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3. Specific IgE density assay: A new reverse enzyme allergosorbent test-based procedure for the quantitative detection of allergen-specific IgE

Author

Paolo Falagiani, Gianni Mistrello, Giuseppina Rapisarda, Graziella Riva, Daniela Roncarolo, Dario Zanoni, Susanna Voltolini, and Emanuele Crimi

Subjects
allergens, IgE density, radioallergosorbent test, reverse enzyme allergosorbent test, specific IgE, total IgE, Immunologic diseases. Allergy, RC581-607
Abstract

A new method is described for the quantitative detection of IgE antibodies, based on IgE capture with a specific antibody, reaction with liquid-biotinylated allergens and biotinylated anti-IgE and immunoenzymatic development of the reaction (reverse enzyme allergosorbent test). Using a reference system based on the World Health Organization IgE International standard, this method determines total IgE in the range 2-100kU/L and specific IgE in the range 0.2-100 kU/L, from which the specific/total ratio, called 'specific IgE density', can be calculated. This procedure has been applied to the study of specific IgE in 23 sera from patients polysensitized to pollen and mite allergens: 11 with asthma and 12 with rhinitis. The sensitivity and reproducibility of the method were evaluted. Sera from asthmatic patients showed higher cumulative levels of specific IgE (mean density 57.7%) than sera from rhinitic patients (mean density 32.6%). The clinical significance of specific IgE density in patients with multiple sensitizations is discussed.

Published
1999
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18. Allergy to fishing bait

Author

Tripodi, S., Falagiani, P., Perinelli, T., DellʼOmo, F., and Cristaldi, A.

Published
2002

20. Sublingual swallow or spit?

Author

Passalacqua, G., Villa, G., Altrinetti, V., Falagiani, P., Canonica, G. W., Mariani, G., and Bagnasco, M.

Published
2001

22. Food allergy to frog

Author

Romano, C., Ferrara, A. M., Cislaghi, C., and Falagiani, P.

Published
2000

27. A new apple extract

Author

Asero, R., Mistrello, G., Roncarolo, D., Antoniotti, P., Cislaghi, C., and Falagiani, P.

Published
1999

48. Systemic nickel allergy syndrome (SNAS): A review

Author

FALAGIANI P., GIOACCHINO M. D., RICCIARDI L., MINCIULLO P. L., SAITTA S., CARNÍ A., SANTORO G., GANGEMI S., MINELLI M., MAURO S., SCHIAVINO D., BOZZETTI, Maria Giuseppina, MASSARI, SERAFINA, Falagiani, P., Gioacchino, M. D., Ricciardi, L., Minciullo, P. L., Saitta, S., Carní, A., Santoro, G., Gangemi, S., Minelli, M., Bozzetti, Maria Giuseppina, Massari, Serafina, Mauro, S., and Schiavino, D.

Subjects
systemic syndrome, nickel allergy, SNAS
Published
2008

49. Lymphoproliferative Disease in Human Peripheral Blood Mononuclear Cell-Injected SCID Mice. IV. Differential Activation of Human Th1 and Th2 Lymphocytes and Influence of the Atopic Status on Lymphoma Development

Author

Vincenzo Coppola, Veronesi, A., Indraccolo, S., Calderazzo, F., Mion, M., Minuzzo, S., Esposito, G., Mauro, D., Silvestri, B., Gallo, P., Falagiani, P., Amadori, A., and Chieco-Bianchi, L.

Subjects
Adult, Hypersensitivity, Immediate, Male, Mice, Inbred BALB C, Lymphoma, Immunology, Mice, SCID, Immunoglobulin E, Th1 Cells, Flow Cytometry, Lymphocyte Activation, Interferon-gamma, Mice, Th2 Cells, Antigens, Heterophile, Immunoglobulin G, Leukocytes, Mononuclear, Animals, Humans, Immunology and Allergy, Female, Interleukin-4, RNA, Messenger, Injections, Intraperitoneal
Abstract

Intraperitoneal transfer of PBMC from EBV+ donors into SCID mice leads to high human Ig levels in mouse serum and B cell lymphoproliferative disease. As these events depend on the activation of coinjected human T cells, we addressed the behavior of the Th1 and Th2 subsets in this model. Production of IFN-γ, but not of Th2 cytokines such as IL-4, was detected in culture supernatants of PBMC stimulated in vitro with mouse splenocytes. Moreover, anti-CD3 stimulation of the human cells recovered from mice brought about IFN-γ, but not IL-4, synthesis; on the other hand, PCR and in situ hybridization analysis of ex vivo-recovered cells disclosed the presence of mRNA for both cytokines following in vitro restimulation, thus suggesting post-transcriptional regulation of IL-4 gene expression. When SCID mice were inoculated with PBMC from atopic donors, whose Th1/Th2 profile displays an imbalance toward Th2 cells, tumor development rates were lower, and tumor latency was higher, compared with those in mice injected with PBMC from normal donors. Isotypic analysis of human Ig in mouse serum showed the exclusive presence of IFN-γ-driven IgG subclasses; in addition, human IgE were low or undetectable in most cases. These findings indicate that following transfer into SCID mice, human Th1 lymphocytes undergo preferential activation, whereas Th2 function is down-regulated. Th1 lymphocytes probably are a major component in promoting EBV+ B cell expansion and tumor development; the individual Th1/Th2 profile could in part account for the as yet unexplained donor variability in tumor generation in this experimental model.

Published
1998
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