded the lowest ammonia emission (0.001 kg N)−1. Overall, ammonia emission increased as days after planting increased while nitrate leaching decreased in deeper soil depths. These findings show that, though the contributory roles of days after planting, soil depth, amount of nitrogen fertilizer applied and year of cultivation cannot be undermined, it is possible to reduce nitrate leaching and ammonia emission through optimized nitrogen rate, plant density and regulated irrigation for agricultural and environmental sustainability.

To some extent, the photosynthetic traits of developing leaves of maize are regulated systemically by water and nitrogen. However, it remains unclear whether photosynthesis is systematically regulated via water and nitrogen when maize crops are grown under close (high density) planting conditions. To address this, a field experiment that had a split-split plot arrangement of treatments was designed. Two irrigation levels on local traditional irrigation level (high, I2, 4,050 m3 ha−1) and reduced by 20% (low, I1, 3,240 m3 ha−1) formed the main plots; two levels of nitrogen fertilizer at a local traditional nitrogen level (high, N2, 360 kg ha−1) and reduced by 25% (low, N1, 270 kg ha−1) formed the split plots; three planting densities of low (D1, 7.5 plants m−2), medium (D2, 9.75 plants m−2), and high (D3, 12 plants m−2) formed the split-split plots. The grain yield, gas exchange, and chlorophyll a fluorescence of the closely planted maize crops were assessed. The results showed that water–nitrogen coupling regulated their net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), quantum yield of non-regulated non-photochemical energy loss [Y(NO)], actual photochemical efficiency of PSII [Y(II)], and quantum yield of regulated non-photochemical energy loss [Y(NPQ)]. When maize plants were grown at low irrigation with traditional nitrogen and at a medium density (i.e., I1N2D2), they had Pn, Gs, and Tr higher than those of grown under traditional treatment conditions (i.e., I2N2D1). Moreover, the increased photosynthesis in the leaves of maize in the I1N2D2 treatment was mainly caused by decreased Y(NO), and increased Y(II) and Y(NPQ). The coupling of 20%-reduced irrigation with the traditional nitrogen application boosted the grain yield of medium density-planted maize, whose Pn, Gs, Tr, Y(II), and Y(NPQ) were enhanced, and its Y(NO) was reduced. Redundancy analysis revealed that both Y(II) and SPAD were the most important physiological factors affecting maize yield performance, followed by Y(NPQ) and NPQ. Using the 20% reduction in irrigation and traditional nitrogen application at a medium density of planting (I1N2D2) could thus be considered as feasible management practices, which could provide technical guidance for further exploring high yields of closely planted maize plants in arid irrigation regions.

Leaf photophysiological characteristics are the main indexes that determine crop yield formation. However, it remains unclear whether photosynthesis is systematically regulated via the cropping pattern and nitrogen supply when maize crops are planted with a high density. So, a field experiment that had a three-factor split-plot arrangement of treatments was conducted from 2020 to 2021. The main plot was two cropping patterns that included the sole cropping of maize and wheat–maize intercropping. The split plot had two nitrogen application rates: a traditional nitrogen application rate (N2, 360 kg ha−1) and one reduced by 25% (N1, 270 kg ha−1) for maize. The split–split plot had three planting densities: a traditional density (M1, 78,000 plant ha−1), a medium density (M2, 10,400 plant ha−1), and a high density (M3, 129,000 plant ha−1) for sole maize; the corresponding densities of intercropped maize were 45,000, 60,000, and 75,000 plant ha−1, respectively. The grain yield, the photosynthetic traits, and chlorophyll a fluorescence of the maize were assessed. The results showed that a 25% nitrogen reduction and dense planting had a negative impact on the individual maize’s photosynthesis. However, intercropping could alleviate these drawbacks. When the maize was grown in the intercropping system at a lower nitrogen level and a medium planting density (IN1M2), the photosynthetic traits were better or similar to those of the traditional treatment (SN2M1) at the reproductive growth stage. Moreover, IN1M2 improved the light energy distribution among photochemistry, photo-protective and heat dissipation process of maize compared with SN2M1. A grey relation analysis demonstrated that the Pn and Tr of the individual maize played the most significant role in the group’s productivity. Thus, the IN1M2 treatment achieved the highest grain yield and can be recommended as a feasible agronomic practice in oasis-irrigated regions.

Plastic is a valuable mulching measure for increasing crop productivity in arid environments; however, little is known about the main mechanism by which this valuable technology actuates spatial–temporal changes in soil hydrothermal effect. So a 3-year field experiment was conducted to optimize soil hydrothermal effect of maize field with three plastic mulched management treatments: (1) no tillage with plastic re-mulching (NM), (2) reduced tillage with plastic mulching (RM), and (3) conventional tillage with annual new plastic mulching (CM). The results showed that NM treatment increased soil water content by 6.6–8.4% from maize sowing to seedling stage, than did CM, and it created a good soil moisture environment for sowing of maize. Also, NM had greater soil water content by 4.8–5.6% from maize silking to early-filling stage than had CM, and it made up for the abundant demand of soil moisture for the vigorous growth of maize filling stage. The NM treatment increased water consumption (WC) before maize big-flare stage, decreased WC from big-flare to early-filling stage, and increased WC after early-filling stage. So NM treatment effectively coordinated water demand contradiction of maize at entire growing season. NM decreased soil accumulated temperature (SAT) by 7.0–13.0% at maize sowing to early-filling stage than did CM, but NM had little influence on the SAT during filling stage. In particular, the treatment on NM had smaller absolute values of air–soil temperature differences than RM and CM treatments during maize filling stage, indicating that NM treatment maintains the relative stability of soil temperature for ensuring grain filling of maize. The NM treatment allowed the maize to grow in a suitable hydrothermal status and still maintained high yield. In addition, NM treatment obtained higher net income and rate of return by 6.4–11.0% and 44.1–54.5%, respectively, than did CM, because NM treatment mainly decreased the input costs for plastic and machine operations. Therefore, the NM treatment can be recommended as a promising technique to overcome simultaneous heat stress and water shortage in arid environments.

Nitrogen is a key factor in maize (Zea mays L.) grain and biomass production. Inappropriate application with sub-optimum plant density and irrigation can lead to low productivity and inefficient use. A two-year field experiment was conducted to determine which nitrogen rate, plant density, and irrigation level optimize grain, biomass yield, and water use efficiency. Three nitrogen rates of urea (46–0–0 of N–P2O5–K2O) (N0 = 0 kg N ha−1, N1 = 270 kg N ha−1, and N2 = 360 kg N ha−1), with three maize densities (D1 = 75,000 plants ha−1, D2 = 97,500 plants ha−1, and D3 = 120,000 plants ha−1), and two irrigation levels (W1 = 5250 m3/hm2 and W2 = 4740 m3/hm2) were investigated. The results show that both grain and biomass yields were affected by the main factors. The interaction between nitrogen rate and irrigation level significantly (p < 0.001) affected grain yield but not biomass. It was observed that the grain yield increased correspondingly with nitrogen rate and plant density, while it decreased as the irrigation level increased. Water use efficiency was significantly (p < 0.001) affected by the main factors and their interactions. Nevertheless, water use efficiency was highest at (5250 m3/hm2) × 270 kg N ha−1; × 360 kg N ha−1 × 120,000 plants ha−1 and increased from 62% to 68%. In addition, the highest biomass yield was recorded at 5250 m3/hm2 × 270 kg N ha−1; × 360 kg N ha−1 × 120,000 plants ha−1 while the interaction of either irrigation level with 0 and 270 kg ha−1 or 97,500 and 120,000 plants ha−1 yielded the lowest water use efficiency. Thus, optimized nitrogen rates, plant density, and alternate irrigation levels can support optimum grain and biomass yields. It can also improve nitrogen and water use efficiency in maize production.

Translocation of carbon (C) and nitrogen (N) from vegetative tissues to the grain sinks is critical for grain yield (GY). However, it is unclear how these processes respond to crop management practices when two crops are planted in relay-planting system. In this study, we characterized the C and N accumulation and translocation and their effects on yield formation in a pea (Pisum sativum L.)-maize (Zea mays L.) relay-planting system under different levels of source availabilities. Field experiment was conducted at Wuwei, northwest China, in 2012, 2013, and 2014. Two N fertilizer rates (low – N0 and high – N1) and three maize plant densities (low – D1, medium – D2, and high – D3) were designed to create the different levels of source availabilities. During the co-growth period, the rate of C accumulation in intercropped maize was 7.4–10.8%, 13.8–22.9%, and 13.5–32.0% lower than those in monoculture maize, respectively, under the D1, D2, and D3 treatments; however, after pea harvest, these values were 1.1–23.7%, 33.5–78.9%, and 36.8–123.7% greater than those in monoculture maize. At maturity, intercropped maize accumulated 11.4, 11.5, and 19.4% more N than monoculture maize, respectively, under the D1, D2, and D3 treatments. Compared to the monoculture crops, intercropped pea increased C accumulation in stems by 40.3% with N-application and by 19.5% without N application; intercropping maize increased these values by 16 and 11%, respectively. Overall, increasing N fertilization improved the rates of C and N remobilization from the vegetative tissues to the grain sinks across the different density treatments. In intercropped maize, the stems contributed 22, 33, and 44% more photosynthate to the grain sinks than the leaves, respectively, under the D1, D2, and D3 treatments. Quantitative assessments showed that the enhanced C and N remobilization due to high N fertilization and high plant density led to an increase of GY in the intercropping system by 35% compared with monoculture. We conclude that the enhanced productivity in maize-pea intercropping is a function of the source availability which is regulated by plant density and N fertilization.

A new fluid identification method by constructing water spectrum based on NMR logging was put forward after the limitations of existing nuclear magnetic resonance (NMR) fluid identification methods were analyzed. At present, differential spectrum method (DSM) and shifted spectrum method (SSM) of NMR logging are commonly used fluid identification methods. Due to the effects of fluid properties and pore structures, however, their coincidence rates of fluid identification are lower. A new fluid identification method named water spectrum construction method was developed in this study. Based on the existing acquisition mode of NMR logging, T2 (transverse relaxation time) spectrum of long waiting time and long echo spacing in completely watered conditions was constructed from the T2 spectrum which was measured in the mode of long waiting time and short echo spacing. And then, the types of fluids in reservoirs were identified by comparing the measured T2 spectrum with the constructed water spectrum. This new method was applied in Nanpu sag, Bohai Bay Basin for identifying oil layers, oil-water layers, water layers, gas layers and low-resistivity oil layers. It is demonstrated that based on the water spectrum construction method, the coincidence rate of fluid identification caused by pore structures is increased and fluid identification capacity of NMR logging is improved. Water spectrum construction method is prospective for fluid identification and evaluation of complex reservoirs. Key words: NMR logging, water spectrum method, fluid identification technique, complex reservoirs, fluid identification coincidence rate

Nitrogen (N) fixation is essential in the development of sustainable agriculture, but nodulation of legumes is usually inhibited by N fertilization. In this study, we evaluated the increased density of intercropped maize (Zea mays L.) as a means to alleviate the inhibitory effect of soil mineral N on intercropped pea (Pisum sativum L.) and improve system performance. A field experiment was conducted in the Hexi Corridor region of northwestern China from 2012 to 2014. The experiment consisted of monoculture pea, monoculture maize, and a pea/maize strip-intercropping system. Two levels of N fertilization were evaluated in both cropping systems during the co-growth period of intercropping, i.e., 0 kg N ha−1 (N0) and 135 kg N ha−1 (N1), and three maize densities were evaluated with both levels of N fertilization in the intercropping system, i.e., 45,000 plants ha−1 (D1), 52,500 plants ha−1 (D2), and 60,000 plants ha−1 (D3). The application of N reduced the number of nodules of intercropped pea by 135% at D1 and by 9% at D2 compared to no application of N, in all the years examined. The alleviation of the inhibitory effect of soil mineral N on the nodulation of intercropped pea (Cis) was calculated as the percentage increase in nodulation with intercropping relative to monoculture for a given level of N fertilization. With the application of N, Cis was improved by increased intercropped maize density (D3 > D2 > D1) at all stages. The internal efficiency of nitrogen (IEN) of pea was improved with intercropping and, on average, was 19% and 12% greater at D3 than at D1 and D2, respectively. These results demonstrate that increased maize density can alleviate the inhibitory effect of soil N on the nodulation of pea and sustain the productivity of maize/pea intercropping while reducing N fertilizer requirements in arid regions.

Sustainable intensification of pulses needs reduced input of nitrogen (N) fertilizer with enhanced crop nutritional quality and yield. Therefore, increasing N harvest in grains (sink organs) by improving N remobilization is of key importance. Previous research has shown that a lower dose of N fertilizer effectively increases the rate of N remobilization, while intercropping improves the grain N concentration in pea (Pisum sativum L.). However, it is unknown whether intercropping can facilitate this N fertilizer effect to increase N remobilization, and thereby enhance the N harvest index (NHI). In this study, we determined N allocation among different organs of pea plants, N translocation from leaf and stem tissues to pods, N2 fixation, N utilization efficiency, and NHI of pea plants grown alone or intercropped with maize (Zea mays L.) with different N fertilization treatments in a field experiment in northwestern China from 2012 to 2014. A base application of 90 kg N ha−1 at sowing and top-dress application of 45 kg N ha−1 at flowering integrated with maize–pea intercropping increased N allocation to pod tissues, N translocation to grains, and NHI of pea plants. Compared with the application of 90 kg N ha−1 at sowing and 135 kg N ha−1 top-dressed at flowering, reducing the top-dress application of N fertilizer to 45 kg N ha−1 increased N allocation to intercropped pea plants by 8%. Similarly, N translocation to grains from leaf and stem tissues was increased by 37.9 and 43.2%, respectively, enhancing the NHI by 40.1%. A positive correlation between N2 fixation and NHI was observed, implying that N2 fixation improves N concentration in grain sinks. Thus, our data show that growing pulses in an intercropping system with reduced N fertilization are essential for maximizing N translocation, improving nutritional quality, and preventing the loss of N through leaching, thereby avoiding potential groundwater contamination.

Intercropping is considered a promising system for boosting crop productivity. However, intercropping usually requires higher inputs of resources that emit more CO2. It is unclear whether an improved agricultural pattern could relieve this issue and enhance agricultural sustainability in an arid irrigation area. A field experiment using a well-designed agricultural practice was carried out in northwest China; reduced tillage, coupled with wheat straw residue retention measures, was integrated with a strip intercropping pattern. We determined the crop productivity, water use, economic benefits, and carbon emissions (CEs). The wheat-maize intercropping coupled with straw covering (i.e., NTSI treatment), boosted grain yield by 27–38% and 153–160% more than the conventional monoculture of maize and wheat, respectively, and it also increased by 9.9–11.9% over the conventional intercropping treatment. Similarly, this pattern also improved the water use efficiency by 15.4–22.4% in comparison with the conventional monoculture of maize by 45.7–48.3% in comparison with the conventional monoculture of wheat and by 14.7–15.9% in comparison with the conventional intercropping treatment. Meanwhile, NTSI treatment caused 7.4–13.7% and 37.0–47.7% greater solar energy use efficiency than the conventional monoculture of maize and wheat, respectively. Furthermore, the NTSI treatment had a higher net return (NR) by 54–71% and 281–338% and a higher benefit per cubic meter of water (BPW) by 35–51% and 119–147% more than the conventional monoculture of maize and wheat, respectively. Similarly, it increased the NR and BPW by 8–14% and 14–16% in comparison with the conventional intercropping treatment, respectively. An additional feature of the NTSI treatment is that it reduced CEs by 13.4–23.8% and 7.3–17.5% while improving CE efficiency by 62.6–66.9% and 23.2–33.2% more than the conventional monoculture maize and intercropping treatments, respectively. We can draw a conclusion that intercropping maize and wheat, with a straw covering soil surface, can be used to enhance crop production and NRs while effectively lowering CO2 emissions in arid oasis irrigation region.

Taking the Triassic Chang 7 Member tight reservoirs of the Yanchang Formation in the Ordos Basin as study object, transverse relaxation simulation was made using nuclear magnetic resonance (NMR) experimental data, CT images and random-walker algorithm to evaluate microscopic pore-throats of the tight reservoirs. The smoothing degree of well logging curves was utilized to evaluate sand structure of the tight reservoirs; and through multi-well analysis and the calibration of well testing data, a grading chart of tight reservoir productivity was established based on sand structure. Reservoir quality was judged and analyzed by pore level: Transverse surface relaxivity ρ2 was obtained by comparing transverse relaxation time T2 of numerical simulation and that of NMR experiment, and the distribution of pore-throat radius was obtained according to the corrected NMR logging curves. The study shows there is an obvious correlation between productivity and sand structure of Chang 7 tight reservoirs. The more homogeneous the distribution of lithology, physical property and oiliness of the massive sand, and the smoother the well logging curves, the more likely the high oil yield will occur. Based on well logging evaluation and grading chart of productivity of tight reservoirs, sweet spots in well area W of Jiyuan Oilfield in Ordos Basin were identified, the comparison of the evaluation results and well testing data shows the accuracy rate is as high as 94.7%. Key words: tight oil, well logging evaluation, microscopic pore-throat distribution, sand structure, sweet spot distribution, Ordos Basin

Characterization of pore throat size distribution (PTSD) in tight sandstones is of substantial significance for tight sandstone reservoirs evaluation. High-pressure mercury intrusion (HPMI) and nuclear magnetic resonance (NMR) are the effective methods for characterizing PTSD of reservoirs. NMR T2 spectra is usually converted to mercury intrusion capillary pressure for PTSD characterization. However, the conversion is challenging in tight sandstones due to tiny pore throat sizes. In this paper, the linear conversion method and the nonlinear conversion method are investigated, and the error minimization method and the least square method are proposed to calculate the conversion coefficients of the linear conversion method and the nonlinear conversion method, respectively. Finally, the advantages and disadvantages of these two different conversion methods are discussed and compared with field case study. The research results show that the average linear conversion coefficients of the 20 tight sandstone core plugs collected from Yanchang Formation, Ordos Basin of China is 0.0133 μm/ms; the average nonlinear conversion coefficient is 0.0093 μm/ms and the average nonlinear conversion exponent is 0.725. Although PTSD converted from NMR spectra by the nonlinear conversion method is wider than that obtained from linear conversion method, the nonlinear conversion method can retain the characteristic of bi-modal distribution in PTSD.

Based on current acquisition modes of MRIL-Prime NMR logging tool, 2D NMR signals could be obtained by the combination of logging data from different modes, then the fluid properties in complicated reservoirs could be distinguished by 2D diffusion-relaxation NMR logging data distribution of pore fluids, generated by multi-echotrain joint inversion. In comparison with 1D NMR logging, this method could increase fluid information in diffusion regime, separate oil, gas and water signals in 2D space and enhance the identification capacity of fluid properties from NMR logging. The 2D NMR logging in the multi-echowave interval was applied in the oil pays in Well A and the water layers in Well B in the Nanpu Sag by MRIL-Prime tool, and the interpretation matches the well testing result. It indicates that 2D NMR logging has advantages on the identification of light oil, and fluids in macropore reservoirs than 1D NMR logging. Key words: 2D NMR logging, acquisition mode, joint multi-echotrain inversion, diffusion coefficient

Achieving the green development of agriculture requires the reduction of chemical nitrogen (N) fertilizer input. Previous studies have confirmed that returning green manure to the field is an effective measure to improve crop yields while substituting partial chemical N fertilizer. However, it remains unclear how to further intensify the substituting function of green manure and elucidate its underlying agronomic mechanism. In a split-plot field experiment in spring wheat, different green manures returned to the field under reduced chemical N supply was established in an oasis area since 2018, in order to investigate the effect of green manure and reduced N on grain yield, N uptake, N use efficiency (NUE), N nutrition index, soil organic matter, and soil N of wheat in 2020-2022. Our results showed that mixed sown common vetch and hairy vetch can substitute 40% of chemical N fertilizer without reducing grain yield or N accumulation. Noteworthily, mixed sown common vetch and hairy vetch under reduced N by 20% showed the highest N agronomy efficiency and recovery efficiency, which were 92.0% and 46.0% higher than fallow after wheat harvest and conventional N application rate, respectively. The increase in NUE of wheat was mainly attributed to mixed sown common vetch and hairy vetch, which increased N transportation quantity and transportation rate at pre-anthesis, enhanced N harvest index, optimized N nutrition index, and increased activities of nitrate reductase and glutamine synthetase of leaf, respectively. Meanwhile, mixed sown common vetch and hairy vetch under reduced N by 20% improved soil organic matter and N contents. Therefore, mixed sown common vetch and hairy vetch can substitute 40% of chemical N fertilizer while maintaining grain yield and N accumulation, and it combined with reduced chemical N by 20% or 40% improved NUE of wheat via enhancing N supply and uptake. [ABSTRACT FROM AUTHOR]

To improve light harvest and plant structural support under low light intensity, it is useful to investigate the effects of different ratios of blue light on petiole and stem growth. Two true leaves of soybean seedlings were exposed to a total light intensity of 200 µmol m-2 s-1, presented as either white light or three levels of blue light (40 µmol m-2 s-1, 67 µ mol m-2 s-1 and 100 µmol m-2 s-1) for 15 days. Soybean petioles under the low blue light treatment upregulated expression of genes relating to lignin metabolism, enhancing lignin content compared with the white light treatment. The low blue light treatment had high petiole length, increased plant height and improved petiole strength arising from high lignin content, thus significantly increasing leaf dry weight relative to the white light treatment. Compared with white light, the treatment with the highest blue light ratio reduced plant height and enhanced plant support through increased cellulose and hemicellulose content in the stem. Under low light intensity, 20% blue light enhanced petiole length and strength to improve photosynthate biomass; whereas 50% blue light lowered plants' centre of gravity, preventing lodging and conserving carbohydrate allocation. [ABSTRACT FROM AUTHOR]