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Low quality and yield of methane severely hindered the industrial application of straw biogas fermentation and there has been no effective solution found so far. In this study, a novel method has been developed to improve the quality and yield of methane during biogas fermentation. We coupled microbial electrolysis cell (MEC) with normal anaerobic fermentation to enhance the yield and purity of methane. This novel fermentation process achieved the methane purity of more than 85%, which was critically higher than those of previously published reports. With the stimulation from microorganisms and electric current, the degradation of fiber has been greatly enhanced. The MEC system significantly improves the yield and purity of biogas, bringing a new path to synthesize the methane by the carbon dioxide and hydrogen ions in solution under the electron. Electrochemical index analysis showed the extra methane synthesis which was mainly led by the external circuit electron transfer. The result of the gas chromatograph and solid degradation rate showed that the carbon source of extra methane was CO2 produced in normal fermentation and extra volatile solid degradation. These results show that MEC significantly enhanced the quality and yield of methane in a straw fermentation process, giving insights for normal anaerobic fermentation.

Levoglucosan is a promising sugar present in the lignocellulose pyrolysis bio-oil, which is a renewable and environment-friendly source for various value-added productions. Although many microbial catalysts have been engineered to produce biofuels and chemicals from levoglucosan, the demerits that these biocatalysts can only utilize pure levoglucosan while inhibited by the inhibitors co-existing with levoglucosan in the bio-oil have greatly limited the industrial-scale application of these biocatalysts in lignocellulose biorefinery. In this study, the previously engineered Escherichia coli LGE2 was evolved for enhanced inhibitor tolerance using long-term adaptive evolution under the stress of multiple inhibitors and finally, a stable mutant E. coli-H was obtained after ~ 374 generations’ evolution. In the bio-oil media with an extremely acidic pH of 3.1, E. coli-H with high inhibitor tolerance exhibited remarkable levoglucosan consumption and ethanol production abilities comparable to the control, while the growth of the non-evolved strain was completely blocked even when the pH was adjusted to 7.0. Finally, 8.4 g/L ethanol was achieved by E. coli-H in the undetoxified bio-oil media with ~ 2.0% (w/v) levoglucosan, reaching 82% of the theoretical yield. Whole-genome re-sequencing to monitor the acquisition of mutations identified 4 new mutations within the globally regulatory genes rssB, yqhA, and basR, and the − 10 box of the putative promoter of yqhD-dgkA operon. Especially, yqhA was the first time to be revealed as a gene responsible for inhibitor tolerance. The mutations were all responsible for improved fitness, while basR mutation greatly contributed to the fitness improvement of E. coli-H. This study, for the first time, generated an inhibitor-tolerant levoglucosan-utilizing strain that could produce cost-effective bioethanol from the toxic bio-oil without detoxification process, and provided important experimental evidence and valuable genetic/proteinic information for the development of other robust microbial platforms involved in lignocellulose biorefining processes.

Although the process of microbial degradation of coal to produce biomethane has got much attention from many research works, the products profiles of coal at the initial stage of methanogenic bioconversion are not clear yet. In this study, five coal‐degrading bacterial strains (CD1, CD10, CD20, CD24, and CD25) from a methanogenic community were isolated and identified. Among them, CD1 and CD24 belong to Paenibacillus sp., CD10 and CD20 belong to Bacillus sp., and CD25 belongs to Stenotrophomonas sp. After biotreatment of lignite and bituminous coal, the kinds of newly produced compounds were 33 and 45, respectively. Metabolomics analysis showed that a large number of alkane compounds and heterocyclic aromatic compounds were produced after degradation of bituminous coal and lignite by isolated bacteria, and most of the compounds had been produced in a hydroxylated or acylated manner, indicating that the initial microbial treatment enhanced the bioavailability of coal. Some alkaloids and biosurfactants were also detected in the aforementioned products, such as glycerophosphocholine, proveratrol A, proveratrol B, surfactin, etc. These microbial metabolites may play an important role in solubilization during the degradation of coal. This study added to the understanding of the complicated metabolic process of methanogenic coal bioconversion and enabled effective production of biomethane with appropriate metabolic strategies.

Ageing is characterized by the maintaining deterioration of homeostatic processes over time, leading to functional decline and increased risk of disease and death. Several distinct mechanisms underlying ageing have been reported and mounting shreds of evidence have shown that histone methylation, an epigenetic marker, regulates gene expression during ageing. Recently, SET-domain genes have gained attentions and have been identified as histone methyltransferase involved in ageing process. Deletion of these genes extends lifespan and increased oxidative stress resistance in Caenorhabditis elegans depends on the daf-16 activity in the insulin/IGF pathway. In this chapter, we propose to investigate the role of histone methylation in the process of ageing and oxidative stress with an emphasis on the role of set-18 gene in ageing process.

Currently, researchers have focused on electrokinetic (EK) bioremediation due to its potential to remove a wide-range of pollutants. Further, to improve their performance, synthetic surfactants are employed as effective additives because of their excellent solubility and mobility. Synthetic surfactants have an excessive position in industries since they are well-established, cheap, and easily available. Nevertheless, these surfactants have adverse environmental effects and could be detrimental to aquatic and terrestrial life. Owing to social and environmental awareness, there is a rising demand for bio-based surfactants in the global market, from environmental sustainability to public health, because of their excellent surface and interfacial activity, higher and stable emulsifying property, biodegradability, non- or low toxicity, better selectivity and specificity at extreme environmental conditions. Unfortunately, challenges to biosurfactants, like expensive raw materials, low yields, and purification processes, hinder their applicability to large-scale. To date, extensive research has already been conducted for production scale-up using multidisciplinary approaches. However, it is still essential to research and develop high-yielding bacteria for bioproduction through traditional and biotechnological advances to reduce production costs. Herein, this review evaluates the recent progress made on microbial-surfactants for bioproduction scale-up and provides detailed information on traditional and advanced genetic engineering approaches for cost-effective bioproduction. Furthermore, this study emphasized the role of electrokinetic (EK) bioremediation and discussed the application of BioS-mediated EK for various pollutants remediation.

The slow reduction of Fe(III) to Fe(II) in electro-Fenton technology limits pollutant removal and causes Fe sludge production. This study hypothesizes that Shewanella, a dissimilatory iron-reducing...

Gastrointestinal (GI) microbiota is one of the most complicated microbial ecosystems and is vital in regulating biological processes associated with nutrient absorption and homeostatic maintenance. Although several efforts have been achieved in characterizing bacterial communities across gut regions, the variation of non-bacterial communities across GI tracts is still largely unexplored. To address this, we investigated microbial biogeography throughout the whole GI tracts of Ujimqin sheep (Ovis aries) by amplicon sequencing which targeted bacteria, fungi, and archaea. The results indicated that the community structures of all three domains were significantly distinguished according to GI tracts (stomach, small intestine, and large intestine), and a more strong and efficient species interaction was detected in small intestine based on cross-domain network analysis. Moreover, a between-domain difference in microbial assembly mechanism of among-GI regions was revealed here, wherein bacterial community is dominantly governed by variable selection (explaining ~62% of taxa turnover), while fungal and archaeal communities mainly governed by homogenizing dispersal (explaining ~49% and 60% of the turnover, respectively). Overall, these data highlight the GI section- and domain-dependence of GI microbial structure and assembly mechanism, suggesting that multi-domain should be explicitly considered when evaluating the influences of GI selection on gut microbial communities. This article is protected by copyright. All rights reserved.

This study has investigated the key contribution of sodium acetate as co-substrate on the performances of microbial fuel cells (MFC) during the conversion of a toxic blue-green algae (BGA) biomass into bioelectricity. Microcystis aeruginosa biomass was employed as a target substrate and sodium acetate (AC) as a co-substrate. Compared with MFC treated with BGA alone (MFC-BGA), the MFCs treated with BGA together with acetate (MFC-BGA-AC) significantly magnified the voltage output by 261.3%. Moreover, the addition of co-substrate extended the electric batch cycle by 253.1% and 7.9% compared with MFC-BGA-AC and its corresponding MFC treated only with AC (MFC-AC), respectively. The co-substrate strategy also enhanced the maximum power density by a factor of 3.53 MFC-BGA and 1.2 MFC-AC. It has also displayed the smallest charge transfer resistance of 12.01 Ω which was approximately 45.8% lower than that of MFC-BGA and even slightly lower than the control. Based on the transmission electron microscope results, the cell morphology was also less affected in MFC-BGA-AC. Thus, our new study revealed that the co-substrate could empower the bio-electrochemical active bacteria for toxin survival and make a crucial contribution in alleviating the internal resistance within the operating reactors, which consequently promote the transfer of the generated charges to the bio-anode for power production evolution. The current investigation will conspicuously help in paving ways toward sustainable clean energy production and durable wastes control in the aquatic environment.

A co-substrate strategy has the potential to contribute toward minimizing the poisoning of refractories on the anode respiring bacteria (ARB), which is a necessity for microbial fuel cell (MFC) to recover energy from toxic wastewater. However, little is known about the underlying mechanisms. This study employed 4-chlorophenol (4-CP) as target refractory pollutant and acetate as co-substrate. The co-substrate could stimulate 4-CP sensitive extracellular electron transfer (EET) enzymes’ activity under 4-CP stress, such as succinate dehydrogenase (complex II) and cytochromes c OmcA and OmcB. For the microbial community, ARB were still abundant during 4-CP toxification; Azospirillum and Dechloromonas were enriched to conduct aromatic ring breakage and dechlorination. Thus, a 4.3-fold increased power generation was achieved, and the 4-CP and chemical oxygen demand (COD) removals increased by ∼42% and ∼53%, respectively. An easy-to-degrade substrate could improve power re-coverage from refractory wastewater by enhancing EET enzyme activity and optimizing the microbial community.

In this study, microalgal biomass waste (Chlorella regularis) was treated while simultaneously producing bioelectricity in a microbial fuel cell (MFC). Algal biomass was the sole electron donor and was enriched with easily biodegradable proteins (46%) and carbohydrates (22%). The generated power density was 0.86 W/m2 and the columbic efficiency reached ∼61.5%.The power generation could be further increased to 1.07 W/m2 by using a biomass waste concentration enhancement strategy with maximum chemical oxygen demand (COD) removal of ∼65.2%. Via direct comparison, the power generation and COD removal capability of the algal-fed MFC was close to that of the commercial acetate-fed MFC. The algae-fed MFC presented superior electrochemical characteristics that were attributed to the complicated composition of the biomass anolyte. It possessed a multiple anode respiring bacterial group and diverse microbial community. Hence, this study provides a new strategy for the utilization of microalgal biomass as a bioresource.

The recharge of reclaimed water is an effective strategy for addressing the issues of water quality deterioration and groundwater level decline simultaneously. Residual Al coagulants are normally remained in the recovered water at low concentrations, and may induce clogging problems during the recharging process. However, this issue has been ignored in the past. In this study, we investigated the mechanisms of Al(III)-induced aquifer bio-clogging, the role of Al(III) in quartz sand media (SiO2) dissolution and re-precipitation in the series of aquifer columns. We determined that Al(III) resulted in serious clogging in ∼140 h at low concentrations that satisfied the national drinking water standard of China. The corresponding hydraulic conductivity decreased by more than ∼90% in the bacteria-containing aquifer, which was ∼30% greater than that for the bacteria-free trials. The enhanced Al(III)-related clogging was caused by modifying quartz sand to form Si-O-Al(OH)n and improving microbes attachment. Microbes retention kinetic coefficients (k) of the Al recharged simulated aquifer could increase by 3.0–8.3 times. The Al(III) also enhanced biomass production and clogging by binding to microbial extracellular polymeric substances. In turn, the greater amount of biomass accelerated the Si dissolution and re-precipitation, this may potentially damage the stability of aquifer structure. The results showed that reclaimed water treated with Al coagulation should be employed with caution for recharging.

Contamination caused by dense non-aqueous phase liquids (DNAPLs) is a serious peril to the groundwater supply quality. The DNAPLs are largely undetected and yet are likely to be a significant factor limiting site remediation. It is necessary to identify the sources of pollution and control pollution in the early stage. The primary objective of this research was to investigate the pollution source and intensity of migrating DNAPLs. This objective was accomplished through the combination of genetic algorithm (GA) and T2VOC numerical experimental simulation. This study considers two synthetic cases of numerical modeling based on the T2VOC from the TOUGH2 code family to assess and evaluate the efficacity of the algorithm. The first case identified concurrently the intensity as well as the location of the one unknown contaminated water source. The second case distinguished various contaminated water sources. For the single source pollution identification, the estimation of intensity error was 0.08%, and the error of GA at second and third true value calculation was 0.05% and 0.03%, respectively, achieving the goal of pollution identification accurately. While for the multiple sources, the deviation of intensity was ranging between 1.02 and 5.65%, the results suggested that source No.2 was mainly responsible for the leakage, and source No.3 adopted the secondary responsibility among others. These two cases suggested that GA established by optimal search strategy could be adopted for effective identification of DNAPL pollution sources. This study provides information that could facilitate the development and implementation of innovative remediation strategies.

Background Biosurfactants, being highly biodegradable, ecofriendly and multifunctional compounds have wide applications in various industrial sectors including environmental bioremediation. Surfactin, a member of lipopeptide family, which is considered as one of the most powerful biosurfactants due to its excellent emulsifying activities as well as environmental and therapeutic applications. Therefore, the aim of this study was to investigate the newly isolated bacterial strain S2MT for production of surfactin-like biosurfactants and their potential applications for oil-contaminated soil remediation. Results In this study, the strain S2MT was isolated from lake sediment and was identified as Bacillus nealsonii based on transmitted electron microscopy (TEM) and 16S rRNA ribo-typing. The strain S2MT produced biosurfactant that reduced the surface tension (34.15 ± 0.6 mN/m) and displayed excellent emulsifying potential for kerosene (55 ± 0.3%). Additionally, the maximum biosurfactant product yield of 1300 mg/L was achieved when the composition of the culture medium was optimized through response surface methodology (RSM). Results showed that 2% glycerol and 0.1% NH4NO3 were the best carbon/nitrogen substrates for biosurfactant production. The parameters such as temperature (30 °C), pH (8), agitation (100 rpm), NH4NO3 (0.1%) and NaCl (0.5%) displayed most significant contribution towards surface tension reduction that resulted in enhanced biosurfactant yield. Moreover, the extracted biosurfactants were found to be highly stable at environmental factors such as salinity, pH and temperature variations. The biosurfactants were characterized as cyclic lipopeptides relating to surfactin-like isoforms (C13–C15) using thin-layer chromatography (TLC), Ultra high performance liquid chromatography and mass spectrometry (UHPLC-MS). The crude biosurfactant product displayed up to 43.6 ± 0.08% and 46.7 ± 0.01% remediation of heavy engine-oil contaminated soil at 10 and 40 mg/L concentrations, respectively. Conclusion Present study expands the paradigm of surfactin-like biosurfactants produced by novel isolate Bacillus nealsonii S2MT for achieving efficient and environmentally acceptable soil remediation as compared to synthetic surfactants.

The continuous surge in global energy demand, fossil fuel depletion, and related climate change issues have oriented the worldwide researchers’ endeavors to the investigation and development of sustainable and co-effective technology to satisfy the global energy needs. Referring to the non-toxic properties of hydrogen, it is considered as a suitable renewable energy source that could replace fossil fuel-based energy. It is the cleanest energy carrier, combustible with high calorific value, high energy yield. Producing biohydrogen energy from renewable resources such as lignocellulosic agricultural residues could be a sustainable carbon-neutral most cost-effective approach. Dark fermentation has been widely applied as a promising eco-friendly technique to produce biohydrogen from agricultural residues. However, it has shown drawbacks owing to the recalcitrance of lignocellulose structure, and the accumulation of acid-rich intermediate by-products. Microbial electrolysis cells use bio-electrochemical reactions to upgrade H2 production in a dark fermentation reactor by promoting further decomposition of the generated volatile fatty acids. Therefore, integrating microbial electrohydrogenesis with dark fermentation can be a promising strategy to optimize the straw biomass conversion to biohydrogen. This review aims in delineating the structural composition and recalcitrance of the agricultural residues and their major effects on biohydrogen production. It summarizes all possible pre-treatment methods of the lignocellulosic agricultural residues; elucidates the stable operational conditions of microbial electrolysis cell and dark fermentation integrated system and discusses its performance for biohydrogen production. This study also reviewed the current technical challenges of this integrated system application and suggested sustainable solutions towards its industrial implementation.

The present study revealed biosurfactants production by a novel oil-degrading Pseudomonas sp. S2WE isolated from hydrocarbon enriched water sample, where the genus Pseudomonas (48.65%) was dominated amongst several other genera. Biosurfactants produced by this strain showed the great potential for surface tension reduction (SFT) and emulsification. The extracted crude biosurfactants were characterized using ultra-high-performance liquid chromatography-Mass Spectrometry (UHPLC-MS) and identified various mono-and di-rhamnolipids homologs from the mixture. Moreover, the lowest SFT 33.05 ± 0.1 mN/m and highest emulsification of 60.65 ± 0.64% were achieved from rhamnolipids produced from glycerol with urea. Compared to initial screening, almost (>87%) higher emulsification was observed. In addition, the biosurfactants were found highly stable at different environmental factors i.e. temperature (4 °C-121 °C), pH (3–10) and NaCl conc. (1–9%). The high stable rhamnolipids produced by new Pseudomonas sp. S2WE in this study could widely be used in enormous industrial as well as environmental applications.

Freshwater lake ecosystem is a reservior of valuable microbial diversity. It needs to be explored for addressing key environmental issues like petroleum-hydrocarbon contamination. In this work, the microbial communities (pre and post enriched with petroleum-hydrocarbons) from different layers of freshwater lake, i.e. surface water, sediments and deepwater, were explored through metagenomic and culture-dependent approaches. A total of 41 bacterial phyla were retrieved from pre-enriched samples, which were significantly reduced in enriched samples where Proteobacteria were dominant (87% to 100%) followed by Bacteroidetes (7.37%) and Verrucomicrobia (3.06%). The most dominant hydrocarbon-degrading genera were extensively verified as Pseudomonas (48.65%), Acinetobacter (45.38%), Stenotrophomonas (3.16%) and Brevundimonas (2.07%) in surface water (S1WCC); Acinetobacter (62.46%), Aeromonas (10.7%), Sphingobacterium (5.20%) and Pseudomonas (4.23%) in sediment (S2MCC); and Acinetobacter (46.57%), Pseudomonas (13.10%), Comamonas (12.93%), Flavobacterium (12.18%) and Enterobacter (9.62%) in deep water (S4WCC). Additionally, the maximum biodegradation of petroleum-hydrocarbons (i.e. used engine oil or UEO) was achieved by microbiome of S2MCC (67.60 ± 0.08%) followed by S4WCC (59.70 ± 0.12%), whereas only 36.80 ± 0.10% degradation was achieved by S1WCC microbiome. On the other hand, UEO degradation by cultivable biosurfactant-producing single cultures such as Pseudomonas sp. S2WE, Pseudomonas sp. S2WG, Pseudomonas sp. S2MS, Ochrobactrum sp. S1MM and Bacillus nealsonii S2MT showed 31.10 ± 0.08% to 40.50 ± 0.11% biodegradation. Comparatively, the biodegradation efficiency was found higher (i.e. 42.20 ± 0.12% to 56.10 ± 0.12%) in each consortia comprising of two, three, four, and five bacterial cultures. Conclusively, the isolated culturable biosurfactants-producing bacterial consortium of freshwater lake demonstrated >80% contribution in the total petroleum-hydrocarbons degradation by the natural microbiome of the ecosystem.

Cyanobacterial biomass is a promising natural resource for power generation, through the reactions bio-catalyzed by electrochemically active bacteria (EAB). However, the major limitation is the involvement of Microcystin-LR (MC-LR) in inhibiting EAB activation. In this work, toxic M. aeruginosa biomass was employed as analyte of a microbial fuel cell (MFC), and sodium acetate was applied as easy-to-biodegrade co-substrate to alleviate the MC-LR stress on EAB survival. The running stability was continuously enhanced with the increment of co-substrate concentration. The sufficient co-substrate supply (6.0 mM) eliminated the negative effects of MC-LR on the cyanobacteria biomass fed-MFC performance; it contributed 12.7% extension on the electric cyclic terms and caused the productions of the power density which was comparable and even 3.8% higher than its corresponding control (MFC treated with acetate alone). The co-substrate addition also increased coulombic efficiency by 60.1%, microcystin-LR removal efficiency increased by 64.7%, and diversified the microbial community with more species able to biodegrade the MC-LR, bio-transforming the metabolites and EAB. Microcystin-degrading bacteria, such as Sphingopyxis sp., Burkholderia-Paraburkholderia, and Bacillus sp., were remarkably increased, and EAB, including Shewanella sp., Desulfovibrio desulfuricans, Aeromonas hydrophila, were also much more enriched in co-substrate use protocol. Therefore, this study verified a co-substrate strategy for simultaneously eliminating MC-LR toxin and enhancing bioelectricity generation from cyanobacterial biomass via an MFC.