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Diabetes Mellitus (DM) is a major metabolic disorder causing morbidity and mortality worldwide. The prominent adverse effects of allopathic drugs are the principal reason for an escalating number of people seeking alternative and complementary-based therapies that may have less or no adverse effects. The poly-herbal formulation Vilva Kudineer Choornam (VKC) is a combination of Siddha medicinal plants for the treatment of diabetes. The present study aimed to investigate the antioxidant and antidiabetic efficacy of poly-herbal formulation by invitro assays. The Aqueous Extract of Formulation (AEF) and Ethanol Extract of Formulation (EEF) were used for the assay. In the present research work, the antioxidant potential of polyherbal formulation (VKC) was evaluated using DPPH radical scavenging and Nitric oxide radical scavenging assay. The IC50 value of AEF was 333.02 ?g/ml and EEF was 86.37 ?g/ml in DPPH radical scavenging assay whereas, in nitric oxide scavenging assay, the IC50 of AEF and EEF were found to be 8.61 ?g/ml and 447.77 ?g/ml, respectively. The VKC was also screened for in-vitro antidiabetic potential by alpha-amylase inhibition assay and it was shown to have significant percentage inhibition of alpha-amylase. The IC50 of EEF and AEF were found to be 868.84 ?g/ ml and 247.09 ?g/ml, respectively. The findings suggest that the polyherbal formulation (VKC) has significant antioxidant and antidiabetic activity. Further in vitro studies using cell lines and other enzymes may be carried out to fully establish the anti-diabetic potential of the polyherbal formulation.

Bacterial meningitis is considered as neurologic emergency with high morbidity and mortality rates. Mortality can reach 34%, especially in infections caused by S. pneumoniae and L. meningitides, while morbidity in bacterial meningitis patients, namely long-term neurologic sequelae, can reach 50% amongst survivors. If antibiotics are used properly, they can lower mortality rates.On the other hand, the irrational use of antibiotic therapy will raise the likelihood of resistance, which raises morbidity, mortality, and costs for health care. This study aims to determine the quality of antibiotic use in bacterial meningitis patients using the Gyssens method. It is an observational study employing the retrospective cross-sectional method conducted at Fatmawati General Hospital, Jakarta. The research subjects were 24 patients with bacterial meningitis who met the inclusion criteria. In this study, 45.8% of the subjects had been administered appropriate antibiotics and 54.2% inappropriate ones, which were spread across several categories, namely category IVc for one subjects (4.2%); category IIIA for two subjects (8.3%); category IIIB for one subject (4.2%); category IIA for ten subjects (41.7%); and category IIB for two subjects (8.3%). The use of appropriate antibiotics based on evaluation using the Gyssens algorithm did not significantly affect patient outcomes (p=0.542). The independent variables (type of antibiotic therapy, number of antibiotics, duration of antibiotic use) and confounding ones (age, gender, number of comorbidities, type of comorbidities, length of treatment) also had no significant effect on the quality of antibiotic use, with a p value more than 0.05. Abstrak: Meningitis bakterial dianggap sebagai kasus kegawatdaruratan neurologik dengan angka morbiditas dan mortalitas yang tinggi. Mortalitas akibat meningitis bakterial dapat mencapai 34% terutama pada infeksi yang disebabkan oleh S. pneumoniae dan L. meningitidis. Sementara morbiditas pada pasien m

The consumption of tropical fruits rich in chemopreventive compounds are required to prevent cells carcinogenesis and proliferation. This study was designed to assess de biocompatibility of Detarium microcarpum fruit extract on normal fibroblasts and its antiproliferative potentiality on human osteosarcoma MG-63 cells. Primary dermal fibroblasts and human osteosarcoma MG-63 cells were treated with different concentrations of hexane, chloroform, ethyl acetate and methanol extracts of D. microcarpum fruit pulp for 24h, 48h and 72h. The biocompatibility property of extracts on the normal fibroblasts and its antiproliferative activity on the human osteosarcoma cells were evaluated by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenytetrazolium bromide (MTT) assay. The biocompatibility study of D. microcarpum fruit pulp showed that the chloroform extract has exhibited the highest cytotoxic effect on normal fibroblasts followed by the ethyl acetate extract. Hexane extract wasn’t cytotoxic at concentrations of 125 and 250 µg/mL but caused more than 80 % of cell death at a concentration of 500 µg/mL. Methanol extract didn’t show a significant cytotoxic effect. Furthermore, chloroform and ethyl acetate extracts showed the best antiproliferative activity on osteosarcoma cells. A complete cell death was observed when osteosarcoma cells were treated with ethyl acetate extract at all concentrations while chloroform extract at concentrations of 250 and 500 µg/mL caused a complete cells death. Methanol extract exhibited any antiprolifarative activity. Chloroform and ethyl acetate extract of fruits pulp of D. microcapum are potent source of anticancer phytomolecules and have potential to be a promising anti-osteosarcoma extract.

The study evaluated the antioxidant and hypoglycaemic potentials of the ethanol flower extract of Bougainvillea spectabilis. Flower extract of Bougainvillea spectabilis were obtained by cold maceration method. The preliminary phytochemical screening and hypoglycaemic effect were carried out using standard methods. Antioxidant screening was carried out using DPPH antioxidant assay test. Five rats were used for each group; normal control, standard control, 100, 200 and 400 mg/kg b.w. doses of the crude ethanol flower extract of B. spectabilis to test the hypoglycaemic effect in alloxan-induced diabetic rats. Blood glucose levels were measured at different time intervals. The preliminary phytochemical screening revealed the presence of alkaloids, saponins, strerols, terpenoids, flavonoids, and carbohydrates. The crude ethanol fraction demonstrated least antioxidant activity while the chloroform fraction showed the highest antioxidant activity followed by the hexane fraction. The ethanol flower extract showed a significant dose dependent hypoglyceamic effect after 24 hours of administration. Of the doses tested, highest hypoglycaemic effect was observed by the ethanol flower extract of the dose 400 mg/kg at 24 hours. The findings revealed that non-polar fractions have more antioxidant activity than the polar fractions while the ethanol flower extract of Bougainvillea spectabilis possesses a delayed hypoglycaemic effect.

Lung cancer is the leading cause of cancer deaths worldwide, and this makes it an attractive disease to review and possibly improve therapeutic treatment options. he extreme lethality of lung cancer is ascribed to the lack of early diagnostic strategies as in almost 50 % of the cases the disease is confirmed in stage IV, leaving low chance of survival. The inaccessibility to the deeper portions of the lung for conventional therapy further adds up to the complication in the treatment process. Surgery, radiation, chemotherapy, targeted treatments, and immunotherapy separate or in combination are commonly used to treat lung cancer. However, these treatment types may cause different side effects, and chemotherapy-based regimens appear to have reached a therapeutic plateau. Hence, effective, better- tolerated treatments are needed to address and hopefully overcome this conundrum. Nanocarriers through inhalational route offer many advantages like; 1) they achieve uniform distribution of drug among the alveoli, 2) better solubilization of the drug, 3) sustained drug release which subsequently decreases dosing frequency, 4) better patient compliance, 5) lesser side effects, and 6) improved drug internalization to the cells .Therefore, targeted inhalable NP delivery to the lungs is a potential area of research in cancer nanotechnology that catches the attention of many formulation scientists, oncologists, and biomedical researchers. Based on this literature review, we will discuss the development, characterization, and benefits of inhalable nanocarriers for local treatment of lung carcinoma.

Context: Schistosomiasis continues to be the leading cause of morbidity and mortality among the neglected tropical diseases. Apart from the high cost of chemotherapy, concerns over drug resistance and tolerance have been raised in the past decade. Objective: The aim of the study was to evaluate the anticercarial activity of extracts and compounds from the stem bark of Holarrhena floribunda on cercaria of Schistosoma haematobium. Methods: Hydroethanolic and alkaloidal extracts from the stem bark of H. floribunda were tested on cercaria at concentrations between 500.00 and 15.625 μg/mL for 180 minutes and assessing the percentage viability at time intervals of 0, 15, 30, 60, 120 and 180 minutes. Praziquantel, used as reference drug, and the isolated compounds were tested at similar concentrations. The cercaria mortalities and IC50 of extracts and compounds were estimated after 30 minutes of incubation. Results: The 70 %v/v ethanol extract showed the highest activity (IC50=20.09±1.11 μg/mL) with praziquantel giving IC50 of 695.50±1.12. The alkaloids holonamine, holadienine and conessine, isolated from the stem bark, showed considerable cercaricidal activity with the latter recording an IC50 of 33.28±1.04. Conclusion: The study gives first-hand knowledge of the anti-cercarial activity of H. floribunda and its steroidal alkaloids. This gives credence to the traditional uses of the plant as an anti-parasitic agent.

Bougainvillea spectabilis Willd. is an ornamental plant cultivated in tropical, subtropical regions and other places as Egypt. The present study aimed to perform bioassay guided fractionation and isolation of some of the bioactive compounds from the Egyptian cultivate. The total ethanol extracts of the leaves (T.ET.L.), stems (T.ET.S.) and flowers (T.ET.F.) were screened for some pharmacological activities viz. in vivo anti-oxidant and anti-hepatotoxic, in addition to in vitro cytotoxic activities. The anti-oxidant effect was assessed by measuring serum glutathione level (GSH) in alloxan-induced diabetic rats. The anti-hepatotoxic activity was evaluated via measuring serum markers level viz. alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) in CCl4-induced hepatotoxicity in rats. In vitro cytotoxicity of the different extracts was estimated for liver cancer cell line (HEPG2) adopting Sulforhodamine B stain assay. T.ET.L. exhibited significantly potent anti-oxidant and anti-hepatotoxic activities, while T.ET.S. showed the highest cytotoxic activity. Through biological guided fractionation, leaves and stems were subjected to successive solvent extraction, whereas the leaves ethyl acetate (Et.Ac.L.) and the stems ethanol 70% (Et.70%S.) extracts showed highly potent activities. Thus, different chromatographic techniques were performed on Et.Ac.L. and Et.70%S. extracts leading to the isolation of five bioactive metabolites. Three flavonoids were isolated from Et.Ac.L.; genistein-7-O-rutinoside (1), formononetin-7-O-rutinoside (2) and myricetin (3), while orobol-7-O-glucoside (4) and hesperidin (5) were isolated from Et.70%S. This work demonstrated the importance of the plant as a promising anti-oxidant, anti-hepatotoxic and cytotoxic product for nutraceutical use.

In this paper the impact of air pollution on the bronchial reactivity of mine workers in Leposaviq - Kosovo with toxic metals such as: (SiO2, Pb, Zn, Particulate matter-PM) has been studied. Since body plethysmography is a highly sensitive and precise technique in the research of phenomena affecting bronchial reactivity, we have defined lung function with Body Plethysmography. Airway resistance (Raw) was recorded and Intrathoracic Gas Volume (ITGV) was measured and Specific Airway Resistance (SRaw) and specific conductance (SGaw) were calculated. The control group consisted of 35 healthy people. In our case we used histamine as a provocative test, which did not cause significant changes in bronchomotor tone (p> 0.1). In the experimental group, 59 workers of the Leposaviq - Kosovo mine were included. The results obtained from this research show that the mean Specific Resistance (SRaw), and specific conductance (SGaw) values significantly underwent changes in the experimental group compared to the control group (p <0.01). The study also found that smoking favors the adverse effects of air pollution on workers’ health. To the workers exposed to high concentrations of air pollution found change in lung function, increased bronchial reactivity, and impaired lung function at the level of gas exchange, namely a decrease in O2 partial pressure and an increase in pacific CO2 pressure in arterial blood. We also have a reduction in carbon monoxide transfer factor (p <0.01).

Background: Phytochemicals are present as important substances in natural resources and therefore, have aided in invention of the anti-oxidant, and anti-elastase properties of polyphenol compounds present in Indonesian herbs such as Macassar Kernels (Rhus javanica L). Objective: This research aimed to investigate anti-elastase, and antioxidant properties. Methods: Ethanolic extract of Rhus Leaves (RL), Rhus Stem (RS), Rhus Greenish Fruit (RGF), and Rhus Blackish-grey Fruit (RBF) were prepared individually by solvent extraction method. Anti-elastase activity was carried out with elastase from porcine pancreas. For quantitative phytochemical screening, DPPH radical scavenging assay, Ferric Reducing Antioxidant Power Assay (FRAP), Total Phenolic Content (TPC), Total Flavonoid Content (TFC) of Rhus javanica were estimated. Result: RS showed highest anti-elastase activity (45.30%±0.087), compared with RL (12.30%±0.004), and there were no activities in RGF and RBF. In DPPH assay, RS had lowest activity (IC50 561.05 μg/ml), compared with RBF (IC50 239.28 μg /ml), RGF (IC50 189.3I μg/ml), and RL (IC50 157.81 μg/ml). RS also has lowest FRAP activity (% inhibition = 26.60%±0.002), and TPC value (28.50±0.03 mgGAE/g dry weight). Conclusion: Test extracts showed anti-aging properties in different mechanisms. RS possessed the highest anti-elastase activity but had the weakest antioxidant activity.

Objective: The most common type of phytochemical is phenolic compounds. These compounds are mostly found in the plant kingdom, and extraction is one of the first and most important stages before any use. The green chemistry trend has encouraged the researcher to discover and generate innovative solvents in the extraction of phenolic compound (PC) as a strategy to toxic and potentially hazardous organic solvents. Natural Deep Eutectic Solvents (NADES) have recently been used as phenolic compound extraction solvents. The objective of this study is to determine the total phenolic contents of the NADES extract from comfrey (Symphytum officinale). Methods: This study used choline chloride-urea, choline chloride-glycerol and choline chloride-sucrose combination of NADES for extraction of phenolic compound in comfrey. The extraction was helped using Ultrasound Assisted Extraction (UAE). The extracted material was examined using a UV-Vis spectrophotometer. Results: The results showed that the total phenol of choline chloride-urea, chloride-glycerol and choline chloride-sucrose NADES comfrey extraction was 2.069, 1.566, and 1.906 mg GAE /gram of dry powder, respectively. Conclusion: Based on the result, it can be concluded type of NADES combination showed a different level of phenolic extraction. The best phenolic extraction was shown in the combination of choline chloride-urea. Abstrak: Latar belakang: Fenol adalah senyawa kimia yang paling umum ditemukan dalam tanaman. Proses ekstraksi berperan penting dalam pemisahan senyawa fenol dari tanaman sebelum dimanfaatkan. Saat ini penelitian banyak difokuskan pada proses ekstraksi yang ramah lingkungan. Salah satunya adalah menggunakan Natural Deep Eutectic Solvent (NADES) dalam proses ekstraksi senyawa fenol. Tujuan penelitian ini adalah menghitung kadar fenol total dalam ekstrak NADES tanaman komfrey. Metode: Penelitian ini menggunakan NADES yang berasal dari kolin klorida-gliserol, kolin klorida-urea dan kolin klorida-sukrosa

Alpha-1 antitrypsin (AAT) is a serine protease inhibitor. A low level of serum AAT (<11 µM) is defined as AAT deficiency. The limitations of the current treatments for AAT deficiency-related lung disease are the low AAT lung penetration rate following intravenous infusion and the rapid AAT clearance from the lung following inhalation. Here, we present a PEGylated version of AAT, which shows improved resistance to proteolysis in vitro. In vivo, PEGylated AAT exhibits prolonged body residency after delivery by the intravenous and intratracheal routes. In a murine COPD model, a low intranasal dose of PEGylated AAT achieves better efficacy than a 45-fold higher intravenous dose of AAT. PEGylated AAT represents a promising alternative for AAT augmentation therapy., (BIFA - Sciences biomédicales et pharmaceutiques) -- UCL, 2022

Vagus nerve stimulation (VNS) is an established treatment for drug resistant epilepsy (DRE), which affects about 30% of epilepsy patients. On-demand triggering of VNS is known to improve its efficacy in DRE. Automatic on-demand VNS needs an effective and reliable seizure detection method. As seizures are commonly associated with autonomic manifestations and the vagus nerve carries parasympathetic and sensory information from and to the viscera we hypnotize that seizure biomarker can be recorded from vagus nerve electroneurogram (VENG) recording. In this thesis we established a method to reliably record VENG signals in anesthetized animals during acute pentylenetetrazol induced seizures and confirmed the neural origin of those signals. Furthermore, we confirmed the existence of seizure related signals in the VENG recording and developed a seizure detection method based on them., (BIFA - Sciences biomédicales et pharmaceutiques) -- UCL, 2022

Oil and its derivatives spills have been a major issue across decades due to transport, import, Export and accidents with its wider use in industry and it is difficult to be biodegraded. Many techniques were developed to clean up petroleum contaminated soil, among all of them, the biological treatments are more efficient and economic compared to chemical and physical ones. Bioremediation is applied as a treatment technology that is cost-effective, ecologically friendly and efficient for the decontamination of hydrocarbon pollution. In this study, crude oil contaminated soil samples were collected from oil extraction fields in Libya. The bacterial strains were isolated using selective media (agar containing crude oil). Isolated bacteria were identified using microbial features and selective media and then used in biodegradation experiment of 0.5% and 1% of crude oil contaminated soil. The bacterial concentration was adjusted to 1.5 x 108 scf/ml before supplementing in the soil. Three plants were chosen to perform experiment (Malva punilora, Ricinus communis and Triticum repens) on 0.5% and 1% crude oil contaminated soil. The chosen species were implanted directly in the contaminated soil together with prepared bacterial consortia. Soil sample (triplicate) was taken from each experiment at zero time, after 15 days, and 30 days of experiment, hexane was added to the soil samples and the absorbance was measured using spectrophotometer at 360 nm. As a result, two bacterial strains proved to be oil biodegrades were isolated and identified as Escherichia coli and other bacillus bacteria. The percentage of crude oil removal by bacteria was more than 60% in the soil contaminated by 0.5% of crude oil after 15 and 30 days of experiment, while it was 30% at 1% crude oil contaminated soil after 15 days and increased to be 75% after 30 days of experiment. The experiment included combination between plants and bacteria resulted in the 90% of removal by Triticum repens and bacteria

Cystic fibrosis, CF, is one of the most common life-shortening genetic diseases among Caucasian population. It is a recessive genetic disease that is mainly caused by different types of mutations affecting the gene encoding for the cystic fibrosis transmembrane conductance regulator, CFTR, protein. CFTR is a multifunctional protein found in multiple organs in the human body, acting mainly as a trans-epithelial channel which regulates the flow of chloride and bicarbonate ions across epithelial membranes leading to the formation of a thin slippery mucous layer. A malfunctioning CFTR protein would lead to the accumulation of a thick viscous mucous layer blocking pancreatic ducts, intestines and airways which is the primary reason of death. Treatment of cystic fibrosis was mainly addressing the symptoms to overcome the complications of the disease such as pneumonia, lung infections, pancreatitis, maldigestion and infertility. Since the early 2010’s, the development of an actual therapy has reached great milestones including small molecule modulators and genetic therapy. Small molecule therapy depends on the development of small pharmacological agents that can bind to the mutated CFTR protein restoring its function. Pharmacological agents can act through different mechanisms and be mainly classified to; correctors and potentiators. On the other hand, gene-editing techniques are evolving showing very promising results. Gene therapy entails the relocation of a proper copy of the CFTR gene in the aim of expressing a functional CFTR protein. In this review article, small molecule and genetic therapies will be discussed including their development, benefits and limitations.

Hyperlipidemia is a condition of abnormally high lipids levels in the blood which has been ranked as one of the greatest risk factors contributing to prevalence and severity of coronary heart disease. The available antihyperlipidemic drugs have been associated with some side effects however, herbal management of hyperlipidemia are relatively safe, cheap and readily available. P. conophora is an edible plant consumed in Nigeria as snack and speculated to have beneficial effect on blood lipid profile. The present study evaluates anti-hyperlipidemic effect of aqueous extract of cooked P. conophora nut using in vivo and in vitro experimental models.The anti-hyperlipidemic activity was evaluated using tyloxapol induced-hyperlipidemic rats by intraperitoneal injections of Tyloxapol at a dose of 300 mg/kg body weight and high cholesterol-diet induced rats by oral administration of high cholesterol diet for 60 days. Cholesterol esterase enzyme inhibition was used for the in vitro evaluation.Aqueous extract of P. conophora at varying doses, reduced the elevated lipid parameters in both models; the dose of 500 mg/kg showed comparable hypolipidemic effects with standard drug (Simvastatin) at 10 mg/kg (P<0.01). The extract also inhibited cholesterol esterase enzyme with IC50 value of 129.30±0.10μg/ml while Simvastatin with IC50 value of 51.42±0.13μg/ml. Preliminary phytochemical analysis revealed the presence of; Flavonoids, saponin, cardiac glycoside, alkaloids, tannins, steroids and reducing sugar.P. conophora extract exhibited strong hypolipidemic activity and the dose of 500mg/kg demonstrated equipotent activity as the standard drug; Simvastatin 10mg/kg. The extract also showed inhibitory activity against pancreatic cholesterol esterase enzyme; hence can be used to limit absorption of dietary cholesterol, prevent and treat hyperlipidemia.

Evidence based use of herbal medicines has a positive implication for both users and society. In this study, component plant materials of a traditional Ghanaian polyherbal skin product comprising Eugenia caryophyllata, Zanthoxylum zanthoxyloides Tridax procumbens, Psidium guajava and Alchornea cordifolia and used in the management of superficial skin infections was evaluated to establish their contribution to the overall therapeutic activity of the product. Each of the five (5) plants was subjected to an in vitro antimicrobial assay using the microtitre broth technique followed by an interactive combination assay for plants demonstrating noteworthy antimicrobial activity (MIC ≤ 1.0 mg/ml). Test strains included Staphyloccocus aureus, Candida albicans, Trichophyton rubrum, Epidermophyton floccusum and Microsporum canis. Eugenia caryophyllata, Zanthoxylum zanthoxyloides and Alchornea cordifolia showed better activity than Psidium guajava, Tridax procumbens and the Total Crude Extract (combination of the 5 extracts). The binary combination of Eugenia caryophyllata and Alchornea cordifolia indicated synergistic and additive activity against all the test strains. An improved biological activity was also observed when a mixture of the two (2) plants at a ratio of Eugenia caryophyllata 60 % (w/w) and Alchornea cordifolia 40 % (w/w) was assayed. A follow up pilot clinical study established that this new recipe was clinically effective but of lower therapeutic effect compared to the original product. In conclusion, the original formulation of the product may be preferred because of the shorter duration of treatment which reduces the risk of harms and cost of treatment.

Acridone is a unique naturally occurring alkaloid known to associate with several biological activities. 2,3-dimethoxy-10-methyl-10,8a-dihydroacridin-9(8aH)-one (4) and its precursor 2-((3,4-dimethoxyphenyl)methylamino)benzoic acid (3) were synthesized and investigated for potential antioxidant and inhibitory activity against acetylcholinestrase. The synthetic pathway involves reaction of 2-(methylamino) benzoic acid (1) with 4-chloro-1,2-dimethoxybenzene (2) in presence of CuO and K2CO3 to give the precursor 3. Subsequent, cyclcondensation of 3 with Conc. H2SO4 afforded the anticipated acridone 4. Furthermore, the dimethoxyacridone derivative 4 showed potent antiacetylcholinesterase (ACHE) activity at (100 uM) with IC50 = 9.25 uM that is as potent as the reference drug rivastigmine. Assessment of total antioxidant activity of compounds 3 & 4 in comparison to known standard compounds revealed the following order: α-tocopherol > Acridone 4 > trolox > butylated hydroxyl anisole (BHA) > butylated hydroxyl toluene (BHT) > compound 3. Molecular docking characteristics of 3 & 4 within the active site of AChE (PDB: 1ACJ) co-crystallized with 9-amino-tetrahydroacridine (Tacrine) have been studied. Interestingly, the results revealed comparable binding poses to the co-crystallized ligand and demonstrates good correlation of the binding energy (DG) with the observed IC50-values. This finding suggests that compounds 3 & 4 exhibit good antioxidant effect and inhibition of acetylcholinesterase, which might provide profitable candidates in management of Alzheimer’s disease.

Background: It has been reported by the Ministry of Health that the prevalence of wounded and worsening events is increasing in Indonesia. This situation requires more intensive attention. Eel mucus has been an alternative treatment of wounds in some parts of Indonesian society since ancient times. Aim and Objectives: To explore the effectiveness of eel mucus ointment as a natural wound healing product. Material and Methods: This experimental study used 45 mice divided into 3 groups; 15 mice in each group. All mice were aseptically wounded 1.5 cm on their anterior region of back skin using a sterile scalpel, and then treated with placebo, povidone iodine ointment, and with eel mucous ointment (Belutidine). On the third, sixth and ninth day, the mice were sacrificed. Data on wound length were taken on 9th day. Microscopic data of histologic slides collected were: number of neutrophil, mono-nuclear, neo-vascular and fibrosis cells. Data were analyzed using statistical analysis. Results: There was a significant difference in wound length between the groups (p = 0.008). The number of mono-nuclear cells on the third day was different significantly among the groups (p = 0.008). There was no significant difference of the overall observed cells on day 6 and there was a difference in mean number of neutrophils and fibrosis on day nine (p < 0.05). On day nine, the group treated with eel mucus ointment had the highest number of mean of neo-vascular and fibrosis cells. Conclusion: Eel mucous ointment is effective for wound healing.