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Waste tire is a residue that accumulates over time due to the lack of processes for its recycling or transformation; one of the most recurrent anthropogenic activities, whether accidental or provoked, is open burning, whose contamination by ashes affects the environment and its ecosystems. The discharges from these fires could cause an imbalance in freshwater ecosystems, thus deteriorating the conditions necessary for the survival of aquatic species. This work analyzes waste tire ash's acute and chronic toxicity on the organism Daphnia magna. For the acute test, bioassays were carried out with three replicates for each of the eight elutriates, and for the chronic test, four groups of studies were formed with three replicates each. The acute test results showed an EC50 calculated for a 48-hour exposure of 5.83 g/L, and chronic test results revealed that the total reproduction of the population decreased over the 19 days in all treatments. [ABSTRACT FROM AUTHOR]