Search

Your search keyword '"Falco, Aura"' showing total 10 results
Start Over Author "Falco, Aura"
10 results on '"Falco, Aura"'

7. Additional file 1: Table S1. of A cluster of KPC-2 and VIM-2-producing Klebsiella pneumoniae ST833 isolates from the pediatric service of a Venezuelan Hospital

Author

Falco, Aura, Yusibeska Ramos, Franco, Esther, AlegrĂ­A GuzmĂĄn, and Takiff, Howard

Subjects
animal structures, parasitic diseases, geographic locations
Abstract

Oligonucleotides used for PCR of carbapenemase-resistant Klebsiella pneumoniae isolates from a Hospital in Venezuela. (DOC 77 kb)

Published
2016
Full Text
View/download PDF

9. Isolation and identification of strains belonging to the Bifidobacterium genus found in fermented yoghurt type milk products

Author

Pérez, Indira, Falco, Aura, Tapia, María Soledad, and Alonso, Guillermina

Subjects
caracterización microbiológica, probióticos, Bifidobacterium, ERIC-PCR, REP-PCR, yoghurt, yogur, probiotic, microbiological characterization
Abstract

La identificación de microorganismos probióticos del género Bifidobacterium es de gran importancia por su uso como suplemento que favorece la salud del consumidor. En Venezuela son pocos los estudios sobre caracterización microbiológica de estas bacterias y no existen métodos oficiales para su estudio en alimentos. Esta investigación reporta la estandarización de técnicas microbiológicas y moleculares para el aislamiento e identificación de bifidobacterias aisladas de dos productos tipo yogur, I con probiótico y II sin probiótico. Se analizaron 10 muestras de cada yogur, una por semana, aislando 3 colonias por muestra. Los resultados mostraron que de los 60 aislados analizados, 27 colonias del Yogur I y 11 del Yogur II concordaron con las características de bifidobacterias. Se comparó el crecimiento bacteriano en dos medios de cultivo (MRS-m, RCA), sembrando por profundidad en placas y en tubos Miller-Pricket, obteniéndose mejores resultados con el medio MRS-m y las siembras por profundidad en tubos. De las extracciones de ADN se obtuvieron los patrones de ERIC-PCR y REP-PCR, determinándose que 34 aislados eran clones indistinguibles, mostrando el patrón de B. lactis utilizado como control positivo. Esta metodología puede ser utilizada por la industria y los entes encargados del control de la calidad de los productos probióticos The identification of probiotic microorganisms belonging to the Bifidobacterium genus is very important due to their use as supplements favorable for consumer’s health. In Venezuela there have been few studies of the microbiological characterization of these bacteria and there are no official methods for their study in food. This investigation reports the standardization of microbiological and molecular techniques for the isolation and identification of bifidobacteria isolated from two yoghurt type products: I with probiotic and II without probiotic. Ten samples from each yoghurt type product were analyzed, one per week, and 3 colonies were isolated per sample. Results showed that of the 60 isolates analyzed, 27 colonies of Yoghurt I and 11 of Yoghurt II coincided with the characteristics of bifidobacteria. Bacterial growth was compared in two culture media (MRS-m, RCA), inoculating in-depth in plates and Miller-Pricket tubes; the best results were obtained with MRS-m medium and in-depth inoculations in tubes. By DNA extraction we obtained ERIC-PCR and REP-PCR patterns, determining that 34 isolates were indistinguishable clones, showing the same pattern of the B. lactis used as positive control. This methodology can be used by the industry and the institutions in charge of quality control of probiotic products

Published
2012

10. Molecular epidemiology of KPC-producing Klebsiella pneumoniae isolated from patients in two public hospitals in Carabobo and Zulia states, Venezuela.

Author

Falco A, Barrios Y, Torres L, Sandrea L, and Takiff H

Subjects
Hospitals, Public, Humans, Klebsiella Infections microbiology, Klebsiella pneumoniae isolation & purification, Molecular Epidemiology, Venezuela, beta-Lactamases genetics, Klebsiella pneumoniae enzymology, Klebsiella pneumoniae genetics, beta-Lactamases biosynthesis
Abstract

Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria (K. pneumonia carbapenemase) are the most important causative agents of nosocomial infections worldwide. These isolates have been identified in Venezuela, but little is known about their local spread. The aim of this study was to perform molecular epidemiology of KPC-producing K. pneumoniae isolated from two public hospitals in the Carabobo and Zulia states of Venezuela. Thirty-two K. pneumoniaei solates, phenotypically classified as KPC producers were subjected to PCR to detect the presence of blaKPC genes and their location within transposon Tn4401, and the blaKPC product was sequenced to identify the KPC allele. Genotypic analysis was performed using repeated extragenic palindromic PCR (rep-PCR) and Multi Locus Sequence Typing (MLST). Finally, a conjugation assay determined whether the blaKPC genes were carried on transferable plasmids. The results indicate that the 32 isolates contained the blaKPC-2 variant associated with isoform Tn4401b, and were distributed in nine sequence types (ST), one of which was new. Conjugation assays indicate that 87.5% of the isolates contain the gene blaKPC on mobilizable plasmids. In these hospitals, the blaKPC-2 gene is spreading through the plasmids carrying the transposon Tn4401b. The most common ST belongs to Clonal Complexes CC258 and CC147, which play an important role in the dispersion of resistance to carbapenems worldwide.

Published
2017

Catalog

Books, media, physical & digital resources
See catalog results