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We solve the problem of Apollonius by applying the Lorentz transformation within the framework of special relativity to go to a frame of simultaneity, where the solutions are the circumcenter. We transform back to get the solutions in a general frame. We show that the solutions in a general frame are the foci of the ellipse going through the centers of the given 3 circles. [ABSTRACT FROM AUTHOR]

The Rodl-Kaplice-Blanice fault system (RKB) of Variscan shear origin, repeatedly active since the Late Paleozoic to the Recent, is expressed by a number of lithological contacts, distinct geophysical gradients and many landforms. A general trend of the RKB as well as linear configuration of its internal architecture is fairly similar to those of topical near Rhine Graben and Alpine-Carpathian transition area as the two other consistent recently reactivated large-scale tectonic structures in the extended (thinned) crust of central Europe. In middle part of the RKB, the occurring linear topographic and geological features parallel to the main RKB sections point to the existence of a wide tectonic zone in the crust following the fault system. Our multidisciplinary study includes a summary of corresponding basic geological data, overview of seismic, regional geophysical and geomorphological conditions, primary model of recent kinematic activity in the RKB area derived from the space (Global Navigation Satellite System—GNSS) monitoring and terrestrial (repeated high precision levelling) geodetic data and comparison of these various information. The obtained knowledge indicates that the RKB is active up to ~ 1.0 mm horizontally and > 0.5 mm vertically. The fault system area in the Bohemian Massif can be subdivided into the three parts of diverse tectonic structure and block kinematics. Sinistral horizontal movements are highest near the southern surface sections (Rodl-Kaplice, Rudolfov and Drahotěšice faults), whereas noticeable vertical differentiation is going on mainly along the Blanice and Kouřim faults in the north where the RKB activity is gradually decreasing towards the extensive Elbe shear zone with transverse movements. The middle part of the RKB is dislocated by a large active transverse tectonic structure of the South Bohemian Basins (SBB) with variable horizontal velocity vectors of surface GNSS stations. Most of the weak regional earthquakes have been recorded west of the RKB. Besides faults of the SBB, these were mainly associated with the RKB-subparallel Lhenice fault. Based on the earthquake distribution and foci depths, the latter fault can have similar structural position as the RKB related to lower part of the Variscan level in the ~ 10–12 km depth. [ABSTRACT FROM AUTHOR]

SUMMARY: As in many other organisms, tRNA‐derived RNAs (tDRs) exist in plants and likely have multiple functions. We previously showed that tDRs are present in Arabidopsis under normal growth conditions, and that the ones originating from alanine tRNAs are the most abundant in leaves. We also showed that tDRs Ala of 20 nt produced from mature tRNAAla (AGC) can block in vitro protein translation. Here, we report that first, these tDRs Ala (AGC) can be found within peculiar foci in the cell that are neither P‐bodies nor stress granules and, second, that they assemble into intermolecular RNA G‐quadruplex (rG4) structures. Such tDR Ala rG4 structures can specifically interact with an Arabidopsis DEA(D/H) RNA helicase, the DExH1 protein, and unwind them. The rG4‐DExH1 protein interaction relies on a glycine‐arginine domain with RGG/RG/GR/GRR motifs present at the N‐terminal extremity of the protein. Mutations on the four guanine residues located at the 5′ extremity of the tDR Ala abolish its rG4 structure assembly, association with the DExH1 protein, and foci formation, but they do not prevent protein translation inhibition in vitro. Our data suggest that the sequestration of tDRs Ala into rG4 complexes might represent a way to modulate accessible and functional tDRs for translation inhibition within the plant cell via the activity of a specific RNA helicase, DExH1. Significance Statement: Small noncoding RNAs deriving from transfer RNAs are found in all living organisms, including plants but how they act within the plant cell remains largely unknown. We show here that some of them can be localized in specific foci and can adopt G‐quadruplex structures that can be unwound by a specific RNA helicase. [ABSTRACT FROM AUTHOR]

This paper offers a meta‐analysis scoping study of doctoral dissertations completed in the last 20 years in one doctoral programme of Educational Leadership (EL) at a higher education institution in Texas, aiming at identifying topical foci, epistemology, methodology and main themes. Therefore, we systematically collected, documented, securitised and analysed completed dissertations following the steps identified by PRISMA guidelines. The review aimed to answer the following four questions: (1) What are the general trends of doctoral dissertations in the studied programme? (2) What is the topical foci of these dissertations? (3) What are the prevailing epistemological constructs and genres informing this work? (4) What are the gaps in this research focus within the scope of educational leadership and policy? Based on our analysis, four main key themes were identified: educational leadership and policy, public education, equity and social justice, and race and ethnicity. Further implications, utility of research and future dissertations in educational leadership in this programme and other similar programmes are fully discussed. [ABSTRACT FROM AUTHOR]

Accurately characterizing DNA double-stranded breaks (DSBs) and understanding the DNA damage response (DDR) is crucial for assessing cellular genotoxicity, maintaining genomic integrity, and advancing gene editing technologies. Immunofluorescence-based techniques have proven to be invaluable for quantifying and visualizing DSB repair, providing valuable insights into cellular repair processes. However, the selection of appropriate markers for analysis can be challenging due to the intricate nature of DSB repair mechanisms, often leading to ambiguous interpretations. This comprehensively summarizes the significance of immunofluorescence-based techniques, with their capacity for spatiotemporal visualization, in elucidating complex DDR processes. By evaluating the strengths and limitations of different markers, we identify where they are most relevant chronologically from DSB detection to repair, better contextualizing what each assay represents at a molecular level. This is valuable for identifying biases associated with each assay and facilitates accurate data interpretation. This review aims to improve the precision of DSB quantification, deepen the understanding of DDR processes, assay biases, and pathway choices, and provide practical guidance on marker selection. Each assay offers a unique perspective of the underlying processes, underscoring the need to select markers that are best suited to specific research objectives. [ABSTRACT FROM AUTHOR]

• EEG source imaging (ESI) solutions obtained from a targeted density montage are similar to the ones calculated from a high-density montage. • This targeted density montage will alleviate HD-EEG's time and resource demand, making ESI more accessible to epilepsy centers. • Tangentially oriented foci show a statistically significant higher distance than radially oriented foci. High-density (HD) electroencephalography (EEG) is increasingly used in presurgical epilepsy evaluation, but it is demanding in time and resources. To overcome these issues, we compared EEG source imaging (ESI) solutions with a targeted density and HD-EEG montage. HD-EEGs from patients undergoing presurgical evaluation were analyzed. A low-density recording was created by selecting the 25 electrodes of a standard montage from the 83 electrodes of the HD-EEG and adding 8–11 electrodes around the electrode with the highest amplitude interictal epileptiform discharges. The ESI solution from this "targeted" montage was compared to that from the HD-EEG using the distance between peak vertices, sublobar concordance and a qualitative similarity measure. Fifty-eight foci of forty-three patients were included. The median distance between the peak vertices of the two montages was 13.2 mm, irrespective of focus' location. Tangential generators (n = 5/58) showed a higher distance than radial generators (p = 0.04). We found sublobar concordance in 54/58 of the foci (93%). Map similarity, assessed by an epileptologist, had a median score of 4/5. ESI solutions obtained from a targeted density montage show high concordance with those calculated from HD-EEG. Requiring significantly fewer electrodes, targeted density EEG allows obtaining similar ESI solutions as traditional HD-EEG montage. [ABSTRACT FROM AUTHOR]

Orthogonal families of bicircular quartics are naturally viewed as pairs of singular foliations of C ^ by vertical and horizontal trajectories of a non-vanishing quadratic differential. Yet the identification of these trajectories with real quartics in C P 2 is subtle. Here, we give an efficient, geometric argument in the course of updating the classical theory of confocal families in the modern language of quadratic differentials and the Edwards normal form for elliptic curves. In particular, we define a parameterized Edwards transformation, providing explicit birational equivalence between each curve in a confocal family and a fixed curve in normal form. [ABSTRACT FROM AUTHOR]

This paper expands on previous work on charter school typology and presents disparities in standardized test outcomes across models by using standardized Z-Scores weighted by NAEP performance. Analyses indicate that in ELA, Classical schools have the highest relative performance, followed by Montessori and Art schools. In math, Classical schools once again have the highest relative performance, followed by Montessori and STEM schools. For reasons discussed in the paper it is premature to posit causality, so the results should instead be viewed as descriptive. We suggest a more pluralistic testing framework may be appropriate when evaluating the performance of specialized schools. [ABSTRACT FROM AUTHOR]

Accurately characterizing DNA double-stranded breaks (DSBs) and understanding the DNA damage response (DDR) is crucial for assessing cellular genotoxicity, maintaining genomic integrity, and advancing gene editing technologies. Immunofluorescence-based techniques have proven to be invaluable for quantifying and visualizing DSB repair, providing valuable insights into cellular repair processes. However, the selection of appropriate markers for analysis can be challenging due to the intricate nature of DSB repair mechanisms, often leading to ambiguous interpretations. This comprehensively summarizes the significance of immunofluorescence-based techniques, with their capacity for spatiotemporal visualization, in elucidating complex DDR processes. By evaluating the strengths and limitations of different markers, we identify where they are most relevant chronologically from DSB detection to repair, better contextualizing what each assay represents at a molecular level. This is valuable for identifying biases associated with each assay and facilitates accurate data interpretation. This review aims to improve the precision of DSB quantification, deepen the understanding of DDR processes, assay biases, and pathway choices, and provide practical guidance on marker selection. Each assay offers a unique perspective of the underlying processes, underscoring the need to select markers that are best suited to specific research objectives.

Background: Leishmaniases are a group of vector-borne zoonotic diseases of public health relevance within the tropical and subtropical regions of the world. The state of Yucatan is a vulnerable and receptive area to localized cutaneous leishmaniasis (LCL) due to its proximity to the high-transmission endemic states of Campeche and Quintana Roo. Autochthonous cases of LCL caused by Leishmania (Leishmania) mexicana have been documented in the state, showing a geographical expansion of the disease. Materials and Methods: Using CO2-supplemented Centers for Disease Control and Prevention light traps and Shannon traps, we captured anthropophilic sandflies in the surroundings of a locality with recent records of autochthonous cases of LCL. Sandflies carrying Leishmania DNA were evidenced using PCR. Results: A total of 140 Phlebotominae (Diptera: Psychodidae) females of four species were captured: Lutzomyia (Tricholateralis) cruciata (Coquillett), Psathyromyia (Psathyromyia) shannoni (Dyar), Lutzomyia (Lutzomyia) longipalpis (Lutz and Neiva), and Dampfomyia (Coromyia) deleoni (Fairchild and Hertig). Molecular results showed that 6.1% (95% confidence interval [CI] = 2.3–12.9%) of Lu. cruciata and 43.8% (95% CI = 19.8–70.1%) of Pa. shannoni showed evidence of carrying L. (L.) mexicana DNA. Conclusion: We provide evidence of anthropophilic sandflies carrying L. mexicana DNA in a municipality with recorded autochthonous cases of LCL caused by this parasite species in the state of Yucatan, suggesting the emergence of new focus of LCL in Mexico. [ABSTRACT FROM AUTHOR]

Orthogonal families of bicircular quartics are naturally viewed as pairs of singular foliations of C^ by vertical and horizontal trajectories of a non-vanishing quadratic differential. Yet the identification of these trajectories with real quartics in CP2 is subtle. Here, we give an efficient, geometric argument in the course of updating the classical theory of confocal families in the modern language of quadratic differentials and the Edwards normal form for elliptic curves. In particular, we define a parameterized Edwards transformation, providing explicit birational equivalence between each curve in a confocal family and a fixed curve in normal form.

In this section of Resonance, we invite readers to pose questions likely to be raised in a classroom situation. We may suggest strategies for dealing with them, or invite responses, or both. "Classroom" is equally a forum for raising broader issues and sharing personal experiences and viewpoints on matters related to teaching and learning science. In this article, we will establish a simple proof for the Siebeck-Marden theorem through a lemma that directly calculates the foci of the Steiner circumellipse. [ABSTRACT FROM AUTHOR]

Aim. The assessment of the effectiveness, safety and tolerance of the drug Riamilovir for emergency drug prevention in the foci of a new coronavirus infection (COVID-19). Materials and methods. The trial included 113 persons aged 18 years and older who had level 1 contacts with patients with a new coronavirus infection (COVID-19), who had not previously been ill, with negative PCR results for COVID-19. Results. The high effectiveness, safety and good tolerance of the preventive use of the drug Riamilovir for the period of 20 days of taking a prophylactic dose of 1 capsule (250 mg) per day in the foci of COVID-19 has been established. Conclusion. The effectiveness and safety of the preventive use of the drug Riamilovir allow to recommend it for emergency drug prophylaxis in contact persons in the foci of a new coronavirus infection (COVID-19).

Prior studies evaluating features of foci associated with malignancy have not been conclusive. This study evaluates foci that were deemed suspicious and assesses multiple imaging and clinical findings with the goal of identifying criteria that can increase diagnostic confidence when evaluating foci on breast MRI. After Institutional Review Board approval, a retrospective chart review was performed to identify patients who underwent an image-guided biopsy of an enhancing focus. To be included in the study, a breast MRI performed between 2012 and 2019 must have been classified as suspicious for an enhancing focus or foci, and a biopsy using imaging guidance must have been subsequently performed. Patient and imaging characteristics as well as the corresponding biopsy results were recorded and statistically analyzed. There were 74 patients with 85 foci of enhancement who underwent biopsy within the study period. Thirteen of the 85 foci yielded malignant results for an overall positive predictive value of 15.3% (95% confidence interval: 7.7–22.9%). Additionally, twenty-six of the 85 cases (30.6%) yielded high risk lesions. There was a statistically significant negative correlation between screening breast MRIs and biopsies that yielded cancer or atypia (p = 0.04). There was also a significant association between foci and malignant results if the focus was in the same quadrant of a known malignancy (p = 0.001). Clinical information, such as the indication for a breast MRI or the location of a focus relative to a known cancer, can play an important role in evaluating foci of enhancement. Diagnostic confidence in identifying suspicious foci can be aided by incorporating clinical context with imaging findings deemed suspicious by prior research studies. • Enhancing foci on breast MRIs sometimes pose diagnostic dilemmas. • Foci on screening MRIs may be less suspicious than foci on diagnostic MRIs. • Foci in the same quadrant of a known malignancy may be more suspicious. [ABSTRACT FROM AUTHOR]

We seek to build a framework with which researchers can track the trends in charter school specialization and parents can better understand their options. This report examines the entire charter school population operating in the 2018–19 school year and calculates the rate of specialization among charter schools based on key terms found on their websites and marketing materials. For the purposes of this paper, we defined a "focus" as a deliberate form of specialization. We categorized charter schools' foci into three categories. Schools with a "curricular focus," utilize a specialized core curriculum beyond the standard history, science, ELA, and math. Schools with a "pedagogical focus," utilize a specialized method of teaching. Lastly, schools with a "student body focus," serve a nonstandard student body. Within these three categories of foci, we identified nine unique curricular foci, eleven pedagogical foci, and five student body foci. Because many schools have multiple foci across the three categories, we considered the complete combination of a school's specialized foci to be their "model." We found a total of 68 specialized models. In total, roughly two thirds of all charter schools use a specialized model of some sort. The top specialized models were single-focus models: "Personalized Learning" (389), "High Expectations" (370), "STEM" (368), "Classical" (257), and "Art" (223). We provide a comprehensive list of all school models, their definitions, and other relevant information in this report. [ABSTRACT FROM AUTHOR]

At present, the attention of many researchers is focused on the disseminated tuberculosis problem due to its relevance. In some regions of Russia, disseminated tuberculosis is fairly common form of pulmonary tuberculosis. Pulmonary tuberculosis combines processes of different origins, which result from the hematogenous, bronchogenic, and lymphogenous spread of Mycobacterium tuberculosis, which is the reason for a variety of clinical and radiographic manifestations. In particular, the atypical localization of foci in the lower and middle lung segments and the atypical distribution of pulmonary lobular foci frequently lead to diagnostic errors and present problems in their interpretation. This investigation is devoted to the different variants of lymphogenous dissemination of tuberculosis with an emphasis on modern radiodiagnosis.

Myotonic dystrophy type 1 (DM1) is a spliceopathy related to the mis-splicing of several genes caused by sequestration of nuclear transcriptional RNA-binding factors from non-coding CUG repeats of DMPK pre-mRNAs. Dysregulation of ryanodine receptor 1 (RYR1), sarcoplasmatic/endoplasmatic Ca2+-ATPase (SERCA) and α1S subunit of voltage-gated Ca2+ channels (Cav1.1) is related to Ca2+ homeostasis and excitation-contraction coupling impairment. Though no pharmacological treatment for DM1 exists, aberrant splicing correction represents one major therapeutic target for this disease. Resveratrol (RES, 3,5,4′-trihydroxy-trans-stilbene) is a promising pharmacological tools for DM1 treatment for its ability to directly bind the DNA and RNA influencing gene expression and alternative splicing. Herein, we analyzed the therapeutic effects of RES in DM1 myotubes in a pilot study including cultured myotubes from two DM1 patients and two healthy controls. Our results indicated that RES treatment corrected the aberrant splicing of RYR1, and this event appeared associated with restoring of depolarization-induced Ca2+ release from RYR1 dependent on the electro-mechanical coupling between RYR1 and Cav1.1. Interestingly, immunoblotting studies showed that RES treatment was associated with a reduction in the levels of CUGBP Elav-like family member 1, while RYR1, Cav1.1 and SERCA1 protein levels were unchanged. Finally, RES treatment did not induce any major changes either in the amount of ribonuclear foci or sequestration of muscleblind-like splicing regulator 1. Overall, the results of this pilot study would support RES as an attractive compound for future clinical trials in DM1. Ethical approval was obtained from the Ethical Committee of IRCCS Fondazione Policlinico Universitario A. Gemelli, Rome, Italy (rs9879/14) on May 20, 2014.

The results of the archaeological exploration of the Roman vicus of Falacrinae, placed in the Upper Sabina 78 miles north-east of Rome, represent excellent first-hand material for testing the concept of “rurification” of religion. The frequentation of the area goes back over time at least to the late Neolithic, but it is only in the Archaic period that a temple was built, soon converting itself into a sort of pole of attraction of the local community. After the Roman conquest (290 BCE), an entire village gradually arose around the monument. 129 sacrificial foci, dated between the late 3rd and the second half of the 1st cent. BCE (probably linked with the festivals of the Feriae Sementivae, Paganalia or Compitalia), and few burials (suggrundaria) belonging to perinatal foetuses of 30/40 weeks of gestation, dated during the 2nd and the first half of the 1st cent. BCE, are the most intriguing ritual practices that the excavations have been able to identify. The analysis of these practices encourages to conclude that the local rural communities: 1) adopted group-styles of religious grouping significantly different from those taking place in urban contexts; 2) could strongly modify hierarchies and rituals performed in the cities; 3) cannot necessarily be considered as “deviant” from the normative point of view; 4) could easily negotiate between local religious traditions and urban patterns.

Epithelial cells form epithelial tissue structures by joining together via intercellular adhesion structures composed of intercellular adhesion factors such as zona occludins-1 (ZO-1). Epithelial cells are polarized at the apical and basal regions, and are bordered by intercellular adhesion structures called tight junctions; the organelles within epithelial cells are distributed asymmetrically. Maintenance of this asymmetry in normal epithelial cells is essential for normal cytoskeletal remodeling, movement, and cell division. The key factor regulating cell polarity is called partitioning-defective protein 3 (Par3). Abnormalities in cell polarity and intercellular adhesion are common features of many cancer tissues. Mutation and loss of cell polarity regulators contributes to the immortalization of normal cells and to the malignant transformation of cancer cells. In this study, we investigated the relationship between the subcellular localization of Par3 and ZO-1 and clinicopathological features of lung squamous cell carcinoma (lung SqCC). Both molecules were localized to the cell membrane in normal lung tissue, but the levels were lower at this location in pulmonary tumor tissue compared with normal lung tissue. Both Par3 and ZO-1 accumulated in clusters on the cell membrane (hereinafter, "foci"). Tumor size, recurrence rate, and mortality rate were significantly higher in patients with Par3 foci compared to those without Par3 foci. Rates of lymph node metastasis, recurrence, and mortality were significantly higher in patients with ZO-1 foci than in those without ZO-1 foci. The expression of Par3 and ZO-1 mRNA was not s ignificantly different in s amples from p atients with foci versus those without. These results strongly suggest that the presence of Par3 and ZO-1 foci on the membrane may be a useful prognostic marker for lung SqCC. [ABSTRACT FROM AUTHOR]

Malignant transformation of cells is typically associated with increased proliferation, loss of contact inhibition, acquisition of anchorage-independent growth potential, and the ability to form tumors in experimental animals(1). In NIH 3T3 cells, the Ras signal transduction pathway is known to trigger many of these events, what is known as Ras transformation. The introduction of an overexpressed gene in NIH 3T3 cells may promote morphological transformation and loss of contact inhibition, which can help determine the oncogenic potential of that gene of interest. An assay that provides a straightforward method to assess one aspect of the transforming potential of an oncogene is the Focus Formation Assay (FFA)(2). When NIH 3T3 cells divide normally in culture, they do so until they reach a confluent monolayer. However, in the presence of an overexpressed oncogene, these cells can begin to grow in dense, multilayered foci(1) that can be visualized and quantified by crystal violet or Hema 3 staining. In this article we describe the FFA protocol with retroviral transduction of the gene of interest into NIH 3T3 cells, and how to quantify the number of foci through staining. Retroviral transduction offers a more efficient method of gene delivery than transfection, and the use of an ecotropic murine retrovirus provides a biosafety control when working with potential human oncogenes.

The transition from the control phase to elimination of malaria in China through the national malaria elimination programme has focussed attention on the need for improvement of the surveillance- response systems. It is now understood that routine passive surveillance is inadequate in the parasite elimination phase that requires supplementation by active surveillance in foci where cluster cases have occurred. This study aims to explore the spatial clusters and temporal trends of malaria cases by the multivariate auto-regressive state-space model (MARSS) along the border to Myanmar in southern China. Data for indigenous cases spanning the period from 2007 to 2010 were extracted from the China’s Infectious Diseases Information Reporting Management System (IDIRMS). The best MARSS model indicated that malaria transmission in the study area during 36 months could be grouped into three clusters. The estimation of malaria transmission patterns showed a downward trend across all clusters. The proposed methodology used in this study offers a simple and rapid, yet effective way to categorize patterns of foci which provide assistance for active monitoring of malaria in the elimination phase.

BackgroundDuctal carcinoma in situ with microinvasion (DCISM) was defined as one or more foci of invasion beyond the basement membrane within 1 mm. The size of primary lesion is associated with axillary status and prognosis in patients with invasive breast cancer; thus, it is of interest to determine whether multiple foci of microinvasion are associated with a higher risk of positive axillary status or worse long-term outcomes in patients with DCISM.MethodsThis study identified 359 patients with DCISM who had undergone axillary evaluation at our institute from January 2006 to December 2015. Patients were categorized as one focus or multiple foci (≥2 foci) according to the pathological results. Clinicopathological features, axillary status, and disease-free survival rate were obtained and analyzed.ResultsOf 359 patients, 233 (64.90%) had one focus of microinvasion and 126 (35.10%) had multiple foci. Overall, 242 (67.41%) and 117 (32.59%) patients underwent sentinel lymph nodes biopsy (SLNB) and axillary lymph nodes dissection (ALND), respectively. Isolated tumor cells were found in four (1.11%) patients and axillary metastasis rate was 2.51%. Neither axillary evaluation methods (P = 0.244) nor axillary metastasis rate (P = 0.559) was significantly different between patients with one focus and multiple foci. In univariate analysis, patients with multiple foci tended to have larger tumor size (P < 0.001), higher nuclear grade (P = 0.001), and higher rate of lymphatic vascular invasion (P = 0.034). Also, the proportion of positive HER2 (P = 0.027) and Ki67 level (P = 0.004) increased in patients with multiple foci, while in multivariate analysis, only tumor size showed significant difference (P = 0.009). Patients with multiple foci were more likely to receive chemotherapy (56.35 vs 40.77%; P = 0.028). At median 5.11 years follow-up, overall survival rate was 99.36%. Patients with multiple microinvasive foci had worse disease-free survival rate compared with one-focus patients (98.29 vs 93.01%, P = 0.032).ConclusionEven though the numbers of microinvasion were different and patients with multiple foci of microinvasion tended to have larger tumor size, there was no higher risk of axillary involvement compared with patients with one focus of microinvasion, while patients with multiple microinvasive foci had worse DFS rate. Thus, DCISM patients with multiple foci of microinvasion may be the criterion for more aggressive local–regional treatment. Optimization of adjuvant therapy in DCISM patients is required.

This study investigates language attitudes expressed by public university students in India at various layers of agentive positionality as an integral part of their dynamic language ecologies. This is achieved through the qualitative analysis of three focus group interviews conducted at three public universities in India with the object of eliciting the students' language attitudes towards English and its use in India. This research perceives students as agents and explores their attitudes in the context of a language ecology Such an enquiry into language attitudes forms a part of a broader exploration into the nature of agency, agentive responses and their situatedness in an LPP context. It adds to existing scholarship on LPP by reinterpreting the meaning(s) of agency with a critical focus on 'ground-up' descriptions of language experiences. In this study, the researchers argue that invisible language planners' as Pakir describes,and interested stakeholders such as university students, provide a critical basis for the study of LPP in context. The authors argue that agency is multi-layered, and that agentive positionality is relative to agentive foci. Such reconfigurations of agency mark a shift from the view that language policies are the starting point for understanding an LPP ecology. [ABSTRACT FROM AUTHOR]

Background: Ductal carcinoma in situ with microinvasion (DCISM) was defined as one or more foci of invasion beyond the basement membrane within 1 mm. The size of primary lesion is associated with axillary status and prognosis in patients with invasive breast cancer; thus, it is of interest to determine whether multiple foci of microinvasion are associated with a higher risk of positive axillary status or worse long-term outcomes in patients with DCISM. Methods: This study identified 359 patients with DCISM who had undergone axillary evaluation at our institute from January 2006 to December 2015. Patients were categorized as one focus or multiple foci (≥2 foci) according to the pathological results. Clinicopathological features, axillary status, and disease-free survival rate were obtained and analyzed. Results: Of 359 patients, 233 (64.90%) had one focus of microinvasion and 126 (35.10%) had multiple foci. Overall, 242 (67.41%) and 117 (32.59%) patients underwent sentinel lymph nodes biopsy (SLNB) and axillary lymph nodes dissection (ALND), respectively. Isolated tumor cells were found in four (1.11%) patients and axillary metastasis rate was 2.51%. Neither axillary evaluation methods (P = 0.244) nor axillary metastasis rate (P = 0.559) was significantly different between patients with one focus and multiple foci. In univariate analysis, patients with multiple foci tended to have larger tumor size (P < 0.001), higher nuclear grade (P = 0.001), and higher rate of lymphatic vascular invasion (P = 0.034). Also, the proportion of positive HER2 (P = 0.027) and Ki67 level (P = 0.004) increased in patients with multiple foci, while in multivariate analysis, only tumor size showed significant difference (P = 0.009). Patients with multiple foci were more likely to receive chemotherapy (56.35 vs 40.77%; P = 0.028). At median 5.11 years follow-up, overall survival rate was 99.36%. Patients with multiple microinvasive foci had worse disease-free survival rate compared with one-focus patients (98.29 vs 93.01%, P = 0.032). Conclusion: Even though the numbers of microinvasion were different and patients with multiple foci of microinvasion tended to have larger tumor size, there was no higher risk of axillary involvement compared with patients with one focus of microinvasion, while patients with multiple microinvasive foci had worse DFS rate. Thus, DCISM patients with multiple foci of microinvasion may be the criterion for more aggressive local–regional treatment. Optimization of adjuvant therapy in DCISM patients is required. [ABSTRACT FROM AUTHOR]

Abstract Background Despite the biological plausibility of hotspots fueling malaria transmission, the evidence to support this concept has been mixed. If transmission spreads from high burden to low burden households in a consistent manner, then this could have important implications for control and elimination program development. Methods Data from a longitudinal cohort in The Gambia was analyzed. All consenting individuals residing in 12 villages across the country were sampled monthly from June (dry season) to December 2013 (wet season), in April 2014 (mid dry season), and monthly from June to December 2014. A study nurse stationed within each village recorded passively detected malaria episodes between visits. Plasmodium falciparum infections were determined by polymerase chain reaction and analyzed using a geostatistical model. Results Household-level observed monthly incidence ranged from 0 to 0.50 infection per person (interquartile range = 0.02–0.10) across the sampling months, and high burden households exist across all study villages. There was limited evidence of a spatio-temporal pattern at the monthly timescale irrespective of transmission intensity. Within-household transmission was the most plausible hypothesis examined to explain the observed heterogeneity in infections. Conclusions Within-village malaria transmission patterns are concentrated in a small proportion of high burden households, but patterns are stochastic regardless of endemicity. Our findings support the notion of transmission occurring at the household and village scales but not the use of a targeted approach to interrupt spreading of infections from high to low burden areas within villages in this setting.

Abstract Background China has initiated the National Malaria Elimination Action Plan, which aims to eliminate malaria by 2020. However, the transmission of malaria occurs sporadically or in distinct foci, which greatly hampers progress toward elimination in China and other countries. The object of this study was to foci categorization and evaluates whether the response met the requirements issued by the nation or WHO. Methods Residual transmissions were investigated and located with fine spatial resolution mapping from parasitological confirmed malaria cases by use of routine national surveillance data. The “1–3-7” timeframes were monitored for each focus between 2012 and 2015. Each focus was identified, and the application of appropriate measures was evaluated. Results A total of 5996 indigenous cases were recorded between 2010 and 2015; during this period, the number of cases declined by 99.1% (2010, n = 4262; 2015, n = 39). Most indigenous cases (92.5%) were reported in Anhui (n = 2326), Yunnan (n = 1373), Henan (n = 930), Hubei (n = 459), and Guizhou (n = 458). The temporal distribution showed that the indigenous malaria cases were clustered during the period of May to August. A total of 320 foci were carefully investigated and analyzed: 24 were active foci; 72, residual non-active foci; and 224 cleared-up foci. For the foci response evaluation, all the active foci were investigated within 7 days, while 80.2% of the residual non-active foci were responded within 7 days. In addition, reactive case detection (RACD) was carried out with 92.9% of the active foci and vector investigation carried out with 75%. For residual non-active foci, RACD was carried out with 83.2% and vector investigation with 78.2% of the foci. Conclusions This study used nationwide data to categorize foci in China and evaluate the response of these areas during the control and elimination phases. Our approach stratifies future control responses by identifying those locations where the elimination of endemic transmission is needed, such as in the counties at the China–Myanmar border and in Tibet. In addition, this study will help local CDC staff to reassess their needs and responses against different types of foci during the elimination and post-elimination phases.

The present study aimed to investigate the genotoxicity in erythrocytes induced after exposure of medaka (Oryzias latipes) to 4-nonylphenol (4-NP). Adult female medaka fish were exposed to 4-NP at three sublethal concentrations for 15 days to compare their sensitivity with that of catfish as an aquatic model. Comet assay and γ-H2AX were used as biomarkers to detect DNA damage in erythrocytes. Exposure to 4-NP resulted in an increase in the tail moment in a dose-dependent manner. The highest level of DNA damage was recorded after exposure to 100 μg/l 4-NP. The number of foci was increased after exposure to 4-NP, indicating damage to DNA. The present results confirmed the high level of morphological alterations and apoptosis of erythrocytes detected in the first part of this study. 4-NP induced genotoxic effects in medaka, which were found to be more sensitive than catfish after exposure to 4-nonylphenol. [ABSTRACT FROM AUTHOR]

Analysis of the dynamics of modern desertification of the Northwestern Caspian with the use of geoinformation technologies and aerospace data showed that the area of degraded and desertified lands in the region increases due to an increase in the anthropogenic factor (pasture load). With a relatively stable number of animals, the role of climatic factors increases, which leads to an increase in the speed and amplitude of changes in the area of desertification foci, both in the direction of a decrease with sufficient moisture and an increase in dry years. A significant influence is exerted by fires, which lead not only to a decrease in projective cover but also to a depletion of the species composition of vegetation. The study and mapping of the relief made it possible to establish spatially defined geomorphological features of the territory as a factor affecting local moisture and, accordingly, the stability of ecosystems and the possibility of phytomelioration. [ABSTRACT FROM AUTHOR]

Background and Aim: Massive hepatic necrosis is a rare but often fatal complication of various liver injuries. Nevertheless, some patients can survive by spontaneous hepatic regeneration. It is known that surviving hepatocytes and/or progenitor cells can participate in this process but the mechanism of hepatic recovery is vague. Methods: We examined 13 explanted human livers removed for acute liver failure. Combined immunohistochemistry, digital image analysis, and three‐dimensional reconstruction of serial sections were applied. Results: Two patterns of regeneration could be distinguished. In livers with centrilobular necrosis, the surviving injured periportal hepatocytes started to proliferate and arrange into acinar structures and expressed α‐fetoprotein. If the injury wiped out almost all hepatocytes, large areas of parenchymal loss were invaded by an intense ductular reaction. The cells at the distal pole of the ductules differentiated into hepatocytes and formed foci organized by the branches of the portal vein. The expanding foci often containing complete portal triads were arranged around surviving central veins. Their fusion eventually could be an attempt to re‐establish the hepatic lobules. Conclusions: Regeneration of human livers following massive hepatic necrosis can occur in two ways—either through proliferation of α‐fetoprotein‐positive acinary‐arranged hepatocytes or through ductular progenitor cells, with the latter being less efficient. Further investigation of these regenerative pathways may help identify biomarkers for likelihood of complete regeneration and hence have therapeutic implications. [ABSTRACT FROM AUTHOR]

Introduction. Measles still poses a threat to the inhabitants of the planet. In different regions of the world and the Russian Federation are recorded instances of contamination and subsequent infection with formation of foci with a significant number of victims. Measles has a high reproduction rate of the infection. The purpose of this study was to determine the main characteristics of the epidemic process of measles and its determinants in realtime for direct and indirect control of epidemic process. Materials and methods. The authors analyzed the data of official registration of the incidence of measles the population of Ekaterinburg from 1988 to 2016, and medical records of cases of measles in 2016. We used epidemiological, clinical and statistical research methods. Results. The authors found that the epidemic process of measles has undergone significant changes. Measles was not registered in the city from 2001 to 2015, the unit drifts measles-endemic territories didn't leak. However, the situation changed at the end of 2016. It was 72 clinically and laboratory confirmed case of measles in a period of 11 weeks. The largest proportion of cases were in children who are not vaccinated against measles. The average age of infection in children was 3.8 years. 59 measles epidemic foci were formed during the flash. Foci were registered in families, educational institutions and health care organizations. The most active spread of the infection was in medical organizations. The spread was associated with the presence of susceptible children and adults, serious shortcomings in terms of timely diagnostics of measles in the first and subsequent cases, violations in the organization of antiepidemic measures. Conclusion. 1. Despite positive shifts in the direction of measles elimination, it remains an actual infection, reserving opportunities for distribution among unvaccinated adults and children. 2. A special feature of the outbreak of measles in the territory of Yekaterinburg was the spread in medical organizations with the formation of secondary foci along the routes of following sick children and adults. The share of foci in medical organizations was 15.2% of the number of all foci, and the number of cases in any - 70.8% of registered cases.3. The significance of previously vaccinated cases as potential sources of infection in the foci is not high, the incidence rate when in contact with them is 0.53 ± 0.15, whereas in contact with previously not vaccinated - 7.94 ± 0.56.

A massive particle under the influence of a constant gravitational force that is bouncing inside an ideal reflecting mirror described by some function f (x) is considered. For the associated flight trajectories we derive the parametric curves, named foci curves. All foci points of the parabolas for a given initial position and energy lie on these curves. From these foci curves the associated flight parabola envelopes are derived resulting, together with the mirror surface, in a confined domain for all possible particle trajectories in the non-periodic orbit case. The general results are briefly discussed and visualized for three concrete mirror surfaces. • Foci curves of flight parabolas. • Confined domains. • Application to various examples. [ABSTRACT FROM AUTHOR]

Proteins can aggregate in response to stresses, including hyperosmotic shock. Formation and disassembly of aggregates is a relatively slow process. We describe a novel instant response of the cell to hyperosmosis, during which chaperones and other proteins form numerous foci with properties uncharacteristic of classical aggregates. These foci appeared/disappeared seconds after shock onset/removal, in close correlation with cell volume changes. Genome-wide and targeted testing revealed chaperones, metabolic enzymes, P-body components and amyloidogenic proteins in the foci. Most of these proteins can form large assemblies and for some, the assembled state was pre-requisite for participation in foci. A genome-wide screen failed to identify genes whose absence prevented foci participation by Hsp70. Shapes of and interconnections between foci, revealed by super-resolution microscopy, indicated that the foci were compressed between other entities. Based on our findings, we suggest a new model of cytosol architecture as a collection of numerous gel-like regions suspended in a liquid network. This network is reduced in volume in response to hyperosmosis and forms small pockets between the gel-like regions.

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In Brazil, glanders remains a serious problem, with the obligatory sacrifice of disease-positive animals without compensation. Each year, glanders cases are reported in several regions of the country, causing severe economic losses and trade restrictions. The present study describes and discusses the occurrence of glanders foci in Brazil during a 12-year period from 2005 to 2016. The highest frequency of reported affected holdings during the study period was in the northeast region. Moreover, during this period, the disease incidence in Brazil showed an overall increasing tendency. The number of affected holdings significantly increased during the last four years of the period, and more cases were noted during the months of May and June. Spatiotemporally, there are four high-risk glanders clusters: (1) cluster A (relative risk [RR = 6.51, P <.0001) involved the northeast region from March 2008 to February 2014; (2) cluster B (RR = 17.37, P <.0001) involved a southeast region state from March 2013 to June 2015; (3) cluster C (RR = 6.92, P <.0001) involved the states in the midwest, southeast, and south regions of Brazil from March 2015 to May 2016; and (4) cluster D (RR = 19.07, P <.0001) involved a north region state from October 2015 to April 2016. Only two states of the north region (Acre and Amapá) did not experience glanders during the study period. • Glanders remains a serious problem, with the obligatory sacrifice of disease-positive animals without compensation. • The present study describes and discusses the occurrence of glanders foci in Brazil in a 12-year period, 2005–2016. • The frequency of reported foci during the study period was highest in states of the Nordeste region. • There have been four spatiotemporal high-risk clusters. • These results show the worsening of the epidemiological situation for glanders in Brazil. [ABSTRACT FROM AUTHOR]

Myotonic dystrophy type 1 (DM1) is caused by CTG nucleotide repeat expansions in the 3'-untranslated region (3'-UTR) of the dystrophia myotonica protein kinase (DMPK) gene. The expanded CTG repeats encode toxic CUG RNAs that cause disease, largely through RNA gain-of-function. DM1 is a fatal disease characterized by progressive muscle wasting, which has no cure. Regenerative medicine has emerged as a promising therapeutic modality for DM1, especially with the advancement of induced pluripotent stem (iPS) cell technology and therapeutic genome editing. However, there is an unmet need to identify in vitro outcome measures to demonstrate the therapeutic effects prior to in vivo clinical trials. In this study, we examined the muscle regeneration (myotube formation) in normal and DM1 myoblasts in vitro to establish outcome measures for therapeutic monitoring. We found normal proliferation of DM1 myoblasts, but abnormal nuclear aggregation during the early stage myotube formation, as well as myotube degeneration during the late stage of myotube formation. We concluded that early abnormal nuclear aggregation and late myotube degeneration offer easy and sensitive outcome measures to monitor therapeutic effects in vitro. [ABSTRACT FROM AUTHOR]

The aim of this study was to identify and map the weed population in a no-tillage area. Geostatistical techniques were used in the mapping in order to assess this information as a tool for the localized application of herbicides. The area of study is 58.08 hectares wide and was sampled in a fixed square grid (which point spaced 50 m, 232 points) using a GPS receiver. In each point the weeds species and population were analyzed in a square with a 0.25 m2 fixed area. The species Ipomoea grandifolia, Gnaphalium spicatum, Richardia spp. and Emilia sonchifolia have presented no spatial dependence. However, the species Conyza spp., C. echinatus and E. indica have shown a spatial correlation. Among the models tested, the spherical model has shown had a better fit for Conyza spp. and Eleusine indica and the Gaussian model for Cenchrus echinatus. The three species have a clumped spatial distribution. The mapping of weeds can be a tool for localized control, making herbicide use more rational, effective and economical.

Background: The 3D short tau inversion recovery (STIR) sequence is routinely used in clinical MRI to achieve robust fat suppression. However, the performance of the commonly used adiabatic inversion pulse, hyperbolic secant (HS), is compromised in challenging areas with increased B0 and B1 inhomogeneities, such as brachial plexus at 3T.Purpose: To demonstrate the frequency offset corrected inversion (FOCI) pulse as an efficient fat suppression STIR pulse with increased robustness to B0 and B1 inhomogeneities at 3T, compared to the HS pulse.Study Type: Prospective.Subjects/phantom: Initial evaluation was performed in phantoms and one healthy volunteer by varying the B1 field, while subsequent comparison was performed in three healthy volunteers and five patients without varying the B1 .Field Strength/sequence: 3T; 3D TSE-STIR with HS and FOCI pulses.Assessment: Brachial plexus images were qualitatively evaluated by two musculoskeletal radiologists independently using a four-point grading scale for fat suppression, shading artifacts, and nerve visualization.Statistical Test: The Wilcoxon signed-rank test with P < 0.05 was considered statistically significant.Results: Simulations and phantom experiments demonstrated broader bandwidth (2.5 kHz vs. 0.83 kHz, increased B0 robustness) at the same adiabatic threshold and lower adiabatic threshold (5 μT vs. 7 μT at 3.5 ppm, increased B1 robustness) at the same bandwidth with the FOCI pulse compared to the HS pulse With increased bandwidth, the FOCI pulse achieved robust fat suppression even at 50% of maximum B1 strength, while the HS pulse required >75% of maximum B1 strength. Compared to the standard 3D TSE-STIR with HS pulse, the FOCI pulse achieved uniform fat suppression (P < 0.05), better nerve visualization (P < 0.05), and minimal shading artifacts (P < 0.01) in brachial plexus at 3T.Data Conclusion: The FOCI pulse has increased robustness to B0 and B1 inhomogeneities, compared to the HS pulse, and enables uniform fat suppression in brachial plexus at 3T.Level Of Evidence: 1 Techinical Efficacy: Stage 1 J. Magn. Reson. Imaging 2018;48:1104-1111. [ABSTRACT FROM AUTHOR]

Optineurin (OPTN) plays an important role in membrane trafficking processes such as exocytosis and autophagy. The sizes and rate of formation of accumulated structures comprising OPTN, such as foci or inclusion bodies (IBs), are often disrupted by amyotrophic lateral sclerosis (ALS) and glaucoma-associated mutants of OPTN. Therefore, methods for the quantitative measurement of the size of the accumulated structure are necessary. Here, we show that, using spatial image correlation spectroscopy (ICS), the average diameter of accumulated structures of the wild-type and disease-associated mutants in living cells may be easily determined. Although OPTN was found to frequently form foci in the cytoplasm, regardless of ALS- and glaucoma-associated mutation, the diameter of OPTN foci decreased in an ALS-associated mutant and increased in a glaucoma-associated mutant. However, a portion of cells carried IBs of the ALS-associated mutant that were larger than micrometre and ellipse-like shape, suggesting that this mutant accumulates non-uniformly in the IBs. The findings suggest that changes in their accumulation, determined via quantitative comparison of the OPTN foci and IBs in the cells, are involved in pathological features of ALS. In addition, this method enables rapid comparison of the average sizes of various other intracellular structures such as granules. [ABSTRACT FROM AUTHOR]

Yeast Cdc13 protein (related to human CTC1) maintains telomere stability by preventing 5′-3′ end resection. While Cdc13 and Yku70/Yku80 proteins appear to prevent excessive resection, their combined contribution to maintenance of telomere ends across the genome and their relative roles at specific ends of different chromosomes have not been addressable because Cdc13 and Yku70/Yku80 double mutants are sickly. Using our PFGE-shift approach where large resected molecules have slower p ulse f ield g el e lectrophoresis mobilities, along with methods for maintaining viable double mutants, we address end-resection on most chromosomes as well as telomere end differences. In this global approach to looking at ends of most chromosomes, we identify chromosomes with 1-end resections and end-preferences. We also identify chromosomes with resection at both ends, previously not possible. 10–20% of chromosomes exhibit PFGE-shift when cdc13-1 cells are switched to restrictive temperature (37 °C). In yku70Δ cdc13-1 mutants, there is a telomere resection “storm” with approximately half the chromosomes experiencing at least 1-end resection, ∼10 kb/telomere, due to exonuclease1 and many exhibiting 2-end resection. Unlike for random internal chromosome breaks, resection of telomere ends is not coordinated. Telomere restitution at permissive temperature is rapid (<1 h) in yku70Δ cdc13-1 cells. Surprisingly, survival can be high although strain background dependent. Given large amount of resected telomeres, we examined associated proteins. Up to 90% of cells have ≥1 Rfa1 (RPA) focus and 60% have multiple foci when ∼30-40 telomeres/cell are resected. The ends are dispersed in the nucleus suggesting wide distribution of resected telomeres across nuclear space. The previously reported Rad52 nuclear centers of repair for random DSBs also appear in cells with many resected telomere ends, suggesting a Rad52 commonality to the organization of single strand ends and/or limitation on interactions of single-strand ends with Rad52. [ABSTRACT FROM AUTHOR]

The host-specific and typical species, the intermediate hosts available in a certain territory, the anthropogenic factors affecting the formation of the helminth fauna, and the period of stability of their newly formed mixed foci are reported for the first time as composite component factors which can influence the formation of the helminth fauna in domestic carnivores. The performed bioecological surveys in the areas with the different environmenal characteristics in Azerbaidjan have ascertained that the helminth fauna in the domestic carnivores can be formed of 14 helminth species specific to certain analyzed animals, 24 helminth species typical of a certain area, 41 species existing in the determined intermediate hosts, and 13 helminth species occurring in the newly formed mixed infection foci. Fourteen helminth species have a potential to cause the epidemics that can affect humans. [ABSTRACT FROM AUTHOR]

Growing body of evidence shows that extra adiposity influences on the progression of multiple cancers, including breast cancer. The aim of this study is to investigate whether obesity correlates with mammary tumor development in vitro and in vivo . We found that obesity-related mediators, 3T3-L1 adipocyte conditioned medium, enhanced formation of cancerous foci induced by the carcinogen 7,12-Dimethylbenz[ a ]anthracene (DMBA) in NIH/3T3 fibroblasts, in vitro . Additionally, we tested the effect of obesity in mouse model of DMBA-induced breast cancer. C57BL/6J female mice were fed a low fat (LF), or high fat (HF) diet, and DMBA was administered by oral gavage (LF plus DMBA [LFD] and HF plus DMBA [HFD]). Our results indicated that HFD mouse developed a tumor which weight was 169mg, whereas the LFD mouse developed a tumor weight of 77mg. Histological analysis of the mammary tumor from HFD group showed morphological aggressiveness and multiple cell type infiltration compared to LFD group. The epididymal adipose tissue from the DMBA groups showed more macrophage infiltration, polarized towards an M1 phenotype compared to the non-DMBA mice. HF mice showed less accumulation of M2 macrophages in the adipose tissue. In summary, obese mediators enhanced DMBA induced tumorigenesis in vitro and in vivo . [ABSTRACT FROM AUTHOR]

Background We previously demonstrated that persistent and long‐standing persistent atrial fibrillation is maintained by activation emanating from foci and breakthrough sites of different cycle lengths (CLs). The purpose of this study was to characterize the behavior of focal and nonrandom breakthrough activation identified during high‐density mapping of atrial fibrillation in these patients. Methods and Results During open heart surgery, we recorded activation from both atria simultaneously using 510 to 512 epicardial electrodes along with ECG lead II in 12 patients with persistent and long‐standing persistent atrial fibrillation. For each patient, analysis of 32 consecutive seconds of activation from identified focal (sustained and/or intermittent) and nonrandom breakthrough sites was performed. Multiple foci (sustained and/or intermittent) of different CLs were present in both atria in 11 of 12 patients; 8 foci were sustained, and 22 were intermittent. Temporal CL behavior of sustained foci varied over time (≤20 ms of the mean CL). For intermittent foci, no activation periods were due to a spontaneous pause (18 of 22) or activation of the focus by another wave front (11 of 22). All patients had breakthrough activation. Seven patients had 12 nonrandom breakthrough sites. Periods of no breakthrough activation were caused by a spontaneous pause (6 of 12 patients) or activation from another wave front (4 of 12) or were uncertain (5 of 12). Focal and nonrandom breakthrough activation sometimes produced repetitive “wannabe” (incomplete) reentry in 6 of 12 patients. Conclusions During persistent and long‐standing persistent atrial fibrillation, sustained foci manifested variable CLs. Spontaneous pauses or activation from other wave fronts explained the intermittency of foci and nonrandom breakthrough. Focal and nonrandom breakthrough activation occasionally produced wannabe reentry.